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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 429 |
| AccII | CGCG | 1 cut(s) | 269 |
| AccIII | TCCGGA | 1 cut(s) | 451 |
| AciI | CCGC | 4 cut(s) | 202, 221, 279, 336 |
| AcsI | RAATTY | 1 cut(s) | 10 |
| AcyI | GRCGYC | 1 cut(s) | 276 |
| AfaI | GTAC | 3 cut(s) | 131, 266, 497 |
| AfeI | AGCGCT | 1 cut(s) | 316 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 196, 244, 304, 429, 469 |
| AgsI | TTSAA | 2 cut(s) | 259, 565 |
| AluBI | AGCT | 2 cut(s) | 358, 514 |
| AluI | AGCT | 2 cut(s) | 358, 514 |
| Alw21I | GWGCWC | 1 cut(s) | 392 |
| Alw26I | GTCTC | 1 cut(s) | 81 |
| Ama87I | CYCGRG | 2 cut(s) | 243, 391 |
| Aor13HI | TCCGGA | 1 cut(s) | 451 |
| Aor51HI | AGCGCT | 1 cut(s) | 316 |
| AoxI | GGCC | 2 cut(s) | 483, 546 |
| ApeKI | GCWGC | 2 cut(s) | 533, 578 |
| ApoI | RAATTY | 1 cut(s) | 10 |
| AspLEI | GCGC | 2 cut(s) | 317, 462 |
| AspS9I | GGNCC | 1 cut(s) | 159 |
| AsuHPI | GGTGA | 1 cut(s) | 392 |
| AvaI | CYCGRG | 2 cut(s) | 243, 391 |
| AvaII | GGWCC | 1 cut(s) | 159 |
| BanII | GRGCYC | 1 cut(s) | 506 |
| Bbv12I | GWGCWC | 1 cut(s) | 392 |
| BbvI | GCAGC | 1 cut(s) | 520 |
| BceAI | ACGGC | 2 cut(s) | 470, 533 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 212, 246, 389, 423 |
| BcoDI | GTCTC | 1 cut(s) | 81 |
| BfaI | CTAG | 1 cut(s) | 589 |
| BfoI | RGCGCY | 2 cut(s) | 318, 463 |
| BisI | GCNGC | 4 cut(s) | 279, 337, 534, 579 |
| BlsI | GCNGC | 4 cut(s) | 280, 338, 535, 580 |
| Bme18I | GGWCC | 1 cut(s) | 159 |
| BmeT110I | CYCGRG | 2 cut(s) | 243, 391 |
| BmgT120I | GGNCC | 1 cut(s) | 159 |
| BmsI | GCATC | 2 cut(s) | 323, 397 |
| BoxI | GACNNNNGTC | 1 cut(s) | 49 |
| BpuEI | CTTGAG | 1 cut(s) | 522 |
| BsaHI | GRCGYC | 1 cut(s) | 276 |
| BsaJI | CCNNGG | 2 cut(s) | 282, 486 |
| BsaWI | WCCGGW | 2 cut(s) | 329, 451 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 196, 244, 304, 429, 469 |
| Bse118I | RCCGGY | 1 cut(s) | 581 |
| BseAI | TCCGGA | 1 cut(s) | 451 |
| BseDI | CCNNGG | 2 cut(s) | 282, 486 |
| BseGI | GGATG | 3 cut(s) | 38, 338, 448 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 196, 244, 304, 429, 469 |
| BseRI | GAGGAG | 3 cut(s) | 69, 470, 506 |
| BseXI | GCAGC | 1 cut(s) | 520 |
| Bsh1236I | CGCG | 1 cut(s) | 269 |
| BshFI | GGCC | 2 cut(s) | 485, 548 |
| BsiHKAI | GWGCWC | 1 cut(s) | 392 |
| BsiHKCI | CYCGRG | 2 cut(s) | 243, 391 |
| BsiSI | CCGG | 3 cut(s) | 330, 452, 582 |
| BslFI | GGGAC | 1 cut(s) | 408 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 196, 244, 304, 429, 469 |
| BsmAI | GTCTC | 1 cut(s) | 81 |
| BsmFI | GGGAC | 1 cut(s) | 408 |
| BsnI | GGCC | 2 cut(s) | 485, 548 |
| BsoBI | CYCGRG | 2 cut(s) | 243, 391 |
| Bsp1286I | GDGCHC | 2 cut(s) | 392, 506 |
| Bsp13I | TCCGGA | 1 cut(s) | 451 |
| Bsp143I | GATC | 1 cut(s) | 445 |
| BspACI | CCGC | 4 cut(s) | 202, 221, 279, 336 |
| BspANI | GGCC | 2 cut(s) | 485, 548 |
| BspEI | TCCGGA | 1 cut(s) | 451 |
| BspFNI | CGCG | 1 cut(s) | 269 |
