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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1142 |
| AarI | CACCTGC | 1 cut(s) | 1513 |
| Acc36I | ACCTGC | 1 cut(s) | 1513 |
| Acc65I | GGTACC | 1 cut(s) | 740 |
| AccB1I | GGYRCC | 4 cut(s) | 423, 740, 1183, 1934 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 964 |
| AccBSI | CCGCTC | 1 cut(s) | 1567 |
| AccI | GTMKAC | 2 cut(s) | 810, 1011 |
| AccII | CGCG | 8 cut(s) | 161, 311, 558, 560, 1100, 1118, 1742, 1919 |
| AclWI | GGATC | 7 cut(s) | 13, 150, 163, 1362, 1616, 1917, 1963 |
| AcoI | YGGCCR | 4 cut(s) | 183, 345, 1131, 1590 |
| AcsI | RAATTY | 1 cut(s) | 1874 |
| AcuI | CTGAAG | 3 cut(s) | 311, 1379, 1514 |
| AcyI | GRCGYC | 1 cut(s) | 993 |
| AgeI | ACCGGT | 2 cut(s) | 51, 737 |
| AgsI | TTSAA | 3 cut(s) | 1046, 1453, 2050 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1018 |
| AhlI | ACTAGT | 1 cut(s) | 637 |
| AjiI | CACGTC | 1 cut(s) | 1114 |
| AjnI | CCWGG | 5 cut(s) | 26, 981, 1179, 1404, 1722 |
| Alw21I | GWGCWC | 2 cut(s) | 842, 1788 |
| Alw26I | GTCTC | 2 cut(s) | 1702, 1950 |
| AlwI | GGATC | 7 cut(s) | 13, 150, 163, 1362, 1616, 1917, 1963 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 436, 1303, 1691 |
| Ama87I | CYCGRG | 7 cut(s) | 63, 191, 248, 361, 1425, 1641, 1662 |
| ApoI | RAATTY | 1 cut(s) | 1874 |
| AsiGI | ACCGGT | 2 cut(s) | 51, 737 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 389, 1053 |
| Asp718I | GGTACC | 1 cut(s) | 740 |
| AspA2I | CCTAGG | 1 cut(s) | 1015 |
| AspLEI | GCGC | 5 cut(s) | 376, 560, 907, 1102, 1120 |
| AsuHPI | GGTGA | 4 cut(s) | 152, 1094, 1230, 1537 |
| AvaI | CYCGRG | 7 cut(s) | 63, 191, 248, 361, 1425, 1641, 1662 |
| AvaII | GGWCC | 4 cut(s) | 231, 760, 1341, 2058 |
| AvrII | CCTAGG | 1 cut(s) | 1015 |
| BaeGI | GKGCMC | 1 cut(s) | 1447 |
| BalI | TGGCCA | 1 cut(s) | 185 |
| BamHI | GGATCC | 1 cut(s) | 155 |
| BanI | GGYRCC | 4 cut(s) | 423, 740, 1183, 1934 |
| BanII | GRGCYC | 2 cut(s) | 842, 1788 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 197, 229 |
| BauI | CACGAG | 2 cut(s) | 380, 1751 |
| BbsI | GAAGAC | 5 cut(s) | 12, 87, 996, 1880, 1991 |
| Bbv12I | GWGCWC | 2 cut(s) | 842, 1788 |
| BbvCI | CCTCAGC | 2 cut(s) | 36, 209 |
| BccI | CCATC | 8 cut(s) | 329, 337, 499, 958, 970, 1156, 1298, 2069 |
| BceAI | ACGGC | 4 cut(s) | 800, 1679, 1742, 2017 |
| BciT130I | CCWGG | 5 cut(s) | 28, 983, 1181, 1406, 1724 |
| BciVI | GTATCC | 1 cut(s) | 1477 |
| BcoDI | GTCTC | 2 cut(s) | 1702, 1950 |
| BcuI | ACTAGT | 1 cut(s) | 637 |
| BfaI | CTAG | 8 cut(s) | 621, 638, 692, 878, 884, 1016, 2022, 2028 |
| BfmI | CTRYAG | 3 cut(s) | 428, 1683, 1993 |
| BfoI | RGCGCY | 2 cut(s) | 377, 908 |
| BfuAI | ACCTGC | 1 cut(s) | 1513 |
| BfuI | GTATCC | 1 cut(s) | 1477 |
