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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 1638 |
| Acc65I | GGTACC | 2 cut(s) | 703, 911 |
| AccB1I | GGYRCC | 2 cut(s) | 703, 911 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1128 |
| AccBSI | CCGCTC | 1 cut(s) | 469 |
| AccII | CGCG | 1 cut(s) | 1604 |
| AccIII | TCCGGA | 1 cut(s) | 769 |
| AciI | CCGC | 6 cut(s) | 469, 1072, 1205, 1602, 1691, 1930 |
| AclI | AACGTT | 1 cut(s) | 954 |
| AclWI | GGATC | 2 cut(s) | 286, 329 |
| AcoI | YGGCCR | 2 cut(s) | 492, 689 |
| AcsI | RAATTY | 4 cut(s) | 833, 1143, 1235, 1761 |
| AcuI | CTGAAG | 3 cut(s) | 1521, 1629, 1644 |
| AdeI | CACNNNGTG | 1 cut(s) | 1114 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 454, 560, 776, 935, 1128, 1815, 1816, 1906 |
| AflIII | ACRYGT | 1 cut(s) | 477 |
| AluBI | AGCT | 8 cut(s) | 32, 400, 500, 829, 1180, 1469, 1622, 1798 |
| AluI | AGCT | 8 cut(s) | 32, 400, 500, 829, 1180, 1469, 1622, 1798 |
| Alw26I | GTCTC | 5 cut(s) | 149, 800, 805, 1566, 1741 |
| AlwI | GGATC | 2 cut(s) | 286, 329 |
| Ama87I | CYCGRG | 2 cut(s) | 629, 1892 |
| Aor13HI | TCCGGA | 1 cut(s) | 769 |
| AoxI | GGCC | 2 cut(s) | 492, 689 |
| ApeKI | GCWGC | 4 cut(s) | 16, 211, 1005, 1177 |
| ApoI | RAATTY | 4 cut(s) | 833, 1143, 1235, 1761 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1073, 1105 |
| Asp718I | GGTACC | 2 cut(s) | 703, 911 |
| AspLEI | GCGC | 2 cut(s) | 1606, 1639 |
| AspS9I | GGNCC | 4 cut(s) | 250, 613, 1098, 1385 |
| AsuC2I | CCSGG | 5 cut(s) | 11, 99, 777, 786, 1561 |
| AsuHPI | GGTGA | 4 cut(s) | 706, 1031, 1642, 1898 |
| AvaI | CYCGRG | 2 cut(s) | 629, 1892 |
| AvaII | GGWCC | 4 cut(s) | 250, 613, 1098, 1385 |
| AxyI | CCTNAGG | 1 cut(s) | 1389 |
| BaeGI | GKGCMC | 1 cut(s) | 1352 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 425, 458 |
| BanI | GGYRCC | 2 cut(s) | 703, 911 |
| BanII | GRGCYC | 2 cut(s) | 636, 777 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 420, 452, 728, 760 |
| BauI | CACGAG | 2 cut(s) | 878, 1640 |
| BbsI | GAAGAC | 6 cut(s) | 116, 221, 474, 665, 1379, 1432 |
| BbvI | GCAGC | 4 cut(s) | 28, 198, 1017, 1189 |
| BccI | CCATC | 6 cut(s) | 124, 501, 923, 1088, 1122, 1869 |
| BceAI | ACGGC | 1 cut(s) | 507 |
| BciVI | GTATCC | 3 cut(s) | 269, 959, 1542 |
| BclI | TGATCA | 1 cut(s) | 1290 |
