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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 766 |
| AccB1I | GGYRCC | 1 cut(s) | 766 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 944, 1277 |
| AccI | GTMKAC | 2 cut(s) | 396, 924 |
| AccIII | TCCGGA | 1 cut(s) | 1389 |
| AciI | CCGC | 1 cut(s) | 889 |
| AclWI | GGATC | 1 cut(s) | 177 |
| AcoI | YGGCCR | 2 cut(s) | 935, 1031 |
| AcsI | RAATTY | 2 cut(s) | 62, 941 |
| AcuI | CTGAAG | 2 cut(s) | 288, 591 |
| AfaI | GTAC | 5 cut(s) | 446, 457, 768, 788, 1148 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 258, 716, 912, 944, 1277 |
| AflII | CTTAAG | 1 cut(s) | 68 |
| AflIII | ACRYGT | 2 cut(s) | 540, 713 |
| AgsI | TTSAA | 3 cut(s) | 153, 824, 1440 |
| AjnI | CCWGG | 2 cut(s) | 448, 586 |
| AluBI | AGCT | 5 cut(s) | 49, 272, 440, 1005, 1274 |
| AluI | AGCT | 5 cut(s) | 49, 272, 440, 1005, 1274 |
| AlwI | GGATC | 1 cut(s) | 177 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1468 |
| Aor13HI | TCCGGA | 1 cut(s) | 1389 |
| AoxI | GGCC | 3 cut(s) | 603, 935, 1031 |
| ApeKI | GCWGC | 1 cut(s) | 1153 |
| ApoI | RAATTY | 2 cut(s) | 62, 941 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 198, 230, 735, 767 |
| AseI | ATTAAT | 2 cut(s) | 993, 1260 |
| Asp718I | GGTACC | 1 cut(s) | 766 |
| AspA2I | CCTAGG | 1 cut(s) | 956 |
| AspLEI | GCGC | 1 cut(s) | 816 |
| AspS9I | GGNCC | 1 cut(s) | 1115 |
| AsuC2I | CCSGG | 2 cut(s) | 771, 1128 |
| AsuHPI | GGTGA | 1 cut(s) | 359 |
| AvaII | GGWCC | 1 cut(s) | 1115 |
| AvrII | CCTAGG | 1 cut(s) | 956 |
| BalI | TGGCCA | 2 cut(s) | 937, 1033 |
| BanI | GGYRCC | 1 cut(s) | 766 |
| BbvI | GCAGC | 1 cut(s) | 1165 |
| BccI | CCATC | 7 cut(s) | 134, 293, 422, 1181, 1190, 1389, 1415 |
| BciT130I | CCWGG | 2 cut(s) | 450, 588 |
| BclI | TGATCA | 1 cut(s) | 546 |
| BcnI | CCSGG | 2 cut(s) | 771, 1128 |
| BfaI | CTAG | 4 cut(s) | 50, 90, 234, 957 |
| BfmI | CTRYAG | 2 cut(s) | 276, 1000 |
| BfrI | CTTAAG | 1 cut(s) | 68 |
| BisI | GCNGC | 2 cut(s) | 890, 1154 |
| BlnI | CCTAGG | 1 cut(s) | 956 |
| BlsI | GCNGC | 2 cut(s) | 891, 1155 |
| Bme1390I | CCNGG | 4 cut(s) | 450, 588, 771, 1128 |
| Bme18I | GGWCC | 1 cut(s) | 1115 |
| BmgT120I | GGNCC | 1 cut(s) | 1115 |
| BmiI | GGNNCC | 3 cut(s) | 768, 962, 1117 |
| BmrFI | CCNGG | 4 cut(s) | 450, 588, 771, 1128 |
| BmsI | GCATC | 6 cut(s) | 6, 358, 892, 901, 1083, 1198 |
| BpuMI | CCSGG | 2 cut(s) | 771, 1128 |
| BsaAI | YACGTR | 1 cut(s) | 1050 |
| BsaBI | GATNNNNATC | 1 cut(s) | 551 |
