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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 203 |
| Acc36I | ACCTGC | 1 cut(s) | 1005 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 785 |
| AccI | GTMKAC | 1 cut(s) | 149 |
| AccII | CGCG | 1 cut(s) | 1010 |
| AciI | CCGC | 4 cut(s) | 74, 274, 848, 908 |
| AclWI | GGATC | 2 cut(s) | 1046, 1050 |
| AcoI | YGGCCR | 1 cut(s) | 275 |
| AcsI | RAATTY | 1 cut(s) | 770 |
| AcuI | CTGAAG | 2 cut(s) | 837, 1109 |
| AfaI | GTAC | 2 cut(s) | 838, 995 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 80, 649, 785, 1261 |
| AflIII | ACRYGT | 1 cut(s) | 1008 |
| AgsI | TTSAA | 4 cut(s) | 343, 539, 776, 941 |
| AluBI | AGCT | 3 cut(s) | 40, 321, 606 |
| AluI | AGCT | 3 cut(s) | 40, 321, 606 |
| Alw26I | GTCTC | 2 cut(s) | 120, 1138 |
| AlwI | GGATC | 2 cut(s) | 1046, 1050 |
| AlwNI | CAGNNNCTG | 4 cut(s) | 17, 643, 872, 997 |
| AoxI | GGCC | 4 cut(s) | 275, 397, 563, 1218 |
| ApeKI | GCWGC | 3 cut(s) | 20, 23, 26 |
| ApoI | RAATTY | 1 cut(s) | 770 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 771 |
| AspS9I | GGNCC | 3 cut(s) | 398, 563, 610 |
| AsuHPI | GGTGA | 1 cut(s) | 1048 |
| AvaII | GGWCC | 1 cut(s) | 610 |
| BaeGI | GKGCMC | 1 cut(s) | 656 |
| BbsI | GAAGAC | 1 cut(s) | 1240 |
| BbvCI | CCTCAGC | 1 cut(s) | 567 |
| BbvI | GCAGC | 3 cut(s) | 7, 10, 13 |
| BccI | CCATC | 2 cut(s) | 419, 1033 |
| BciVI | GTATCC | 1 cut(s) | 1258 |
| BclI | TGATCA | 2 cut(s) | 507, 634 |
| BcoDI | GTCTC | 2 cut(s) | 120, 1138 |
| BfaI | CTAG | 6 cut(s) | 513, 657, 684, 804, 857, 977 |
| BfmI | CTRYAG | 2 cut(s) | 150, 406 |
| BfuAI | ACCTGC | 1 cut(s) | 1005 |
| BfuI | GTATCC | 1 cut(s) | 1258 |
| BglII | AGATCT | 1 cut(s) | 967 |
| BisI | GCNGC | 5 cut(s) | 21, 24, 27, 74, 275 |
| BlsI | GCNGC | 5 cut(s) | 22, 25, 28, 75, 276 |
| Bme18I | GGWCC | 1 cut(s) | 610 |
| BmgT120I | GGNCC | 3 cut(s) | 398, 563, 610 |
| BmiI | GGNNCC | 1 cut(s) | 662 |
| BmrI | ACTGGG | 1 cut(s) | 658 |
| BmsI | GCATC | 2 cut(s) | 290, 989 |
| BmuI | ACTGGG | 1 cut(s) | 658 |
| BpiI | GAAGAC | 1 cut(s) | 1240 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 439, 471, 1291, 1323 |
| BpmI | CTGGAG | 1 cut(s) | 102 |
| Bpu10I | CCTNAGC | 1 cut(s) | 567 |
| BsaI | GGTCTC | 1 cut(s) | 1138 |
| BsaJI | CCNNGG | 1 cut(s) | 779 |
| BsaWI | WCCGGW | 1 cut(s) | 1196 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 165, 195, 918, 948 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 80, 649, 785, 1261 |
| Bse118I | RCCGGY | 1 cut(s) | 1216 |
| Bse1I | ACTGG | 2 cut(s) | 85, 653 |
| Bse3DI | GCAATG | 1 cut(s) | 1176 |
| BseDI | CCNNGG | 1 cut(s) | 779 |
| BseGI | GGATG | 2 cut(s) | 554, 1093 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 80, 649, 785, 1261 |
| BseMI | GCAATG | 1 cut(s) | 1176 |
| BseMII | CTCAG | 3 cut(s) | 581, 847, 1133 |
| BseNI | ACTGG | 2 cut(s) | 85, 653 |