| BcnI | CCSGG | 5 cut(s) | 11, 99, 777, 786, 1561 |
| BcoDI | GTCTC | 5 cut(s) | 149, 800, 805, 1566, 1741 |
| BfaI | CTAG | 2 cut(s) | 1304, 1799 |
| BfmI | CTRYAG | 2 cut(s) | 191, 408 |
| BfuI | GTATCC | 3 cut(s) | 269, 959, 1542 |
| BglII | AGATCT | 1 cut(s) | 268 |
| BisI | GCNGC | 6 cut(s) | 17, 212, 1006, 1178, 1206, 1931 |
| BlpI | GCTNAGC | 1 cut(s) | 1181 |
| BlsI | GCNGC | 6 cut(s) | 18, 213, 1007, 1179, 1207, 1932 |
| Bme1390I | CCNGG | 5 cut(s) | 11, 99, 777, 786, 1561 |
| Bme18I | GGWCC | 4 cut(s) | 250, 613, 1098, 1385 |
| BmeT110I | CYCGRG | 2 cut(s) | 629, 1892 |
| BmgT120I | GGNCC | 4 cut(s) | 250, 613, 1098, 1385 |
| BmiI | GGNNCC | 4 cut(s) | 705, 774, 913, 1099 |
| BmrFI | CCNGG | 5 cut(s) | 11, 99, 777, 786, 1561 |
| BmsI | GCATC | 3 cut(s) | 198, 882, 1302 |
| BoxI | GACNNNNGTC | 2 cut(s) | 369, 849 |
| BpiI | GAAGAC | 6 cut(s) | 116, 221, 474, 665, 1379, 1432 |
| BpmI | CTGGAG | 2 cut(s) | 1297, 1723 |
| Bpu10I | CCTNAGC | 1 cut(s) | 989 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1181 |
| BpuEI | CTTGAG | 1 cut(s) | 1449 |
| BpuMI | CCSGG | 5 cut(s) | 11, 99, 777, 786, 1561 |
| BsaAI | YACGTR | 1 cut(s) | 1941 |
| BsaBI | GATNNNNATC | 2 cut(s) | 186, 804 |
| BsaI | GGTCTC | 1 cut(s) | 805 |
| BsaJI | CCNNGG | 6 cut(s) | 97, 217, 352, 722, 785, 934 |
| BsaWI | WCCGGW | 2 cut(s) | 373, 769 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 458, 488, 994, 1024 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 454, 560, 776, 935, 1128, 1815, 1816, 1906 |
| Bse118I | RCCGGY | 2 cut(s) | 691, 1702 |
| Bse1I | ACTGG | 4 cut(s) | 1280, 1740, 1817, 1853 |
| Bse21I | CCTNAGG | 1 cut(s) | 1389 |
| Bse3DI | GCAATG | 2 cut(s) | 1012, 1803 |
| Bse8I | GATNNNNATC | 2 cut(s) | 186, 804 |
| BseAI | TCCGGA | 1 cut(s) | 769 |
| BseDI | CCNNGG | 6 cut(s) | 97, 217, 352, 722, 785, 934 |
| BseGI | GGATG | 8 cut(s) | 638, 732, 853, 945, 1189, 1521, 1619, 1857 |
| BseJI | GATNNNNATC | 2 cut(s) | 186, 804 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 454, 560, 776, 935, 1128, 1815, 1816, 1906 |
| BseMI | GCAATG | 2 cut(s) | 1012, 1803 |
| BseMII | CTCAG | 2 cut(s) | 800, 1195 |
| BseNI | ACTGG | 4 cut(s) | 1280, 1740, 1817, 1853 |
| BseSI | GKGCMC | 1 cut(s) | 1352 |
| BseX3I | CGGCCG | 1 cut(s) | 492 |
| BseXI | GCAGC | 4 cut(s) | 28, 198, 1017, 1189 |
