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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 2 cut(s) | 113, 173 |
| AciI | CCGC | 6 cut(s) | 46, 100, 111, 171, 188, 331 |
| AclI | AACGTT | 1 cut(s) | 294 |
| AclWI | GGATC | 1 cut(s) | 12 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 45, 219, 238, 337 |
| AgsI | TTSAA | 1 cut(s) | 300 |
| AluBI | AGCT | 1 cut(s) | 273 |
| AluI | AGCT | 1 cut(s) | 273 |
| Alw26I | GTCTC | 1 cut(s) | 54 |
| AlwI | GGATC | 1 cut(s) | 12 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 252 |
| Ama87I | CYCGRG | 2 cut(s) | 114, 145 |
| AspLEI | GCGC | 1 cut(s) | 38 |
| AspS9I | GGNCC | 1 cut(s) | 222 |
| AsuC2I | CCSGG | 2 cut(s) | 220, 350 |
| AsuHPI | GGTGA | 1 cut(s) | 208 |
| AvaI | CYCGRG | 2 cut(s) | 114, 145 |
| AvaII | GGWCC | 1 cut(s) | 222 |
| BbsI | GAAGAC | 1 cut(s) | 129 |
| BccI | CCATC | 3 cut(s) | 67, 221, 236 |
| BcnI | CCSGG | 2 cut(s) | 220, 350 |
| BcoDI | GTCTC | 1 cut(s) | 54 |
| BfaI | CTAG | 1 cut(s) | 18 |
| BfoI | RGCGCY | 1 cut(s) | 39 |
| Bme1390I | CCNGG | 2 cut(s) | 220, 350 |
| Bme18I | GGWCC | 1 cut(s) | 222 |
| BmeT110I | CYCGRG | 2 cut(s) | 114, 145 |
| BmgT120I | GGNCC | 1 cut(s) | 222 |
| BmiI | GGNNCC | 1 cut(s) | 31 |
| BmrFI | CCNGG | 2 cut(s) | 220, 350 |
| BpiI | GAAGAC | 1 cut(s) | 129 |
| BpmI | CTGGAG | 2 cut(s) | 16, 71 |
| BpuEI | CTTGAG | 1 cut(s) | 159 |
| BpuMI | CCSGG | 2 cut(s) | 220, 350 |
| BsaI | GGTCTC | 1 cut(s) | 54 |
| BsaJI | CCNNGG | 1 cut(s) | 281 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 45, 219, 238, 337 |
| Bse118I | RCCGGY | 1 cut(s) | 262 |
| Bse3DI | GCAATG | 1 cut(s) | 109 |
| BseDI | CCNNGG | 1 cut(s) | 281 |
| BseGI | GGATG | 2 cut(s) | 78, 247 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 45, 219, 238, 337 |
| BseMI | GCAATG | 1 cut(s) | 109 |
| BseMII | CTCAG | 3 cut(s) | 56, 123, 335 |
| BseRI | GAGGAG | 2 cut(s) | 298, 319 |
| BsiHKCI | CYCGRG | 2 cut(s) | 114, 145 |
| BsiSI | CCGG | 3 cut(s) | 220, 263, 350 |
| BslFI | GGGAC | 1 cut(s) | 332 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 45, 219, 238, 337 |
| BsmAI | GTCTC | 1 cut(s) | 54 |
| BsmFI | GGGAC | 1 cut(s) | 332 |
| Bso31I | GGTCTC | 1 cut(s) | 54 |
| BsoBI | CYCGRG | 2 cut(s) | 114, 145 |
| Bsp143I | GATC | 2 cut(s) | 4, 287 |
