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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 2121 |
| Acc65I | GGTACC | 2 cut(s) | 844, 1052 |
| AccB1I | GGYRCC | 2 cut(s) | 844, 1052 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1269 |
| AccBSI | CCGCTC | 2 cut(s) | 195, 610 |
| AccII | CGCG | 2 cut(s) | 2087, 2531 |
| AciI | CCGC | 9 cut(s) | 107, 195, 610, 1213, 1346, 2085, 2174, 2412, 2576 |
| AclI | AACGTT | 1 cut(s) | 1095 |
| AclWI | GGATC | 3 cut(s) | 146, 470, 1972 |
| AcoI | YGGCCR | 3 cut(s) | 118, 633, 830 |
| AcsI | RAATTY | 6 cut(s) | 974, 1284, 1376, 1925, 1948, 2244 |
| AcuI | CTGAAG | 3 cut(s) | 1662, 2112, 2127 |
| AdeI | CACNNNGTG | 1 cut(s) | 1255 |
| AflIII | ACRYGT | 4 cut(s) | 68, 177, 618, 2529 |
| AgeI | ACCGGT | 1 cut(s) | 2006 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 2034, 2066 |
| AluBI | AGCT | 8 cut(s) | 251, 541, 641, 970, 1321, 1610, 2105, 2281 |
| AluI | AGCT | 8 cut(s) | 251, 541, 641, 970, 1321, 1610, 2105, 2281 |
| Alw21I | GWGCWC | 1 cut(s) | 2556 |
| Alw26I | GTCTC | 7 cut(s) | 125, 368, 941, 946, 1778, 2049, 2224 |
| Alw44I | GTGCAC | 1 cut(s) | 2552 |
| AlwI | GGATC | 3 cut(s) | 146, 470, 1972 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 37 |
| Ama87I | CYCGRG | 2 cut(s) | 770, 2375 |
| AoxI | GGCC | 5 cut(s) | 118, 633, 830, 914, 2592 |
| ApaI | GGGCCC | 1 cut(s) | 918 |
| ApaLI | GTGCAC | 1 cut(s) | 2552 |
| ApeKI | GCWGC | 5 cut(s) | 31, 235, 430, 1146, 1318 |
| ApoI | RAATTY | 6 cut(s) | 974, 1284, 1376, 1925, 1948, 2244 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1214, 1246 |
| AsiGI | ACCGGT | 1 cut(s) | 2006 |
| Asp718I | GGTACC | 2 cut(s) | 844, 1052 |
| AspLEI | GCGC | 3 cut(s) | 206, 2089, 2122 |
| AspS9I | GGNCC | 7 cut(s) | 104, 754, 914, 915, 1239, 1526, 2009 |
| AsuC2I | CCSGG | 9 cut(s) | 230, 318, 912, 918, 927, 1702, 1718, 1889, 2573 |
| AsuHPI | GGTGA | 7 cut(s) | 98, 847, 1172, 1961, 2125, 2381, 2537 |
| AvaI | CYCGRG | 2 cut(s) | 770, 2375 |
| AvaII | GGWCC | 5 cut(s) | 104, 754, 1239, 1526, 2009 |
| AxyI | CCTNAGG | 2 cut(s) | 38, 1530 |
| BaeGI | GKGCMC | 3 cut(s) | 918, 1493, 2556 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 566, 599 |
| BalI | TGGCCA | 1 cut(s) | 120 |
| BanI | GGYRCC | 2 cut(s) | 844, 1052 |
| BanII | GRGCYC | 2 cut(s) | 777, 918 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 561, 593, 869, 901 |
| BauI | CACGAG | 2 cut(s) | 1019, 2123 |
| BbsI | GAAGAC | 7 cut(s) | 335, 440, 615, 806, 1520, 1573, 2604 |
| Bbv12I | GWGCWC | 1 cut(s) | 2556 |
| BbvI | GCAGC | 5 cut(s) | 43, 247, 417, 1158, 1330 |
