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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 3 cut(s) | 714, 756, 969 |
| AccII | CGCG | 1 cut(s) | 936 |
| AciI | CCGC | 1 cut(s) | 1417 |
| AclWI | GGATC | 6 cut(s) | 99, 727, 740, 1084, 1097, 1244 |
| AcoI | YGGCCR | 1 cut(s) | 644 |
| AcuI | CTGAAG | 1 cut(s) | 255 |
| AfaI | GTAC | 4 cut(s) | 357, 494, 605, 882 |
| AfeI | AGCGCT | 1 cut(s) | 155 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 770 |
| AgeI | ACCGGT | 1 cut(s) | 568 |
| AjnI | CCWGG | 2 cut(s) | 55, 261 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 71, 103, 1302, 1334 |
| AleI | CACNNNNGTG | 1 cut(s) | 1199 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1200, 1232 |
| AluBI | AGCT | 6 cut(s) | 171, 740, 998, 1069, 1147, 1318 |
| AluI | AGCT | 6 cut(s) | 171, 740, 998, 1069, 1147, 1318 |
| Alw21I | GWGCWC | 1 cut(s) | 1071 |
| AlwI | GGATC | 6 cut(s) | 99, 727, 740, 1084, 1097, 1244 |
| Aor51HI | AGCGCT | 1 cut(s) | 155 |
| AoxI | GGCC | 3 cut(s) | 644, 742, 1240 |
| ApeKI | GCWGC | 2 cut(s) | 417, 442 |
| AsiGI | ACCGGT | 1 cut(s) | 568 |
| AspLEI | GCGC | 2 cut(s) | 156, 936 |
| AspS9I | GGNCC | 5 cut(s) | 39, 140, 566, 743, 1241 |
| AsuHPI | GGTGA | 4 cut(s) | 88, 530, 1331, 1342 |
| AvaII | GGWCC | 3 cut(s) | 39, 140, 566 |
| AxyI | CCTNAGG | 1 cut(s) | 769 |
| BamHI | GGATCC | 2 cut(s) | 732, 1089 |
| BanII | GRGCYC | 1 cut(s) | 1071 |
| Bbv12I | GWGCWC | 1 cut(s) | 1071 |
| BbvCI | CCTCAGC | 1 cut(s) | 678 |
| BbvI | GCAGC | 2 cut(s) | 429, 429 |
| BccI | CCATC | 6 cut(s) | 65, 353, 511, 1350, 1372, 1439 |
| BciT130I | CCWGG | 2 cut(s) | 57, 263 |
| BciVI | GTATCC | 1 cut(s) | 600 |
| BfaI | CTAG | 4 cut(s) | 95, 485, 542, 1284 |
| BfmI | CTRYAG | 3 cut(s) | 415, 1042, 1245 |
| BfoI | RGCGCY | 1 cut(s) | 157 |
| BfuI | GTATCC | 1 cut(s) | 600 |
| BglII | AGATCT | 1 cut(s) | 612 |
| BisI | GCNGC | 2 cut(s) | 418, 443 |
| BlsI | GCNGC | 2 cut(s) | 419, 444 |
| BmcAI | AGTACT | 1 cut(s) | 494 |
| Bme1390I | CCNGG | 2 cut(s) | 57, 263 |
| Bme18I | GGWCC | 3 cut(s) | 39, 140, 566 |
| BmgT120I | GGNCC | 5 cut(s) | 39, 140, 566, 743, 1241 |
| BmiI | GGNNCC | 5 cut(s) | 40, 142, 231, 734, 1091 |
| BmrFI | CCNGG | 2 cut(s) | 57, 263 |
| BmsI | GCATC | 4 cut(s) | 188, 793, 817, 1350 |
| Bpu10I | CCTNAGC | 1 cut(s) | 678 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1094 |
| BsaJI | CCNNGG | 1 cut(s) | 349 |
| BsaWI | WCCGGW | 1 cut(s) | 568 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 318, 348, 677, 707 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 770 |
| Bse118I | RCCGGY | 2 cut(s) | 367, 568 |
| Bse1I | ACTGG | 2 cut(s) | 916, 1006 |
| Bse21I | CCTNAGG | 1 cut(s) | 769 |
