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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 537 |
| AbsI | CCTCGAGG | 1 cut(s) | 269 |
| AccI | GTMKAC | 1 cut(s) | 149 |
| AccII | CGCG | 1 cut(s) | 366 |
| AciI | CCGC | 4 cut(s) | 364, 366, 427, 696 |
| AclWI | GGATC | 1 cut(s) | 500 |
| AfaI | GTAC | 2 cut(s) | 183, 446 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 45, 70, 172, 365, 369, 670 |
| AflIII | ACRYGT | 1 cut(s) | 16 |
| AgsI | TTSAA | 3 cut(s) | 35, 335, 559 |
| AjnI | CCWGG | 1 cut(s) | 250 |
| AluBI | AGCT | 4 cut(s) | 119, 214, 286, 574 |
| AluI | AGCT | 4 cut(s) | 119, 214, 286, 574 |
| AlwI | GGATC | 1 cut(s) | 500 |
| Ama87I | CYCGRG | 2 cut(s) | 81, 269 |
| AoxI | GGCC | 5 cut(s) | 47, 76, 85, 348, 614 |
| ApeKI | GCWGC | 1 cut(s) | 430 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 633, 665 |
| AspS9I | GGNCC | 6 cut(s) | 47, 61, 77, 85, 348, 372 |
| AsuC2I | CCSGG | 1 cut(s) | 502 |
| AsuHPI | GGTGA | 2 cut(s) | 643, 685 |
| AvaI | CYCGRG | 2 cut(s) | 81, 269 |
| AvaII | GGWCC | 2 cut(s) | 61, 372 |
| AxyI | CCTNAGG | 1 cut(s) | 488 |
| BauI | CACGAG | 2 cut(s) | 256, 667 |
| BbsI | GAAGAC | 1 cut(s) | 123 |
| BbvCI | CCTCAGC | 1 cut(s) | 611 |
| BbvI | GCAGC | 1 cut(s) | 417 |
| BccI | CCATC | 1 cut(s) | 224 |
| BceAI | ACGGC | 2 cut(s) | 34, 446 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 542, 576 |
| BciT130I | CCWGG | 1 cut(s) | 252 |
| BcnI | CCSGG | 1 cut(s) | 502 |
| BfaI | CTAG | 3 cut(s) | 125, 185, 222 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 84 |
| BglII | AGATCT | 1 cut(s) | 643 |
| BisI | GCNGC | 2 cut(s) | 428, 431 |
| BlsI | GCNGC | 2 cut(s) | 429, 432 |
| Bme1390I | CCNGG | 2 cut(s) | 252, 502 |
| Bme18I | GGWCC | 2 cut(s) | 61, 372 |
| BmeT110I | CYCGRG | 2 cut(s) | 81, 269 |
| BmgT120I | GGNCC | 6 cut(s) | 47, 61, 77, 85, 348, 372 |
| BmiI | GGNNCC | 2 cut(s) | 48, 86 |
| BmrFI | CCNGG | 2 cut(s) | 252, 502 |
| BpiI | GAAGAC | 1 cut(s) | 123 |
| BpmI | CTGGAG | 1 cut(s) | 47 |
| Bpu10I | CCTNAGC | 2 cut(s) | 287, 611 |
| BpuMI | CCSGG | 1 cut(s) | 502 |
| BsaJI | CCNNGG | 6 cut(s) | 64, 80, 96, 364, 501, 617 |
| BsaWI | WCCGGW | 1 cut(s) | 38 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 588, 618 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 45, 70, 172, 365, 369, 670 |
| Bse118I | RCCGGY | 1 cut(s) | 692 |
| Bse1I | ACTGG | 1 cut(s) | 351 |
| Bse21I | CCTNAGG | 1 cut(s) | 488 |
| BseBI | CCWGG | 1 cut(s) | 252 |
| BseDI | CCNNGG | 6 cut(s) | 64, 80, 96, 364, 501, 617 |
| BseGI | GGATG | 1 cut(s) | 355 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 45, 70, 172, 365, 369, 670 |
| BseMII | CTCAG | 4 cut(s) | 181, 333, 502, 602 |
| BseNI | ACTGG | 1 cut(s) | 351 |
