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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 963 |
| Acc65I | GGTACC | 2 cut(s) | 496, 1598 |
| AccB1I | GGYRCC | 3 cut(s) | 496, 1598, 2328 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 854, 1391 |
| AccI | GTMKAC | 1 cut(s) | 91 |
| AciI | CCGC | 2 cut(s) | 799, 1764 |
| AclWI | GGATC | 1 cut(s) | 2253 |
| AcoI | YGGCCR | 2 cut(s) | 845, 941 |
| AcsI | RAATTY | 4 cut(s) | 46, 128, 558, 851 |
| AcuI | CTGAAG | 2 cut(s) | 128, 1103 |
| AcvI | CACGTG | 1 cut(s) | 1972 |
| AdeI | CACNNNGTG | 2 cut(s) | 843, 1876 |
| AfaI | GTAC | 7 cut(s) | 370, 498, 634, 701, 1542, 1600, 1886 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 186, 765, 798, 854, 1187, 1391, 1894, 2261 |
| AflII | CTTAAG | 1 cut(s) | 1853 |
| AjnI | CCWGG | 3 cut(s) | 218, 821, 1615 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 120, 152, 1631, 1663 |
| AluBI | AGCT | 8 cut(s) | 8, 353, 611, 665, 1445, 2441, 2535, 2571 |
| AluI | AGCT | 8 cut(s) | 8, 353, 611, 665, 1445, 2441, 2535, 2571 |
| Alw21I | GWGCWC | 1 cut(s) | 1938 |
| Alw26I | GTCTC | 2 cut(s) | 1828, 2166 |
| AlwI | GGATC | 1 cut(s) | 2253 |
| Ama87I | CYCGRG | 2 cut(s) | 1037, 1198 |
| AoxI | GGCC | 5 cut(s) | 151, 756, 845, 941, 2101 |
| ApeKI | GCWGC | 7 cut(s) | 74, 924, 1340, 1343, 1738, 2096, 2129 |
| ApoI | RAATTY | 4 cut(s) | 46, 128, 558, 851 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 558, 2013 |
| Asp718I | GGTACC | 2 cut(s) | 496, 1598 |
| AspS9I | GGNCC | 7 cut(s) | 151, 471, 598, 1025, 1164, 1767, 2102 |
| AsuC2I | CCSGG | 4 cut(s) | 363, 1038, 1039, 1895 |
| AsuNHI | GCTAGC | 2 cut(s) | 4, 2567 |
| AvaI | CYCGRG | 2 cut(s) | 1037, 1198 |
| AvaII | GGWCC | 5 cut(s) | 471, 598, 1025, 1164, 1767 |
| AxyI | CCTNAGG | 1 cut(s) | 264 |
| BaeGI | GKGCMC | 1 cut(s) | 2333 |
| BalI | TGGCCA | 1 cut(s) | 847 |
| BanI | GGYRCC | 3 cut(s) | 496, 1598, 2328 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1943, 1975 |
| BauI | CACGAG | 1 cut(s) | 1428 |
| BbrPI | CACGTG | 1 cut(s) | 1972 |
| BbsI | GAAGAC | 3 cut(s) | 279, 1498, 1628 |
| Bbv12I | GWGCWC | 1 cut(s) | 1938 |
| BbvI | GCAGC | 7 cut(s) | 61, 936, 1327, 1330, 1725, 2083, 2116 |
| BceAI | ACGGC | 1 cut(s) | 1530 |
| BciT130I | CCWGG | 3 cut(s) | 220, 823, 1617 |
| BciVI | GTATCC | 1 cut(s) | 369 |
| BcnI | CCSGG | 4 cut(s) | 363, 1038, 1039, 1895 |
| BcoDI | GTCTC | 2 cut(s) | 1828, 2166 |
| BfaI | CTAG | 5 cut(s) | 5, 1820, 2300, 2454, 2568 |
| BfmI | CTRYAG | 1 cut(s) | 1791 |
| BfrI | CTTAAG | 1 cut(s) | 1853 |
| BfuI | GTATCC | 1 cut(s) | 369 |