| BseRI | GAGGAG | 1 cut(s) | 903 |
| BseSI | GKGCMC | 1 cut(s) | 656 |
| BseXI | GCAGC | 3 cut(s) | 7, 10, 13 |
| Bsh1236I | CGCG | 1 cut(s) | 1010 |
| BshFI | GGCC | 4 cut(s) | 277, 399, 565, 1220 |
| BsiSI | CCGG | 2 cut(s) | 1197, 1217 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 80, 649, 785, 1261 |
| BsmAI | GTCTC | 2 cut(s) | 120, 1138 |
| BsmI | GAATGC | 1 cut(s) | 1112 |
| BsnI | GGCC | 4 cut(s) | 277, 399, 565, 1220 |
| Bso31I | GGTCTC | 1 cut(s) | 1138 |
| Bsp1286I | GDGCHC | 1 cut(s) | 656 |
| Bsp143I | GATC | 9 cut(s) | 228, 243, 507, 634, 820, 967, 1028, 1042, 1051 |
| BspACI | CCGC | 4 cut(s) | 74, 274, 848, 908 |
| BspANI | GGCC | 4 cut(s) | 277, 399, 565, 1220 |
| BspCNI | CTCAG | 3 cut(s) | 580, 846, 1134 |
| BspFNI | CGCG | 1 cut(s) | 1010 |
| BspLI | GGNNCC | 1 cut(s) | 662 |
| BspMI | ACCTGC | 1 cut(s) | 1005 |
| BspPI | GGATC | 2 cut(s) | 1046, 1050 |
| BspTNI | GGTCTC | 1 cut(s) | 1138 |
| BsrDI | GCAATG | 1 cut(s) | 1176 |
| BsrFI | RCCGGY | 1 cut(s) | 1216 |
| BsrI | ACTGG | 2 cut(s) | 85, 653 |
| BssAI | RCCGGY | 1 cut(s) | 1216 |
| BssECI | CCNNGG | 1 cut(s) | 779 |
| BssMI | GATC | 9 cut(s) | 228, 243, 507, 634, 820, 967, 1028, 1042, 1051 |
| BssT1I | CCWWGG | 1 cut(s) | 779 |
| Bst4CI | ACNGT | 1 cut(s) | 147 |
| Bst6I | CTCTTC | 1 cut(s) | 234 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 17, 997 |
| BstC8I | GCNNGC | 4 cut(s) | 279, 748, 801, 1098 |
| BstDEI | CTNAG | 7 cut(s) | 138, 308, 567, 719, 833, 971, 1142 |
| BstF5I | GGATG | 2 cut(s) | 554, 1093 |
| BstFNI | CGCG | 1 cut(s) | 1010 |
| BstKTI | GATC | 9 cut(s) | 231, 246, 510, 637, 823, 970, 1031, 1045, 1054 |
| BstMAI | GTCTC | 2 cut(s) | 120, 1138 |
| BstMBI | GATC | 9 cut(s) | 228, 243, 507, 634, 820, 967, 1028, 1042, 1051 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 17, 26, 997 |
| BstNSI | RCATGY | 1 cut(s) | 1182 |
| BstSFI | CTRYAG | 2 cut(s) | 150, 406 |
| BstSLI | GKGCMC | 1 cut(s) | 656 |
| BstUI | CGCG | 1 cut(s) | 1010 |
| BstV1I | GCAGC | 3 cut(s) | 7, 10, 13 |
| BstV2I | GAAGAC | 1 cut(s) | 1240 |
| BstX2I | RGATCY | 2 cut(s) | 967, 1042 |
| BstYI | RGATCY | 2 cut(s) | 967, 1042 |
| BsuI | GTATCC | 1 cut(s) | 1258 |
| BsuRI | GGCC | 4 cut(s) | 277, 399, 565, 1220 |
| BtsCI | GGATG | 2 cut(s) | 554, 1093 |
| BveI | ACCTGC | 1 cut(s) | 1005 |
| Cac8I | GCNNGC | 4 cut(s) | 279, 748, 801, 1098 |
| CaiI | CAGNNNCTG | 4 cut(s) | 17, 643, 872, 997 |
| Cfr10I | RCCGGY | 1 cut(s) | 1216 |
| Cfr13I | GGNCC | 3 cut(s) | 398, 563, 610 |
| CseI | GACGC | 3 cut(s) | 299, 505, 1245 |
| Csp6I | GTAC | 2 cut(s) | 837, 994 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1089, 1124 |
| CviAII | CATG | 6 cut(s) | 65, 592, 704, 1133, 1153, 1179 |
| CviQI | GTAC | 2 cut(s) | 837, 994 |
| DdeI | CTNAG | 7 cut(s) | 138, 308, 567, 719, 833, 971, 1142 |
| DpnI | GATC | 9 cut(s) | 230, 245, 509, 636, 822, 969, 1030, 1044, 1053 |
