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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 1352 |
| AccI | GTMKAC | 1 cut(s) | 278 |
| AccII | CGCG | 3 cut(s) | 28, 338, 1393 |
| AccIII | TCCGGA | 1 cut(s) | 2071 |
| AciI | CCGC | 8 cut(s) | 26, 131, 217, 336, 1021, 1522, 1658, 1769 |
| AclWI | GGATC | 4 cut(s) | 182, 564, 1252, 1706 |
| AcoI | YGGCCR | 2 cut(s) | 101, 218 |
| AcsI | RAATTY | 3 cut(s) | 575, 875, 1612 |
| AcuI | CTGAAG | 1 cut(s) | 204 |
| AcvI | CACGTG | 1 cut(s) | 1120 |
| AcyI | GRCGYC | 1 cut(s) | 478 |
| AfaI | GTAC | 5 cut(s) | 5, 265, 739, 1818, 1949 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 688, 928, 1228, 1415, 1762, 1768, 1769, 2015 |
| AgeI | ACCGGT | 1 cut(s) | 928 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1732 |
| AhlI | ACTAGT | 1 cut(s) | 1808 |
| AjiI | CACGTC | 1 cut(s) | 1759 |
| AjnI | CCWGG | 1 cut(s) | 190 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1182, 1214 |
| AluBI | AGCT | 4 cut(s) | 20, 677, 1097, 1319 |
| AluI | AGCT | 4 cut(s) | 20, 677, 1097, 1319 |
| Alw26I | GTCTC | 3 cut(s) | 506, 1307, 1902 |
| AlwI | GGATC | 4 cut(s) | 182, 564, 1252, 1706 |
| Ama87I | CYCGRG | 1 cut(s) | 1762 |
| Aor13HI | TCCGGA | 1 cut(s) | 2071 |
| AoxI | GGCC | 4 cut(s) | 101, 218, 707, 751 |
| ApeKI | GCWGC | 2 cut(s) | 17, 305 |
| ApoI | RAATTY | 3 cut(s) | 575, 875, 1612 |
| AseI | ATTAAT | 1 cut(s) | 176 |
| AsiGI | ACCGGT | 1 cut(s) | 928 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1566 |
| AspLEI | GCGC | 2 cut(s) | 1393, 1453 |
| AspS9I | GGNCC | 2 cut(s) | 1157, 1655 |
| AsuC2I | CCSGG | 2 cut(s) | 1763, 1764 |
| AsuHPI | GGTGA | 5 cut(s) | 286, 976, 1214, 1608, 2153 |
| AsuII | TTCGAA | 1 cut(s) | 1077 |
| AvaI | CYCGRG | 1 cut(s) | 1762 |
| AvaII | GGWCC | 2 cut(s) | 1157, 1655 |
| BbrPI | CACGTG | 1 cut(s) | 1120 |
| BbsI | GAAGAC | 2 cut(s) | 1788, 1962 |
| BbvI | GCAGC | 2 cut(s) | 29, 317 |
| BccI | CCATC | 6 cut(s) | 224, 447, 718, 762, 821, 1167 |
| BceAI | ACGGC | 2 cut(s) | 205, 517 |
| BciT130I | CCWGG | 1 cut(s) | 192 |
| BciVI | GTATCC | 1 cut(s) | 1191 |
| BcnI | CCSGG | 2 cut(s) | 1763, 1764 |
| BcoDI | GTCTC | 3 cut(s) | 506, 1307, 1902 |
| BcuI | ACTAGT | 1 cut(s) | 1808 |
| BfaI | CTAG | 4 cut(s) | 987, 1809, 1935, 2000 |
| BfuI | GTATCC | 1 cut(s) | 1191 |
| BisI | GCNGC | 4 cut(s) | 18, 131, 218, 306 |
| BlsI | GCNGC | 4 cut(s) | 19, 132, 219, 307 |
| Bme1390I | CCNGG | 3 cut(s) | 192, 1763, 1764 |
| Bme18I | GGWCC | 2 cut(s) | 1157, 1655 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1732 |
| BmeT110I | CYCGRG | 1 cut(s) | 1762 |
| BmgBI | CACGTC | 1 cut(s) | 1759 |
| BmgT120I | GGNCC | 2 cut(s) | 1157, 1655 |
