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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1225 |
| AatII | GACGTC | 1 cut(s) | 606 |
| Acc36I | ACCTGC | 1 cut(s) | 1225 |
| Acc65I | GGTACC | 1 cut(s) | 1589 |
| AccB1I | GGYRCC | 3 cut(s) | 81, 902, 1589 |
| AccBSI | CCGCTC | 2 cut(s) | 1279, 1567 |
| AccI | GTMKAC | 1 cut(s) | 606 |
| AccII | CGCG | 3 cut(s) | 216, 218, 1397 |
| AclWI | GGATC | 5 cut(s) | 680, 834, 946, 1074, 1626 |
| AcoI | YGGCCR | 5 cut(s) | 3, 726, 944, 1302, 1506 |
| AcsI | RAATTY | 1 cut(s) | 1529 |
| AcuI | CTGAAG | 2 cut(s) | 1091, 1226 |
| AcyI | GRCGYC | 3 cut(s) | 603, 856, 1182 |
| AdeI | CACNNNGTG | 1 cut(s) | 712 |
| AflII | CTTAAG | 1 cut(s) | 238 |
| AgsI | TTSAA | 2 cut(s) | 641, 1291 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 613 |
| AjnI | CCWGG | 5 cut(s) | 480, 576, 897, 1116, 1545 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 824, 856 |
| AluBI | AGCT | 9 cut(s) | 49, 127, 353, 391, 487, 1012, 1267, 1330, 1441 |
| AluI | AGCT | 9 cut(s) | 49, 127, 353, 391, 487, 1012, 1267, 1330, 1441 |
| Alw21I | GWGCWC | 1 cut(s) | 489 |
| Alw26I | GTCTC | 4 cut(s) | 638, 1136, 1547, 1605 |
| AlwI | GGATC | 5 cut(s) | 680, 834, 946, 1074, 1626 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 94, 1015 |
| Ama87I | CYCGRG | 3 cut(s) | 19, 934, 1137 |
| ApeKI | GCWGC | 8 cut(s) | 34, 97, 124, 388, 1009, 1012, 1315, 1457 |
| ApoI | RAATTY | 1 cut(s) | 1529 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 1014, 1028, 1046, 1060 |
| Asp718I | GGTACC | 1 cut(s) | 1589 |
| AspA2I | CCTAGG | 1 cut(s) | 610 |
| AspLEI | GCGC | 3 cut(s) | 34, 218, 817 |
| AspS9I | GGNCC | 3 cut(s) | 175, 633, 703 |
| AsuHPI | GGTGA | 9 cut(s) | 108, 277, 689, 886, 923, 942, 1193, 1249, 1265 |
| AvaI | CYCGRG | 3 cut(s) | 19, 934, 1137 |
| AvaII | GGWCC | 2 cut(s) | 175, 633 |
| AvrII | CCTAGG | 1 cut(s) | 610 |
| BaeGI | GKGCMC | 1 cut(s) | 1159 |
| BalI | TGGCCA | 1 cut(s) | 5 |
| BanI | GGYRCC | 3 cut(s) | 81, 902, 1589 |
| BanII | GRGCYC | 1 cut(s) | 489 |
| BauI | CACGAG | 3 cut(s) | 38, 625, 1406 |
| BbsI | GAAGAC | 1 cut(s) | 753 |
| Bbv12I | GWGCWC | 1 cut(s) | 489 |
| BbvCI | CCTCAGC | 1 cut(s) | 1347 |
| BbvI | GCAGC | 8 cut(s) | 21, 84, 136, 400, 999, 1021, 1327, 1444 |
| BccI | CCATC | 9 cut(s) | 157, 439, 457, 535, 553, 1010, 1184, 1294, 1607 |
| BceAI | ACGGC | 5 cut(s) | 125, 931, 959, 962, 1113 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 839, 873 |