| BseRI | GAGGAG | 2 cut(s) | 113, 287 |
| BseXI | GCAGC | 1 cut(s) | 417 |
| BseYI | CCCAGC | 1 cut(s) | 214 |
| Bsh1236I | CGCG | 1 cut(s) | 366 |
| BshFI | GGCC | 5 cut(s) | 49, 78, 87, 350, 616 |
| BsiHKCI | CYCGRG | 2 cut(s) | 81, 269 |
| BsiSI | CCGG | 3 cut(s) | 39, 501, 693 |
| BslFI | GGGAC | 1 cut(s) | 239 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 45, 70, 172, 365, 369, 670 |
| BsmFI | GGGAC | 1 cut(s) | 239 |
| BsnI | GGCC | 5 cut(s) | 49, 78, 87, 350, 616 |
| BsoBI | CYCGRG | 2 cut(s) | 81, 269 |
| Bsp143I | GATC | 3 cut(s) | 244, 492, 643 |
| BspACI | CCGC | 4 cut(s) | 364, 366, 427, 696 |
| BspANI | GGCC | 5 cut(s) | 49, 78, 87, 350, 616 |
| BspCNI | CTCAG | 4 cut(s) | 180, 334, 501, 603 |
| BspFNI | CGCG | 1 cut(s) | 366 |
| BspLI | GGNNCC | 2 cut(s) | 48, 86 |
| BspPI | GGATC | 1 cut(s) | 500 |
| BsrFI | RCCGGY | 1 cut(s) | 692 |
| BsrI | ACTGG | 1 cut(s) | 351 |
| BssAI | RCCGGY | 1 cut(s) | 692 |
| BssECI | CCNNGG | 6 cut(s) | 64, 80, 96, 364, 501, 617 |
| BssMI | GATC | 3 cut(s) | 244, 492, 643 |
| BssSI | CACGAG | 2 cut(s) | 256, 667 |
| BssT1I | CCWWGG | 1 cut(s) | 64 |
| Bst2BI | CACGAG | 2 cut(s) | 256, 667 |
| Bst2UI | CCWGG | 1 cut(s) | 252 |
| Bst4CI | ACNGT | 1 cut(s) | 316 |
| BstC8I | GCNNGC | 1 cut(s) | 694 |
| BstDEI | CTNAG | 6 cut(s) | 167, 287, 342, 395, 488, 611 |
| BstDSI | CCRYGG | 1 cut(s) | 364 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 170 |
| BstF5I | GGATG | 1 cut(s) | 355 |
| BstFNI | CGCG | 1 cut(s) | 366 |
| BstKTI | GATC | 3 cut(s) | 247, 495, 646 |
| BstMBI | GATC | 3 cut(s) | 244, 492, 643 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 75, 84, 693 |
| BstNI | CCWGG | 1 cut(s) | 252 |
| BstNSI | RCATGY | 1 cut(s) | 20 |
| BstSCI | CCNGG | 2 cut(s) | 250, 500 |
| BstUI | CGCG | 1 cut(s) | 366 |
| BstV1I | GCAGC | 1 cut(s) | 417 |
| BstV2I | GAAGAC | 1 cut(s) | 123 |
| BstX2I | RGATCY | 2 cut(s) | 492, 643 |
| BstYI | RGATCY | 2 cut(s) | 492, 643 |
| Bsu36I | CCTNAGG | 1 cut(s) | 488 |
| BsuRI | GGCC | 5 cut(s) | 49, 78, 87, 350, 616 |
| BtgI | CCRYGG | 1 cut(s) | 364 |
| BtsCI | GGATG | 1 cut(s) | 355 |
| Cac8I | GCNNGC | 1 cut(s) | 694 |
| Cfr10I | RCCGGY | 1 cut(s) | 692 |
| Cfr13I | GGNCC | 6 cut(s) | 47, 61, 77, 85, 348, 372 |
| Cfr42I | CCGCGG | 1 cut(s) | 367 |
| Csp6I | GTAC | 2 cut(s) | 182, 445 |
| CviAII | CATG | 3 cut(s) | 17, 89, 565 |
| CviQI | GTAC | 2 cut(s) | 182, 445 |
| DdeI | CTNAG | 6 cut(s) | 167, 287, 342, 395, 488, 611 |
| DpnI | GATC | 3 cut(s) | 246, 494, 645 |
| DpnII | GATC | 3 cut(s) | 244, 492, 643 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 537 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 537 |
| Eco130I | CCWWGG | 1 cut(s) | 64 |
| Eco147I | AGGCCT | 1 cut(s) | 616 |