| BglII | AGATCT | 2 cut(s) | 17, 615 |
| BisI | GCNGC | 8 cut(s) | 75, 800, 925, 1341, 1344, 1739, 2097, 2130 |
| BlpI | GCTNAGC | 1 cut(s) | 880 |
| BlsI | GCNGC | 8 cut(s) | 76, 801, 926, 1342, 1345, 1740, 2098, 2131 |
| Bme1390I | CCNGG | 7 cut(s) | 220, 363, 823, 1038, 1039, 1617, 1895 |
| Bme18I | GGWCC | 5 cut(s) | 471, 598, 1025, 1164, 1767 |
| BmeT110I | CYCGRG | 2 cut(s) | 1037, 1198 |
| BmgT120I | GGNCC | 7 cut(s) | 151, 471, 598, 1025, 1164, 1767, 2102 |
| BmiI | GGNNCC | 9 cut(s) | 472, 498, 599, 872, 1193, 1600, 1768, 1817, 2330 |
| BmrFI | CCNGG | 7 cut(s) | 220, 363, 823, 1038, 1039, 1617, 1895 |
| BmrI | ACTGGG | 1 cut(s) | 45 |
| BmsI | GCATC | 8 cut(s) | 778, 786, 811, 993, 1108, 1576, 1677, 1869 |
| BmtI | GCTAGC | 2 cut(s) | 8, 2571 |
| BmuI | ACTGGG | 1 cut(s) | 45 |
| BpiI | GAAGAC | 3 cut(s) | 279, 1498, 1628 |
| BpmI | CTGGAG | 1 cut(s) | 1413 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 880 |
| BpuMI | CCSGG | 4 cut(s) | 363, 1038, 1039, 1895 |
| Bsa29I | ATCGAT | 1 cut(s) | 1050 |
| BsaAI | YACGTR | 1 cut(s) | 1972 |
| BsaBI | GATNNNNATC | 2 cut(s) | 1056, 2250 |
| BsaJI | CCNNGG | 7 cut(s) | 219, 362, 974, 1037, 1616, 1626, 2347 |
| BsaWI | WCCGGW | 1 cut(s) | 481 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1386, 1416 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 186, 765, 798, 854, 1187, 1391, 1894, 2261 |
| Bse118I | RCCGGY | 2 cut(s) | 943, 2099 |
| Bse1I | ACTGG | 6 cut(s) | 40, 458, 736, 1396, 1538, 2027 |
| Bse21I | CCTNAGG | 1 cut(s) | 264 |
| Bse3DI | GCAATG | 2 cut(s) | 2533, 2560 |
| Bse8I | GATNNNNATC | 2 cut(s) | 1056, 2250 |
| BseBI | CCWGG | 3 cut(s) | 220, 823, 1617 |
| BseCI | ATCGAT | 1 cut(s) | 1050 |
| BseDI | CCNNGG | 7 cut(s) | 219, 362, 974, 1037, 1616, 1626, 2347 |
| BseGI | GGATG | 9 cut(s) | 490, 549, 735, 802, 1321, 1375, 1636, 1995, 2377 |
| BseJI | GATNNNNATC | 2 cut(s) | 1056, 2250 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 186, 765, 798, 854, 1187, 1391, 1894, 2261 |
| BseMI | GCAATG | 2 cut(s) | 2533, 2560 |
| BseMII | CTCAG | 5 cut(s) | 255, 327, 603, 1136, 2433 |
| BseNI | ACTGG | 6 cut(s) | 40, 458, 736, 1396, 1538, 2027 |
| BseRI | GAGGAG | 4 cut(s) | 311, 1143, 1309, 2399 |
| BseSI | GKGCMC | 1 cut(s) | 2333 |
| BseXI | GCAGC | 7 cut(s) | 61, 936, 1327, 1330, 1725, 2083, 2116 |
| BseYI | CCCAGC | 1 cut(s) | 1096 |
| BshFI | GGCC | 5 cut(s) | 153, 758, 847, 943, 2103 |
| BshNI | GGYRCC | 3 cut(s) | 496, 1598, 2328 |
| BshVI | ATCGAT | 1 cut(s) | 1050 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1938 |