| BseYI | CCCAGC | 1 cut(s) | 1933 |
| BsgI | GTGCAG | 1 cut(s) | 35 |
| Bsh1236I | CGCG | 1 cut(s) | 1604 |
| Bsh1285I | CGRYCG | 2 cut(s) | 168, 495 |
| BshFI | GGCC | 2 cut(s) | 494, 691 |
| BshNI | GGYRCC | 2 cut(s) | 703, 911 |
| BsiEI | CGRYCG | 2 cut(s) | 168, 495 |
| BsiHKCI | CYCGRG | 2 cut(s) | 629, 1892 |
| BsiSI | CCGG | 9 cut(s) | 11, 99, 374, 692, 770, 777, 785, 1561, 1703 |
| BslFI | GGGAC | 2 cut(s) | 857, 1111 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 454, 560, 776, 935, 1128, 1815, 1816, 1906 |
| BsmAI | GTCTC | 5 cut(s) | 149, 800, 805, 1566, 1741 |
| BsmFI | GGGAC | 2 cut(s) | 857, 1111 |
| BsnI | GGCC | 2 cut(s) | 494, 691 |
| Bso31I | GGTCTC | 1 cut(s) | 805 |
| BsoBI | CYCGRG | 2 cut(s) | 629, 1892 |
| Bsp1286I | GDGCHC | 3 cut(s) | 636, 777, 1352 |
| Bsp13I | TCCGGA | 1 cut(s) | 769 |
| Bsp1407I | TGTACA | 1 cut(s) | 474 |
| Bsp143I | GATC | 6 cut(s) | 268, 291, 334, 1290, 1402, 1888 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1181 |
| Bsp19I | CCATGG | 1 cut(s) | 722 |
| BspACI | CCGC | 6 cut(s) | 469, 1072, 1205, 1602, 1691, 1930 |
| BspANI | GGCC | 2 cut(s) | 494, 691 |
| BspCNI | CTCAG | 2 cut(s) | 801, 1194 |
| BspEI | TCCGGA | 1 cut(s) | 769 |
| BspFNI | CGCG | 1 cut(s) | 1604 |
| BspLI | GGNNCC | 4 cut(s) | 705, 774, 913, 1099 |
| BspMAI | CTGCAG | 1 cut(s) | 412 |
| BspPI | GGATC | 2 cut(s) | 286, 329 |
| BspQI | GCTCTTC | 1 cut(s) | 819 |
| BspT107I | GGYRCC | 2 cut(s) | 703, 911 |
| BspTNI | GGTCTC | 1 cut(s) | 805 |
| BsrBI | CCGCTC | 1 cut(s) | 469 |
| BsrDI | GCAATG | 2 cut(s) | 1012, 1803 |
| BsrFI | RCCGGY | 2 cut(s) | 691, 1702 |
| BsrGI | TGTACA | 1 cut(s) | 474 |
| BsrI | ACTGG | 4 cut(s) | 1280, 1740, 1817, 1853 |
| BssAI | RCCGGY | 2 cut(s) | 691, 1702 |
| BssECI | CCNNGG | 6 cut(s) | 97, 217, 352, 722, 785, 934 |
| BssMI | GATC | 6 cut(s) | 268, 291, 334, 1290, 1402, 1888 |
| BssSI | CACGAG | 2 cut(s) | 878, 1640 |
| BssT1I | CCWWGG | 2 cut(s) | 352, 722 |
| Bst2BI | CACGAG | 2 cut(s) | 878, 1640 |
| Bst6I | CTCTTC | 2 cut(s) | 326, 819 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 1014, 1634, 1699 |
| BstAUI | TGTACA | 1 cut(s) | 474 |
| BstBAI | YACGTR | 1 cut(s) | 1941 |
| BstC8I | GCNNGC | 5 cut(s) | 72, 301, 412, 513, 1396 |
| BstDEI | CTNAG | 5 cut(s) | 26, 809, 989, 1181, 1389 |
