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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 664 |
| AccB1I | GGYRCC | 1 cut(s) | 664 |
| AccBSI | CCGCTC | 2 cut(s) | 661, 701 |
| AciI | CCGC | 4 cut(s) | 282, 352, 661, 701 |
| AclWI | GGATC | 2 cut(s) | 113, 399 |
| AcsI | RAATTY | 1 cut(s) | 425 |
| AcuI | CTGAAG | 1 cut(s) | 608 |
| AfaI | GTAC | 3 cut(s) | 407, 626, 666 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 29, 358, 478, 484, 589, 592 |
| AflIII | ACRYGT | 1 cut(s) | 72 |
| AgsI | TTSAA | 1 cut(s) | 149 |
| AjiI | CACGTC | 1 cut(s) | 112 |
| AjnI | CCWGG | 3 cut(s) | 313, 465, 477 |
| AluBI | AGCT | 2 cut(s) | 649, 689 |
| AluI | AGCT | 2 cut(s) | 649, 689 |
| Alw26I | GTCTC | 3 cut(s) | 208, 267, 291 |
| AlwI | GGATC | 2 cut(s) | 113, 399 |
| AoxI | GGCC | 1 cut(s) | 480 |
| ApeKI | GCWGC | 1 cut(s) | 603 |
| ApoI | RAATTY | 1 cut(s) | 425 |
| Asp718I | GGTACC | 1 cut(s) | 664 |
| AspLEI | GCGC | 1 cut(s) | 541 |
| AspS9I | GGNCC | 3 cut(s) | 193, 448, 469 |
| AsuC2I | CCSGG | 2 cut(s) | 259, 613 |
| AsuHPI | GGTGA | 3 cut(s) | 341, 385, 610 |
| AvaII | GGWCC | 3 cut(s) | 193, 448, 469 |
| BanI | GGYRCC | 1 cut(s) | 664 |
| BbvI | GCAGC | 1 cut(s) | 615 |
| BccI | CCATC | 2 cut(s) | 61, 566 |
| BciT130I | CCWGG | 3 cut(s) | 315, 467, 479 |
| BciVI | GTATCC | 1 cut(s) | 571 |
| BcnI | CCSGG | 2 cut(s) | 259, 613 |
| BcoDI | GTCTC | 3 cut(s) | 208, 267, 291 |
| BfuI | GTATCC | 1 cut(s) | 571 |
| BisI | GCNGC | 1 cut(s) | 604 |
| BlsI | GCNGC | 1 cut(s) | 605 |
| Bme1390I | CCNGG | 5 cut(s) | 259, 315, 467, 479, 613 |
| Bme18I | GGWCC | 3 cut(s) | 193, 448, 469 |
| BmgBI | CACGTC | 1 cut(s) | 112 |
| BmgT120I | GGNCC | 3 cut(s) | 193, 448, 469 |
| BmiI | GGNNCC | 5 cut(s) | 194, 195, 256, 449, 666 |
| BmrFI | CCNGG | 5 cut(s) | 259, 315, 467, 479, 613 |
| BmrI | ACTGGG | 1 cut(s) | 393 |
| BmsI | GCATC | 2 cut(s) | 429, 522 |
| BmuI | ACTGGG | 1 cut(s) | 393 |
| BplI | GAGNNNNNCTC | 1 cut(s) | 690 |
| BpmI | CTGGAG | 3 cut(s) | 182, 671, 711 |
| Bpu10I | CCTNAGC | 1 cut(s) | 443 |
| BpuEI | CTTGAG | 1 cut(s) | 437 |
| BpuMI | CCSGG | 2 cut(s) | 259, 613 |
| BsaI | GGTCTC | 2 cut(s) | 267, 291 |
| BsaJI | CCNNGG | 3 cut(s) | 85, 314, 483 |
| BsaWI | WCCGGW | 1 cut(s) | 169 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 29, 358, 478, 484, 589, 592 |
| Bse1I | ACTGG | 2 cut(s) | 388, 567 |
| BseBI | CCWGG | 3 cut(s) | 315, 467, 479 |
| BseDI | CCNNGG | 3 cut(s) | 85, 314, 483 |
| BseGI | GGATG | 3 cut(s) | 58, 178, 474 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 29, 358, 478, 484, 589, 592 |
