source UniProtKB
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1208 |
| Acc36I | ACCTGC | 1 cut(s) | 1208 |
| AccB1I | GGYRCC | 1 cut(s) | 680 |
| AclWI | GGATC | 3 cut(s) | 858, 1106, 1154 |
| AcsI | RAATTY | 3 cut(s) | 345, 386, 1084 |
| AcuI | CTGAAG | 1 cut(s) | 41 |
| AfaI | GTAC | 6 cut(s) | 485, 500, 643, 801, 1129, 1191 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 32, 495, 623, 677 |
| AgsI | TTSAA | 8 cut(s) | 301, 350, 429, 514, 841, 875, 1090, 1247 |
| AjiI | CACGTC | 1 cut(s) | 663 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 606, 638 |
| AluBI | AGCT | 7 cut(s) | 124, 353, 449, 454, 718, 1002, 1261 |
| AluI | AGCT | 7 cut(s) | 124, 353, 449, 454, 718, 1002, 1261 |
| Alw26I | GTCTC | 2 cut(s) | 296, 919 |
| AlwI | GGATC | 3 cut(s) | 858, 1106, 1154 |
| AoxI | GGCC | 3 cut(s) | 303, 460, 758 |
| ApeKI | GCWGC | 1 cut(s) | 715 |
| ApoI | RAATTY | 3 cut(s) | 345, 386, 1084 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 389 |
| AspS9I | GGNCC | 3 cut(s) | 461, 759, 785 |
| AsuC2I | CCSGG | 1 cut(s) | 938 |
| AsuHPI | GGTGA | 3 cut(s) | 1232, 1319, 1349 |
| AsuII | TTCGAA | 1 cut(s) | 492 |
| AsuNHI | GCTAGC | 1 cut(s) | 718 |
| AvaII | GGWCC | 1 cut(s) | 785 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1119, 1152 |
| BanI | GGYRCC | 1 cut(s) | 680 |
| BbvI | GCAGC | 1 cut(s) | 727 |
| BccI | CCATC | 8 cut(s) | 228, 441, 471, 546, 640, 776, 1298, 1328 |
| BceAI | ACGGC | 2 cut(s) | 194, 969 |
| BcnI | CCSGG | 1 cut(s) | 938 |
| BcoDI | GTCTC | 2 cut(s) | 296, 919 |
| BfaI | CTAG | 7 cut(s) | 80, 636, 719, 789, 911, 918, 1317 |
| BfmI | CTRYAG | 1 cut(s) | 1293 |
| BfuAI | ACCTGC | 1 cut(s) | 1208 |
| BisI | GCNGC | 1 cut(s) | 716 |
| BlpI | GCTNAGC | 1 cut(s) | 450 |
| BlsI | GCNGC | 1 cut(s) | 717 |
| Bme1390I | CCNGG | 1 cut(s) | 938 |
| Bme18I | GGWCC | 1 cut(s) | 785 |
| BmgBI | CACGTC | 1 cut(s) | 663 |
| BmgT120I | GGNCC | 3 cut(s) | 461, 759, 785 |
| BmiI | GGNNCC | 3 cut(s) | 530, 682, 1011 |
| BmrFI | CCNGG | 1 cut(s) | 938 |
| BmsI | GCATC | 1 cut(s) | 324 |
| BmtI | GCTAGC | 1 cut(s) | 722 |
| BoxI | GACNNNNGTC | 1 cut(s) | 673 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 450 |
| Bpu14I | TTCGAA | 1 cut(s) | 492 |
| BpuEI | CTTGAG | 1 cut(s) | 1271 |
| BpuMI | CCSGG | 1 cut(s) | 938 |
| BsaJI | CCNNGG | 2 cut(s) | 165, 846 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1264, 1294 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 32, 495, 623, 677 |
