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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccIII | TCCGGA | 1 cut(s) | 70 |
| AciI | CCGC | 2 cut(s) | 81, 318 |
| AclWI | GGATC | 2 cut(s) | 68, 81 |
| AcvI | CACGTG | 1 cut(s) | 66 |
| AfaI | GTAC | 2 cut(s) | 33, 43 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 77, 260, 323, 324 |
| AflIII | ACRYGT | 3 cut(s) | 63, 65, 85 |
| AjnI | CCWGG | 3 cut(s) | 76, 247, 412 |
| AluBI | AGCT | 1 cut(s) | 216 |
| AluI | AGCT | 1 cut(s) | 216 |
| Alw26I | GTCTC | 1 cut(s) | 299 |
| AlwI | GGATC | 2 cut(s) | 68, 81 |
| Ama87I | CYCGRG | 1 cut(s) | 399 |
| Aor13HI | TCCGGA | 1 cut(s) | 70 |
| AoxI | GGCC | 1 cut(s) | 341 |
| ApeKI | GCWGC | 2 cut(s) | 108, 216 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 446 |
| AspS9I | GGNCC | 3 cut(s) | 251, 311, 341 |
| AsuHPI | GGTGA | 2 cut(s) | 31, 314 |
| AsuNHI | GCTAGC | 1 cut(s) | 26 |
| AvaI | CYCGRG | 1 cut(s) | 399 |
| AvaII | GGWCC | 2 cut(s) | 251, 311 |
| BamHI | GGATCC | 1 cut(s) | 73 |
| BbrPI | CACGTG | 1 cut(s) | 66 |
| BbvI | GCAGC | 2 cut(s) | 95, 203 |
| BceAI | ACGGC | 1 cut(s) | 39 |
| BciT130I | CCWGG | 3 cut(s) | 78, 249, 414 |
| BcoDI | GTCTC | 1 cut(s) | 299 |
| BfaI | CTAG | 3 cut(s) | 27, 122, 285 |
| BisI | GCNGC | 2 cut(s) | 109, 217 |
| BlsI | GCNGC | 2 cut(s) | 110, 218 |
| Bme1390I | CCNGG | 3 cut(s) | 78, 249, 414 |
| Bme18I | GGWCC | 2 cut(s) | 251, 311 |
| BmeT110I | CYCGRG | 1 cut(s) | 399 |
| BmgT120I | GGNCC | 3 cut(s) | 251, 311, 341 |
| BmiI | GGNNCC | 1 cut(s) | 75 |
| BmrFI | CCNGG | 3 cut(s) | 78, 249, 414 |
| BmtI | GCTAGC | 1 cut(s) | 30 |
| BoxI | GACNNNNGTC | 1 cut(s) | 66 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 365, 397 |
| BsaAI | YACGTR | 1 cut(s) | 66 |
| BsaBI | GATNNNNATC | 1 cut(s) | 278 |
| BsaJI | CCNNGG | 1 cut(s) | 15 |
| BsaWI | WCCGGW | 1 cut(s) | 70 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 298, 328 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 77, 260, 323, 324 |
| Bse1I | ACTGG | 2 cut(s) | 227, 313 |
| Bse3DI | GCAATG | 3 cut(s) | 95, 118, 363 |
| Bse8I | GATNNNNATC | 1 cut(s) | 278 |
| BseAI | TCCGGA | 1 cut(s) | 70 |
| BseBI | CCWGG | 3 cut(s) | 78, 249, 414 |
| BseDI | CCNNGG | 1 cut(s) | 15 |
| BseJI | GATNNNNATC | 1 cut(s) | 278 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 77, 260, 323, 324 |
| BseMI | GCAATG | 3 cut(s) | 95, 118, 363 |
| BseNI | ACTGG | 2 cut(s) | 227, 313 |
| BseXI | GCAGC | 2 cut(s) | 95, 203 |
| BshFI | GGCC | 1 cut(s) | 343 |
| BsiHKCI | CYCGRG | 1 cut(s) | 399 |
| BsiSI | CCGG | 1 cut(s) | 71 |
| BslFI | GGGAC | 1 cut(s) | 409 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 77, 260, 323, 324 |
| BsmAI | GTCTC | 1 cut(s) | 299 |
| BsmFI | GGGAC | 1 cut(s) | 409 |
| BsnI | GGCC | 1 cut(s) | 343 |
| BsoBI | CYCGRG | 1 cut(s) | 399 |
