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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1496 |
| AarI | CACCTGC | 1 cut(s) | 1909 |
| Acc36I | ACCTGC | 1 cut(s) | 1909 |
| Acc65I | GGTACC | 3 cut(s) | 115, 434, 1214 |
| AccB1I | GGYRCC | 5 cut(s) | 115, 434, 897, 1214, 2330 |
| AccBSI | CCGCTC | 1 cut(s) | 1963 |
| AccI | GTMKAC | 2 cut(s) | 129, 1284 |
| AccII | CGCG | 7 cut(s) | 51, 61, 635, 785, 1034, 1454, 2009 |
| AclWI | GGATC | 5 cut(s) | 221, 1666, 1758, 2012, 2313 |
| AcoI | YGGCCR | 3 cut(s) | 657, 819, 1986 |
| AcsI | RAATTY | 3 cut(s) | 280, 629, 2270 |
| AcuI | CTGAAG | 3 cut(s) | 785, 1775, 1910 |
| AcvI | CACGTG | 1 cut(s) | 219 |
| AflIII | ACRYGT | 2 cut(s) | 216, 218 |
| AgeI | ACCGGT | 2 cut(s) | 525, 1211 |
| AgsI | TTSAA | 4 cut(s) | 280, 1400, 1849, 2233 |
| AhlI | ACTAGT | 2 cut(s) | 1111, 1385 |
| AjiI | CACGTC | 2 cut(s) | 45, 1468 |
| AjnI | CCWGG | 6 cut(s) | 461, 621, 835, 1582, 1800, 2118 |
| AleI | CACNNNNGTG | 1 cut(s) | 315 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 551, 583 |
| Alw21I | GWGCWC | 2 cut(s) | 1316, 2184 |
| Alw26I | GTCTC | 3 cut(s) | 721, 1474, 2098 |
| AlwI | GGATC | 5 cut(s) | 221, 1666, 1758, 2012, 2313 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 910, 1699 |
| Ama87I | CYCGRG | 8 cut(s) | 537, 665, 1278, 1370, 1618, 1821, 2037, 2058 |
| ApoI | RAATTY | 3 cut(s) | 280, 629, 2270 |
| AsiGI | ACCGGT | 2 cut(s) | 525, 1211 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 863, 1407 |
| Asp718I | GGTACC | 3 cut(s) | 115, 434, 1214 |
| AspLEI | GCGC | 4 cut(s) | 51, 61, 850, 1456 |
| AsuHPI | GGTGA | 7 cut(s) | 626, 638, 1411, 1448, 1626, 1681, 1933 |
| AvaI | CYCGRG | 8 cut(s) | 537, 665, 1278, 1370, 1618, 1821, 2037, 2058 |
| AvaII | GGWCC | 6 cut(s) | 427, 567, 705, 1234, 1622, 1737 |
| BaeGI | GKGCMC | 1 cut(s) | 1843 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 426, 459 |
| BalI | TGGCCA | 1 cut(s) | 659 |
| BanI | GGYRCC | 5 cut(s) | 115, 434, 897, 1214, 2330 |
| BanII | GRGCYC | 2 cut(s) | 1316, 2184 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 671, 703 |
| BauI | CACGAG | 2 cut(s) | 854, 2147 |
| BbrPI | CACGTG | 1 cut(s) | 219 |
| BbsI | GAAGAC | 2 cut(s) | 341, 486 |
| Bbv12I | GWGCWC | 2 cut(s) | 1316, 2184 |
| BbvCI | CCTCAGC | 3 cut(s) | 237, 510, 2031 |
| BccI | CCATC | 7 cut(s) | 442, 695, 803, 811, 973, 1510, 1694 |