| BciT130I | CCWGG | 5 cut(s) | 482, 578, 899, 1118, 1547 |
| BciVI | GTATCC | 2 cut(s) | 665, 1189 |
| BcoDI | GTCTC | 4 cut(s) | 638, 1136, 1547, 1605 |
| BfaI | CTAG | 4 cut(s) | 296, 350, 611, 1517 |
| BfmI | CTRYAG | 1 cut(s) | 86 |
| BfoI | RGCGCY | 2 cut(s) | 35, 818 |
| BfrI | CTTAAG | 1 cut(s) | 238 |
| BfuAI | ACCTGC | 1 cut(s) | 1225 |
| BfuI | GTATCC | 2 cut(s) | 665, 1189 |
| BlnI | CCTAGG | 1 cut(s) | 610 |
| BlpI | GCTNAGC | 1 cut(s) | 1326 |
| Bme18I | GGWCC | 2 cut(s) | 175, 633 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 613 |
| BmeT110I | CYCGRG | 3 cut(s) | 19, 934, 1137 |
| BmgT120I | GGNCC | 3 cut(s) | 175, 633, 703 |
| BmiI | GGNNCC | 4 cut(s) | 83, 176, 904, 1591 |
| BmrI | ACTGGG | 2 cut(s) | 810, 819 |
| BmsI | GCATC | 4 cut(s) | 93, 577, 1047, 1140 |
| BmuI | ACTGGG | 2 cut(s) | 810, 819 |
| BpiI | GAAGAC | 1 cut(s) | 753 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 1268, 1300, 1430, 1462 |
| BpmI | CTGGAG | 3 cut(s) | 1289, 1400, 1568 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1347 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1326 |
| BpuEI | CTTGAG | 1 cut(s) | 1352 |
| Bsa29I | ATCGAT | 1 cut(s) | 1145 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1617 |
| BsaHI | GRCGYC | 3 cut(s) | 603, 856, 1182 |
| BsaI | GGTCTC | 3 cut(s) | 638, 1547, 1605 |
| BsaWI | WCCGGW | 1 cut(s) | 982 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 863, 893, 1412, 1442 |
| Bse118I | RCCGGY | 5 cut(s) | 752, 769, 852, 905, 1592 |
| Bse1I | ACTGG | 3 cut(s) | 805, 825, 889 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1617 |
| BseBI | CCWGG | 5 cut(s) | 482, 578, 899, 1118, 1547 |
| BseCI | ATCGAT | 1 cut(s) | 1145 |
| BseGI | GGATG | 6 cut(s) | 108, 450, 454, 546, 550, 898 |
| BseJI | GATNNNNATC | 1 cut(s) | 1617 |
| BseMII | CTCAG | 5 cut(s) | 1287, 1317, 1338, 1446, 1616 |
| BseNI | ACTGG | 3 cut(s) | 805, 825, 889 |
| BseRI | GAGGAG | 5 cut(s) | 778, 1205, 1373, 1376, 1433 |
| BseSI | GKGCMC | 1 cut(s) | 1159 |
| BseX3I | CGGCCG | 2 cut(s) | 944, 1506 |
| BseXI | GCAGC | 8 cut(s) | 21, 84, 136, 400, 999, 1021, 1327, 1444 |
| BseYI | CCCAGC | 2 cut(s) | 278, 888 |
| BsgI | GTGCAG | 2 cut(s) | 20, 578 |
| Bsh1236I | CGCG | 3 cut(s) | 216, 218, 1397 |
| Bsh1285I | CGRYCG | 4 cut(s) | 947, 1055, 1090, 1509 |
| BshNI | GGYRCC | 3 cut(s) | 81, 902, 1589 |
| BshVI | ATCGAT | 1 cut(s) | 1145 |
| BsiEI | CGRYCG | 4 cut(s) | 947, 1055, 1090, 1509 |
| BsiHKAI | GWGCWC | 1 cut(s) | 489 |
