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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 5 |
| AccB1I | GGYRCC | 1 cut(s) | 2137 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1088 |
| AccBSI | CCGCTC | 1 cut(s) | 1204 |
| AccII | CGCG | 3 cut(s) | 492, 1962, 2250 |
| AccIII | TCCGGA | 1 cut(s) | 34 |
| AclI | AACGTT | 1 cut(s) | 766 |
| AclWI | GGATC | 4 cut(s) | 835, 1694, 1907, 1920 |
| AcoI | YGGCCR | 6 cut(s) | 999, 1288, 1800, 1809, 1890, 2200 |
| AcsI | RAATTY | 4 cut(s) | 191, 233, 1383, 2117 |
| AcuI | CTGAAG | 5 cut(s) | 87, 461, 914, 1062, 2250 |
| AdeI | CACNNNGTG | 1 cut(s) | 486 |
| AfaI | GTAC | 3 cut(s) | 290, 1032, 2327 |
| AfeI | AGCGCT | 1 cut(s) | 1039 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 158, 463, 1088 |
| AflIII | ACRYGT | 1 cut(s) | 1876 |
| AjnI | CCWGG | 2 cut(s) | 1708, 1797 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 2103, 2135 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 2259, 2291 |
| Alw26I | GTCTC | 1 cut(s) | 2246 |
| AlwI | GGATC | 4 cut(s) | 835, 1694, 1907, 1920 |
| Ama87I | CYCGRG | 2 cut(s) | 395, 1784 |
| Aor13HI | TCCGGA | 1 cut(s) | 34 |
| Aor51HI | AGCGCT | 1 cut(s) | 1039 |
| AoxI | GGCC | 8 cut(s) | 999, 1288, 1573, 1800, 1809, 1890, 2063, 2200 |
| ApeKI | GCWGC | 4 cut(s) | 164, 889, 936, 1262 |
| ApoI | RAATTY | 4 cut(s) | 191, 233, 1383, 2117 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 912 |
| AspLEI | GCGC | 4 cut(s) | 6, 492, 1040, 1962 |
| AspS9I | GGNCC | 2 cut(s) | 1733, 1820 |
| AsuHPI | GGTGA | 2 cut(s) | 358, 2192 |
| AsuNHI | GCTAGC | 1 cut(s) | 412 |
| AvaI | CYCGRG | 2 cut(s) | 395, 1784 |
| AvaII | GGWCC | 2 cut(s) | 1733, 1820 |
| BalI | TGGCCA | 4 cut(s) | 1001, 1290, 1892, 2202 |
| BamHI | GGATCC | 1 cut(s) | 1912 |
| BanI | GGYRCC | 1 cut(s) | 2137 |
| BbsI | GAAGAC | 2 cut(s) | 752, 803 |
| BbvCI | CCTCAGC | 1 cut(s) | 1839 |
| BbvI | GCAGC | 4 cut(s) | 176, 876, 948, 1249 |
| BccI | CCATC | 5 cut(s) | 818, 1367, 1943, 2149, 2192 |
| BciT130I | CCWGG | 2 cut(s) | 1710, 1799 |
| BciVI | GTATCC | 1 cut(s) | 960 |
| BcoDI | GTCTC | 1 cut(s) | 2246 |
| BfaI | CTAG | 5 cut(s) | 413, 732, 1943, 2075, 2150 |
| BfmI | CTRYAG | 2 cut(s) | 684, 2043 |
| BfoI | RGCGCY | 1 cut(s) | 1041 |
| BfuI | GTATCC | 1 cut(s) | 960 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1808 |
| BisI | GCNGC | 8 cut(s) | 165, 717, 890, 934, 937, 1218, 1263, 1977 |
