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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1771 |
| AatII | GACGTC | 1 cut(s) | 1179 |
| Acc36I | ACCTGC | 1 cut(s) | 1771 |
| Acc65I | GGTACC | 4 cut(s) | 158, 252, 908, 1401 |
| AccB1I | GGYRCC | 6 cut(s) | 158, 252, 591, 908, 1401, 2192 |
| AccBSI | CCGCTC | 1 cut(s) | 1825 |
| AccI | GTMKAC | 2 cut(s) | 447, 1179 |
| AccII | CGCG | 5 cut(s) | 156, 726, 728, 1281, 1286 |
| AclWI | GGATC | 5 cut(s) | 211, 331, 1253, 1572, 1995 |
| AcoI | YGGCCR | 7 cut(s) | 351, 513, 675, 1299, 1848, 2109, 2181 |
| AcsI | RAATTY | 4 cut(s) | 68, 88, 1541, 2132 |
| AcuI | CTGAAG | 4 cut(s) | 479, 1589, 1632, 1772 |
| AcyI | GRCGYC | 3 cut(s) | 1176, 1266, 1728 |
| AgeI | ACCGGT | 3 cut(s) | 249, 905, 1404 |
| AgsI | TTSAA | 2 cut(s) | 88, 1711 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1186 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1796, 1828 |
| AleI | CACNNNNGTG | 1 cut(s) | 1328 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 257, 289 |
| Alw21I | GWGCWC | 2 cut(s) | 1010, 1644 |
| Alw26I | GTCTC | 6 cut(s) | 1211, 1634, 1682, 1960, 2150, 2208 |
| AlwI | GGATC | 5 cut(s) | 211, 331, 1253, 1572, 1995 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 604, 1513, 1949 |
| Ama87I | CYCGRG | 6 cut(s) | 529, 972, 1433, 1635, 1683, 1920 |
| ApoI | RAATTY | 4 cut(s) | 68, 88, 1541, 2132 |
| AsiGI | ACCGGT | 3 cut(s) | 249, 905, 1404 |
| Asp718I | GGTACC | 4 cut(s) | 158, 252, 908, 1401 |
| AspA2I | CCTAGG | 1 cut(s) | 256 |
| AspLEI | GCGC | 5 cut(s) | 479, 544, 728, 1283, 1288 |
| AspS9I | GGNCC | 9 cut(s) | 151, 273, 334, 399, 685, 928, 1437, 1443, 1551 |
| AsuHPI | GGTGA | 8 cut(s) | 618, 787, 1262, 1422, 1624, 1672, 1795, 1895 |
| AvaI | CYCGRG | 6 cut(s) | 529, 972, 1433, 1635, 1683, 1920 |
| AvaII | GGWCC | 6 cut(s) | 151, 273, 399, 685, 928, 1437 |
| AvrII | CCTAGG | 1 cut(s) | 256 |
| AxyI | CCTNAGG | 1 cut(s) | 360 |
| BaeGI | GKGCMC | 2 cut(s) | 1356, 1705 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 150, 183, 244, 277 |
| BalI | TGGCCA | 3 cut(s) | 353, 677, 1850 |
| BanI | GGYRCC | 6 cut(s) | 158, 252, 591, 908, 1401, 2192 |
| BanII | GRGCYC | 1 cut(s) | 1010 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 365, 397 |
| BauI | CACGAG | 3 cut(s) | 548, 1198, 2009 |
| BbsI | GAAGAC | 1 cut(s) | 1113 |
| Bbv12I | GWGCWC | 2 cut(s) | 1010, 1644 |
| BbvCI | CCTCAGC | 2 cut(s) | 1835, 1893 |
| BccI | CCATC | 8 cut(s) | 166, 497, 667, 1108, 1508, 1730, 1840, 2210 |