| BsiHKCI | CYCGRG | 3 cut(s) | 19, 934, 1137 |
| BslFI | GGGAC | 2 cut(s) | 188, 1295 |
| BsmAI | GTCTC | 4 cut(s) | 638, 1136, 1547, 1605 |
| BsmBI | CGTCTC | 1 cut(s) | 1136 |
| BsmFI | GGGAC | 2 cut(s) | 188, 1295 |
| BsmI | GAATGC | 1 cut(s) | 142 |
| Bso31I | GGTCTC | 3 cut(s) | 638, 1547, 1605 |
| BsoBI | CYCGRG | 3 cut(s) | 19, 934, 1137 |
| Bsp1286I | GDGCHC | 2 cut(s) | 489, 1159 |
| Bsp143I | GATC | 6 cut(s) | 685, 839, 928, 938, 1066, 1618 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1326 |
| Bsp19I | CCATGG | 1 cut(s) | 723 |
| BspCNI | CTCAG | 5 cut(s) | 1288, 1318, 1339, 1445, 1615 |
| BspDI | ATCGAT | 1 cut(s) | 1145 |
| BspFNI | CGCG | 3 cut(s) | 216, 218, 1397 |
| BspHI | TCATGA | 1 cut(s) | 1482 |
| BspLI | GGNNCC | 4 cut(s) | 83, 176, 904, 1591 |
| BspMI | ACCTGC | 1 cut(s) | 1225 |
| BspPI | GGATC | 5 cut(s) | 680, 834, 946, 1074, 1626 |
| BspT107I | GGYRCC | 3 cut(s) | 81, 902, 1589 |
| BspTI | CTTAAG | 1 cut(s) | 238 |
| BspTNI | GGTCTC | 3 cut(s) | 638, 1547, 1605 |
| BsrBI | CCGCTC | 2 cut(s) | 1279, 1567 |
| BsrFI | RCCGGY | 5 cut(s) | 752, 769, 852, 905, 1592 |
| BsrI | ACTGG | 3 cut(s) | 805, 825, 889 |
| BssAI | RCCGGY | 5 cut(s) | 752, 769, 852, 905, 1592 |
| BssMI | GATC | 6 cut(s) | 685, 839, 928, 938, 1066, 1618 |
| BssNI | GRCGYC | 3 cut(s) | 603, 856, 1182 |
| BssSI | CACGAG | 3 cut(s) | 38, 625, 1406 |
| BssT1I | CCWWGG | 4 cut(s) | 55, 610, 723, 958 |
| Bst2BI | CACGAG | 3 cut(s) | 38, 625, 1406 |
| Bst2UI | CCWGG | 5 cut(s) | 482, 578, 899, 1118, 1547 |
| Bst4CI | ACNGT | 4 cut(s) | 90, 265, 397, 677 |
| Bst6I | CTCTTC | 6 cut(s) | 404, 429, 500, 525, 636, 908 |
| BstACI | GRCGYC | 3 cut(s) | 603, 856, 1182 |
| BstAFI | CTTAAG | 1 cut(s) | 238 |
| BstC8I | GCNNGC | 5 cut(s) | 185, 1155, 1186, 1455, 1579 |
| BstDEI | CTNAG | 7 cut(s) | 993, 1296, 1311, 1326, 1347, 1432, 1602 |
| BstDSI | CCRYGG | 2 cut(s) | 723, 1395 |
| BstEII | GGTNACC | 1 cut(s) | 265 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1208 |
| BstF5I | GGATG | 6 cut(s) | 108, 450, 454, 546, 550, 898 |
| BstFNI | CGCG | 3 cut(s) | 216, 218, 1397 |
| BstH2I | RGCGCY | 2 cut(s) | 35, 818 |
| BstHHI | GCGC | 3 cut(s) | 34, 218, 817 |
| BstKTI | GATC | 6 cut(s) | 688, 842, 931, 941, 1069, 1621 |
| BstMAI | GTCTC | 4 cut(s) | 638, 1136, 1547, 1605 |
| BstMBI | GATC | 6 cut(s) | 685, 839, 928, 938, 1066, 1618 |
| BstMCI | CGRYCG | 4 cut(s) | 947, 1055, 1090, 1509 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 103, 193, 701, 1006, 1057, 1181, 1301 |
