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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1439 |
| Acc36I | ACCTGC | 1 cut(s) | 1439 |
| AccB1I | GGYRCC | 1 cut(s) | 755 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 20 |
| AccI | GTMKAC | 1 cut(s) | 115 |
| AciI | CCGC | 1 cut(s) | 1561 |
| AclWI | GGATC | 2 cut(s) | 1089, 1337 |
| AcsI | RAATTY | 3 cut(s) | 642, 880, 1315 |
| AcuI | CTGAAG | 2 cut(s) | 123, 338 |
| AfaI | GTAC | 6 cut(s) | 158, 235, 718, 1032, 1360, 1422 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 20, 95, 267, 329, 752 |
| AflII | CTTAAG | 1 cut(s) | 997 |
| AgsI | TTSAA | 9 cut(s) | 244, 598, 647, 674, 865, 1072, 1106, 1321, 1478 |
| AjiI | CACGTC | 1 cut(s) | 738 |
| AjnI | CCWGG | 1 cut(s) | 250 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 338, 370, 681, 713 |
| AluBI | AGCT | 5 cut(s) | 421, 634, 650, 793, 1233 |
| AluI | AGCT | 5 cut(s) | 421, 634, 650, 793, 1233 |
| Alw26I | GTCTC | 1 cut(s) | 593 |
| AlwI | GGATC | 2 cut(s) | 1089, 1337 |
| AoxI | GGCC | 3 cut(s) | 59, 600, 833 |
| ApeKI | GCWGC | 4 cut(s) | 34, 275, 790, 956 |
| ApoI | RAATTY | 3 cut(s) | 642, 880, 1315 |
| AseI | ATTAAT | 2 cut(s) | 227, 981 |
| AspS9I | GGNCC | 2 cut(s) | 834, 1016 |
| AsuC2I | CCSGG | 1 cut(s) | 1169 |
| AsuHPI | GGTGA | 3 cut(s) | 1463, 1550, 1580 |
| AsuII | TTCGAA | 1 cut(s) | 147 |
| AsuNHI | GCTAGC | 1 cut(s) | 793 |
| AvaII | GGWCC | 1 cut(s) | 1016 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1350, 1383 |
| BanI | GGYRCC | 1 cut(s) | 755 |
| BbvI | GCAGC | 4 cut(s) | 21, 262, 802, 943 |
| BccI | CCATC | 6 cut(s) | 260, 525, 715, 1007, 1529, 1559 |
| BceAI | ACGGC | 1 cut(s) | 491 |
| BciT130I | CCWGG | 1 cut(s) | 252 |
| BciVI | GTATCC | 1 cut(s) | 933 |
| BcnI | CCSGG | 1 cut(s) | 1169 |
| BcoDI | GTCTC | 1 cut(s) | 593 |
| BfaI | CTAG | 6 cut(s) | 377, 711, 794, 1020, 1149, 1548 |
| BfmI | CTRYAG | 1 cut(s) | 1524 |
| BfrI | CTTAAG | 1 cut(s) | 997 |
| BfuAI | ACCTGC | 1 cut(s) | 1439 |
| BfuI | GTATCC | 1 cut(s) | 933 |
| BisI | GCNGC | 4 cut(s) | 35, 276, 791, 957 |
| BlsI | GCNGC | 4 cut(s) | 36, 277, 792, 958 |
| BmcAI | AGTACT | 1 cut(s) | 158 |
| Bme1390I | CCNGG | 2 cut(s) | 252, 1169 |
| Bme18I | GGWCC | 1 cut(s) | 1016 |
| BmgBI | CACGTC | 1 cut(s) | 738 |
| BmgT120I | GGNCC | 2 cut(s) | 834, 1016 |
| BmiI | GGNNCC | 3 cut(s) | 6, 757, 1242 |
| BmrFI | CCNGG | 2 cut(s) | 252, 1169 |
| BmsI | GCATC | 1 cut(s) | 211 |
| BmtI | GCTAGC | 1 cut(s) | 797 |
| BoxI | GACNNNNGTC | 1 cut(s) | 748 |
| Bpu14I | TTCGAA | 1 cut(s) | 147 |