| Bse1I | ACTGG | 1 cut(s) | 263 |
| Bse3DI | GCAATG | 3 cut(s) | 234, 552, 912 |
| BseDI | CCNNGG | 2 cut(s) | 165, 846 |
| BseGI | GGATG | 4 cut(s) | 175, 486, 538, 1339 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 32, 495, 623, 677 |
| BseMI | GCAATG | 3 cut(s) | 234, 552, 912 |
| BseMII | CTCAG | 1 cut(s) | 441 |
| BseNI | ACTGG | 1 cut(s) | 263 |
| BseRI | GAGGAG | 1 cut(s) | 1244 |
| BseXI | GCAGC | 1 cut(s) | 727 |
| BsgI | GTGCAG | 1 cut(s) | 685 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1287 |
| BshFI | GGCC | 3 cut(s) | 305, 462, 760 |
| BshNI | GGYRCC | 1 cut(s) | 680 |
| BsiEI | CGRYCG | 1 cut(s) | 1287 |
| BsiSI | CCGG | 1 cut(s) | 938 |
| BsiWI | CGTACG | 1 cut(s) | 1189 |
| BslFI | GGGAC | 1 cut(s) | 673 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 32, 495, 623, 677 |
| BsmAI | GTCTC | 2 cut(s) | 296, 919 |
| BsmFI | GGGAC | 1 cut(s) | 673 |
| BsnI | GGCC | 3 cut(s) | 305, 462, 760 |
| Bsp119I | TTCGAA | 1 cut(s) | 492 |
| Bsp143I | GATC | 8 cut(s) | 130, 172, 355, 690, 850, 1111, 1159, 1284 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 450 |
| Bsp19I | CCATGG | 1 cut(s) | 165 |
| BspANI | GGCC | 3 cut(s) | 305, 462, 760 |
| BspCNI | CTCAG | 1 cut(s) | 442 |
| BspHI | TCATGA | 1 cut(s) | 414 |
| BspLI | GGNNCC | 3 cut(s) | 530, 682, 1011 |
| BspMAI | CTGCAG | 1 cut(s) | 1297 |
| BspMI | ACCTGC | 1 cut(s) | 1208 |
| BspOI | GCTAGC | 1 cut(s) | 722 |
| BspPI | GGATC | 3 cut(s) | 858, 1106, 1154 |
| BspT104I | TTCGAA | 1 cut(s) | 492 |
| BspT107I | GGYRCC | 1 cut(s) | 680 |
| BsrDI | GCAATG | 3 cut(s) | 234, 552, 912 |
| BsrI | ACTGG | 1 cut(s) | 263 |
| BssECI | CCNNGG | 2 cut(s) | 165, 846 |
| BssMI | GATC | 8 cut(s) | 130, 172, 355, 690, 850, 1111, 1159, 1284 |
| BssT1I | CCWWGG | 2 cut(s) | 165, 846 |
| Bst4CI | ACNGT | 7 cut(s) | 97, 214, 370, 399, 533, 622, 1025 |
| Bst6I | CTCTTC | 2 cut(s) | 146, 299 |
| BstBI | TTCGAA | 1 cut(s) | 492 |
| BstC8I | GCNNGC | 2 cut(s) | 126, 720 |
| BstDEI | CTNAG | 1 cut(s) | 450 |
| BstDSI | CCRYGG | 1 cut(s) | 165 |
| BstEII | GGTNACC | 1 cut(s) | 1307 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 493, 675 |
| BstF5I | GGATG | 4 cut(s) | 175, 486, 538, 1339 |
| BstKTI | GATC | 8 cut(s) | 133, 175, 358, 693, 853, 1114, 1162, 1287 |
| BstMAI | GTCTC | 2 cut(s) | 296, 919 |