| BmiI | GGNNCC | 1 cut(s) | 458 |
| BmrFI | CCNGG | 3 cut(s) | 192, 1763, 1764 |
| BmrI | ACTGGG | 1 cut(s) | 291 |
| BmuI | ACTGGG | 1 cut(s) | 291 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1839 |
| BpiI | GAAGAC | 2 cut(s) | 1788, 1962 |
| BpmI | CTGGAG | 2 cut(s) | 561, 1077 |
| Bpu14I | TTCGAA | 1 cut(s) | 1077 |
| BpuEI | CTTGAG | 1 cut(s) | 1663 |
| BpuMI | CCSGG | 2 cut(s) | 1763, 1764 |
| BsaAI | YACGTR | 1 cut(s) | 1120 |
| BsaHI | GRCGYC | 1 cut(s) | 478 |
| BsaI | GGTCTC | 1 cut(s) | 1902 |
| BsaJI | CCNNGG | 5 cut(s) | 134, 461, 499, 1762, 1912 |
| BsaWI | WCCGGW | 2 cut(s) | 928, 2071 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 688, 928, 1228, 1415, 1762, 1768, 1769, 2015 |
| Bse118I | RCCGGY | 3 cut(s) | 103, 928, 2015 |
| Bse1I | ACTGG | 3 cut(s) | 286, 635, 1896 |
| Bse3DI | GCAATG | 4 cut(s) | 351, 1145, 1299, 2026 |
| BseAI | TCCGGA | 1 cut(s) | 2071 |
| BseBI | CCWGG | 1 cut(s) | 192 |
| BseDI | CCNNGG | 5 cut(s) | 134, 461, 499, 1762, 1912 |
| BseGI | GGATG | 6 cut(s) | 366, 710, 726, 754, 1036, 1182 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 688, 928, 1228, 1415, 1762, 1768, 1769, 2015 |
| BseMI | GCAATG | 4 cut(s) | 351, 1145, 1299, 2026 |
| BseNI | ACTGG | 3 cut(s) | 286, 635, 1896 |
| BseX3I | CGGCCG | 1 cut(s) | 218 |
| BseXI | GCAGC | 2 cut(s) | 29, 317 |
| Bsh1236I | CGCG | 3 cut(s) | 28, 338, 1393 |
| Bsh1285I | CGRYCG | 3 cut(s) | 108, 221, 672 |
| BshFI | GGCC | 4 cut(s) | 103, 220, 709, 753 |
| BshTI | ACCGGT | 1 cut(s) | 928 |
| BsiEI | CGRYCG | 3 cut(s) | 108, 221, 672 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1762 |
| BsiSI | CCGG | 5 cut(s) | 104, 929, 1763, 2016, 2072 |
| BslFI | GGGAC | 2 cut(s) | 57, 1745 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 688, 928, 1228, 1415, 1762, 1768, 1769, 2015 |
| BsmAI | GTCTC | 3 cut(s) | 506, 1307, 1902 |
| BsmFI | GGGAC | 2 cut(s) | 57, 1745 |
| BsnI | GGCC | 4 cut(s) | 103, 220, 709, 753 |
| Bso31I | GGTCTC | 1 cut(s) | 1902 |
| BsoBI | CYCGRG | 1 cut(s) | 1762 |
| Bsp119I | TTCGAA | 1 cut(s) | 1077 |
| Bsp13I | TCCGGA | 1 cut(s) | 2071 |
| Bsp143I | GATC | 5 cut(s) | 187, 556, 1244, 1698, 2047 |
| Bsp19I | CCATGG | 1 cut(s) | 1912 |
| BspACI | CCGC | 8 cut(s) | 26, 131, 217, 336, 1021, 1522, 1658, 1769 |
| BspANI | GGCC | 4 cut(s) | 103, 220, 709, 753 |
| BspEI | TCCGGA | 1 cut(s) | 2071 |
| BspFNI | CGCG | 3 cut(s) | 28, 338, 1393 |
| BspHI | TCATGA | 1 cut(s) | 895 |
| BspLI | GGNNCC | 1 cut(s) | 458 |
| BspPI | GGATC | 4 cut(s) | 182, 564, 1252, 1706 |
| BspQI | GCTCTTC | 1 cut(s) | 1494 |
| BspT104I | TTCGAA | 1 cut(s) | 1077 |
| BspTNI | GGTCTC | 1 cut(s) | 1902 |
