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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 2 cut(s) | 1630, 1848 |
| Acc36I | ACCTGC | 2 cut(s) | 1630, 1848 |
| Acc65I | GGTACC | 8 cut(s) | 19, 85, 151, 217, 283, 424, 458, 494 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 1099, 1557 |
| AccBSI | CCGCTC | 2 cut(s) | 1684, 2083 |
| AccI | GTMKAC | 3 cut(s) | 632, 749, 1146 |
| AccII | CGCG | 4 cut(s) | 884, 1730, 1759, 1859 |
| AclWI | GGATC | 5 cut(s) | 547, 697, 1220, 1733, 1854 |
| AcoI | YGGCCR | 3 cut(s) | 879, 1707, 1968 |
| AcsI | RAATTY | 7 cut(s) | 71, 137, 203, 269, 344, 410, 1991 |
| AcuI | CTGAAG | 4 cut(s) | 591, 845, 1496, 1631 |
| AcyI | GRCGYC | 1 cut(s) | 1128 |
| AdeI | CACNNNGTG | 1 cut(s) | 1252 |
| AgeI | ACCGGT | 1 cut(s) | 2072 |
| AgsI | TTSAA | 4 cut(s) | 1181, 1570, 1696, 1954 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1153 |
| AjiI | CACGTC | 1 cut(s) | 1249 |
| AjnI | CCWGG | 4 cut(s) | 15, 521, 1116, 2007 |
| Alw21I | GWGCWC | 1 cut(s) | 1905 |
| Alw26I | GTCTC | 4 cut(s) | 84, 1380, 1753, 2009 |
| AlwI | GGATC | 5 cut(s) | 547, 697, 1220, 1733, 1854 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 970 |
| Ama87I | CYCGRG | 7 cut(s) | 702, 725, 782, 895, 1339, 1542, 1779 |
| ApeKI | GCWGC | 8 cut(s) | 910, 949, 973, 1000, 1255, 1414, 1720, 1922 |
| ApoI | RAATTY | 7 cut(s) | 71, 137, 203, 269, 344, 410, 1991 |
| AsiGI | ACCGGT | 1 cut(s) | 2072 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1188 |
| Asp718I | GGTACC | 8 cut(s) | 19, 85, 151, 217, 283, 424, 458, 494 |
| AspA2I | CCTAGG | 1 cut(s) | 1150 |
| AspLEI | GCGC | 2 cut(s) | 910, 1759 |
| AspS9I | GGNCC | 7 cut(s) | 487, 700, 1114, 1343, 1458, 1503, 2061 |
| AsuHPI | GGTGA | 9 cut(s) | 686, 984, 1192, 1229, 1328, 1481, 1654, 1754, 1780 |
| AvaI | CYCGRG | 7 cut(s) | 702, 725, 782, 895, 1339, 1542, 1779 |
| AvaII | GGWCC | 4 cut(s) | 487, 1343, 1458, 2061 |
| AvrII | CCTAGG | 1 cut(s) | 1150 |
| BaeGI | GKGCMC | 1 cut(s) | 1564 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 486, 519 |
| BanII | GRGCYC | 1 cut(s) | 1905 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1116, 1148 |
| BauI | CACGAG | 1 cut(s) | 914 |
| BbsI | GAAGAC | 2 cut(s) | 546, 1131 |
| Bbv12I | GWGCWC | 1 cut(s) | 1905 |
| BbvI | GCAGC | 8 cut(s) | 897, 936, 960, 1012, 1267, 1426, 1732, 1909 |
| BceAI | ACGGC | 4 cut(s) | 1518, 1694, 1796, 1859 |
| BciT130I | CCWGG | 4 cut(s) | 17, 523, 1118, 2009 |