| BstMBI | GATC | 8 cut(s) | 130, 172, 355, 690, 850, 1111, 1159, 1284 |
| BstMCI | CGRYCG | 1 cut(s) | 1287 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 578 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 673 |
| BstPI | GGTNACC | 1 cut(s) | 1307 |
| BstSCI | CCNGG | 1 cut(s) | 936 |
| BstSFI | CTRYAG | 1 cut(s) | 1293 |
| BstV1I | GCAGC | 1 cut(s) | 727 |
| BsuRI | GGCC | 3 cut(s) | 305, 462, 760 |
| BtgI | CCRYGG | 1 cut(s) | 165 |
| BtgZI | GCGATG | 1 cut(s) | 553 |
| BtrI | CACGTC | 1 cut(s) | 663 |
| BtsCI | GGATG | 4 cut(s) | 175, 486, 538, 1339 |
| BtsI | GCAGTG | 2 cut(s) | 886, 1290 |
| BtsIMutI | CAGTG | 3 cut(s) | 817, 886, 1290 |
| BveI | ACCTGC | 1 cut(s) | 1208 |
| Cac8I | GCNNGC | 2 cut(s) | 126, 720 |
| CciI | TCATGA | 1 cut(s) | 414 |
| Cfr13I | GGNCC | 3 cut(s) | 461, 759, 785 |
| Csp6I | GTAC | 6 cut(s) | 484, 499, 642, 800, 1128, 1190 |
| CviAII | CATG | 7 cut(s) | 76, 118, 166, 200, 415, 502, 1214 |
| CviQI | GTAC | 6 cut(s) | 484, 499, 642, 800, 1128, 1190 |
| DdeI | CTNAG | 1 cut(s) | 450 |
| DpnI | GATC | 8 cut(s) | 132, 174, 357, 692, 852, 1113, 1161, 1286 |
| DpnII | GATC | 8 cut(s) | 130, 172, 355, 690, 850, 1111, 1159, 1284 |
| Eam1104I | CTCTTC | 2 cut(s) | 146, 299 |
| EarI | CTCTTC | 2 cut(s) | 146, 299 |
| Eco130I | CCWWGG | 2 cut(s) | 165, 846 |
| Eco32I | GATATC | 1 cut(s) | 412 |
| Eco47I | GGWCC | 1 cut(s) | 785 |
| Eco57I | CTGAAG | 1 cut(s) | 41 |
| Eco91I | GGTNACC | 1 cut(s) | 1307 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 493, 675 |
| EcoO109I | RGGNCCY | 1 cut(s) | 759 |
| EcoO65I | GGTNACC | 1 cut(s) | 1307 |
| EcoRV | GATATC | 1 cut(s) | 412 |
| EcoT14I | CCWWGG | 2 cut(s) | 165, 846 |
| ErhI | CCWWGG | 2 cut(s) | 165, 846 |
| FaeI | CATG | 7 cut(s) | 79, 121, 169, 203, 418, 505, 1217 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 342, 374 |
| FaqI | GGGAC | 1 cut(s) | 673 |
| FatI | CATG | 7 cut(s) | 75, 117, 165, 199, 414, 501, 1213 |
| FauNDI | CATATG | 1 cut(s) | 308 |
| Fnu4HI | GCNGC | 1 cut(s) | 716 |
| FokI | GGATG | 4 cut(s) | 182, 493, 525, 1346 |
| Fsp4HI | GCNGC | 1 cut(s) | 716 |
| FspBI | CTAG | 7 cut(s) | 80, 636, 719, 789, 911, 918, 1317 |
| GluI | GCNGC | 1 cut(s) | 716 |
| HaeIII | GGCC | 3 cut(s) | 305, 462, 760 |
| HapII | CCGG | 1 cut(s) | 938 |
| Hin1II | CATG | 7 cut(s) | 79, 121, 169, 203, 418, 505, 1217 |
| HincII | GTYRAC | 1 cut(s) | 1036 |
| HindII | GTYRAC | 1 cut(s) | 1036 |
