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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1364 |
| Acc65I | GGTACC | 1 cut(s) | 197 |
| AccB1I | GGYRCC | 1 cut(s) | 197 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 110 |
| AccI | GTMKAC | 2 cut(s) | 1383, 1452 |
| AclI | AACGTT | 1 cut(s) | 1064 |
| AclWI | GGATC | 1 cut(s) | 1329 |
| AcsI | RAATTY | 2 cut(s) | 894, 1536 |
| AcuI | CTGAAG | 1 cut(s) | 1035 |
| AcyI | GRCGYC | 1 cut(s) | 1443 |
| AfaI | GTAC | 5 cut(s) | 199, 746, 761, 1435, 1665 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 12, 110, 293, 756, 944, 1245 |
| AflIII | ACRYGT | 1 cut(s) | 1525 |
| AjnI | CCWGG | 1 cut(s) | 292 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 1116, 1148, 1189, 1221 |
| AleI | CACNNNNGTG | 1 cut(s) | 996 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1049, 1081 |
| AluBI | AGCT | 3 cut(s) | 59, 842, 1520 |
| AluI | AGCT | 3 cut(s) | 59, 842, 1520 |
| Alw26I | GTCTC | 2 cut(s) | 28, 427 |
| AlwI | GGATC | 1 cut(s) | 1329 |
| Ama87I | CYCGRG | 1 cut(s) | 215 |
| AoxI | GGCC | 2 cut(s) | 3, 1177 |
| ApeKI | GCWGC | 3 cut(s) | 59, 62, 180 |
| ApoI | RAATTY | 2 cut(s) | 894, 1536 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 770 |
| Asp718I | GGTACC | 1 cut(s) | 197 |
| AspA2I | CCTAGG | 1 cut(s) | 1070 |
| AspLEI | GCGC | 1 cut(s) | 950 |
| AspS9I | GGNCC | 3 cut(s) | 296, 722, 1178 |
| AsuC2I | CCSGG | 4 cut(s) | 216, 217, 494, 1247 |
| AsuHPI | GGTGA | 3 cut(s) | 93, 487, 1486 |
| AsuII | TTCGAA | 1 cut(s) | 787 |
| AsuNHI | GCTAGC | 1 cut(s) | 985 |
| AvaI | CYCGRG | 1 cut(s) | 215 |
| AvaII | GGWCC | 2 cut(s) | 296, 722 |
| AvrII | CCTAGG | 1 cut(s) | 1070 |
| AxyI | CCTNAGG | 1 cut(s) | 804 |
| BaeGI | GKGCMC | 1 cut(s) | 1592 |
| BanI | GGYRCC | 1 cut(s) | 197 |
| BbsI | GAAGAC | 1 cut(s) | 227 |
| BbvI | GCAGC | 3 cut(s) | 46, 49, 167 |
| BccI | CCATC | 8 cut(s) | 224, 421, 732, 1162, 1565, 1684, 1697, 1727 |
| BceAI | ACGGC | 1 cut(s) | 6 |
| BciT130I | CCWGG | 1 cut(s) | 294 |
| BciVI | GTATCC | 3 cut(s) | 875, 1265, 1395 |
| BcnI | CCSGG | 4 cut(s) | 216, 217, 494, 1247 |
| BcoDI | GTCTC | 2 cut(s) | 28, 427 |
| BfaI | CTAG | 6 cut(s) | 986, 1071, 1173, 1437, 1460, 1716 |
| BfmI | CTRYAG | 1 cut(s) | 843 |
| BfuI | GTATCC | 3 cut(s) | 875, 1265, 1395 |
| BisI | GCNGC | 3 cut(s) | 60, 63, 181 |