| BsiHKCI | CYCGRG | 2 cut(s) | 1037, 1198 |
| BsiSI | CCGG | 6 cut(s) | 362, 482, 944, 1038, 1895, 2100 |
| BslFI | GGGAC | 2 cut(s) | 611, 686 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 186, 765, 798, 854, 1187, 1391, 1894, 2261 |
| BsmAI | GTCTC | 2 cut(s) | 1828, 2166 |
| BsmFI | GGGAC | 2 cut(s) | 611, 686 |
| BsmI | GAATGC | 3 cut(s) | 1346, 1645, 2569 |
| BsnI | GGCC | 5 cut(s) | 153, 758, 847, 943, 2103 |
| BsoBI | CYCGRG | 2 cut(s) | 1037, 1198 |
| Bsp1286I | GDGCHC | 2 cut(s) | 1938, 2333 |
| Bsp1407I | TGTACA | 1 cut(s) | 368 |
| Bsp143I | GATC | 7 cut(s) | 17, 615, 831, 1078, 1463, 1907, 2245 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 880 |
| BspACI | CCGC | 2 cut(s) | 799, 1764 |
| BspANI | GGCC | 5 cut(s) | 153, 758, 847, 943, 2103 |
| BspCNI | CTCAG | 5 cut(s) | 256, 326, 604, 1137, 2434 |
| BspDI | ATCGAT | 1 cut(s) | 1050 |
| BspHI | TCATGA | 1 cut(s) | 2545 |
| BspLI | GGNNCC | 9 cut(s) | 472, 498, 599, 872, 1193, 1600, 1768, 1817, 2330 |
| BspOI | GCTAGC | 2 cut(s) | 8, 2571 |
| BspPI | GGATC | 1 cut(s) | 2253 |
| BspT107I | GGYRCC | 3 cut(s) | 496, 1598, 2328 |
| BspTI | CTTAAG | 1 cut(s) | 1853 |
| BsrDI | GCAATG | 2 cut(s) | 2533, 2560 |
| BsrFI | RCCGGY | 2 cut(s) | 943, 2099 |
| BsrGI | TGTACA | 1 cut(s) | 368 |
| BsrI | ACTGG | 6 cut(s) | 40, 458, 736, 1396, 1538, 2027 |
| BssAI | RCCGGY | 2 cut(s) | 943, 2099 |
| BssECI | CCNNGG | 7 cut(s) | 219, 362, 974, 1037, 1616, 1626, 2347 |
| BssMI | GATC | 7 cut(s) | 17, 615, 831, 1078, 1463, 1907, 2245 |
| BssNAI | GTATAC | 1 cut(s) | 92 |
| BssSI | CACGAG | 1 cut(s) | 1428 |
| BssT1I | CCWWGG | 2 cut(s) | 974, 1626 |
| Bst1107I | GTATAC | 1 cut(s) | 92 |
| Bst2BI | CACGAG | 1 cut(s) | 1428 |
| Bst2UI | CCWGG | 3 cut(s) | 220, 823, 1617 |
| Bst4CI | ACNGT | 9 cut(s) | 278, 581, 1068, 1876, 2083, 2264, 2348, 2397, 2501 |
| Bst6I | CTCTTC | 3 cut(s) | 234, 983, 1392 |
| BstAFI | CTTAAG | 1 cut(s) | 1853 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 74 |
| BstAUI | TGTACA | 1 cut(s) | 368 |
| BstBAI | YACGTR | 1 cut(s) | 1972 |
| BstC8I | GCNNGC | 8 cut(s) | 6, 771, 1182, 1338, 1455, 2101, 2537, 2569 |
| BstDEI | CTNAG | 7 cut(s) | 264, 313, 612, 880, 1145, 2380, 2442 |
| BstDSI | CCRYGG | 1 cut(s) | 2347 |
| BstF5I | GGATG | 9 cut(s) | 490, 549, 735, 802, 1321, 1375, 1636, 1995, 2377 |
| BstKTI | GATC | 7 cut(s) | 20, 618, 834, 1081, 1466, 1910, 2248 |
| BstMAI | GTCTC | 2 cut(s) | 1828, 2166 |
| BstMBI | GATC | 7 cut(s) | 17, 615, 831, 1078, 1463, 1907, 2245 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 74, 1459, 2532 |