| BccI | CCATC | 7 cut(s) | 343, 642, 1064, 1229, 1263, 1989, 2352 |
| BceAI | ACGGC | 3 cut(s) | 177, 648, 1807 |
| BciVI | GTATCC | 3 cut(s) | 1100, 1683, 2043 |
| BclI | TGATCA | 2 cut(s) | 112, 1431 |
| BcnI | CCSGG | 9 cut(s) | 230, 318, 912, 918, 927, 1702, 1718, 1889, 2573 |
| BcoDI | GTCTC | 7 cut(s) | 125, 368, 941, 946, 1778, 2049, 2224 |
| BfaI | CTAG | 3 cut(s) | 246, 1445, 2282 |
| BfmI | CTRYAG | 2 cut(s) | 410, 549 |
| BfuI | GTATCC | 3 cut(s) | 1100, 1683, 2043 |
| BisI | GCNGC | 8 cut(s) | 32, 236, 431, 1147, 1319, 1347, 2413, 2577 |
| BlpI | GCTNAGC | 1 cut(s) | 1322 |
| BlsI | GCNGC | 8 cut(s) | 33, 237, 432, 1148, 1320, 1348, 2414, 2578 |
| Bme1390I | CCNGG | 9 cut(s) | 230, 318, 912, 918, 927, 1702, 1718, 1889, 2573 |
| Bme18I | GGWCC | 5 cut(s) | 104, 754, 1239, 1526, 2009 |
| BmeT110I | CYCGRG | 2 cut(s) | 770, 2375 |
| BmgT120I | GGNCC | 7 cut(s) | 104, 754, 914, 915, 1239, 1526, 2009 |
| BmiI | GGNNCC | 7 cut(s) | 105, 846, 915, 916, 1054, 1240, 1821 |
| BmrFI | CCNGG | 9 cut(s) | 230, 318, 912, 918, 927, 1702, 1718, 1889, 2573 |
| BmrI | ACTGGG | 1 cut(s) | 1284 |
| BmsI | GCATC | 4 cut(s) | 13, 417, 1023, 1443 |
| BmuI | ACTGGG | 1 cut(s) | 1284 |
| BoxI | GACNNNNGTC | 2 cut(s) | 510, 990 |
| BpiI | GAAGAC | 7 cut(s) | 335, 440, 615, 806, 1520, 1573, 2604 |
| BpmI | CTGGAG | 2 cut(s) | 1438, 2206 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1130, 2520 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1322 |
| BpuEI | CTTGAG | 2 cut(s) | 192, 1590 |
| BpuMI | CCSGG | 9 cut(s) | 230, 318, 912, 918, 927, 1702, 1718, 1889, 2573 |
| Bsa29I | ATCGAT | 1 cut(s) | 7 |
| BsaBI | GATNNNNATC | 2 cut(s) | 405, 945 |
| BsaI | GGTCTC | 1 cut(s) | 946 |
| BsaJI | CCNNGG | 8 cut(s) | 316, 436, 493, 863, 911, 926, 1075, 2037 |
| BsaWI | WCCGGW | 2 cut(s) | 514, 2006 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 599, 629, 1135, 1165 |
| Bse118I | RCCGGY | 3 cut(s) | 832, 2006, 2185 |
| Bse1I | ACTGG | 7 cut(s) | 1279, 1421, 1739, 2012, 2223, 2300, 2336 |
| Bse21I | CCTNAGG | 2 cut(s) | 38, 1530 |
| Bse3DI | GCAATG | 2 cut(s) | 1153, 2286 |
| Bse8I | GATNNNNATC | 2 cut(s) | 405, 945 |
| BseCI | ATCGAT | 1 cut(s) | 7 |
| BseDI | CCNNGG | 8 cut(s) | 316, 436, 493, 863, 911, 926, 1075, 2037 |
| BseJI | GATNNNNATC | 2 cut(s) | 405, 945 |
| BseMI | GCAATG | 2 cut(s) | 1153, 2286 |
| BseMII | CTCAG | 4 cut(s) | 29, 120, 941, 1336 |
| BseNI | ACTGG | 7 cut(s) | 1279, 1421, 1739, 2012, 2223, 2300, 2336 |
| BseRI | GAGGAG | 1 cut(s) | 55 |
| BseSI | GKGCMC | 3 cut(s) | 918, 1493, 2556 |
