No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1747 |
| AatII | GACGTC | 1 cut(s) | 1218 |
| Acc36I | ACCTGC | 1 cut(s) | 1747 |
| Acc65I | GGTACC | 5 cut(s) | 167, 261, 947, 1514, 2091 |
| AccB1I | GGYRCC | 5 cut(s) | 167, 261, 947, 1514, 2091 |
| AccBSI | CCGCTC | 1 cut(s) | 1801 |
| AccI | GTMKAC | 4 cut(s) | 276, 486, 634, 1218 |
| AccII | CGCG | 6 cut(s) | 46, 765, 767, 1320, 1325, 1976 |
| AccIII | TCCGGA | 1 cut(s) | 1594 |
| AclWI | GGATC | 6 cut(s) | 58, 220, 370, 1292, 1446, 1971 |
| AcoI | YGGCCR | 9 cut(s) | 390, 552, 714, 1338, 1498, 1824, 2085, 2157, 2214 |
| AcsI | RAATTY | 2 cut(s) | 134, 2108 |
| AcuI | CTGAAG | 2 cut(s) | 518, 1748 |
| AcvI | CACGTG | 1 cut(s) | 56 |
| AcyI | GRCGYC | 3 cut(s) | 1215, 1305, 1704 |
| AflIII | ACRYGT | 3 cut(s) | 44, 53, 55 |
| AgeI | ACCGGT | 3 cut(s) | 258, 944, 1517 |
| AgsI | TTSAA | 4 cut(s) | 134, 245, 1253, 1687 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1225 |
| AleI | CACNNNNGTG | 1 cut(s) | 1367 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 284, 316 |
| Alw21I | GWGCWC | 1 cut(s) | 1049 |
| Alw26I | GTCTC | 4 cut(s) | 1250, 1936, 2126, 2241 |
| AlwI | GGATC | 6 cut(s) | 58, 220, 370, 1292, 1446, 1971 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 643 |
| Ama87I | CYCGRG | 6 cut(s) | 398, 568, 1011, 1546, 1659, 1896 |
| Aor13HI | TCCGGA | 1 cut(s) | 1594 |
| ApeKI | GCWGC | 9 cut(s) | 583, 646, 673, 937, 1327, 1837, 1916, 2036, 2039 |
| ApoI | RAATTY | 2 cut(s) | 134, 2108 |
| AseI | ATTAAT | 1 cut(s) | 1169 |
| AsiGI | ACCGGT | 3 cut(s) | 258, 944, 1517 |
| Asp718I | GGTACC | 5 cut(s) | 167, 261, 947, 1514, 2091 |
| AspLEI | GCGC | 4 cut(s) | 583, 767, 1322, 1327 |
| AspS9I | GGNCC | 9 cut(s) | 160, 300, 373, 438, 724, 839, 967, 1550, 1556 |
| AsuHPI | GGTGA | 9 cut(s) | 359, 657, 826, 1301, 1451, 1498, 1535, 1771, 1871 |
| AvaI | CYCGRG | 6 cut(s) | 398, 568, 1011, 1546, 1659, 1896 |
| AvaII | GGWCC | 6 cut(s) | 160, 300, 438, 724, 967, 1550 |
| BaeGI | GKGCMC | 2 cut(s) | 1395, 1681 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 159, 192 |
| BalI | TGGCCA | 3 cut(s) | 392, 716, 1826 |
| BanI | GGYRCC | 5 cut(s) | 167, 261, 947, 1514, 2091 |
| BanII | GRGCYC | 1 cut(s) | 1049 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 404, 436 |
| BauI | CACGAG | 3 cut(s) | 587, 1237, 1985 |
| BbrPI | CACGTG | 1 cut(s) | 56 |
| Bbv12I | GWGCWC | 1 cut(s) | 1049 |
| BbvI | GCAGC | 9 cut(s) | 570, 633, 685, 949, 1339, 1849, 1903, 2023, 2026 |
| BccI | CCATC | 7 cut(s) | 175, 536, 706, 1147, 1706, 1816, 2243 |