| BstNI | CCWGG | 3 cut(s) | 220, 823, 1617 |
| BstNSI | RCATGY | 4 cut(s) | 570, 773, 1340, 1783 |
| BstSCI | CCNGG | 7 cut(s) | 218, 361, 821, 1036, 1037, 1615, 1893 |
| BstSFI | CTRYAG | 1 cut(s) | 1791 |
| BstSLI | GKGCMC | 1 cut(s) | 2333 |
| BstV1I | GCAGC | 7 cut(s) | 61, 936, 1327, 1330, 1725, 2083, 2116 |
| BstV2I | GAAGAC | 3 cut(s) | 279, 1498, 1628 |
| BstX2I | RGATCY | 2 cut(s) | 17, 615 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 2144 |
| BstYI | RGATCY | 2 cut(s) | 17, 615 |
| BstZ17I | GTATAC | 1 cut(s) | 92 |
| Bsu15I | ATCGAT | 1 cut(s) | 1050 |
| Bsu36I | CCTNAGG | 1 cut(s) | 264 |
| BsuI | GTATCC | 1 cut(s) | 369 |
| BsuRI | GGCC | 5 cut(s) | 153, 758, 847, 943, 2103 |
| BsuTUI | ATCGAT | 1 cut(s) | 1050 |
| BtgI | CCRYGG | 1 cut(s) | 2347 |
| BtgZI | GCGATG | 1 cut(s) | 1599 |
| BtsCI | GGATG | 9 cut(s) | 490, 549, 735, 802, 1321, 1375, 1636, 1995, 2377 |
| BtsIMutI | CAGTG | 2 cut(s) | 1142, 1881 |
| Cac8I | GCNNGC | 8 cut(s) | 6, 771, 1182, 1338, 1455, 2101, 2537, 2569 |
| CciI | TCATGA | 1 cut(s) | 2545 |
| Cfr10I | RCCGGY | 2 cut(s) | 943, 2099 |
| Cfr13I | GGNCC | 7 cut(s) | 151, 471, 598, 1025, 1164, 1767, 2102 |
| Cfr9I | CCCGGG | 1 cut(s) | 1037 |
| ClaI | ATCGAT | 1 cut(s) | 1050 |
| CseI | GACGC | 1 cut(s) | 2312 |
| Csp6I | GTAC | 7 cut(s) | 369, 497, 633, 700, 1541, 1599, 1885 |
| CviQI | GTAC | 7 cut(s) | 369, 497, 633, 700, 1541, 1599, 1885 |
| DdeI | CTNAG | 7 cut(s) | 264, 313, 612, 880, 1145, 2380, 2442 |
| DpnI | GATC | 7 cut(s) | 19, 617, 833, 1080, 1465, 1909, 2247 |
| DpnII | GATC | 7 cut(s) | 17, 615, 831, 1078, 1463, 1907, 2245 |
| DraI | TTTAAA | 2 cut(s) | 510, 1839 |
| DraIII | CACNNNGTG | 2 cut(s) | 843, 1876 |
| EaeI | YGGCCR | 2 cut(s) | 845, 941 |
| Eam1104I | CTCTTC | 3 cut(s) | 234, 983, 1392 |
| EarI | CTCTTC | 3 cut(s) | 234, 983, 1392 |
| Eco130I | CCWWGG | 2 cut(s) | 974, 1626 |
| Eco147I | AGGCCT | 1 cut(s) | 758 |
| Eco32I | GATATC | 1 cut(s) | 1048 |
| Eco47I | GGWCC | 5 cut(s) | 471, 598, 1025, 1164, 1767 |
| Eco57I | CTGAAG | 2 cut(s) | 128, 1103 |
| Eco72I | CACGTG | 1 cut(s) | 1972 |
| Eco81I | CCTNAGG | 1 cut(s) | 264 |
| Eco88I | CYCGRG | 2 cut(s) | 1037, 1198 |
| EcoO109I | RGGNCCY | 2 cut(s) | 471, 1767 |
| EcoRI | GAATTC | 1 cut(s) | 128 |
| EcoRII | CCWGG | 3 cut(s) | 218, 821, 1615 |
| EcoRV | GATATC | 1 cut(s) | 1048 |
| EcoT14I | CCWWGG | 2 cut(s) | 974, 1626 |
| EcoT22I | ATGCAT | 2 cut(s) | 568, 986 |
| ErhI | CCWWGG | 2 cut(s) | 974, 1626 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1490, 1522, 1931, 1963 |
| FaqI | GGGAC | 2 cut(s) | 611, 686 |
