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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 153 |
| AccII | CGCG | 2 cut(s) | 117, 275 |
| AciI | CCGC | 4 cut(s) | 117, 207, 273, 305 |
| AfaI | GTAC | 1 cut(s) | 231 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 163, 219, 220, 305, 306, 359 |
| AjnI | CCWGG | 2 cut(s) | 182, 236 |
| AluBI | AGCT | 1 cut(s) | 136 |
| AluI | AGCT | 1 cut(s) | 136 |
| Alw21I | GWGCWC | 1 cut(s) | 138 |
| AoxI | GGCC | 1 cut(s) | 129 |
| AspLEI | GCGC | 2 cut(s) | 117, 312 |
| AspS9I | GGNCC | 5 cut(s) | 36, 91, 129, 186, 210 |
| AsuC2I | CCSGG | 1 cut(s) | 34 |
| AsuHPI | GGTGA | 3 cut(s) | 250, 262, 352 |
| AvaII | GGWCC | 4 cut(s) | 36, 91, 186, 210 |
| BanII | GRGCYC | 1 cut(s) | 138 |
| BauI | CACGAG | 1 cut(s) | 137 |
| BbsI | GAAGAC | 1 cut(s) | 35 |
| Bbv12I | GWGCWC | 1 cut(s) | 138 |
| BccI | CCATC | 2 cut(s) | 50, 337 |
| BciT130I | CCWGG | 2 cut(s) | 184, 238 |
| BciVI | GTATCC | 1 cut(s) | 342 |
| BcnI | CCSGG | 1 cut(s) | 34 |
| BfaI | CTAG | 2 cut(s) | 17, 158 |
| BfoI | RGCGCY | 1 cut(s) | 313 |
| BfuI | GTATCC | 1 cut(s) | 342 |
| Bme1390I | CCNGG | 3 cut(s) | 34, 184, 238 |
| Bme18I | GGWCC | 4 cut(s) | 36, 91, 186, 210 |
| BmgT120I | GGNCC | 5 cut(s) | 36, 91, 129, 186, 210 |
| BmiI | GGNNCC | 5 cut(s) | 93, 122, 188, 211, 212 |
| BmrFI | CCNGG | 3 cut(s) | 34, 184, 238 |
| BmsI | GCATC | 3 cut(s) | 288, 292, 324 |
| BpiI | GAAGAC | 1 cut(s) | 35 |
| BpuMI | CCSGG | 1 cut(s) | 34 |
| Bsa29I | ATCGAT | 1 cut(s) | 332 |
| BsaJI | CCNNGG | 1 cut(s) | 214 |
| BsaWI | WCCGGW | 1 cut(s) | 88 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 163, 219, 220, 305, 306, 359 |
| Bse118I | RCCGGY | 1 cut(s) | 337 |
| BseBI | CCWGG | 2 cut(s) | 184, 238 |
| BseCI | ATCGAT | 1 cut(s) | 332 |
| BseDI | CCNNGG | 1 cut(s) | 214 |
| BseGI | GGATG | 1 cut(s) | 75 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 163, 219, 220, 305, 306, 359 |
| BseRI | GAGGAG | 1 cut(s) | 358 |
| Bsh1236I | CGCG | 2 cut(s) | 117, 275 |
| BshFI | GGCC | 1 cut(s) | 131 |
| BshVI | ATCGAT | 1 cut(s) | 332 |
| BsiHKAI | GWGCWC | 1 cut(s) | 138 |
| BsiSI | CCGG | 4 cut(s) | 34, 89, 204, 338 |
| BslFI | GGGAC | 2 cut(s) | 172, 196 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 163, 219, 220, 305, 306, 359 |
| BsmFI | GGGAC | 2 cut(s) | 172, 196 |
| BsnI | GGCC | 1 cut(s) | 131 |
| Bsp1286I | GDGCHC | 1 cut(s) | 138 |
| Bsp143I | GATC | 1 cut(s) | 3 |
| BspACI | CCGC | 4 cut(s) | 117, 207, 273, 305 |
| BspANI | GGCC | 1 cut(s) | 131 |
| BspDI | ATCGAT | 1 cut(s) | 332 |
| BspFNI | CGCG | 2 cut(s) | 117, 275 |
| BspLI | GGNNCC | 5 cut(s) | 93, 122, 188, 211, 212 |
| BsrFI | RCCGGY | 1 cut(s) | 337 |
| BssAI | RCCGGY | 1 cut(s) | 337 |
| BssECI | CCNNGG | 1 cut(s) | 214 |
| BssMI | GATC | 1 cut(s) | 3 |
| BssSI | CACGAG | 1 cut(s) | 137 |
| BssT1I | CCWWGG | 1 cut(s) | 214 |