| BceAI | ACGGC | 7 cut(s) | 86, 674, 1543, 1574, 1635, 1913, 1976 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1451, 1485 |
| BciVI | GTATCC | 2 cut(s) | 199, 501 |
| BcoDI | GTCTC | 4 cut(s) | 1250, 1936, 2126, 2241 |
| BfaI | CTAG | 7 cut(s) | 789, 828, 845, 899, 1085, 1223, 2096 |
| BfmI | CTRYAG | 1 cut(s) | 635 |
| BfoI | RGCGCY | 1 cut(s) | 584 |
| BfuAI | ACCTGC | 1 cut(s) | 1747 |
| BfuI | GTATCC | 2 cut(s) | 199, 501 |
| Bme18I | GGWCC | 6 cut(s) | 160, 300, 438, 724, 967, 1550 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1225 |
| BmeT110I | CYCGRG | 6 cut(s) | 398, 568, 1011, 1546, 1659, 1896 |
| BmgT120I | GGNCC | 9 cut(s) | 160, 300, 373, 438, 724, 839, 967, 1550, 1556 |
| BmrI | ACTGGG | 2 cut(s) | 318, 1422 |
| BmsI | GCATC | 6 cut(s) | 642, 1150, 1189, 1471, 1477, 1662 |
| BmuI | ACTGGG | 2 cut(s) | 318, 1422 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 1031, 1063, 1790, 1822 |
| BpmI | CTGGAG | 4 cut(s) | 1811, 1979, 2147, 2204 |
| Bpu10I | CCTNAGC | 1 cut(s) | 527 |
| BpuEI | CTTGAG | 1 cut(s) | 1874 |
| BsaAI | YACGTR | 1 cut(s) | 56 |
| BsaBI | GATNNNNATC | 2 cut(s) | 1722, 2253 |
| BsaHI | GRCGYC | 3 cut(s) | 1215, 1305, 1704 |
| BsaI | GGTCTC | 3 cut(s) | 1250, 2126, 2241 |
| BsaWI | WCCGGW | 4 cut(s) | 258, 944, 1517, 1594 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 31, 61, 1475, 1505, 1991, 2021 |
| Bse118I | RCCGGY | 6 cut(s) | 258, 944, 1381, 1517, 2171, 2228 |
| Bse1I | ACTGG | 7 cut(s) | 223, 313, 555, 1417, 1501, 2166, 2223 |
| Bse8I | GATNNNNATC | 2 cut(s) | 1722, 2253 |
| BseAI | TCCGGA | 1 cut(s) | 1594 |
| BseGI | GGATG | 7 cut(s) | 186, 547, 657, 1059, 1158, 1162, 2020 |
| BseJI | GATNNNNATC | 2 cut(s) | 1722, 2253 |
| BseMII | CTCAG | 4 cut(s) | 1401, 1809, 1860, 2252 |
| BseNI | ACTGG | 7 cut(s) | 223, 313, 555, 1417, 1501, 2166, 2223 |
| BseRI | GAGGAG | 5 cut(s) | 1057, 1157, 1748, 1895, 2012 |
| BseSI | GKGCMC | 2 cut(s) | 1395, 1681 |
| BseX3I | CGGCCG | 1 cut(s) | 2085 |
| BseXI | GCAGC | 9 cut(s) | 570, 633, 685, 949, 1339, 1849, 1903, 2023, 2026 |
| BsgI | GTGCAG | 2 cut(s) | 569, 1190 |
| Bsh1236I | CGCG | 6 cut(s) | 46, 765, 767, 1320, 1325, 1976 |
| Bsh1285I | CGRYCG | 3 cut(s) | 259, 1612, 2088 |
| BshNI | GGYRCC | 5 cut(s) | 167, 261, 947, 1514, 2091 |
| BshTI | ACCGGT | 3 cut(s) | 258, 944, 1517 |
| BsiEI | CGRYCG | 3 cut(s) | 259, 1612, 2088 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1049 |
| BsiHKCI | CYCGRG | 6 cut(s) | 398, 568, 1011, 1546, 1659, 1896 |
| BslFI | GGGAC | 8 cut(s) | 64, 146, 451, 737, 980, 1563, 1817, 1913 |
| BsmAI | GTCTC | 4 cut(s) | 1250, 1936, 2126, 2241 |
| BsmBI | CGTCTC | 1 cut(s) | 1936 |
