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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 687 |
| AccBSI | CCGCTC | 2 cut(s) | 527, 578 |
| AccII | CGCG | 2 cut(s) | 198, 681 |
| AccIII | TCCGGA | 1 cut(s) | 375 |
| AciI | CCGC | 4 cut(s) | 198, 300, 527, 578 |
| AclWI | GGATC | 3 cut(s) | 110, 605, 612 |
| AcyI | GRCGYC | 1 cut(s) | 684 |
| AfaI | GTAC | 2 cut(s) | 104, 204 |
| AfeI | AGCGCT | 1 cut(s) | 649 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 154 |
| AgeI | ACCGGT | 1 cut(s) | 326 |
| AgsI | TTSAA | 2 cut(s) | 49, 539 |
| AluBI | AGCT | 1 cut(s) | 671 |
| AluI | AGCT | 1 cut(s) | 671 |
| Alw26I | GTCTC | 2 cut(s) | 478, 648 |
| AlwI | GGATC | 3 cut(s) | 110, 605, 612 |
| Ama87I | CYCGRG | 3 cut(s) | 135, 477, 651 |
| Aor13HI | TCCGGA | 1 cut(s) | 375 |
| Aor51HI | AGCGCT | 1 cut(s) | 649 |
| AoxI | GGCC | 1 cut(s) | 694 |
| ApeKI | GCWGC | 2 cut(s) | 584, 671 |
| AsiGI | ACCGGT | 1 cut(s) | 326 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 722 |
| AspLEI | GCGC | 3 cut(s) | 6, 198, 650 |
| AspS9I | GGNCC | 1 cut(s) | 378 |
| AsuHPI | GGTGA | 2 cut(s) | 626, 652 |
| AvaI | CYCGRG | 3 cut(s) | 135, 477, 651 |
| AvaII | GGWCC | 1 cut(s) | 378 |
| BanII | GRGCYC | 1 cut(s) | 407 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 240, 272 |
| BbsI | GAAGAC | 1 cut(s) | 715 |
| BbvI | GCAGC | 2 cut(s) | 596, 658 |
| BccI | CCATC | 2 cut(s) | 300, 450 |
| BceAI | ACGGC | 1 cut(s) | 221 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 275, 309 |
| BcoDI | GTCTC | 2 cut(s) | 478, 648 |
| BfaI | CTAG | 2 cut(s) | 261, 630 |
| BfmI | CTRYAG | 2 cut(s) | 280, 424 |
| BfoI | RGCGCY | 1 cut(s) | 651 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 357 |
| BisI | GCNGC | 2 cut(s) | 585, 672 |
| BlsI | GCNGC | 2 cut(s) | 586, 673 |
| Bme18I | GGWCC | 1 cut(s) | 378 |
| BmeT110I | CYCGRG | 3 cut(s) | 135, 477, 651 |
| BmgT120I | GGNCC | 1 cut(s) | 378 |
| BmiI | GGNNCC | 2 cut(s) | 110, 350 |
| BmsI | GCATC | 1 cut(s) | 7 |
| BpiI | GAAGAC | 1 cut(s) | 715 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 516, 548 |
| BpmI | CTGGAG | 1 cut(s) | 537 |
| BpuEI | CTTGAG | 1 cut(s) | 104 |
| BsaBI | GATNNNNATC | 1 cut(s) | 36 |
| BsaHI | GRCGYC | 1 cut(s) | 684 |
| BsaJI | CCNNGG | 1 cut(s) | 7 |
| BsaWI | WCCGGW | 2 cut(s) | 326, 375 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 154 |
| Bse118I | RCCGGY | 2 cut(s) | 326, 392 |
| Bse8I | GATNNNNATC | 1 cut(s) | 36 |
| BseAI | TCCGGA | 1 cut(s) | 375 |
| BseDI | CCNNGG | 1 cut(s) | 7 |
| BseGI | GGATG | 1 cut(s) | 640 |
| BseJI | GATNNNNATC | 1 cut(s) | 36 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 154 |
| BseMII | CTCAG | 1 cut(s) | 447 |
| BseRI | GAGGAG | 1 cut(s) | 475 |
| BseXI | GCAGC | 2 cut(s) | 596, 658 |
