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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 2025, 2209 |
| AasI | GACNNNNNNGTC | 2 cut(s) | 544, 1073 |
| AccB1I | GGYRCC | 2 cut(s) | 1084, 1591 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 875 |
| AccI | GTMKAC | 1 cut(s) | 1637 |
| AciI | CCGC | 4 cut(s) | 830, 1260, 1579, 1629 |
| AclWI | GGATC | 2 cut(s) | 68, 1566 |
| AcoI | YGGCCR | 3 cut(s) | 866, 962, 2120 |
| AcsI | RAATTY | 6 cut(s) | 50, 473, 670, 1016, 1213, 1702 |
| AcuI | CTGAAG | 3 cut(s) | 1926, 1941, 2133 |
| AdeI | CACNNNGTG | 1 cut(s) | 437 |
| AfaI | GTAC | 2 cut(s) | 699, 1977 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 641, 875, 901, 1208, 1259, 1601 |
| AflIII | ACRYGT | 4 cut(s) | 112, 861, 1485, 1867 |
| AgsI | TTSAA | 7 cut(s) | 296, 427, 472, 516, 675, 1834, 2143 |
| AhlI | ACTAGT | 1 cut(s) | 1388 |
| AjnI | CCWGG | 2 cut(s) | 1057, 1522 |
| Alw26I | GTCTC | 1 cut(s) | 1148 |
| AlwI | GGATC | 2 cut(s) | 68, 1566 |
| AoxI | GGCC | 3 cut(s) | 866, 962, 2120 |
| ApeKI | GCWGC | 6 cut(s) | 68, 299, 1054, 1510, 1547, 1845 |
| ApoI | RAATTY | 6 cut(s) | 50, 473, 670, 1016, 1213, 1702 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2047, 2079 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 489 |
| AspS9I | GGNCC | 3 cut(s) | 546, 1046, 1694 |
| AsuHPI | GGTGA | 3 cut(s) | 970, 1274, 2065 |
| AsuII | TTCGAA | 1 cut(s) | 2066 |
| AvaII | GGWCC | 3 cut(s) | 546, 1046, 1694 |
| AxyI | CCTNAGG | 1 cut(s) | 1596 |
| BalI | TGGCCA | 1 cut(s) | 868 |
| BanI | GGYRCC | 2 cut(s) | 1084, 1591 |
| BbsI | GAAGAC | 1 cut(s) | 542 |
| BbvCI | CCTCAGC | 1 cut(s) | 1935 |
| BbvI | GCAGC | 6 cut(s) | 55, 286, 1066, 1497, 1559, 1857 |
| BccI | CCATC | 6 cut(s) | 191, 550, 648, 803, 911, 1337 |
| BciT130I | CCWGG | 2 cut(s) | 1059, 1524 |
| BciVI | GTATCC | 1 cut(s) | 757 |
| BclI | TGATCA | 2 cut(s) | 169, 1378 |
| BcoDI | GTCTC | 1 cut(s) | 1148 |
| BcuI | ACTAGT | 1 cut(s) | 1388 |
| BfaI | CTAG | 7 cut(s) | 213, 347, 407, 651, 735, 888, 1389 |
| BfmI | CTRYAG | 2 cut(s) | 282, 1338 |
| BfuI | GTATCC | 1 cut(s) | 757 |
| BglII | AGATCT | 1 cut(s) | 220 |
| BisI | GCNGC | 7 cut(s) | 69, 300, 1055, 1511, 1548, 1629, 1846 |
| BlsI | GCNGC | 7 cut(s) | 70, 301, 1056, 1512, 1549, 1630, 1847 |
| Bme1390I | CCNGG | 2 cut(s) | 1059, 1524 |
| Bme18I | GGWCC | 3 cut(s) | 546, 1046, 1694 |
| BmgT120I | GGNCC | 3 cut(s) | 546, 1046, 1694 |
| BmiI | GGNNCC | 4 cut(s) | 547, 1086, 1593, 1695 |
| BmrFI | CCNGG | 2 cut(s) | 1059, 1524 |
| BmsI | GCATC | 7 cut(s) | 343, 498, 603, 632, 1129, 1458, 2086 |
