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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 2558 |
| Acc65I | GGTACC | 3 cut(s) | 58, 383, 1403 |
| AccB1I | GGYRCC | 5 cut(s) | 58, 383, 1086, 1403, 2672 |
| AccBSI | CCGCTC | 2 cut(s) | 2305, 2650 |
| AccI | GTMKAC | 3 cut(s) | 492, 732, 1674 |
| AccII | CGCG | 5 cut(s) | 170, 974, 1221, 1223, 2480 |
| AclWI | GGATC | 9 cut(s) | 436, 586, 676, 826, 1748, 1902, 2014, 2142, 2475 |
| AcoI | YGGCCR | 7 cut(s) | 606, 846, 1008, 1794, 1954, 2328, 2589 |
| AcsI | RAATTY | 4 cut(s) | 44, 131, 1141, 2612 |
| AcuI | CTGAAG | 5 cut(s) | 480, 720, 974, 1464, 2294 |
| AcyI | GRCGYC | 2 cut(s) | 1924, 2250 |
| AdeI | CACNNNGTG | 1 cut(s) | 1780 |
| AgeI | ACCGGT | 3 cut(s) | 474, 714, 1400 |
| AgsI | TTSAA | 4 cut(s) | 131, 1412, 1709, 2317 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1681 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 500, 532, 740, 772 |
| Alw21I | GWGCWC | 1 cut(s) | 1505 |
| Alw26I | GTCTC | 7 cut(s) | 183, 382, 1706, 2204, 2440, 2630, 2688 |
| AlwI | GGATC | 9 cut(s) | 436, 586, 676, 826, 1748, 1902, 2014, 2142, 2475 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 1099, 2083, 2429 |
| Ama87I | CYCGRG | 7 cut(s) | 614, 854, 1024, 1467, 2002, 2205, 2400 |
| ApoI | RAATTY | 4 cut(s) | 44, 131, 1141, 2612 |
| AsiGI | ACCGGT | 3 cut(s) | 474, 714, 1400 |
| Asp718I | GGTACC | 3 cut(s) | 58, 383, 1403 |
| AspLEI | GCGC | 3 cut(s) | 1039, 1223, 1885 |
| AspS9I | GGNCC | 9 cut(s) | 516, 756, 894, 1180, 1701, 1771, 1918, 2012, 2121 |
| AvaI | CYCGRG | 7 cut(s) | 614, 854, 1024, 1467, 2002, 2205, 2400 |
| AvaII | GGWCC | 7 cut(s) | 516, 756, 894, 1180, 1701, 1918, 2121 |
| BaeGI | GKGCMC | 1 cut(s) | 2227 |
| BalI | TGGCCA | 2 cut(s) | 608, 848 |
| BanI | GGYRCC | 5 cut(s) | 58, 383, 1086, 1403, 2672 |
| BanII | GRGCYC | 1 cut(s) | 1505 |
| BauI | CACGAG | 3 cut(s) | 1043, 1693, 2489 |
| Bbv12I | GWGCWC | 1 cut(s) | 1505 |
| BbvCI | CCTCAGC | 1 cut(s) | 2373 |
| BceAI | ACGGC | 8 cut(s) | 335, 995, 1463, 1999, 2030, 2181, 2417, 2480 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 2654, 2688 |
| BciVI | GTATCC | 5 cut(s) | 415, 655, 957, 1733, 2257 |
| BcoDI | GTCTC | 7 cut(s) | 183, 382, 1706, 2204, 2440, 2630, 2688 |
| BfaI | CTAG | 5 cut(s) | 1284, 1301, 1355, 1541, 2600 |
| BfmI | CTRYAG | 2 cut(s) | 1091, 2421 |
| BfoI | RGCGCY | 2 cut(s) | 1040, 1886 |
| BfuI | GTATCC | 5 cut(s) | 415, 655, 957, 1733, 2257 |