| BciVI | GTATCC | 2 cut(s) | 526, 1967 |
| BcoDI | GTCTC | 4 cut(s) | 84, 1380, 1753, 2009 |
| BfaI | CTAG | 2 cut(s) | 242, 1151 |
| BfmI | CTRYAG | 3 cut(s) | 962, 1800, 1841 |
| BfoI | RGCGCY | 1 cut(s) | 911 |
| BfuAI | ACCTGC | 2 cut(s) | 1630, 1848 |
| BfuI | GTATCC | 2 cut(s) | 526, 1967 |
| BglII | AGATCT | 1 cut(s) | 2026 |
| BlnI | CCTAGG | 1 cut(s) | 1150 |
| BlpI | GCTNAGC | 2 cut(s) | 383, 1716 |
| Bme18I | GGWCC | 4 cut(s) | 487, 1343, 1458, 2061 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1153 |
| BmeT110I | CYCGRG | 7 cut(s) | 702, 725, 782, 895, 1339, 1542, 1779 |
| BmgBI | CACGTC | 1 cut(s) | 1249 |
| BmgT120I | GGNCC | 7 cut(s) | 487, 700, 1114, 1343, 1458, 1503, 2061 |
| BmrI | ACTGGG | 2 cut(s) | 645, 1087 |
| BmsI | GCATC | 3 cut(s) | 832, 969, 1078 |
| BmuI | ACTGGG | 2 cut(s) | 645, 1087 |
| BoxI | GACNNNNGTC | 2 cut(s) | 1247, 1348 |
| BpiI | GAAGAC | 2 cut(s) | 546, 1131 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1673, 1705 |
| BpmI | CTGGAG | 4 cut(s) | 339, 1214, 1694, 2051 |
| Bpu10I | CCTNAGC | 1 cut(s) | 854 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 383, 1716 |
| BpuEI | CTTGAG | 1 cut(s) | 1757 |
| Bsa29I | ATCGAT | 3 cut(s) | 582, 813, 1140 |
| BsaBI | GATNNNNATC | 2 cut(s) | 582, 1605 |
| BsaHI | GRCGYC | 1 cut(s) | 1128 |
| BsaI | GGTCTC | 1 cut(s) | 2009 |
| BsaWI | WCCGGW | 8 cut(s) | 22, 82, 148, 214, 280, 421, 838, 2072 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1874, 1877, 1904, 1907 |
| Bse118I | RCCGGY | 1 cut(s) | 2072 |
| Bse1I | ACTGG | 5 cut(s) | 356, 550, 640, 1093, 2055 |
| Bse8I | GATNNNNATC | 2 cut(s) | 582, 1605 |
| BseBI | CCWGG | 4 cut(s) | 17, 523, 1118, 2009 |
| BseCI | ATCGAT | 3 cut(s) | 582, 813, 1140 |
| BseGI | GGATG | 9 cut(s) | 31, 513, 766, 847, 874, 984, 1090, 1302, 1987 |
| BseJI | GATNNNNATC | 2 cut(s) | 582, 1605 |
| BseMII | CTCAG | 3 cut(s) | 877, 1692, 1743 |
| BseNI | ACTGG | 5 cut(s) | 356, 550, 640, 1093, 2055 |
| BseRI | GAGGAG | 3 cut(s) | 674, 1631, 1895 |
| BseSI | GKGCMC | 1 cut(s) | 1564 |
| BseX3I | CGGCCG | 1 cut(s) | 1968 |
| BseXI | GCAGC | 8 cut(s) | 897, 936, 960, 1012, 1267, 1426, 1732, 1909 |
| BsgI | GTGCAG | 2 cut(s) | 896, 1274 |
| Bsh1236I | CGCG | 4 cut(s) | 884, 1730, 1759, 1859 |
| Bsh1285I | CGRYCG | 3 cut(s) | 586, 1495, 1971 |
| BshTI | ACCGGT | 1 cut(s) | 2072 |
| BshVI | ATCGAT | 3 cut(s) | 582, 813, 1140 |
| BsiEI | CGRYCG | 3 cut(s) | 586, 1495, 1971 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1905 |