| BceAI | ACGGC | 5 cut(s) | 273, 1274, 1797, 2075, 2138 |
| BciT130I | CCWGG | 6 cut(s) | 463, 623, 837, 1584, 1802, 2120 |
| BciVI | GTATCC | 3 cut(s) | 466, 768, 1873 |
| BcoDI | GTCTC | 3 cut(s) | 721, 1474, 2098 |
| BcuI | ACTAGT | 2 cut(s) | 1111, 1385 |
| BfaI | CTAG | 7 cut(s) | 576, 1095, 1112, 1166, 1352, 1358, 1386 |
| BfmI | CTRYAG | 1 cut(s) | 902 |
| BfoI | RGCGCY | 1 cut(s) | 851 |
| BfuAI | ACCTGC | 1 cut(s) | 1909 |
| BfuI | GTATCC | 3 cut(s) | 466, 768, 1873 |
| BlpI | GCTNAGC | 1 cut(s) | 1995 |
| Bme18I | GGWCC | 6 cut(s) | 427, 567, 705, 1234, 1622, 1737 |
| BmeT110I | CYCGRG | 8 cut(s) | 537, 665, 1278, 1370, 1618, 1821, 2037, 2058 |
| BmgBI | CACGTC | 2 cut(s) | 45, 1468 |
| BmrI | ACTGGG | 2 cut(s) | 158, 1569 |
| BmsI | GCATC | 6 cut(s) | 22, 255, 360, 909, 1824, 1855 |
| BmuI | ACTGGG | 2 cut(s) | 158, 1569 |
| BoxI | GACNNNNGTC | 2 cut(s) | 1466, 1627 |
| BpiI | GAAGAC | 2 cut(s) | 341, 486 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 1298, 1330, 2171, 2203 |
| BpmI | CTGGAG | 5 cut(s) | 96, 1433, 1973, 2141, 2309 |
| Bpu10I | CCTNAGC | 3 cut(s) | 237, 510, 2031 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1995 |
| BpuEI | CTTGAG | 2 cut(s) | 1039, 2036 |
| BsaAI | YACGTR | 1 cut(s) | 219 |
| BsaBI | GATNNNNATC | 2 cut(s) | 350, 1884 |
| BsaWI | WCCGGW | 4 cut(s) | 112, 525, 1211, 1666 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 2153, 2183 |
| Bse118I | RCCGGY | 4 cut(s) | 525, 1211, 1528, 2333 |
| Bse1I | ACTGG | 5 cut(s) | 153, 490, 1237, 1564, 1594 |
| Bse8I | GATNNNNATC | 2 cut(s) | 350, 1884 |
| BseBI | CCWGG | 6 cut(s) | 463, 623, 837, 1584, 1802, 2120 |
| BseGI | GGATG | 6 cut(s) | 453, 706, 814, 924, 1326, 1521 |
| BseJI | GATNNNNATC | 2 cut(s) | 350, 1884 |
| BseMII | CTCAG | 5 cut(s) | 228, 344, 501, 1691, 2022 |
| BseNI | ACTGG | 5 cut(s) | 153, 490, 1237, 1564, 1594 |
| BseRI | GAGGAG | 5 cut(s) | 371, 1324, 1537, 1889, 1910 |
| BseSI | GKGCMC | 1 cut(s) | 1843 |
| BseYI | CCCAGC | 2 cut(s) | 13, 1169 |
| BsgI | GTGCAG | 1 cut(s) | 836 |
| Bsh1236I | CGCG | 7 cut(s) | 51, 61, 635, 785, 1034, 1454, 2009 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1774 |
| BshNI | GGYRCC | 5 cut(s) | 115, 434, 897, 1214, 2330 |
| BshTI | ACCGGT | 2 cut(s) | 525, 1211 |
| BsiEI | CGRYCG | 1 cut(s) | 1774 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1316, 2184 |
| BsiHKCI | CYCGRG | 8 cut(s) | 537, 665, 1278, 1370, 1618, 1821, 2037, 2058 |