| BglII | AGATCT | 1 cut(s) | 286 |
| BlpI | GCTNAGC | 1 cut(s) | 2352 |
| Bme18I | GGWCC | 7 cut(s) | 516, 756, 894, 1180, 1701, 1918, 2121 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1681 |
| BmeT110I | CYCGRG | 7 cut(s) | 614, 854, 1024, 1467, 2002, 2205, 2400 |
| BmgT120I | GGNCC | 9 cut(s) | 516, 756, 894, 1180, 1701, 1771, 1918, 2012, 2121 |
| BmrI | ACTGGG | 3 cut(s) | 534, 774, 1878 |
| BmsI | GCATC | 4 cut(s) | 106, 1098, 1645, 2208 |
| BmuI | ACTGGG | 3 cut(s) | 534, 774, 1878 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1923 |
| BplI | GAGNNNNNCTC | 8 cut(s) | 69, 101, 1487, 1519, 2294, 2326, 2513, 2545 |
| BpmI | CTGGAG | 4 cut(s) | 2159, 2315, 2483, 2651 |
| Bpu10I | CCTNAGC | 4 cut(s) | 184, 627, 867, 2373 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 2352 |
| BpuEI | CTTGAG | 2 cut(s) | 1228, 2378 |
| Bsa29I | ATCGAT | 1 cut(s) | 2213 |
| BsaBI | GATNNNNATC | 1 cut(s) | 2700 |
| BsaHI | GRCGYC | 2 cut(s) | 1924, 2250 |
| BsaI | GGTCTC | 4 cut(s) | 382, 1706, 2630, 2688 |
| BsaWI | WCCGGW | 7 cut(s) | 55, 380, 474, 592, 714, 967, 1400 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1931, 1961, 2495, 2525 |
| Bse118I | RCCGGY | 7 cut(s) | 474, 714, 1400, 1820, 1837, 1920, 2675 |
| Bse1I | ACTGG | 7 cut(s) | 439, 529, 679, 769, 1873, 1957, 2253 |
| Bse8I | GATNNNNATC | 1 cut(s) | 2700 |
| BseCI | ATCGAT | 1 cut(s) | 2213 |
| BseGI | GGATG | 7 cut(s) | 402, 642, 1003, 1113, 1515, 1618, 1966 |
| BseJI | GATNNNNATC | 1 cut(s) | 2700 |
| BseMII | CTCAG | 6 cut(s) | 198, 2313, 2343, 2364, 2529, 2699 |
| BseNI | ACTGG | 7 cut(s) | 439, 529, 679, 769, 1873, 1957, 2253 |
| BseRI | GAGGAG | 6 cut(s) | 1513, 1613, 2273, 2294, 2399, 2516 |
| BseSI | GKGCMC | 1 cut(s) | 2227 |
| BseX3I | CGGCCG | 1 cut(s) | 2589 |
| BseYI | CCCAGC | 1 cut(s) | 1029 |
| BsgI | GTGCAG | 3 cut(s) | 1025, 1646, 1802 |
| Bsh1236I | CGCG | 5 cut(s) | 170, 974, 1221, 1223, 2480 |
| Bsh1285I | CGRYCG | 3 cut(s) | 475, 715, 2592 |
| BshNI | GGYRCC | 5 cut(s) | 58, 383, 1086, 1403, 2672 |
| BshTI | ACCGGT | 3 cut(s) | 474, 714, 1400 |
| BshVI | ATCGAT | 1 cut(s) | 2213 |
| BsiEI | CGRYCG | 3 cut(s) | 475, 715, 2592 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1505 |
| BsiHKCI | CYCGRG | 7 cut(s) | 614, 854, 1024, 1467, 2002, 2205, 2400 |
| BslFI | GGGAC | 5 cut(s) | 227, 907, 1193, 2321, 2417 |
| BsmAI | GTCTC | 7 cut(s) | 183, 382, 1706, 2204, 2440, 2630, 2688 |