| Bsh1236I | CGCG | 2 cut(s) | 198, 681 |
| BshFI | GGCC | 1 cut(s) | 696 |
| BshTI | ACCGGT | 1 cut(s) | 326 |
| BsiHKCI | CYCGRG | 3 cut(s) | 135, 477, 651 |
| BsiSI | CCGG | 4 cut(s) | 327, 376, 393, 697 |
| BslFI | GGGAC | 3 cut(s) | 172, 414, 543 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 154 |
| BsmAI | GTCTC | 2 cut(s) | 478, 648 |
| BsmFI | GGGAC | 3 cut(s) | 172, 414, 543 |
| BsnI | GGCC | 1 cut(s) | 696 |
| BsoBI | CYCGRG | 3 cut(s) | 135, 477, 651 |
| Bsp1286I | GDGCHC | 1 cut(s) | 407 |
| Bsp13I | TCCGGA | 1 cut(s) | 375 |
| Bsp143I | GATC | 4 cut(s) | 37, 115, 597, 604 |
| Bsp68I | TCGCGA | 1 cut(s) | 681 |
| BspACI | CCGC | 4 cut(s) | 198, 300, 527, 578 |
| BspANI | GGCC | 1 cut(s) | 696 |
| BspCNI | CTCAG | 1 cut(s) | 448 |
| BspEI | TCCGGA | 1 cut(s) | 375 |
| BspFNI | CGCG | 2 cut(s) | 198, 681 |
| BspLI | GGNNCC | 2 cut(s) | 110, 350 |
| BspPI | GGATC | 3 cut(s) | 110, 605, 612 |
| BspQI | GCTCTTC | 1 cut(s) | 638 |
| BsrBI | CCGCTC | 2 cut(s) | 527, 578 |
| BsrFI | RCCGGY | 2 cut(s) | 326, 392 |
| BssAI | RCCGGY | 2 cut(s) | 326, 392 |
| BssECI | CCNNGG | 1 cut(s) | 7 |
| BssMI | GATC | 4 cut(s) | 37, 115, 597, 604 |
| BssNI | GRCGYC | 1 cut(s) | 684 |
| BssT1I | CCWWGG | 1 cut(s) | 7 |
| Bst6I | CTCTTC | 3 cut(s) | 180, 266, 638 |
| BstACI | GRCGYC | 1 cut(s) | 684 |
| BstDEI | CTNAG | 1 cut(s) | 456 |
| BstF5I | GGATG | 1 cut(s) | 640 |
| BstFNI | CGCG | 2 cut(s) | 198, 681 |
| BstH2I | RGCGCY | 1 cut(s) | 651 |
| BstHHI | GCGC | 3 cut(s) | 6, 198, 650 |
| BstKTI | GATC | 4 cut(s) | 40, 118, 600, 607 |
| BstMAI | GTCTC | 2 cut(s) | 478, 648 |
| BstMBI | GATC | 4 cut(s) | 37, 115, 597, 604 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 283, 357, 446, 584 |
| BstSFI | CTRYAG | 2 cut(s) | 280, 424 |
| BstUI | CGCG | 2 cut(s) | 198, 681 |
| BstV1I | GCAGC | 2 cut(s) | 596, 658 |
| BstV2I | GAAGAC | 1 cut(s) | 715 |
| BstX2I | RGATCY | 1 cut(s) | 604 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 236 |
| BstYI | RGATCY | 1 cut(s) | 604 |
| BsuRI | GGCC | 1 cut(s) | 696 |
| BtsCI | GGATG | 1 cut(s) | 640 |
| BtuMI | TCGCGA | 1 cut(s) | 681 |
| CfoI | GCGC | 3 cut(s) | 6, 198, 650 |
| Cfr10I | RCCGGY | 2 cut(s) | 326, 392 |
| Cfr13I | GGNCC | 1 cut(s) | 378 |
| CpoI | CGGWCCG | 1 cut(s) | 378 |
| CseI | GACGC | 1 cut(s) | 376 |
| Csp6I | GTAC | 2 cut(s) | 103, 203 |
| CspAI | ACCGGT | 1 cut(s) | 326 |
| CspI | CGGWCCG | 1 cut(s) | 378 |
| CviAII | CATG | 1 cut(s) | 688 |
| CviQI | GTAC | 2 cut(s) | 103, 203 |
| DdeI | CTNAG | 1 cut(s) | 456 |
| DpnI | GATC | 4 cut(s) | 39, 117, 599, 606 |
| DpnII | GATC | 4 cut(s) | 37, 115, 597, 604 |
| Eam1104I | CTCTTC | 3 cut(s) | 180, 266, 638 |
| EarI | CTCTTC | 3 cut(s) | 180, 266, 638 |
| Eco130I | CCWWGG | 1 cut(s) | 7 |
| Eco24I | GRGCYC | 1 cut(s) | 407 |