| BceAI | ACGGC | 8 cut(s) | 635, 968, 1430, 1460, 1611, 1659, 1937, 2000 |
| BciVI | GTATCC | 3 cut(s) | 190, 462, 1238 |
| BcoDI | GTCTC | 6 cut(s) | 1211, 1634, 1682, 1960, 2150, 2208 |
| BfaI | CTAG | 7 cut(s) | 257, 750, 789, 806, 860, 1046, 1184 |
| BfmI | CTRYAG | 2 cut(s) | 596, 1941 |
| BfoI | RGCGCY | 1 cut(s) | 545 |
| BfuAI | ACCTGC | 1 cut(s) | 1771 |
| BfuI | GTATCC | 3 cut(s) | 190, 462, 1238 |
| BlnI | CCTAGG | 1 cut(s) | 256 |
| Bme18I | GGWCC | 6 cut(s) | 151, 273, 399, 685, 928, 1437 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1186 |
| BmeT110I | CYCGRG | 6 cut(s) | 529, 972, 1433, 1635, 1683, 1920 |
| BmgT120I | GGNCC | 9 cut(s) | 151, 273, 334, 399, 685, 928, 1437, 1443, 1551 |
| BmrI | ACTGGG | 2 cut(s) | 291, 1383 |
| BmsI | GCATC | 4 cut(s) | 603, 1111, 1150, 1686 |
| BmuI | ACTGGG | 2 cut(s) | 291, 1383 |
| BpiI | GAAGAC | 1 cut(s) | 1113 |
| BplI | GAGNNNNNCTC | 6 cut(s) | 992, 1024, 1072, 1104, 1814, 1846 |
| BpmI | CTGGAG | 2 cut(s) | 2003, 2171 |
| Bpu10I | CCTNAGC | 3 cut(s) | 488, 1835, 1893 |
| BpuEI | CTTGAG | 1 cut(s) | 1898 |
| BsaBI | GATNNNNATC | 2 cut(s) | 1746, 2220 |
| BsaHI | GRCGYC | 3 cut(s) | 1176, 1266, 1728 |
| BsaI | GGTCTC | 3 cut(s) | 1211, 2150, 2208 |
| BsaWI | WCCGGW | 4 cut(s) | 249, 905, 1404, 2189 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1208, 1238, 2015, 2045 |
| Bse118I | RCCGGY | 5 cut(s) | 249, 905, 1342, 1404, 2195 |
| Bse1I | ACTGG | 7 cut(s) | 5, 214, 286, 516, 931, 1064, 1378 |
| Bse21I | CCTNAGG | 1 cut(s) | 360 |
| Bse8I | GATNNNNATC | 2 cut(s) | 1746, 2220 |
| BseGI | GGATG | 7 cut(s) | 177, 508, 618, 1020, 1119, 1123, 2044 |
| BseJI | GATNNNNATC | 2 cut(s) | 1746, 2220 |
| BseMII | CTCAG | 7 cut(s) | 351, 1139, 1362, 1826, 1833, 1884, 2219 |
| BseNI | ACTGG | 7 cut(s) | 5, 214, 286, 516, 931, 1064, 1378 |
| BseRI | GAGGAG | 5 cut(s) | 1018, 1229, 1772, 1919, 2036 |
| BseSI | GKGCMC | 2 cut(s) | 1356, 1705 |
| BseX3I | CGGCCG | 1 cut(s) | 2109 |
| BsgI | GTGCAG | 2 cut(s) | 530, 1151 |
| Bsh1236I | CGCG | 5 cut(s) | 156, 726, 728, 1281, 1286 |
| Bsh1285I | CGRYCG | 3 cut(s) | 250, 1588, 2112 |
| BshNI | GGYRCC | 6 cut(s) | 158, 252, 591, 908, 1401, 2192 |
| BshTI | ACCGGT | 3 cut(s) | 249, 905, 1404 |
| BsiEI | CGRYCG | 3 cut(s) | 250, 1588, 2112 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1010, 1644 |
| BsiHKCI | CYCGRG | 6 cut(s) | 529, 972, 1433, 1635, 1683, 1920 |
| BslFI | GGGAC | 7 cut(s) | 412, 698, 806, 941, 1450, 1841, 1937 |
| BsmAI | GTCTC | 6 cut(s) | 1211, 1634, 1682, 1960, 2150, 2208 |