| BsrDI | GCAATG | 4 cut(s) | 351, 1145, 1299, 2026 |
| BsrFI | RCCGGY | 3 cut(s) | 103, 928, 2015 |
| BsrI | ACTGG | 3 cut(s) | 286, 635, 1896 |
| BssAI | RCCGGY | 3 cut(s) | 103, 928, 2015 |
| BssECI | CCNNGG | 5 cut(s) | 134, 461, 499, 1762, 1912 |
| BssMI | GATC | 5 cut(s) | 187, 556, 1244, 1698, 2047 |
| BssNAI | GTATAC | 1 cut(s) | 279 |
| BssNI | GRCGYC | 1 cut(s) | 478 |
| BssT1I | CCWWGG | 3 cut(s) | 134, 461, 1912 |
| Bst1107I | GTATAC | 1 cut(s) | 279 |
| Bst2UI | CCWGG | 1 cut(s) | 192 |
| Bst6I | CTCTTC | 1 cut(s) | 1494 |
| BstACI | GRCGYC | 1 cut(s) | 478 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1151 |
| BstBAI | YACGTR | 1 cut(s) | 1120 |
| BstBI | TTCGAA | 1 cut(s) | 1077 |
| BstC8I | GCNNGC | 6 cut(s) | 420, 679, 1152, 1406, 1771, 2017 |
| BstDEI | CTNAG | 1 cut(s) | 366 |
| BstDSI | CCRYGG | 2 cut(s) | 499, 1912 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1413 |
| BstF5I | GGATG | 6 cut(s) | 366, 710, 726, 754, 1036, 1182 |
| BstFNI | CGCG | 3 cut(s) | 28, 338, 1393 |
| BstHHI | GCGC | 2 cut(s) | 1393, 1453 |
| BstKTI | GATC | 5 cut(s) | 190, 559, 1247, 1701, 2050 |
| BstMAI | GTCTC | 3 cut(s) | 506, 1307, 1902 |
| BstMBI | GATC | 5 cut(s) | 187, 556, 1244, 1698, 2047 |
| BstMCI | CGRYCG | 3 cut(s) | 108, 221, 672 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 1151, 1388, 1979 |
| BstNI | CCWGG | 1 cut(s) | 192 |
| BstNSI | RCATGY | 2 cut(s) | 384, 422 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1839 |
| BstSCI | CCNGG | 3 cut(s) | 190, 1761, 1762 |
| BstUI | CGCG | 3 cut(s) | 28, 338, 1393 |
| BstV1I | GCAGC | 2 cut(s) | 29, 317 |
| BstV2I | GAAGAC | 2 cut(s) | 1788, 1962 |
| BstX2I | RGATCY | 1 cut(s) | 187 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 534, 717 |
| BstYI | RGATCY | 1 cut(s) | 187 |
| BstZ17I | GTATAC | 1 cut(s) | 279 |
| BstZI | CGGCCG | 1 cut(s) | 218 |
| BsuI | GTATCC | 1 cut(s) | 1191 |
| BsuRI | GGCC | 4 cut(s) | 103, 220, 709, 753 |
| BtgI | CCRYGG | 2 cut(s) | 499, 1912 |
| BtgZI | GCGATG | 1 cut(s) | 2089 |
| BtrI | CACGTC | 1 cut(s) | 1759 |
| BtsCI | GGATG | 6 cut(s) | 366, 710, 726, 754, 1036, 1182 |
| BtsI | GCAGTG | 1 cut(s) | 315 |
| BtsIMutI | CAGTG | 3 cut(s) | 315, 1783, 1903 |
| Cac8I | GCNNGC | 6 cut(s) | 420, 679, 1152, 1406, 1771, 2017 |
| CciI | TCATGA | 1 cut(s) | 895 |
| CfoI | GCGC | 2 cut(s) | 1393, 1453 |
| Cfr10I | RCCGGY | 3 cut(s) | 103, 928, 2015 |
| Cfr13I | GGNCC | 2 cut(s) | 1157, 1655 |
| Cfr9I | CCCGGG | 1 cut(s) | 1762 |
| CseI | GACGC | 1 cut(s) | 467 |
| Csp6I | GTAC | 5 cut(s) | 4, 264, 738, 1817, 1948 |
| CspAI | ACCGGT | 1 cut(s) | 928 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 80, 115 |