| BlsI | GCNGC | 8 cut(s) | 166, 718, 891, 935, 938, 1219, 1264, 1978 |
| BmcAI | AGTACT | 1 cut(s) | 1032 |
| Bme1390I | CCNGG | 2 cut(s) | 1710, 1799 |
| Bme18I | GGWCC | 2 cut(s) | 1733, 1820 |
| BmeT110I | CYCGRG | 2 cut(s) | 395, 1784 |
| BmgT120I | GGNCC | 2 cut(s) | 1733, 1820 |
| BmiI | GGNNCC | 4 cut(s) | 1714, 1914, 2139, 2226 |
| BmrFI | CCNGG | 2 cut(s) | 1710, 1799 |
| BmsI | GCATC | 5 cut(s) | 40, 129, 728, 1292, 1666 |
| BmtI | GCTAGC | 1 cut(s) | 416 |
| BoxI | GACNNNNGTC | 2 cut(s) | 516, 1825 |
| BpiI | GAAGAC | 2 cut(s) | 752, 803 |
| BpmI | CTGGAG | 1 cut(s) | 1065 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1839 |
| BpuEI | CTTGAG | 2 cut(s) | 1288, 1311 |
| BsaBI | GATNNNNATC | 1 cut(s) | 832 |
| BsaI | GGTCTC | 1 cut(s) | 2246 |
| BsaJI | CCNNGG | 1 cut(s) | 1291 |
| BsaWI | WCCGGW | 4 cut(s) | 34, 1720, 1730, 1735 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 980, 1010, 1090, 1120 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 158, 463, 1088 |
| Bse118I | RCCGGY | 3 cut(s) | 1691, 1802, 1811 |
| Bse1I | ACTGG | 2 cut(s) | 1790, 2311 |
| Bse3DI | GCAATG | 5 cut(s) | 45, 436, 1410, 2316, 2328 |
| Bse8I | GATNNNNATC | 1 cut(s) | 832 |
| BseAI | TCCGGA | 1 cut(s) | 34 |
| BseBI | CCWGG | 2 cut(s) | 1710, 1799 |
| BseDI | CCNNGG | 1 cut(s) | 1291 |
| BseGI | GGATG | 5 cut(s) | 31, 120, 390, 1378, 1954 |
| BseJI | GATNNNNATC | 1 cut(s) | 832 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 158, 463, 1088 |
| BseMI | GCAATG | 5 cut(s) | 45, 436, 1410, 2316, 2328 |
| BseMII | CTCAG | 3 cut(s) | 42, 1305, 1830 |
| BseNI | ACTGG | 2 cut(s) | 1790, 2311 |
| BseRI | GAGGAG | 3 cut(s) | 1163, 1331, 1511 |
| BseX3I | CGGCCG | 1 cut(s) | 1809 |
| BseXI | GCAGC | 4 cut(s) | 176, 876, 948, 1249 |
| BseYI | CCCAGC | 1 cut(s) | 1920 |
| BsgI | GTGCAG | 1 cut(s) | 183 |
| Bsh1236I | CGCG | 3 cut(s) | 492, 1962, 2250 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1812 |
| BshFI | GGCC | 8 cut(s) | 1001, 1290, 1575, 1802, 1811, 1892, 2065, 2202 |
| BshNI | GGYRCC | 1 cut(s) | 2137 |
| BsiEI | CGRYCG | 1 cut(s) | 1812 |
| BsiHKCI | CYCGRG | 2 cut(s) | 395, 1784 |
| BsiSI | CCGG | 7 cut(s) | 35, 1692, 1721, 1731, 1736, 1803, 1812 |
| BslFI | GGGAC | 4 cut(s) | 949, 1090, 1453, 1766 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 158, 463, 1088 |
| BsmAI | GTCTC | 1 cut(s) | 2246 |
| BsmFI | GGGAC | 4 cut(s) | 949, 1090, 1453, 1766 |