| BstNI | CCWGG | 5 cut(s) | 482, 578, 899, 1118, 1547 |
| BstNSI | RCATGY | 2 cut(s) | 472, 568 |
| BstPI | GGTNACC | 1 cut(s) | 265 |
| BstSFI | CTRYAG | 1 cut(s) | 86 |
| BstSLI | GKGCMC | 1 cut(s) | 1159 |
| BstUI | CGCG | 3 cut(s) | 216, 218, 1397 |
| BstV1I | GCAGC | 8 cut(s) | 21, 84, 136, 400, 999, 1021, 1327, 1444 |
| BstV2I | GAAGAC | 1 cut(s) | 753 |
| BstX2I | RGATCY | 2 cut(s) | 839, 1066 |
| BstYI | RGATCY | 2 cut(s) | 839, 1066 |
| BstZI | CGGCCG | 2 cut(s) | 944, 1506 |
| Bsu15I | ATCGAT | 1 cut(s) | 1145 |
| BsuI | GTATCC | 2 cut(s) | 665, 1189 |
| BsuTUI | ATCGAT | 1 cut(s) | 1145 |
| BtgI | CCRYGG | 2 cut(s) | 723, 1395 |
| BtgZI | GCGATG | 1 cut(s) | 822 |
| BtsCI | GGATG | 6 cut(s) | 108, 450, 454, 546, 550, 898 |
| BtsIMutI | CAGTG | 2 cut(s) | 832, 882 |
| BveI | ACCTGC | 1 cut(s) | 1225 |
| Cac8I | GCNNGC | 5 cut(s) | 185, 1155, 1186, 1455, 1579 |
| CaiI | CAGNNNCTG | 2 cut(s) | 94, 1015 |
| CciI | TCATGA | 1 cut(s) | 1482 |
| CfoI | GCGC | 3 cut(s) | 34, 218, 817 |
| Cfr10I | RCCGGY | 5 cut(s) | 752, 769, 852, 905, 1592 |
| Cfr13I | GGNCC | 3 cut(s) | 175, 633, 703 |
| Cfr42I | CCGCGG | 1 cut(s) | 1398 |
| Cfr9I | CCCGGG | 3 cut(s) | 19, 934, 1137 |
| ClaI | ATCGAT | 1 cut(s) | 1145 |
| CseI | GACGC | 5 cut(s) | 461, 557, 701, 845, 1190 |
| CviAII | CATG | 7 cut(s) | 469, 565, 724, 757, 1250, 1450, 1483 |
| DdeI | CTNAG | 7 cut(s) | 993, 1296, 1311, 1326, 1347, 1432, 1602 |
| DpnI | GATC | 6 cut(s) | 687, 841, 930, 940, 1068, 1620 |
| DpnII | GATC | 6 cut(s) | 685, 839, 928, 938, 1066, 1618 |
| DraIII | CACNNNGTG | 1 cut(s) | 712 |
| DriI | GACNNNNNGTC | 1 cut(s) | 613 |
| EaeI | YGGCCR | 5 cut(s) | 3, 726, 944, 1302, 1506 |
| EagI | CGGCCG | 2 cut(s) | 944, 1506 |
| Eam1104I | CTCTTC | 6 cut(s) | 404, 429, 500, 525, 636, 908 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 613 |
| EarI | CTCTTC | 6 cut(s) | 404, 429, 500, 525, 636, 908 |
| Ecl136II | GAGCTC | 1 cut(s) | 487 |
| EclXI | CGGCCG | 2 cut(s) | 944, 1506 |
| Eco130I | CCWWGG | 4 cut(s) | 55, 610, 723, 958 |
| Eco24I | GRGCYC | 1 cut(s) | 489 |
| Eco31I | GGTCTC | 3 cut(s) | 638, 1547, 1605 |
| Eco32I | GATATC | 1 cut(s) | 1078 |
| Eco47I | GGWCC | 2 cut(s) | 175, 633 |
| Eco52I | CGGCCG | 2 cut(s) | 944, 1506 |
| Eco53kI | GAGCTC | 1 cut(s) | 487 |
| Eco57I | CTGAAG | 2 cut(s) | 1091, 1226 |
| Eco88I | CYCGRG | 3 cut(s) | 19, 934, 1137 |
| Eco91I | GGTNACC | 1 cut(s) | 265 |