| BsiHKCI | CYCGRG | 7 cut(s) | 702, 725, 782, 895, 1339, 1542, 1779 |
| BslFI | GGGAC | 6 cut(s) | 473, 778, 1356, 1700, 1796, 2047 |
| BsmAI | GTCTC | 4 cut(s) | 84, 1380, 1753, 2009 |
| BsmFI | GGGAC | 6 cut(s) | 473, 778, 1356, 1700, 1796, 2047 |
| BsmI | GAATGC | 1 cut(s) | 1018 |
| Bso31I | GGTCTC | 1 cut(s) | 2009 |
| BsoBI | CYCGRG | 7 cut(s) | 702, 725, 782, 895, 1339, 1542, 1779 |
| Bsp1286I | GDGCHC | 2 cut(s) | 1564, 1905 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 383, 1716 |
| BspCNI | CTCAG | 3 cut(s) | 876, 1693, 1744 |
| BspDI | ATCGAT | 3 cut(s) | 582, 813, 1140 |
| BspFNI | CGCG | 4 cut(s) | 884, 1730, 1759, 1859 |
| BspHI | TCATGA | 1 cut(s) | 1944 |
| BspMAI | CTGCAG | 1 cut(s) | 1845 |
| BspMI | ACCTGC | 2 cut(s) | 1630, 1848 |
| BspPI | GGATC | 5 cut(s) | 547, 697, 1220, 1733, 1854 |
| BspQI | GCTCTTC | 1 cut(s) | 1893 |
| BspTNI | GGTCTC | 1 cut(s) | 2009 |
| BsrBI | CCGCTC | 2 cut(s) | 1684, 2083 |
| BsrFI | RCCGGY | 1 cut(s) | 2072 |
| BsrI | ACTGG | 5 cut(s) | 356, 550, 640, 1093, 2055 |
| BssAI | RCCGGY | 1 cut(s) | 2072 |
| BssNAI | GTATAC | 1 cut(s) | 750 |
| BssNI | GRCGYC | 1 cut(s) | 1128 |
| BssSI | CACGAG | 1 cut(s) | 914 |
| BssT1I | CCWWGG | 3 cut(s) | 931, 1150, 1506 |
| Bst1107I | GTATAC | 1 cut(s) | 750 |
| Bst2BI | CACGAG | 1 cut(s) | 914 |
| Bst2UI | CCWGG | 4 cut(s) | 17, 523, 1118, 2009 |
| Bst4CI | ACNGT | 3 cut(s) | 499, 769, 966 |
| Bst6I | CTCTTC | 6 cut(s) | 1040, 1065, 1313, 1571, 1758, 1893 |
| BstACI | GRCGYC | 1 cut(s) | 1128 |
| BstC8I | GCNNGC | 4 cut(s) | 46, 567, 734, 2081 |
| BstDEI | CTNAG | 7 cut(s) | 383, 592, 854, 863, 1701, 1716, 1752 |
| BstDSI | CCRYGG | 2 cut(s) | 1857, 1875 |
| BstEII | GGTNACC | 1 cut(s) | 1469 |
| BstF5I | GGATG | 9 cut(s) | 31, 513, 766, 847, 874, 984, 1090, 1302, 1987 |
| BstFNI | CGCG | 4 cut(s) | 884, 1730, 1759, 1859 |
| BstH2I | RGCGCY | 1 cut(s) | 911 |
| BstHHI | GCGC | 2 cut(s) | 910, 1759 |
| BstMAI | GTCTC | 4 cut(s) | 84, 1380, 1753, 2009 |
| BstMCI | CGRYCG | 3 cut(s) | 586, 1495, 1971 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 45, 566, 890, 979, 1411, 1462, 1706 |
| BstNI | CCWGG | 4 cut(s) | 17, 523, 1118, 2009 |
| BstNSI | RCATGY | 1 cut(s) | 736 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 1247, 1348 |
| BstPI | GGTNACC | 1 cut(s) | 1469 |
| BstSFI | CTRYAG | 3 cut(s) | 962, 1800, 1841 |
| BstSLI | GKGCMC | 1 cut(s) | 1564 |
