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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 142 |
| AccB1I | GGYRCC | 1 cut(s) | 142 |
| AccII | CGCG | 2 cut(s) | 29, 69 |
| AciI | CCGC | 4 cut(s) | 27, 38, 69, 362 |
| AclWI | GGATC | 2 cut(s) | 16, 113 |
| AfaI | GTAC | 3 cut(s) | 76, 144, 323 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 377 |
| AgeI | ACCGGT | 1 cut(s) | 54 |
| AjiI | CACGTC | 1 cut(s) | 244 |
| AleI | CACNNNNGTG | 1 cut(s) | 236 |
| AlwI | GGATC | 2 cut(s) | 16, 113 |
| Ama87I | CYCGRG | 3 cut(s) | 125, 206, 263 |
| AoxI | GGCC | 2 cut(s) | 338, 348 |
| AsiGI | ACCGGT | 1 cut(s) | 54 |
| Asp718I | GGTACC | 1 cut(s) | 142 |
| AspLEI | GCGC | 1 cut(s) | 184 |
| AsuC2I | CCSGG | 3 cut(s) | 264, 265, 352 |
| AvaI | CYCGRG | 3 cut(s) | 125, 206, 263 |
| BanI | GGYRCC | 1 cut(s) | 142 |
| BanII | GRGCYC | 1 cut(s) | 264 |
| BccI | CCATC | 3 cut(s) | 142, 352, 375 |
| BcnI | CCSGG | 3 cut(s) | 264, 265, 352 |
| BfaI | CTAG | 4 cut(s) | 174, 225, 236, 397 |
| BisI | GCNGC | 1 cut(s) | 363 |
| BlsI | GCNGC | 1 cut(s) | 364 |
| Bme1390I | CCNGG | 3 cut(s) | 264, 265, 352 |
| BmeT110I | CYCGRG | 3 cut(s) | 125, 206, 263 |
| BmgBI | CACGTC | 1 cut(s) | 244 |
| BmiI | GGNNCC | 1 cut(s) | 144 |
| BmrFI | CCNGG | 3 cut(s) | 264, 265, 352 |
| BpuMI | CCSGG | 3 cut(s) | 264, 265, 352 |
| BsaJI | CCNNGG | 3 cut(s) | 207, 263, 351 |
| BsaWI | WCCGGW | 2 cut(s) | 54, 102 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 377 |
| Bse118I | RCCGGY | 1 cut(s) | 54 |
| BseDI | CCNNGG | 3 cut(s) | 207, 263, 351 |
| BseGI | GGATG | 1 cut(s) | 386 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 377 |
| Bsh1236I | CGCG | 2 cut(s) | 29, 69 |
| BshFI | GGCC | 2 cut(s) | 340, 350 |
| BshNI | GGYRCC | 1 cut(s) | 142 |
| BshTI | ACCGGT | 1 cut(s) | 54 |
| BsiHKCI | CYCGRG | 3 cut(s) | 125, 206, 263 |
| BsiSI | CCGG | 4 cut(s) | 55, 103, 264, 351 |
| BsiWI | CGTACG | 1 cut(s) | 321 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 377 |
| BsnI | GGCC | 2 cut(s) | 340, 350 |
| BsoBI | CYCGRG | 3 cut(s) | 125, 206, 263 |
| Bsp1286I | GDGCHC | 1 cut(s) | 264 |
| Bsp143I | GATC | 3 cut(s) | 8, 105, 288 |
| BspACI | CCGC | 4 cut(s) | 27, 38, 69, 362 |
| BspANI | GGCC | 2 cut(s) | 340, 350 |
| BspFNI | CGCG | 2 cut(s) | 29, 69 |
| BspLI | GGNNCC | 1 cut(s) | 144 |
| BspPI | GGATC | 2 cut(s) | 16, 113 |
| BspT107I | GGYRCC | 1 cut(s) | 142 |
| BsrFI | RCCGGY | 1 cut(s) | 54 |
| BssAI | RCCGGY | 1 cut(s) | 54 |
| BssECI | CCNNGG | 3 cut(s) | 207, 263, 351 |
| BssMI | GATC | 3 cut(s) | 8, 105, 288 |