| BlnI | CCTAGG | 1 cut(s) | 1070 |
| BlpI | GCTNAGC | 1 cut(s) | 1023 |
| BlsI | GCNGC | 3 cut(s) | 61, 64, 182 |
| BmcAI | AGTACT | 1 cut(s) | 1435 |
| Bme1390I | CCNGG | 5 cut(s) | 216, 217, 294, 494, 1247 |
| Bme18I | GGWCC | 2 cut(s) | 296, 722 |
| BmeT110I | CYCGRG | 1 cut(s) | 215 |
| BmgT120I | GGNCC | 3 cut(s) | 296, 722, 1178 |
| BmiI | GGNNCC | 2 cut(s) | 199, 387 |
| BmrFI | CCNGG | 5 cut(s) | 216, 217, 294, 494, 1247 |
| BmsI | GCATC | 7 cut(s) | 25, 150, 175, 868, 1009, 1285, 1552 |
| BmtI | GCTAGC | 1 cut(s) | 989 |
| BpiI | GAAGAC | 1 cut(s) | 227 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1023 |
| Bpu14I | TTCGAA | 1 cut(s) | 787 |
| BpuMI | CCSGG | 4 cut(s) | 216, 217, 494, 1247 |
| Bsa29I | ATCGAT | 1 cut(s) | 1560 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1707 |
| BsaHI | GRCGYC | 1 cut(s) | 1443 |
| BsaI | GGTCTC | 1 cut(s) | 427 |
| BsaJI | CCNNGG | 6 cut(s) | 215, 954, 1070, 1317, 1331, 1606 |
| BsaWI | WCCGGW | 1 cut(s) | 724 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 105, 135, 1049, 1079 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 12, 110, 293, 756, 944, 1245 |
| Bse118I | RCCGGY | 1 cut(s) | 523 |
| Bse1I | ACTGG | 3 cut(s) | 447, 1574, 1784 |
| Bse21I | CCTNAGG | 1 cut(s) | 804 |
| Bse3DI | GCAATG | 1 cut(s) | 1305 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1707 |
| BseBI | CCWGG | 1 cut(s) | 294 |
| BseCI | ATCGAT | 1 cut(s) | 1560 |
| BseDI | CCNNGG | 6 cut(s) | 215, 954, 1070, 1317, 1331, 1606 |
| BseGI | GGATG | 5 cut(s) | 40, 235, 747, 1101, 1173 |
| BseJI | GATNNNNATC | 1 cut(s) | 1707 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 12, 110, 293, 756, 944, 1245 |
| BseMI | GCAATG | 1 cut(s) | 1305 |
| BseMII | CTCAG | 3 cut(s) | 795, 810, 1037 |
| BseNI | ACTGG | 3 cut(s) | 447, 1574, 1784 |
| BseRI | GAGGAG | 2 cut(s) | 391, 1640 |
| BseSI | GKGCMC | 1 cut(s) | 1592 |
| BseXI | GCAGC | 3 cut(s) | 46, 49, 167 |
| BshFI | GGCC | 2 cut(s) | 5, 1179 |
| BshNI | GGYRCC | 1 cut(s) | 197 |
| BshVI | ATCGAT | 1 cut(s) | 1560 |
| BsiHKCI | CYCGRG | 1 cut(s) | 215 |
| BsiSI | CCGG | 5 cut(s) | 216, 494, 524, 725, 1246 |
| BslFI | GGGAC | 1 cut(s) | 1262 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 12, 110, 293, 756, 944, 1245 |
| BsmAI | GTCTC | 2 cut(s) | 28, 427 |
| BsmFI | GGGAC | 1 cut(s) | 1262 |