| BslFI | GGGAC | 8 cut(s) | 156, 413, 718, 1004, 1247, 1635, 1979, 2075 |
| BsmAI | GTCTC | 3 cut(s) | 721, 1474, 2098 |
| BsmBI | CGTCTC | 3 cut(s) | 721, 1474, 2098 |
| BsmFI | GGGAC | 8 cut(s) | 156, 413, 718, 1004, 1247, 1635, 1979, 2075 |
| BsmI | GAATGC | 2 cut(s) | 211, 672 |
| BsoBI | CYCGRG | 8 cut(s) | 537, 665, 1278, 1370, 1618, 1821, 2037, 2058 |
| Bsp1286I | GDGCHC | 3 cut(s) | 1316, 1843, 2184 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1995 |
| Bsp19I | CCATGG | 1 cut(s) | 1482 |
| BspCNI | CTCAG | 5 cut(s) | 229, 343, 502, 1692, 2023 |
| BspFNI | CGCG | 7 cut(s) | 51, 61, 635, 785, 1034, 1454, 2009 |
| BspHI | TCATGA | 1 cut(s) | 2223 |
| BspMI | ACCTGC | 1 cut(s) | 1909 |
| BspPI | GGATC | 5 cut(s) | 221, 1666, 1758, 2012, 2313 |
| BspQI | GCTCTTC | 1 cut(s) | 2172 |
| BspT107I | GGYRCC | 5 cut(s) | 115, 434, 897, 1214, 2330 |
| BsrBI | CCGCTC | 1 cut(s) | 1963 |
| BsrFI | RCCGGY | 4 cut(s) | 525, 1211, 1528, 2333 |
| BsrI | ACTGG | 5 cut(s) | 153, 490, 1237, 1564, 1594 |
| BssAI | RCCGGY | 4 cut(s) | 525, 1211, 1528, 2333 |
| BssNAI | GTATAC | 1 cut(s) | 1285 |
| BssSI | CACGAG | 2 cut(s) | 854, 2147 |
| BssT1I | CCWWGG | 5 cut(s) | 184, 372, 871, 1482, 1785 |
| Bst1107I | GTATAC | 1 cut(s) | 1285 |
| Bst2BI | CACGAG | 2 cut(s) | 854, 2147 |
| Bst2UI | CCWGG | 6 cut(s) | 463, 623, 837, 1584, 1802, 2120 |
| Bst4CI | ACNGT | 8 cut(s) | 301, 439, 655, 709, 906, 974, 1081, 1344 |
| Bst6I | CTCTTC | 5 cut(s) | 1262, 1592, 1850, 2037, 2172 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 830, 2087 |
| BstBAI | YACGTR | 1 cut(s) | 219 |
| BstC8I | GCNNGC | 4 cut(s) | 1839, 1870, 1986, 2196 |
| BstDEI | CTNAG | 9 cut(s) | 237, 330, 510, 532, 794, 803, 1700, 1995, 2031 |
| BstDSI | CCRYGG | 2 cut(s) | 1482, 2136 |
| BstEII | GGTNACC | 1 cut(s) | 1081 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 574, 834 |
| BstF5I | GGATG | 6 cut(s) | 453, 706, 814, 924, 1326, 1521 |
| BstFNI | CGCG | 7 cut(s) | 51, 61, 635, 785, 1034, 1454, 2009 |
| BstH2I | RGCGCY | 1 cut(s) | 851 |
| BstHHI | GCGC | 4 cut(s) | 51, 61, 850, 1456 |
| BstMAI | GTCTC | 3 cut(s) | 721, 1474, 2098 |
| BstMCI | CGRYCG | 1 cut(s) | 1774 |
| BstNI | CCWGG | 6 cut(s) | 463, 623, 837, 1584, 1802, 2120 |
| BstNSI | RCATGY | 1 cut(s) | 760 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 1466, 1627 |
| BstPI | GGTNACC | 1 cut(s) | 1081 |
| BstSFI | CTRYAG | 1 cut(s) | 902 |