| BstDSI | CCRYGG | 3 cut(s) | 217, 722, 934 |
| BstEII | GGTNACC | 1 cut(s) | 1037 |
| BstF5I | GGATG | 8 cut(s) | 638, 732, 853, 945, 1189, 1521, 1619, 1857 |
| BstFNI | CGCG | 1 cut(s) | 1604 |
| BstHHI | GCGC | 2 cut(s) | 1606, 1639 |
| BstKTI | GATC | 6 cut(s) | 271, 294, 337, 1293, 1405, 1891 |
| BstMAI | GTCTC | 5 cut(s) | 149, 800, 805, 1566, 1741 |
| BstMBI | GATC | 6 cut(s) | 268, 291, 334, 1290, 1402, 1888 |
| BstMCI | CGRYCG | 2 cut(s) | 168, 495 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 1014, 1634, 1699 |
| BstNSI | RCATGY | 4 cut(s) | 303, 372, 481, 899 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 369, 849 |
| BstPI | GGTNACC | 1 cut(s) | 1037 |
| BstSCI | CCNGG | 5 cut(s) | 9, 97, 775, 784, 1559 |
| BstSFI | CTRYAG | 2 cut(s) | 191, 408 |
| BstSLI | GKGCMC | 1 cut(s) | 1352 |
| BstSNI | TACGTA | 1 cut(s) | 1941 |
| BstUI | CGCG | 1 cut(s) | 1604 |
| BstV1I | GCAGC | 4 cut(s) | 28, 198, 1017, 1189 |
| BstV2I | GAAGAC | 6 cut(s) | 116, 221, 474, 665, 1379, 1432 |
| BstX2I | RGATCY | 3 cut(s) | 268, 291, 334 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1201 |
| BstYI | RGATCY | 3 cut(s) | 268, 291, 334 |
| BstZI | CGGCCG | 1 cut(s) | 492 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1389 |
| BsuI | GTATCC | 3 cut(s) | 269, 959, 1542 |
| BsuRI | GGCC | 2 cut(s) | 494, 691 |
| BtgI | CCRYGG | 3 cut(s) | 217, 722, 934 |
| BtgZI | GCGATG | 1 cut(s) | 1008 |
| BtsCI | GGATG | 8 cut(s) | 638, 732, 853, 945, 1189, 1521, 1619, 1857 |
| BtsI | GCAGTG | 3 cut(s) | 39, 198, 1158 |
| Cac8I | GCNNGC | 5 cut(s) | 72, 301, 412, 513, 1396 |
| CfoI | GCGC | 2 cut(s) | 1606, 1639 |
| Cfr10I | RCCGGY | 2 cut(s) | 691, 1702 |
| Cfr13I | GGNCC | 4 cut(s) | 250, 613, 1098, 1385 |
| CseI | GACGC | 3 cut(s) | 374, 594, 1636 |
| DdeI | CTNAG | 5 cut(s) | 26, 809, 989, 1181, 1389 |
| DpnI | GATC | 6 cut(s) | 270, 293, 336, 1292, 1404, 1890 |
| DpnII | GATC | 6 cut(s) | 268, 291, 334, 1290, 1402, 1888 |
| DraIII | CACNNNGTG | 1 cut(s) | 1114 |
| EaeI | YGGCCR | 2 cut(s) | 492, 689 |
| EagI | CGGCCG | 1 cut(s) | 492 |
| Eam1104I | CTCTTC | 2 cut(s) | 326, 819 |
| EarI | CTCTTC | 2 cut(s) | 326, 819 |
| EclXI | CGGCCG | 1 cut(s) | 492 |
| Eco105I | TACGTA | 1 cut(s) | 1941 |
| Eco130I | CCWWGG | 2 cut(s) | 352, 722 |
| Eco24I | GRGCYC | 2 cut(s) | 636, 777 |