| BsmBI | CGTCTC | 2 cut(s) | 2204, 2440 |
| BsmFI | GGGAC | 5 cut(s) | 227, 907, 1193, 2321, 2417 |
| BsmI | GAATGC | 3 cut(s) | 621, 861, 2401 |
| Bso31I | GGTCTC | 4 cut(s) | 382, 1706, 2630, 2688 |
| BsoBI | CYCGRG | 7 cut(s) | 614, 854, 1024, 1467, 2002, 2205, 2400 |
| Bsp1286I | GDGCHC | 2 cut(s) | 1505, 2227 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 2352 |
| Bsp19I | CCATGG | 1 cut(s) | 1791 |
| Bsp68I | TCGCGA | 1 cut(s) | 170 |
| BspCNI | CTCAG | 6 cut(s) | 197, 2314, 2344, 2365, 2528, 2698 |
| BspDI | ATCGAT | 1 cut(s) | 2213 |
| BspFNI | CGCG | 5 cut(s) | 170, 974, 1221, 1223, 2480 |
| BspHI | TCATGA | 1 cut(s) | 2565 |
| BspMAI | CTGCAG | 1 cut(s) | 2425 |
| BspPI | GGATC | 9 cut(s) | 436, 586, 676, 826, 1748, 1902, 2014, 2142, 2475 |
| BspT107I | GGYRCC | 5 cut(s) | 58, 383, 1086, 1403, 2672 |
| BspTNI | GGTCTC | 4 cut(s) | 382, 1706, 2630, 2688 |
| BsrBI | CCGCTC | 2 cut(s) | 2305, 2650 |
| BsrFI | RCCGGY | 7 cut(s) | 474, 714, 1400, 1820, 1837, 1920, 2675 |
| BsrI | ACTGG | 7 cut(s) | 439, 529, 679, 769, 1873, 1957, 2253 |
| BssAI | RCCGGY | 7 cut(s) | 474, 714, 1400, 1820, 1837, 1920, 2675 |
| BssNI | GRCGYC | 2 cut(s) | 1924, 2250 |
| BssSI | CACGAG | 3 cut(s) | 1043, 1693, 2489 |
| BssT1I | CCWWGG | 6 cut(s) | 209, 226, 345, 1060, 1791, 2026 |
| Bst2BI | CACGAG | 3 cut(s) | 1043, 1693, 2489 |
| Bst4CI | ACNGT | 9 cut(s) | 110, 152, 265, 388, 844, 898, 1095, 1270, 1745 |
| Bst6I | CTCTTC | 5 cut(s) | 1451, 1568, 1704, 1861, 1898 |
| BstACI | GRCGYC | 2 cut(s) | 1924, 2250 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 2429 |
| BstC8I | GCNNGC | 8 cut(s) | 456, 696, 885, 1839, 2223, 2538, 2662, 2677 |
| BstDSI | CCRYGG | 3 cut(s) | 1791, 2478, 2496 |
| BstEII | GGTNACC | 1 cut(s) | 1270 |
| BstENI | CCTNNNNNAGG | 4 cut(s) | 448, 688, 2156, 2276 |
| BstF5I | GGATG | 7 cut(s) | 402, 642, 1003, 1113, 1515, 1618, 1966 |
| BstFNI | CGCG | 5 cut(s) | 170, 974, 1221, 1223, 2480 |
| BstH2I | RGCGCY | 2 cut(s) | 1040, 1886 |
| BstHHI | GCGC | 3 cut(s) | 1039, 1223, 1885 |
| BstMAI | GTCTC | 7 cut(s) | 183, 382, 1706, 2204, 2440, 2630, 2688 |
| BstMCI | CGRYCG | 3 cut(s) | 475, 715, 2592 |
| BstNSI | RCATGY | 2 cut(s) | 949, 1636 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1923 |
| BstPI | GGTNACC | 1 cut(s) | 1270 |
| BstSFI | CTRYAG | 2 cut(s) | 1091, 2421 |
| BstSLI | GKGCMC | 1 cut(s) | 2227 |