| BsmI | GAATGC | 2 cut(s) | 1853, 2189 |
| BsnI | GGCC | 8 cut(s) | 1001, 1290, 1575, 1802, 1811, 1892, 2065, 2202 |
| Bso31I | GGTCTC | 1 cut(s) | 2246 |
| BsoBI | CYCGRG | 2 cut(s) | 395, 1784 |
| Bsp13I | TCCGGA | 1 cut(s) | 34 |
| Bsp143I | GATC | 7 cut(s) | 418, 496, 583, 688, 827, 1686, 1912 |
| Bsp68I | TCGCGA | 1 cut(s) | 2250 |
| BspANI | GGCC | 8 cut(s) | 1001, 1290, 1575, 1802, 1811, 1892, 2065, 2202 |
| BspCNI | CTCAG | 3 cut(s) | 43, 1306, 1831 |
| BspEI | TCCGGA | 1 cut(s) | 34 |
| BspFNI | CGCG | 3 cut(s) | 492, 1962, 2250 |
| BspHI | TCATGA | 1 cut(s) | 916 |
| BspLI | GGNNCC | 4 cut(s) | 1714, 1914, 2139, 2226 |
| BspOI | GCTAGC | 1 cut(s) | 416 |
| BspPI | GGATC | 4 cut(s) | 835, 1694, 1907, 1920 |
| BspT107I | GGYRCC | 1 cut(s) | 2137 |
| BspTNI | GGTCTC | 1 cut(s) | 2246 |
| BsrBI | CCGCTC | 1 cut(s) | 1204 |
| BsrDI | GCAATG | 5 cut(s) | 45, 436, 1410, 2316, 2328 |
| BsrFI | RCCGGY | 3 cut(s) | 1691, 1802, 1811 |
| BsrI | ACTGG | 2 cut(s) | 1790, 2311 |
| BssAI | RCCGGY | 3 cut(s) | 1691, 1802, 1811 |
| BssECI | CCNNGG | 1 cut(s) | 1291 |
| BssMI | GATC | 7 cut(s) | 418, 496, 583, 688, 827, 1686, 1912 |
| BssT1I | CCWWGG | 1 cut(s) | 1291 |
| Bst2UI | CCWGG | 2 cut(s) | 1710, 1799 |
| Bst4CI | ACNGT | 4 cut(s) | 635, 1493, 1897, 2032 |
| Bst6I | CTCTTC | 7 cut(s) | 63, 266, 776, 1004, 1120, 2094, 2178 |
| BstC8I | GCNNGC | 7 cut(s) | 201, 215, 372, 414, 1206, 2040, 2290 |
| BstDEI | CTNAG | 8 cut(s) | 51, 483, 777, 993, 1034, 1221, 1314, 1839 |
| BstEII | GGTNACC | 1 cut(s) | 452 |
| BstF5I | GGATG | 5 cut(s) | 31, 120, 390, 1378, 1954 |
| BstFNI | CGCG | 3 cut(s) | 492, 1962, 2250 |
| BstH2I | RGCGCY | 1 cut(s) | 1041 |
| BstHHI | GCGC | 4 cut(s) | 6, 492, 1040, 1962 |
| BstKTI | GATC | 7 cut(s) | 421, 499, 586, 691, 830, 1689, 1915 |
| BstMAI | GTCTC | 1 cut(s) | 2246 |
| BstMBI | GATC | 7 cut(s) | 418, 496, 583, 688, 827, 1686, 1912 |
| BstMCI | CGRYCG | 1 cut(s) | 1812 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 71, 895, 919, 998, 1214, 1226, 1581, 1808, 2135 |
| BstNI | CCWGG | 2 cut(s) | 1710, 1799 |
| BstNSI | RCATGY | 4 cut(s) | 217, 905, 1283, 1880 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 516, 1825 |
| BstPI | GGTNACC | 1 cut(s) | 452 |
| BstSCI | CCNGG | 2 cut(s) | 1708, 1797 |
| BstSFI | CTRYAG | 2 cut(s) | 684, 2043 |
| BstUI | CGCG | 3 cut(s) | 492, 1962, 2250 |
| BstV1I | GCAGC | 4 cut(s) | 176, 876, 948, 1249 |