| BpiI | GAAGAC | 1 cut(s) | 542 |
| BpmI | CTGGAG | 1 cut(s) | 1992 |
| Bpu10I | CCTNAGC | 4 cut(s) | 1050, 1791, 1935, 2072 |
| Bpu14I | TTCGAA | 1 cut(s) | 2066 |
| BsaI | GGTCTC | 1 cut(s) | 1148 |
| BsaJI | CCNNGG | 3 cut(s) | 549, 995, 1523 |
| BsaWI | WCCGGW | 1 cut(s) | 1765 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1338, 1368 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 641, 875, 901, 1208, 1259, 1601 |
| Bse118I | RCCGGY | 1 cut(s) | 964 |
| Bse21I | CCTNAGG | 1 cut(s) | 1596 |
| BseBI | CCWGG | 2 cut(s) | 1059, 1524 |
| BseDI | CCNNGG | 3 cut(s) | 549, 995, 1523 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 641, 875, 901, 1208, 1259, 1601 |
| BseMII | CTCAG | 2 cut(s) | 1926, 2193 |
| BseXI | GCAGC | 6 cut(s) | 55, 286, 1066, 1497, 1559, 1857 |
| BsgI | GTGCAG | 2 cut(s) | 1800, 1977 |
| BshFI | GGCC | 3 cut(s) | 868, 964, 2122 |
| BshNI | GGYRCC | 2 cut(s) | 1084, 1591 |
| BsiSI | CCGG | 2 cut(s) | 965, 1766 |
| BslFI | GGGAC | 1 cut(s) | 1707 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 641, 875, 901, 1208, 1259, 1601 |
| BsmAI | GTCTC | 1 cut(s) | 1148 |
| BsmFI | GGGAC | 1 cut(s) | 1707 |
| BsnI | GGCC | 3 cut(s) | 868, 964, 2122 |
| Bso31I | GGTCTC | 1 cut(s) | 1148 |
| Bsp119I | TTCGAA | 1 cut(s) | 2066 |
| Bsp19I | CCATGG | 1 cut(s) | 995 |
| BspACI | CCGC | 4 cut(s) | 830, 1260, 1579, 1629 |
| BspANI | GGCC | 3 cut(s) | 868, 964, 2122 |
| BspCNI | CTCAG | 2 cut(s) | 1927, 2194 |
| BspLI | GGNNCC | 4 cut(s) | 547, 1086, 1593, 1695 |
| BspPI | GGATC | 2 cut(s) | 68, 1566 |
| BspT104I | TTCGAA | 1 cut(s) | 2066 |
| BspT107I | GGYRCC | 2 cut(s) | 1084, 1591 |
| BspTNI | GGTCTC | 1 cut(s) | 1148 |
| BsrFI | RCCGGY | 1 cut(s) | 964 |
| BssAI | RCCGGY | 1 cut(s) | 964 |
| BssECI | CCNNGG | 3 cut(s) | 549, 995, 1523 |
| BssNAI | GTATAC | 1 cut(s) | 1638 |
| BssT1I | CCWWGG | 2 cut(s) | 549, 995 |
| Bst1107I | GTATAC | 1 cut(s) | 1638 |
| Bst2UI | CCWGG | 2 cut(s) | 1059, 1524 |
| Bst4CI | ACNGT | 3 cut(s) | 283, 1635, 2154 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1139 |
| BstBI | TTCGAA | 1 cut(s) | 2066 |
| BstC8I | GCNNGC | 2 cut(s) | 580, 789 |
| BstDEI | CTNAG | 8 cut(s) | 393, 941, 1050, 1596, 1791, 1935, 2072, 2202 |
| BstDSI | CCRYGG | 1 cut(s) | 995 |
| BstEII | GGTNACC | 1 cut(s) | 976 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1599 |
| BstMAI | GTCTC | 1 cut(s) | 1148 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 836, 911, 1139, 1778, 1931, 1940 |
| BstNI | CCWGG | 2 cut(s) | 1059, 1524 |