| FauI | CCCGC | 1 cut(s) | 1757 |
| FauNDI | CATATG | 1 cut(s) | 2463 |
| FblI | GTMKAC | 1 cut(s) | 91 |
| Fnu4HI | GCNGC | 8 cut(s) | 75, 800, 925, 1341, 1344, 1739, 2097, 2130 |
| FokI | GGATG | 9 cut(s) | 497, 556, 742, 789, 1308, 1382, 1643, 2002, 2384 |
| Fsp4HI | GCNGC | 8 cut(s) | 75, 800, 925, 1341, 1344, 1739, 2097, 2130 |
| FspBI | CTAG | 5 cut(s) | 5, 1820, 2300, 2454, 2568 |
| GluI | GCNGC | 8 cut(s) | 75, 800, 925, 1341, 1344, 1739, 2097, 2130 |
| GsaI | CCCAGC | 1 cut(s) | 1100 |
| GsuI | CTGGAG | 1 cut(s) | 1413 |
| HaeIII | GGCC | 5 cut(s) | 153, 758, 847, 943, 2103 |
| HapII | CCGG | 6 cut(s) | 362, 482, 944, 1038, 1895, 2100 |
| HgaI | GACGC | 1 cut(s) | 2312 |
| HincII | GTYRAC | 3 cut(s) | 378, 2152, 2422 |
| HindII | GTYRAC | 3 cut(s) | 378, 2152, 2422 |
| HindIII | AAGCTT | 1 cut(s) | 351 |
| HinfI | GANTC | 7 cut(s) | 242, 248, 311, 720, 812, 900, 1297 |
| HpaII | CCGG | 6 cut(s) | 362, 482, 944, 1038, 1895, 2100 |
| Hpy166II | GTNNAC | 7 cut(s) | 92, 369, 378, 2152, 2272, 2422, 2459 |
| Hpy188I | TCNGA | 8 cut(s) | 108, 247, 316, 451, 895, 1083, 2193, 2260 |
| Hpy188III | TCNNGA | 9 cut(s) | 1000, 1023, 1430, 1553, 1606, 1825, 2018, 2300, 2546 |
| Hpy8I | GTNNAC | 7 cut(s) | 92, 369, 378, 2152, 2272, 2422, 2459 |
| HpyAV | CCTTC | 9 cut(s) | 218, 748, 884, 1326, 1360, 1412, 1898, 2474, 2516 |
| HpyCH4III | ACNGT | 9 cut(s) | 278, 581, 1068, 1876, 2083, 2264, 2348, 2397, 2501 |
| HpyCH4IV | ACGT | 5 cut(s) | 94, 912, 1270, 1971, 2178 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 74, 1459, 2532 |
| HpyF3I | CTNAG | 7 cut(s) | 264, 313, 612, 880, 1145, 2380, 2442 |
| HpySE526I | ACGT | 5 cut(s) | 94, 912, 1270, 1971, 2178 |
| KpnI | GGTACC | 2 cut(s) | 500, 1602 |
| KroI | GCCGGC | 1 cut(s) | 2099 |
| KroNI | GCCGGC | 1 cut(s) | 2101 |
| Kzo9I | GATC | 7 cut(s) | 17, 615, 831, 1078, 1463, 1907, 2245 |
| LmnI | GCTCC | 2 cut(s) | 662, 1933 |
| Lsp1109I | GCAGC | 7 cut(s) | 61, 936, 1327, 1330, 1725, 2083, 2116 |
| LweI | GCATC | 8 cut(s) | 778, 786, 811, 993, 1108, 1576, 1677, 1869 |
| MaeI | CTAG | 5 cut(s) | 5, 1820, 2300, 2454, 2568 |
| MaeII | ACGT | 5 cut(s) | 94, 912, 1270, 1971, 2178 |
| MaeIII | GTNAC | 9 cut(s) | 116, 195, 308, 477, 738, 1207, 1372, 1857, 2203 |
| MalI | GATC | 7 cut(s) | 19, 617, 833, 1080, 1465, 1909, 2247 |
| MboI | GATC | 7 cut(s) | 17, 615, 831, 1078, 1463, 1907, 2245 |
| MfeI | CAATTG | 1 cut(s) | 2048 |
| MflI | RGATCY | 2 cut(s) | 17, 615 |
| MhlI | GDGCHC | 2 cut(s) | 1938, 2333 |
| MlsI | TGGCCA | 1 cut(s) | 847 |
| MluNI | TGGCCA | 1 cut(s) | 847 |