| BseMII | CTCAG | 1 cut(s) | 278 |
| BseNI | ACTGG | 2 cut(s) | 388, 567 |
| BseRI | GAGGAG | 2 cut(s) | 587, 608 |
| BseXI | GCAGC | 1 cut(s) | 615 |
| BshFI | GGCC | 1 cut(s) | 482 |
| BshNI | GGYRCC | 1 cut(s) | 664 |
| BsiSI | CCGG | 3 cut(s) | 170, 258, 612 |
| BslFI | GGGAC | 3 cut(s) | 206, 461, 480 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 29, 358, 478, 484, 589, 592 |
| BsmAI | GTCTC | 3 cut(s) | 208, 267, 291 |
| BsmFI | GGGAC | 3 cut(s) | 206, 461, 480 |
| BsnI | GGCC | 1 cut(s) | 482 |
| Bso31I | GGTCTC | 2 cut(s) | 267, 291 |
| Bsp143I | GATC | 3 cut(s) | 55, 105, 391 |
| BspACI | CCGC | 4 cut(s) | 282, 352, 661, 701 |
| BspANI | GGCC | 1 cut(s) | 482 |
| BspCNI | CTCAG | 1 cut(s) | 277 |
| BspLI | GGNNCC | 5 cut(s) | 194, 195, 256, 449, 666 |
| BspPI | GGATC | 2 cut(s) | 113, 399 |
| BspT107I | GGYRCC | 1 cut(s) | 664 |
| BspTNI | GGTCTC | 2 cut(s) | 267, 291 |
| BsrBI | CCGCTC | 2 cut(s) | 661, 701 |
| BsrI | ACTGG | 2 cut(s) | 388, 567 |
| BssECI | CCNNGG | 3 cut(s) | 85, 314, 483 |
| BssMI | GATC | 3 cut(s) | 55, 105, 391 |
| BssT1I | CCWWGG | 2 cut(s) | 85, 483 |
| Bst2UI | CCWGG | 3 cut(s) | 315, 467, 479 |
| Bst4CI | ACNGT | 4 cut(s) | 142, 347, 410, 494 |
| Bst6I | CTCTTC | 2 cut(s) | 33, 317 |
| BstC8I | GCNNGC | 2 cut(s) | 516, 537 |
| BstDEI | CTNAG | 2 cut(s) | 264, 443 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 590 |
| BstF5I | GGATG | 3 cut(s) | 58, 178, 474 |
| BstHHI | GCGC | 1 cut(s) | 541 |
| BstKTI | GATC | 3 cut(s) | 58, 108, 394 |
| BstMAI | GTCTC | 3 cut(s) | 208, 267, 291 |
| BstMBI | GATC | 3 cut(s) | 55, 105, 391 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 394, 563 |
| BstNI | CCWGG | 3 cut(s) | 315, 467, 479 |
| BstNSI | RCATGY | 1 cut(s) | 76 |
| BstSCI | CCNGG | 5 cut(s) | 257, 313, 465, 477, 611 |
| BstV1I | GCAGC | 1 cut(s) | 615 |
| BstX2I | RGATCY | 1 cut(s) | 391 |
| BstYI | RGATCY | 1 cut(s) | 391 |
| BsuI | GTATCC | 1 cut(s) | 571 |
| BsuRI | GGCC | 1 cut(s) | 482 |
| BtgZI | GCGATG | 1 cut(s) | 323 |
| BtrI | CACGTC | 1 cut(s) | 112 |
| BtsCI | GGATG | 3 cut(s) | 58, 178, 474 |
| BtsIMutI | CAGTG | 1 cut(s) | 574 |
| Cac8I | GCNNGC | 2 cut(s) | 516, 537 |
| CfoI | GCGC | 1 cut(s) | 541 |
| Cfr13I | GGNCC | 3 cut(s) | 193, 448, 469 |
| Csp6I | GTAC | 3 cut(s) | 406, 625, 665 |
| CviAII | CATG | 3 cut(s) | 73, 632, 672 |
| CviJI | RGCY | 9 cut(s) | 22, 224, 269, 388, 482, 514, 606, 649, 689 |
| CviKI_1 | RGCY | 9 cut(s) | 22, 224, 269, 388, 482, 514, 606, 649, 689 |