| Bst2BI | CACGAG | 1 cut(s) | 137 |
| Bst2UI | CCWGG | 2 cut(s) | 184, 238 |
| Bst4CI | ACNGT | 1 cut(s) | 262 |
| Bst6I | CTCTTC | 1 cut(s) | 238 |
| BstC8I | GCNNGC | 1 cut(s) | 277 |
| BstEII | GGTNACC | 1 cut(s) | 142 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 161 |
| BstF5I | GGATG | 1 cut(s) | 75 |
| BstFNI | CGCG | 2 cut(s) | 117, 275 |
| BstH2I | RGCGCY | 1 cut(s) | 313 |
| BstHHI | GCGC | 2 cut(s) | 117, 312 |
| BstKTI | GATC | 1 cut(s) | 6 |
| BstMBI | GATC | 1 cut(s) | 3 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 334 |
| BstNI | CCWGG | 2 cut(s) | 184, 238 |
| BstPI | GGTNACC | 1 cut(s) | 142 |
| BstSCI | CCNGG | 3 cut(s) | 32, 182, 236 |
| BstUI | CGCG | 2 cut(s) | 117, 275 |
| BstV2I | GAAGAC | 1 cut(s) | 35 |
| Bsu15I | ATCGAT | 1 cut(s) | 332 |
| BsuI | GTATCC | 1 cut(s) | 342 |
| BsuRI | GGCC | 1 cut(s) | 131 |
| BsuTUI | ATCGAT | 1 cut(s) | 332 |
| BtsCI | GGATG | 1 cut(s) | 75 |
| Cac8I | GCNNGC | 1 cut(s) | 277 |
| CfoI | GCGC | 2 cut(s) | 117, 312 |
| Cfr10I | RCCGGY | 1 cut(s) | 337 |
| Cfr13I | GGNCC | 5 cut(s) | 36, 91, 129, 186, 210 |
| ClaI | ATCGAT | 1 cut(s) | 332 |
| CsiI | ACCWGGT | 1 cut(s) | 182 |
| Csp6I | GTAC | 1 cut(s) | 230 |
| CviJI | RGCY | 3 cut(s) | 131, 136, 376 |
| CviKI_1 | RGCY | 3 cut(s) | 131, 136, 376 |
| CviQI | GTAC | 1 cut(s) | 230 |
| DpnI | GATC | 1 cut(s) | 5 |
| DpnII | GATC | 1 cut(s) | 3 |
| Eam1104I | CTCTTC | 1 cut(s) | 238 |
| EarI | CTCTTC | 1 cut(s) | 238 |
| Ecl136II | GAGCTC | 1 cut(s) | 136 |
| Eco130I | CCWWGG | 1 cut(s) | 214 |
| Eco24I | GRGCYC | 1 cut(s) | 138 |
| Eco32I | GATATC | 1 cut(s) | 52 |
| Eco47I | GGWCC | 4 cut(s) | 36, 91, 186, 210 |
| Eco53kI | GAGCTC | 1 cut(s) | 136 |
| Eco91I | GGTNACC | 1 cut(s) | 142 |
| EcoICRI | GAGCTC | 1 cut(s) | 136 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 161 |
| EcoO109I | RGGNCCY | 2 cut(s) | 186, 210 |
| EcoO65I | GGTNACC | 1 cut(s) | 142 |
| EcoRII | CCWGG | 2 cut(s) | 182, 236 |
| EcoRV | GATATC | 1 cut(s) | 52 |
| EcoT14I | CCWWGG | 1 cut(s) | 214 |
| EcoT38I | GRGCYC | 1 cut(s) | 138 |
| ErhI | CCWWGG | 1 cut(s) | 214 |
| FaiI | YATR | 4 cut(s) | 11, 81, 315, 326 |
| FaqI | GGGAC | 2 cut(s) | 172, 196 |
| FauI | CCCGC | 3 cut(s) | 110, 200, 298 |
| FblI | GTMKAC | 1 cut(s) | 153 |
| FokI | GGATG | 1 cut(s) | 82 |
| FriOI | GRGCYC | 1 cut(s) | 138 |
| FspBI | CTAG | 2 cut(s) | 17, 158 |
| GlaI | GCGC | 2 cut(s) | 116, 311 |
| HaeII | RGCGCY | 1 cut(s) | 313 |
| HaeIII | GGCC | 1 cut(s) | 131 |
| HapII | CCGG | 4 cut(s) | 34, 89, 204, 338 |
| HhaI | GCGC | 2 cut(s) | 117, 312 |
| Hin6I | GCGC | 2 cut(s) | 115, 310 |
| HinP1I | GCGC | 2 cut(s) | 115, 310 |
| HinfI | GANTC | 1 cut(s) | 255 |
| HpaII | CCGG | 4 cut(s) | 34, 89, 204, 338 |
| HphI | GGTGA | 3 cut(s) | 250, 262, 352 |
| Hpy166II | GTNNAC | 2 cut(s) | 154, 371 |
| Hpy8I | GTNNAC | 2 cut(s) | 154, 371 |