| BseX3I | CGGCCG | 1 cut(s) | 633 |
| BseXI | GCAGC | 5 cut(s) | 43, 247, 417, 1158, 1330 |
| BseYI | CCCAGC | 1 cut(s) | 1989 |
| BsgI | GTGCAG | 1 cut(s) | 254 |
| Bsh1236I | CGCG | 2 cut(s) | 2087, 2531 |
| Bsh1285I | CGRYCG | 3 cut(s) | 387, 636, 1796 |
| BshFI | GGCC | 5 cut(s) | 120, 635, 832, 916, 2594 |
| BshNI | GGYRCC | 2 cut(s) | 844, 1052 |
| BshTI | ACCGGT | 1 cut(s) | 2006 |
| BshVI | ATCGAT | 1 cut(s) | 7 |
| BsiEI | CGRYCG | 3 cut(s) | 387, 636, 1796 |
| BsiHKAI | GWGCWC | 1 cut(s) | 2556 |
| BsiHKCI | CYCGRG | 2 cut(s) | 770, 2375 |
| BslFI | GGGAC | 3 cut(s) | 998, 1252, 1733 |
| BsmAI | GTCTC | 7 cut(s) | 125, 368, 941, 946, 1778, 2049, 2224 |
| BsmBI | CGTCTC | 2 cut(s) | 125, 1778 |
| BsmFI | GGGAC | 3 cut(s) | 998, 1252, 1733 |
| BsmI | GAATGC | 1 cut(s) | 164 |
| BsnI | GGCC | 5 cut(s) | 120, 635, 832, 916, 2594 |
| Bso31I | GGTCTC | 1 cut(s) | 946 |
| BsoBI | CYCGRG | 2 cut(s) | 770, 2375 |
| Bsp120I | GGGCCC | 1 cut(s) | 914 |
| Bsp1286I | GDGCHC | 4 cut(s) | 777, 918, 1493, 2556 |
| Bsp1407I | TGTACA | 1 cut(s) | 615 |
| Bsp143I | GATC | 7 cut(s) | 112, 151, 475, 1431, 1543, 1977, 2371 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1322 |
| Bsp19I | CCATGG | 2 cut(s) | 863, 2037 |
| BspACI | CCGC | 9 cut(s) | 107, 195, 610, 1213, 1346, 2085, 2174, 2412, 2576 |
| BspANI | GGCC | 5 cut(s) | 120, 635, 832, 916, 2594 |
| BspCNI | CTCAG | 4 cut(s) | 30, 121, 942, 1335 |
| BspDI | ATCGAT | 1 cut(s) | 7 |
| BspFNI | CGCG | 2 cut(s) | 2087, 2531 |
| BspHI | TCATGA | 1 cut(s) | 1747 |
| BspLI | GGNNCC | 7 cut(s) | 105, 846, 915, 916, 1054, 1240, 1821 |
| BspMAI | CTGCAG | 1 cut(s) | 553 |
| BspPI | GGATC | 3 cut(s) | 146, 470, 1972 |
| BspQI | GCTCTTC | 1 cut(s) | 960 |
| BspT107I | GGYRCC | 2 cut(s) | 844, 1052 |
| BspTNI | GGTCTC | 1 cut(s) | 946 |
| BsrBI | CCGCTC | 2 cut(s) | 195, 610 |
| BsrDI | GCAATG | 2 cut(s) | 1153, 2286 |
| BsrFI | RCCGGY | 3 cut(s) | 832, 2006, 2185 |
| BsrGI | TGTACA | 1 cut(s) | 615 |
| BsrI | ACTGG | 7 cut(s) | 1279, 1421, 1739, 2012, 2223, 2300, 2336 |
| BssAI | RCCGGY | 3 cut(s) | 832, 2006, 2185 |
| BssECI | CCNNGG | 8 cut(s) | 316, 436, 493, 863, 911, 926, 1075, 2037 |
| BssMI | GATC | 7 cut(s) | 112, 151, 475, 1431, 1543, 1977, 2371 |
| BssSI | CACGAG | 2 cut(s) | 1019, 2123 |
| BssT1I | CCWWGG | 3 cut(s) | 493, 863, 2037 |
| Bst2BI | CACGAG | 2 cut(s) | 1019, 2123 |
| Bst6I | CTCTTC | 4 cut(s) | 960, 1736, 1781, 2601 |
| BstAPI | GCANNNNNTGC | 4 cut(s) | 1155, 1999, 2117, 2182 |
| BstAUI | TGTACA | 1 cut(s) | 615 |
| BstC8I | GCNNGC | 4 cut(s) | 291, 553, 654, 1537 |