| CviQI | GTAC | 3 cut(s) | 406, 625, 665 |
| DdeI | CTNAG | 2 cut(s) | 264, 443 |
| DpnI | GATC | 3 cut(s) | 57, 107, 393 |
| DpnII | GATC | 3 cut(s) | 55, 105, 391 |
| Eam1104I | CTCTTC | 2 cut(s) | 33, 317 |
| EarI | CTCTTC | 2 cut(s) | 33, 317 |
| Eco130I | CCWWGG | 2 cut(s) | 85, 483 |
| Eco31I | GGTCTC | 2 cut(s) | 267, 291 |
| Eco32I | GATATC | 1 cut(s) | 154 |
| Eco47I | GGWCC | 3 cut(s) | 193, 448, 469 |
| Eco57I | CTGAAG | 1 cut(s) | 608 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 590 |
| EcoO109I | RGGNCCY | 2 cut(s) | 193, 448 |
| EcoRII | CCWGG | 3 cut(s) | 313, 465, 477 |
| EcoRV | GATATC | 1 cut(s) | 154 |
| EcoT14I | CCWWGG | 2 cut(s) | 85, 483 |
| ErhI | CCWWGG | 2 cut(s) | 85, 483 |
| FaeI | CATG | 3 cut(s) | 76, 635, 675 |
| FaiI | YATR | 8 cut(s) | 74, 183, 379, 381, 544, 555, 633, 673 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 141, 173 |
| FaqI | GGGAC | 3 cut(s) | 206, 461, 480 |
| FatI | CATG | 3 cut(s) | 72, 631, 671 |
| FauI | CCCGC | 3 cut(s) | 275, 654, 694 |
| Fnu4HI | GCNGC | 1 cut(s) | 604 |
| FokI | GGATG | 3 cut(s) | 65, 185, 461 |
| Fsp4HI | GCNGC | 1 cut(s) | 604 |
| GlaI | GCGC | 1 cut(s) | 540 |
| GluI | GCNGC | 1 cut(s) | 604 |
| GsuI | CTGGAG | 3 cut(s) | 182, 671, 711 |
| HaeIII | GGCC | 1 cut(s) | 482 |
| HapII | CCGG | 3 cut(s) | 170, 258, 612 |
| HhaI | GCGC | 1 cut(s) | 541 |
| Hin1II | CATG | 3 cut(s) | 76, 635, 675 |
| Hin6I | GCGC | 1 cut(s) | 539 |
| HinP1I | GCGC | 1 cut(s) | 539 |
| HinfI | GANTC | 2 cut(s) | 524, 705 |
| HpaII | CCGG | 3 cut(s) | 170, 258, 612 |
| HphI | GGTGA | 3 cut(s) | 341, 385, 610 |
| Hpy188I | TCNGA | 1 cut(s) | 403 |
| Hpy188III | TCNNGA | 2 cut(s) | 161, 228 |
| Hpy99I | CGWCG | 1 cut(s) | 113 |
| HpyAV | CCTTC | 8 cut(s) | 167, 583, 634, 638, 649, 674, 678, 689 |
| HpyCH4III | ACNGT | 4 cut(s) | 142, 347, 410, 494 |
| HpyCH4IV | ACGT | 3 cut(s) | 6, 111, 549 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 394, 563 |
| HpyF3I | CTNAG | 2 cut(s) | 264, 443 |
| HpySE526I | ACGT | 3 cut(s) | 6, 111, 549 |
| Hsp92II | CATG | 3 cut(s) | 76, 635, 675 |
| HspAI | GCGC | 1 cut(s) | 539 |
| KflI | GGGWCCC | 1 cut(s) | 193 |
| KpnI | GGTACC | 1 cut(s) | 668 |
| Kzo9I | GATC | 3 cut(s) | 55, 105, 391 |
| LmnI | GCTCC | 2 cut(s) | 658, 698 |
| Lsp1109I | GCAGC | 1 cut(s) | 615 |
| LweI | GCATC | 2 cut(s) | 429, 522 |
| MaeII | ACGT | 3 cut(s) | 6, 111, 549 |
| MaeIII | GTNAC | 3 cut(s) | 99, 347, 373 |
| MalI | GATC | 3 cut(s) | 57, 107, 393 |
| MbiI | CCGCTC | 2 cut(s) | 661, 701 |