| BsaJI | CCNNGG | 4 cut(s) | 448, 770, 956, 1064 |
| BsaWI | WCCGGW | 1 cut(s) | 1389 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 675, 705, 713, 743 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 258, 716, 912, 944, 1277 |
| Bse1I | ACTGG | 1 cut(s) | 1034 |
| Bse3DI | GCAATG | 1 cut(s) | 246 |
| Bse8I | GATNNNNATC | 1 cut(s) | 551 |
| BseAI | TCCGGA | 1 cut(s) | 1389 |
| BseBI | CCWGG | 2 cut(s) | 450, 588 |
| BseDI | CCNNGG | 4 cut(s) | 448, 770, 956, 1064 |
| BseGI | GGATG | 8 cut(s) | 21, 358, 385, 433, 883, 892, 1192, 1410 |
| BseJI | GATNNNNATC | 1 cut(s) | 551 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 258, 716, 912, 944, 1277 |
| BseMI | GCAATG | 1 cut(s) | 246 |
| BseNI | ACTGG | 1 cut(s) | 1034 |
| BseRI | GAGGAG | 1 cut(s) | 696 |
| BseXI | GCAGC | 1 cut(s) | 1165 |
| BseYI | CCCAGC | 2 cut(s) | 371, 1270 |
| BshFI | GGCC | 3 cut(s) | 605, 937, 1033 |
| BshNI | GGYRCC | 1 cut(s) | 766 |
| BsiSI | CCGG | 4 cut(s) | 770, 896, 1128, 1390 |
| BslFI | GGGAC | 1 cut(s) | 157 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 258, 716, 912, 944, 1277 |
| BsmFI | GGGAC | 1 cut(s) | 157 |
| BsmI | GAATGC | 2 cut(s) | 91, 253 |
| BsnI | GGCC | 3 cut(s) | 605, 937, 1033 |
| Bsp13I | TCCGGA | 1 cut(s) | 1389 |
| Bsp143I | GATC | 5 cut(s) | 32, 169, 325, 546, 552 |
| Bsp19I | CCATGG | 1 cut(s) | 1064 |
| BspACI | CCGC | 1 cut(s) | 889 |
| BspANI | GGCC | 3 cut(s) | 605, 937, 1033 |
| BspEI | TCCGGA | 1 cut(s) | 1389 |
| BspHI | TCATGA | 2 cut(s) | 549, 1089 |
| BspLI | GGNNCC | 3 cut(s) | 768, 962, 1117 |
| BspPI | GGATC | 1 cut(s) | 177 |
| BspT107I | GGYRCC | 1 cut(s) | 766 |
| BspTI | CTTAAG | 1 cut(s) | 68 |
| BsrDI | GCAATG | 1 cut(s) | 246 |
| BsrI | ACTGG | 1 cut(s) | 1034 |
| BssECI | CCNNGG | 4 cut(s) | 448, 770, 956, 1064 |
| BssMI | GATC | 5 cut(s) | 32, 169, 325, 546, 552 |
| BssT1I | CCWWGG | 2 cut(s) | 956, 1064 |
| Bst2UI | CCWGG | 2 cut(s) | 450, 588 |
| Bst4CI | ACNGT | 4 cut(s) | 277, 807, 1104, 1436 |
| Bst6I | CTCTTC | 1 cut(s) | 285 |
| BstAFI | CTTAAG | 1 cut(s) | 68 |
| BstBAI | YACGTR | 1 cut(s) | 1050 |
| BstC8I | GCNNGC | 2 cut(s) | 21, 858 |
| BstDEI | CTNAG | 2 cut(s) | 965, 1302 |
| BstDSI | CCRYGG | 1 cut(s) | 1064 |
| BstEII | GGTNACC | 1 cut(s) | 1398 |
| BstF5I | GGATG | 8 cut(s) | 21, 358, 385, 433, 883, 892, 1192, 1410 |
| BstHHI | GCGC | 1 cut(s) | 816 |
| BstKTI | GATC | 5 cut(s) | 35, 172, 328, 549, 555 |
| BstMBI | GATC | 5 cut(s) | 32, 169, 325, 546, 552 |
| BstNI | CCWGG | 2 cut(s) | 450, 588 |