| Eco32I | GATATC | 1 cut(s) | 180 |
| Eco47I | GGWCC | 1 cut(s) | 378 |
| Eco47III | AGCGCT | 1 cut(s) | 649 |
| Eco88I | CYCGRG | 3 cut(s) | 135, 477, 651 |
| EcoRV | GATATC | 1 cut(s) | 180 |
| EcoT14I | CCWWGG | 1 cut(s) | 7 |
| EcoT38I | GRGCYC | 1 cut(s) | 407 |
| ErhI | CCWWGG | 1 cut(s) | 7 |
| FaeI | CATG | 1 cut(s) | 691 |
| FaiI | YATR | 7 cut(s) | 27, 56, 213, 282, 426, 567, 689 |
| FaqI | GGGAC | 3 cut(s) | 172, 414, 543 |
| FatI | CATG | 1 cut(s) | 687 |
| FauI | CCCGC | 3 cut(s) | 293, 520, 571 |
| Fnu4HI | GCNGC | 2 cut(s) | 585, 672 |
| FokI | GGATG | 1 cut(s) | 647 |
| FriOI | GRGCYC | 1 cut(s) | 407 |
| Fsp4HI | GCNGC | 2 cut(s) | 585, 672 |
| FspBI | CTAG | 2 cut(s) | 261, 630 |
| GlaI | GCGC | 3 cut(s) | 5, 197, 649 |
| GluI | GCNGC | 2 cut(s) | 585, 672 |
| GsuI | CTGGAG | 1 cut(s) | 537 |
| HaeII | RGCGCY | 1 cut(s) | 651 |
| HaeIII | GGCC | 1 cut(s) | 696 |
| HapII | CCGG | 4 cut(s) | 327, 376, 393, 697 |
| HgaI | GACGC | 1 cut(s) | 376 |
| HhaI | GCGC | 3 cut(s) | 6, 198, 650 |
| Hin1I | GRCGYC | 1 cut(s) | 684 |
| Hin1II | CATG | 1 cut(s) | 691 |
| Hin6I | GCGC | 3 cut(s) | 4, 196, 648 |
| HinP1I | GCGC | 3 cut(s) | 4, 196, 648 |
| HinfI | GANTC | 4 cut(s) | 419, 531, 552, 676 |
| HpaII | CCGG | 4 cut(s) | 327, 376, 393, 697 |
| HphI | GGTGA | 2 cut(s) | 626, 652 |
| Hpy166II | GTNNAC | 1 cut(s) | 203 |
| Hpy188I | TCNGA | 1 cut(s) | 184 |
| Hpy188III | TCNNGA | 5 cut(s) | 376, 479, 608, 653, 680 |
| Hpy8I | GTNNAC | 1 cut(s) | 203 |
| Hpy99I | CGWCG | 3 cut(s) | 101, 392, 686 |
| HpyAV | CCTTC | 6 cut(s) | 43, 500, 515, 605, 658, 686 |
| HpyCH4IV | ACGT | 5 cut(s) | 161, 315, 336, 684, 720 |
| HpyCH4V | TGCA | 1 cut(s) | 25 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 283, 357, 446, 584 |
| HpyF3I | CTNAG | 1 cut(s) | 456 |
| HpySE526I | ACGT | 5 cut(s) | 161, 315, 336, 684, 720 |
| Hsp92I | GRCGYC | 1 cut(s) | 684 |
| Hsp92II | CATG | 1 cut(s) | 691 |
| HspAI | GCGC | 3 cut(s) | 4, 196, 648 |
| Kpn2I | TCCGGA | 1 cut(s) | 375 |
| Kzo9I | GATC | 4 cut(s) | 37, 115, 597, 604 |
| LguI | GCTCTTC | 1 cut(s) | 638 |
| LmnI | GCTCC | 5 cut(s) | 240, 348, 524, 581, 699 |
| LpnPI | CCDG | 8 cut(s) | 35, 141, 157, 340, 389, 406, 501, 710 |
| Lsp1109I | GCAGC | 2 cut(s) | 596, 658 |
| LweI | GCATC | 1 cut(s) | 7 |
| MaeI | CTAG | 2 cut(s) | 261, 630 |
| MaeII | ACGT | 5 cut(s) | 161, 315, 336, 684, 720 |
| MaeIII | GTNAC | 4 cut(s) | 130, 165, 316, 569 |
| MalI | GATC | 4 cut(s) | 39, 117, 599, 606 |
| MbiI | CCGCTC | 2 cut(s) | 527, 578 |
| MboI | GATC | 4 cut(s) | 37, 115, 597, 604 |
| MfeI | CAATTG | 1 cut(s) | 450 |
| MflI | RGATCY | 1 cut(s) | 604 |
| MhlI | GDGCHC | 1 cut(s) | 407 |
| MluCI | AATT | 1 cut(s) | 450 |
| MlyI | GAGTC | 3 cut(s) | 525, 561, 685 |
| MroI | TCCGGA | 1 cut(s) | 375 |