| BstSLI | GKGCMC | 1 cut(s) | 1843 |
| BstUI | CGCG | 7 cut(s) | 51, 61, 635, 785, 1034, 1454, 2009 |
| BstV2I | GAAGAC | 2 cut(s) | 341, 486 |
| BstX2I | RGATCY | 1 cut(s) | 1750 |
| BstYI | RGATCY | 1 cut(s) | 1750 |
| BstZ17I | GTATAC | 1 cut(s) | 1285 |
| BsuI | GTATCC | 3 cut(s) | 466, 768, 1873 |
| BtgI | CCRYGG | 2 cut(s) | 1482, 2136 |
| BtgZI | GCGATG | 1 cut(s) | 1581 |
| BtrI | CACGTC | 2 cut(s) | 45, 1468 |
| BtsCI | GGATG | 6 cut(s) | 453, 706, 814, 924, 1326, 1521 |
| BtsIMutI | CAGTG | 2 cut(s) | 660, 1557 |
| BveI | ACCTGC | 1 cut(s) | 1909 |
| Cac8I | GCNNGC | 4 cut(s) | 1839, 1870, 1986, 2196 |
| CaiI | CAGNNNCTG | 2 cut(s) | 910, 1699 |
| CciI | TCATGA | 1 cut(s) | 2223 |
| CfoI | GCGC | 4 cut(s) | 51, 61, 850, 1456 |
| Cfr10I | RCCGGY | 4 cut(s) | 525, 1211, 1528, 2333 |
| Cfr9I | CCCGGG | 5 cut(s) | 537, 1278, 1370, 2037, 2058 |
| CseI | GACGC | 3 cut(s) | 50, 1460, 1976 |
| CspAI | ACCGGT | 2 cut(s) | 525, 1211 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 989, 1024 |
| CviAII | CATG | 6 cut(s) | 757, 1483, 1741, 1934, 2097, 2224 |
| DdeI | CTNAG | 9 cut(s) | 237, 330, 510, 532, 794, 803, 1700, 1995, 2031 |
| DraI | TTTAAA | 1 cut(s) | 138 |
| EaeI | YGGCCR | 3 cut(s) | 657, 819, 1986 |
| Eam1104I | CTCTTC | 5 cut(s) | 1262, 1592, 1850, 2037, 2172 |
| EarI | CTCTTC | 5 cut(s) | 1262, 1592, 1850, 2037, 2172 |
| EciI | GGCGGA | 1 cut(s) | 1994 |
| Ecl136II | GAGCTC | 2 cut(s) | 1314, 2182 |
| Eco130I | CCWWGG | 5 cut(s) | 184, 372, 871, 1482, 1785 |
| Eco147I | AGGCCT | 1 cut(s) | 799 |
| Eco24I | GRGCYC | 2 cut(s) | 1316, 2184 |
| Eco32I | GATATC | 1 cut(s) | 1762 |
| Eco47I | GGWCC | 6 cut(s) | 427, 567, 705, 1234, 1622, 1737 |
| Eco53kI | GAGCTC | 2 cut(s) | 1314, 2182 |
| Eco57I | CTGAAG | 3 cut(s) | 785, 1775, 1910 |
| Eco72I | CACGTG | 1 cut(s) | 219 |
| Eco88I | CYCGRG | 8 cut(s) | 537, 665, 1278, 1370, 1618, 1821, 2037, 2058 |
| Eco91I | GGTNACC | 1 cut(s) | 1081 |
| EcoICRI | GAGCTC | 2 cut(s) | 1314, 2182 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 574, 834 |
| EcoO109I | RGGNCCY | 3 cut(s) | 427, 567, 640 |
| EcoO65I | GGTNACC | 1 cut(s) | 1081 |
| EcoRI | GAATTC | 1 cut(s) | 629 |
| EcoRII | CCWGG | 6 cut(s) | 461, 621, 835, 1582, 1800, 2118 |
| EcoRV | GATATC | 1 cut(s) | 1762 |
| EcoT14I | CCWWGG | 5 cut(s) | 184, 372, 871, 1482, 1785 |
| EcoT22I | ATGCAT | 1 cut(s) | 270 |