| BpuEI | CTTGAG | 2 cut(s) | 173, 1502 |
| BpuMI | CCSGG | 1 cut(s) | 1169 |
| BsaJI | CCNNGG | 3 cut(s) | 251, 462, 1077 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1495, 1525 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 20, 95, 267, 329, 752 |
| Bse1I | ACTGG | 3 cut(s) | 62, 164, 560 |
| Bse3DI | GCAATG | 3 cut(s) | 303, 531, 1143 |
| BseBI | CCWGG | 1 cut(s) | 252 |
| BseDI | CCNNGG | 3 cut(s) | 251, 462, 1077 |
| BseGI | GGATG | 4 cut(s) | 82, 472, 874, 1570 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 20, 95, 267, 329, 752 |
| BseMI | GCAATG | 3 cut(s) | 303, 531, 1143 |
| BseNI | ACTGG | 3 cut(s) | 62, 164, 560 |
| BseRI | GAGGAG | 1 cut(s) | 1475 |
| BseXI | GCAGC | 4 cut(s) | 21, 262, 802, 943 |
| BseYI | CCCAGC | 1 cut(s) | 959 |
| BsgI | GTGCAG | 1 cut(s) | 760 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1518 |
| BshFI | GGCC | 3 cut(s) | 61, 602, 835 |
| BshNI | GGYRCC | 1 cut(s) | 755 |
| BsiEI | CGRYCG | 1 cut(s) | 1518 |
| BsiSI | CCGG | 1 cut(s) | 1169 |
| BsiWI | CGTACG | 1 cut(s) | 1420 |
| BslFI | GGGAC | 1 cut(s) | 748 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 20, 95, 267, 329, 752 |
| BsmAI | GTCTC | 1 cut(s) | 593 |
| BsmFI | GGGAC | 1 cut(s) | 748 |
| BsnI | GGCC | 3 cut(s) | 61, 602, 835 |
| Bsp119I | TTCGAA | 1 cut(s) | 147 |
| Bsp143I | GATC | 7 cut(s) | 427, 469, 542, 765, 1081, 1342, 1515 |
| Bsp19I | CCATGG | 1 cut(s) | 462 |
| BspACI | CCGC | 1 cut(s) | 1561 |
| BspANI | GGCC | 3 cut(s) | 61, 602, 835 |
| BspLI | GGNNCC | 3 cut(s) | 6, 757, 1242 |
| BspMI | ACCTGC | 1 cut(s) | 1439 |
| BspOI | GCTAGC | 1 cut(s) | 797 |
| BspPI | GGATC | 2 cut(s) | 1089, 1337 |
| BspT104I | TTCGAA | 1 cut(s) | 147 |
| BspT107I | GGYRCC | 1 cut(s) | 755 |
| BspTI | CTTAAG | 1 cut(s) | 997 |
| BsrDI | GCAATG | 3 cut(s) | 303, 531, 1143 |
| BsrI | ACTGG | 3 cut(s) | 62, 164, 560 |
| BssECI | CCNNGG | 3 cut(s) | 251, 462, 1077 |
| BssMI | GATC | 7 cut(s) | 427, 469, 542, 765, 1081, 1342, 1515 |
| BssT1I | CCWWGG | 2 cut(s) | 462, 1077 |
| Bst2UI | CCWGG | 1 cut(s) | 252 |
| Bst4CI | ACNGT | 4 cut(s) | 394, 511, 697, 1256 |
| Bst6I | CTCTTC | 2 cut(s) | 443, 596 |
| BstAFI | CTTAAG | 1 cut(s) | 997 |
| BstBI | TTCGAA | 1 cut(s) | 147 |
| BstC8I | GCNNGC | 1 cut(s) | 795 |
| BstDSI | CCRYGG | 1 cut(s) | 462 |
| BstEII | GGTNACC | 1 cut(s) | 1538 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 750 |
| BstF5I | GGATG | 4 cut(s) | 82, 472, 874, 1570 |
| BstKTI | GATC | 7 cut(s) | 430, 472, 545, 768, 1084, 1345, 1518 |
| BstMAI | GTCTC | 1 cut(s) | 593 |