| BsmBI | CGTCTC | 3 cut(s) | 1634, 1682, 1960 |
| BsmFI | GGGAC | 7 cut(s) | 412, 698, 806, 941, 1450, 1841, 1937 |
| BsmI | GAATGC | 2 cut(s) | 366, 652 |
| Bso31I | GGTCTC | 3 cut(s) | 1211, 2150, 2208 |
| BsoBI | CYCGRG | 6 cut(s) | 529, 972, 1433, 1635, 1683, 1920 |
| Bsp1286I | GDGCHC | 4 cut(s) | 1010, 1356, 1644, 1705 |
| Bsp143I | GATC | 7 cut(s) | 203, 323, 1258, 1427, 1564, 1987, 2221 |
| Bsp19I | CCATGG | 1 cut(s) | 1296 |
| BspCNI | CTCAG | 7 cut(s) | 352, 1138, 1361, 1827, 1834, 1885, 2218 |
| BspFNI | CGCG | 5 cut(s) | 156, 726, 728, 1281, 1286 |
| BspHI | TCATGA | 1 cut(s) | 2085 |
| BspMAI | CTGCAG | 1 cut(s) | 1945 |
| BspMI | ACCTGC | 1 cut(s) | 1771 |
| BspPI | GGATC | 5 cut(s) | 211, 331, 1253, 1572, 1995 |
| BspT107I | GGYRCC | 6 cut(s) | 158, 252, 591, 908, 1401, 2192 |
| BspTNI | GGTCTC | 3 cut(s) | 1211, 2150, 2208 |
| BsrBI | CCGCTC | 1 cut(s) | 1825 |
| BsrFI | RCCGGY | 5 cut(s) | 249, 905, 1342, 1404, 2195 |
| BsrI | ACTGG | 7 cut(s) | 5, 214, 286, 516, 931, 1064, 1378 |
| BssAI | RCCGGY | 5 cut(s) | 249, 905, 1342, 1404, 2195 |
| BssMI | GATC | 7 cut(s) | 203, 323, 1258, 1427, 1564, 1987, 2221 |
| BssNI | GRCGYC | 3 cut(s) | 1176, 1266, 1728 |
| BssSI | CACGAG | 3 cut(s) | 548, 1198, 2009 |
| BssT1I | CCWWGG | 3 cut(s) | 256, 565, 1296 |
| Bst2BI | CACGAG | 3 cut(s) | 548, 1198, 2009 |
| Bst4CI | ACNGT | 5 cut(s) | 349, 600, 744, 775, 1250 |
| Bst6I | CTCTTC | 3 cut(s) | 956, 1651, 1712 |
| BstACI | GRCGYC | 3 cut(s) | 1176, 1266, 1728 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1949 |
| BstC8I | GCNNGC | 6 cut(s) | 231, 695, 1551, 1701, 1732, 2058 |
| BstDSI | CCRYGG | 3 cut(s) | 154, 1296, 2016 |
| BstEII | GGTNACC | 1 cut(s) | 775 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 223, 1092 |
| BstF5I | GGATG | 7 cut(s) | 177, 508, 618, 1020, 1119, 1123, 2044 |
| BstFNI | CGCG | 5 cut(s) | 156, 726, 728, 1281, 1286 |
| BstH2I | RGCGCY | 1 cut(s) | 545 |
| BstHHI | GCGC | 5 cut(s) | 479, 544, 728, 1283, 1288 |
| BstKTI | GATC | 7 cut(s) | 206, 326, 1261, 1430, 1567, 1990, 2224 |
| BstMAI | GTCTC | 6 cut(s) | 1211, 1634, 1682, 1960, 2150, 2208 |
| BstMBI | GATC | 7 cut(s) | 203, 323, 1258, 1427, 1564, 1987, 2221 |
| BstMCI | CGRYCG | 3 cut(s) | 250, 1588, 2112 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 230, 583, 613, 703, 1555, 1727, 1831, 1949, 2057 |
| BstNSI | RCATGY | 2 cut(s) | 454, 1141 |
| BstPI | GGTNACC | 1 cut(s) | 775 |
| BstSFI | CTRYAG | 2 cut(s) | 596, 1941 |
| BstSLI | GKGCMC | 2 cut(s) | 1356, 1705 |