| EcoICRI | GAGCTC | 1 cut(s) | 487 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1208 |
| EcoO109I | RGGNCCY | 1 cut(s) | 175 |
| EcoO65I | GGTNACC | 1 cut(s) | 265 |
| EcoRII | CCWGG | 5 cut(s) | 480, 576, 897, 1116, 1545 |
| EcoRV | GATATC | 1 cut(s) | 1078 |
| EcoT14I | CCWWGG | 4 cut(s) | 55, 610, 723, 958 |
| EcoT38I | GRGCYC | 1 cut(s) | 489 |
| ErhI | CCWWGG | 4 cut(s) | 55, 610, 723, 958 |
| Esp3I | CGTCTC | 1 cut(s) | 1136 |
| FaeI | CATG | 7 cut(s) | 472, 568, 727, 760, 1253, 1453, 1486 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 633, 665 |
| FaqI | GGGAC | 2 cut(s) | 188, 1295 |
| FatI | CATG | 7 cut(s) | 468, 564, 723, 756, 1249, 1449, 1482 |
| FauI | CCCGC | 9 cut(s) | 250, 982, 1146, 1272, 1402, 1530, 1560, 1570, 1589 |
| FblI | GTMKAC | 1 cut(s) | 606 |
| FokI | GGATG | 6 cut(s) | 115, 457, 461, 553, 557, 905 |
| FriOI | GRGCYC | 1 cut(s) | 489 |
| FspBI | CTAG | 4 cut(s) | 296, 350, 611, 1517 |
| GlaI | GCGC | 3 cut(s) | 33, 217, 816 |
| GsaI | CCCAGC | 2 cut(s) | 282, 892 |
| GsuI | CTGGAG | 3 cut(s) | 1289, 1400, 1568 |
| HaeII | RGCGCY | 2 cut(s) | 35, 818 |
| HgaI | GACGC | 5 cut(s) | 461, 557, 701, 845, 1190 |
| HhaI | GCGC | 3 cut(s) | 34, 218, 817 |
| Hin1I | GRCGYC | 3 cut(s) | 603, 856, 1182 |
| Hin1II | CATG | 7 cut(s) | 472, 568, 727, 760, 1253, 1453, 1486 |
| Hin6I | GCGC | 3 cut(s) | 32, 216, 815 |
| HinP1I | GCGC | 3 cut(s) | 32, 216, 815 |
| HincII | GTYRAC | 1 cut(s) | 607 |
| HindII | GTYRAC | 1 cut(s) | 607 |
| HinfI | GANTC | 7 cut(s) | 10, 43, 628, 1123, 1142, 1283, 1373 |
| HphI | GGTGA | 9 cut(s) | 108, 277, 689, 886, 923, 942, 1193, 1249, 1265 |
| Hpy166II | GTNNAC | 5 cut(s) | 223, 607, 680, 882, 1171 |
| Hpy188I | TCNGA | 3 cut(s) | 1071, 1435, 1605 |
| Hpy188III | TCNNGA | 4 cut(s) | 689, 1379, 1483, 1517 |
| Hpy8I | GTNNAC | 5 cut(s) | 223, 607, 680, 882, 1171 |
| Hpy99I | CGWCG | 7 cut(s) | 605, 608, 695, 734, 861, 1040, 1184 |
| HpyAV | CCTTC | 8 cut(s) | 207, 963, 1066, 1201, 1252, 1256, 1267, 1285 |
| HpyCH4III | ACNGT | 4 cut(s) | 90, 265, 397, 677 |
| HpyCH4IV | ACGT | 4 cut(s) | 603, 732, 878, 1215 |
| HpyCH4V | TGCA | 3 cut(s) | 37, 595, 715 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 103, 193, 701, 1006, 1057, 1181, 1301 |
| HpyF3I | CTNAG | 7 cut(s) | 993, 1296, 1311, 1326, 1347, 1432, 1602 |
| HpySE526I | ACGT | 4 cut(s) | 603, 732, 878, 1215 |
| Hsp92I | GRCGYC | 3 cut(s) | 603, 856, 1182 |
| Hsp92II | CATG | 7 cut(s) | 472, 568, 727, 760, 1253, 1453, 1486 |