| BstNSI | RCATGY | 9 cut(s) | 116, 582, 791, 865, 1251, 1448, 1799, 1871, 1982 |
| BstPI | GGTNACC | 1 cut(s) | 976 |
| BstSCI | CCNGG | 2 cut(s) | 1057, 1522 |
| BstSFI | CTRYAG | 2 cut(s) | 282, 1338 |
| BstV1I | GCAGC | 6 cut(s) | 55, 286, 1066, 1497, 1559, 1857 |
| BstV2I | GAAGAC | 1 cut(s) | 542 |
| BstX2I | RGATCY | 2 cut(s) | 220, 1558 |
| BstYI | RGATCY | 2 cut(s) | 220, 1558 |
| BstZ17I | GTATAC | 1 cut(s) | 1638 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1596 |
| BsuI | GTATCC | 1 cut(s) | 757 |
| BsuRI | GGCC | 3 cut(s) | 868, 964, 2122 |
| BtgI | CCRYGG | 1 cut(s) | 995 |
| BtsI | GCAGTG | 1 cut(s) | 1950 |
| BtsIMutI | CAGTG | 2 cut(s) | 279, 1950 |
| Cac8I | GCNNGC | 2 cut(s) | 580, 789 |
| Cfr10I | RCCGGY | 1 cut(s) | 964 |
| Cfr13I | GGNCC | 3 cut(s) | 546, 1046, 1694 |
| Csp6I | GTAC | 2 cut(s) | 698, 1976 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 422, 457, 981, 1016 |
| CviQI | GTAC | 2 cut(s) | 698, 1976 |
| DdeI | CTNAG | 8 cut(s) | 393, 941, 1050, 1596, 1791, 1935, 2072, 2202 |
| DraI | TTTAAA | 2 cut(s) | 1104, 1720 |
| DraIII | CACNNNGTG | 1 cut(s) | 437 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 544, 1073 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 544, 1073 |
| EaeI | YGGCCR | 3 cut(s) | 866, 962, 2120 |
| Eco130I | CCWWGG | 2 cut(s) | 549, 995 |
| Eco31I | GGTCTC | 1 cut(s) | 1148 |
| Eco47I | GGWCC | 3 cut(s) | 546, 1046, 1694 |
| Eco57I | CTGAAG | 3 cut(s) | 1926, 1941, 2133 |
| Eco81I | CCTNAGG | 1 cut(s) | 1596 |
| Eco91I | GGTNACC | 1 cut(s) | 976 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1599 |
| EcoO109I | RGGNCCY | 1 cut(s) | 546 |
| EcoO65I | GGTNACC | 1 cut(s) | 976 |
| EcoRI | GAATTC | 1 cut(s) | 670 |
| EcoRII | CCWGG | 2 cut(s) | 1057, 1522 |
| EcoT14I | CCWWGG | 2 cut(s) | 549, 995 |
| EcoT22I | ATGCAT | 1 cut(s) | 1144 |
| ErhI | CCWWGG | 2 cut(s) | 549, 995 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 474, 506, 1305, 1337, 2073, 2105 |
| FaqI | GGGAC | 1 cut(s) | 1707 |
| FauNDI | CATATG | 2 cut(s) | 1135, 1207 |
| FbaI | TGATCA | 2 cut(s) | 169, 1378 |
| FblI | GTMKAC | 1 cut(s) | 1637 |
| Fnu4HI | GCNGC | 7 cut(s) | 69, 300, 1055, 1511, 1548, 1629, 1846 |
| Fsp4HI | GCNGC | 7 cut(s) | 69, 300, 1055, 1511, 1548, 1629, 1846 |
| FspBI | CTAG | 7 cut(s) | 213, 347, 407, 651, 735, 888, 1389 |
| GluI | GCNGC | 7 cut(s) | 69, 300, 1055, 1511, 1548, 1629, 1846 |
| GsuI | CTGGAG | 1 cut(s) | 1992 |
| HaeIII | GGCC | 3 cut(s) | 868, 964, 2122 |
| HapII | CCGG | 2 cut(s) | 965, 1766 |
| HindIII | AAGCTT | 3 cut(s) | 454, 848, 1923 |