| BstDEI | CTNAG | 7 cut(s) | 38, 129, 950, 1130, 1322, 1530, 2520 |
| BstDSI | CCRYGG | 4 cut(s) | 436, 863, 1075, 2037 |
| BstEII | GGTNACC | 1 cut(s) | 1178 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 2519 |
| BstFNI | CGCG | 2 cut(s) | 2087, 2531 |
| BstHHI | GCGC | 3 cut(s) | 206, 2089, 2122 |
| BstKTI | GATC | 7 cut(s) | 115, 154, 478, 1434, 1546, 1980, 2374 |
| BstMAI | GTCTC | 7 cut(s) | 125, 368, 941, 946, 1778, 2049, 2224 |
| BstMBI | GATC | 7 cut(s) | 112, 151, 475, 1431, 1543, 1977, 2371 |
| BstMCI | CGRYCG | 3 cut(s) | 387, 636, 1796 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 201, 1155, 1999, 2117, 2182 |
| BstNSI | RCATGY | 5 cut(s) | 72, 181, 513, 622, 1040 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 510, 990 |
| BstPI | GGTNACC | 1 cut(s) | 1178 |
| BstSCI | CCNGG | 9 cut(s) | 228, 316, 910, 916, 925, 1700, 1716, 1887, 2571 |
| BstSFI | CTRYAG | 2 cut(s) | 410, 549 |
| BstSLI | GKGCMC | 3 cut(s) | 918, 1493, 2556 |
| BstUI | CGCG | 2 cut(s) | 2087, 2531 |
| BstV1I | GCAGC | 5 cut(s) | 43, 247, 417, 1158, 1330 |
| BstV2I | GAAGAC | 7 cut(s) | 335, 440, 615, 806, 1520, 1573, 2604 |
| BstX2I | RGATCY | 1 cut(s) | 475 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1342 |
| BstYI | RGATCY | 1 cut(s) | 475 |
| BstZI | CGGCCG | 1 cut(s) | 633 |
| Bsu15I | ATCGAT | 1 cut(s) | 7 |
| Bsu36I | CCTNAGG | 2 cut(s) | 38, 1530 |
| BsuI | GTATCC | 3 cut(s) | 1100, 1683, 2043 |
| BsuRI | GGCC | 5 cut(s) | 120, 635, 832, 916, 2594 |
| BsuTUI | ATCGAT | 1 cut(s) | 7 |
| BtgI | CCRYGG | 4 cut(s) | 436, 863, 1075, 2037 |
| BtgZI | GCGATG | 1 cut(s) | 1149 |
| BtsI | GCAGTG | 4 cut(s) | 258, 417, 1299, 2439 |
| Cac8I | GCNNGC | 4 cut(s) | 291, 553, 654, 1537 |
| CaiI | CAGNNNCTG | 1 cut(s) | 37 |
| CciI | TCATGA | 1 cut(s) | 1747 |
| CfoI | GCGC | 3 cut(s) | 206, 2089, 2122 |
| Cfr10I | RCCGGY | 3 cut(s) | 832, 2006, 2185 |
| Cfr13I | GGNCC | 7 cut(s) | 104, 754, 914, 915, 1239, 1526, 2009 |
| ClaI | ATCGAT | 1 cut(s) | 7 |
| CseI | GACGC | 3 cut(s) | 515, 2119, 2537 |
| CspAI | ACCGGT | 1 cut(s) | 2006 |
| DdeI | CTNAG | 7 cut(s) | 38, 129, 950, 1130, 1322, 1530, 2520 |
| DpnI | GATC | 7 cut(s) | 114, 153, 477, 1433, 1545, 1979, 2373 |
| DpnII | GATC | 7 cut(s) | 112, 151, 475, 1431, 1543, 1977, 2371 |
| DraIII | CACNNNGTG | 1 cut(s) | 1255 |
| EaeI | YGGCCR | 3 cut(s) | 118, 633, 830 |
| EagI | CGGCCG | 1 cut(s) | 633 |
| Eam1104I | CTCTTC | 4 cut(s) | 960, 1736, 1781, 2601 |
| EarI | CTCTTC | 4 cut(s) | 960, 1736, 1781, 2601 |
| EclXI | CGGCCG | 1 cut(s) | 633 |