| Bst4CI | ACNGT | 3 cut(s) | 196, 250, 321 |
| BstC8I | GCNNGC | 2 cut(s) | 36, 67 |
| BstDEI | CTNAG | 1 cut(s) | 335 |
| BstF5I | GGATG | 1 cut(s) | 386 |
| BstFNI | CGCG | 2 cut(s) | 29, 69 |
| BstHHI | GCGC | 1 cut(s) | 184 |
| BstKTI | GATC | 3 cut(s) | 11, 108, 291 |
| BstMBI | GATC | 3 cut(s) | 8, 105, 288 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 35 |
| BstSCI | CCNGG | 3 cut(s) | 262, 263, 350 |
| BstUI | CGCG | 2 cut(s) | 29, 69 |
| BstX2I | RGATCY | 1 cut(s) | 105 |
| BstYI | RGATCY | 1 cut(s) | 105 |
| BsuRI | GGCC | 2 cut(s) | 340, 350 |
| BtrI | CACGTC | 1 cut(s) | 244 |
| BtsCI | GGATG | 1 cut(s) | 386 |
| Cac8I | GCNNGC | 2 cut(s) | 36, 67 |
| CfoI | GCGC | 1 cut(s) | 184 |
| Cfr10I | RCCGGY | 1 cut(s) | 54 |
| Cfr9I | CCCGGG | 1 cut(s) | 263 |
| Csp6I | GTAC | 3 cut(s) | 75, 143, 322 |
| CspAI | ACCGGT | 1 cut(s) | 54 |
| CviAII | CATG | 1 cut(s) | 280 |
| CviJI | RGCY | 4 cut(s) | 200, 262, 340, 350 |
| CviKI_1 | RGCY | 4 cut(s) | 200, 262, 340, 350 |
| CviQI | GTAC | 3 cut(s) | 75, 143, 322 |
| DdeI | CTNAG | 1 cut(s) | 335 |
| DpnI | GATC | 3 cut(s) | 10, 107, 290 |
| DpnII | GATC | 3 cut(s) | 8, 105, 288 |
| Eco147I | AGGCCT | 1 cut(s) | 340 |
| Eco24I | GRGCYC | 1 cut(s) | 264 |
| Eco88I | CYCGRG | 3 cut(s) | 125, 206, 263 |
| EcoT38I | GRGCYC | 1 cut(s) | 264 |
| FaeI | CATG | 1 cut(s) | 283 |
| FaiI | YATR | 4 cut(s) | 83, 215, 275, 281 |
| FatI | CATG | 1 cut(s) | 279 |
| Fnu4HI | GCNGC | 1 cut(s) | 363 |
| FokI | GGATG | 1 cut(s) | 393 |
| FriOI | GRGCYC | 1 cut(s) | 264 |
| Fsp4HI | GCNGC | 1 cut(s) | 363 |
| FspBI | CTAG | 4 cut(s) | 174, 225, 236, 397 |
| GlaI | GCGC | 1 cut(s) | 183 |
| GluI | GCNGC | 1 cut(s) | 363 |
| HaeIII | GGCC | 2 cut(s) | 340, 350 |
| HapII | CCGG | 4 cut(s) | 55, 103, 264, 351 |
| HhaI | GCGC | 1 cut(s) | 184 |
| Hin1II | CATG | 1 cut(s) | 283 |
| Hin6I | GCGC | 1 cut(s) | 182 |
| HinP1I | GCGC | 1 cut(s) | 182 |
| HinfI | GANTC | 2 cut(s) | 170, 367 |
| HpaII | CCGG | 4 cut(s) | 55, 103, 264, 351 |
| Hpy166II | GTNNAC | 1 cut(s) | 241 |
| Hpy188III | TCNNGA | 4 cut(s) | 125, 127, 206, 378 |
| Hpy8I | GTNNAC | 1 cut(s) | 241 |
| Hpy99I | CGWCG | 2 cut(s) | 16, 260 |
| HpyAV | CCTTC | 2 cut(s) | 277, 384 |
| HpyCH4III | ACNGT | 3 cut(s) | 196, 250, 321 |
| HpyCH4IV | ACGT | 1 cut(s) | 243 |
| HpyCH4V | TGCA | 1 cut(s) | 394 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 35 |
| HpyF3I | CTNAG | 1 cut(s) | 335 |
| HpySE526I | ACGT | 1 cut(s) | 243 |
| Hsp92II | CATG | 1 cut(s) | 283 |
| HspAI | GCGC | 1 cut(s) | 182 |