| BstUI | CGCG | 5 cut(s) | 156, 726, 728, 1281, 1286 |
| BstV2I | GAAGAC | 1 cut(s) | 1113 |
| BstX2I | RGATCY | 2 cut(s) | 323, 1564 |
| BstYI | RGATCY | 2 cut(s) | 323, 1564 |
| BstZI | CGGCCG | 1 cut(s) | 2109 |
| Bsu36I | CCTNAGG | 1 cut(s) | 360 |
| BsuI | GTATCC | 3 cut(s) | 190, 462, 1238 |
| BtgI | CCRYGG | 3 cut(s) | 154, 1296, 2016 |
| BtgZI | GCGATG | 2 cut(s) | 423, 1395 |
| BtsCI | GGATG | 7 cut(s) | 177, 508, 618, 1020, 1119, 1123, 2044 |
| BtsIMutI | CAGTG | 5 cut(s) | 12, 354, 1071, 1356, 1371 |
| BveI | ACCTGC | 1 cut(s) | 1771 |
| Cac8I | GCNNGC | 6 cut(s) | 231, 695, 1551, 1701, 1732, 2058 |
| CaiI | CAGNNNCTG | 3 cut(s) | 604, 1513, 1949 |
| CciI | TCATGA | 1 cut(s) | 2085 |
| CfoI | GCGC | 5 cut(s) | 479, 544, 728, 1283, 1288 |
| Cfr10I | RCCGGY | 5 cut(s) | 249, 905, 1342, 1404, 2195 |
| Cfr13I | GGNCC | 9 cut(s) | 151, 273, 334, 399, 685, 928, 1437, 1443, 1551 |
| Cfr42I | CCGCGG | 1 cut(s) | 157 |
| Cfr9I | CCCGGG | 4 cut(s) | 529, 972, 1433, 1920 |
| CseI | GACGC | 2 cut(s) | 1274, 1736 |
| CsiI | ACCWGGT | 1 cut(s) | 735 |
| CspAI | ACCGGT | 3 cut(s) | 249, 905, 1404 |
| CviAII | CATG | 9 cut(s) | 427, 451, 679, 1138, 1297, 1555, 1796, 1959, 2086 |
| DpnI | GATC | 7 cut(s) | 205, 325, 1260, 1429, 1566, 1989, 2223 |
| DpnII | GATC | 7 cut(s) | 203, 323, 1258, 1427, 1564, 1987, 2221 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1186 |
| EaeI | YGGCCR | 7 cut(s) | 351, 513, 675, 1299, 1848, 2109, 2181 |
| EagI | CGGCCG | 1 cut(s) | 2109 |
| Eam1104I | CTCTTC | 3 cut(s) | 956, 1651, 1712 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1186 |
| EarI | CTCTTC | 3 cut(s) | 956, 1651, 1712 |
| Ecl136II | GAGCTC | 1 cut(s) | 1008 |
| EclXI | CGGCCG | 1 cut(s) | 2109 |
| Eco130I | CCWWGG | 3 cut(s) | 256, 565, 1296 |
| Eco147I | AGGCCT | 1 cut(s) | 493 |
| Eco24I | GRGCYC | 1 cut(s) | 1010 |
| Eco31I | GGTCTC | 3 cut(s) | 1211, 2150, 2208 |
| Eco47I | GGWCC | 6 cut(s) | 151, 273, 399, 685, 928, 1437 |
| Eco52I | CGGCCG | 1 cut(s) | 2109 |
| Eco53kI | GAGCTC | 1 cut(s) | 1008 |
| Eco57I | CTGAAG | 4 cut(s) | 479, 1589, 1632, 1772 |
| Eco81I | CCTNAGG | 1 cut(s) | 360 |
| Eco88I | CYCGRG | 6 cut(s) | 529, 972, 1433, 1635, 1683, 1920 |
| Eco91I | GGTNACC | 1 cut(s) | 775 |
| EcoICRI | GAGCTC | 1 cut(s) | 1008 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 223, 1092 |
| EcoO109I | RGGNCCY | 3 cut(s) | 273, 334, 685 |
| EcoO65I | GGTNACC | 1 cut(s) | 775 |
| EcoT14I | CCWWGG | 3 cut(s) | 256, 565, 1296 |
| EcoT38I | GRGCYC | 1 cut(s) | 1010 |