| BlnI | CCTAGG | 1 cut(s) | 1015 |
| BlpI | GCTNAGC | 1 cut(s) | 1599 |
| Bme18I | GGWCC | 4 cut(s) | 231, 760, 1341, 2058 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1018 |
| BmeT110I | CYCGRG | 7 cut(s) | 63, 191, 248, 361, 1425, 1641, 1662 |
| BmgBI | CACGTC | 1 cut(s) | 1114 |
| BmrI | ACTGGG | 3 cut(s) | 111, 470, 1215 |
| BmsI | GCATC | 4 cut(s) | 435, 943, 1428, 1459 |
| BmuI | ACTGGG | 3 cut(s) | 111, 470, 1215 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1112 |
| BpiI | GAAGAC | 5 cut(s) | 12, 87, 996, 1880, 1991 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 824, 856, 1775, 1807 |
| BpmI | CTGGAG | 4 cut(s) | 1079, 1577, 1745, 1913 |
| Bpu10I | CCTNAGC | 3 cut(s) | 36, 209, 1988 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1599 |
| BpuEI | CTTGAG | 2 cut(s) | 565, 1640 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1488 |
| BsaHI | GRCGYC | 1 cut(s) | 993 |
| BsaI | GGTCTC | 1 cut(s) | 1950 |
| BsaWI | WCCGGW | 3 cut(s) | 51, 737, 1853 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1757, 1787 |
| Bse118I | RCCGGY | 4 cut(s) | 51, 737, 1174, 1937 |
| Bse1I | ACTGG | 5 cut(s) | 16, 106, 465, 763, 1210 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1488 |
| BseBI | CCWGG | 5 cut(s) | 28, 983, 1181, 1406, 1724 |
| BseGI | GGATG | 6 cut(s) | 340, 450, 852, 955, 1167, 2061 |
| BseJI | GATNNNNATC | 1 cut(s) | 1488 |
| BseMII | CTCAG | 7 cut(s) | 27, 200, 1221, 1295, 1626, 1662, 1961 |
| BseNI | ACTGG | 5 cut(s) | 16, 106, 465, 763, 1210 |
| BseRI | GAGGAG | 7 cut(s) | 140, 850, 950, 1183, 1493, 1514, 1778 |
| BseSI | GKGCMC | 1 cut(s) | 1447 |
| BseYI | CCCAGC | 1 cut(s) | 695 |
| BsgI | GTGCAG | 2 cut(s) | 362, 1791 |
| Bsh1236I | CGCG | 8 cut(s) | 161, 311, 558, 560, 1100, 1118, 1742, 1919 |
| Bsh1285I | CGRYCG | 3 cut(s) | 52, 1378, 1854 |
| BshNI | GGYRCC | 4 cut(s) | 423, 740, 1183, 1934 |
| BshTI | ACCGGT | 2 cut(s) | 51, 737 |
| BsiEI | CGRYCG | 3 cut(s) | 52, 1378, 1854 |
| BsiHKAI | GWGCWC | 2 cut(s) | 842, 1788 |
| BsiHKCI | CYCGRG | 7 cut(s) | 63, 191, 248, 361, 1425, 1641, 1662 |
| BslFI | GGGAC | 7 cut(s) | 244, 300, 530, 773, 1583, 1679, 1928 |
| BsmAI | GTCTC | 2 cut(s) | 1702, 1950 |
| BsmBI | CGTCTC | 1 cut(s) | 1702 |
| BsmFI | GGGAC | 7 cut(s) | 244, 300, 530, 773, 1583, 1679, 1928 |
| BsmI | GAATGC | 2 cut(s) | 198, 484 |
| Bso31I | GGTCTC | 1 cut(s) | 1950 |
| BsoBI | CYCGRG | 7 cut(s) | 63, 191, 248, 361, 1425, 1641, 1662 |
| Bsp1286I | GDGCHC | 3 cut(s) | 842, 1447, 1788 |
| Bsp143I | GATC | 9 cut(s) | 5, 70, 155, 273, 1062, 1354, 1608, 1909, 1968 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1599 |
| Bsp19I | CCATGG | 1 cut(s) | 1128 |
| BspCNI | CTCAG | 7 cut(s) | 28, 201, 1222, 1296, 1627, 1663, 1960 |