| BsmFI | GGGAC | 8 cut(s) | 64, 146, 451, 737, 980, 1563, 1817, 1913 |
| BsmI | GAATGC | 2 cut(s) | 405, 691 |
| Bso31I | GGTCTC | 3 cut(s) | 1250, 2126, 2241 |
| BsoBI | CYCGRG | 6 cut(s) | 398, 568, 1011, 1546, 1659, 1896 |
| Bsp1286I | GDGCHC | 3 cut(s) | 1049, 1395, 1681 |
| Bsp13I | TCCGGA | 1 cut(s) | 1594 |
| Bsp143I | GATC | 8 cut(s) | 63, 212, 362, 1297, 1451, 1540, 1963, 2254 |
| Bsp19I | CCATGG | 1 cut(s) | 1335 |
| BspCNI | CTCAG | 4 cut(s) | 1400, 1810, 1861, 2251 |
| BspEI | TCCGGA | 1 cut(s) | 1594 |
| BspFNI | CGCG | 6 cut(s) | 46, 765, 767, 1320, 1325, 1976 |
| BspHI | TCATGA | 1 cut(s) | 2061 |
| BspMI | ACCTGC | 1 cut(s) | 1747 |
| BspPI | GGATC | 6 cut(s) | 58, 220, 370, 1292, 1446, 1971 |
| BspT107I | GGYRCC | 5 cut(s) | 167, 261, 947, 1514, 2091 |
| BspTNI | GGTCTC | 3 cut(s) | 1250, 2126, 2241 |
| BsrBI | CCGCTC | 1 cut(s) | 1801 |
| BsrFI | RCCGGY | 6 cut(s) | 258, 944, 1381, 1517, 2171, 2228 |
| BsrI | ACTGG | 7 cut(s) | 223, 313, 555, 1417, 1501, 2166, 2223 |
| BssAI | RCCGGY | 6 cut(s) | 258, 944, 1381, 1517, 2171, 2228 |
| BssMI | GATC | 8 cut(s) | 63, 212, 362, 1297, 1451, 1540, 1963, 2254 |
| BssNI | GRCGYC | 3 cut(s) | 1215, 1305, 1704 |
| BssSI | CACGAG | 3 cut(s) | 587, 1237, 1985 |
| BssT1I | CCWWGG | 2 cut(s) | 604, 1335 |
| Bst2BI | CACGAG | 3 cut(s) | 587, 1237, 1985 |
| Bst4CI | ACNGT | 6 cut(s) | 388, 442, 639, 783, 814, 1289 |
| Bst6I | CTCTTC | 4 cut(s) | 995, 1248, 1442, 1688 |
| BstACI | GRCGYC | 3 cut(s) | 1215, 1305, 1704 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 563, 1925 |
| BstBAI | YACGTR | 1 cut(s) | 56 |
| BstC8I | GCNNGC | 3 cut(s) | 1677, 1708, 2034 |
| BstDEI | CTNAG | 7 cut(s) | 527, 536, 1387, 1818, 1833, 1869, 2238 |
| BstDSI | CCRYGG | 3 cut(s) | 1335, 1974, 1992 |
| BstEII | GGTNACC | 1 cut(s) | 814 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 232 |
| BstF5I | GGATG | 7 cut(s) | 186, 547, 657, 1059, 1158, 1162, 2020 |
| BstFNI | CGCG | 6 cut(s) | 46, 765, 767, 1320, 1325, 1976 |
| BstH2I | RGCGCY | 1 cut(s) | 584 |
| BstHHI | GCGC | 4 cut(s) | 583, 767, 1322, 1327 |
| BstKTI | GATC | 8 cut(s) | 66, 215, 365, 1300, 1454, 1543, 1966, 2257 |
| BstMAI | GTCTC | 4 cut(s) | 1250, 1936, 2126, 2241 |
| BstMBI | GATC | 8 cut(s) | 63, 212, 362, 1297, 1451, 1540, 1963, 2254 |
| BstMCI | CGRYCG | 3 cut(s) | 259, 1612, 2088 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 79, 563, 652, 742, 1703, 1823, 1925, 2033 |
| BstNSI | RCATGY | 2 cut(s) | 493, 1180 |
| BstPI | GGTNACC | 1 cut(s) | 814 |
| BstSFI | CTRYAG | 1 cut(s) | 635 |
| BstSLI | GKGCMC | 2 cut(s) | 1395, 1681 |