| BstV2I | GAAGAC | 2 cut(s) | 752, 803 |
| BstX2I | RGATCY | 2 cut(s) | 827, 1912 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1900 |
| BstYI | RGATCY | 2 cut(s) | 827, 1912 |
| BstZI | CGGCCG | 1 cut(s) | 1809 |
| BsuI | GTATCC | 1 cut(s) | 960 |
| BsuRI | GGCC | 8 cut(s) | 1001, 1290, 1575, 1802, 1811, 1892, 2065, 2202 |
| BtgZI | GCGATG | 2 cut(s) | 507, 1632 |
| BtsCI | GGATG | 5 cut(s) | 31, 120, 390, 1378, 1954 |
| BtsIMutI | CAGTG | 5 cut(s) | 225, 1572, 1701, 1893, 2037 |
| BtuMI | TCGCGA | 1 cut(s) | 2250 |
| Cac8I | GCNNGC | 7 cut(s) | 201, 215, 372, 414, 1206, 2040, 2290 |
| CciI | TCATGA | 1 cut(s) | 916 |
| CfoI | GCGC | 4 cut(s) | 6, 492, 1040, 1962 |
| Cfr10I | RCCGGY | 3 cut(s) | 1691, 1802, 1811 |
| Cfr13I | GGNCC | 2 cut(s) | 1733, 1820 |
| CpoI | CGGWCCG | 1 cut(s) | 1733 |
| CsiI | ACCWGGT | 1 cut(s) | 1708 |
| Csp6I | GTAC | 3 cut(s) | 289, 1031, 2326 |
| CspI | CGGWCCG | 1 cut(s) | 1733 |
| CviQI | GTAC | 3 cut(s) | 289, 1031, 2326 |
| DdeI | CTNAG | 8 cut(s) | 51, 483, 777, 993, 1034, 1221, 1314, 1839 |
| DpnI | GATC | 7 cut(s) | 420, 498, 585, 690, 829, 1688, 1914 |
| DpnII | GATC | 7 cut(s) | 418, 496, 583, 688, 827, 1686, 1912 |
| DraIII | CACNNNGTG | 1 cut(s) | 486 |
| EaeI | YGGCCR | 6 cut(s) | 999, 1288, 1800, 1809, 1890, 2200 |
| EagI | CGGCCG | 1 cut(s) | 1809 |
| Eam1104I | CTCTTC | 7 cut(s) | 63, 266, 776, 1004, 1120, 2094, 2178 |
| EarI | CTCTTC | 7 cut(s) | 63, 266, 776, 1004, 1120, 2094, 2178 |
| EclXI | CGGCCG | 1 cut(s) | 1809 |
| Eco130I | CCWWGG | 1 cut(s) | 1291 |
| Eco31I | GGTCTC | 1 cut(s) | 2246 |
| Eco47I | GGWCC | 2 cut(s) | 1733, 1820 |
| Eco47III | AGCGCT | 1 cut(s) | 1039 |
| Eco52I | CGGCCG | 1 cut(s) | 1809 |
| Eco57I | CTGAAG | 5 cut(s) | 87, 461, 914, 1062, 2250 |
| Eco88I | CYCGRG | 2 cut(s) | 395, 1784 |
| Eco91I | GGTNACC | 1 cut(s) | 452 |
| EcoO65I | GGTNACC | 1 cut(s) | 452 |
| EcoRII | CCWGG | 2 cut(s) | 1708, 1797 |
| EcoT14I | CCWWGG | 1 cut(s) | 1291 |
| EcoT22I | ATGCAT | 1 cut(s) | 1285 |
| ErhI | CCWWGG | 1 cut(s) | 1291 |
| FaqI | GGGAC | 4 cut(s) | 949, 1090, 1453, 1766 |
| FauI | CCCGC | 2 cut(s) | 1197, 1553 |
| Fnu4HI | GCNGC | 8 cut(s) | 165, 717, 890, 934, 937, 1218, 1263, 1977 |
| FokI | GGATG | 5 cut(s) | 18, 107, 377, 1385, 1961 |
| Fsp4HI | GCNGC | 8 cut(s) | 165, 717, 890, 934, 937, 1218, 1263, 1977 |
| FspBI | CTAG | 5 cut(s) | 413, 732, 1943, 2075, 2150 |
| FspI | TGCGCA | 1 cut(s) | 5 |