| BspFNI | CGCG | 8 cut(s) | 161, 311, 558, 560, 1100, 1118, 1742, 1919 |
| BspHI | TCATGA | 2 cut(s) | 1827, 1965 |
| BspMAI | CTGCAG | 1 cut(s) | 1687 |
| BspMI | ACCTGC | 1 cut(s) | 1513 |
| BspPI | GGATC | 7 cut(s) | 13, 150, 163, 1362, 1616, 1917, 1963 |
| BspQI | GCTCTTC | 1 cut(s) | 1776 |
| BspT107I | GGYRCC | 4 cut(s) | 423, 740, 1183, 1934 |
| BspTNI | GGTCTC | 1 cut(s) | 1950 |
| BsrBI | CCGCTC | 1 cut(s) | 1567 |
| BsrFI | RCCGGY | 4 cut(s) | 51, 737, 1174, 1937 |
| BsrI | ACTGG | 5 cut(s) | 16, 106, 465, 763, 1210 |
| BssAI | RCCGGY | 4 cut(s) | 51, 737, 1174, 1937 |
| BssMI | GATC | 9 cut(s) | 5, 70, 155, 273, 1062, 1354, 1608, 1909, 1968 |
| BssNAI | GTATAC | 1 cut(s) | 811 |
| BssNI | GRCGYC | 1 cut(s) | 993 |
| BssSI | CACGAG | 2 cut(s) | 380, 1751 |
| BssT1I | CCWWGG | 4 cut(s) | 397, 1015, 1128, 1389 |
| Bst1107I | GTATAC | 1 cut(s) | 811 |
| Bst2BI | CACGAG | 2 cut(s) | 380, 1751 |
| Bst2UI | CCWGG | 5 cut(s) | 28, 983, 1181, 1406, 1724 |
| Bst4CI | ACNGT | 7 cut(s) | 181, 235, 414, 432, 500, 576, 607 |
| Bst6I | CTCTTC | 5 cut(s) | 788, 905, 1454, 1641, 1776 |
| BstACI | GRCGYC | 1 cut(s) | 993 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 356, 1691 |
| BstC8I | GCNNGC | 7 cut(s) | 33, 1443, 1474, 1590, 1736, 1740, 1800 |
| BstDSI | CCRYGG | 4 cut(s) | 159, 1128, 1740, 1758 |
| BstEII | GGTNACC | 1 cut(s) | 607 |
| BstF5I | GGATG | 6 cut(s) | 340, 450, 852, 955, 1167, 2061 |
| BstFNI | CGCG | 8 cut(s) | 161, 311, 558, 560, 1100, 1118, 1742, 1919 |
| BstH2I | RGCGCY | 2 cut(s) | 377, 908 |
| BstHHI | GCGC | 5 cut(s) | 376, 560, 907, 1102, 1120 |
| BstKTI | GATC | 9 cut(s) | 8, 73, 158, 276, 1065, 1357, 1611, 1912, 1971 |
| BstMAI | GTCTC | 2 cut(s) | 1702, 1950 |
| BstMBI | GATC | 9 cut(s) | 5, 70, 155, 273, 1062, 1354, 1608, 1909, 1968 |
| BstMCI | CGRYCG | 3 cut(s) | 52, 1378, 1854 |
| BstNI | CCWGG | 5 cut(s) | 28, 983, 1181, 1406, 1724 |
| BstNSI | RCATGY | 1 cut(s) | 652 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1112 |
| BstPI | GGTNACC | 1 cut(s) | 607 |
| BstSFI | CTRYAG | 3 cut(s) | 428, 1683, 1993 |
| BstSLI | GKGCMC | 1 cut(s) | 1447 |
| BstUI | CGCG | 8 cut(s) | 161, 311, 558, 560, 1100, 1118, 1742, 1919 |
| BstV2I | GAAGAC | 5 cut(s) | 12, 87, 996, 1880, 1991 |
| BstX2I | RGATCY | 2 cut(s) | 155, 1354 |
| BstYI | RGATCY | 2 cut(s) | 155, 1354 |
| BstZ17I | GTATAC | 1 cut(s) | 811 |
| BsuI | GTATCC | 1 cut(s) | 1477 |
| BtgI | CCRYGG | 4 cut(s) | 159, 1128, 1740, 1758 |
| BtgZI | GCGATG | 1 cut(s) | 1227 |
| BtrI | CACGTC | 1 cut(s) | 1114 |
| BtsCI | GGATG | 6 cut(s) | 340, 450, 852, 955, 1167, 2061 |
| BtsIMutI | CAGTG | 3 cut(s) | 186, 458, 1203 |
| BveI | ACCTGC | 1 cut(s) | 1513 |