| Bsp13I | TCCGGA | 1 cut(s) | 70 |
| Bsp1407I | TGTACA | 1 cut(s) | 41 |
| Bsp143I | GATC | 1 cut(s) | 73 |
| BspACI | CCGC | 2 cut(s) | 81, 318 |
| BspANI | GGCC | 1 cut(s) | 343 |
| BspEI | TCCGGA | 1 cut(s) | 70 |
| BspLI | GGNNCC | 1 cut(s) | 75 |
| BspOI | GCTAGC | 1 cut(s) | 30 |
| BspPI | GGATC | 2 cut(s) | 68, 81 |
| BsrDI | GCAATG | 3 cut(s) | 95, 118, 363 |
| BsrGI | TGTACA | 1 cut(s) | 41 |
| BsrI | ACTGG | 2 cut(s) | 227, 313 |
| BssECI | CCNNGG | 1 cut(s) | 15 |
| BssMI | GATC | 1 cut(s) | 73 |
| Bst2UI | CCWGG | 3 cut(s) | 78, 249, 414 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 362 |
| BstAUI | TGTACA | 1 cut(s) | 41 |
| BstBAI | YACGTR | 1 cut(s) | 66 |
| BstC8I | GCNNGC | 1 cut(s) | 28 |
| BstKTI | GATC | 1 cut(s) | 76 |
| BstMAI | GTCTC | 1 cut(s) | 299 |
| BstMBI | GATC | 1 cut(s) | 73 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 326, 362 |
| BstNI | CCWGG | 3 cut(s) | 78, 249, 414 |
| BstNSI | RCATGY | 1 cut(s) | 89 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 66 |
| BstSCI | CCNGG | 3 cut(s) | 76, 247, 412 |
| BstV1I | GCAGC | 2 cut(s) | 95, 203 |
| BstX2I | RGATCY | 1 cut(s) | 73 |
| BstYI | RGATCY | 1 cut(s) | 73 |
| BsuRI | GGCC | 1 cut(s) | 343 |
| BtgZI | GCGATG | 1 cut(s) | 303 |
| BtsIMutI | CAGTG | 1 cut(s) | 234 |
| Cac8I | GCNNGC | 1 cut(s) | 28 |
| Cfr13I | GGNCC | 3 cut(s) | 251, 311, 341 |
| Csp6I | GTAC | 2 cut(s) | 32, 42 |
| CviAII | CATG | 2 cut(s) | 86, 424 |
| CviJI | RGCY | 5 cut(s) | 26, 216, 247, 329, 343 |
| CviKI_1 | RGCY | 5 cut(s) | 26, 216, 247, 329, 343 |
| CviQI | GTAC | 2 cut(s) | 32, 42 |
| DpnI | GATC | 1 cut(s) | 75 |
| DpnII | GATC | 1 cut(s) | 73 |
| DraI | TTTAAA | 1 cut(s) | 391 |
| EciI | GGCGGA | 1 cut(s) | 96 |
| Eco47I | GGWCC | 2 cut(s) | 251, 311 |
| Eco72I | CACGTG | 1 cut(s) | 66 |
| Eco88I | CYCGRG | 1 cut(s) | 399 |
| EcoRII | CCWGG | 3 cut(s) | 76, 247, 412 |
| FaeI | CATG | 2 cut(s) | 89, 427 |
| FaiI | YATR | 5 cut(s) | 11, 87, 138, 156, 425 |
| FaqI | GGGAC | 1 cut(s) | 409 |
| FatI | CATG | 2 cut(s) | 85, 423 |
| FauI | CCCGC | 1 cut(s) | 325 |
| Fnu4HI | GCNGC | 2 cut(s) | 109, 217 |
| Fsp4HI | GCNGC | 2 cut(s) | 109, 217 |
| FspBI | CTAG | 3 cut(s) | 27, 122, 285 |
| GluI | GCNGC | 2 cut(s) | 109, 217 |
| HaeIII | GGCC | 1 cut(s) | 343 |
| HapII | CCGG | 1 cut(s) | 71 |
| Hin1II | CATG | 2 cut(s) | 89, 427 |
| HincII | GTYRAC | 2 cut(s) | 53, 406 |
| HindII | GTYRAC | 2 cut(s) | 53, 406 |
| HinfI | GANTC | 1 cut(s) | 397 |
| HpaII | CCGG | 1 cut(s) | 71 |
| HphI | GGTGA | 2 cut(s) | 31, 314 |
| Hpy166II | GTNNAC | 4 cut(s) | 42, 53, 406, 457 |
| Hpy188III | TCNNGA | 2 cut(s) | 71, 211 |
| Hpy8I | GTNNAC | 4 cut(s) | 42, 53, 406, 457 |
| HpyCH4IV | ACGT | 1 cut(s) | 65 |
| HpyCH4V | TGCA | 2 cut(s) | 9, 111 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 326, 362 |