| HpyAV | CCTTC | 3 cut(s) | 134, 157, 353 |
| HpyCH4III | ACNGT | 1 cut(s) | 262 |
| HpyCH4IV | ACGT | 1 cut(s) | 320 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 334 |
| HpySE526I | ACGT | 1 cut(s) | 320 |
| HspAI | GCGC | 2 cut(s) | 115, 310 |
| KflI | GGGWCCC | 1 cut(s) | 210 |
| Kzo9I | GATC | 1 cut(s) | 3 |
| LpnPI | CCDG | 9 cut(s) | 47, 68, 102, 169, 196, 217, 223, 250, 351 |
| LweI | GCATC | 3 cut(s) | 288, 292, 324 |
| MabI | ACCWGGT | 1 cut(s) | 182 |
| MaeI | CTAG | 2 cut(s) | 17, 158 |
| MaeII | ACGT | 1 cut(s) | 320 |
| MaeIII | GTNAC | 2 cut(s) | 142, 268 |
| MalI | GATC | 1 cut(s) | 5 |
| MboI | GATC | 1 cut(s) | 3 |
| MboII | GAAGA | 2 cut(s) | 40, 255 |
| MhlI | GDGCHC | 1 cut(s) | 138 |
| MluCI | AATT | 1 cut(s) | 249 |
| MmeI | TCCRAC | 1 cut(s) | 39 |
| MnlI | CCTC | 9 cut(s) | 13, 40, 49, 66, 217, 336, 339, 356, 387 |
| MspI | CCGG | 4 cut(s) | 34, 89, 204, 338 |
| MspR9I | CCNGG | 3 cut(s) | 34, 184, 238 |
| MvaI | CCWGG | 2 cut(s) | 184, 238 |
| MvnI | CGCG | 2 cut(s) | 117, 275 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 334 |
| NciI | CCSGG | 1 cut(s) | 34 |
| NdeII | GATC | 1 cut(s) | 3 |
| NlaIV | GGNNCC | 5 cut(s) | 93, 122, 188, 211, 212 |
| NmeAIII | GCCGAG | 2 cut(s) | 157, 360 |
| NmuCI | GTSAC | 1 cut(s) | 268 |
| PfeI | GAWTC | 1 cut(s) | 255 |
| PpuMI | RGGWCCY | 2 cut(s) | 186, 210 |
| Psp124BI | GAGCTC | 1 cut(s) | 138 |
| Psp5II | RGGWCCY | 2 cut(s) | 186, 210 |
| Psp6I | CCWGG | 2 cut(s) | 182, 236 |
| PspEI | GGTNACC | 1 cut(s) | 142 |
| PspGI | CCWGG | 2 cut(s) | 182, 236 |
| PspN4I | GGNNCC | 5 cut(s) | 93, 122, 188, 211, 212 |
| PspPI | GGNCC | 5 cut(s) | 36, 91, 129, 186, 210 |
| PspPPI | RGGWCCY | 2 cut(s) | 186, 210 |
| RsaI | GTAC | 1 cut(s) | 231 |
| RsaNI | GTAC | 1 cut(s) | 230 |
| SacI | GAGCTC | 1 cut(s) | 138 |
| Sau3AI | GATC | 1 cut(s) | 3 |
| Sau96I | GGNCC | 5 cut(s) | 36, 91, 129, 186, 210 |
| ScrFI | CCNGG | 3 cut(s) | 34, 184, 238 |
| SduI | GDGCHC | 1 cut(s) | 138 |
| SetI | ASST | 7 cut(s) | 18, 41, 126, 138, 159, 188, 323 |
| SexAI | ACCWGGT | 1 cut(s) | 182 |
| SfaNI | GCATC | 3 cut(s) | 288, 292, 324 |
| SinI | GGWCC | 4 cut(s) | 36, 91, 186, 210 |
| Sse9I | AATT | 1 cut(s) | 249 |
| SsiI | CCGC | 4 cut(s) | 117, 207, 273, 305 |
| SspMI | CTAG | 2 cut(s) | 17, 158 |
| SstI | GAGCTC | 1 cut(s) | 138 |
| StyD4I | CCNGG | 3 cut(s) | 32, 182, 236 |
| StyI | CCWWGG | 1 cut(s) | 214 |
| TaaI | ACNGT | 1 cut(s) | 262 |
| TaiI | ACGT | 1 cut(s) | 323 |
| TaqI | TCGA | 3 cut(s) | 6, 45, 332 |
| TasI | AATT | 1 cut(s) | 249 |
| TfiI | GAWTC | 1 cut(s) | 255 |
| TseFI | GTSAC | 1 cut(s) | 268 |
| Tsp45I | GTSAC | 1 cut(s) | 268 |
| TspGWI | ACGGA | 1 cut(s) | 82 |
| VpaK11BI | GGWCC | 4 cut(s) | 36, 91, 186, 210 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 161 |
| XmiI | GTMKAC | 1 cut(s) | 153 |
| XspI | CTAG | 2 cut(s) | 17, 158 |