| BstUI | CGCG | 5 cut(s) | 170, 974, 1221, 1223, 2480 |
| BstX2I | RGATCY | 5 cut(s) | 286, 578, 818, 1907, 2134 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 70, 1973 |
| BstYI | RGATCY | 5 cut(s) | 286, 578, 818, 1907, 2134 |
| BstZI | CGGCCG | 1 cut(s) | 2589 |
| Bsu15I | ATCGAT | 1 cut(s) | 2213 |
| BsuI | GTATCC | 5 cut(s) | 415, 655, 957, 1733, 2257 |
| BsuTUI | ATCGAT | 1 cut(s) | 2213 |
| BtgI | CCRYGG | 3 cut(s) | 1791, 2478, 2496 |
| BtgZI | GCGATG | 1 cut(s) | 1890 |
| BtsCI | GGATG | 7 cut(s) | 402, 642, 1003, 1113, 1515, 1618, 1966 |
| BtsIMutI | CAGTG | 3 cut(s) | 849, 1950, 2260 |
| BtuMI | TCGCGA | 1 cut(s) | 170 |
| Cac8I | GCNNGC | 8 cut(s) | 456, 696, 885, 1839, 2223, 2538, 2662, 2677 |
| CaiI | CAGNNNCTG | 3 cut(s) | 1099, 2083, 2429 |
| CciI | TCATGA | 1 cut(s) | 2565 |
| CfoI | GCGC | 3 cut(s) | 1039, 1223, 1885 |
| Cfr10I | RCCGGY | 7 cut(s) | 474, 714, 1400, 1820, 1837, 1920, 2675 |
| Cfr13I | GGNCC | 9 cut(s) | 516, 756, 894, 1180, 1701, 1771, 1918, 2012, 2121 |
| Cfr42I | CCGCGG | 1 cut(s) | 2481 |
| Cfr9I | CCCGGG | 5 cut(s) | 1024, 1467, 2002, 2205, 2400 |
| ClaI | ATCGAT | 1 cut(s) | 2213 |
| CseI | GACGC | 5 cut(s) | 980, 1625, 1769, 1913, 2258 |
| CsiI | ACCWGGT | 1 cut(s) | 2157 |
| CspAI | ACCGGT | 3 cut(s) | 474, 714, 1400 |
| CviAII | CATG | 9 cut(s) | 922, 946, 1633, 1792, 1825, 2125, 2439, 2533, 2566 |
| DraIII | CACNNNGTG | 1 cut(s) | 1780 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 2558 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1681 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 2558 |
| EaeI | YGGCCR | 7 cut(s) | 606, 846, 1008, 1794, 1954, 2328, 2589 |
| EagI | CGGCCG | 1 cut(s) | 2589 |
| Eam1104I | CTCTTC | 5 cut(s) | 1451, 1568, 1704, 1861, 1898 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1681 |
| EarI | CTCTTC | 5 cut(s) | 1451, 1568, 1704, 1861, 1898 |
| Ecl136II | GAGCTC | 1 cut(s) | 1503 |
| EclXI | CGGCCG | 1 cut(s) | 2589 |
| Eco130I | CCWWGG | 6 cut(s) | 209, 226, 345, 1060, 1791, 2026 |
| Eco24I | GRGCYC | 1 cut(s) | 1505 |
| Eco31I | GGTCTC | 4 cut(s) | 382, 1706, 2630, 2688 |
| Eco32I | GATATC | 1 cut(s) | 2146 |
| Eco47I | GGWCC | 7 cut(s) | 516, 756, 894, 1180, 1701, 1918, 2121 |
| Eco52I | CGGCCG | 1 cut(s) | 2589 |
| Eco53kI | GAGCTC | 1 cut(s) | 1503 |
| Eco57I | CTGAAG | 5 cut(s) | 480, 720, 974, 1464, 2294 |
| Eco88I | CYCGRG | 7 cut(s) | 614, 854, 1024, 1467, 2002, 2205, 2400 |