| BstUI | CGCG | 6 cut(s) | 46, 765, 767, 1320, 1325, 1976 |
| BstV1I | GCAGC | 9 cut(s) | 570, 633, 685, 949, 1339, 1849, 1903, 2023, 2026 |
| BstX2I | RGATCY | 2 cut(s) | 362, 1451 |
| BstYI | RGATCY | 2 cut(s) | 362, 1451 |
| BstZI | CGGCCG | 1 cut(s) | 2085 |
| BsuI | GTATCC | 2 cut(s) | 199, 501 |
| BtgI | CCRYGG | 3 cut(s) | 1335, 1974, 1992 |
| BtgZI | GCGATG | 2 cut(s) | 462, 1434 |
| BtsCI | GGATG | 7 cut(s) | 186, 547, 657, 1059, 1158, 1162, 2020 |
| BtsIMutI | CAGTG | 6 cut(s) | 393, 1395, 1410, 1494, 2173, 2230 |
| BveI | ACCTGC | 1 cut(s) | 1747 |
| Cac8I | GCNNGC | 3 cut(s) | 1677, 1708, 2034 |
| CaiI | CAGNNNCTG | 1 cut(s) | 643 |
| CciI | TCATGA | 1 cut(s) | 2061 |
| CfoI | GCGC | 4 cut(s) | 583, 767, 1322, 1327 |
| Cfr10I | RCCGGY | 6 cut(s) | 258, 944, 1381, 1517, 2171, 2228 |
| Cfr13I | GGNCC | 9 cut(s) | 160, 300, 373, 438, 724, 839, 967, 1550, 1556 |
| Cfr42I | CCGCGG | 1 cut(s) | 1977 |
| Cfr9I | CCCGGG | 5 cut(s) | 568, 1011, 1546, 1659, 1896 |
| CseI | GACGC | 3 cut(s) | 524, 1313, 1712 |
| CspAI | ACCGGT | 3 cut(s) | 258, 944, 1517 |
| CviAII | CATG | 8 cut(s) | 466, 490, 718, 1177, 1336, 1772, 1935, 2062 |
| DdeI | CTNAG | 7 cut(s) | 527, 536, 1387, 1818, 1833, 1869, 2238 |
| DpnI | GATC | 8 cut(s) | 65, 214, 364, 1299, 1453, 1542, 1965, 2256 |
| DpnII | GATC | 8 cut(s) | 63, 212, 362, 1297, 1451, 1540, 1963, 2254 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1225 |
| EaeI | YGGCCR | 9 cut(s) | 390, 552, 714, 1338, 1498, 1824, 2085, 2157, 2214 |
| EagI | CGGCCG | 1 cut(s) | 2085 |
| Eam1104I | CTCTTC | 4 cut(s) | 995, 1248, 1442, 1688 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1225 |
| EarI | CTCTTC | 4 cut(s) | 995, 1248, 1442, 1688 |
| Ecl136II | GAGCTC | 1 cut(s) | 1047 |
| EclXI | CGGCCG | 1 cut(s) | 2085 |
| Eco130I | CCWWGG | 2 cut(s) | 604, 1335 |
| Eco147I | AGGCCT | 1 cut(s) | 532 |
| Eco24I | GRGCYC | 1 cut(s) | 1049 |
| Eco31I | GGTCTC | 3 cut(s) | 1250, 2126, 2241 |
| Eco47I | GGWCC | 6 cut(s) | 160, 300, 438, 724, 967, 1550 |
| Eco52I | CGGCCG | 1 cut(s) | 2085 |
| Eco53kI | GAGCTC | 1 cut(s) | 1047 |
| Eco57I | CTGAAG | 2 cut(s) | 518, 1748 |
| Eco72I | CACGTG | 1 cut(s) | 56 |
| Eco88I | CYCGRG | 6 cut(s) | 398, 568, 1011, 1546, 1659, 1896 |
| Eco91I | GGTNACC | 1 cut(s) | 814 |
| EcoICRI | GAGCTC | 1 cut(s) | 1047 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 232 |
| EcoO109I | RGGNCCY | 4 cut(s) | 160, 300, 373, 724 |
| EcoO65I | GGTNACC | 1 cut(s) | 814 |
| EcoT14I | CCWWGG | 2 cut(s) | 604, 1335 |
| EcoT38I | GRGCYC | 1 cut(s) | 1049 |
| ErhI | CCWWGG | 2 cut(s) | 604, 1335 |
