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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 242 |
| AciI | CCGC | 3 cut(s) | 188, 235, 251 |
| AclWI | GGATC | 4 cut(s) | 40, 114, 127, 190 |
| AcoI | YGGCCR | 1 cut(s) | 210 |
| AcuI | CTGAAG | 1 cut(s) | 84 |
| AfaI | GTAC | 2 cut(s) | 41, 83 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 129 |
| AjnI | CCWGG | 1 cut(s) | 53 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 104, 136 |
| AluBI | AGCT | 1 cut(s) | 62 |
| AluI | AGCT | 1 cut(s) | 62 |
| Alw26I | GTCTC | 1 cut(s) | 307 |
| AlwI | GGATC | 4 cut(s) | 40, 114, 127, 190 |
| Ama87I | CYCGRG | 2 cut(s) | 218, 275 |
| AoxI | GGCC | 3 cut(s) | 88, 192, 210 |
| AspS9I | GGNCC | 2 cut(s) | 193, 258 |
| AsuC2I | CCSGG | 3 cut(s) | 197, 276, 277 |
| AsuHPI | GGTGA | 1 cut(s) | 179 |
| AvaI | CYCGRG | 2 cut(s) | 218, 275 |
| AvaII | GGWCC | 1 cut(s) | 258 |
| BalI | TGGCCA | 1 cut(s) | 212 |
| BamHI | GGATCC | 1 cut(s) | 119 |
| BbsI | GAAGAC | 1 cut(s) | 39 |
| BccI | CCATC | 1 cut(s) | 248 |
| BciT130I | CCWGG | 1 cut(s) | 55 |
| BciVI | GTATCC | 1 cut(s) | 19 |
| BcnI | CCSGG | 3 cut(s) | 197, 276, 277 |
| BcoDI | GTCTC | 1 cut(s) | 307 |
| BfuI | GTATCC | 1 cut(s) | 19 |
| BmcAI | AGTACT | 1 cut(s) | 83 |
| Bme1390I | CCNGG | 4 cut(s) | 55, 197, 276, 277 |
| Bme18I | GGWCC | 1 cut(s) | 258 |
| BmeT110I | CYCGRG | 2 cut(s) | 218, 275 |
| BmgT120I | GGNCC | 2 cut(s) | 193, 258 |
| BmiI | GGNNCC | 3 cut(s) | 121, 195, 259 |
| BmrFI | CCNGG | 4 cut(s) | 55, 197, 276, 277 |
| BmrI | ACTGGG | 1 cut(s) | 138 |
| BmuI | ACTGGG | 1 cut(s) | 138 |
| BpiI | GAAGAC | 1 cut(s) | 39 |
| BpuMI | CCSGG | 3 cut(s) | 197, 276, 277 |
| BsaJI | CCNNGG | 3 cut(s) | 195, 219, 275 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 129 |
| Bse1I | ACTGG | 3 cut(s) | 43, 79, 133 |
| BseBI | CCWGG | 1 cut(s) | 55 |
| BseDI | CCNNGG | 3 cut(s) | 195, 219, 275 |
| BseGI | GGATG | 1 cut(s) | 259 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 129 |
| BseNI | ACTGG | 3 cut(s) | 43, 79, 133 |
| BseRI | GAGGAG | 1 cut(s) | 167 |
| BshFI | GGCC | 3 cut(s) | 90, 194, 212 |
| BsiHKCI | CYCGRG | 2 cut(s) | 218, 275 |
| BsiSI | CCGG | 2 cut(s) | 197, 276 |
| BslFI | GGGAC | 1 cut(s) | 271 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 129 |
| BsmAI | GTCTC | 1 cut(s) | 307 |
| BsmFI | GGGAC | 1 cut(s) | 271 |
| BsnI | GGCC | 3 cut(s) | 90, 194, 212 |
| BsoBI | CYCGRG | 2 cut(s) | 218, 275 |
| Bsp143I | GATC | 5 cut(s) | 32, 97, 119, 182, 300 |
| BspACI | CCGC | 3 cut(s) | 188, 235, 251 |
| BspANI | GGCC | 3 cut(s) | 90, 194, 212 |
| BspLI | GGNNCC | 3 cut(s) | 121, 195, 259 |