| HspAI | GCGC | 3 cut(s) | 32, 216, 815 |
| KpnI | GGTACC | 1 cut(s) | 1593 |
| KspI | CCGCGG | 1 cut(s) | 1398 |
| Kzo9I | GATC | 6 cut(s) | 685, 839, 928, 938, 1066, 1618 |
| LmnI | GCTCC | 6 cut(s) | 54, 484, 492, 700, 804, 1276 |
| Lsp1109I | GCAGC | 8 cut(s) | 21, 84, 136, 400, 999, 1021, 1327, 1444 |
| LweI | GCATC | 4 cut(s) | 93, 577, 1047, 1140 |
| MaeI | CTAG | 4 cut(s) | 296, 350, 611, 1517 |
| MaeII | ACGT | 4 cut(s) | 603, 732, 878, 1215 |
| MaeIII | GTNAC | 5 cut(s) | 114, 265, 298, 874, 948 |
| MalI | GATC | 6 cut(s) | 687, 841, 930, 940, 1068, 1620 |
| MbiI | CCGCTC | 2 cut(s) | 1279, 1567 |
| MboI | GATC | 6 cut(s) | 685, 839, 928, 938, 1066, 1618 |
| MboII | GAAGA | 8 cut(s) | 421, 446, 517, 542, 596, 653, 758, 925 |
| MflI | RGATCY | 2 cut(s) | 839, 1066 |
| MhlI | GDGCHC | 2 cut(s) | 489, 1159 |
| MlsI | TGGCCA | 1 cut(s) | 5 |
| MluCI | AATT | 2 cut(s) | 735, 1529 |
| MluNI | TGGCCA | 1 cut(s) | 5 |
| MlyI | GAGTC | 3 cut(s) | 637, 1277, 1367 |
| Mox20I | TGGCCA | 1 cut(s) | 5 |
| MscI | TGGCCA | 1 cut(s) | 5 |
| MseI | TTAA | 3 cut(s) | 239, 1164, 1491 |
| MslI | CAYNNNNRTG | 3 cut(s) | 473, 569, 755 |
| Msp20I | TGGCCA | 1 cut(s) | 5 |
| MspA1I | CMGCKG | 6 cut(s) | 127, 278, 391, 1012, 1318, 1397 |
| MspCI | CTTAAG | 1 cut(s) | 238 |
| Mva1269I | GAATGC | 1 cut(s) | 142 |
| MvaI | CCWGG | 5 cut(s) | 482, 578, 899, 1118, 1547 |
| MvnI | CGCG | 3 cut(s) | 216, 218, 1397 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 103, 193, 701, 1006, 1057, 1181, 1301 |
| NcoI | CCATGG | 1 cut(s) | 723 |
| NdeII | GATC | 6 cut(s) | 685, 839, 928, 938, 1066, 1618 |
| NlaIII | CATG | 7 cut(s) | 472, 568, 727, 760, 1253, 1453, 1486 |
| NlaIV | GGNNCC | 4 cut(s) | 83, 176, 904, 1591 |
| NmuCI | GTSAC | 4 cut(s) | 114, 265, 874, 948 |
| NspI | RCATGY | 2 cut(s) | 472, 568 |
| PagI | TCATGA | 1 cut(s) | 1482 |
| PaqCI | CACCTGC | 1 cut(s) | 1225 |
| PcsI | WCGNNNNNNNCGW | 4 cut(s) | 623, 1032, 1044, 1185 |
| PctI | GAATGC | 1 cut(s) | 142 |
| PfeI | GAWTC | 4 cut(s) | 10, 43, 1123, 1142 |
| PfoI | TCCNGGA | 1 cut(s) | 1545 |
| PleI | GAGTC | 3 cut(s) | 636, 1277, 1367 |
| PpsI | GAGTC | 3 cut(s) | 636, 1277, 1367 |
| PpuMI | RGGWCCY | 1 cut(s) | 175 |
| Psp124BI | GAGCTC | 1 cut(s) | 489 |
| Psp5II | RGGWCCY | 1 cut(s) | 175 |
| Psp6I | CCWGG | 5 cut(s) | 480, 576, 897, 1116, 1545 |
| PspEI | GGTNACC | 1 cut(s) | 265 |
| PspFI | CCCAGC | 2 cut(s) | 278, 888 |
| PspGI | CCWGG | 5 cut(s) | 480, 576, 897, 1116, 1545 |