| ErhI | CCWWGG | 3 cut(s) | 256, 565, 1296 |
| Esp3I | CGTCTC | 3 cut(s) | 1634, 1682, 1960 |
| FaeI | CATG | 9 cut(s) | 430, 454, 682, 1141, 1300, 1558, 1799, 1962, 2089 |
| FaqI | GGGAC | 7 cut(s) | 412, 698, 806, 941, 1450, 1841, 1937 |
| FatI | CATG | 9 cut(s) | 426, 450, 678, 1137, 1296, 1554, 1795, 1958, 2085 |
| FauI | CCCGC | 6 cut(s) | 760, 1286, 1480, 1542, 1692, 1818 |
| FauNDI | CATATG | 1 cut(s) | 404 |
| FblI | GTMKAC | 2 cut(s) | 447, 1179 |
| FokI | GGATG | 7 cut(s) | 184, 515, 625, 1007, 1126, 1130, 2051 |
| FriOI | GRGCYC | 1 cut(s) | 1010 |
| FspBI | CTAG | 7 cut(s) | 257, 750, 789, 806, 860, 1046, 1184 |
| GlaI | GCGC | 5 cut(s) | 478, 543, 727, 1282, 1287 |
| GsuI | CTGGAG | 2 cut(s) | 2003, 2171 |
| HaeII | RGCGCY | 1 cut(s) | 545 |
| HgaI | GACGC | 2 cut(s) | 1274, 1736 |
| HhaI | GCGC | 5 cut(s) | 479, 544, 728, 1283, 1288 |
| Hin1I | GRCGYC | 3 cut(s) | 1176, 1266, 1728 |
| Hin1II | CATG | 9 cut(s) | 430, 454, 682, 1141, 1300, 1558, 1799, 1962, 2089 |
| Hin6I | GCGC | 5 cut(s) | 477, 542, 726, 1281, 1286 |
| HinP1I | GCGC | 5 cut(s) | 477, 542, 726, 1281, 1286 |
| HincII | GTYRAC | 1 cut(s) | 1180 |
| HindII | GTYRAC | 1 cut(s) | 1180 |
| HphI | GGTGA | 8 cut(s) | 618, 787, 1262, 1422, 1624, 1672, 1795, 1895 |
| Hpy166II | GTNNAC | 6 cut(s) | 384, 448, 733, 1180, 1253, 1717 |
| Hpy188III | TCNNGA | 6 cut(s) | 131, 359, 1262, 1912, 1982, 2086 |
| Hpy8I | GTNNAC | 6 cut(s) | 384, 448, 733, 1180, 1253, 1717 |
| Hpy99I | CGWCG | 7 cut(s) | 211, 317, 1178, 1181, 1268, 1451, 1730 |
| HpyCH4III | ACNGT | 5 cut(s) | 349, 600, 744, 775, 1250 |
| HpyCH4IV | ACGT | 1 cut(s) | 1176 |
| HpyCH4V | TGCA | 5 cut(s) | 547, 666, 1168, 1410, 1943 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 230, 583, 613, 703, 1555, 1727, 1831, 1949, 2057 |
| HpySE526I | ACGT | 1 cut(s) | 1176 |
| Hsp92I | GRCGYC | 3 cut(s) | 1176, 1266, 1728 |
| Hsp92II | CATG | 9 cut(s) | 430, 454, 682, 1141, 1300, 1558, 1799, 1962, 2089 |
| HspAI | GCGC | 5 cut(s) | 477, 542, 726, 1281, 1286 |
| KpnI | GGTACC | 4 cut(s) | 162, 256, 912, 1405 |
| KspI | CCGCGG | 1 cut(s) | 157 |
| Kzo9I | GATC | 7 cut(s) | 203, 323, 1258, 1427, 1564, 1987, 2221 |
| LmnI | GCTCC | 7 cut(s) | 564, 1005, 1377, 1398, 1822, 1873, 2167 |
| LweI | GCATC | 4 cut(s) | 603, 1111, 1150, 1686 |
| MabI | ACCWGGT | 1 cut(s) | 735 |
| MaeI | CTAG | 7 cut(s) | 257, 750, 789, 806, 860, 1046, 1184 |
| MaeII | ACGT | 1 cut(s) | 1176 |
| MaeIII | GTNAC | 6 cut(s) | 343, 624, 775, 808, 1273, 1766 |