| GlaI | GCGC | 4 cut(s) | 5, 491, 1039, 1961 |
| GluI | GCNGC | 8 cut(s) | 165, 717, 890, 934, 937, 1218, 1263, 1977 |
| GsaI | CCCAGC | 1 cut(s) | 1924 |
| GsuI | CTGGAG | 1 cut(s) | 1065 |
| HaeII | RGCGCY | 1 cut(s) | 1041 |
| HaeIII | GGCC | 8 cut(s) | 1001, 1290, 1575, 1802, 1811, 1892, 2065, 2202 |
| HapII | CCGG | 7 cut(s) | 35, 1692, 1721, 1731, 1736, 1803, 1812 |
| HhaI | GCGC | 4 cut(s) | 6, 492, 1040, 1962 |
| Hin6I | GCGC | 4 cut(s) | 4, 490, 1038, 1960 |
| HinP1I | GCGC | 4 cut(s) | 4, 490, 1038, 1960 |
| HincII | GTYRAC | 1 cut(s) | 1489 |
| HindII | GTYRAC | 1 cut(s) | 1489 |
| HindIII | AAGCTT | 2 cut(s) | 368, 911 |
| HinfI | GANTC | 7 cut(s) | 173, 362, 529, 559, 869, 1078, 1775 |
| HpaI | GTTAAC | 1 cut(s) | 1489 |
| HpaII | CCGG | 7 cut(s) | 35, 1692, 1721, 1731, 1736, 1803, 1812 |
| HphI | GGTGA | 2 cut(s) | 358, 2192 |
| Hpy166II | GTNNAC | 5 cut(s) | 316, 594, 1363, 1489, 1697 |
| Hpy188I | TCNGA | 6 cut(s) | 564, 823, 1389, 1533, 1675, 1717 |
| Hpy188III | TCNNGA | 9 cut(s) | 35, 397, 439, 503, 917, 1060, 1082, 1328, 2249 |
| Hpy8I | GTNNAC | 5 cut(s) | 316, 594, 1363, 1489, 1697 |
| HpyAV | CCTTC | 9 cut(s) | 32, 343, 559, 751, 835, 1037, 1089, 1926, 2290 |
| HpyCH4III | ACNGT | 4 cut(s) | 635, 1493, 1897, 2032 |
| HpyCH4IV | ACGT | 1 cut(s) | 766 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 71, 895, 919, 998, 1214, 1226, 1581, 1808, 2135 |
| HpyF3I | CTNAG | 8 cut(s) | 51, 483, 777, 993, 1034, 1221, 1314, 1839 |
| HpySE526I | ACGT | 1 cut(s) | 766 |
| HspAI | GCGC | 4 cut(s) | 4, 490, 1038, 1960 |
| Kpn2I | TCCGGA | 1 cut(s) | 34 |
| KspAI | GTTAAC | 1 cut(s) | 1489 |
| Kzo9I | GATC | 7 cut(s) | 418, 496, 583, 688, 827, 1686, 1912 |
| LmnI | GCTCC | 5 cut(s) | 262, 505, 1389, 1400, 2224 |
| Lsp1109I | GCAGC | 4 cut(s) | 176, 876, 948, 1249 |
| LweI | GCATC | 5 cut(s) | 40, 129, 728, 1292, 1666 |
| MabI | ACCWGGT | 1 cut(s) | 1708 |
| MaeI | CTAG | 5 cut(s) | 413, 732, 1943, 2075, 2150 |
| MaeII | ACGT | 1 cut(s) | 766 |
| MaeIII | GTNAC | 5 cut(s) | 56, 386, 452, 1660, 2302 |
| MalI | GATC | 7 cut(s) | 420, 498, 585, 690, 829, 1688, 1914 |
| MbiI | CCGCTC | 1 cut(s) | 1204 |
| MboI | GATC | 7 cut(s) | 418, 496, 583, 688, 827, 1686, 1912 |
| MfeI | CAATTG | 1 cut(s) | 873 |
| MflI | RGATCY | 2 cut(s) | 827, 1912 |
| MlsI | TGGCCA | 4 cut(s) | 1001, 1290, 1892, 2202 |
| MluNI | TGGCCA | 4 cut(s) | 1001, 1290, 1892, 2202 |
| MlyI | GAGTC | 2 cut(s) | 182, 1072 |