| BsnI | GGCC | 2 cut(s) | 5, 1179 |
| Bso31I | GGTCTC | 1 cut(s) | 427 |
| BsoBI | CYCGRG | 1 cut(s) | 215 |
| Bsp119I | TTCGAA | 1 cut(s) | 787 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1592 |
| Bsp143I | GATC | 3 cut(s) | 506, 859, 1321 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1023 |
| Bsp19I | CCATGG | 2 cut(s) | 954, 1606 |
| BspANI | GGCC | 2 cut(s) | 5, 1179 |
| BspCNI | CTCAG | 3 cut(s) | 796, 811, 1036 |
| BspDI | ATCGAT | 1 cut(s) | 1560 |
| BspHI | TCATGA | 1 cut(s) | 1774 |
| BspLI | GGNNCC | 2 cut(s) | 199, 387 |
| BspOI | GCTAGC | 1 cut(s) | 989 |
| BspPI | GGATC | 1 cut(s) | 1329 |
| BspT104I | TTCGAA | 1 cut(s) | 787 |
| BspT107I | GGYRCC | 1 cut(s) | 197 |
| BspTNI | GGTCTC | 1 cut(s) | 427 |
| BsrDI | GCAATG | 1 cut(s) | 1305 |
| BsrFI | RCCGGY | 1 cut(s) | 523 |
| BsrI | ACTGG | 3 cut(s) | 447, 1574, 1784 |
| BssAI | RCCGGY | 1 cut(s) | 523 |
| BssECI | CCNNGG | 6 cut(s) | 215, 954, 1070, 1317, 1331, 1606 |
| BssMI | GATC | 3 cut(s) | 506, 859, 1321 |
| BssNI | GRCGYC | 1 cut(s) | 1443 |
| BssT1I | CCWWGG | 5 cut(s) | 954, 1070, 1317, 1331, 1606 |
| Bst2UI | CCWGG | 1 cut(s) | 294 |
| Bst4CI | ACNGT | 7 cut(s) | 385, 475, 631, 691, 889, 930, 1253 |
| Bst6I | CTCTTC | 2 cut(s) | 339, 1617 |
| BstACI | GRCGYC | 1 cut(s) | 1443 |
| BstBI | TTCGAA | 1 cut(s) | 787 |
| BstC8I | GCNNGC | 4 cut(s) | 53, 57, 612, 987 |
| BstDEI | CTNAG | 3 cut(s) | 804, 819, 1023 |
| BstDSI | CCRYGG | 2 cut(s) | 954, 1606 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 10, 754 |
| BstF5I | GGATG | 5 cut(s) | 40, 235, 747, 1101, 1173 |
| BstHHI | GCGC | 1 cut(s) | 950 |
| BstKTI | GATC | 3 cut(s) | 509, 862, 1324 |
| BstMAI | GTCTC | 2 cut(s) | 28, 427 |
| BstMBI | GATC | 3 cut(s) | 506, 859, 1321 |
| BstNI | CCWGG | 1 cut(s) | 294 |
| BstNSI | RCATGY | 1 cut(s) | 1499 |
| BstSCI | CCNGG | 5 cut(s) | 214, 215, 292, 492, 1245 |
| BstSFI | CTRYAG | 1 cut(s) | 843 |
| BstSLI | GKGCMC | 1 cut(s) | 1592 |
| BstV1I | GCAGC | 3 cut(s) | 46, 49, 167 |
| BstV2I | GAAGAC | 1 cut(s) | 227 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1220 |
| Bsu15I | ATCGAT | 1 cut(s) | 1560 |
| Bsu36I | CCTNAGG | 1 cut(s) | 804 |
| BsuI | GTATCC | 3 cut(s) | 875, 1265, 1395 |
| BsuRI | GGCC | 2 cut(s) | 5, 1179 |
| BsuTUI | ATCGAT | 1 cut(s) | 1560 |
| BtgI | CCRYGG | 2 cut(s) | 954, 1606 |