| Eco47I | GGWCC | 2 cut(s) | 61, 372 |
| Eco81I | CCTNAGG | 1 cut(s) | 488 |
| Eco88I | CYCGRG | 2 cut(s) | 81, 269 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 170 |
| EcoRII | CCWGG | 1 cut(s) | 250 |
| EcoT14I | CCWWGG | 1 cut(s) | 64 |
| ErhI | CCWWGG | 1 cut(s) | 64 |
| FaeI | CATG | 3 cut(s) | 20, 92, 568 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 671, 703 |
| FaqI | GGGAC | 1 cut(s) | 239 |
| FatI | CATG | 3 cut(s) | 16, 88, 564 |
| FauI | CCCGC | 1 cut(s) | 371 |
| FblI | GTMKAC | 1 cut(s) | 149 |
| Fnu4HI | GCNGC | 2 cut(s) | 428, 431 |
| FokI | GGATG | 1 cut(s) | 342 |
| Fsp4HI | GCNGC | 2 cut(s) | 428, 431 |
| FspBI | CTAG | 3 cut(s) | 125, 185, 222 |
| GluI | GCNGC | 2 cut(s) | 428, 431 |
| GsaI | CCCAGC | 1 cut(s) | 218 |
| GsuI | CTGGAG | 1 cut(s) | 47 |
| HaeIII | GGCC | 5 cut(s) | 49, 78, 87, 350, 616 |
| HapII | CCGG | 3 cut(s) | 39, 501, 693 |
| Hin1II | CATG | 3 cut(s) | 20, 92, 568 |
| HindIII | AAGCTT | 2 cut(s) | 117, 572 |
| HinfI | GANTC | 3 cut(s) | 35, 128, 475 |
| HpaII | CCGG | 3 cut(s) | 39, 501, 693 |
| HphI | GGTGA | 2 cut(s) | 643, 685 |
| Hpy166II | GTNNAC | 2 cut(s) | 150, 530 |
| Hpy188I | TCNGA | 3 cut(s) | 55, 543, 621 |
| Hpy188III | TCNNGA | 3 cut(s) | 113, 308, 490 |
| Hpy8I | GTNNAC | 2 cut(s) | 150, 530 |
| HpyCH4III | ACNGT | 1 cut(s) | 316 |
| HpyCH4V | TGCA | 3 cut(s) | 325, 402, 433 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 75, 84, 693 |
| HpyF3I | CTNAG | 6 cut(s) | 167, 287, 342, 395, 488, 611 |
| Hsp92II | CATG | 3 cut(s) | 20, 92, 568 |
| KroI | GCCGGC | 1 cut(s) | 692 |
| KroNI | GCCGGC | 1 cut(s) | 694 |
| KspI | CCGCGG | 1 cut(s) | 367 |
| Kzo9I | GATC | 3 cut(s) | 244, 492, 643 |
| Lsp1109I | GCAGC | 1 cut(s) | 417 |
| MaeI | CTAG | 3 cut(s) | 125, 185, 222 |
| MaeIII | GTNAC | 2 cut(s) | 604, 649 |
| MalI | GATC | 3 cut(s) | 246, 494, 645 |
| MboI | GATC | 3 cut(s) | 244, 492, 643 |
| MboII | GAAGA | 4 cut(s) | 123, 347, 532, 653 |
| MflI | RGATCY | 2 cut(s) | 492, 643 |
| MluCI | AATT | 1 cut(s) | 295 |
| MlyI | GAGTC | 2 cut(s) | 137, 469 |
| MmeI | TCCRAC | 1 cut(s) | 350 |
| MreI | CGCCGGCG | 1 cut(s) | 692 |
| MroNI | GCCGGC | 1 cut(s) | 692 |
| MseI | TTAA | 3 cut(s) | 210, 279, 497 |
| MslI | CAYNNNNRTG | 1 cut(s) | 260 |
| MspA1I | CMGCKG | 1 cut(s) | 366 |
| MspI | CCGG | 3 cut(s) | 39, 501, 693 |
| MspR9I | CCNGG | 2 cut(s) | 252, 502 |
| MvaI | CCWGG | 1 cut(s) | 252 |
| MvnI | CGCG | 1 cut(s) | 366 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 75, 84, 693 |
| NaeI | GCCGGC | 1 cut(s) | 694 |
| NciI | CCSGG | 1 cut(s) | 502 |
| NdeII | GATC | 3 cut(s) | 244, 492, 643 |
| NgoMIV | GCCGGC | 1 cut(s) | 692 |
| NlaIII | CATG | 3 cut(s) | 20, 92, 568 |