| BstNSI | RCATGY | 9 cut(s) | 23, 531, 544, 653, 657, 692, 717, 820, 860 |
| BstPI | GGTNACC | 1 cut(s) | 1398 |
| BstSCI | CCNGG | 4 cut(s) | 448, 586, 769, 1126 |
| BstSFI | CTRYAG | 2 cut(s) | 276, 1000 |
| BstV1I | GCAGC | 1 cut(s) | 1165 |
| BsuRI | GGCC | 3 cut(s) | 605, 937, 1033 |
| BtgI | CCRYGG | 1 cut(s) | 1064 |
| BtsCI | GGATG | 8 cut(s) | 21, 358, 385, 433, 883, 892, 1192, 1410 |
| BtsI | GCAGTG | 1 cut(s) | 1300 |
| BtsIMutI | CAGTG | 2 cut(s) | 1100, 1300 |
| Cac8I | GCNNGC | 2 cut(s) | 21, 858 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1468 |
| CciI | TCATGA | 2 cut(s) | 549, 1089 |
| CfoI | GCGC | 1 cut(s) | 816 |
| Cfr13I | GGNCC | 1 cut(s) | 1115 |
| CsiI | ACCWGGT | 1 cut(s) | 586 |
| Csp6I | GTAC | 5 cut(s) | 445, 456, 767, 787, 1147 |
| CviQI | GTAC | 5 cut(s) | 445, 456, 767, 787, 1147 |
| DdeI | CTNAG | 2 cut(s) | 965, 1302 |
| DpnI | GATC | 5 cut(s) | 34, 171, 327, 548, 554 |
| DpnII | GATC | 5 cut(s) | 32, 169, 325, 546, 552 |
| DraI | TTTAAA | 2 cut(s) | 1173, 1231 |
| EaeI | YGGCCR | 2 cut(s) | 935, 1031 |
| Eam1104I | CTCTTC | 1 cut(s) | 285 |
| EarI | CTCTTC | 1 cut(s) | 285 |
| Eco130I | CCWWGG | 2 cut(s) | 956, 1064 |
| Eco47I | GGWCC | 1 cut(s) | 1115 |
| Eco57I | CTGAAG | 2 cut(s) | 288, 591 |
| Eco91I | GGTNACC | 1 cut(s) | 1398 |
| EcoO65I | GGTNACC | 1 cut(s) | 1398 |
| EcoRII | CCWGG | 2 cut(s) | 448, 586 |
| EcoT14I | CCWWGG | 2 cut(s) | 956, 1064 |
| EcoT22I | ATGCAT | 4 cut(s) | 21, 655, 1076, 1213 |
| ErhI | CCWWGG | 2 cut(s) | 956, 1064 |
| FaqI | GGGAC | 1 cut(s) | 157 |
| FbaI | TGATCA | 1 cut(s) | 546 |
| FblI | GTMKAC | 2 cut(s) | 396, 924 |
| Fnu4HI | GCNGC | 2 cut(s) | 890, 1154 |
| FokI | GGATG | 8 cut(s) | 28, 365, 392, 440, 879, 890, 1199, 1397 |
| Fsp4HI | GCNGC | 2 cut(s) | 890, 1154 |
| FspBI | CTAG | 4 cut(s) | 50, 90, 234, 957 |
| GlaI | GCGC | 1 cut(s) | 815 |
| GluI | GCNGC | 2 cut(s) | 890, 1154 |
| GsaI | CCCAGC | 2 cut(s) | 375, 1274 |
| HaeIII | GGCC | 3 cut(s) | 605, 937, 1033 |
| HapII | CCGG | 4 cut(s) | 770, 896, 1128, 1390 |
| HhaI | GCGC | 1 cut(s) | 816 |
| Hin6I | GCGC | 1 cut(s) | 814 |
| HinP1I | GCGC | 1 cut(s) | 814 |
| HindIII | AAGCTT | 1 cut(s) | 438 |
| HinfI | GANTC | 3 cut(s) | 398, 722, 969 |
| HpaII | CCGG | 4 cut(s) | 770, 896, 1128, 1390 |
| HphI | GGTGA | 1 cut(s) | 359 |
| Hpy166II | GTNNAC | 4 cut(s) | 397, 593, 925, 1147 |
| Hpy188I | TCNGA | 4 cut(s) | 268, 538, 557, 985 |
| Hpy188III | TCNNGA | 6 cut(s) | 550, 569, 754, 1090, 1113, 1390 |