| KpnI | GGTACC | 1 cut(s) | 146 |
| Kzo9I | GATC | 3 cut(s) | 8, 105, 288 |
| LpnPI | CCDG | 4 cut(s) | 68, 116, 277, 364 |
| MaeI | CTAG | 4 cut(s) | 174, 225, 236, 397 |
| MaeII | ACGT | 1 cut(s) | 243 |
| MaeIII | GTNAC | 2 cut(s) | 190, 244 |
| MalI | GATC | 3 cut(s) | 10, 107, 290 |
| MboI | GATC | 3 cut(s) | 8, 105, 288 |
| MboII | GAAGA | 2 cut(s) | 15, 100 |
| MflI | RGATCY | 1 cut(s) | 105 |
| MhlI | GDGCHC | 1 cut(s) | 264 |
| MnlI | CCTC | 8 cut(s) | 24, 132, 157, 172, 202, 319, 351, 381 |
| MslI | CAYNNNNRTG | 2 cut(s) | 236, 296 |
| MspI | CCGG | 4 cut(s) | 55, 103, 264, 351 |
| MspR9I | CCNGG | 3 cut(s) | 264, 265, 352 |
| MvnI | CGCG | 2 cut(s) | 29, 69 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 35 |
| NciI | CCSGG | 3 cut(s) | 264, 265, 352 |
| NdeII | GATC | 3 cut(s) | 8, 105, 288 |
| NlaIII | CATG | 1 cut(s) | 283 |
| NlaIV | GGNNCC | 1 cut(s) | 144 |
| NmuCI | GTSAC | 2 cut(s) | 190, 244 |
| OliI | CACNNNNGTG | 1 cut(s) | 236 |
| PaeR7I | CTCGAG | 1 cut(s) | 125 |
| PceI | AGGCCT | 1 cut(s) | 340 |
| PfeI | GAWTC | 2 cut(s) | 170, 367 |
| Pfl23II | CGTACG | 1 cut(s) | 321 |
| PinAI | ACCGGT | 1 cut(s) | 54 |
| PkrI | GCNGC | 1 cut(s) | 364 |
| PspLI | CGTACG | 1 cut(s) | 321 |
| PspN4I | GGNNCC | 1 cut(s) | 144 |
| PsuI | RGATCY | 1 cut(s) | 105 |
| RsaI | GTAC | 3 cut(s) | 76, 144, 323 |
| RsaNI | GTAC | 3 cut(s) | 75, 143, 322 |
| RseI | CAYNNNNRTG | 2 cut(s) | 236, 296 |
| SatI | GCNGC | 1 cut(s) | 363 |
| Sau3AI | GATC | 3 cut(s) | 8, 105, 288 |
| ScrFI | CCNGG | 3 cut(s) | 264, 265, 352 |
| SduI | GDGCHC | 1 cut(s) | 264 |
| SetI | ASST | 5 cut(s) | 213, 220, 226, 246, 330 |
| Sfr274I | CTCGAG | 1 cut(s) | 125 |
| SlaI | CTCGAG | 1 cut(s) | 125 |
| SmaI | CCCGGG | 1 cut(s) | 265 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 236, 296 |
| SmlI | CTYRAG | 1 cut(s) | 125 |
| SmoI | CTYRAG | 1 cut(s) | 125 |
| SseBI | AGGCCT | 1 cut(s) | 340 |
| SsiI | CCGC | 4 cut(s) | 27, 38, 69, 362 |
| SspMI | CTAG | 4 cut(s) | 174, 225, 236, 397 |
| StuI | AGGCCT | 1 cut(s) | 340 |
| StyD4I | CCNGG | 3 cut(s) | 262, 263, 350 |
| TaaI | ACNGT | 3 cut(s) | 196, 250, 321 |
| TaiI | ACGT | 1 cut(s) | 246 |
| TaqI | TCGA | 2 cut(s) | 111, 126 |
| TauI | GCSGC | 1 cut(s) | 365 |
| TfiI | GAWTC | 2 cut(s) | 170, 367 |
| TseFI | GTSAC | 2 cut(s) | 190, 244 |
| Tsp45I | GTSAC | 2 cut(s) | 190, 244 |
| TspDTI | ATGAA | 3 cut(s) | 170, 296, 320 |
| TspMI | CCCGGG | 1 cut(s) | 263 |
| XhoI | CTCGAG | 1 cut(s) | 125 |
| XmaI | CCCGGG | 1 cut(s) | 263 |
| XspI | CTAG | 4 cut(s) | 174, 225, 236, 397 |