| BstMBI | GATC | 7 cut(s) | 427, 469, 542, 765, 1081, 1342, 1515 |
| BstMCI | CGRYCG | 1 cut(s) | 1518 |
| BstNI | CCWGG | 1 cut(s) | 252 |
| BstNSI | RCATGY | 1 cut(s) | 872 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 748 |
| BstPI | GGTNACC | 1 cut(s) | 1538 |
| BstSCI | CCNGG | 2 cut(s) | 250, 1167 |
| BstSFI | CTRYAG | 1 cut(s) | 1524 |
| BstV1I | GCAGC | 4 cut(s) | 21, 262, 802, 943 |
| BsuI | GTATCC | 1 cut(s) | 933 |
| BsuRI | GGCC | 3 cut(s) | 61, 602, 835 |
| BtgI | CCRYGG | 1 cut(s) | 462 |
| BtgZI | GCGATG | 1 cut(s) | 234 |
| BtrI | CACGTC | 1 cut(s) | 738 |
| BtsCI | GGATG | 4 cut(s) | 82, 472, 874, 1570 |
| BtsI | GCAGTG | 1 cut(s) | 1117 |
| BtsIMutI | CAGTG | 2 cut(s) | 1048, 1117 |
| BveI | ACCTGC | 1 cut(s) | 1439 |
| Cac8I | GCNNGC | 1 cut(s) | 795 |
| Cfr13I | GGNCC | 2 cut(s) | 834, 1016 |
| Csp6I | GTAC | 6 cut(s) | 157, 234, 717, 1031, 1359, 1421 |
| CviAII | CATG | 8 cut(s) | 26, 120, 373, 415, 463, 497, 869, 1445 |
| CviQI | GTAC | 6 cut(s) | 157, 234, 717, 1031, 1359, 1421 |
| DpnI | GATC | 7 cut(s) | 429, 471, 544, 767, 1083, 1344, 1517 |
| DpnII | GATC | 7 cut(s) | 427, 469, 542, 765, 1081, 1342, 1515 |
| DraI | TTTAAA | 1 cut(s) | 886 |
| Eam1104I | CTCTTC | 2 cut(s) | 443, 596 |
| EarI | CTCTTC | 2 cut(s) | 443, 596 |
| Eco130I | CCWWGG | 2 cut(s) | 462, 1077 |
| Eco47I | GGWCC | 1 cut(s) | 1016 |
| Eco57I | CTGAAG | 2 cut(s) | 123, 338 |
| Eco91I | GGTNACC | 1 cut(s) | 1538 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 750 |
| EcoO109I | RGGNCCY | 1 cut(s) | 834 |
| EcoO65I | GGTNACC | 1 cut(s) | 1538 |
| EcoRII | CCWGG | 1 cut(s) | 250 |
| EcoT14I | CCWWGG | 2 cut(s) | 462, 1077 |
| EcoT22I | ATGCAT | 1 cut(s) | 226 |
| ErhI | CCWWGG | 2 cut(s) | 462, 1077 |
| FaeI | CATG | 8 cut(s) | 29, 123, 376, 418, 466, 500, 872, 1448 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 639, 671 |
| FaqI | GGGAC | 1 cut(s) | 748 |
| FatI | CATG | 8 cut(s) | 25, 119, 372, 414, 462, 496, 868, 1444 |
| FauI | CCCGC | 1 cut(s) | 1554 |
| FauNDI | CATATG | 1 cut(s) | 605 |
| FblI | GTMKAC | 1 cut(s) | 115 |
| Fnu4HI | GCNGC | 4 cut(s) | 35, 276, 791, 957 |
| FokI | GGATG | 4 cut(s) | 69, 479, 861, 1577 |
| Fsp4HI | GCNGC | 4 cut(s) | 35, 276, 791, 957 |
| FspBI | CTAG | 6 cut(s) | 377, 711, 794, 1020, 1149, 1548 |
| GluI | GCNGC | 4 cut(s) | 35, 276, 791, 957 |
| GsaI | CCCAGC | 1 cut(s) | 963 |
| HaeIII | GGCC | 3 cut(s) | 61, 602, 835 |
| HapII | CCGG | 1 cut(s) | 1169 |
| Hin1II | CATG | 8 cut(s) | 29, 123, 376, 418, 466, 500, 872, 1448 |
| HincII | GTYRAC | 1 cut(s) | 1267 |