| BspACI | CCGC | 6 cut(s) | 46, 100, 111, 171, 188, 331 |
| BspCNI | CTCAG | 3 cut(s) | 55, 124, 334 |
| BspLI | GGNNCC | 1 cut(s) | 31 |
| BspPI | GGATC | 1 cut(s) | 12 |
| BspTNI | GGTCTC | 1 cut(s) | 54 |
| BsrBI | CCGCTC | 2 cut(s) | 113, 173 |
| BsrDI | GCAATG | 1 cut(s) | 109 |
| BsrFI | RCCGGY | 1 cut(s) | 262 |
| BssAI | RCCGGY | 1 cut(s) | 262 |
| BssECI | CCNNGG | 1 cut(s) | 281 |
| BssMI | GATC | 2 cut(s) | 4, 287 |
| BstDEI | CTNAG | 3 cut(s) | 42, 132, 321 |
| BstF5I | GGATG | 2 cut(s) | 78, 247 |
| BstH2I | RGCGCY | 1 cut(s) | 39 |
| BstHHI | GCGC | 1 cut(s) | 38 |
| BstKTI | GATC | 2 cut(s) | 7, 290 |
| BstMAI | GTCTC | 1 cut(s) | 54 |
| BstMBI | GATC | 2 cut(s) | 4, 287 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 27 |
| BstSCI | CCNGG | 2 cut(s) | 218, 348 |
| BstV2I | GAAGAC | 1 cut(s) | 129 |
| BtsCI | GGATG | 2 cut(s) | 78, 247 |
| BtsIMutI | CAGTG | 1 cut(s) | 187 |
| CaiI | CAGNNNCTG | 1 cut(s) | 252 |
| CfoI | GCGC | 1 cut(s) | 38 |
| Cfr10I | RCCGGY | 1 cut(s) | 262 |
| Cfr13I | GGNCC | 1 cut(s) | 222 |
| CviJI | RGCY | 5 cut(s) | 30, 119, 206, 262, 273 |
| CviKI_1 | RGCY | 5 cut(s) | 30, 119, 206, 262, 273 |
| DdeI | CTNAG | 3 cut(s) | 42, 132, 321 |
| DpnI | GATC | 2 cut(s) | 6, 289 |
| DpnII | GATC | 2 cut(s) | 4, 287 |
| Eco31I | GGTCTC | 1 cut(s) | 54 |
| Eco32I | GATATC | 1 cut(s) | 83 |
| Eco47I | GGWCC | 1 cut(s) | 222 |
| Eco88I | CYCGRG | 2 cut(s) | 114, 145 |
| EcoRV | GATATC | 1 cut(s) | 83 |
| FaiI | YATR | 2 cut(s) | 9, 209 |
| FaqI | GGGAC | 1 cut(s) | 332 |
| FauI | CCCGC | 5 cut(s) | 107, 118, 178, 181, 338 |
| FokI | GGATG | 2 cut(s) | 85, 254 |
| FspBI | CTAG | 1 cut(s) | 18 |
| GlaI | GCGC | 1 cut(s) | 37 |
| GsuI | CTGGAG | 2 cut(s) | 16, 71 |
| HaeII | RGCGCY | 1 cut(s) | 39 |
| HapII | CCGG | 3 cut(s) | 220, 263, 350 |
| HhaI | GCGC | 1 cut(s) | 38 |
| Hin6I | GCGC | 1 cut(s) | 36 |
| HinP1I | GCGC | 1 cut(s) | 36 |
| HinfI | GANTC | 2 cut(s) | 134, 148 |
| HpaII | CCGG | 3 cut(s) | 220, 263, 350 |
| HphI | GGTGA | 1 cut(s) | 208 |
| Hpy188I | TCNGA | 2 cut(s) | 133, 324 |
| Hpy188III | TCNNGA | 1 cut(s) | 152 |
| Hpy99I | CGWCG | 1 cut(s) | 68 |
| HpyAV | CCTTC | 1 cut(s) | 71 |
| HpyCH4IV | ACGT | 2 cut(s) | 294, 345 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 27 |