| EcoT38I | GRGCYC | 2 cut(s) | 1316, 2184 |
| ErhI | CCWWGG | 5 cut(s) | 184, 372, 871, 1482, 1785 |
| Esp3I | CGTCTC | 3 cut(s) | 721, 1474, 2098 |
| FaeI | CATG | 6 cut(s) | 760, 1486, 1744, 1937, 2100, 2227 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1392, 1424 |
| FaqI | GGGAC | 8 cut(s) | 156, 413, 718, 1004, 1247, 1635, 1979, 2075 |
| FatI | CATG | 6 cut(s) | 756, 1482, 1740, 1933, 2096, 2223 |
| FauI | CCCGC | 6 cut(s) | 1066, 1666, 1830, 1956, 2002, 2271 |
| FblI | GTMKAC | 2 cut(s) | 129, 1284 |
| FokI | GGATG | 6 cut(s) | 460, 713, 821, 931, 1313, 1528 |
| FriOI | GRGCYC | 2 cut(s) | 1316, 2184 |
| FspBI | CTAG | 7 cut(s) | 576, 1095, 1112, 1166, 1352, 1358, 1386 |
| GlaI | GCGC | 4 cut(s) | 50, 60, 849, 1455 |
| GsaI | CCCAGC | 2 cut(s) | 17, 1173 |
| GsuI | CTGGAG | 5 cut(s) | 96, 1433, 1973, 2141, 2309 |
| HaeII | RGCGCY | 1 cut(s) | 851 |
| HgaI | GACGC | 3 cut(s) | 50, 1460, 1976 |
| HhaI | GCGC | 4 cut(s) | 51, 61, 850, 1456 |
| Hin1II | CATG | 6 cut(s) | 760, 1486, 1744, 1937, 2100, 2227 |
| Hin6I | GCGC | 4 cut(s) | 49, 59, 848, 1454 |
| HinP1I | GCGC | 4 cut(s) | 49, 59, 848, 1454 |
| HinfI | GANTC | 8 cut(s) | 452, 602, 859, 1323, 1628, 1807, 1826, 2063 |
| HphI | GGTGA | 7 cut(s) | 626, 638, 1411, 1448, 1626, 1681, 1933 |
| Hpy166II | GTNNAC | 6 cut(s) | 130, 690, 1039, 1285, 1439, 1855 |
| Hpy8I | GTNNAC | 6 cut(s) | 130, 690, 1039, 1285, 1439, 1855 |
| Hpy99I | CGWCG | 6 cut(s) | 66, 259, 611, 719, 1427, 1454 |
| HpyCH4III | ACNGT | 8 cut(s) | 301, 439, 655, 709, 906, 974, 1081, 1344 |
| HpyCH4IV | ACGT | 4 cut(s) | 44, 218, 730, 1467 |
| HpyCH4V | TGCA | 5 cut(s) | 158, 268, 351, 853, 2081 |
| HpyF3I | CTNAG | 9 cut(s) | 237, 330, 510, 532, 794, 803, 1700, 1995, 2031 |
| HpySE526I | ACGT | 4 cut(s) | 44, 218, 730, 1467 |
| Hsp92II | CATG | 6 cut(s) | 760, 1486, 1744, 1937, 2100, 2227 |
| HspAI | GCGC | 4 cut(s) | 49, 59, 848, 1454 |
| KpnI | GGTACC | 3 cut(s) | 119, 438, 1218 |
| LguI | GCTCTTC | 1 cut(s) | 2172 |
| LmnI | GCTCC | 5 cut(s) | 1311, 1371, 1563, 1960, 1981 |
| LweI | GCATC | 6 cut(s) | 22, 255, 360, 909, 1824, 1855 |
| MaeI | CTAG | 7 cut(s) | 576, 1095, 1112, 1166, 1352, 1358, 1386 |
| MaeII | ACGT | 4 cut(s) | 44, 218, 730, 1467 |
| MaeIII | GTNAC | 6 cut(s) | 649, 930, 1081, 1114, 1459, 1632 |
| MbiI | CCGCTC | 1 cut(s) | 1963 |
| MfeI | CAATTG | 1 cut(s) | 1395 |
| MflI | RGATCY | 1 cut(s) | 1750 |
| MhlI | GDGCHC | 3 cut(s) | 1316, 1843, 2184 |