| HpySE526I | ACGT | 1 cut(s) | 65 |
| Hsp92II | CATG | 2 cut(s) | 89, 427 |
| Kpn2I | TCCGGA | 1 cut(s) | 70 |
| Kzo9I | GATC | 1 cut(s) | 73 |
| Lsp1109I | GCAGC | 2 cut(s) | 95, 203 |
| MaeI | CTAG | 3 cut(s) | 27, 122, 285 |
| MaeII | ACGT | 1 cut(s) | 65 |
| MaeIII | GTNAC | 1 cut(s) | 19 |
| MalI | GATC | 1 cut(s) | 75 |
| MboI | GATC | 1 cut(s) | 73 |
| MflI | RGATCY | 1 cut(s) | 73 |
| MlyI | GAGTC | 1 cut(s) | 391 |
| MnlI | CCTC | 8 cut(s) | 10, 232, 264, 286, 324, 354, 391, 420 |
| MroI | TCCGGA | 1 cut(s) | 70 |
| MroXI | GAANNNNTTC | 1 cut(s) | 446 |
| MseI | TTAA | 1 cut(s) | 390 |
| MspA1I | CMGCKG | 1 cut(s) | 320 |
| MspI | CCGG | 1 cut(s) | 71 |
| MspR9I | CCNGG | 3 cut(s) | 78, 249, 414 |
| MvaI | CCWGG | 3 cut(s) | 78, 249, 414 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 326, 362 |
| NdeII | GATC | 1 cut(s) | 73 |
| NheI | GCTAGC | 1 cut(s) | 26 |
| NlaIII | CATG | 2 cut(s) | 89, 427 |
| NlaIV | GGNNCC | 1 cut(s) | 75 |
| NmuCI | GTSAC | 1 cut(s) | 19 |
| NspI | RCATGY | 1 cut(s) | 89 |
| PciI | ACATGT | 1 cut(s) | 85 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 216 |
| PdmI | GAANNNNTTC | 1 cut(s) | 446 |
| PkrI | GCNGC | 2 cut(s) | 110, 218 |
| PleI | GAGTC | 1 cut(s) | 391 |
| PmaCI | CACGTG | 1 cut(s) | 66 |
| PmlI | CACGTG | 1 cut(s) | 66 |
| PpsI | GAGTC | 1 cut(s) | 391 |
| Ppu21I | YACGTR | 1 cut(s) | 66 |
| PscI | ACATGT | 1 cut(s) | 85 |
| PshAI | GACNNNNGTC | 1 cut(s) | 66 |
| Psp6I | CCWGG | 3 cut(s) | 76, 247, 412 |
| PspCI | CACGTG | 1 cut(s) | 66 |
| PspGI | CCWGG | 3 cut(s) | 76, 247, 412 |
| PspN4I | GGNNCC | 1 cut(s) | 75 |
| PspPI | GGNCC | 3 cut(s) | 251, 311, 341 |
| PsuI | RGATCY | 1 cut(s) | 73 |
| RsaI | GTAC | 2 cut(s) | 33, 43 |
| RsaNI | GTAC | 2 cut(s) | 32, 42 |
| SaqAI | TTAA | 1 cut(s) | 390 |
| SatI | GCNGC | 2 cut(s) | 109, 217 |
| Sau3AI | GATC | 1 cut(s) | 73 |
| Sau96I | GGNCC | 3 cut(s) | 251, 311, 341 |
| SchI | GAGTC | 1 cut(s) | 391 |
| ScrFI | CCNGG | 3 cut(s) | 78, 249, 414 |
| SetI | ASST | 7 cut(s) | 21, 68, 218, 243, 304, 316, 340 |
| SinI | GGWCC | 2 cut(s) | 251, 311 |
| SsiI | CCGC | 2 cut(s) | 81, 318 |
| SspI | AATATT | 1 cut(s) | 163 |
| SspMI | CTAG | 3 cut(s) | 27, 122, 285 |
| StyD4I | CCNGG | 3 cut(s) | 76, 247, 412 |
| TaiI | ACGT | 1 cut(s) | 68 |
| TaqI | TCGA | 1 cut(s) | 210 |
| TatI | WGTACW | 1 cut(s) | 41 |
| Tru1I | TTAA | 1 cut(s) | 390 |
| Tru9I | TTAA | 1 cut(s) | 390 |
| TscAI | CASTG | 1 cut(s) | 234 |
| TseFI | GTSAC | 1 cut(s) | 19 |
| TseI | GCWGC | 2 cut(s) | 108, 216 |
| Tsp45I | GTSAC | 1 cut(s) | 19 |
| TspDTI | ATGAA | 1 cut(s) | 84 |
| TspGWI | ACGGA | 1 cut(s) | 195 |
| TspRI | CASTG | 1 cut(s) | 234 |
| VpaK11BI | GGWCC | 2 cut(s) | 251, 311 |
| XceI | RCATGY | 1 cut(s) | 89 |
| XmnI | GAANNNNTTC | 1 cut(s) | 446 |
| XspI | CTAG | 3 cut(s) | 27, 122, 285 |