| HpyF3I | CTNAG | 3 cut(s) | 42, 132, 321 |
| HpySE526I | ACGT | 2 cut(s) | 294, 345 |
| HspAI | GCGC | 1 cut(s) | 36 |
| Kzo9I | GATC | 2 cut(s) | 4, 287 |
| LmnI | GCTCC | 1 cut(s) | 35 |
| LpnPI | CCDG | 6 cut(s) | 46, 101, 165, 232, 233, 276 |
| MaeI | CTAG | 1 cut(s) | 18 |
| MaeII | ACGT | 2 cut(s) | 294, 345 |
| MalI | GATC | 2 cut(s) | 6, 289 |
| MbiI | CCGCTC | 2 cut(s) | 113, 173 |
| MboI | GATC | 2 cut(s) | 4, 287 |
| MboII | GAAGA | 2 cut(s) | 114, 129 |
| MluCI | AATT | 1 cut(s) | 355 |
| MlyI | GAGTC | 2 cut(s) | 143, 157 |
| MnlI | CCTC | 7 cut(s) | 49, 139, 196, 232, 276, 297, 337 |
| MslI | CAYNNNNRTG | 1 cut(s) | 162 |
| MspA1I | CMGCKG | 2 cut(s) | 46, 333 |
| MspI | CCGG | 3 cut(s) | 220, 263, 350 |
| MspR9I | CCNGG | 2 cut(s) | 220, 350 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 27 |
| NciI | CCSGG | 2 cut(s) | 220, 350 |
| NdeII | GATC | 2 cut(s) | 4, 287 |
| NlaIV | GGNNCC | 1 cut(s) | 31 |
| PaeR7I | CTCGAG | 1 cut(s) | 145 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 60 |
| PfoI | TCCNGGA | 1 cut(s) | 348 |
| PleI | GAGTC | 2 cut(s) | 142, 156 |
| PpsI | GAGTC | 2 cut(s) | 142, 156 |
| Psp1406I | AACGTT | 1 cut(s) | 294 |
| PspN4I | GGNNCC | 1 cut(s) | 31 |
| PspPI | GGNCC | 1 cut(s) | 222 |
| PspXI | VCTCGAGB | 1 cut(s) | 145 |
| PstNI | CAGNNNCTG | 1 cut(s) | 252 |
| RseI | CAYNNNNRTG | 1 cut(s) | 162 |
| Sau3AI | GATC | 2 cut(s) | 4, 287 |
| Sau96I | GGNCC | 1 cut(s) | 222 |
| SchI | GAGTC | 2 cut(s) | 143, 157 |
| ScrFI | CCNGG | 2 cut(s) | 220, 350 |
| SetI | ASST | 6 cut(s) | 97, 227, 275, 297, 329, 348 |
| Sfr274I | CTCGAG | 1 cut(s) | 145 |
| SinI | GGWCC | 1 cut(s) | 222 |
| SlaI | CTCGAG | 1 cut(s) | 145 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 162 |
| SmlI | CTYRAG | 2 cut(s) | 145, 174 |
| SmoI | CTYRAG | 2 cut(s) | 145, 174 |
| Sse9I | AATT | 1 cut(s) | 355 |
| SsiI | CCGC | 6 cut(s) | 46, 100, 111, 171, 188, 331 |
| SspMI | CTAG | 1 cut(s) | 18 |
| StyD4I | CCNGG | 2 cut(s) | 218, 348 |
| TaiI | ACGT | 2 cut(s) | 297, 348 |
| TaqI | TCGA | 1 cut(s) | 146 |
| TasI | AATT | 1 cut(s) | 355 |
| TscAI | CASTG | 1 cut(s) | 187 |
| TspRI | CASTG | 1 cut(s) | 187 |
| VpaK11BI | GGWCC | 1 cut(s) | 222 |
| XhoI | CTCGAG | 1 cut(s) | 145 |
| XspI | CTAG | 1 cut(s) | 18 |