| HindII | GTYRAC | 1 cut(s) | 1267 |
| HindIII | AAGCTT | 1 cut(s) | 419 |
| HinfI | GANTC | 4 cut(s) | 367, 689, 1197, 1470 |
| HpaII | CCGG | 1 cut(s) | 1169 |
| HphI | GGTGA | 3 cut(s) | 1463, 1550, 1580 |
| Hpy166II | GTNNAC | 3 cut(s) | 23, 116, 1267 |
| Hpy188I | TCNGA | 5 cut(s) | 318, 724, 991, 1027, 1515 |
| Hpy188III | TCNNGA | 4 cut(s) | 188, 205, 1085, 1430 |
| Hpy8I | GTNNAC | 3 cut(s) | 23, 116, 1267 |
| HpyAV | CCTTC | 4 cut(s) | 41, 724, 1150, 1568 |
| HpyCH4III | ACNGT | 4 cut(s) | 394, 511, 697, 1256 |
| HpyCH4IV | ACGT | 2 cut(s) | 676, 737 |
| HpySE526I | ACGT | 2 cut(s) | 676, 737 |
| Hsp92II | CATG | 8 cut(s) | 29, 123, 376, 418, 466, 500, 872, 1448 |
| Kzo9I | GATC | 7 cut(s) | 427, 469, 542, 765, 1081, 1342, 1515 |
| LmnI | GCTCC | 2 cut(s) | 10, 531 |
| Lsp1109I | GCAGC | 4 cut(s) | 21, 262, 802, 943 |
| LweI | GCATC | 1 cut(s) | 211 |
| MaeI | CTAG | 6 cut(s) | 377, 711, 794, 1020, 1149, 1548 |
| MaeII | ACGT | 2 cut(s) | 676, 737 |
| MaeIII | GTNAC | 2 cut(s) | 1379, 1538 |
| MalI | GATC | 7 cut(s) | 429, 471, 544, 767, 1083, 1344, 1517 |
| MboI | GATC | 7 cut(s) | 427, 469, 542, 765, 1081, 1342, 1515 |
| MluCI | AATT | 9 cut(s) | 327, 347, 537, 642, 880, 1066, 1315, 1405, 1552 |
| MlyI | GAGTC | 2 cut(s) | 1206, 1479 |
| MmeI | TCCRAC | 4 cut(s) | 104, 344, 679, 1447 |
| MnlI | CCTC | 8 cut(s) | 22, 90, 96, 524, 1404, 1453, 1456, 1521 |
| Mph1103I | ATGCAT | 1 cut(s) | 226 |
| MslI | CAYNNNNRTG | 3 cut(s) | 804, 1338, 1449 |
| MspCI | CTTAAG | 1 cut(s) | 997 |
| MspI | CCGG | 1 cut(s) | 1169 |
| MspR9I | CCNGG | 2 cut(s) | 252, 1169 |
| MvaI | CCWGG | 1 cut(s) | 252 |
| NciI | CCSGG | 1 cut(s) | 1169 |
| NcoI | CCATGG | 1 cut(s) | 462 |
| NdeI | CATATG | 1 cut(s) | 605 |
| NdeII | GATC | 7 cut(s) | 427, 469, 542, 765, 1081, 1342, 1515 |
| NheI | GCTAGC | 1 cut(s) | 793 |
| NlaIII | CATG | 8 cut(s) | 29, 123, 376, 418, 466, 500, 872, 1448 |
| NlaIV | GGNNCC | 3 cut(s) | 6, 757, 1242 |
| NmuCI | GTSAC | 1 cut(s) | 1538 |
| NsiI | ATGCAT | 1 cut(s) | 226 |
| NspI | RCATGY | 1 cut(s) | 872 |
| NspV | TTCGAA | 1 cut(s) | 147 |
| PaqCI | CACCTGC | 1 cut(s) | 1439 |
| PfeI | GAWTC | 2 cut(s) | 367, 689 |
| Pfl23II | CGTACG | 1 cut(s) | 1420 |
| PflFI | GACNNNGTC | 1 cut(s) | 656 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 20 |
| PkrI | GCNGC | 4 cut(s) | 36, 277, 792, 958 |
| Ple19I | CGATCG | 1 cut(s) | 1518 |
| PleI | GAGTC | 2 cut(s) | 1205, 1478 |
| PpsI | GAGTC | 2 cut(s) | 1205, 1478 |
| PshAI | GACNNNNGTC | 1 cut(s) | 748 |
| PshBI | ATTAAT | 2 cut(s) | 227, 981 |