| Eco31I | GGTCTC | 1 cut(s) | 805 |
| Eco47I | GGWCC | 4 cut(s) | 250, 613, 1098, 1385 |
| Eco52I | CGGCCG | 1 cut(s) | 492 |
| Eco57I | CTGAAG | 3 cut(s) | 1521, 1629, 1644 |
| Eco81I | CCTNAGG | 1 cut(s) | 1389 |
| Eco88I | CYCGRG | 2 cut(s) | 629, 1892 |
| Eco91I | GGTNACC | 1 cut(s) | 1037 |
| EcoO65I | GGTNACC | 1 cut(s) | 1037 |
| EcoT14I | CCWWGG | 2 cut(s) | 352, 722 |
| EcoT22I | ATGCAT | 2 cut(s) | 301, 897 |
| EcoT38I | GRGCYC | 2 cut(s) | 636, 777 |
| ErhI | CCWWGG | 2 cut(s) | 352, 722 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 528, 560 |
| FaqI | GGGAC | 2 cut(s) | 857, 1111 |
| FbaI | TGATCA | 1 cut(s) | 1290 |
| Fnu4HI | GCNGC | 6 cut(s) | 17, 212, 1006, 1178, 1206, 1931 |
| FokI | GGATG | 8 cut(s) | 625, 739, 840, 952, 1176, 1528, 1626, 1844 |
| FriOI | GRGCYC | 2 cut(s) | 636, 777 |
| Fsp4HI | GCNGC | 6 cut(s) | 17, 212, 1006, 1178, 1206, 1931 |
| FspBI | CTAG | 2 cut(s) | 1304, 1799 |
| FspI | TGCGCA | 1 cut(s) | 1638 |
| GlaI | GCGC | 2 cut(s) | 1605, 1638 |
| GluI | GCNGC | 6 cut(s) | 17, 212, 1006, 1178, 1206, 1931 |
| GsaI | CCCAGC | 1 cut(s) | 1937 |
| GsuI | CTGGAG | 2 cut(s) | 1297, 1723 |
| HaeIII | GGCC | 2 cut(s) | 494, 691 |
| HapII | CCGG | 9 cut(s) | 11, 99, 374, 692, 770, 777, 785, 1561, 1703 |
| HgaI | GACGC | 3 cut(s) | 374, 594, 1636 |
| HhaI | GCGC | 2 cut(s) | 1606, 1639 |
| Hin6I | GCGC | 2 cut(s) | 1604, 1637 |
| HinP1I | GCGC | 2 cut(s) | 1604, 1637 |
| HinfI | GANTC | 4 cut(s) | 403, 670, 766, 1772 |
| HpaII | CCGG | 9 cut(s) | 11, 99, 374, 692, 770, 777, 785, 1561, 1703 |
| HphI | GGTGA | 4 cut(s) | 706, 1031, 1642, 1898 |
| Hpy166II | GTNNAC | 4 cut(s) | 37, 94, 198, 873 |
| Hpy188I | TCNGA | 7 cut(s) | 108, 254, 610, 617, 643, 810, 1771 |
| Hpy8I | GTNNAC | 4 cut(s) | 37, 94, 198, 873 |
| Hpy99I | CGWCG | 1 cut(s) | 647 |
| HpyCH4IV | ACGT | 4 cut(s) | 135, 846, 954, 1940 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 1014, 1634, 1699 |
| HpyF3I | CTNAG | 5 cut(s) | 26, 809, 989, 1181, 1389 |
| HpySE526I | ACGT | 4 cut(s) | 135, 846, 954, 1940 |
| HspAI | GCGC | 2 cut(s) | 1604, 1637 |
| Kpn2I | TCCGGA | 1 cut(s) | 769 |
| KpnI | GGTACC | 2 cut(s) | 707, 915 |
| Ksp22I | TGATCA | 1 cut(s) | 1290 |
| Kzo9I | GATC | 6 cut(s) | 268, 291, 334, 1290, 1402, 1888 |
| LguI | GCTCTTC | 1 cut(s) | 819 |