| Eco130I | CCWWGG | 3 cut(s) | 493, 863, 2037 |
| Eco24I | GRGCYC | 2 cut(s) | 777, 918 |
| Eco31I | GGTCTC | 1 cut(s) | 946 |
| Eco47I | GGWCC | 5 cut(s) | 104, 754, 1239, 1526, 2009 |
| Eco52I | CGGCCG | 1 cut(s) | 633 |
| Eco57I | CTGAAG | 3 cut(s) | 1662, 2112, 2127 |
| Eco81I | CCTNAGG | 2 cut(s) | 38, 1530 |
| Eco88I | CYCGRG | 2 cut(s) | 770, 2375 |
| Eco91I | GGTNACC | 1 cut(s) | 1178 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 2519 |
| EcoO109I | RGGNCCY | 1 cut(s) | 914 |
| EcoO65I | GGTNACC | 1 cut(s) | 1178 |
| EcoT14I | CCWWGG | 3 cut(s) | 493, 863, 2037 |
| EcoT22I | ATGCAT | 2 cut(s) | 6, 1038 |
| EcoT38I | GRGCYC | 2 cut(s) | 777, 918 |
| ErhI | CCWWGG | 3 cut(s) | 493, 863, 2037 |
| Esp3I | CGTCTC | 2 cut(s) | 125, 1778 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 669, 701 |
| FaqI | GGGAC | 3 cut(s) | 998, 1252, 1733 |
| FbaI | TGATCA | 2 cut(s) | 112, 1431 |
| Fnu4HI | GCNGC | 8 cut(s) | 32, 236, 431, 1147, 1319, 1347, 2413, 2577 |
| FriOI | GRGCYC | 2 cut(s) | 777, 918 |
| Fsp4HI | GCNGC | 8 cut(s) | 32, 236, 431, 1147, 1319, 1347, 2413, 2577 |
| FspBI | CTAG | 3 cut(s) | 246, 1445, 2282 |
| FspI | TGCGCA | 1 cut(s) | 2121 |
| GlaI | GCGC | 3 cut(s) | 205, 2088, 2121 |
| GluI | GCNGC | 8 cut(s) | 32, 236, 431, 1147, 1319, 1347, 2413, 2577 |
| GsaI | CCCAGC | 1 cut(s) | 1993 |
| GsuI | CTGGAG | 2 cut(s) | 1438, 2206 |
| HaeIII | GGCC | 5 cut(s) | 120, 635, 832, 916, 2594 |
| HgaI | GACGC | 3 cut(s) | 515, 2119, 2537 |
| HhaI | GCGC | 3 cut(s) | 206, 2089, 2122 |
| Hin6I | GCGC | 3 cut(s) | 204, 2087, 2120 |
| HinP1I | GCGC | 3 cut(s) | 204, 2087, 2120 |
| HincII | GTYRAC | 1 cut(s) | 2545 |
| HindII | GTYRAC | 1 cut(s) | 2545 |
| HinfI | GANTC | 5 cut(s) | 544, 811, 907, 1744, 2255 |
| HphI | GGTGA | 7 cut(s) | 98, 847, 1172, 1961, 2125, 2381, 2537 |
| Hpy188I | TCNGA | 6 cut(s) | 130, 327, 751, 758, 784, 951 |
| Hpy99I | CGWCG | 1 cut(s) | 788 |
| HpyCH4IV | ACGT | 5 cut(s) | 354, 987, 1095, 1771, 2361 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 201, 1155, 1999, 2117, 2182 |
| HpyF3I | CTNAG | 7 cut(s) | 38, 129, 950, 1130, 1322, 1530, 2520 |
| HpySE526I | ACGT | 5 cut(s) | 354, 987, 1095, 1771, 2361 |
| HspAI | GCGC | 3 cut(s) | 204, 2087, 2120 |
| KpnI | GGTACC | 2 cut(s) | 848, 1056 |
| Ksp22I | TGATCA | 2 cut(s) | 112, 1431 |
| Kzo9I | GATC | 7 cut(s) | 112, 151, 475, 1431, 1543, 1977, 2371 |
| LguI | GCTCTTC | 1 cut(s) | 960 |
| LmnI | GCTCC | 5 cut(s) | 607, 1143, 1615, 1819, 2156 |
| Lsp1109I | GCAGC | 5 cut(s) | 43, 247, 417, 1158, 1330 |
| LweI | GCATC | 4 cut(s) | 13, 417, 1023, 1443 |