| Psp6I | CCWGG | 1 cut(s) | 250 |
| PspEI | GGTNACC | 1 cut(s) | 1538 |
| PspFI | CCCAGC | 1 cut(s) | 959 |
| PspGI | CCWGG | 1 cut(s) | 250 |
| PspLI | CGTACG | 1 cut(s) | 1420 |
| PspN4I | GGNNCC | 3 cut(s) | 6, 757, 1242 |
| PspPI | GGNCC | 2 cut(s) | 834, 1016 |
| PsyI | GACNNNGTC | 1 cut(s) | 656 |
| PvuI | CGATCG | 1 cut(s) | 1518 |
| RsaI | GTAC | 6 cut(s) | 158, 235, 718, 1032, 1360, 1422 |
| RsaNI | GTAC | 6 cut(s) | 157, 234, 717, 1031, 1359, 1421 |
| RseI | CAYNNNNRTG | 3 cut(s) | 804, 1338, 1449 |
| SatI | GCNGC | 4 cut(s) | 35, 276, 791, 957 |
| Sau3AI | GATC | 7 cut(s) | 427, 469, 542, 765, 1081, 1342, 1515 |
| Sau96I | GGNCC | 2 cut(s) | 834, 1016 |
| ScaI | AGTACT | 1 cut(s) | 158 |
| SchI | GAGTC | 2 cut(s) | 1206, 1479 |
| ScrFI | CCNGG | 2 cut(s) | 252, 1169 |
| SfaNI | GCATC | 1 cut(s) | 211 |
| SfcI | CTRYAG | 1 cut(s) | 1524 |
| SfuI | TTCGAA | 1 cut(s) | 147 |
| SinI | GGWCC | 1 cut(s) | 1016 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 804, 1338, 1449 |
| SmlI | CTYRAG | 3 cut(s) | 152, 997, 1481 |
| SmoI | CTYRAG | 3 cut(s) | 152, 997, 1481 |
| Sse9I | AATT | 9 cut(s) | 327, 347, 537, 642, 880, 1066, 1315, 1405, 1552 |
| SsiI | CCGC | 1 cut(s) | 1561 |
| SspI | AATATT | 1 cut(s) | 247 |
| SspMI | CTAG | 6 cut(s) | 377, 711, 794, 1020, 1149, 1548 |
| StyD4I | CCNGG | 2 cut(s) | 250, 1167 |
| StyI | CCWWGG | 2 cut(s) | 462, 1077 |
| TaaI | ACNGT | 4 cut(s) | 394, 511, 697, 1256 |
| TaiI | ACGT | 2 cut(s) | 679, 740 |
| TaqI | TCGA | 7 cut(s) | 147, 178, 204, 541, 660, 1096, 1518 |
| TasI | AATT | 9 cut(s) | 327, 347, 537, 642, 880, 1066, 1315, 1405, 1552 |
| TatI | WGTACW | 2 cut(s) | 156, 716 |
| TfiI | GAWTC | 2 cut(s) | 367, 689 |
| TscAI | CASTG | 2 cut(s) | 1048, 1117 |
| TseFI | GTSAC | 1 cut(s) | 1538 |
| TseI | GCWGC | 4 cut(s) | 34, 275, 790, 956 |
| Tsp45I | GTSAC | 1 cut(s) | 1538 |
| TspDTI | ATGAA | 7 cut(s) | 68, 431, 503, 840, 861, 863, 1110 |
| TspRI | CASTG | 2 cut(s) | 1048, 1117 |
| Tth111I | GACNNNGTC | 1 cut(s) | 656 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 20 |
| Vha464I | CTTAAG | 1 cut(s) | 997 |
| VpaK11BI | GGWCC | 1 cut(s) | 1016 |
| VspI | ATTAAT | 2 cut(s) | 227, 981 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 750 |
| XapI | RAATTY | 3 cut(s) | 642, 880, 1315 |
| XceI | RCATGY | 1 cut(s) | 872 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 268 |
| XmiI | GTMKAC | 1 cut(s) | 115 |
| XspI | CTAG | 6 cut(s) | 377, 711, 794, 1020, 1149, 1548 |
| ZrmI | AGTACT | 1 cut(s) | 158 |
| Zsp2I | ATGCAT | 1 cut(s) | 226 |