| Bse3DI | GCAATG | 2 cut(s) | 657, 880 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1094 |
| BseBI | CCWGG | 2 cut(s) | 57, 263 |
| BseDI | CCNNGG | 1 cut(s) | 349 |
| BseGI | GGATG | 7 cut(s) | 76, 280, 345, 503, 591, 834, 1342 |
| BseJI | GATNNNNATC | 1 cut(s) | 1094 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 770 |
| BseMI | GCAATG | 2 cut(s) | 657, 880 |
| BseMII | CTCAG | 4 cut(s) | 654, 669, 783, 831 |
| BseNI | ACTGG | 2 cut(s) | 916, 1006 |
| BseRI | GAGGAG | 4 cut(s) | 323, 695, 698, 1442 |
| BseXI | GCAGC | 2 cut(s) | 429, 429 |
| BseYI | CCCAGC | 1 cut(s) | 736 |
| Bsh1236I | CGCG | 1 cut(s) | 936 |
| BshFI | GGCC | 3 cut(s) | 646, 744, 1242 |
| BshTI | ACCGGT | 1 cut(s) | 568 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1071 |
| BsiSI | CCGG | 2 cut(s) | 368, 569 |
| BslFI | GGGAC | 3 cut(s) | 52, 126, 1337 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 770 |
| BsmFI | GGGAC | 3 cut(s) | 52, 126, 1337 |
| BsmI | GAATGC | 1 cut(s) | 1281 |
| BsnI | GGCC | 3 cut(s) | 646, 744, 1242 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1071 |
| Bsp143I | GATC | 8 cut(s) | 91, 130, 612, 732, 947, 1089, 1095, 1249 |
| BspACI | CCGC | 1 cut(s) | 1417 |
| BspANI | GGCC | 3 cut(s) | 646, 744, 1242 |
| BspCNI | CTCAG | 4 cut(s) | 655, 670, 782, 832 |
| BspFNI | CGCG | 1 cut(s) | 936 |
| BspLI | GGNNCC | 5 cut(s) | 40, 142, 231, 734, 1091 |
| BspMAI | CTGCAG | 1 cut(s) | 419 |
| BspPI | GGATC | 6 cut(s) | 99, 727, 740, 1084, 1097, 1244 |
| BsrDI | GCAATG | 2 cut(s) | 657, 880 |
| BsrFI | RCCGGY | 2 cut(s) | 367, 568 |
| BsrI | ACTGG | 2 cut(s) | 916, 1006 |
| BssAI | RCCGGY | 2 cut(s) | 367, 568 |
| BssECI | CCNNGG | 1 cut(s) | 349 |
| BssMI | GATC | 8 cut(s) | 91, 130, 612, 732, 947, 1089, 1095, 1249 |
| Bst2UI | CCWGG | 2 cut(s) | 57, 263 |
| Bst4CI | ACNGT | 6 cut(s) | 535, 754, 962, 1214, 1295, 1330 |
| BstC8I | GCNNGC | 4 cut(s) | 158, 169, 742, 978 |
| BstDEI | CTNAG | 9 cut(s) | 287, 663, 678, 769, 815, 840, 981, 1271, 1387 |
| BstDSI | CCRYGG | 1 cut(s) | 349 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 768 |
| BstF5I | GGATG | 7 cut(s) | 76, 280, 345, 503, 591, 834, 1342 |
| BstFNI | CGCG | 1 cut(s) | 936 |
| BstH2I | RGCGCY | 1 cut(s) | 157 |
| BstHHI | GCGC | 2 cut(s) | 156, 936 |
| BstKTI | GATC | 8 cut(s) | 94, 133, 615, 735, 950, 1092, 1098, 1252 |
| BstMBI | GATC | 8 cut(s) | 91, 130, 612, 732, 947, 1089, 1095, 1249 |
| BstNI | CCWGG | 2 cut(s) | 57, 263 |
| BstNSI | RCATGY | 2 cut(s) | 205, 1417 |
| BstSCI | CCNGG | 2 cut(s) | 55, 261 |
| BstSFI | CTRYAG | 3 cut(s) | 415, 1042, 1245 |
| BstUI | CGCG | 1 cut(s) | 936 |
| BstV1I | GCAGC | 2 cut(s) | 429, 429 |
| BstX2I | RGATCY | 5 cut(s) | 91, 612, 732, 1089, 1249 |
| BstYI | RGATCY | 5 cut(s) | 91, 612, 732, 1089, 1249 |