| Esp3I | CGTCTC | 1 cut(s) | 1936 |
| FaeI | CATG | 8 cut(s) | 469, 493, 721, 1180, 1339, 1775, 1938, 2065 |
| FaqI | GGGAC | 8 cut(s) | 64, 146, 451, 737, 980, 1563, 1817, 1913 |
| FatI | CATG | 8 cut(s) | 465, 489, 717, 1176, 1335, 1771, 1934, 2061 |
| FauI | CCCGC | 8 cut(s) | 799, 1325, 1594, 1668, 1794, 1869, 1981, 2109 |
| FblI | GTMKAC | 4 cut(s) | 276, 486, 634, 1218 |
| FokI | GGATG | 7 cut(s) | 193, 554, 664, 1046, 1165, 1169, 2027 |
| FriOI | GRGCYC | 1 cut(s) | 1049 |
| FspBI | CTAG | 7 cut(s) | 789, 828, 845, 899, 1085, 1223, 2096 |
| GlaI | GCGC | 4 cut(s) | 582, 766, 1321, 1326 |
| GsuI | CTGGAG | 4 cut(s) | 1811, 1979, 2147, 2204 |
| HaeII | RGCGCY | 1 cut(s) | 584 |
| HgaI | GACGC | 3 cut(s) | 524, 1313, 1712 |
| HhaI | GCGC | 4 cut(s) | 583, 767, 1322, 1327 |
| Hin1I | GRCGYC | 3 cut(s) | 1215, 1305, 1704 |
| Hin1II | CATG | 8 cut(s) | 469, 493, 721, 1180, 1339, 1775, 1938, 2065 |
| Hin6I | GCGC | 4 cut(s) | 581, 765, 1320, 1325 |
| HinP1I | GCGC | 4 cut(s) | 581, 765, 1320, 1325 |
| HincII | GTYRAC | 1 cut(s) | 1219 |
| HindII | GTYRAC | 1 cut(s) | 1219 |
| HinfI | GANTC | 8 cut(s) | 185, 335, 592, 1645, 1664, 1805, 1901, 1952 |
| HphI | GGTGA | 9 cut(s) | 359, 657, 826, 1301, 1451, 1498, 1535, 1771, 1871 |
| Hpy166II | GTNNAC | 9 cut(s) | 277, 423, 487, 635, 772, 1219, 1292, 1494, 1693 |
| Hpy188I | TCNGA | 9 cut(s) | 190, 322, 411, 988, 1033, 1728, 1906, 2014, 2241 |
| Hpy188III | TCNNGA | 8 cut(s) | 30, 140, 398, 1301, 1595, 1888, 1958, 2062 |
| Hpy8I | GTNNAC | 9 cut(s) | 277, 423, 487, 635, 772, 1219, 1292, 1494, 1693 |
| Hpy99I | CGWCG | 7 cut(s) | 96, 220, 518, 1217, 1220, 1564, 1706 |
| HpyCH4III | ACNGT | 6 cut(s) | 388, 442, 639, 783, 814, 1289 |
| HpyCH4IV | ACGT | 3 cut(s) | 55, 1215, 1490 |
| HpyCH4V | TGCA | 5 cut(s) | 586, 705, 1207, 1523, 1919 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 79, 563, 652, 742, 1703, 1823, 1925, 2033 |
| HpyF3I | CTNAG | 7 cut(s) | 527, 536, 1387, 1818, 1833, 1869, 2238 |
| HpySE526I | ACGT | 3 cut(s) | 55, 1215, 1490 |
| Hsp92I | GRCGYC | 3 cut(s) | 1215, 1305, 1704 |
| Hsp92II | CATG | 8 cut(s) | 469, 493, 721, 1180, 1339, 1775, 1938, 2065 |
| HspAI | GCGC | 4 cut(s) | 581, 765, 1320, 1325 |
| Kpn2I | TCCGGA | 1 cut(s) | 1594 |
| KpnI | GGTACC | 5 cut(s) | 171, 265, 951, 1518, 2095 |
| KspI | CCGCGG | 1 cut(s) | 1977 |
| Kzo9I | GATC | 8 cut(s) | 63, 212, 362, 1297, 1451, 1540, 1963, 2254 |
| LmnI | GCTCC | 8 cut(s) | 603, 1044, 1104, 1416, 1798, 1849, 2143, 2200 |
| Lsp1109I | GCAGC | 9 cut(s) | 570, 633, 685, 949, 1339, 1849, 1903, 2023, 2026 |