| BstUI | CGCG | 4 cut(s) | 884, 1730, 1759, 1859 |
| BstV1I | GCAGC | 8 cut(s) | 897, 936, 960, 1012, 1267, 1426, 1732, 1909 |
| BstV2I | GAAGAC | 2 cut(s) | 546, 1131 |
| BstX2I | RGATCY | 2 cut(s) | 689, 2026 |
| BstYI | RGATCY | 2 cut(s) | 689, 2026 |
| BstZ17I | GTATAC | 1 cut(s) | 750 |
| BstZI | CGGCCG | 1 cut(s) | 1968 |
| Bsu15I | ATCGAT | 3 cut(s) | 582, 813, 1140 |
| BsuI | GTATCC | 2 cut(s) | 526, 1967 |
| BsuTUI | ATCGAT | 3 cut(s) | 582, 813, 1140 |
| BtgI | CCRYGG | 2 cut(s) | 1857, 1875 |
| BtrI | CACGTC | 1 cut(s) | 1249 |
| BtsCI | GGATG | 9 cut(s) | 31, 513, 766, 847, 874, 984, 1090, 1302, 1987 |
| BtsI | GCAGTG | 1 cut(s) | 661 |
| BtsIMutI | CAGTG | 2 cut(s) | 661, 2062 |
| BveI | ACCTGC | 2 cut(s) | 1630, 1848 |
| Cac8I | GCNNGC | 4 cut(s) | 46, 567, 734, 2081 |
| CaiI | CAGNNNCTG | 1 cut(s) | 970 |
| CciI | TCATGA | 1 cut(s) | 1944 |
| CfoI | GCGC | 2 cut(s) | 910, 1759 |
| Cfr10I | RCCGGY | 1 cut(s) | 2072 |
| Cfr13I | GGNCC | 7 cut(s) | 487, 700, 1114, 1343, 1458, 1503, 2061 |
| Cfr42I | CCGCGG | 1 cut(s) | 1860 |
| Cfr9I | CCCGGG | 4 cut(s) | 702, 895, 1339, 1542 |
| ClaI | ATCGAT | 3 cut(s) | 582, 813, 1140 |
| CseI | GACGC | 2 cut(s) | 1136, 1241 |
| CspAI | ACCGGT | 1 cut(s) | 2072 |
| CviAII | CATG | 8 cut(s) | 712, 733, 1033, 1462, 1552, 1655, 1818, 1945 |
| DdeI | CTNAG | 7 cut(s) | 383, 592, 854, 863, 1701, 1716, 1752 |
| DraIII | CACNNNGTG | 1 cut(s) | 1252 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1153 |
| EaeI | YGGCCR | 3 cut(s) | 879, 1707, 1968 |
| EagI | CGGCCG | 1 cut(s) | 1968 |
| Eam1104I | CTCTTC | 6 cut(s) | 1040, 1065, 1313, 1571, 1758, 1893 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1153 |
| EarI | CTCTTC | 6 cut(s) | 1040, 1065, 1313, 1571, 1758, 1893 |
| Ecl136II | GAGCTC | 1 cut(s) | 1903 |
| EclXI | CGGCCG | 1 cut(s) | 1968 |
| Eco130I | CCWWGG | 3 cut(s) | 931, 1150, 1506 |
| Eco147I | AGGCCT | 1 cut(s) | 859 |
| Eco24I | GRGCYC | 1 cut(s) | 1905 |
| Eco31I | GGTCTC | 1 cut(s) | 2009 |
| Eco32I | GATATC | 1 cut(s) | 580 |
| Eco47I | GGWCC | 4 cut(s) | 487, 1343, 1458, 2061 |
| Eco52I | CGGCCG | 1 cut(s) | 1968 |
| Eco53kI | GAGCTC | 1 cut(s) | 1903 |
| Eco57I | CTGAAG | 4 cut(s) | 591, 845, 1496, 1631 |
| Eco88I | CYCGRG | 7 cut(s) | 702, 725, 782, 895, 1339, 1542, 1779 |
| Eco91I | GGTNACC | 1 cut(s) | 1469 |
| EcoICRI | GAGCTC | 1 cut(s) | 1903 |
| EcoO109I | RGGNCCY | 2 cut(s) | 487, 2061 |
| EcoO65I | GGTNACC | 1 cut(s) | 1469 |