| LmnI | GCTCC | 5 cut(s) | 466, 772, 1002, 1474, 1673 |
| Lsp1109I | GCAGC | 4 cut(s) | 28, 198, 1017, 1189 |
| LweI | GCATC | 3 cut(s) | 198, 882, 1302 |
| MaeI | CTAG | 2 cut(s) | 1304, 1799 |
| MaeII | ACGT | 4 cut(s) | 135, 846, 954, 1940 |
| MaeIII | GTNAC | 3 cut(s) | 380, 1037, 1648 |
| MalI | GATC | 6 cut(s) | 270, 293, 336, 1292, 1404, 1890 |
| MbiI | CCGCTC | 1 cut(s) | 469 |
| MboI | GATC | 6 cut(s) | 268, 291, 334, 1290, 1402, 1888 |
| MflI | RGATCY | 3 cut(s) | 268, 291, 334 |
| MhlI | GDGCHC | 3 cut(s) | 636, 777, 1352 |
| MluCI | AATT | 7 cut(s) | 524, 833, 1143, 1235, 1418, 1503, 1761 |
| MlyI | GAGTC | 3 cut(s) | 397, 679, 1766 |
| MmeI | TCCRAC | 3 cut(s) | 103, 444, 666 |
| MnlI | CCTC | 9 cut(s) | 447, 559, 604, 606, 618, 991, 1128, 1360, 1473 |
| Mph1103I | ATGCAT | 2 cut(s) | 301, 897 |
| MroI | TCCGGA | 1 cut(s) | 769 |
| MseI | TTAA | 5 cut(s) | 147, 831, 1158, 1421, 1827 |
| MslI | CAYNNNNRTG | 2 cut(s) | 727, 1655 |
| MspI | CCGG | 9 cut(s) | 11, 99, 374, 692, 770, 777, 785, 1561, 1703 |
| MspR9I | CCNGG | 5 cut(s) | 11, 99, 777, 786, 1561 |
| MvnI | CGCG | 1 cut(s) | 1604 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 1014, 1634, 1699 |
| NciI | CCSGG | 5 cut(s) | 11, 99, 777, 786, 1561 |
| NcoI | CCATGG | 1 cut(s) | 722 |
| NdeII | GATC | 6 cut(s) | 268, 291, 334, 1290, 1402, 1888 |
| NlaIV | GGNNCC | 4 cut(s) | 705, 774, 913, 1099 |
| NmuCI | GTSAC | 3 cut(s) | 380, 1037, 1648 |
| NsbI | TGCGCA | 1 cut(s) | 1638 |
| NsiI | ATGCAT | 2 cut(s) | 301, 897 |
| NspI | RCATGY | 4 cut(s) | 303, 372, 481, 899 |
| PaeI | GCATGC | 1 cut(s) | 303 |
| PaeR7I | CTCGAG | 1 cut(s) | 1892 |
| PciI | ACATGT | 1 cut(s) | 477 |
| PciSI | GCTCTTC | 1 cut(s) | 819 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 250 |
| PfeI | GAWTC | 1 cut(s) | 766 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1128 |
| PkrI | GCNGC | 6 cut(s) | 18, 213, 1007, 1179, 1207, 1932 |
| PleI | GAGTC | 3 cut(s) | 397, 678, 1766 |
| PpsI | GAGTC | 3 cut(s) | 397, 678, 1766 |
| Ppu21I | YACGTR | 1 cut(s) | 1941 |
| PscI | ACATGT | 1 cut(s) | 477 |
| PshAI | GACNNNNGTC | 2 cut(s) | 369, 849 |
| Psp1406I | AACGTT | 1 cut(s) | 954 |
| PspEI | GGTNACC | 1 cut(s) | 1037 |
| PspFI | CCCAGC | 1 cut(s) | 1933 |
| PspN4I | GGNNCC | 4 cut(s) | 705, 774, 913, 1099 |
| PspPI | GGNCC | 4 cut(s) | 250, 613, 1098, 1385 |