| HindIII | AAGCTT | 1 cut(s) | 122 |
| HinfI | GANTC | 5 cut(s) | 70, 418, 560, 966, 1239 |
| HpaII | CCGG | 1 cut(s) | 938 |
| HphI | GGTGA | 3 cut(s) | 1232, 1319, 1349 |
| Hpy166II | GTNNAC | 1 cut(s) | 1036 |
| Hpy188I | TCNGA | 4 cut(s) | 21, 649, 796, 1284 |
| Hpy188III | TCNNGA | 4 cut(s) | 415, 542, 854, 1199 |
| Hpy8I | GTNNAC | 1 cut(s) | 1036 |
| HpyAV | CCTTC | 6 cut(s) | 412, 499, 539, 649, 919, 1337 |
| HpyCH4III | ACNGT | 7 cut(s) | 97, 214, 370, 399, 533, 622, 1025 |
| HpyCH4IV | ACGT | 2 cut(s) | 601, 662 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 578 |
| HpyF3I | CTNAG | 1 cut(s) | 450 |
| HpySE526I | ACGT | 2 cut(s) | 601, 662 |
| Hsp92II | CATG | 7 cut(s) | 79, 121, 169, 203, 418, 505, 1217 |
| Kzo9I | GATC | 8 cut(s) | 130, 172, 355, 690, 850, 1111, 1159, 1284 |
| LmnI | GCTCC | 1 cut(s) | 234 |
| LpnPI | CCDG | 9 cut(s) | 276, 555, 663, 665, 951, 1026, 1203, 1212, 1316 |
| Lsp1109I | GCAGC | 1 cut(s) | 727 |
| LweI | GCATC | 1 cut(s) | 324 |
| MaeI | CTAG | 7 cut(s) | 80, 636, 719, 789, 911, 918, 1317 |
| MaeII | ACGT | 2 cut(s) | 601, 662 |
| MaeIII | GTNAC | 3 cut(s) | 533, 1148, 1307 |
| MalI | GATC | 8 cut(s) | 132, 174, 357, 692, 852, 1113, 1161, 1286 |
| MboI | GATC | 8 cut(s) | 130, 172, 355, 690, 850, 1111, 1159, 1284 |
| MboII | GAAGA | 8 cut(s) | 95, 163, 286, 289, 382, 397, 587, 1352 |
| MluCI | AATT | 9 cut(s) | 30, 50, 240, 345, 386, 835, 1084, 1174, 1321 |
| MlyI | GAGTC | 2 cut(s) | 975, 1248 |
| MmeI | TCCRAC | 3 cut(s) | 47, 604, 1216 |
| MnlI | CCTC | 5 cut(s) | 227, 1173, 1222, 1225, 1290 |
| MroXI | GAANNNNTTC | 1 cut(s) | 389 |
| MseI | TTAA | 7 cut(s) | 282, 834, 942, 972, 1053, 1107, 1167 |
| MslI | CAYNNNNRTG | 3 cut(s) | 729, 1107, 1218 |
| MspI | CCGG | 1 cut(s) | 938 |
| MspR9I | CCNGG | 1 cut(s) | 938 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 578 |
| NciI | CCSGG | 1 cut(s) | 938 |
| NcoI | CCATGG | 1 cut(s) | 165 |
| NdeI | CATATG | 1 cut(s) | 308 |
| NdeII | GATC | 8 cut(s) | 130, 172, 355, 690, 850, 1111, 1159, 1284 |
| NheI | GCTAGC | 1 cut(s) | 718 |
| NlaIII | CATG | 7 cut(s) | 79, 121, 169, 203, 418, 505, 1217 |
| NlaIV | GGNNCC | 3 cut(s) | 530, 682, 1011 |
| NmuCI | GTSAC | 1 cut(s) | 1307 |
| NspV | TTCGAA | 1 cut(s) | 492 |
| PagI | TCATGA | 1 cut(s) | 414 |
| PaqCI | CACCTGC | 1 cut(s) | 1208 |
| PdmI | GAANNNNTTC | 1 cut(s) | 389 |
| PfeI | GAWTC | 3 cut(s) | 70, 418, 560 |