| CviQI | GTAC | 5 cut(s) | 4, 264, 738, 1817, 1948 |
| DdeI | CTNAG | 1 cut(s) | 366 |
| DpnI | GATC | 5 cut(s) | 189, 558, 1246, 1700, 2049 |
| DpnII | GATC | 5 cut(s) | 187, 556, 1244, 1698, 2047 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1352 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1732 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1352 |
| EaeI | YGGCCR | 2 cut(s) | 101, 218 |
| EagI | CGGCCG | 1 cut(s) | 218 |
| Eam1104I | CTCTTC | 1 cut(s) | 1494 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1732 |
| EarI | CTCTTC | 1 cut(s) | 1494 |
| EclXI | CGGCCG | 1 cut(s) | 218 |
| Eco130I | CCWWGG | 3 cut(s) | 134, 461, 1912 |
| Eco31I | GGTCTC | 1 cut(s) | 1902 |
| Eco32I | GATATC | 1 cut(s) | 1196 |
| Eco47I | GGWCC | 2 cut(s) | 1157, 1655 |
| Eco52I | CGGCCG | 1 cut(s) | 218 |
| Eco57I | CTGAAG | 1 cut(s) | 204 |
| Eco72I | CACGTG | 1 cut(s) | 1120 |
| Eco88I | CYCGRG | 1 cut(s) | 1762 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1413 |
| EcoRI | GAATTC | 1 cut(s) | 1612 |
| EcoRII | CCWGG | 1 cut(s) | 190 |
| EcoRV | GATATC | 1 cut(s) | 1196 |
| EcoT14I | CCWWGG | 3 cut(s) | 134, 461, 1912 |
| ErhI | CCWWGG | 3 cut(s) | 134, 461, 1912 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 666, 698, 1233, 1265 |
| FaqI | GGGAC | 2 cut(s) | 57, 1745 |
| FauI | CCCGC | 4 cut(s) | 33, 343, 1515, 1762 |
| FauNDI | CATATG | 3 cut(s) | 268, 1270, 1590 |
| FblI | GTMKAC | 1 cut(s) | 278 |
| Fnu4HI | GCNGC | 4 cut(s) | 18, 131, 218, 306 |
| FokI | GGATG | 6 cut(s) | 373, 697, 733, 741, 1043, 1189 |
| Fsp4HI | GCNGC | 4 cut(s) | 18, 131, 218, 306 |
| FspBI | CTAG | 4 cut(s) | 987, 1809, 1935, 2000 |
| GlaI | GCGC | 2 cut(s) | 1392, 1452 |
| GluI | GCNGC | 4 cut(s) | 18, 131, 218, 306 |
| GsuI | CTGGAG | 2 cut(s) | 561, 1077 |
| HaeIII | GGCC | 4 cut(s) | 103, 220, 709, 753 |
| HapII | CCGG | 5 cut(s) | 104, 929, 1763, 2016, 2072 |
| HgaI | GACGC | 1 cut(s) | 467 |
| HhaI | GCGC | 2 cut(s) | 1393, 1453 |
| Hin1I | GRCGYC | 1 cut(s) | 478 |
| Hin6I | GCGC | 2 cut(s) | 1391, 1451 |
| HinP1I | GCGC | 2 cut(s) | 1391, 1451 |
| HincII | GTYRAC | 2 cut(s) | 168, 1693 |
| HindII | GTYRAC | 2 cut(s) | 168, 1693 |
| HinfI | GANTC | 6 cut(s) | 169, 514, 1114, 1126, 1274, 1421 |
| HpaII | CCGG | 5 cut(s) | 104, 929, 1763, 2016, 2072 |
| HphI | GGTGA | 5 cut(s) | 286, 976, 1214, 1608, 2153 |
| Hpy188I | TCNGA | 9 cut(s) | 38, 483, 627, 777, 1030, 1223, 1358, 2040, 2156 |
| Hpy99I | CGWCG | 2 cut(s) | 59, 113 |
| HpyAV | CCTTC | 6 cut(s) | 505, 644, 932, 1235, 1581, 1597 |
| HpyCH4IV | ACGT | 8 cut(s) | 33, 402, 850, 861, 1062, 1119, 1665, 1758 |