| DpnII | GATC | 9 cut(s) | 228, 243, 507, 634, 820, 967, 1028, 1042, 1051 |
| EaeI | YGGCCR | 1 cut(s) | 275 |
| Eam1104I | CTCTTC | 1 cut(s) | 234 |
| EarI | CTCTTC | 1 cut(s) | 234 |
| EciI | GGCGGA | 1 cut(s) | 923 |
| Eco130I | CCWWGG | 1 cut(s) | 779 |
| Eco31I | GGTCTC | 1 cut(s) | 1138 |
| Eco32I | GATATC | 1 cut(s) | 739 |
| Eco47I | GGWCC | 1 cut(s) | 610 |
| Eco57I | CTGAAG | 2 cut(s) | 837, 1109 |
| EcoRV | GATATC | 1 cut(s) | 739 |
| EcoT14I | CCWWGG | 1 cut(s) | 779 |
| ErhI | CCWWGG | 1 cut(s) | 779 |
| FaeI | CATG | 6 cut(s) | 68, 595, 707, 1136, 1156, 1182 |
| FatI | CATG | 6 cut(s) | 64, 591, 703, 1132, 1152, 1178 |
| FauI | CCCGC | 1 cut(s) | 841 |
| FbaI | TGATCA | 2 cut(s) | 507, 634 |
| FblI | GTMKAC | 1 cut(s) | 149 |
| Fnu4HI | GCNGC | 5 cut(s) | 21, 24, 27, 74, 275 |
| FokI | GGATG | 2 cut(s) | 561, 1100 |
| Fsp4HI | GCNGC | 5 cut(s) | 21, 24, 27, 74, 275 |
| FspBI | CTAG | 6 cut(s) | 513, 657, 684, 804, 857, 977 |
| GluI | GCNGC | 5 cut(s) | 21, 24, 27, 74, 275 |
| GsuI | CTGGAG | 1 cut(s) | 102 |
| HaeIII | GGCC | 4 cut(s) | 277, 399, 565, 1220 |
| HapII | CCGG | 2 cut(s) | 1197, 1217 |
| HgaI | GACGC | 3 cut(s) | 299, 505, 1245 |
| Hin1II | CATG | 6 cut(s) | 68, 595, 707, 1136, 1156, 1182 |
| HincII | GTYRAC | 1 cut(s) | 1176 |
| HindII | GTYRAC | 1 cut(s) | 1176 |
| HinfI | GANTC | 9 cut(s) | 4, 68, 264, 303, 393, 573, 678, 710, 1304 |
| HpaI | GTTAAC | 1 cut(s) | 1176 |
| HpaII | CCGG | 2 cut(s) | 1197, 1217 |
| HphI | GGTGA | 1 cut(s) | 1048 |
| Hpy166II | GTNNAC | 2 cut(s) | 150, 1176 |
| Hpy188III | TCNNGA | 5 cut(s) | 247, 458, 643, 664, 761 |
| Hpy8I | GTNNAC | 2 cut(s) | 150, 1176 |
| HpyAV | CCTTC | 5 cut(s) | 78, 340, 1064, 1133, 1296 |
| HpyCH4III | ACNGT | 1 cut(s) | 147 |
| HpyCH4V | TGCA | 6 cut(s) | 494, 991, 1063, 1096, 1136, 1182 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 17, 26, 997 |
| HpyF3I | CTNAG | 7 cut(s) | 138, 308, 567, 719, 833, 971, 1142 |
| Hsp92II | CATG | 6 cut(s) | 68, 595, 707, 1136, 1156, 1182 |
| Ksp22I | TGATCA | 2 cut(s) | 507, 634 |
| KspAI | GTTAAC | 1 cut(s) | 1176 |
| Kzo9I | GATC | 9 cut(s) | 228, 243, 507, 634, 820, 967, 1028, 1042, 1051 |
| Lsp1109I | GCAGC | 3 cut(s) | 7, 10, 13 |
| LweI | GCATC | 2 cut(s) | 290, 989 |
| MaeI | CTAG | 6 cut(s) | 513, 657, 684, 804, 857, 977 |
| MaeIII | GTNAC | 2 cut(s) | 141, 690 |
| MalI | GATC | 9 cut(s) | 230, 245, 509, 636, 822, 969, 1030, 1044, 1053 |
| MboI | GATC | 9 cut(s) | 228, 243, 507, 634, 820, 967, 1028, 1042, 1051 |
| MboII | GAAGA | 6 cut(s) | 251, 355, 749, 802, 830, 1245 |
| MflI | RGATCY | 2 cut(s) | 967, 1042 |
| MhlI | GDGCHC | 1 cut(s) | 656 |
| MluCI | AATT | 9 cut(s) | 93, 204, 416, 669, 723, 770, 893, 945, 1321 |
| MluI | ACGCGT | 1 cut(s) | 1008 |
| MlyI | GAGTC | 3 cut(s) | 62, 273, 567 |
| MmeI | TCCRAC | 3 cut(s) | 526, 544, 655 |