| Bsu36I | CCTNAGG | 1 cut(s) | 769 |
| BsuI | GTATCC | 1 cut(s) | 600 |
| BsuRI | GGCC | 3 cut(s) | 646, 744, 1242 |
| BtgI | CCRYGG | 1 cut(s) | 349 |
| BtgZI | GCGATG | 1 cut(s) | 951 |
| BtsCI | GGATG | 7 cut(s) | 76, 280, 345, 503, 591, 834, 1342 |
| BtsIMutI | CAGTG | 3 cut(s) | 898, 967, 1326 |
| Cac8I | GCNNGC | 4 cut(s) | 158, 169, 742, 978 |
| CfoI | GCGC | 2 cut(s) | 156, 936 |
| Cfr10I | RCCGGY | 2 cut(s) | 367, 568 |
| Cfr13I | GGNCC | 5 cut(s) | 39, 140, 566, 743, 1241 |
| CseI | GACGC | 1 cut(s) | 961 |
| CsiI | ACCWGGT | 1 cut(s) | 261 |
| Csp6I | GTAC | 4 cut(s) | 356, 493, 604, 881 |
| CspAI | ACCGGT | 1 cut(s) | 568 |
| CviAII | CATG | 7 cut(s) | 202, 305, 314, 338, 607, 1379, 1414 |
| CviQI | GTAC | 4 cut(s) | 356, 493, 604, 881 |
| DdeI | CTNAG | 9 cut(s) | 287, 663, 678, 769, 815, 840, 981, 1271, 1387 |
| DpnI | GATC | 8 cut(s) | 93, 132, 614, 734, 949, 1091, 1097, 1251 |
| DpnII | GATC | 8 cut(s) | 91, 130, 612, 732, 947, 1089, 1095, 1249 |
| DraI | TTTAAA | 1 cut(s) | 1188 |
| EaeI | YGGCCR | 1 cut(s) | 644 |
| Ecl136II | GAGCTC | 1 cut(s) | 1069 |
| Eco24I | GRGCYC | 1 cut(s) | 1071 |
| Eco47I | GGWCC | 3 cut(s) | 39, 140, 566 |
| Eco47III | AGCGCT | 1 cut(s) | 155 |
| Eco53kI | GAGCTC | 1 cut(s) | 1069 |
| Eco57I | CTGAAG | 1 cut(s) | 255 |
| Eco81I | CCTNAGG | 1 cut(s) | 769 |
| EcoICRI | GAGCTC | 1 cut(s) | 1069 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 768 |
| EcoO109I | RGGNCCY | 3 cut(s) | 39, 140, 1241 |
| EcoRII | CCWGG | 2 cut(s) | 55, 261 |
| EcoT22I | ATGCAT | 2 cut(s) | 6, 203 |
| EcoT38I | GRGCYC | 1 cut(s) | 1071 |
| FaeI | CATG | 7 cut(s) | 205, 308, 317, 341, 610, 1382, 1417 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 140, 172, 1302, 1334 |
| FaqI | GGGAC | 3 cut(s) | 52, 126, 1337 |
| FatI | CATG | 7 cut(s) | 201, 304, 313, 337, 606, 1378, 1413 |
| FauI | CCCGC | 1 cut(s) | 1410 |
| FblI | GTMKAC | 3 cut(s) | 714, 756, 969 |
| Fnu4HI | GCNGC | 2 cut(s) | 418, 443 |
| FokI | GGATG | 7 cut(s) | 83, 287, 332, 490, 598, 821, 1329 |
| FriOI | GRGCYC | 1 cut(s) | 1071 |
| Fsp4HI | GCNGC | 2 cut(s) | 418, 443 |
| FspBI | CTAG | 4 cut(s) | 95, 485, 542, 1284 |
| GlaI | GCGC | 2 cut(s) | 155, 935 |
| GluI | GCNGC | 2 cut(s) | 418, 443 |
| GsaI | CCCAGC | 1 cut(s) | 740 |
| HaeII | RGCGCY | 1 cut(s) | 157 |
| HaeIII | GGCC | 3 cut(s) | 646, 744, 1242 |
| HapII | CCGG | 2 cut(s) | 368, 569 |
| HgaI | GACGC | 1 cut(s) | 961 |
| HhaI | GCGC | 2 cut(s) | 156, 936 |
| Hin1II | CATG | 7 cut(s) | 205, 308, 317, 341, 610, 1382, 1417 |
| Hin6I | GCGC | 2 cut(s) | 154, 934 |
| HinP1I | GCGC | 2 cut(s) | 154, 934 |
| HincII | GTYRAC | 1 cut(s) | 715 |
| HindII | GTYRAC | 1 cut(s) | 715 |
| HinfI | GANTC | 1 cut(s) | 912 |
| HpaII | CCGG | 2 cut(s) | 368, 569 |