| MroXI | GAANNNNTTC | 1 cut(s) | 722 |
| MseI | TTAA | 2 cut(s) | 339, 558 |
| MslI | CAYNNNNRTG | 1 cut(s) | 30 |
| MspI | CCGG | 4 cut(s) | 327, 376, 393, 697 |
| MunI | CAATTG | 1 cut(s) | 450 |
| MvnI | CGCG | 2 cut(s) | 198, 681 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 283, 357, 446, 584 |
| NdeII | GATC | 4 cut(s) | 37, 115, 597, 604 |
| NlaIII | CATG | 1 cut(s) | 691 |
| NlaIV | GGNNCC | 2 cut(s) | 110, 350 |
| NmeAIII | GCCGAG | 1 cut(s) | 393 |
| NmuCI | GTSAC | 2 cut(s) | 165, 569 |
| NruI | TCGCGA | 1 cut(s) | 681 |
| PaeR7I | CTCGAG | 1 cut(s) | 651 |
| PciSI | GCTCTTC | 1 cut(s) | 638 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 111, 240, 606 |
| PdmI | GAANNNNTTC | 1 cut(s) | 722 |
| PfeI | GAWTC | 1 cut(s) | 419 |
| PflFI | GACNNNGTC | 1 cut(s) | 163 |
| PinAI | ACCGGT | 1 cut(s) | 326 |
| PkrI | GCNGC | 2 cut(s) | 586, 673 |
| PleI | GAGTC | 3 cut(s) | 525, 560, 684 |
| PpsI | GAGTC | 3 cut(s) | 525, 560, 684 |
| PspN4I | GGNNCC | 2 cut(s) | 110, 350 |
| PspPI | GGNCC | 1 cut(s) | 378 |
| PsuI | RGATCY | 1 cut(s) | 604 |
| PsyI | GACNNNGTC | 1 cut(s) | 163 |
| RruI | TCGCGA | 1 cut(s) | 681 |
| RsaI | GTAC | 2 cut(s) | 104, 204 |
| RsaNI | GTAC | 2 cut(s) | 103, 203 |
| RseI | CAYNNNNRTG | 1 cut(s) | 30 |
| Rsr2I | CGGWCCG | 1 cut(s) | 378 |
| RsrII | CGGWCCG | 1 cut(s) | 378 |
| SapI | GCTCTTC | 1 cut(s) | 638 |
| SaqAI | TTAA | 2 cut(s) | 339, 558 |
| SatI | GCNGC | 2 cut(s) | 585, 672 |
| Sau3AI | GATC | 4 cut(s) | 37, 115, 597, 604 |
| Sau96I | GGNCC | 1 cut(s) | 378 |
| SchI | GAGTC | 3 cut(s) | 525, 561, 685 |
| SduI | GDGCHC | 1 cut(s) | 407 |
| SfaNI | GCATC | 1 cut(s) | 7 |
| SfcI | CTRYAG | 2 cut(s) | 280, 424 |
| Sfr274I | CTCGAG | 1 cut(s) | 651 |
| SinI | GGWCC | 1 cut(s) | 378 |
| SlaI | CTCGAG | 1 cut(s) | 651 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 30 |
| SmlI | CTYRAG | 2 cut(s) | 83, 651 |
| SmoI | CTYRAG | 2 cut(s) | 83, 651 |
| Sse9I | AATT | 1 cut(s) | 450 |
| SsiI | CCGC | 4 cut(s) | 198, 300, 527, 578 |
| SspMI | CTAG | 2 cut(s) | 261, 630 |
| StyI | CCWWGG | 1 cut(s) | 7 |
| TaiI | ACGT | 5 cut(s) | 164, 318, 339, 687, 723 |
| TaqI | TCGA | 4 cut(s) | 36, 609, 624, 652 |
| TaqII | GACCGA | 2 cut(s) | 395, 588 |
| TasI | AATT | 1 cut(s) | 450 |
| TfiI | GAWTC | 1 cut(s) | 419 |
| Tru1I | TTAA | 2 cut(s) | 339, 558 |
| Tru9I | TTAA | 2 cut(s) | 339, 558 |
| TseFI | GTSAC | 2 cut(s) | 165, 569 |
| TseI | GCWGC | 2 cut(s) | 584, 671 |
| Tsp45I | GTSAC | 2 cut(s) | 165, 569 |
| TspDTI | ATGAA | 1 cut(s) | 87 |
| TspGWI | ACGGA | 2 cut(s) | 610, 727 |
| Tth111I | GACNNNGTC | 1 cut(s) | 163 |
| VpaK11BI | GGWCC | 1 cut(s) | 378 |
| XhoI | CTCGAG | 1 cut(s) | 651 |
| XmnI | GAANNNNTTC | 1 cut(s) | 722 |
| XspI | CTAG | 2 cut(s) | 261, 630 |
| ZraI | GACGTC | 1 cut(s) | 685 |