| Eco91I | GGTNACC | 1 cut(s) | 1270 |
| EcoICRI | GAGCTC | 1 cut(s) | 1503 |
| EcoNI | CCTNNNNNAGG | 4 cut(s) | 448, 688, 2156, 2276 |
| EcoO109I | RGGNCCY | 3 cut(s) | 516, 756, 1180 |
| EcoO65I | GGTNACC | 1 cut(s) | 1270 |
| EcoRV | GATATC | 1 cut(s) | 2146 |
| EcoT14I | CCWWGG | 6 cut(s) | 209, 226, 345, 1060, 1791, 2026 |
| EcoT38I | GRGCYC | 1 cut(s) | 1505 |
| ErhI | CCWWGG | 6 cut(s) | 209, 226, 345, 1060, 1791, 2026 |
| Esp3I | CGTCTC | 2 cut(s) | 2204, 2440 |
| FaeI | CATG | 9 cut(s) | 925, 949, 1636, 1795, 1828, 2128, 2442, 2536, 2569 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1701, 1733 |
| FaqI | GGGAC | 5 cut(s) | 227, 907, 1193, 2321, 2417 |
| FatI | CATG | 9 cut(s) | 921, 945, 1632, 1791, 1824, 2124, 2438, 2532, 2565 |
| FauI | CCCGC | 9 cut(s) | 1255, 2050, 2214, 2298, 2485, 2613, 2643, 2653, 2672 |
| FblI | GTMKAC | 3 cut(s) | 492, 732, 1674 |
| FokI | GGATG | 7 cut(s) | 409, 649, 1010, 1120, 1502, 1625, 1973 |
| FriOI | GRGCYC | 1 cut(s) | 1505 |
| FspBI | CTAG | 5 cut(s) | 1284, 1301, 1355, 1541, 2600 |
| GlaI | GCGC | 3 cut(s) | 1038, 1222, 1884 |
| GsaI | CCCAGC | 1 cut(s) | 1033 |
| GsuI | CTGGAG | 4 cut(s) | 2159, 2315, 2483, 2651 |
| HaeII | RGCGCY | 2 cut(s) | 1040, 1886 |
| HgaI | GACGC | 5 cut(s) | 980, 1625, 1769, 1913, 2258 |
| HhaI | GCGC | 3 cut(s) | 1039, 1223, 1885 |
| Hin1I | GRCGYC | 2 cut(s) | 1924, 2250 |
| Hin1II | CATG | 9 cut(s) | 925, 949, 1636, 1795, 1828, 2128, 2442, 2536, 2569 |
| Hin6I | GCGC | 3 cut(s) | 1037, 1221, 1883 |
| HinP1I | GCGC | 3 cut(s) | 1037, 1221, 1883 |
| HincII | GTYRAC | 1 cut(s) | 1675 |
| HindII | GTYRAC | 1 cut(s) | 1675 |
| HpyCH4III | ACNGT | 9 cut(s) | 110, 152, 265, 388, 844, 898, 1095, 1270, 1745 |
| HpyCH4IV | ACGT | 2 cut(s) | 1800, 1946 |
| HpyCH4V | TGCA | 5 cut(s) | 205, 1042, 1663, 1783, 2423 |
| HpySE526I | ACGT | 2 cut(s) | 1800, 1946 |
| Hsp92I | GRCGYC | 2 cut(s) | 1924, 2250 |
| Hsp92II | CATG | 9 cut(s) | 925, 949, 1636, 1795, 1828, 2128, 2442, 2536, 2569 |
| HspAI | GCGC | 3 cut(s) | 1037, 1221, 1883 |
| KpnI | GGTACC | 3 cut(s) | 62, 387, 1407 |
| KroI | GCCGGC | 2 cut(s) | 1837, 2675 |
| KroNI | GCCGGC | 2 cut(s) | 1839, 2677 |
| KspI | CCGCGG | 1 cut(s) | 2481 |
| LmnI | GCTCC | 8 cut(s) | 90, 595, 1059, 1500, 1560, 1768, 1872, 2302 |
| LweI | GCATC | 4 cut(s) | 106, 1098, 1645, 2208 |
| MabI | ACCWGGT | 1 cut(s) | 2157 |
| MaeI | CTAG | 5 cut(s) | 1284, 1301, 1355, 1541, 2600 |