| MalI | GATC | 7 cut(s) | 205, 325, 1260, 1429, 1566, 1989, 2223 |
| MbiI | CCGCTC | 1 cut(s) | 1825 |
| MboI | GATC | 7 cut(s) | 203, 323, 1258, 1427, 1564, 1987, 2221 |
| MboII | GAAGA | 9 cut(s) | 973, 1118, 1169, 1433, 1638, 1699, 1751, 1784, 2173 |
| MfeI | CAATTG | 1 cut(s) | 1209 |
| MflI | RGATCY | 2 cut(s) | 323, 1564 |
| MhlI | GDGCHC | 4 cut(s) | 1010, 1356, 1644, 1705 |
| MlsI | TGGCCA | 3 cut(s) | 353, 677, 1850 |
| MluCI | AATT | 5 cut(s) | 68, 88, 1209, 1541, 2132 |
| MluNI | TGGCCA | 3 cut(s) | 353, 677, 1850 |
| MlyI | GAGTC | 6 cut(s) | 1210, 1697, 1823, 1919, 1955, 1970 |
| MmeI | TCCRAC | 3 cut(s) | 242, 1206, 1794 |
| Mox20I | TGGCCA | 3 cut(s) | 353, 677, 1850 |
| MscI | TGGCCA | 3 cut(s) | 353, 677, 1850 |
| MseI | TTAA | 3 cut(s) | 66, 1130, 2094 |
| MslI | CAYNNNNRTG | 3 cut(s) | 431, 1142, 1328 |
| Msp20I | TGGCCA | 3 cut(s) | 353, 677, 1850 |
| MspA1I | CMGCKG | 4 cut(s) | 156, 901, 1510, 1864 |
| MteI | GCGCNGCGC | 1 cut(s) | 1284 |
| MunI | CAATTG | 1 cut(s) | 1209 |
| Mva1269I | GAATGC | 2 cut(s) | 366, 652 |
| MvnI | CGCG | 5 cut(s) | 156, 726, 728, 1281, 1286 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 230, 583, 613, 703, 1555, 1727, 1831, 1949, 2057 |
| NcoI | CCATGG | 1 cut(s) | 1296 |
| NdeI | CATATG | 1 cut(s) | 404 |
| NdeII | GATC | 7 cut(s) | 203, 323, 1258, 1427, 1564, 1987, 2221 |
| NlaIII | CATG | 9 cut(s) | 430, 454, 682, 1141, 1300, 1558, 1799, 1962, 2089 |
| NmeAIII | GCCGAG | 1 cut(s) | 402 |
| NmuCI | GTSAC | 3 cut(s) | 624, 775, 1273 |
| NspI | RCATGY | 2 cut(s) | 454, 1141 |
| OliI | CACNNNNGTG | 1 cut(s) | 1328 |
| PagI | TCATGA | 1 cut(s) | 2085 |
| PaqCI | CACCTGC | 1 cut(s) | 1771 |
| PasI | CCCWGGG | 1 cut(s) | 1397 |
| PceI | AGGCCT | 1 cut(s) | 493 |
| PcsI | WCGNNNNNNNCGW | 5 cut(s) | 206, 275, 1196, 1530, 1731 |
| PctI | GAATGC | 2 cut(s) | 366, 652 |
| PfeI | GAWTC | 5 cut(s) | 176, 553, 1495, 1621, 1669 |
| PfoI | TCCNGGA | 1 cut(s) | 2148 |
| PinAI | ACCGGT | 3 cut(s) | 249, 905, 1404 |
| PleI | GAGTC | 6 cut(s) | 1209, 1696, 1823, 1919, 1954, 1970 |
| PpsI | GAGTC | 6 cut(s) | 1209, 1696, 1823, 1919, 1954, 1970 |
| PpuMI | RGGWCCY | 2 cut(s) | 273, 685 |
| Psp124BI | GAGCTC | 1 cut(s) | 1010 |
| Psp5II | RGGWCCY | 2 cut(s) | 273, 685 |
| PspEI | GGTNACC | 1 cut(s) | 775 |
| PspPI | GGNCC | 9 cut(s) | 151, 273, 334, 399, 685, 928, 1437, 1443, 1551 |
| PspPPI | RGGWCCY | 2 cut(s) | 273, 685 |
| PstI | CTGCAG | 1 cut(s) | 1945 |
| PstNI | CAGNNNCTG | 3 cut(s) | 604, 1513, 1949 |
| PsuI | RGATCY | 2 cut(s) | 323, 1564 |