| HphI | GGTGA | 4 cut(s) | 88, 530, 1331, 1342 |
| Hpy166II | GTNNAC | 9 cut(s) | 293, 356, 538, 566, 715, 757, 821, 970, 1155 |
| Hpy188I | TCNGA | 2 cut(s) | 374, 862 |
| Hpy188III | TCNNGA | 4 cut(s) | 134, 233, 598, 1093 |
| Hpy8I | GTNNAC | 9 cut(s) | 293, 356, 538, 566, 715, 757, 821, 970, 1155 |
| Hpy99I | CGWCG | 3 cut(s) | 716, 719, 955 |
| HpyAV | CCTTC | 8 cut(s) | 131, 142, 220, 230, 604, 644, 1129, 1415 |
| HpyCH4III | ACNGT | 6 cut(s) | 535, 754, 962, 1214, 1295, 1330 |
| HpyCH4IV | ACGT | 2 cut(s) | 602, 1157 |
| HpyCH4V | TGCA | 7 cut(s) | 4, 201, 325, 417, 618, 657, 908 |
| HpyF3I | CTNAG | 9 cut(s) | 287, 663, 678, 769, 815, 840, 981, 1271, 1387 |
| HpySE526I | ACGT | 2 cut(s) | 602, 1157 |
| Hsp92II | CATG | 7 cut(s) | 205, 308, 317, 341, 610, 1382, 1417 |
| HspAI | GCGC | 2 cut(s) | 154, 934 |
| Kzo9I | GATC | 8 cut(s) | 91, 130, 612, 732, 947, 1089, 1095, 1249 |
| LmnI | GCTCC | 2 cut(s) | 151, 1066 |
| Lsp1109I | GCAGC | 2 cut(s) | 429, 429 |
| LweI | GCATC | 4 cut(s) | 188, 793, 817, 1350 |
| MabI | ACCWGGT | 1 cut(s) | 261 |
| MaeI | CTAG | 4 cut(s) | 95, 485, 542, 1284 |
| MaeII | ACGT | 2 cut(s) | 602, 1157 |
| MaeIII | GTNAC | 6 cut(s) | 479, 896, 1295, 1374, 1382, 1409 |
| MalI | GATC | 8 cut(s) | 93, 132, 614, 734, 949, 1091, 1097, 1251 |
| MboI | GATC | 8 cut(s) | 91, 130, 612, 732, 947, 1089, 1095, 1249 |
| MboII | GAAGA | 3 cut(s) | 237, 622, 1322 |
| MfeI | CAATTG | 1 cut(s) | 780 |
| MflI | RGATCY | 5 cut(s) | 91, 612, 732, 1089, 1249 |
| MhlI | GDGCHC | 1 cut(s) | 1071 |
| MluCI | AATT | 6 cut(s) | 375, 780, 903, 1020, 1165, 1437 |
| MlyI | GAGTC | 1 cut(s) | 921 |
| MmeI | TCCRAC | 1 cut(s) | 69 |
| Mph1103I | ATGCAT | 2 cut(s) | 6, 203 |
| MseI | TTAA | 7 cut(s) | 179, 281, 297, 320, 405, 1101, 1187 |
| MslI | CAYNNNNRTG | 4 cut(s) | 1188, 1199, 1355, 1358 |
| MspA1I | CMGCKG | 1 cut(s) | 740 |
| MspI | CCGG | 2 cut(s) | 368, 569 |
| MspR9I | CCNGG | 2 cut(s) | 57, 263 |
| MunI | CAATTG | 1 cut(s) | 780 |
| Mva1269I | GAATGC | 1 cut(s) | 1281 |
| MvaI | CCWGG | 2 cut(s) | 57, 263 |
| MvnI | CGCG | 1 cut(s) | 936 |
| NdeII | GATC | 8 cut(s) | 91, 130, 612, 732, 947, 1089, 1095, 1249 |
| NlaIII | CATG | 7 cut(s) | 205, 308, 317, 341, 610, 1382, 1417 |
| NlaIV | GGNNCC | 5 cut(s) | 40, 142, 231, 734, 1091 |
| NmuCI | GTSAC | 1 cut(s) | 479 |
| NsiI | ATGCAT | 2 cut(s) | 6, 203 |
| NspI | RCATGY | 2 cut(s) | 205, 1417 |
| OliI | CACNNNNGTG | 1 cut(s) | 1199 |
| PctI | GAATGC | 1 cut(s) | 1281 |
| PfoI | TCCNGGA | 1 cut(s) | 55 |
| PinAI | ACCGGT | 1 cut(s) | 568 |
| PkrI | GCNGC | 2 cut(s) | 419, 444 |
| PleI | GAGTC | 1 cut(s) | 920 |
| PpsI | GAGTC | 1 cut(s) | 920 |
| PpuMI | RGGWCCY | 2 cut(s) | 39, 140 |
| Psp124BI | GAGCTC | 1 cut(s) | 1071 |