| Cac8I | GCNNGC | 7 cut(s) | 33, 1443, 1474, 1590, 1736, 1740, 1800 |
| CaiI | CAGNNNCTG | 3 cut(s) | 436, 1303, 1691 |
| CciI | TCATGA | 2 cut(s) | 1827, 1965 |
| CfoI | GCGC | 5 cut(s) | 376, 560, 907, 1102, 1120 |
| Cfr10I | RCCGGY | 4 cut(s) | 51, 737, 1174, 1937 |
| Cfr42I | CCGCGG | 2 cut(s) | 162, 1743 |
| Cfr9I | CCCGGG | 6 cut(s) | 63, 248, 361, 1425, 1641, 1662 |
| CseI | GACGC | 5 cut(s) | 317, 1001, 1106, 1580, 1925 |
| CspAI | ACCGGT | 2 cut(s) | 51, 737 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 515, 550 |
| DpnI | GATC | 9 cut(s) | 7, 72, 157, 275, 1064, 1356, 1610, 1911, 1970 |
| DpnII | GATC | 9 cut(s) | 5, 70, 155, 273, 1062, 1354, 1608, 1909, 1968 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1018 |
| EaeI | YGGCCR | 4 cut(s) | 183, 345, 1131, 1590 |
| Eam1104I | CTCTTC | 5 cut(s) | 788, 905, 1454, 1641, 1776 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1018 |
| EarI | CTCTTC | 5 cut(s) | 788, 905, 1454, 1641, 1776 |
| EciI | GGCGGA | 1 cut(s) | 1598 |
| Ecl136II | GAGCTC | 2 cut(s) | 840, 1786 |
| Eco130I | CCWWGG | 4 cut(s) | 397, 1015, 1128, 1389 |
| Eco147I | AGGCCT | 1 cut(s) | 325 |
| Eco24I | GRGCYC | 2 cut(s) | 842, 1788 |
| Eco31I | GGTCTC | 1 cut(s) | 1950 |
| Eco32I | GATATC | 3 cut(s) | 46, 303, 1366 |
| Eco47I | GGWCC | 4 cut(s) | 231, 760, 1341, 2058 |
| Eco53kI | GAGCTC | 2 cut(s) | 840, 1786 |
| Eco57I | CTGAAG | 3 cut(s) | 311, 1379, 1514 |
| Eco88I | CYCGRG | 7 cut(s) | 63, 191, 248, 361, 1425, 1641, 1662 |
| Eco91I | GGTNACC | 1 cut(s) | 607 |
| EcoICRI | GAGCTC | 2 cut(s) | 840, 1786 |
| EcoO109I | RGGNCCY | 1 cut(s) | 166 |
| EcoO65I | GGTNACC | 1 cut(s) | 607 |
| EcoRII | CCWGG | 5 cut(s) | 26, 981, 1179, 1404, 1722 |
| EcoRV | GATATC | 3 cut(s) | 46, 303, 1366 |
| EcoT14I | CCWWGG | 4 cut(s) | 397, 1015, 1128, 1389 |
| EcoT38I | GRGCYC | 2 cut(s) | 842, 1788 |
| ErhI | CCWWGG | 4 cut(s) | 397, 1015, 1128, 1389 |
| Esp3I | CGTCTC | 1 cut(s) | 1702 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1038, 1070 |
| FaqI | GGGAC | 7 cut(s) | 244, 300, 530, 773, 1583, 1679, 1928 |
| FauI | CCCGC | 6 cut(s) | 592, 1270, 1434, 1560, 1747, 1875 |
| FblI | GTMKAC | 2 cut(s) | 810, 1011 |
| FokI | GGATG | 6 cut(s) | 347, 457, 839, 962, 1174, 2048 |
| FriOI | GRGCYC | 2 cut(s) | 842, 1788 |
| FspBI | CTAG | 8 cut(s) | 621, 638, 692, 878, 884, 1016, 2022, 2028 |
| GlaI | GCGC | 5 cut(s) | 375, 559, 906, 1101, 1119 |
| GsaI | CCCAGC | 1 cut(s) | 699 |
| GsuI | CTGGAG | 4 cut(s) | 1079, 1577, 1745, 1913 |
| HaeII | RGCGCY | 2 cut(s) | 377, 908 |
| HgaI | GACGC | 5 cut(s) | 317, 1001, 1106, 1580, 1925 |
| HhaI | GCGC | 5 cut(s) | 376, 560, 907, 1102, 1120 |
| Hin1I | GRCGYC | 1 cut(s) | 993 |
| Hin6I | GCGC | 5 cut(s) | 374, 558, 905, 1100, 1118 |