| MmeI | TCCRAC | 2 cut(s) | 966, 1329 |
| Mox20I | TGGCCA | 4 cut(s) | 1001, 1290, 1892, 2202 |
| Mph1103I | ATGCAT | 1 cut(s) | 1285 |
| MroI | TCCGGA | 1 cut(s) | 34 |
| MroXI | GAANNNNTTC | 1 cut(s) | 912 |
| MscI | TGGCCA | 4 cut(s) | 1001, 1290, 1892, 2202 |
| MseI | TTAA | 3 cut(s) | 540, 1488, 1983 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1898, 2321 |
| Msp20I | TGGCCA | 4 cut(s) | 1001, 1290, 1892, 2202 |
| MspA1I | CMGCKG | 1 cut(s) | 167 |
| MspI | CCGG | 7 cut(s) | 35, 1692, 1721, 1731, 1736, 1803, 1812 |
| MspR9I | CCNGG | 2 cut(s) | 1710, 1799 |
| MunI | CAATTG | 1 cut(s) | 873 |
| Mva1269I | GAATGC | 2 cut(s) | 1853, 2189 |
| MvaI | CCWGG | 2 cut(s) | 1710, 1799 |
| MvnI | CGCG | 3 cut(s) | 492, 1962, 2250 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 71, 895, 919, 998, 1214, 1226, 1581, 1808, 2135 |
| NdeII | GATC | 7 cut(s) | 418, 496, 583, 688, 827, 1686, 1912 |
| NheI | GCTAGC | 1 cut(s) | 412 |
| NlaIV | GGNNCC | 4 cut(s) | 1714, 1914, 2139, 2226 |
| NmuCI | GTSAC | 1 cut(s) | 386 |
| NruI | TCGCGA | 1 cut(s) | 2250 |
| NsbI | TGCGCA | 1 cut(s) | 5 |
| NsiI | ATGCAT | 1 cut(s) | 1285 |
| NspI | RCATGY | 4 cut(s) | 217, 905, 1283, 1880 |
| PaeI | GCATGC | 1 cut(s) | 217 |
| PaeR7I | CTCGAG | 1 cut(s) | 395 |
| PagI | TCATGA | 1 cut(s) | 916 |
| PciI | ACATGT | 1 cut(s) | 1876 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 636 |
| PctI | GAATGC | 2 cut(s) | 1853, 2189 |
| PdmI | GAANNNNTTC | 1 cut(s) | 912 |
| PfeI | GAWTC | 5 cut(s) | 362, 529, 559, 869, 1775 |
| PflFI | GACNNNGTC | 1 cut(s) | 751 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1088 |
| PkrI | GCNGC | 8 cut(s) | 166, 718, 891, 935, 938, 1219, 1264, 1978 |
| PleI | GAGTC | 2 cut(s) | 181, 1072 |
| PpsI | GAGTC | 2 cut(s) | 181, 1072 |
| PscI | ACATGT | 1 cut(s) | 1876 |
| PshAI | GACNNNNGTC | 2 cut(s) | 516, 1825 |
| Psp1406I | AACGTT | 1 cut(s) | 766 |
| Psp6I | CCWGG | 2 cut(s) | 1708, 1797 |
| PspEI | GGTNACC | 1 cut(s) | 452 |
| PspFI | CCCAGC | 1 cut(s) | 1920 |
| PspGI | CCWGG | 2 cut(s) | 1708, 1797 |
| PspN4I | GGNNCC | 4 cut(s) | 1714, 1914, 2139, 2226 |
| PspPI | GGNCC | 2 cut(s) | 1733, 1820 |
| PsuI | RGATCY | 2 cut(s) | 827, 1912 |
| PsyI | GACNNNGTC | 1 cut(s) | 751 |
| PvuII | CAGCTG | 1 cut(s) | 167 |
| RruI | TCGCGA | 1 cut(s) | 2250 |
| RsaI | GTAC | 3 cut(s) | 290, 1032, 2327 |
| RsaNI | GTAC | 3 cut(s) | 289, 1031, 2326 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1898, 2321 |