| PstI | CTGCAG | 1 cut(s) | 412 |
| PsuI | RGATCY | 3 cut(s) | 268, 291, 334 |
| RseI | CAYNNNNRTG | 2 cut(s) | 727, 1655 |
| SapI | GCTCTTC | 1 cut(s) | 819 |
| SaqAI | TTAA | 5 cut(s) | 147, 831, 1158, 1421, 1827 |
| SatI | GCNGC | 6 cut(s) | 17, 212, 1006, 1178, 1206, 1931 |
| Sau3AI | GATC | 6 cut(s) | 268, 291, 334, 1290, 1402, 1888 |
| Sau96I | GGNCC | 4 cut(s) | 250, 613, 1098, 1385 |
| SbfI | CCTGCAGG | 1 cut(s) | 412 |
| SchI | GAGTC | 3 cut(s) | 397, 679, 1766 |
| ScrFI | CCNGG | 5 cut(s) | 11, 99, 777, 786, 1561 |
| SdaI | CCTGCAGG | 1 cut(s) | 412 |
| SduI | GDGCHC | 3 cut(s) | 636, 777, 1352 |
| SfaNI | GCATC | 3 cut(s) | 198, 882, 1302 |
| SfcI | CTRYAG | 2 cut(s) | 191, 408 |
| Sfr274I | CTCGAG | 1 cut(s) | 1892 |
| SinI | GGWCC | 4 cut(s) | 250, 613, 1098, 1385 |
| SlaI | CTCGAG | 1 cut(s) | 1892 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 727, 1655 |
| SmlI | CTYRAG | 2 cut(s) | 1464, 1892 |
| SmoI | CTYRAG | 2 cut(s) | 1464, 1892 |
| SnaBI | TACGTA | 1 cut(s) | 1941 |
| SphI | GCATGC | 1 cut(s) | 303 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 412 |
| Sse9I | AATT | 7 cut(s) | 524, 833, 1143, 1235, 1418, 1503, 1761 |
| SsiI | CCGC | 6 cut(s) | 469, 1072, 1205, 1602, 1691, 1930 |
| SspMI | CTAG | 2 cut(s) | 1304, 1799 |
| StyD4I | CCNGG | 5 cut(s) | 9, 97, 775, 784, 1559 |
| StyI | CCWWGG | 2 cut(s) | 352, 722 |
| TaiI | ACGT | 4 cut(s) | 138, 849, 957, 1943 |
| TaqI | TCGA | 5 cut(s) | 164, 206, 798, 1077, 1893 |
| TasI | AATT | 7 cut(s) | 524, 833, 1143, 1235, 1418, 1503, 1761 |
| TatI | WGTACW | 4 cut(s) | 57, 195, 474, 870 |
| TauI | GCSGC | 2 cut(s) | 1208, 1933 |
| TfiI | GAWTC | 1 cut(s) | 766 |
| Tru1I | TTAA | 5 cut(s) | 147, 831, 1158, 1421, 1827 |
| Tru9I | TTAA | 5 cut(s) | 147, 831, 1158, 1421, 1827 |
| TseFI | GTSAC | 3 cut(s) | 380, 1037, 1648 |
| TseI | GCWGC | 4 cut(s) | 16, 211, 1005, 1177 |
| Tsp45I | GTSAC | 3 cut(s) | 380, 1037, 1648 |
| TspDTI | ATGAA | 2 cut(s) | 749, 1673 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1128 |
| VpaK11BI | GGWCC | 4 cut(s) | 250, 613, 1098, 1385 |
| XapI | RAATTY | 4 cut(s) | 833, 1143, 1235, 1761 |
| XceI | RCATGY | 4 cut(s) | 303, 372, 481, 899 |
| XhoI | CTCGAG | 1 cut(s) | 1892 |
| XspI | CTAG | 2 cut(s) | 1304, 1799 |
| Zsp2I | ATGCAT | 2 cut(s) | 301, 897 |