| BtgZI | GCGATG | 3 cut(s) | 2, 198, 1752 |
| BtsCI | GGATG | 5 cut(s) | 40, 235, 747, 1101, 1173 |
| BtsIMutI | CAGTG | 2 cut(s) | 885, 935 |
| Cac8I | GCNNGC | 4 cut(s) | 53, 57, 612, 987 |
| CciI | TCATGA | 1 cut(s) | 1774 |
| CfoI | GCGC | 1 cut(s) | 950 |
| Cfr10I | RCCGGY | 1 cut(s) | 523 |
| Cfr13I | GGNCC | 3 cut(s) | 296, 722, 1178 |
| Cfr9I | CCCGGG | 1 cut(s) | 215 |
| ClaI | ATCGAT | 1 cut(s) | 1560 |
| CseI | GACGC | 2 cut(s) | 991, 1432 |
| CsiI | ACCWGGT | 1 cut(s) | 292 |
| Csp6I | GTAC | 5 cut(s) | 198, 745, 760, 1434, 1664 |
| CviQI | GTAC | 5 cut(s) | 198, 745, 760, 1434, 1664 |
| DdeI | CTNAG | 3 cut(s) | 804, 819, 1023 |
| DpnI | GATC | 3 cut(s) | 508, 861, 1323 |
| DpnII | GATC | 3 cut(s) | 506, 859, 1321 |
| Eam1104I | CTCTTC | 2 cut(s) | 339, 1617 |
| EarI | CTCTTC | 2 cut(s) | 339, 1617 |
| Eco130I | CCWWGG | 5 cut(s) | 954, 1070, 1317, 1331, 1606 |
| Eco31I | GGTCTC | 1 cut(s) | 427 |
| Eco47I | GGWCC | 2 cut(s) | 296, 722 |
| Eco57I | CTGAAG | 1 cut(s) | 1035 |
| Eco81I | CCTNAGG | 1 cut(s) | 804 |
| Eco88I | CYCGRG | 1 cut(s) | 215 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 10, 754 |
| EcoRI | GAATTC | 1 cut(s) | 894 |
| EcoRII | CCWGG | 1 cut(s) | 292 |
| EcoT14I | CCWWGG | 5 cut(s) | 954, 1070, 1317, 1331, 1606 |
| ErhI | CCWWGG | 5 cut(s) | 954, 1070, 1317, 1331, 1606 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1189, 1221 |
| FaqI | GGGAC | 1 cut(s) | 1262 |
| FauNDI | CATATG | 2 cut(s) | 1044, 1725 |
| FblI | GTMKAC | 2 cut(s) | 1383, 1452 |
| Fnu4HI | GCNGC | 3 cut(s) | 60, 63, 181 |
| FokI | GGATG | 5 cut(s) | 27, 242, 754, 1108, 1180 |
| Fsp4HI | GCNGC | 3 cut(s) | 60, 63, 181 |
| FspBI | CTAG | 6 cut(s) | 986, 1071, 1173, 1437, 1460, 1716 |
| GlaI | GCGC | 1 cut(s) | 949 |
| GluI | GCNGC | 3 cut(s) | 60, 63, 181 |
| HaeIII | GGCC | 2 cut(s) | 5, 1179 |
| HapII | CCGG | 5 cut(s) | 216, 494, 524, 725, 1246 |
| HgaI | GACGC | 2 cut(s) | 991, 1432 |
| HhaI | GCGC | 1 cut(s) | 950 |
| Hin1I | GRCGYC | 1 cut(s) | 1443 |
| Hin6I | GCGC | 1 cut(s) | 948 |
| HinP1I | GCGC | 1 cut(s) | 948 |
| HincII | GTYRAC | 4 cut(s) | 981, 1062, 1228, 1756 |
| HindII | GTYRAC | 4 cut(s) | 981, 1062, 1228, 1756 |
| HinfI | GANTC | 4 cut(s) | 267, 415, 478, 679 |
| HpaI | GTTAAC | 1 cut(s) | 1062 |
| HpaII | CCGG | 5 cut(s) | 216, 494, 524, 725, 1246 |