| Rsr2I | CGGWCCG | 1 cut(s) | 1733 |
| RsrII | CGGWCCG | 1 cut(s) | 1733 |
| SaqAI | TTAA | 3 cut(s) | 540, 1488, 1983 |
| SatI | GCNGC | 8 cut(s) | 165, 717, 890, 934, 937, 1218, 1263, 1977 |
| Sau3AI | GATC | 7 cut(s) | 418, 496, 583, 688, 827, 1686, 1912 |
| Sau96I | GGNCC | 2 cut(s) | 1733, 1820 |
| ScaI | AGTACT | 1 cut(s) | 1032 |
| SchI | GAGTC | 2 cut(s) | 182, 1072 |
| ScrFI | CCNGG | 2 cut(s) | 1710, 1799 |
| SexAI | ACCWGGT | 1 cut(s) | 1708 |
| SfaNI | GCATC | 5 cut(s) | 40, 129, 728, 1292, 1666 |
| SfcI | CTRYAG | 2 cut(s) | 684, 2043 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 1808 |
| Sfr274I | CTCGAG | 1 cut(s) | 395 |
| SinI | GGWCC | 2 cut(s) | 1733, 1820 |
| SlaI | CTCGAG | 1 cut(s) | 395 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1898, 2321 |
| SmlI | CTYRAG | 3 cut(s) | 395, 1303, 1326 |
| SmoI | CTYRAG | 3 cut(s) | 395, 1303, 1326 |
| SphI | GCATGC | 1 cut(s) | 217 |
| SspI | AATATT | 2 cut(s) | 1117, 1614 |
| SspMI | CTAG | 5 cut(s) | 413, 732, 1943, 2075, 2150 |
| StyD4I | CCNGG | 2 cut(s) | 1708, 1797 |
| StyI | CCWWGG | 1 cut(s) | 1291 |
| TaaI | ACNGT | 4 cut(s) | 635, 1493, 1897, 2032 |
| TaiI | ACGT | 1 cut(s) | 769 |
| TaqI | TCGA | 4 cut(s) | 396, 740, 755, 1773 |
| TaqII | GACCGA | 1 cut(s) | 1731 |
| TatI | WGTACW | 2 cut(s) | 288, 1030 |
| TauI | GCSGC | 4 cut(s) | 719, 936, 1220, 1979 |
| TfiI | GAWTC | 5 cut(s) | 362, 529, 559, 869, 1775 |
| Tru1I | TTAA | 3 cut(s) | 540, 1488, 1983 |
| Tru9I | TTAA | 3 cut(s) | 540, 1488, 1983 |
| TscAI | CASTG | 5 cut(s) | 232, 1572, 1708, 1900, 2037 |
| TseFI | GTSAC | 1 cut(s) | 386 |
| TseI | GCWGC | 4 cut(s) | 164, 889, 936, 1262 |
| Tsp45I | GTSAC | 1 cut(s) | 386 |
| TspDTI | ATGAA | 9 cut(s) | 246, 315, 447, 905, 1758, 1919, 2034, 2145, 2309 |
| TspGWI | ACGGA | 1 cut(s) | 1815 |
| TspRI | CASTG | 5 cut(s) | 232, 1572, 1708, 1900, 2037 |
| Tth111I | GACNNNGTC | 1 cut(s) | 751 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1088 |
| VpaK11BI | GGWCC | 2 cut(s) | 1733, 1820 |
| XapI | RAATTY | 4 cut(s) | 191, 233, 1383, 2117 |
| XceI | RCATGY | 4 cut(s) | 217, 905, 1283, 1880 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1928 |
| XhoI | CTCGAG | 1 cut(s) | 395 |
| XmnI | GAANNNNTTC | 1 cut(s) | 912 |
| XspI | CTAG | 5 cut(s) | 413, 732, 1943, 2075, 2150 |
| ZrmI | AGTACT | 1 cut(s) | 1032 |
| Zsp2I | ATGCAT | 1 cut(s) | 1285 |