| MaeI | CTAG | 3 cut(s) | 246, 1445, 2282 |
| MaeII | ACGT | 5 cut(s) | 354, 987, 1095, 1771, 2361 |
| MaeIII | GTNAC | 5 cut(s) | 44, 521, 1178, 2131, 2525 |
| MalI | GATC | 7 cut(s) | 114, 153, 477, 1433, 1545, 1979, 2373 |
| MbiI | CCGCTC | 2 cut(s) | 195, 610 |
| MboI | GATC | 7 cut(s) | 112, 151, 475, 1431, 1543, 1977, 2371 |
| MfeI | CAATTG | 1 cut(s) | 1994 |
| MflI | RGATCY | 1 cut(s) | 475 |
| MhlI | GDGCHC | 4 cut(s) | 777, 918, 1493, 2556 |
| MlsI | TGGCCA | 1 cut(s) | 120 |
| MluI | ACGCGT | 1 cut(s) | 2529 |
| MluNI | TGGCCA | 1 cut(s) | 120 |
| MlyI | GAGTC | 4 cut(s) | 538, 820, 1753, 2249 |
| MmeI | TCCRAC | 5 cut(s) | 171, 179, 322, 585, 807 |
| Mox20I | TGGCCA | 1 cut(s) | 120 |
| Mph1103I | ATGCAT | 2 cut(s) | 6, 1038 |
| MscI | TGGCCA | 1 cut(s) | 120 |
| MseI | TTAA | 5 cut(s) | 366, 972, 1299, 1562, 2310 |
| MslI | CAYNNNNRTG | 4 cut(s) | 114, 868, 2027, 2138 |
| Msp20I | TGGCCA | 1 cut(s) | 120 |
| MspR9I | CCNGG | 9 cut(s) | 230, 318, 912, 918, 927, 1702, 1718, 1889, 2573 |
| MunI | CAATTG | 1 cut(s) | 1994 |
| Mva1269I | GAATGC | 1 cut(s) | 164 |
| MvnI | CGCG | 2 cut(s) | 2087, 2531 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 201, 1155, 1999, 2117, 2182 |
| NciI | CCSGG | 9 cut(s) | 230, 318, 912, 918, 927, 1702, 1718, 1889, 2573 |
| NcoI | CCATGG | 2 cut(s) | 863, 2037 |
| NdeII | GATC | 7 cut(s) | 112, 151, 475, 1431, 1543, 1977, 2371 |
| NlaIV | GGNNCC | 7 cut(s) | 105, 846, 915, 916, 1054, 1240, 1821 |
| NmuCI | GTSAC | 4 cut(s) | 521, 1178, 2131, 2525 |
| NsbI | TGCGCA | 1 cut(s) | 2121 |
| NsiI | ATGCAT | 2 cut(s) | 6, 1038 |
| NspI | RCATGY | 5 cut(s) | 72, 181, 513, 622, 1040 |
| PaeR7I | CTCGAG | 1 cut(s) | 2375 |
| PagI | TCATGA | 1 cut(s) | 1747 |
| PciI | ACATGT | 3 cut(s) | 68, 177, 618 |
| PciSI | GCTCTTC | 1 cut(s) | 960 |
| PctI | GAATGC | 1 cut(s) | 164 |
| PfeI | GAWTC | 1 cut(s) | 907 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1269 |
| PfoI | TCCNGGA | 2 cut(s) | 1716, 1887 |
| PinAI | ACCGGT | 1 cut(s) | 2006 |
| PkrI | GCNGC | 8 cut(s) | 33, 237, 432, 1148, 1320, 1348, 2414, 2578 |
| PleI | GAGTC | 4 cut(s) | 538, 819, 1752, 2249 |
| PpsI | GAGTC | 4 cut(s) | 538, 819, 1752, 2249 |
| PscI | ACATGT | 3 cut(s) | 68, 177, 618 |
| PshAI | GACNNNNGTC | 2 cut(s) | 510, 990 |
| Psp1406I | AACGTT | 1 cut(s) | 1095 |
| PspEI | GGTNACC | 1 cut(s) | 1178 |
| PspFI | CCCAGC | 1 cut(s) | 1989 |
| PspN4I | GGNNCC | 7 cut(s) | 105, 846, 915, 916, 1054, 1240, 1821 |
| PspOMI | GGGCCC | 1 cut(s) | 914 |
| PspPI | GGNCC | 7 cut(s) | 104, 754, 914, 915, 1239, 1526, 2009 |