| BsrFI | RCCGGY | 1 cut(s) | 581 |
| BssAI | RCCGGY | 1 cut(s) | 581 |
| BssECI | CCNNGG | 2 cut(s) | 282, 486 |
| BssMI | GATC | 1 cut(s) | 445 |
| BssNI | GRCGYC | 1 cut(s) | 276 |
| Bst4CI | ACNGT | 1 cut(s) | 91 |
| Bst6I | CTCTTC | 3 cut(s) | 16, 260, 378 |
| BstACI | GRCGYC | 1 cut(s) | 276 |
| BstC8I | GCNNGC | 2 cut(s) | 168, 204 |
| BstDSI | CCRYGG | 1 cut(s) | 486 |
| BstF5I | GGATG | 3 cut(s) | 38, 338, 448 |
| BstFNI | CGCG | 1 cut(s) | 269 |
| BstH2I | RGCGCY | 2 cut(s) | 318, 463 |
| BstHHI | GCGC | 2 cut(s) | 317, 462 |
| BstKTI | GATC | 1 cut(s) | 448 |
| BstMAI | GTCTC | 1 cut(s) | 81 |
| BstMBI | GATC | 1 cut(s) | 445 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 248, 275, 323, 364 |
| BstNSI | RCATGY | 1 cut(s) | 73 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 49 |
| BstUI | CGCG | 1 cut(s) | 269 |
| BstV1I | GCAGC | 1 cut(s) | 520 |
| BsuRI | GGCC | 2 cut(s) | 485, 548 |
| BtgI | CCRYGG | 1 cut(s) | 486 |
| BtsCI | GGATG | 3 cut(s) | 38, 338, 448 |
| Cac8I | GCNNGC | 2 cut(s) | 168, 204 |
| CfoI | GCGC | 2 cut(s) | 317, 462 |
| Cfr10I | RCCGGY | 1 cut(s) | 581 |
| Cfr13I | GGNCC | 1 cut(s) | 159 |
| CseI | GACGC | 1 cut(s) | 284 |
| Csp6I | GTAC | 3 cut(s) | 130, 265, 496 |
| CviAII | CATG | 3 cut(s) | 70, 163, 429 |
| CviJI | RGCY | 9 cut(s) | 106, 206, 358, 421, 485, 504, 514, 548, 581 |
| CviKI_1 | RGCY | 9 cut(s) | 106, 206, 358, 421, 485, 504, 514, 548, 581 |
| CviQI | GTAC | 3 cut(s) | 130, 265, 496 |
| DpnI | GATC | 1 cut(s) | 447 |
| DpnII | GATC | 1 cut(s) | 445 |
| Eam1104I | CTCTTC | 3 cut(s) | 16, 260, 378 |
| EarI | CTCTTC | 3 cut(s) | 16, 260, 378 |
| Eco24I | GRGCYC | 1 cut(s) | 506 |
| Eco47I | GGWCC | 1 cut(s) | 159 |
| Eco47III | AGCGCT | 1 cut(s) | 316 |
| Eco88I | CYCGRG | 2 cut(s) | 243, 391 |
| EcoRI | GAATTC | 1 cut(s) | 10 |
| EcoT38I | GRGCYC | 1 cut(s) | 506 |
| FaeI | CATG | 3 cut(s) | 73, 166, 432 |
| FaiI | YATR | 7 cut(s) | 71, 87, 164, 185, 430, 524, 557 |
| FaqI | GGGAC | 1 cut(s) | 408 |
| FatI | CATG | 3 cut(s) | 69, 162, 428 |
| FauI | CCCGC | 2 cut(s) | 195, 228 |
| Fnu4HI | GCNGC | 4 cut(s) | 279, 337, 534, 579 |
| FokI | GGATG | 3 cut(s) | 25, 345, 435 |
| FriOI | GRGCYC | 1 cut(s) | 506 |
| Fsp4HI | GCNGC | 4 cut(s) | 279, 337, 534, 579 |
| FspBI | CTAG | 1 cut(s) | 589 |
| GlaI | GCGC | 2 cut(s) | 316, 461 |
| GluI | GCNGC | 4 cut(s) | 279, 337, 534, 579 |
| HaeII | RGCGCY | 2 cut(s) | 318, 463 |
| HaeIII | GGCC | 2 cut(s) | 485, 548 |
| HapII | CCGG | 3 cut(s) | 330, 452, 582 |
| HgaI | GACGC | 1 cut(s) | 284 |
| HhaI | GCGC | 2 cut(s) | 317, 462 |
| Hin1I | GRCGYC | 1 cut(s) | 276 |
| Hin1II | CATG | 3 cut(s) | 73, 166, 432 |
| Hin6I | GCGC | 2 cut(s) | 315, 460 |
| HinP1I | GCGC | 2 cut(s) | 315, 460 |
| HinfI | GANTC | 4 cut(s) | 50, 229, 396, 415 |
| HpaII | CCGG | 3 cut(s) | 330, 452, 582 |
| HphI | GGTGA | 1 cut(s) | 392 |
| Hpy166II | GTNNAC | 1 cut(s) | 265 |
| Hpy188I | TCNGA | 1 cut(s) | 323 |
| Hpy188III | TCNNGA | 5 cut(s) | 42, 245, 393, 452, 539 |