| PvuII | CAGCTG | 2 cut(s) | 901, 1510 |
| RseI | CAYNNNNRTG | 3 cut(s) | 431, 1142, 1328 |
| SacI | GAGCTC | 1 cut(s) | 1010 |
| SacII | CCGCGG | 1 cut(s) | 157 |
| SalI | GTCGAC | 1 cut(s) | 1178 |
| SaqAI | TTAA | 3 cut(s) | 66, 1130, 2094 |
| Sau3AI | GATC | 7 cut(s) | 203, 323, 1258, 1427, 1564, 1987, 2221 |
| Sau96I | GGNCC | 9 cut(s) | 151, 273, 334, 399, 685, 928, 1437, 1443, 1551 |
| SchI | GAGTC | 6 cut(s) | 1210, 1697, 1823, 1919, 1955, 1970 |
| SduI | GDGCHC | 4 cut(s) | 1010, 1356, 1644, 1705 |
| SexAI | ACCWGGT | 1 cut(s) | 735 |
| SfaNI | GCATC | 4 cut(s) | 603, 1111, 1150, 1686 |
| SfcI | CTRYAG | 2 cut(s) | 596, 1941 |
| Sfr303I | CCGCGG | 1 cut(s) | 157 |
| SgrBI | CCGCGG | 1 cut(s) | 157 |
| SinI | GGWCC | 6 cut(s) | 151, 273, 399, 685, 928, 1437 |
| SmaI | CCCGGG | 4 cut(s) | 531, 974, 1435, 1922 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 431, 1142, 1328 |
| SmlI | CTYRAG | 1 cut(s) | 1877 |
| SmoI | CTYRAG | 1 cut(s) | 1877 |
| Sse9I | AATT | 5 cut(s) | 68, 88, 1209, 1541, 2132 |
| SseBI | AGGCCT | 1 cut(s) | 493 |
| SspMI | CTAG | 7 cut(s) | 257, 750, 789, 806, 860, 1046, 1184 |
| SstI | GAGCTC | 1 cut(s) | 1010 |
| StuI | AGGCCT | 1 cut(s) | 493 |
| StyI | CCWWGG | 3 cut(s) | 256, 565, 1296 |
| TaaI | ACNGT | 5 cut(s) | 349, 600, 744, 775, 1250 |
| TaiI | ACGT | 1 cut(s) | 1179 |
| TaqII | GACCGA | 1 cut(s) | 1194 |
| TasI | AATT | 5 cut(s) | 68, 88, 1209, 1541, 2132 |
| TatI | WGTACW | 1 cut(s) | 2025 |
| TauI | GCSGC | 8 cut(s) | 457, 518, 788, 805, 1286, 2111, 2180, 2183 |
| TfiI | GAWTC | 5 cut(s) | 176, 553, 1495, 1621, 1669 |
| Tru1I | TTAA | 3 cut(s) | 66, 1130, 2094 |
| Tru9I | TTAA | 3 cut(s) | 66, 1130, 2094 |
| TscAI | CASTG | 5 cut(s) | 12, 354, 1071, 1356, 1378 |
| TseFI | GTSAC | 3 cut(s) | 624, 775, 1273 |
| Tsp45I | GTSAC | 3 cut(s) | 624, 775, 1273 |
| TspDTI | ATGAA | 8 cut(s) | 426, 449, 1009, 1533, 1551, 1784, 2102, 2139 |
| TspMI | CCCGGG | 4 cut(s) | 529, 972, 1433, 1920 |
| TspRI | CASTG | 5 cut(s) | 12, 354, 1071, 1356, 1378 |
| VpaK11BI | GGWCC | 6 cut(s) | 151, 273, 399, 685, 928, 1437 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 223, 1092 |
| XapI | RAATTY | 4 cut(s) | 68, 88, 1541, 2132 |
| XceI | RCATGY | 2 cut(s) | 454, 1141 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 169 |
| XmaI | CCCGGG | 4 cut(s) | 529, 972, 1433, 1920 |
| XmaJI | CCTAGG | 1 cut(s) | 256 |
| XmiI | GTMKAC | 2 cut(s) | 447, 1179 |
| XspI | CTAG | 7 cut(s) | 257, 750, 789, 806, 860, 1046, 1184 |
| ZraI | GACGTC | 1 cut(s) | 1177 |