| PspN4I | GGNNCC | 4 cut(s) | 83, 176, 904, 1591 |
| PspPI | GGNCC | 3 cut(s) | 175, 633, 703 |
| PspPPI | RGGWCCY | 1 cut(s) | 175 |
| PstNI | CAGNNNCTG | 2 cut(s) | 94, 1015 |
| PsuI | RGATCY | 2 cut(s) | 839, 1066 |
| PvuII | CAGCTG | 3 cut(s) | 127, 391, 1012 |
| RseI | CAYNNNNRTG | 3 cut(s) | 473, 569, 755 |
| SacI | GAGCTC | 1 cut(s) | 489 |
| SacII | CCGCGG | 1 cut(s) | 1398 |
| SalI | GTCGAC | 1 cut(s) | 605 |
| SaqAI | TTAA | 3 cut(s) | 239, 1164, 1491 |
| Sau3AI | GATC | 6 cut(s) | 685, 839, 928, 938, 1066, 1618 |
| Sau96I | GGNCC | 3 cut(s) | 175, 633, 703 |
| SchI | GAGTC | 3 cut(s) | 637, 1277, 1367 |
| SduI | GDGCHC | 2 cut(s) | 489, 1159 |
| SfaNI | GCATC | 4 cut(s) | 93, 577, 1047, 1140 |
| SfcI | CTRYAG | 1 cut(s) | 86 |
| Sfr303I | CCGCGG | 1 cut(s) | 1398 |
| SgrAI | CRCCGGYG | 1 cut(s) | 852 |
| SgrBI | CCGCGG | 1 cut(s) | 1398 |
| SinI | GGWCC | 2 cut(s) | 175, 633 |
| SmaI | CCCGGG | 3 cut(s) | 21, 936, 1139 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 473, 569, 755 |
| SmlI | CTYRAG | 2 cut(s) | 238, 1331 |
| SmoI | CTYRAG | 2 cut(s) | 238, 1331 |
| Sse9I | AATT | 2 cut(s) | 735, 1529 |
| SspMI | CTAG | 4 cut(s) | 296, 350, 611, 1517 |
| SstI | GAGCTC | 1 cut(s) | 489 |
| StyI | CCWWGG | 4 cut(s) | 55, 610, 723, 958 |
| TaaI | ACNGT | 4 cut(s) | 90, 265, 397, 677 |
| TaiI | ACGT | 4 cut(s) | 606, 735, 881, 1218 |
| TaqII | GACCGA | 1 cut(s) | 621 |
| TasI | AATT | 2 cut(s) | 735, 1529 |
| TatI | WGTACW | 2 cut(s) | 234, 1422 |
| TauI | GCSGC | 6 cut(s) | 196, 278, 295, 1307, 1508, 1577 |
| TfiI | GAWTC | 4 cut(s) | 10, 43, 1123, 1142 |
| Tru1I | TTAA | 3 cut(s) | 239, 1164, 1491 |
| Tru9I | TTAA | 3 cut(s) | 239, 1164, 1491 |
| TscAI | CASTG | 2 cut(s) | 832, 889 |
| TseFI | GTSAC | 4 cut(s) | 114, 265, 874, 948 |
| TseI | GCWGC | 8 cut(s) | 34, 97, 124, 388, 1009, 1012, 1315, 1457 |
| Tsp45I | GTSAC | 4 cut(s) | 114, 265, 874, 948 |
| TspDTI | ATGAA | 2 cut(s) | 1238, 1499 |
| TspMI | CCCGGG | 3 cut(s) | 19, 934, 1137 |
| TspRI | CASTG | 2 cut(s) | 832, 889 |
| Vha464I | CTTAAG | 1 cut(s) | 238 |
| VpaK11BI | GGWCC | 2 cut(s) | 175, 633 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1208 |
| XapI | RAATTY | 1 cut(s) | 1529 |
| XbaI | TCTAGA | 1 cut(s) | 1516 |
| XceI | RCATGY | 2 cut(s) | 472, 568 |
| XmaI | CCCGGG | 3 cut(s) | 19, 934, 1137 |
| XmaJI | CCTAGG | 1 cut(s) | 610 |
| XmiI | GTMKAC | 1 cut(s) | 606 |
| XspI | CTAG | 4 cut(s) | 296, 350, 611, 1517 |
| ZraI | GACGTC | 1 cut(s) | 604 |