| HpyCH4V | TGCA | 8 cut(s) | 17, 144, 344, 393, 1010, 1045, 1071, 1292 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 1151, 1388, 1979 |
| HpyF3I | CTNAG | 1 cut(s) | 366 |
| HpySE526I | ACGT | 8 cut(s) | 33, 402, 850, 861, 1062, 1119, 1665, 1758 |
| Hsp92I | GRCGYC | 1 cut(s) | 478 |
| HspAI | GCGC | 2 cut(s) | 1391, 1451 |
| Kpn2I | TCCGGA | 1 cut(s) | 2071 |
| KroI | GCCGGC | 1 cut(s) | 2015 |
| KroNI | GCCGGC | 1 cut(s) | 2017 |
| Kzo9I | GATC | 5 cut(s) | 187, 556, 1244, 1698, 2047 |
| LguI | GCTCTTC | 1 cut(s) | 1494 |
| LmnI | GCTCC | 1 cut(s) | 462 |
| Lsp1109I | GCAGC | 2 cut(s) | 29, 317 |
| MaeI | CTAG | 4 cut(s) | 987, 1809, 1935, 2000 |
| MaeII | ACGT | 8 cut(s) | 33, 402, 850, 861, 1062, 1119, 1665, 1758 |
| MaeIII | GTNAC | 2 cut(s) | 403, 1275 |
| MalI | GATC | 5 cut(s) | 189, 558, 1246, 1700, 2049 |
| MboI | GATC | 5 cut(s) | 187, 556, 1244, 1698, 2047 |
| MfeI | CAATTG | 5 cut(s) | 1001, 1040, 1165, 1471, 1820 |
| MflI | RGATCY | 1 cut(s) | 187 |
| MlyI | GAGTC | 3 cut(s) | 163, 508, 1283 |
| MmeI | TCCRAC | 3 cut(s) | 940, 1008, 2006 |
| MroI | TCCGGA | 1 cut(s) | 2071 |
| MroNI | GCCGGC | 1 cut(s) | 2015 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1566 |
| MseI | TTAA | 7 cut(s) | 63, 176, 563, 771, 1035, 1857, 1902 |
| MslI | CAYNNNNRTG | 2 cut(s) | 715, 1542 |
| MspA1I | CMGCKG | 1 cut(s) | 20 |
| MspI | CCGG | 5 cut(s) | 104, 929, 1763, 2016, 2072 |
| MspR9I | CCNGG | 3 cut(s) | 192, 1763, 1764 |
| MunI | CAATTG | 5 cut(s) | 1001, 1040, 1165, 1471, 1820 |
| MvaI | CCWGG | 1 cut(s) | 192 |
| MvnI | CGCG | 3 cut(s) | 28, 338, 1393 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 1151, 1388, 1979 |
| NaeI | GCCGGC | 1 cut(s) | 2017 |
| NciI | CCSGG | 2 cut(s) | 1763, 1764 |
| NcoI | CCATGG | 1 cut(s) | 1912 |
| NdeI | CATATG | 3 cut(s) | 268, 1270, 1590 |
| NdeII | GATC | 5 cut(s) | 187, 556, 1244, 1698, 2047 |
| NgoMIV | GCCGGC | 1 cut(s) | 2015 |
| NlaIV | GGNNCC | 1 cut(s) | 458 |
| NmuCI | GTSAC | 1 cut(s) | 1275 |
| NspI | RCATGY | 2 cut(s) | 384, 422 |
| NspV | TTCGAA | 1 cut(s) | 1077 |
| PaeI | GCATGC | 1 cut(s) | 422 |
| PagI | TCATGA | 1 cut(s) | 895 |
| PciSI | GCTCTTC | 1 cut(s) | 1494 |
| PdiI | GCCGGC | 1 cut(s) | 2017 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1566 |
| PfeI | GAWTC | 3 cut(s) | 1114, 1126, 1421 |
| PflFI | GACNNNGTC | 1 cut(s) | 668 |
| PfoI | TCCNGGA | 1 cut(s) | 190 |
| PinAI | ACCGGT | 1 cut(s) | 928 |
| PkrI | GCNGC | 4 cut(s) | 19, 132, 219, 307 |
| PleI | GAGTC | 3 cut(s) | 163, 508, 1282 |
| PmaCI | CACGTG | 1 cut(s) | 1120 |
| PmlI | CACGTG | 1 cut(s) | 1120 |
| PpsI | GAGTC | 3 cut(s) | 163, 508, 1282 |