| MlsI | TGGCCA | 1 cut(s) | 659 |
| MluCI | AATT | 6 cut(s) | 280, 548, 629, 863, 1395, 2270 |
| MluNI | TGGCCA | 1 cut(s) | 659 |
| MlyI | GAGTC | 3 cut(s) | 611, 1637, 2057 |
| MmeI | TCCRAC | 2 cut(s) | 169, 630 |
| Mox20I | TGGCCA | 1 cut(s) | 659 |
| Mph1103I | ATGCAT | 1 cut(s) | 270 |
| MroXI | GAANNNNTTC | 2 cut(s) | 863, 1407 |
| MscI | TGGCCA | 1 cut(s) | 659 |
| MseI | TTAA | 3 cut(s) | 137, 284, 1188 |
| MslI | CAYNNNNRTG | 5 cut(s) | 315, 755, 1514, 1667, 2189 |
| Msp20I | TGGCCA | 1 cut(s) | 659 |
| MspA1I | CMGCKG | 4 cut(s) | 889, 943, 1207, 2002 |
| MunI | CAATTG | 1 cut(s) | 1395 |
| Mva1269I | GAATGC | 2 cut(s) | 211, 672 |
| MvaI | CCWGG | 6 cut(s) | 463, 623, 837, 1584, 1802, 2120 |
| MvnI | CGCG | 7 cut(s) | 51, 61, 635, 785, 1034, 1454, 2009 |
| NcoI | CCATGG | 1 cut(s) | 1482 |
| NlaIII | CATG | 6 cut(s) | 760, 1486, 1744, 1937, 2100, 2227 |
| NmeAIII | GCCGAG | 1 cut(s) | 708 |
| NmuCI | GTSAC | 3 cut(s) | 649, 1459, 1632 |
| NsiI | ATGCAT | 1 cut(s) | 270 |
| NspI | RCATGY | 1 cut(s) | 760 |
| OliI | CACNNNNGTG | 1 cut(s) | 315 |
| PagI | TCATGA | 1 cut(s) | 2223 |
| PaqCI | CACCTGC | 1 cut(s) | 1909 |
| PasI | CCCWGGG | 1 cut(s) | 836 |
| PceI | AGGCCT | 1 cut(s) | 799 |
| PciSI | GCTCTTC | 1 cut(s) | 2172 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 482, 569, 1716 |
| PctI | GAATGC | 2 cut(s) | 211, 672 |
| PdmI | GAANNNNTTC | 2 cut(s) | 863, 1407 |
| PfeI | GAWTC | 5 cut(s) | 452, 859, 1323, 1807, 1826 |
| PflFI | GACNNNGTC | 1 cut(s) | 2323 |
| PinAI | ACCGGT | 2 cut(s) | 525, 1211 |
| PleI | GAGTC | 3 cut(s) | 610, 1636, 2057 |
| PmaCI | CACGTG | 1 cut(s) | 219 |
| PmlI | CACGTG | 1 cut(s) | 219 |
| PpsI | GAGTC | 3 cut(s) | 610, 1636, 2057 |
| Ppu21I | YACGTR | 1 cut(s) | 219 |
| PpuMI | RGGWCCY | 2 cut(s) | 427, 567 |
| PshAI | GACNNNNGTC | 2 cut(s) | 1466, 1627 |
| PsiI | TTATAA | 1 cut(s) | 1496 |
| Psp124BI | GAGCTC | 2 cut(s) | 1316, 2184 |
| Psp5II | RGGWCCY | 2 cut(s) | 427, 567 |
| Psp6I | CCWGG | 6 cut(s) | 461, 621, 835, 1582, 1800, 2118 |
| PspCI | CACGTG | 1 cut(s) | 219 |
| PspEI | GGTNACC | 1 cut(s) | 1081 |
| PspFI | CCCAGC | 2 cut(s) | 13, 1169 |
| PspGI | CCWGG | 6 cut(s) | 461, 621, 835, 1582, 1800, 2118 |
| PspPPI | RGGWCCY | 2 cut(s) | 427, 567 |
| PstNI | CAGNNNCTG | 2 cut(s) | 910, 1699 |
| PsuI | RGATCY | 1 cut(s) | 1750 |
| PsyI | GACNNNGTC | 1 cut(s) | 2323 |
| PvuII | CAGCTG | 2 cut(s) | 943, 1207 |