| PstI | CTGCAG | 1 cut(s) | 553 |
| PstNI | CAGNNNCTG | 1 cut(s) | 37 |
| PsuI | RGATCY | 1 cut(s) | 475 |
| RseI | CAYNNNNRTG | 4 cut(s) | 114, 868, 2027, 2138 |
| SapI | GCTCTTC | 1 cut(s) | 960 |
| SaqAI | TTAA | 5 cut(s) | 366, 972, 1299, 1562, 2310 |
| SatI | GCNGC | 8 cut(s) | 32, 236, 431, 1147, 1319, 1347, 2413, 2577 |
| Sau3AI | GATC | 7 cut(s) | 112, 151, 475, 1431, 1543, 1977, 2371 |
| Sau96I | GGNCC | 7 cut(s) | 104, 754, 914, 915, 1239, 1526, 2009 |
| SbfI | CCTGCAGG | 1 cut(s) | 553 |
| SchI | GAGTC | 4 cut(s) | 538, 820, 1753, 2249 |
| ScrFI | CCNGG | 9 cut(s) | 230, 318, 912, 918, 927, 1702, 1718, 1889, 2573 |
| SdaI | CCTGCAGG | 1 cut(s) | 553 |
| SduI | GDGCHC | 4 cut(s) | 777, 918, 1493, 2556 |
| SfaNI | GCATC | 4 cut(s) | 13, 417, 1023, 1443 |
| SfcI | CTRYAG | 2 cut(s) | 410, 549 |
| Sfr274I | CTCGAG | 1 cut(s) | 2375 |
| SinI | GGWCC | 5 cut(s) | 104, 754, 1239, 1526, 2009 |
| SlaI | CTCGAG | 1 cut(s) | 2375 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 114, 868, 2027, 2138 |
| SmlI | CTYRAG | 3 cut(s) | 207, 1605, 2375 |
| SmoI | CTYRAG | 3 cut(s) | 207, 1605, 2375 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 553 |
| SsiI | CCGC | 9 cut(s) | 107, 195, 610, 1213, 1346, 2085, 2174, 2412, 2576 |
| SspI | AATATT | 1 cut(s) | 2427 |
| SspMI | CTAG | 3 cut(s) | 246, 1445, 2282 |
| StyD4I | CCNGG | 9 cut(s) | 228, 316, 910, 916, 925, 1700, 1716, 1887, 2571 |
| StyI | CCWWGG | 3 cut(s) | 493, 863, 2037 |
| TaiI | ACGT | 5 cut(s) | 357, 990, 1098, 1774, 2364 |
| TaqI | TCGA | 8 cut(s) | 7, 383, 425, 939, 1218, 1792, 2376, 2560 |
| TatI | WGTACW | 8 cut(s) | 63, 140, 276, 414, 615, 1011, 1731, 2014 |
| TauI | GCSGC | 3 cut(s) | 1349, 2415, 2579 |
| TfiI | GAWTC | 1 cut(s) | 907 |
| Tru1I | TTAA | 5 cut(s) | 366, 972, 1299, 1562, 2310 |
| Tru9I | TTAA | 5 cut(s) | 366, 972, 1299, 1562, 2310 |
| TseFI | GTSAC | 4 cut(s) | 521, 1178, 2131, 2525 |
| TseI | GCWGC | 5 cut(s) | 31, 235, 430, 1146, 1318 |
| Tsp45I | GTSAC | 4 cut(s) | 521, 1178, 2131, 2525 |
| TspDTI | ATGAA | 5 cut(s) | 157, 374, 890, 1777, 2156 |
| TspGWI | ACGGA | 1 cut(s) | 2436 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1269 |
| VneI | GTGCAC | 1 cut(s) | 2552 |
| VpaK11BI | GGWCC | 5 cut(s) | 104, 754, 1239, 1526, 2009 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 2519 |
| XapI | RAATTY | 6 cut(s) | 974, 1284, 1376, 1925, 1948, 2244 |
| XceI | RCATGY | 5 cut(s) | 72, 181, 513, 622, 1040 |
| XhoI | CTCGAG | 1 cut(s) | 2375 |
| XspI | CTAG | 3 cut(s) | 246, 1445, 2282 |
| Zsp2I | ATGCAT | 2 cut(s) | 6, 1038 |