| Hpy8I | GTNNAC | 4 cut(s) | 397, 593, 925, 1147 |
| HpyAV | CCTTC | 5 cut(s) | 251, 566, 769, 868, 1415 |
| HpyCH4III | ACNGT | 4 cut(s) | 277, 807, 1104, 1436 |
| HpyCH4IV | ACGT | 1 cut(s) | 1049 |
| HpyCH4V | TGCA | 6 cut(s) | 19, 653, 669, 1074, 1153, 1211 |
| HpyF3I | CTNAG | 2 cut(s) | 965, 1302 |
| HpySE526I | ACGT | 1 cut(s) | 1049 |
| HspAI | GCGC | 1 cut(s) | 814 |
| Kpn2I | TCCGGA | 1 cut(s) | 1389 |
| KpnI | GGTACC | 1 cut(s) | 770 |
| Ksp22I | TGATCA | 1 cut(s) | 546 |
| Kzo9I | GATC | 5 cut(s) | 32, 169, 325, 546, 552 |
| LmnI | GCTCC | 1 cut(s) | 302 |
| Lsp1109I | GCAGC | 1 cut(s) | 1165 |
| LweI | GCATC | 6 cut(s) | 6, 358, 892, 901, 1083, 1198 |
| MabI | ACCWGGT | 1 cut(s) | 586 |
| MaeI | CTAG | 4 cut(s) | 50, 90, 234, 957 |
| MaeII | ACGT | 1 cut(s) | 1049 |
| MaeIII | GTNAC | 6 cut(s) | 200, 575, 1045, 1297, 1307, 1398 |
| MalI | GATC | 5 cut(s) | 34, 171, 327, 548, 554 |
| MboI | GATC | 5 cut(s) | 32, 169, 325, 546, 552 |
| MboII | GAAGA | 3 cut(s) | 42, 302, 557 |
| MlsI | TGGCCA | 2 cut(s) | 937, 1033 |
| MluCI | AATT | 6 cut(s) | 62, 153, 734, 941, 1161, 1257 |
| MluNI | TGGCCA | 2 cut(s) | 937, 1033 |
| MlyI | GAGTC | 3 cut(s) | 392, 731, 963 |
| Mox20I | TGGCCA | 2 cut(s) | 937, 1033 |
| Mph1103I | ATGCAT | 4 cut(s) | 21, 655, 1076, 1213 |
| MroI | TCCGGA | 1 cut(s) | 1389 |
| MscI | TGGCCA | 2 cut(s) | 937, 1033 |
| MslI | CAYNNNNRTG | 4 cut(s) | 42, 861, 1069, 1142 |
| Msp20I | TGGCCA | 2 cut(s) | 937, 1033 |
| MspCI | CTTAAG | 1 cut(s) | 68 |
| MspI | CCGG | 4 cut(s) | 770, 896, 1128, 1390 |
| MspR9I | CCNGG | 4 cut(s) | 450, 588, 771, 1128 |
| Mva1269I | GAATGC | 2 cut(s) | 91, 253 |
| MvaI | CCWGG | 2 cut(s) | 450, 588 |
| NciI | CCSGG | 2 cut(s) | 771, 1128 |
| NcoI | CCATGG | 1 cut(s) | 1064 |
| NdeII | GATC | 5 cut(s) | 32, 169, 325, 546, 552 |
| NlaIV | GGNNCC | 3 cut(s) | 768, 962, 1117 |
| NmuCI | GTSAC | 1 cut(s) | 1045 |
| NsiI | ATGCAT | 4 cut(s) | 21, 655, 1076, 1213 |
| NspI | RCATGY | 9 cut(s) | 23, 531, 544, 653, 657, 692, 717, 820, 860 |
| PaeI | GCATGC | 2 cut(s) | 23, 860 |
| PagI | TCATGA | 2 cut(s) | 549, 1089 |
| PciI | ACATGT | 2 cut(s) | 540, 713 |
| PctI | GAATGC | 2 cut(s) | 91, 253 |
| PflFI | GACNNNGTC | 1 cut(s) | 749 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 944, 1277 |
| PkrI | GCNGC | 2 cut(s) | 891, 1155 |
| PleI | GAGTC | 3 cut(s) | 392, 730, 963 |
| PpsI | GAGTC | 3 cut(s) | 392, 730, 963 |
| Ppu21I | YACGTR | 1 cut(s) | 1050 |
| PscI | ACATGT | 2 cut(s) | 540, 713 |
| PshBI | ATTAAT | 2 cut(s) | 993, 1260 |