| EcoRII | CCWGG | 4 cut(s) | 15, 521, 1116, 2007 |
| EcoRV | GATATC | 1 cut(s) | 580 |
| EcoT14I | CCWWGG | 3 cut(s) | 931, 1150, 1506 |
| EcoT38I | GRGCYC | 1 cut(s) | 1905 |
| ErhI | CCWWGG | 3 cut(s) | 931, 1150, 1506 |
| FaeI | CATG | 8 cut(s) | 715, 736, 1036, 1465, 1555, 1658, 1821, 1948 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1173, 1205 |
| FaqI | GGGAC | 6 cut(s) | 473, 778, 1356, 1700, 1796, 2047 |
| FatI | CATG | 8 cut(s) | 711, 732, 1032, 1461, 1551, 1654, 1817, 1944 |
| FauI | CCCGC | 4 cut(s) | 1677, 1752, 1864, 1992 |
| FblI | GTMKAC | 3 cut(s) | 632, 749, 1146 |
| FokI | GGATG | 9 cut(s) | 38, 520, 773, 854, 881, 991, 1097, 1309, 1994 |
| FriOI | GRGCYC | 1 cut(s) | 1905 |
| FspBI | CTAG | 2 cut(s) | 242, 1151 |
| GlaI | GCGC | 2 cut(s) | 909, 1758 |
| GsuI | CTGGAG | 4 cut(s) | 339, 1214, 1694, 2051 |
| HaeII | RGCGCY | 1 cut(s) | 911 |
| HgaI | GACGC | 2 cut(s) | 1136, 1241 |
| HhaI | GCGC | 2 cut(s) | 910, 1759 |
| Hin1I | GRCGYC | 1 cut(s) | 1128 |
| Hin1II | CATG | 8 cut(s) | 715, 736, 1036, 1465, 1555, 1658, 1821, 1948 |
| Hin6I | GCGC | 2 cut(s) | 908, 1757 |
| HinP1I | GCGC | 2 cut(s) | 908, 1757 |
| HincII | GTYRAC | 2 cut(s) | 633, 1147 |
| HindII | GTYRAC | 2 cut(s) | 633, 1147 |
| HphI | GGTGA | 9 cut(s) | 686, 984, 1192, 1229, 1328, 1481, 1654, 1754, 1780 |
| Hpy166II | GTNNAC | 5 cut(s) | 633, 750, 1147, 1220, 1576 |
| Hpy188III | TCNNGA | 7 cut(s) | 725, 1193, 1229, 1279, 1781, 1945, 2030 |
| Hpy8I | GTNNAC | 5 cut(s) | 633, 750, 1147, 1220, 1576 |
| Hpy99I | CGWCG | 9 cut(s) | 547, 634, 671, 779, 1235, 1253, 1274, 1357, 1715 |
| HpyCH4III | ACNGT | 3 cut(s) | 499, 769, 966 |
| HpyCH4IV | ACGT | 2 cut(s) | 1248, 1272 |
| HpyCH4V | TGCA | 3 cut(s) | 913, 1255, 1843 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 45, 566, 890, 979, 1411, 1462, 1706 |
| HpyF3I | CTNAG | 7 cut(s) | 383, 592, 854, 863, 1701, 1716, 1752 |
| HpySE526I | ACGT | 2 cut(s) | 1248, 1272 |
| Hsp92I | GRCGYC | 1 cut(s) | 1128 |
| Hsp92II | CATG | 8 cut(s) | 715, 736, 1036, 1465, 1555, 1658, 1821, 1948 |
| HspAI | GCGC | 2 cut(s) | 908, 1757 |
| KflI | GGGWCCC | 1 cut(s) | 2061 |
| KpnI | GGTACC | 8 cut(s) | 23, 89, 155, 221, 287, 428, 462, 498 |
| KspI | CCGCGG | 1 cut(s) | 1860 |
| LguI | GCTCTTC | 1 cut(s) | 1893 |
| LmnI | GCTCC | 6 cut(s) | 41, 850, 930, 1240, 1681, 1732 |
| Lsp1109I | GCAGC | 8 cut(s) | 897, 936, 960, 1012, 1267, 1426, 1732, 1909 |
| LweI | GCATC | 3 cut(s) | 832, 969, 1078 |