| MaeII | ACGT | 2 cut(s) | 1800, 1946 |
| MaeIII | GTNAC | 6 cut(s) | 598, 838, 1270, 1303, 1942, 2016 |
| MbiI | CCGCTC | 2 cut(s) | 2305, 2650 |
| MflI | RGATCY | 5 cut(s) | 286, 578, 818, 1907, 2134 |
| MhlI | GDGCHC | 2 cut(s) | 1505, 2227 |
| MlsI | TGGCCA | 2 cut(s) | 608, 848 |
| MluCI | AATT | 9 cut(s) | 44, 131, 234, 390, 497, 737, 1141, 1803, 2612 |
| MluNI | TGGCCA | 2 cut(s) | 608, 848 |
| MlyI | GAGTC | 8 cut(s) | 560, 800, 1705, 2303, 2399, 2435, 2450, 2675 |
| MmeI | TCCRAC | 4 cut(s) | 102, 579, 819, 1467 |
| Mox20I | TGGCCA | 2 cut(s) | 608, 848 |
| MroNI | GCCGGC | 2 cut(s) | 1837, 2675 |
| MscI | TGGCCA | 2 cut(s) | 608, 848 |
| MseI | TTAA | 3 cut(s) | 135, 2232, 2574 |
| MslI | CAYNNNNRTG | 3 cut(s) | 926, 1637, 1823 |
| Msp20I | TGGCCA | 2 cut(s) | 608, 848 |
| MspA1I | CMGCKG | 5 cut(s) | 1132, 1396, 2080, 2344, 2480 |
| Mva1269I | GAATGC | 3 cut(s) | 621, 861, 2401 |
| MvnI | CGCG | 5 cut(s) | 170, 974, 1221, 1223, 2480 |
| NaeI | GCCGGC | 2 cut(s) | 1839, 2677 |
| NcoI | CCATGG | 1 cut(s) | 1791 |
| NgoMIV | GCCGGC | 2 cut(s) | 1837, 2675 |
| NlaIII | CATG | 9 cut(s) | 925, 949, 1636, 1795, 1828, 2128, 2442, 2536, 2569 |
| NmeAIII | GCCGAG | 3 cut(s) | 296, 657, 897 |
| NmuCI | GTSAC | 5 cut(s) | 598, 838, 1270, 1942, 2016 |
| NruI | TCGCGA | 1 cut(s) | 170 |
| NspI | RCATGY | 2 cut(s) | 949, 1636 |
| PagI | TCATGA | 1 cut(s) | 2565 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 518, 758, 1691 |
| PctI | GAATGC | 3 cut(s) | 621, 861, 2401 |
| PdiI | GCCGGC | 2 cut(s) | 1839, 2677 |
| PfeI | GAWTC | 7 cut(s) | 401, 641, 1015, 1048, 1512, 2191, 2210 |
| PfoI | TCCNGGA | 1 cut(s) | 2628 |
| PinAI | ACCGGT | 3 cut(s) | 474, 714, 1400 |
| PleI | GAGTC | 8 cut(s) | 559, 799, 1704, 2303, 2399, 2434, 2450, 2674 |
| PpsI | GAGTC | 8 cut(s) | 559, 799, 1704, 2303, 2399, 2434, 2450, 2674 |
| PpuMI | RGGWCCY | 3 cut(s) | 516, 756, 1180 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1923 |
| Psp124BI | GAGCTC | 1 cut(s) | 1505 |
| Psp5II | RGGWCCY | 3 cut(s) | 516, 756, 1180 |
| PspEI | GGTNACC | 1 cut(s) | 1270 |
| PspFI | CCCAGC | 1 cut(s) | 1029 |
| PspPI | GGNCC | 9 cut(s) | 516, 756, 894, 1180, 1701, 1771, 1918, 2012, 2121 |
| PspPPI | RGGWCCY | 3 cut(s) | 516, 756, 1180 |
| PstI | CTGCAG | 1 cut(s) | 2425 |
| PstNI | CAGNNNCTG | 3 cut(s) | 1099, 2083, 2429 |
| PsuI | RGATCY | 5 cut(s) | 286, 578, 818, 1907, 2134 |
| PvuII | CAGCTG | 3 cut(s) | 1132, 1396, 2080 |