| MlyI | GAGTC | 3 cut(s) | 251, 305, 1306 |
| MmeI | TCCRAC | 4 cut(s) | 474, 830, 1709, 2429 |
| Mox20I | TGGCCA | 1 cut(s) | 847 |
| Mph1103I | ATGCAT | 2 cut(s) | 568, 986 |
| MroNI | GCCGGC | 1 cut(s) | 2099 |
| MroXI | GAANNNNTTC | 2 cut(s) | 558, 2013 |
| MscI | TGGCCA | 1 cut(s) | 847 |
| MslI | CAYNNNNRTG | 4 cut(s) | 1877, 2142, 2517, 2547 |
| Msp20I | TGGCCA | 1 cut(s) | 847 |
| MspCI | CTTAAG | 1 cut(s) | 1853 |
| MspI | CCGG | 6 cut(s) | 362, 482, 944, 1038, 1895, 2100 |
| MspR9I | CCNGG | 7 cut(s) | 220, 363, 823, 1038, 1039, 1617, 1895 |
| MunI | CAATTG | 1 cut(s) | 2048 |
| Mva1269I | GAATGC | 3 cut(s) | 1346, 1645, 2569 |
| MvaI | CCWGG | 3 cut(s) | 220, 823, 1617 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 74, 1459, 2532 |
| NaeI | GCCGGC | 1 cut(s) | 2101 |
| NciI | CCSGG | 4 cut(s) | 363, 1038, 1039, 1895 |
| NdeI | CATATG | 1 cut(s) | 2463 |
| NdeII | GATC | 7 cut(s) | 17, 615, 831, 1078, 1463, 1907, 2245 |
| NgoMIV | GCCGGC | 1 cut(s) | 2099 |
| NheI | GCTAGC | 2 cut(s) | 4, 2567 |
| NlaIV | GGNNCC | 9 cut(s) | 472, 498, 599, 872, 1193, 1600, 1768, 1817, 2330 |
| NmuCI | GTSAC | 6 cut(s) | 195, 308, 738, 1207, 1372, 1857 |
| NsiI | ATGCAT | 2 cut(s) | 568, 986 |
| NspI | RCATGY | 4 cut(s) | 570, 773, 1340, 1783 |
| PaeI | GCATGC | 2 cut(s) | 773, 1340 |
| PagI | TCATGA | 1 cut(s) | 2545 |
| PceI | AGGCCT | 1 cut(s) | 758 |
| PctI | GAATGC | 3 cut(s) | 1346, 1645, 2569 |
| PdiI | GCCGGC | 1 cut(s) | 2101 |
| PdmI | GAANNNNTTC | 2 cut(s) | 558, 2013 |
| PfeI | GAWTC | 4 cut(s) | 248, 720, 812, 900 |
| PflFI | GACNNNGTC | 3 cut(s) | 193, 1655, 2177 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 854, 1391 |
| PkrI | GCNGC | 8 cut(s) | 76, 801, 926, 1342, 1345, 1740, 2098, 2131 |
| PleI | GAGTC | 3 cut(s) | 250, 305, 1305 |
| PmaCI | CACGTG | 1 cut(s) | 1972 |
| PmlI | CACGTG | 1 cut(s) | 1972 |
| PpsI | GAGTC | 3 cut(s) | 250, 305, 1305 |
| Ppu21I | YACGTR | 1 cut(s) | 1972 |
| PpuMI | RGGWCCY | 2 cut(s) | 471, 1767 |
| PsiI | TTATAA | 1 cut(s) | 963 |
| Psp5II | RGGWCCY | 2 cut(s) | 471, 1767 |
| Psp6I | CCWGG | 3 cut(s) | 218, 821, 1615 |
| PspCI | CACGTG | 1 cut(s) | 1972 |
| PspFI | CCCAGC | 1 cut(s) | 1096 |
| PspGI | CCWGG | 3 cut(s) | 218, 821, 1615 |
| PspN4I | GGNNCC | 9 cut(s) | 472, 498, 599, 872, 1193, 1600, 1768, 1817, 2330 |
| PspPI | GGNCC | 7 cut(s) | 151, 471, 598, 1025, 1164, 1767, 2102 |
| PspPPI | RGGWCCY | 2 cut(s) | 471, 1767 |
| PsuI | RGATCY | 2 cut(s) | 17, 615 |
| PsyI | GACNNNGTC | 3 cut(s) | 193, 1655, 2177 |
| RsaI | GTAC | 7 cut(s) | 370, 498, 634, 701, 1542, 1600, 1886 |