| HinfI | GANTC | 7 cut(s) | 240, 329, 833, 937, 1495, 1897, 1989 |
| HpaII | CCGG | 2 cut(s) | 965, 1766 |
| HphI | GGTGA | 3 cut(s) | 970, 1274, 2065 |
| Hpy166II | GTNNAC | 4 cut(s) | 110, 390, 815, 1638 |
| Hpy188I | TCNGA | 8 cut(s) | 16, 220, 413, 448, 619, 899, 1279, 1896 |
| Hpy188III | TCNNGA | 5 cut(s) | 326, 651, 1733, 1904, 1971 |
| Hpy8I | GTNNAC | 4 cut(s) | 110, 390, 815, 1638 |
| HpyAV | CCTTC | 8 cut(s) | 298, 522, 652, 738, 1327, 1595, 1645, 1901 |
| HpyCH4III | ACNGT | 3 cut(s) | 283, 1635, 2154 |
| HpyCH4IV | ACGT | 1 cut(s) | 1485 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 836, 911, 1139, 1778, 1931, 1940 |
| HpyF3I | CTNAG | 8 cut(s) | 393, 941, 1050, 1596, 1791, 1935, 2072, 2202 |
| HpySE526I | ACGT | 1 cut(s) | 1485 |
| Ksp22I | TGATCA | 2 cut(s) | 169, 1378 |
| LmnI | GCTCC | 1 cut(s) | 2035 |
| Lsp1109I | GCAGC | 6 cut(s) | 55, 286, 1066, 1497, 1559, 1857 |
| LweI | GCATC | 7 cut(s) | 343, 498, 603, 632, 1129, 1458, 2086 |
| MaeI | CTAG | 7 cut(s) | 213, 347, 407, 651, 735, 888, 1389 |
| MaeII | ACGT | 1 cut(s) | 1485 |
| MaeIII | GTNAC | 3 cut(s) | 181, 976, 2053 |
| MboII | GAAGA | 6 cut(s) | 21, 362, 547, 815, 818, 1822 |
| MflI | RGATCY | 2 cut(s) | 220, 1558 |
| MlsI | TGGCCA | 1 cut(s) | 868 |
| MluNI | TGGCCA | 1 cut(s) | 868 |
| MlyI | GAGTC | 3 cut(s) | 827, 1504, 1983 |
| MnlI | CCTC | 5 cut(s) | 130, 905, 939, 1930, 2004 |
| Mox20I | TGGCCA | 1 cut(s) | 868 |
| Mph1103I | ATGCAT | 1 cut(s) | 1144 |
| MroXI | GAANNNNTTC | 1 cut(s) | 489 |
| MscI | TGGCCA | 1 cut(s) | 868 |
| MslI | CAYNNNNRTG | 4 cut(s) | 269, 628, 1000, 1766 |
| Msp20I | TGGCCA | 1 cut(s) | 868 |
| MspA1I | CMGCKG | 2 cut(s) | 830, 2111 |
| MspI | CCGG | 2 cut(s) | 965, 1766 |
| MspR9I | CCNGG | 2 cut(s) | 1059, 1524 |
| MvaI | CCWGG | 2 cut(s) | 1059, 1524 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 836, 911, 1139, 1778, 1931, 1940 |
| NcoI | CCATGG | 1 cut(s) | 995 |
| NdeI | CATATG | 2 cut(s) | 1135, 1207 |
| NlaIV | GGNNCC | 4 cut(s) | 547, 1086, 1593, 1695 |
| NmeAIII | GCCGAG | 1 cut(s) | 382 |
| NmuCI | GTSAC | 2 cut(s) | 976, 2053 |
| NsiI | ATGCAT | 1 cut(s) | 1144 |
| NspI | RCATGY | 9 cut(s) | 116, 582, 791, 865, 1251, 1448, 1799, 1871, 1982 |
| NspV | TTCGAA | 1 cut(s) | 2066 |
| PaeI | GCATGC | 2 cut(s) | 582, 791 |
| PciI | ACATGT | 3 cut(s) | 112, 861, 1867 |
| PdmI | GAANNNNTTC | 1 cut(s) | 489 |
| PfeI | GAWTC | 4 cut(s) | 240, 329, 937, 1897 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 875 |
| PkrI | GCNGC | 7 cut(s) | 70, 301, 1056, 1512, 1549, 1630, 1847 |