| MroXI | GAANNNNTTC | 1 cut(s) | 771 |
| MseI | TTAA | 5 cut(s) | 36, 419, 444, 1175, 1324 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1117 |
| MspI | CCGG | 2 cut(s) | 1197, 1217 |
| Mva1269I | GAATGC | 1 cut(s) | 1112 |
| MvnI | CGCG | 1 cut(s) | 1010 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 17, 26, 997 |
| NdeII | GATC | 9 cut(s) | 228, 243, 507, 634, 820, 967, 1028, 1042, 1051 |
| NlaIII | CATG | 6 cut(s) | 68, 595, 707, 1136, 1156, 1182 |
| NlaIV | GGNNCC | 1 cut(s) | 662 |
| NmuCI | GTSAC | 1 cut(s) | 141 |
| NspI | RCATGY | 1 cut(s) | 1182 |
| PctI | GAATGC | 1 cut(s) | 1112 |
| PdmI | GAANNNNTTC | 1 cut(s) | 771 |
| PfeI | GAWTC | 6 cut(s) | 4, 303, 393, 678, 710, 1304 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 785 |
| PkrI | GCNGC | 5 cut(s) | 22, 25, 28, 75, 276 |
| PleI | GAGTC | 3 cut(s) | 62, 272, 567 |
| PpsI | GAGTC | 3 cut(s) | 62, 272, 567 |
| PsiI | TTATAA | 1 cut(s) | 203 |
| PspN4I | GGNNCC | 1 cut(s) | 662 |
| PspPI | GGNCC | 3 cut(s) | 398, 563, 610 |
| PstNI | CAGNNNCTG | 4 cut(s) | 17, 643, 872, 997 |
| PsuI | RGATCY | 2 cut(s) | 967, 1042 |
| RsaI | GTAC | 2 cut(s) | 838, 995 |
| RsaNI | GTAC | 2 cut(s) | 837, 994 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1117 |
| SaqAI | TTAA | 5 cut(s) | 36, 419, 444, 1175, 1324 |
| SatI | GCNGC | 5 cut(s) | 21, 24, 27, 74, 275 |
| Sau3AI | GATC | 9 cut(s) | 228, 243, 507, 634, 820, 967, 1028, 1042, 1051 |
| Sau96I | GGNCC | 3 cut(s) | 398, 563, 610 |
| SchI | GAGTC | 3 cut(s) | 62, 273, 567 |
| SduI | GDGCHC | 1 cut(s) | 656 |
| SfaNI | GCATC | 2 cut(s) | 290, 989 |
| SfcI | CTRYAG | 2 cut(s) | 150, 406 |
| SinI | GGWCC | 1 cut(s) | 610 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1117 |
| Sse9I | AATT | 9 cut(s) | 93, 204, 416, 669, 723, 770, 893, 945, 1321 |
| SsiI | CCGC | 4 cut(s) | 74, 274, 848, 908 |
| SspMI | CTAG | 6 cut(s) | 513, 657, 684, 804, 857, 977 |
| StyI | CCWWGG | 1 cut(s) | 779 |
| TaaI | ACNGT | 1 cut(s) | 147 |
| TaqI | TCGA | 2 cut(s) | 284, 762 |
| TasI | AATT | 9 cut(s) | 93, 204, 416, 669, 723, 770, 893, 945, 1321 |
| TatI | WGTACW | 1 cut(s) | 836 |
| TauI | GCSGC | 2 cut(s) | 76, 277 |
| TfiI | GAWTC | 6 cut(s) | 4, 303, 393, 678, 710, 1304 |
| Tru1I | TTAA | 5 cut(s) | 36, 419, 444, 1175, 1324 |
| Tru9I | TTAA | 5 cut(s) | 36, 419, 444, 1175, 1324 |
| TseFI | GTSAC | 1 cut(s) | 141 |
| TseI | GCWGC | 3 cut(s) | 20, 23, 26 |
| Tsp45I | GTSAC | 1 cut(s) | 141 |
| TspDTI | ATGAA | 6 cut(s) | 17, 353, 600, 608, 780, 1263 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 785 |
| VpaK11BI | GGWCC | 1 cut(s) | 610 |
| XapI | RAATTY | 1 cut(s) | 770 |
| XceI | RCATGY | 1 cut(s) | 1182 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 620, 671 |
| XmiI | GTMKAC | 1 cut(s) | 149 |
| XmnI | GAANNNNTTC | 1 cut(s) | 771 |
| XspI | CTAG | 6 cut(s) | 513, 657, 684, 804, 857, 977 |