| RseI | CAYNNNNRTG | 5 cut(s) | 315, 755, 1514, 1667, 2189 |
| SacI | GAGCTC | 2 cut(s) | 1316, 2184 |
| SapI | GCTCTTC | 1 cut(s) | 2172 |
| SaqAI | TTAA | 3 cut(s) | 137, 284, 1188 |
| SchI | GAGTC | 3 cut(s) | 611, 1637, 2057 |
| SduI | GDGCHC | 3 cut(s) | 1316, 1843, 2184 |
| SfaNI | GCATC | 6 cut(s) | 22, 255, 360, 909, 1824, 1855 |
| SfcI | CTRYAG | 1 cut(s) | 902 |
| SinI | GGWCC | 6 cut(s) | 427, 567, 705, 1234, 1622, 1737 |
| SmaI | CCCGGG | 5 cut(s) | 539, 1280, 1372, 2039, 2060 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 315, 755, 1514, 1667, 2189 |
| SmlI | CTYRAG | 2 cut(s) | 1054, 2015 |
| SmoI | CTYRAG | 2 cut(s) | 1054, 2015 |
| SpeI | ACTAGT | 2 cut(s) | 1111, 1385 |
| Sse9I | AATT | 6 cut(s) | 280, 548, 629, 863, 1395, 2270 |
| SseBI | AGGCCT | 1 cut(s) | 799 |
| SspMI | CTAG | 7 cut(s) | 576, 1095, 1112, 1166, 1352, 1358, 1386 |
| SstI | GAGCTC | 2 cut(s) | 1316, 2184 |
| StuI | AGGCCT | 1 cut(s) | 799 |
| StyI | CCWWGG | 5 cut(s) | 184, 372, 871, 1482, 1785 |
| TaaI | ACNGT | 8 cut(s) | 301, 439, 655, 709, 906, 974, 1081, 1344 |
| TaiI | ACGT | 4 cut(s) | 47, 221, 733, 1470 |
| TaqI | TCGA | 9 cut(s) | 192, 254, 354, 572, 753, 1133, 1376, 1764, 1770 |
| TaqII | GACCGA | 2 cut(s) | 1380, 1788 |
| TasI | AATT | 6 cut(s) | 280, 548, 629, 863, 1395, 2270 |
| TatI | WGTACW | 1 cut(s) | 2163 |
| TauI | GCSGC | 6 cut(s) | 59, 824, 1094, 1991, 1994, 2318 |
| TfiI | GAWTC | 5 cut(s) | 452, 859, 1323, 1807, 1826 |
| Tru1I | TTAA | 3 cut(s) | 137, 284, 1188 |
| Tru9I | TTAA | 3 cut(s) | 137, 284, 1188 |
| TscAI | CASTG | 2 cut(s) | 660, 1564 |
| TseFI | GTSAC | 3 cut(s) | 649, 1459, 1632 |
| Tsp45I | GTSAC | 3 cut(s) | 649, 1459, 1632 |
| TspDTI | ATGAA | 6 cut(s) | 755, 1315, 1737, 1922, 2240, 2277 |
| TspGWI | ACGGA | 1 cut(s) | 1006 |
| TspMI | CCCGGG | 5 cut(s) | 537, 1278, 1370, 2037, 2058 |
| TspRI | CASTG | 2 cut(s) | 660, 1564 |
| Tth111I | GACNNNGTC | 1 cut(s) | 2323 |
| VpaK11BI | GGWCC | 6 cut(s) | 427, 567, 705, 1234, 1622, 1737 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 574, 834 |
| XapI | RAATTY | 3 cut(s) | 280, 629, 2270 |
| XceI | RCATGY | 1 cut(s) | 760 |
| XmaI | CCCGGG | 5 cut(s) | 537, 1278, 1370, 2037, 2058 |
| XmiI | GTMKAC | 2 cut(s) | 129, 1284 |
| XmnI | GAANNNNTTC | 2 cut(s) | 863, 1407 |
| XspI | CTAG | 7 cut(s) | 576, 1095, 1112, 1166, 1352, 1358, 1386 |
| Zsp2I | ATGCAT | 1 cut(s) | 270 |