| LweI | GCATC | 6 cut(s) | 642, 1150, 1189, 1471, 1477, 1662 |
| MaeI | CTAG | 7 cut(s) | 789, 828, 845, 899, 1085, 1223, 2096 |
| MaeII | ACGT | 3 cut(s) | 55, 1215, 1490 |
| MaeIII | GTNAC | 8 cut(s) | 382, 615, 663, 814, 847, 1312, 1486, 1864 |
| MalI | GATC | 8 cut(s) | 65, 214, 364, 1299, 1453, 1542, 1965, 2256 |
| MbiI | CCGCTC | 1 cut(s) | 1801 |
| MboI | GATC | 8 cut(s) | 63, 212, 362, 1297, 1451, 1540, 1963, 2254 |
| MflI | RGATCY | 2 cut(s) | 362, 1451 |
| MhlI | GDGCHC | 3 cut(s) | 1049, 1395, 1681 |
| MlsI | TGGCCA | 3 cut(s) | 392, 716, 1826 |
| MluCI | AATT | 5 cut(s) | 134, 281, 1166, 1269, 2108 |
| MluI | ACGCGT | 1 cut(s) | 44 |
| MluNI | TGGCCA | 3 cut(s) | 392, 716, 1826 |
| MlyI | GAGTC | 5 cut(s) | 344, 1673, 1799, 1895, 1946 |
| MmeI | TCCRAC | 1 cut(s) | 1245 |
| Mox20I | TGGCCA | 3 cut(s) | 392, 716, 1826 |
| MroI | TCCGGA | 1 cut(s) | 1594 |
| MscI | TGGCCA | 3 cut(s) | 392, 716, 1826 |
| MseI | TTAA | 2 cut(s) | 1169, 2070 |
| MslI | CAYNNNNRTG | 3 cut(s) | 470, 1181, 1367 |
| Msp20I | TGGCCA | 3 cut(s) | 392, 716, 1826 |
| MspA1I | CMGCKG | 3 cut(s) | 940, 1840, 1976 |
| MteI | GCGCNGCGC | 1 cut(s) | 1323 |
| Mva1269I | GAATGC | 2 cut(s) | 405, 691 |
| MvnI | CGCG | 6 cut(s) | 46, 765, 767, 1320, 1325, 1976 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 79, 563, 652, 742, 1703, 1823, 1925, 2033 |
| NcoI | CCATGG | 1 cut(s) | 1335 |
| NdeII | GATC | 8 cut(s) | 63, 212, 362, 1297, 1451, 1540, 1963, 2254 |
| NlaIII | CATG | 8 cut(s) | 469, 493, 721, 1180, 1339, 1775, 1938, 2065 |
| NmeAIII | GCCGAG | 3 cut(s) | 46, 441, 1526 |
| NmuCI | GTSAC | 4 cut(s) | 663, 814, 1312, 1486 |
| NspI | RCATGY | 2 cut(s) | 493, 1180 |
| OliI | CACNNNNGTG | 1 cut(s) | 1367 |
| PagI | TCATGA | 1 cut(s) | 2061 |
| PaqCI | CACCTGC | 1 cut(s) | 1747 |
| PceI | AGGCCT | 1 cut(s) | 532 |
| PcsI | WCGNNNNNNNCGW | 4 cut(s) | 91, 215, 302, 1707 |
| PctI | GAATGC | 2 cut(s) | 405, 691 |
| PfeI | GAWTC | 3 cut(s) | 185, 592, 1645 |
| PfoI | TCCNGGA | 2 cut(s) | 2124, 2181 |
| PinAI | ACCGGT | 3 cut(s) | 258, 944, 1517 |
| PleI | GAGTC | 5 cut(s) | 343, 1672, 1799, 1895, 1946 |
| PmaCI | CACGTG | 1 cut(s) | 56 |
| PmlI | CACGTG | 1 cut(s) | 56 |
| PpsI | GAGTC | 5 cut(s) | 343, 1672, 1799, 1895, 1946 |
| Ppu21I | YACGTR | 1 cut(s) | 56 |
| PpuMI | RGGWCCY | 3 cut(s) | 160, 300, 724 |
| PshBI | ATTAAT | 1 cut(s) | 1169 |
| Psp124BI | GAGCTC | 1 cut(s) | 1049 |
| Psp5II | RGGWCCY | 3 cut(s) | 160, 300, 724 |
| PspCI | CACGTG | 1 cut(s) | 56 |
| PspEI | GGTNACC | 1 cut(s) | 814 |
| PspPI | GGNCC | 9 cut(s) | 160, 300, 373, 438, 724, 839, 967, 1550, 1556 |