| RsaNI | GTAC | 7 cut(s) | 369, 497, 633, 700, 1541, 1599, 1885 |
| RseI | CAYNNNNRTG | 4 cut(s) | 1877, 2142, 2517, 2547 |
| SatI | GCNGC | 8 cut(s) | 75, 800, 925, 1341, 1344, 1739, 2097, 2130 |
| Sau3AI | GATC | 7 cut(s) | 17, 615, 831, 1078, 1463, 1907, 2245 |
| Sau96I | GGNCC | 7 cut(s) | 151, 471, 598, 1025, 1164, 1767, 2102 |
| SchI | GAGTC | 3 cut(s) | 251, 305, 1306 |
| ScrFI | CCNGG | 7 cut(s) | 220, 363, 823, 1038, 1039, 1617, 1895 |
| SduI | GDGCHC | 2 cut(s) | 1938, 2333 |
| SfaNI | GCATC | 8 cut(s) | 778, 786, 811, 993, 1108, 1576, 1677, 1869 |
| SfcI | CTRYAG | 1 cut(s) | 1791 |
| SinI | GGWCC | 5 cut(s) | 471, 598, 1025, 1164, 1767 |
| SmaI | CCCGGG | 1 cut(s) | 1039 |
| SmiI | ATTTAAAT | 1 cut(s) | 510 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 1877, 2142, 2517, 2547 |
| SmlI | CTYRAG | 1 cut(s) | 1853 |
| SmoI | CTYRAG | 1 cut(s) | 1853 |
| SphI | GCATGC | 2 cut(s) | 773, 1340 |
| SseBI | AGGCCT | 1 cut(s) | 758 |
| SsiI | CCGC | 2 cut(s) | 799, 1764 |
| SspI | AATATT | 3 cut(s) | 1136, 1612, 1810 |
| SspMI | CTAG | 5 cut(s) | 5, 1820, 2300, 2454, 2568 |
| StuI | AGGCCT | 1 cut(s) | 758 |
| StyD4I | CCNGG | 7 cut(s) | 218, 361, 821, 1036, 1037, 1615, 1893 |
| StyI | CCWWGG | 2 cut(s) | 974, 1626 |
| SwaI | ATTTAAAT | 1 cut(s) | 510 |
| TaaI | ACNGT | 9 cut(s) | 278, 581, 1068, 1876, 2083, 2264, 2348, 2397, 2501 |
| TaiI | ACGT | 5 cut(s) | 97, 915, 1273, 1974, 2181 |
| TaqI | TCGA | 5 cut(s) | 834, 1050, 1350, 1659, 1826 |
| TaqII | GACCGA | 1 cut(s) | 1042 |
| TatI | WGTACW | 2 cut(s) | 368, 632 |
| TauI | GCSGC | 1 cut(s) | 802 |
| TfiI | GAWTC | 4 cut(s) | 248, 720, 812, 900 |
| TscAI | CASTG | 2 cut(s) | 1149, 1881 |
| TseFI | GTSAC | 6 cut(s) | 195, 308, 738, 1207, 1372, 1857 |
| TseI | GCWGC | 7 cut(s) | 74, 924, 1340, 1343, 1738, 2096, 2129 |
| Tsp45I | GTSAC | 6 cut(s) | 195, 308, 738, 1207, 1372, 1857 |
| TspMI | CCCGGG | 1 cut(s) | 1037 |
| TspRI | CASTG | 2 cut(s) | 1149, 1881 |
| Tth111I | GACNNNGTC | 3 cut(s) | 193, 1655, 2177 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 854, 1391 |
| Vha464I | CTTAAG | 1 cut(s) | 1853 |
| VpaK11BI | GGWCC | 5 cut(s) | 471, 598, 1025, 1164, 1767 |
| XapI | RAATTY | 4 cut(s) | 46, 128, 558, 851 |
| XbaI | TCTAGA | 1 cut(s) | 2299 |
| XceI | RCATGY | 4 cut(s) | 570, 773, 1340, 1783 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 1485, 1717 |
| XmaI | CCCGGG | 1 cut(s) | 1037 |
| XmiI | GTMKAC | 1 cut(s) | 91 |
| XmnI | GAANNNNTTC | 2 cut(s) | 558, 2013 |
| XspI | CTAG | 5 cut(s) | 5, 1820, 2300, 2454, 2568 |
| Zsp2I | ATGCAT | 2 cut(s) | 568, 986 |