| Ppu21I | YACGTR | 1 cut(s) | 1120 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1839 |
| PshBI | ATTAAT | 1 cut(s) | 176 |
| Psp6I | CCWGG | 1 cut(s) | 190 |
| PspCI | CACGTG | 1 cut(s) | 1120 |
| PspGI | CCWGG | 1 cut(s) | 190 |
| PspN4I | GGNNCC | 1 cut(s) | 458 |
| PspPI | GGNCC | 2 cut(s) | 1157, 1655 |
| PsuI | RGATCY | 1 cut(s) | 187 |
| PsyI | GACNNNGTC | 1 cut(s) | 668 |
| PvuII | CAGCTG | 1 cut(s) | 20 |
| RsaI | GTAC | 5 cut(s) | 5, 265, 739, 1818, 1949 |
| RsaNI | GTAC | 5 cut(s) | 4, 264, 738, 1817, 1948 |
| RseI | CAYNNNNRTG | 2 cut(s) | 715, 1542 |
| SapI | GCTCTTC | 1 cut(s) | 1494 |
| SaqAI | TTAA | 7 cut(s) | 63, 176, 563, 771, 1035, 1857, 1902 |
| SatI | GCNGC | 4 cut(s) | 18, 131, 218, 306 |
| Sau3AI | GATC | 5 cut(s) | 187, 556, 1244, 1698, 2047 |
| Sau96I | GGNCC | 2 cut(s) | 1157, 1655 |
| SchI | GAGTC | 3 cut(s) | 163, 508, 1283 |
| ScrFI | CCNGG | 3 cut(s) | 192, 1763, 1764 |
| SfuI | TTCGAA | 1 cut(s) | 1077 |
| SinI | GGWCC | 2 cut(s) | 1157, 1655 |
| SmaI | CCCGGG | 1 cut(s) | 1764 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 715, 1542 |
| SmlI | CTYRAG | 1 cut(s) | 1678 |
| SmoI | CTYRAG | 1 cut(s) | 1678 |
| SpeI | ACTAGT | 1 cut(s) | 1808 |
| SphI | GCATGC | 1 cut(s) | 422 |
| SsiI | CCGC | 8 cut(s) | 26, 131, 217, 336, 1021, 1522, 1658, 1769 |
| SspI | AATATT | 2 cut(s) | 1086, 1992 |
| SspMI | CTAG | 4 cut(s) | 987, 1809, 1935, 2000 |
| StyD4I | CCNGG | 3 cut(s) | 190, 1761, 1762 |
| StyI | CCWWGG | 3 cut(s) | 134, 461, 1912 |
| TaiI | ACGT | 8 cut(s) | 36, 405, 853, 864, 1065, 1122, 1668, 1761 |
| TaqI | TCGA | 5 cut(s) | 517, 672, 940, 1077, 2050 |
| TatI | WGTACW | 1 cut(s) | 1816 |
| TauI | GCSGC | 2 cut(s) | 133, 220 |
| TfiI | GAWTC | 3 cut(s) | 1114, 1126, 1421 |
| Tru1I | TTAA | 7 cut(s) | 63, 176, 563, 771, 1035, 1857, 1902 |
| Tru9I | TTAA | 7 cut(s) | 63, 176, 563, 771, 1035, 1857, 1902 |
| TscAI | CASTG | 3 cut(s) | 315, 1783, 1903 |
| TseFI | GTSAC | 1 cut(s) | 1275 |
| TseI | GCWGC | 2 cut(s) | 17, 305 |
| Tsp45I | GTSAC | 1 cut(s) | 1275 |
| TspGWI | ACGGA | 2 cut(s) | 57, 72 |
| TspMI | CCCGGG | 1 cut(s) | 1762 |
| TspRI | CASTG | 3 cut(s) | 315, 1783, 1903 |
| Tth111I | GACNNNGTC | 1 cut(s) | 668 |
| VpaK11BI | GGWCC | 2 cut(s) | 1157, 1655 |
| VspI | ATTAAT | 1 cut(s) | 176 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1413 |
| XapI | RAATTY | 3 cut(s) | 575, 875, 1612 |
| XbaI | TCTAGA | 2 cut(s) | 986, 1999 |
| XceI | RCATGY | 2 cut(s) | 384, 422 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1438 |
| XmaI | CCCGGG | 1 cut(s) | 1762 |
| XmiI | GTMKAC | 1 cut(s) | 278 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1566 |
| XspI | CTAG | 4 cut(s) | 987, 1809, 1935, 2000 |