| Pfl23II | CGTACG | 1 cut(s) | 1189 |
| PkrI | GCNGC | 1 cut(s) | 717 |
| Ple19I | CGATCG | 1 cut(s) | 1287 |
| PleI | GAGTC | 2 cut(s) | 974, 1247 |
| PpsI | GAGTC | 2 cut(s) | 974, 1247 |
| PshAI | GACNNNNGTC | 1 cut(s) | 673 |
| PspEI | GGTNACC | 1 cut(s) | 1307 |
| PspLI | CGTACG | 1 cut(s) | 1189 |
| PspN4I | GGNNCC | 3 cut(s) | 530, 682, 1011 |
| PspPI | GGNCC | 3 cut(s) | 461, 759, 785 |
| PstI | CTGCAG | 1 cut(s) | 1297 |
| PvuI | CGATCG | 1 cut(s) | 1287 |
| RsaI | GTAC | 6 cut(s) | 485, 500, 643, 801, 1129, 1191 |
| RsaNI | GTAC | 6 cut(s) | 484, 499, 642, 800, 1128, 1190 |
| RseI | CAYNNNNRTG | 3 cut(s) | 729, 1107, 1218 |
| SaqAI | TTAA | 7 cut(s) | 282, 834, 942, 972, 1053, 1107, 1167 |
| SatI | GCNGC | 1 cut(s) | 716 |
| Sau3AI | GATC | 8 cut(s) | 130, 172, 355, 690, 850, 1111, 1159, 1284 |
| Sau96I | GGNCC | 3 cut(s) | 461, 759, 785 |
| SchI | GAGTC | 2 cut(s) | 975, 1248 |
| ScrFI | CCNGG | 1 cut(s) | 938 |
| SfaNI | GCATC | 1 cut(s) | 324 |
| SfcI | CTRYAG | 1 cut(s) | 1293 |
| SfuI | TTCGAA | 1 cut(s) | 492 |
| SinI | GGWCC | 1 cut(s) | 785 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 729, 1107, 1218 |
| SmlI | CTYRAG | 1 cut(s) | 1250 |
| SmoI | CTYRAG | 1 cut(s) | 1250 |
| Sse9I | AATT | 9 cut(s) | 30, 50, 240, 345, 386, 835, 1084, 1174, 1321 |
| SspMI | CTAG | 7 cut(s) | 80, 636, 719, 789, 911, 918, 1317 |
| StyD4I | CCNGG | 1 cut(s) | 936 |
| StyI | CCWWGG | 2 cut(s) | 165, 846 |
| TaaI | ACNGT | 7 cut(s) | 97, 214, 370, 399, 533, 622, 1025 |
| TaiI | ACGT | 2 cut(s) | 604, 665 |
| TaqI | TCGA | 5 cut(s) | 363, 408, 492, 865, 1287 |
| TasI | AATT | 9 cut(s) | 30, 50, 240, 345, 386, 835, 1084, 1174, 1321 |
| TatI | WGTACW | 1 cut(s) | 641 |
| TfiI | GAWTC | 3 cut(s) | 70, 418, 560 |
| Tru1I | TTAA | 7 cut(s) | 282, 834, 942, 972, 1053, 1107, 1167 |
| Tru9I | TTAA | 7 cut(s) | 282, 834, 942, 972, 1053, 1107, 1167 |
| TscAI | CASTG | 3 cut(s) | 817, 886, 1297 |
| TseFI | GTSAC | 1 cut(s) | 1307 |
| TseI | GCWGC | 1 cut(s) | 715 |
| Tsp45I | GTSAC | 1 cut(s) | 1307 |
| TspDTI | ATGAA | 6 cut(s) | 134, 206, 410, 518, 765, 879 |
| TspRI | CASTG | 3 cut(s) | 817, 886, 1297 |
| VpaK11BI | GGWCC | 1 cut(s) | 785 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 493, 675 |
| XapI | RAATTY | 3 cut(s) | 345, 386, 1084 |
| XmnI | GAANNNNTTC | 1 cut(s) | 389 |
| XspI | CTAG | 7 cut(s) | 80, 636, 719, 789, 911, 918, 1317 |