| RruI | TCGCGA | 1 cut(s) | 170 |
| RseI | CAYNNNNRTG | 3 cut(s) | 926, 1637, 1823 |
| SacI | GAGCTC | 1 cut(s) | 1505 |
| SacII | CCGCGG | 1 cut(s) | 2481 |
| SalI | GTCGAC | 1 cut(s) | 1673 |
| SaqAI | TTAA | 3 cut(s) | 135, 2232, 2574 |
| Sau96I | GGNCC | 9 cut(s) | 516, 756, 894, 1180, 1701, 1771, 1918, 2012, 2121 |
| SchI | GAGTC | 8 cut(s) | 560, 800, 1705, 2303, 2399, 2435, 2450, 2675 |
| SduI | GDGCHC | 2 cut(s) | 1505, 2227 |
| SexAI | ACCWGGT | 1 cut(s) | 2157 |
| SfaNI | GCATC | 4 cut(s) | 106, 1098, 1645, 2208 |
| SfcI | CTRYAG | 2 cut(s) | 1091, 2421 |
| Sfr303I | CCGCGG | 1 cut(s) | 2481 |
| SgrBI | CCGCGG | 1 cut(s) | 2481 |
| SinI | GGWCC | 7 cut(s) | 516, 756, 894, 1180, 1701, 1918, 2121 |
| SmaI | CCCGGG | 5 cut(s) | 1026, 1469, 2004, 2207, 2402 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 926, 1637, 1823 |
| SmlI | CTYRAG | 2 cut(s) | 1243, 2357 |
| SmoI | CTYRAG | 2 cut(s) | 1243, 2357 |
| Sse9I | AATT | 9 cut(s) | 44, 131, 234, 390, 497, 737, 1141, 1803, 2612 |
| SspMI | CTAG | 5 cut(s) | 1284, 1301, 1355, 1541, 2600 |
| SstI | GAGCTC | 1 cut(s) | 1505 |
| StyI | CCWWGG | 6 cut(s) | 209, 226, 345, 1060, 1791, 2026 |
| TaaI | ACNGT | 9 cut(s) | 110, 152, 265, 388, 844, 898, 1095, 1270, 1745 |
| TaiI | ACGT | 2 cut(s) | 1803, 1949 |
| TaqII | GACCGA | 1 cut(s) | 1689 |
| TasI | AATT | 9 cut(s) | 44, 131, 234, 390, 497, 737, 1141, 1803, 2612 |
| TatI | WGTACW | 2 cut(s) | 1239, 2505 |
| TauI | GCSGC | 6 cut(s) | 952, 1283, 1300, 2333, 2591, 2660 |
| TfiI | GAWTC | 7 cut(s) | 401, 641, 1015, 1048, 1512, 2191, 2210 |
| Tru1I | TTAA | 3 cut(s) | 135, 2232, 2574 |
| Tru9I | TTAA | 3 cut(s) | 135, 2232, 2574 |
| TscAI | CASTG | 3 cut(s) | 849, 1957, 2260 |
| TseFI | GTSAC | 5 cut(s) | 598, 838, 1270, 1942, 2016 |
| Tsp45I | GTSAC | 5 cut(s) | 598, 838, 1270, 1942, 2016 |
| TspDTI | ATGAA | 5 cut(s) | 944, 1504, 2103, 2121, 2582 |
| TspMI | CCCGGG | 5 cut(s) | 1024, 1467, 2002, 2205, 2400 |
| TspRI | CASTG | 3 cut(s) | 849, 1957, 2260 |
| VpaK11BI | GGWCC | 7 cut(s) | 516, 756, 894, 1180, 1701, 1918, 2121 |
| XagI | CCTNNNNNAGG | 4 cut(s) | 448, 688, 2156, 2276 |
| XapI | RAATTY | 4 cut(s) | 44, 131, 1141, 2612 |
| XbaI | TCTAGA | 1 cut(s) | 2599 |
| XceI | RCATGY | 2 cut(s) | 949, 1636 |
| XmaI | CCCGGG | 5 cut(s) | 1024, 1467, 2002, 2205, 2400 |
| XmiI | GTMKAC | 3 cut(s) | 492, 732, 1674 |
| XspI | CTAG | 5 cut(s) | 1284, 1301, 1355, 1541, 2600 |