| BspPI | GGATC | 4 cut(s) | 40, 114, 127, 190 |
| BsrI | ACTGG | 3 cut(s) | 43, 79, 133 |
| BssECI | CCNNGG | 3 cut(s) | 195, 219, 275 |
| BssMI | GATC | 5 cut(s) | 32, 97, 119, 182, 300 |
| BssNAI | GTATAC | 1 cut(s) | 243 |
| Bst1107I | GTATAC | 1 cut(s) | 243 |
| Bst2UI | CCWGG | 1 cut(s) | 55 |
| Bst4CI | ACNGT | 2 cut(s) | 208, 262 |
| BstC8I | GCNNGC | 1 cut(s) | 60 |
| BstDEI | CTNAG | 1 cut(s) | 85 |
| BstF5I | GGATG | 1 cut(s) | 259 |
| BstKTI | GATC | 5 cut(s) | 35, 100, 122, 185, 303 |
| BstMAI | GTCTC | 1 cut(s) | 307 |
| BstMBI | GATC | 5 cut(s) | 32, 97, 119, 182, 300 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 59 |
| BstNI | CCWGG | 1 cut(s) | 55 |
| BstSCI | CCNGG | 4 cut(s) | 53, 195, 274, 275 |
| BstV2I | GAAGAC | 1 cut(s) | 39 |
| BstX2I | RGATCY | 2 cut(s) | 119, 182 |
| BstYI | RGATCY | 2 cut(s) | 119, 182 |
| BstZ17I | GTATAC | 1 cut(s) | 243 |
| BsuI | GTATCC | 1 cut(s) | 19 |
| BsuRI | GGCC | 3 cut(s) | 90, 194, 212 |
| BtsCI | GGATG | 1 cut(s) | 259 |
| BtsIMutI | CAGTG | 1 cut(s) | 213 |
| Cac8I | GCNNGC | 1 cut(s) | 60 |
| Cfr13I | GGNCC | 2 cut(s) | 193, 258 |
| Cfr9I | CCCGGG | 1 cut(s) | 275 |
| Csp6I | GTAC | 2 cut(s) | 40, 82 |
| CviAII | CATG | 1 cut(s) | 310 |
| CviJI | RGCY | 4 cut(s) | 62, 90, 194, 212 |
| CviKI_1 | RGCY | 4 cut(s) | 62, 90, 194, 212 |
| CviQI | GTAC | 2 cut(s) | 40, 82 |
| DdeI | CTNAG | 1 cut(s) | 85 |
| DpnI | GATC | 5 cut(s) | 34, 99, 121, 184, 302 |
| DpnII | GATC | 5 cut(s) | 32, 97, 119, 182, 300 |
| EaeI | YGGCCR | 1 cut(s) | 210 |
| Eco32I | GATATC | 1 cut(s) | 73 |
| Eco47I | GGWCC | 1 cut(s) | 258 |
| Eco57I | CTGAAG | 1 cut(s) | 84 |
| Eco88I | CYCGRG | 2 cut(s) | 218, 275 |
| EcoO109I | RGGNCCY | 1 cut(s) | 193 |
| EcoRII | CCWGG | 1 cut(s) | 53 |
| EcoRV | GATATC | 1 cut(s) | 73 |
| FaeI | CATG | 1 cut(s) | 313 |
| FaiI | YATR | 5 cut(s) | 227, 243, 265, 287, 311 |
| FaqI | GGGAC | 1 cut(s) | 271 |
| FatI | CATG | 1 cut(s) | 309 |
| FblI | GTMKAC | 1 cut(s) | 242 |
| FokI | GGATG | 1 cut(s) | 266 |
| HaeIII | GGCC | 3 cut(s) | 90, 194, 212 |
| HapII | CCGG | 2 cut(s) | 197, 276 |
| Hin1II | CATG | 1 cut(s) | 313 |
| HinfI | GANTC | 2 cut(s) | 5, 155 |
| HpaII | CCGG | 2 cut(s) | 197, 276 |
| HphI | GGTGA | 1 cut(s) | 179 |
| Hpy166II | GTNNAC | 1 cut(s) | 243 |
| Hpy188I | TCNGA | 3 cut(s) | 10, 142, 160 |
| Hpy188III | TCNNGA | 1 cut(s) | 218 |
| Hpy8I | GTNNAC | 1 cut(s) | 243 |
| Hpy99I | CGWCG | 3 cut(s) | 40, 164, 272 |
| HpyAV | CCTTC | 2 cut(s) | 5, 289 |
| HpyCH4III | ACNGT | 2 cut(s) | 208, 262 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 59 |
| HpyF3I | CTNAG | 1 cut(s) | 85 |
| Hsp92II | CATG | 1 cut(s) | 313 |