| MaeI | CTAG | 2 cut(s) | 242, 1151 |
| MaeII | ACGT | 2 cut(s) | 1248, 1272 |
| MaeIII | GTNAC | 3 cut(s) | 990, 1240, 1469 |
| MbiI | CCGCTC | 2 cut(s) | 1684, 2083 |
| MflI | RGATCY | 2 cut(s) | 689, 2026 |
| MhlI | GDGCHC | 2 cut(s) | 1564, 1905 |
| MluCI | AATT | 9 cut(s) | 71, 137, 203, 269, 344, 410, 608, 720, 1991 |
| MlyI | GAGTC | 7 cut(s) | 20, 86, 671, 1358, 1682, 1778, 1814 |
| MmeI | TCCRAC | 2 cut(s) | 690, 1293 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1188 |
| MslI | CAYNNNNRTG | 3 cut(s) | 1140, 1295, 1910 |
| MspA1I | CMGCKG | 5 cut(s) | 949, 1003, 1417, 1723, 1859 |
| Mva1269I | GAATGC | 1 cut(s) | 1018 |
| MvaI | CCWGG | 4 cut(s) | 17, 523, 1118, 2009 |
| MvnI | CGCG | 4 cut(s) | 884, 1730, 1759, 1859 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 45, 566, 890, 979, 1411, 1462, 1706 |
| NlaIII | CATG | 8 cut(s) | 715, 736, 1036, 1465, 1555, 1658, 1821, 1948 |
| NmeAIII | GCCGAG | 2 cut(s) | 623, 768 |
| NmuCI | GTSAC | 3 cut(s) | 990, 1240, 1469 |
| NspI | RCATGY | 1 cut(s) | 736 |
| PaeI | GCATGC | 1 cut(s) | 736 |
| PagI | TCATGA | 1 cut(s) | 1944 |
| PaqCI | CACCTGC | 2 cut(s) | 1630, 1848 |
| PceI | AGGCCT | 1 cut(s) | 859 |
| PciSI | GCTCTTC | 1 cut(s) | 1893 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 542, 629, 1437 |
| PctI | GAATGC | 1 cut(s) | 1018 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1188 |
| PfeI | GAWTC | 5 cut(s) | 512, 886, 919, 1528, 1547 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 1099, 1557 |
| PfoI | TCCNGGA | 1 cut(s) | 2007 |
| PinAI | ACCGGT | 1 cut(s) | 2072 |
| Ple19I | CGATCG | 1 cut(s) | 586 |
| PleI | GAGTC | 7 cut(s) | 19, 85, 670, 1357, 1682, 1778, 1813 |
| PpsI | GAGTC | 7 cut(s) | 19, 85, 670, 1357, 1682, 1778, 1813 |
| PpuMI | RGGWCCY | 2 cut(s) | 487, 2061 |
| PshAI | GACNNNNGTC | 2 cut(s) | 1247, 1348 |
| Psp124BI | GAGCTC | 1 cut(s) | 1905 |
| Psp5II | RGGWCCY | 2 cut(s) | 487, 2061 |
| Psp6I | CCWGG | 4 cut(s) | 15, 521, 1116, 2007 |
| PspEI | GGTNACC | 1 cut(s) | 1469 |
| PspGI | CCWGG | 4 cut(s) | 15, 521, 1116, 2007 |
| PspPI | GGNCC | 7 cut(s) | 487, 700, 1114, 1343, 1458, 1503, 2061 |
| PspPPI | RGGWCCY | 2 cut(s) | 487, 2061 |
| PstI | CTGCAG | 1 cut(s) | 1845 |
| PstNI | CAGNNNCTG | 1 cut(s) | 970 |
| PsuI | RGATCY | 2 cut(s) | 689, 2026 |
| PvuI | CGATCG | 1 cut(s) | 586 |
| PvuII | CAGCTG | 2 cut(s) | 1003, 1417 |
| RseI | CAYNNNNRTG | 3 cut(s) | 1140, 1295, 1910 |