| PleI | GAGTC | 3 cut(s) | 827, 1503, 1983 |
| PpsI | GAGTC | 3 cut(s) | 827, 1503, 1983 |
| PpuMI | RGGWCCY | 1 cut(s) | 546 |
| PscI | ACATGT | 3 cut(s) | 112, 861, 1867 |
| PsiI | TTATAA | 2 cut(s) | 2025, 2209 |
| Psp5II | RGGWCCY | 1 cut(s) | 546 |
| Psp6I | CCWGG | 2 cut(s) | 1057, 1522 |
| PspEI | GGTNACC | 1 cut(s) | 976 |
| PspGI | CCWGG | 2 cut(s) | 1057, 1522 |
| PspN4I | GGNNCC | 4 cut(s) | 547, 1086, 1593, 1695 |
| PspPI | GGNCC | 3 cut(s) | 546, 1046, 1694 |
| PspPPI | RGGWCCY | 1 cut(s) | 546 |
| PsuI | RGATCY | 2 cut(s) | 220, 1558 |
| PvuII | CAGCTG | 1 cut(s) | 2111 |
| RsaI | GTAC | 2 cut(s) | 699, 1977 |
| RsaNI | GTAC | 2 cut(s) | 698, 1976 |
| RseI | CAYNNNNRTG | 4 cut(s) | 269, 628, 1000, 1766 |
| SatI | GCNGC | 7 cut(s) | 69, 300, 1055, 1511, 1548, 1629, 1846 |
| Sau96I | GGNCC | 3 cut(s) | 546, 1046, 1694 |
| SchI | GAGTC | 3 cut(s) | 827, 1504, 1983 |
| ScrFI | CCNGG | 2 cut(s) | 1059, 1524 |
| SfaNI | GCATC | 7 cut(s) | 343, 498, 603, 632, 1129, 1458, 2086 |
| SfcI | CTRYAG | 2 cut(s) | 282, 1338 |
| SfuI | TTCGAA | 1 cut(s) | 2066 |
| SinI | GGWCC | 3 cut(s) | 546, 1046, 1694 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 269, 628, 1000, 1766 |
| SpeI | ACTAGT | 1 cut(s) | 1388 |
| SphI | GCATGC | 2 cut(s) | 582, 791 |
| SsiI | CCGC | 4 cut(s) | 830, 1260, 1579, 1629 |
| SspI | AATATT | 2 cut(s) | 1413, 2021 |
| SspMI | CTAG | 7 cut(s) | 213, 347, 407, 651, 735, 888, 1389 |
| StyD4I | CCNGG | 2 cut(s) | 1057, 1522 |
| StyI | CCWWGG | 2 cut(s) | 549, 995 |
| TaaI | ACNGT | 3 cut(s) | 283, 1635, 2154 |
| TaiI | ACGT | 1 cut(s) | 1488 |
| TaqI | TCGA | 1 cut(s) | 2066 |
| TatI | WGTACW | 1 cut(s) | 1975 |
| TauI | GCSGC | 1 cut(s) | 1631 |
| TfiI | GAWTC | 4 cut(s) | 240, 329, 937, 1897 |
| TscAI | CASTG | 2 cut(s) | 286, 1950 |
| TseFI | GTSAC | 2 cut(s) | 976, 2053 |
| TseI | GCWGC | 6 cut(s) | 68, 299, 1054, 1510, 1547, 1845 |
| Tsp45I | GTSAC | 2 cut(s) | 976, 2053 |
| TspDTI | ATGAA | 6 cut(s) | 33, 324, 515, 540, 831, 1022 |
| TspRI | CASTG | 2 cut(s) | 286, 1950 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 875 |
| VpaK11BI | GGWCC | 3 cut(s) | 546, 1046, 1694 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1599 |
| XapI | RAATTY | 6 cut(s) | 50, 473, 670, 1016, 1213, 1702 |
| XbaI | TCTAGA | 1 cut(s) | 650 |
| XceI | RCATGY | 9 cut(s) | 116, 582, 791, 865, 1251, 1448, 1799, 1871, 1982 |
| XmiI | GTMKAC | 1 cut(s) | 1637 |
| XmnI | GAANNNNTTC | 1 cut(s) | 489 |
| XspI | CTAG | 7 cut(s) | 213, 347, 407, 651, 735, 888, 1389 |
| Zsp2I | ATGCAT | 1 cut(s) | 1144 |