| Kzo9I | GATC | 5 cut(s) | 32, 97, 119, 182, 300 |
| LpnPI | CCDG | 7 cut(s) | 40, 56, 67, 92, 114, 210, 289 |
| MaeIII | GTNAC | 1 cut(s) | 202 |
| MalI | GATC | 5 cut(s) | 34, 99, 121, 184, 302 |
| MboI | GATC | 5 cut(s) | 32, 97, 119, 182, 300 |
| MboII | GAAGA | 1 cut(s) | 39 |
| MflI | RGATCY | 2 cut(s) | 119, 182 |
| MlsI | TGGCCA | 1 cut(s) | 212 |
| MluCI | AATT | 1 cut(s) | 101 |
| MluNI | TGGCCA | 1 cut(s) | 212 |
| MlyI | GAGTC | 1 cut(s) | 164 |
| MmeI | TCCRAC | 1 cut(s) | 183 |
| MnlI | CCTC | 6 cut(s) | 133, 145, 157, 169, 184, 214 |
| Mox20I | TGGCCA | 1 cut(s) | 212 |
| MscI | TGGCCA | 1 cut(s) | 212 |
| MslI | CAYNNNNRTG | 1 cut(s) | 308 |
| Msp20I | TGGCCA | 1 cut(s) | 212 |
| MspI | CCGG | 2 cut(s) | 197, 276 |
| MspR9I | CCNGG | 4 cut(s) | 55, 197, 276, 277 |
| MvaI | CCWGG | 1 cut(s) | 55 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 59 |
| NciI | CCSGG | 3 cut(s) | 197, 276, 277 |
| NdeII | GATC | 5 cut(s) | 32, 97, 119, 182, 300 |
| NlaIII | CATG | 1 cut(s) | 313 |
| NlaIV | GGNNCC | 3 cut(s) | 121, 195, 259 |
| NmeAIII | GCCGAG | 1 cut(s) | 116 |
| NmuCI | GTSAC | 1 cut(s) | 202 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 35, 122 |
| PfeI | GAWTC | 1 cut(s) | 5 |
| PleI | GAGTC | 1 cut(s) | 163 |
| PpsI | GAGTC | 1 cut(s) | 163 |
| Psp6I | CCWGG | 1 cut(s) | 53 |
| PspGI | CCWGG | 1 cut(s) | 53 |
| PspN4I | GGNNCC | 3 cut(s) | 121, 195, 259 |
| PspPI | GGNCC | 2 cut(s) | 193, 258 |
| PsuI | RGATCY | 2 cut(s) | 119, 182 |
| RsaI | GTAC | 2 cut(s) | 41, 83 |
| RsaNI | GTAC | 2 cut(s) | 40, 82 |
| RseI | CAYNNNNRTG | 1 cut(s) | 308 |
| Sau3AI | GATC | 5 cut(s) | 32, 97, 119, 182, 300 |
| Sau96I | GGNCC | 2 cut(s) | 193, 258 |
| ScaI | AGTACT | 1 cut(s) | 83 |
| SchI | GAGTC | 1 cut(s) | 164 |
| ScrFI | CCNGG | 4 cut(s) | 55, 197, 276, 277 |
| SetI | ASST | 1 cut(s) | 64 |
| SinI | GGWCC | 1 cut(s) | 258 |
| SmaI | CCCGGG | 1 cut(s) | 277 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 308 |
| Sse9I | AATT | 1 cut(s) | 101 |
| SsiI | CCGC | 3 cut(s) | 188, 235, 251 |
| StyD4I | CCNGG | 4 cut(s) | 53, 195, 274, 275 |
| TaaI | ACNGT | 2 cut(s) | 208, 262 |
| TaqI | TCGA | 2 cut(s) | 125, 306 |
| TasI | AATT | 1 cut(s) | 101 |
| TatI | WGTACW | 1 cut(s) | 81 |
| TfiI | GAWTC | 1 cut(s) | 5 |
| TscAI | CASTG | 1 cut(s) | 213 |
| TseFI | GTSAC | 1 cut(s) | 202 |
| Tsp45I | GTSAC | 1 cut(s) | 202 |
| TspDTI | ATGAA | 1 cut(s) | 308 |
| TspMI | CCCGGG | 1 cut(s) | 275 |
| TspRI | CASTG | 1 cut(s) | 213 |
| VpaK11BI | GGWCC | 1 cut(s) | 258 |
| XmaI | CCCGGG | 1 cut(s) | 275 |
| XmiI | GTMKAC | 1 cut(s) | 242 |
| ZrmI | AGTACT | 1 cut(s) | 83 |