| Hpy8I | GTNNAC | 1 cut(s) | 265 |
| Hpy99I | CGWCG | 2 cut(s) | 58, 237 |
| HpyAV | CCTTC | 3 cut(s) | 172, 427, 473 |
| HpyCH4III | ACNGT | 1 cut(s) | 91 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 248, 275, 323, 364 |
| Hsp92I | GRCGYC | 1 cut(s) | 276 |
| Hsp92II | CATG | 3 cut(s) | 73, 166, 432 |
| HspAI | GCGC | 2 cut(s) | 315, 460 |
| Kpn2I | TCCGGA | 1 cut(s) | 451 |
| Kzo9I | GATC | 1 cut(s) | 445 |
| LmnI | GCTCC | 2 cut(s) | 355, 457 |
| LpnPI | CCDG | 4 cut(s) | 55, 131, 343, 465 |
| Lsp1109I | GCAGC | 1 cut(s) | 520 |
| LweI | GCATC | 2 cut(s) | 323, 397 |
| MaeI | CTAG | 1 cut(s) | 589 |
| MalI | GATC | 1 cut(s) | 447 |
| MboI | GATC | 1 cut(s) | 445 |
| MboII | GAAGA | 5 cut(s) | 33, 41, 247, 299, 395 |
| MhlI | GDGCHC | 2 cut(s) | 392, 506 |
| MluCI | AATT | 2 cut(s) | 10, 528 |
| MlyI | GAGTC | 2 cut(s) | 59, 390 |
| MroI | TCCGGA | 1 cut(s) | 451 |
| MseI | TTAA | 2 cut(s) | 111, 527 |
| MslI | CAYNNNNRTG | 1 cut(s) | 521 |
| MspI | CCGG | 3 cut(s) | 330, 452, 582 |
| MvnI | CGCG | 1 cut(s) | 269 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 248, 275, 323, 364 |
| NdeII | GATC | 1 cut(s) | 445 |
| NlaIII | CATG | 3 cut(s) | 73, 166, 432 |
| NmeAIII | GCCGAG | 1 cut(s) | 307 |
| NspI | RCATGY | 1 cut(s) | 73 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 138 |
| PfeI | GAWTC | 2 cut(s) | 229, 415 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 429 |
| PkrI | GCNGC | 4 cut(s) | 280, 338, 535, 580 |
| PleI | GAGTC | 2 cut(s) | 58, 390 |
| PpsI | GAGTC | 2 cut(s) | 58, 390 |
| PshAI | GACNNNNGTC | 1 cut(s) | 49 |
| PspPI | GGNCC | 1 cut(s) | 159 |
| RsaI | GTAC | 3 cut(s) | 131, 266, 497 |
| RsaNI | GTAC | 3 cut(s) | 130, 265, 496 |
| RseI | CAYNNNNRTG | 1 cut(s) | 521 |
| SaqAI | TTAA | 2 cut(s) | 111, 527 |
| SatI | GCNGC | 4 cut(s) | 279, 337, 534, 579 |
| Sau3AI | GATC | 1 cut(s) | 445 |
| Sau96I | GGNCC | 1 cut(s) | 159 |
| SchI | GAGTC | 2 cut(s) | 59, 390 |
| SduI | GDGCHC | 2 cut(s) | 392, 506 |
| SetI | ASST | 5 cut(s) | 120, 192, 360, 382, 516 |
| SfaNI | GCATC | 2 cut(s) | 323, 397 |
| SinI | GGWCC | 1 cut(s) | 159 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 521 |
| SmlI | CTYRAG | 1 cut(s) | 537 |
| SmoI | CTYRAG | 1 cut(s) | 537 |
| Sse9I | AATT | 2 cut(s) | 10, 528 |
| SsiI | CCGC | 4 cut(s) | 202, 221, 279, 336 |
| SspMI | CTAG | 1 cut(s) | 589 |
| TaaI | ACNGT | 1 cut(s) | 91 |
| TaqI | TCGA | 4 cut(s) | 192, 361, 376, 399 |
| TasI | AATT | 2 cut(s) | 10, 528 |
| TatI | WGTACW | 1 cut(s) | 495 |
| TauI | GCSGC | 2 cut(s) | 281, 339 |
| TfiI | GAWTC | 2 cut(s) | 229, 415 |
| Tru1I | TTAA | 2 cut(s) | 111, 527 |
| Tru9I | TTAA | 2 cut(s) | 111, 527 |
| TseI | GCWGC | 2 cut(s) | 533, 578 |
| TspDTI | ATGAA | 2 cut(s) | 159, 572 |
| TspGWI | ACGGA | 1 cut(s) | 368 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 429 |
| VpaK11BI | GGWCC | 1 cut(s) | 159 |
| XapI | RAATTY | 1 cut(s) | 10 |
| XceI | RCATGY | 1 cut(s) | 73 |
| XspI | CTAG | 1 cut(s) | 589 |