| HphI | GGTGA | 3 cut(s) | 93, 487, 1486 |
| Hpy166II | GTNNAC | 7 cut(s) | 981, 1062, 1228, 1384, 1453, 1467, 1756 |
| Hpy188I | TCNGA | 5 cut(s) | 272, 859, 1153, 1259, 1269 |
| Hpy8I | GTNNAC | 7 cut(s) | 981, 1062, 1228, 1384, 1453, 1467, 1756 |
| Hpy99I | CGWCG | 1 cut(s) | 674 |
| HpyAV | CCTTC | 4 cut(s) | 108, 669, 878, 1736 |
| HpyCH4III | ACNGT | 7 cut(s) | 385, 475, 631, 691, 889, 930, 1253 |
| HpyCH4IV | ACGT | 3 cut(s) | 672, 1064, 1527 |
| HpyF3I | CTNAG | 3 cut(s) | 804, 819, 1023 |
| HpySE526I | ACGT | 3 cut(s) | 672, 1064, 1527 |
| Hsp92I | GRCGYC | 1 cut(s) | 1443 |
| HspAI | GCGC | 1 cut(s) | 948 |
| KpnI | GGTACC | 1 cut(s) | 201 |
| KspAI | GTTAAC | 1 cut(s) | 1062 |
| Kzo9I | GATC | 3 cut(s) | 506, 859, 1321 |
| LmnI | GCTCC | 1 cut(s) | 1039 |
| Lsp1109I | GCAGC | 3 cut(s) | 46, 49, 167 |
| LweI | GCATC | 7 cut(s) | 25, 150, 175, 868, 1009, 1285, 1552 |
| MabI | ACCWGGT | 1 cut(s) | 292 |
| MaeI | CTAG | 6 cut(s) | 986, 1071, 1173, 1437, 1460, 1716 |
| MaeII | ACGT | 3 cut(s) | 672, 1064, 1527 |
| MalI | GATC | 3 cut(s) | 508, 861, 1323 |
| MboI | GATC | 3 cut(s) | 506, 859, 1321 |
| MhlI | GDGCHC | 1 cut(s) | 1592 |
| MmeI | TCCRAC | 1 cut(s) | 308 |
| MroXI | GAANNNNTTC | 1 cut(s) | 770 |
| MseI | TTAA | 7 cut(s) | 107, 437, 453, 600, 686, 1061, 1530 |
| MslI | CAYNNNNRTG | 5 cut(s) | 767, 996, 1218, 1500, 1724 |
| MspI | CCGG | 5 cut(s) | 216, 494, 524, 725, 1246 |
| MspR9I | CCNGG | 5 cut(s) | 216, 217, 294, 494, 1247 |
| MvaI | CCWGG | 1 cut(s) | 294 |
| NciI | CCSGG | 4 cut(s) | 216, 217, 494, 1247 |
| NcoI | CCATGG | 2 cut(s) | 954, 1606 |
| NdeI | CATATG | 2 cut(s) | 1044, 1725 |
| NdeII | GATC | 3 cut(s) | 506, 859, 1321 |
| NheI | GCTAGC | 1 cut(s) | 985 |
| NlaIV | GGNNCC | 2 cut(s) | 199, 387 |
| NmeAIII | GCCGAG | 1 cut(s) | 57 |
| NspI | RCATGY | 1 cut(s) | 1499 |
| NspV | TTCGAA | 1 cut(s) | 787 |
| OliI | CACNNNNGTG | 1 cut(s) | 996 |
| PagI | TCATGA | 1 cut(s) | 1774 |
| PdmI | GAANNNNTTC | 1 cut(s) | 770 |
| PfeI | GAWTC | 4 cut(s) | 267, 415, 478, 679 |
| PflFI | GACNNNGTC | 1 cut(s) | 254 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 110 |
| PkrI | GCNGC | 3 cut(s) | 61, 64, 182 |
| PsiI | TTATAA | 1 cut(s) | 1364 |
| Psp1406I | AACGTT | 1 cut(s) | 1064 |
| Psp6I | CCWGG | 1 cut(s) | 292 |
| PspGI | CCWGG | 1 cut(s) | 292 |
| PspN4I | GGNNCC | 2 cut(s) | 199, 387 |