| MboI | GATC | 3 cut(s) | 55, 105, 391 |
| MboII | GAAGA | 3 cut(s) | 50, 161, 334 |
| MflI | RGATCY | 1 cut(s) | 391 |
| MluCI | AATT | 2 cut(s) | 144, 425 |
| MlyI | GAGTC | 2 cut(s) | 533, 699 |
| MspA1I | CMGCKG | 1 cut(s) | 282 |
| MspI | CCGG | 3 cut(s) | 170, 258, 612 |
| MspR9I | CCNGG | 5 cut(s) | 259, 315, 467, 479, 613 |
| MvaI | CCWGG | 3 cut(s) | 315, 467, 479 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 394, 563 |
| NciI | CCSGG | 2 cut(s) | 259, 613 |
| NdeII | GATC | 3 cut(s) | 55, 105, 391 |
| NlaIII | CATG | 3 cut(s) | 76, 635, 675 |
| NlaIV | GGNNCC | 5 cut(s) | 194, 195, 256, 449, 666 |
| NmuCI | GTSAC | 2 cut(s) | 347, 373 |
| NspI | RCATGY | 1 cut(s) | 76 |
| PciI | ACATGT | 1 cut(s) | 72 |
| PkrI | GCNGC | 1 cut(s) | 605 |
| PleI | GAGTC | 2 cut(s) | 532, 699 |
| PpsI | GAGTC | 2 cut(s) | 532, 699 |
| PpuMI | RGGWCCY | 2 cut(s) | 193, 448 |
| PscI | ACATGT | 1 cut(s) | 72 |
| Psp5II | RGGWCCY | 2 cut(s) | 193, 448 |
| Psp6I | CCWGG | 3 cut(s) | 313, 465, 477 |
| PspGI | CCWGG | 3 cut(s) | 313, 465, 477 |
| PspN4I | GGNNCC | 5 cut(s) | 194, 195, 256, 449, 666 |
| PspPI | GGNCC | 3 cut(s) | 193, 448, 469 |
| PspPPI | RGGWCCY | 2 cut(s) | 193, 448 |
| PsuI | RGATCY | 1 cut(s) | 391 |
| RsaI | GTAC | 3 cut(s) | 407, 626, 666 |
| RsaNI | GTAC | 3 cut(s) | 406, 625, 665 |
| SatI | GCNGC | 1 cut(s) | 604 |
| Sau3AI | GATC | 3 cut(s) | 55, 105, 391 |
| Sau96I | GGNCC | 3 cut(s) | 193, 448, 469 |
| SchI | GAGTC | 2 cut(s) | 533, 699 |
| ScrFI | CCNGG | 5 cut(s) | 259, 315, 467, 479, 613 |
| SfaNI | GCATC | 2 cut(s) | 429, 522 |
| SinI | GGWCC | 3 cut(s) | 193, 448, 469 |
| SmlI | CTYRAG | 1 cut(s) | 452 |
| SmoI | CTYRAG | 1 cut(s) | 452 |
| Sse9I | AATT | 2 cut(s) | 144, 425 |
| SsiI | CCGC | 4 cut(s) | 282, 352, 661, 701 |
| StyD4I | CCNGG | 5 cut(s) | 257, 313, 465, 477, 611 |
| StyI | CCWWGG | 2 cut(s) | 85, 483 |
| TaaI | ACNGT | 4 cut(s) | 142, 347, 410, 494 |
| TaiI | ACGT | 3 cut(s) | 9, 114, 552 |
| TaqI | TCGA | 4 cut(s) | 108, 437, 462, 527 |
| TasI | AATT | 2 cut(s) | 144, 425 |
| TscAI | CASTG | 1 cut(s) | 574 |
| TseFI | GTSAC | 2 cut(s) | 347, 373 |
| TseI | GCWGC | 1 cut(s) | 603 |
| Tsp45I | GTSAC | 2 cut(s) | 347, 373 |
| TspDTI | ATGAA | 6 cut(s) | 17, 189, 326, 435, 620, 660 |
| TspGWI | ACGGA | 1 cut(s) | 118 |
| TspRI | CASTG | 1 cut(s) | 574 |
| VpaK11BI | GGWCC | 3 cut(s) | 193, 448, 469 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 590 |
| XapI | RAATTY | 1 cut(s) | 425 |
| XceI | RCATGY | 1 cut(s) | 76 |