| SacI | GAGCTC | 1 cut(s) | 1905 |
| SacII | CCGCGG | 1 cut(s) | 1860 |
| SalI | GTCGAC | 2 cut(s) | 631, 1145 |
| SapI | GCTCTTC | 1 cut(s) | 1893 |
| Sau96I | GGNCC | 7 cut(s) | 487, 700, 1114, 1343, 1458, 1503, 2061 |
| SbfI | CCTGCAGG | 1 cut(s) | 1845 |
| SchI | GAGTC | 7 cut(s) | 20, 86, 671, 1358, 1682, 1778, 1814 |
| SdaI | CCTGCAGG | 1 cut(s) | 1845 |
| SduI | GDGCHC | 2 cut(s) | 1564, 1905 |
| SfaNI | GCATC | 3 cut(s) | 832, 969, 1078 |
| SfcI | CTRYAG | 3 cut(s) | 962, 1800, 1841 |
| Sfr303I | CCGCGG | 1 cut(s) | 1860 |
| SgrBI | CCGCGG | 1 cut(s) | 1860 |
| SinI | GGWCC | 4 cut(s) | 487, 1343, 1458, 2061 |
| SmaI | CCCGGG | 4 cut(s) | 704, 897, 1341, 1544 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 1140, 1295, 1910 |
| SmlI | CTYRAG | 1 cut(s) | 1736 |
| SmoI | CTYRAG | 1 cut(s) | 1736 |
| SphI | GCATGC | 1 cut(s) | 736 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 1845 |
| Sse9I | AATT | 9 cut(s) | 71, 137, 203, 269, 344, 410, 608, 720, 1991 |
| SseBI | AGGCCT | 1 cut(s) | 859 |
| SspMI | CTAG | 2 cut(s) | 242, 1151 |
| SstI | GAGCTC | 1 cut(s) | 1905 |
| StuI | AGGCCT | 1 cut(s) | 859 |
| StyI | CCWWGG | 3 cut(s) | 931, 1150, 1506 |
| TaaI | ACNGT | 3 cut(s) | 499, 769, 966 |
| TaiI | ACGT | 2 cut(s) | 1251, 1275 |
| TaqI | TCGA | 8 cut(s) | 58, 582, 632, 813, 1140, 1146, 1157, 1491 |
| TaqII | GACCGA | 1 cut(s) | 1161 |
| TasI | AATT | 9 cut(s) | 71, 137, 203, 269, 344, 410, 608, 720, 1991 |
| TatI | WGTACW | 1 cut(s) | 1884 |
| TauI | GCSGC | 3 cut(s) | 884, 1970, 2039 |
| TfiI | GAWTC | 5 cut(s) | 512, 886, 919, 1528, 1547 |
| TscAI | CASTG | 2 cut(s) | 661, 2062 |
| TseFI | GTSAC | 3 cut(s) | 990, 1240, 1469 |
| TseI | GCWGC | 8 cut(s) | 910, 949, 973, 1000, 1255, 1414, 1720, 1922 |
| Tsp45I | GTSAC | 3 cut(s) | 990, 1240, 1469 |
| TspDTI | ATGAA | 6 cut(s) | 815, 1049, 1458, 1643, 1961, 1998 |
| TspMI | CCCGGG | 4 cut(s) | 702, 895, 1339, 1542 |
| TspRI | CASTG | 2 cut(s) | 661, 2062 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 1099, 1557 |
| VpaK11BI | GGWCC | 4 cut(s) | 487, 1343, 1458, 2061 |
| XapI | RAATTY | 7 cut(s) | 71, 137, 203, 269, 344, 410, 1991 |
| XceI | RCATGY | 1 cut(s) | 736 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2072 |
| XmaI | CCCGGG | 4 cut(s) | 702, 895, 1339, 1542 |
| XmaJI | CCTAGG | 1 cut(s) | 1150 |
| XmiI | GTMKAC | 3 cut(s) | 632, 749, 1146 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1188 |
| XspI | CTAG | 2 cut(s) | 242, 1151 |