| PspPI | GGNCC | 3 cut(s) | 296, 722, 1178 |
| PsyI | GACNNNGTC | 1 cut(s) | 254 |
| RsaI | GTAC | 5 cut(s) | 199, 746, 761, 1435, 1665 |
| RsaNI | GTAC | 5 cut(s) | 198, 745, 760, 1434, 1664 |
| RseI | CAYNNNNRTG | 5 cut(s) | 767, 996, 1218, 1500, 1724 |
| SaqAI | TTAA | 7 cut(s) | 107, 437, 453, 600, 686, 1061, 1530 |
| SatI | GCNGC | 3 cut(s) | 60, 63, 181 |
| Sau3AI | GATC | 3 cut(s) | 506, 859, 1321 |
| Sau96I | GGNCC | 3 cut(s) | 296, 722, 1178 |
| ScaI | AGTACT | 1 cut(s) | 1435 |
| ScrFI | CCNGG | 5 cut(s) | 216, 217, 294, 494, 1247 |
| SduI | GDGCHC | 1 cut(s) | 1592 |
| SexAI | ACCWGGT | 1 cut(s) | 292 |
| SfaNI | GCATC | 7 cut(s) | 25, 150, 175, 868, 1009, 1285, 1552 |
| SfcI | CTRYAG | 1 cut(s) | 843 |
| SfuI | TTCGAA | 1 cut(s) | 787 |
| SinI | GGWCC | 2 cut(s) | 296, 722 |
| SmaI | CCCGGG | 1 cut(s) | 217 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 767, 996, 1218, 1500, 1724 |
| SspMI | CTAG | 6 cut(s) | 986, 1071, 1173, 1437, 1460, 1716 |
| StyD4I | CCNGG | 5 cut(s) | 214, 215, 292, 492, 1245 |
| StyI | CCWWGG | 5 cut(s) | 954, 1070, 1317, 1331, 1606 |
| TaaI | ACNGT | 7 cut(s) | 385, 475, 631, 691, 889, 930, 1253 |
| TaiI | ACGT | 3 cut(s) | 675, 1067, 1530 |
| TaqI | TCGA | 8 cut(s) | 258, 425, 669, 736, 787, 793, 1560, 1683 |
| TatI | WGTACW | 1 cut(s) | 1433 |
| TfiI | GAWTC | 4 cut(s) | 267, 415, 478, 679 |
| Tru1I | TTAA | 7 cut(s) | 107, 437, 453, 600, 686, 1061, 1530 |
| Tru9I | TTAA | 7 cut(s) | 107, 437, 453, 600, 686, 1061, 1530 |
| TscAI | CASTG | 2 cut(s) | 892, 935 |
| TseI | GCWGC | 3 cut(s) | 59, 62, 180 |
| TspDTI | ATGAA | 9 cut(s) | 671, 683, 779, 1024, 1197, 1203, 1635, 1755, 1776 |
| TspMI | CCCGGG | 1 cut(s) | 215 |
| TspRI | CASTG | 2 cut(s) | 892, 935 |
| Tth111I | GACNNNGTC | 1 cut(s) | 254 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 110 |
| VpaK11BI | GGWCC | 2 cut(s) | 296, 722 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 10, 754 |
| XapI | RAATTY | 2 cut(s) | 894, 1536 |
| XceI | RCATGY | 1 cut(s) | 1499 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 316 |
| XmaI | CCCGGG | 1 cut(s) | 215 |
| XmaJI | CCTAGG | 1 cut(s) | 1070 |
| XmiI | GTMKAC | 2 cut(s) | 1383, 1452 |
| XmnI | GAANNNNTTC | 1 cut(s) | 770 |
| XspI | CTAG | 6 cut(s) | 986, 1071, 1173, 1437, 1460, 1716 |
| ZrmI | AGTACT | 1 cut(s) | 1435 |