| Psp6I | CCWGG | 2 cut(s) | 448, 586 |
| PspEI | GGTNACC | 1 cut(s) | 1398 |
| PspFI | CCCAGC | 2 cut(s) | 371, 1270 |
| PspGI | CCWGG | 2 cut(s) | 448, 586 |
| PspN4I | GGNNCC | 3 cut(s) | 768, 962, 1117 |
| PspPI | GGNCC | 1 cut(s) | 1115 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1468 |
| PsyI | GACNNNGTC | 1 cut(s) | 749 |
| RsaI | GTAC | 5 cut(s) | 446, 457, 768, 788, 1148 |
| RsaNI | GTAC | 5 cut(s) | 445, 456, 767, 787, 1147 |
| RseI | CAYNNNNRTG | 4 cut(s) | 42, 861, 1069, 1142 |
| SatI | GCNGC | 2 cut(s) | 890, 1154 |
| Sau3AI | GATC | 5 cut(s) | 32, 169, 325, 546, 552 |
| Sau96I | GGNCC | 1 cut(s) | 1115 |
| SchI | GAGTC | 3 cut(s) | 392, 731, 963 |
| ScrFI | CCNGG | 4 cut(s) | 450, 588, 771, 1128 |
| SexAI | ACCWGGT | 1 cut(s) | 586 |
| SfaNI | GCATC | 6 cut(s) | 6, 358, 892, 901, 1083, 1198 |
| SfcI | CTRYAG | 2 cut(s) | 276, 1000 |
| SinI | GGWCC | 1 cut(s) | 1115 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 42, 861, 1069, 1142 |
| SmlI | CTYRAG | 1 cut(s) | 68 |
| SmoI | CTYRAG | 1 cut(s) | 68 |
| SphI | GCATGC | 2 cut(s) | 23, 860 |
| Sse9I | AATT | 6 cut(s) | 62, 153, 734, 941, 1161, 1257 |
| SsiI | CCGC | 1 cut(s) | 889 |
| SspI | AATATT | 1 cut(s) | 1022 |
| SspMI | CTAG | 4 cut(s) | 50, 90, 234, 957 |
| StyD4I | CCNGG | 4 cut(s) | 448, 586, 769, 1126 |
| StyI | CCWWGG | 2 cut(s) | 956, 1064 |
| TaaI | ACNGT | 4 cut(s) | 277, 807, 1104, 1436 |
| TaiI | ACGT | 1 cut(s) | 1052 |
| TaqI | TCGA | 7 cut(s) | 99, 366, 401, 423, 753, 827, 1419 |
| TasI | AATT | 6 cut(s) | 62, 153, 734, 941, 1161, 1257 |
| TatI | WGTACW | 1 cut(s) | 1146 |
| TauI | GCSGC | 1 cut(s) | 892 |
| TscAI | CASTG | 2 cut(s) | 1107, 1300 |
| TseFI | GTSAC | 1 cut(s) | 1045 |
| TseI | GCWGC | 1 cut(s) | 1153 |
| Tsp45I | GTSAC | 1 cut(s) | 1045 |
| TspDTI | ATGAA | 7 cut(s) | 260, 270, 428, 612, 879, 1078, 1091 |
| TspGWI | ACGGA | 1 cut(s) | 237 |
| TspRI | CASTG | 2 cut(s) | 1107, 1300 |
| Tth111I | GACNNNGTC | 1 cut(s) | 749 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 944, 1277 |
| Vha464I | CTTAAG | 1 cut(s) | 68 |
| VpaK11BI | GGWCC | 1 cut(s) | 1115 |
| VspI | ATTAAT | 2 cut(s) | 993, 1260 |
| XapI | RAATTY | 2 cut(s) | 62, 941 |
| XceI | RCATGY | 9 cut(s) | 23, 531, 544, 653, 657, 692, 717, 820, 860 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 613 |
| XmaJI | CCTAGG | 1 cut(s) | 956 |
| XmiI | GTMKAC | 2 cut(s) | 396, 924 |
| XspI | CTAG | 4 cut(s) | 50, 90, 234, 957 |
| Zsp2I | ATGCAT | 4 cut(s) | 21, 655, 1076, 1213 |