| PspPPI | RGGWCCY | 3 cut(s) | 160, 300, 724 |
| PstNI | CAGNNNCTG | 1 cut(s) | 643 |
| PsuI | RGATCY | 2 cut(s) | 362, 1451 |
| PvuII | CAGCTG | 1 cut(s) | 940 |
| RseI | CAYNNNNRTG | 3 cut(s) | 470, 1181, 1367 |
| SacI | GAGCTC | 1 cut(s) | 1049 |
| SacII | CCGCGG | 1 cut(s) | 1977 |
| SalI | GTCGAC | 1 cut(s) | 1217 |
| SaqAI | TTAA | 2 cut(s) | 1169, 2070 |
| Sau3AI | GATC | 8 cut(s) | 63, 212, 362, 1297, 1451, 1540, 1963, 2254 |
| Sau96I | GGNCC | 9 cut(s) | 160, 300, 373, 438, 724, 839, 967, 1550, 1556 |
| SchI | GAGTC | 5 cut(s) | 344, 1673, 1799, 1895, 1946 |
| SduI | GDGCHC | 3 cut(s) | 1049, 1395, 1681 |
| SfaNI | GCATC | 6 cut(s) | 642, 1150, 1189, 1471, 1477, 1662 |
| SfcI | CTRYAG | 1 cut(s) | 635 |
| Sfr303I | CCGCGG | 1 cut(s) | 1977 |
| SgrBI | CCGCGG | 1 cut(s) | 1977 |
| SinI | GGWCC | 6 cut(s) | 160, 300, 438, 724, 967, 1550 |
| SmaI | CCCGGG | 5 cut(s) | 570, 1013, 1548, 1661, 1898 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 470, 1181, 1367 |
| SmlI | CTYRAG | 1 cut(s) | 1853 |
| SmoI | CTYRAG | 1 cut(s) | 1853 |
| Sse9I | AATT | 5 cut(s) | 134, 281, 1166, 1269, 2108 |
| SseBI | AGGCCT | 1 cut(s) | 532 |
| SspMI | CTAG | 7 cut(s) | 789, 828, 845, 899, 1085, 1223, 2096 |
| SstI | GAGCTC | 1 cut(s) | 1049 |
| StuI | AGGCCT | 1 cut(s) | 532 |
| StyI | CCWWGG | 2 cut(s) | 604, 1335 |
| TaaI | ACNGT | 6 cut(s) | 388, 442, 639, 783, 814, 1289 |
| TaiI | ACGT | 3 cut(s) | 58, 1218, 1493 |
| TasI | AATT | 5 cut(s) | 134, 281, 1166, 1269, 2108 |
| TatI | WGTACW | 1 cut(s) | 2001 |
| TfiI | GAWTC | 3 cut(s) | 185, 592, 1645 |
| Tru1I | TTAA | 2 cut(s) | 1169, 2070 |
| Tru9I | TTAA | 2 cut(s) | 1169, 2070 |
| TscAI | CASTG | 6 cut(s) | 393, 1395, 1417, 1501, 2173, 2230 |
| TseFI | GTSAC | 4 cut(s) | 663, 814, 1312, 1486 |
| TseI | GCWGC | 9 cut(s) | 583, 646, 673, 937, 1327, 1837, 1916, 2036, 2039 |
| Tsp45I | GTSAC | 4 cut(s) | 663, 814, 1312, 1486 |
| TspDTI | ATGAA | 7 cut(s) | 465, 488, 683, 1048, 1760, 2078, 2115 |
| TspMI | CCCGGG | 5 cut(s) | 568, 1011, 1546, 1659, 1896 |
| TspRI | CASTG | 6 cut(s) | 393, 1395, 1417, 1501, 2173, 2230 |
| VpaK11BI | GGWCC | 6 cut(s) | 160, 300, 438, 724, 967, 1550 |
| VspI | ATTAAT | 1 cut(s) | 1169 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 232 |
| XapI | RAATTY | 2 cut(s) | 134, 2108 |
| XceI | RCATGY | 2 cut(s) | 493, 1180 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 178 |
| XmaI | CCCGGG | 5 cut(s) | 568, 1011, 1546, 1659, 1896 |
| XmiI | GTMKAC | 4 cut(s) | 276, 486, 634, 1218 |
| XspI | CTAG | 7 cut(s) | 789, 828, 845, 899, 1085, 1223, 2096 |
| ZraI | GACGTC | 1 cut(s) | 1216 |