| NlaIV | GGNNCC | 2 cut(s) | 48, 86 |
| NmeAIII | GCCGAG | 3 cut(s) | 121, 403, 563 |
| NmuCI | GTSAC | 1 cut(s) | 649 |
| NspI | RCATGY | 1 cut(s) | 20 |
| PaeR7I | CTCGAG | 1 cut(s) | 269 |
| PceI | AGGCCT | 1 cut(s) | 616 |
| PciI | ACATGT | 1 cut(s) | 16 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 645 |
| PdiI | GCCGGC | 1 cut(s) | 694 |
| PfeI | GAWTC | 1 cut(s) | 35 |
| PkrI | GCNGC | 2 cut(s) | 429, 432 |
| PleI | GAGTC | 2 cut(s) | 136, 469 |
| PpsI | GAGTC | 2 cut(s) | 136, 469 |
| PscI | ACATGT | 1 cut(s) | 16 |
| Psp6I | CCWGG | 1 cut(s) | 250 |
| PspFI | CCCAGC | 1 cut(s) | 214 |
| PspGI | CCWGG | 1 cut(s) | 250 |
| PspN4I | GGNNCC | 2 cut(s) | 48, 86 |
| PspPI | GGNCC | 6 cut(s) | 47, 61, 77, 85, 348, 372 |
| PspXI | VCTCGAGB | 1 cut(s) | 269 |
| PsuI | RGATCY | 2 cut(s) | 492, 643 |
| RsaI | GTAC | 2 cut(s) | 183, 446 |
| RsaNI | GTAC | 2 cut(s) | 182, 445 |
| RseI | CAYNNNNRTG | 1 cut(s) | 260 |
| SacII | CCGCGG | 1 cut(s) | 367 |
| SaqAI | TTAA | 3 cut(s) | 210, 279, 497 |
| SatI | GCNGC | 2 cut(s) | 428, 431 |
| Sau3AI | GATC | 3 cut(s) | 244, 492, 643 |
| Sau96I | GGNCC | 6 cut(s) | 47, 61, 77, 85, 348, 372 |
| SchI | GAGTC | 2 cut(s) | 137, 469 |
| ScrFI | CCNGG | 2 cut(s) | 252, 502 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 84 |
| Sfr274I | CTCGAG | 1 cut(s) | 269 |
| Sfr303I | CCGCGG | 1 cut(s) | 367 |
| SgrAI | CRCCGGYG | 1 cut(s) | 692 |
| SgrBI | CCGCGG | 1 cut(s) | 367 |
| SinI | GGWCC | 2 cut(s) | 61, 372 |
| SlaI | CTCGAG | 1 cut(s) | 269 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 260 |
| SmlI | CTYRAG | 1 cut(s) | 269 |
| SmoI | CTYRAG | 1 cut(s) | 269 |
| Sse9I | AATT | 1 cut(s) | 295 |
| SseBI | AGGCCT | 1 cut(s) | 616 |
| SsiI | CCGC | 4 cut(s) | 364, 366, 427, 696 |
| SspI | AATATT | 1 cut(s) | 658 |
| SspMI | CTAG | 3 cut(s) | 125, 185, 222 |
| StuI | AGGCCT | 1 cut(s) | 616 |
| StyD4I | CCNGG | 2 cut(s) | 250, 500 |
| StyI | CCWWGG | 1 cut(s) | 64 |
| TaaI | ACNGT | 1 cut(s) | 316 |
| TaqI | TCGA | 4 cut(s) | 141, 270, 309, 639 |
| TasI | AATT | 1 cut(s) | 295 |
| TatI | WGTACW | 1 cut(s) | 444 |
| TauI | GCSGC | 1 cut(s) | 430 |
| TfiI | GAWTC | 1 cut(s) | 35 |
| Tru1I | TTAA | 3 cut(s) | 210, 279, 497 |
| Tru9I | TTAA | 3 cut(s) | 210, 279, 497 |
| TseFI | GTSAC | 1 cut(s) | 649 |
| TseI | GCWGC | 1 cut(s) | 430 |
| Tsp45I | GTSAC | 1 cut(s) | 649 |
| TspDTI | ATGAA | 3 cut(s) | 26, 105, 567 |
| TspGWI | ACGGA | 1 cut(s) | 74 |
| VpaK11BI | GGWCC | 2 cut(s) | 61, 372 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 170 |
| XceI | RCATGY | 1 cut(s) | 20 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 71 |
| XhoI | CTCGAG | 1 cut(s) | 269 |
| XmiI | GTMKAC | 1 cut(s) | 149 |
| XspI | CTAG | 3 cut(s) | 125, 185, 222 |