| Psp5II | RGGWCCY | 2 cut(s) | 39, 140 |
| Psp6I | CCWGG | 2 cut(s) | 55, 261 |
| PspFI | CCCAGC | 1 cut(s) | 736 |
| PspGI | CCWGG | 2 cut(s) | 55, 261 |
| PspN4I | GGNNCC | 5 cut(s) | 40, 142, 231, 734, 1091 |
| PspPI | GGNCC | 5 cut(s) | 39, 140, 566, 743, 1241 |
| PspPPI | RGGWCCY | 2 cut(s) | 39, 140 |
| PstI | CTGCAG | 1 cut(s) | 419 |
| PsuI | RGATCY | 5 cut(s) | 91, 612, 732, 1089, 1249 |
| PvuII | CAGCTG | 1 cut(s) | 740 |
| RsaI | GTAC | 4 cut(s) | 357, 494, 605, 882 |
| RsaNI | GTAC | 4 cut(s) | 356, 493, 604, 881 |
| RseI | CAYNNNNRTG | 4 cut(s) | 1188, 1199, 1355, 1358 |
| SacI | GAGCTC | 1 cut(s) | 1071 |
| SalI | GTCGAC | 1 cut(s) | 713 |
| SaqAI | TTAA | 7 cut(s) | 179, 281, 297, 320, 405, 1101, 1187 |
| SatI | GCNGC | 2 cut(s) | 418, 443 |
| Sau3AI | GATC | 8 cut(s) | 91, 130, 612, 732, 947, 1089, 1095, 1249 |
| Sau96I | GGNCC | 5 cut(s) | 39, 140, 566, 743, 1241 |
| ScaI | AGTACT | 1 cut(s) | 494 |
| SchI | GAGTC | 1 cut(s) | 921 |
| ScrFI | CCNGG | 2 cut(s) | 57, 263 |
| SduI | GDGCHC | 1 cut(s) | 1071 |
| SexAI | ACCWGGT | 1 cut(s) | 261 |
| SfaNI | GCATC | 4 cut(s) | 188, 793, 817, 1350 |
| SfcI | CTRYAG | 3 cut(s) | 415, 1042, 1245 |
| SgrDI | CGTCGACG | 1 cut(s) | 713 |
| SinI | GGWCC | 3 cut(s) | 39, 140, 566 |
| SmiI | ATTTAAAT | 1 cut(s) | 1188 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 1188, 1199, 1355, 1358 |
| Sse9I | AATT | 6 cut(s) | 375, 780, 903, 1020, 1165, 1437 |
| SsiI | CCGC | 1 cut(s) | 1417 |
| SspI | AATATT | 1 cut(s) | 52 |
| SspMI | CTAG | 4 cut(s) | 95, 485, 542, 1284 |
| SstI | GAGCTC | 1 cut(s) | 1071 |
| StyD4I | CCNGG | 2 cut(s) | 55, 261 |
| SwaI | ATTTAAAT | 1 cut(s) | 1188 |
| TaaI | ACNGT | 6 cut(s) | 535, 754, 962, 1214, 1295, 1330 |
| TaiI | ACGT | 2 cut(s) | 605, 1160 |
| TaqI | TCGA | 5 cut(s) | 99, 135, 714, 950, 1071 |
| TasI | AATT | 6 cut(s) | 375, 780, 903, 1020, 1165, 1437 |
| TatI | WGTACW | 2 cut(s) | 355, 492 |
| Tru1I | TTAA | 7 cut(s) | 179, 281, 297, 320, 405, 1101, 1187 |
| Tru9I | TTAA | 7 cut(s) | 179, 281, 297, 320, 405, 1101, 1187 |
| TscAI | CASTG | 3 cut(s) | 898, 967, 1333 |
| TseFI | GTSAC | 1 cut(s) | 479 |
| TseI | GCWGC | 2 cut(s) | 417, 442 |
| Tsp45I | GTSAC | 1 cut(s) | 479 |
| TspDTI | ATGAA | 7 cut(s) | 204, 239, 474, 515, 623, 891, 1092 |
| TspGWI | ACGGA | 1 cut(s) | 338 |
| TspRI | CASTG | 3 cut(s) | 898, 967, 1333 |
| VpaK11BI | GGWCC | 3 cut(s) | 39, 140, 566 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 768 |
| XceI | RCATGY | 2 cut(s) | 205, 1417 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 168 |
| XmiI | GTMKAC | 3 cut(s) | 714, 756, 969 |
| XspI | CTAG | 4 cut(s) | 95, 485, 542, 1284 |
| ZrmI | AGTACT | 1 cut(s) | 494 |
| Zsp2I | ATGCAT | 2 cut(s) | 6, 203 |