| HinP1I | GCGC | 5 cut(s) | 374, 558, 905, 1100, 1118 |
| HincII | GTYRAC | 2 cut(s) | 1012, 2037 |
| HindII | GTYRAC | 2 cut(s) | 1012, 2037 |
| HphI | GGTGA | 4 cut(s) | 152, 1094, 1230, 1537 |
| Hpy166II | GTNNAC | 7 cut(s) | 216, 547, 811, 1012, 1085, 1459, 2037 |
| Hpy188III | TCNNGA | 6 cut(s) | 191, 1058, 1828, 1892, 1913, 1966 |
| Hpy8I | GTNNAC | 7 cut(s) | 216, 547, 811, 1012, 1085, 1459, 2037 |
| Hpy99I | CGWCG | 6 cut(s) | 13, 137, 245, 1073, 1100, 1118 |
| HpyCH4III | ACNGT | 7 cut(s) | 181, 235, 414, 432, 500, 576, 607 |
| HpyCH4IV | ACGT | 1 cut(s) | 1113 |
| HpyCH4V | TGCA | 3 cut(s) | 379, 1685, 1808 |
| HpySE526I | ACGT | 1 cut(s) | 1113 |
| Hsp92I | GRCGYC | 1 cut(s) | 993 |
| HspAI | GCGC | 5 cut(s) | 374, 558, 905, 1100, 1118 |
| KpnI | GGTACC | 1 cut(s) | 744 |
| KspI | CCGCGG | 2 cut(s) | 162, 1743 |
| Kzo9I | GATC | 9 cut(s) | 5, 70, 155, 273, 1062, 1354, 1608, 1909, 1968 |
| LguI | GCTCTTC | 1 cut(s) | 1776 |
| LmnI | GCTCC | 4 cut(s) | 837, 897, 1209, 1585 |
| LweI | GCATC | 4 cut(s) | 435, 943, 1428, 1459 |
| MaeI | CTAG | 8 cut(s) | 621, 638, 692, 878, 884, 1016, 2022, 2028 |
| MaeII | ACGT | 1 cut(s) | 1113 |
| MaeIII | GTNAC | 6 cut(s) | 175, 456, 607, 640, 1105, 1236 |
| MalI | GATC | 9 cut(s) | 7, 72, 157, 275, 1064, 1356, 1610, 1911, 1970 |
| MbiI | CCGCTC | 1 cut(s) | 1567 |
| MboI | GATC | 9 cut(s) | 5, 70, 155, 273, 1062, 1354, 1608, 1909, 1968 |
| MfeI | CAATTG | 1 cut(s) | 1041 |
| MflI | RGATCY | 2 cut(s) | 155, 1354 |
| MhlI | GDGCHC | 3 cut(s) | 842, 1447, 1788 |
| MlsI | TGGCCA | 1 cut(s) | 185 |
| MluCI | AATT | 5 cut(s) | 74, 389, 1041, 1874, 2052 |
| MluNI | TGGCCA | 1 cut(s) | 185 |
| MlyI | GAGTC | 5 cut(s) | 137, 1042, 1241, 1661, 1697 |
| MmeI | TCCRAC | 1 cut(s) | 156 |
| Mox20I | TGGCCA | 1 cut(s) | 185 |
| MroXI | GAANNNNTTC | 2 cut(s) | 389, 1053 |
| MscI | TGGCCA | 1 cut(s) | 185 |
| MseI | TTAA | 1 cut(s) | 714 |
| MslI | CAYNNNNRTG | 3 cut(s) | 281, 1160, 1793 |
| Msp20I | TGGCCA | 1 cut(s) | 185 |
| MspA1I | CMGCKG | 6 cut(s) | 161, 469, 733, 1300, 1606, 1742 |
| MunI | CAATTG | 1 cut(s) | 1041 |
| Mva1269I | GAATGC | 2 cut(s) | 198, 484 |
| MvaI | CCWGG | 5 cut(s) | 28, 983, 1181, 1406, 1724 |
| MvnI | CGCG | 8 cut(s) | 161, 311, 558, 560, 1100, 1118, 1742, 1919 |
| NcoI | CCATGG | 1 cut(s) | 1128 |
| NdeII | GATC | 9 cut(s) | 5, 70, 155, 273, 1062, 1354, 1608, 1909, 1968 |
| NmuCI | GTSAC | 4 cut(s) | 175, 456, 1105, 1236 |
| NspI | RCATGY | 1 cut(s) | 652 |
| PaeR7I | CTCGAG | 1 cut(s) | 191 |
| PagI | TCATGA | 2 cut(s) | 1827, 1965 |
| PaqCI | CACCTGC | 1 cut(s) | 1513 |
| PceI | AGGCCT | 1 cut(s) | 325 |
| PciSI | GCTCTTC | 1 cut(s) | 1776 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 95, 1028, 1320 |
| PctI | GAATGC | 2 cut(s) | 198, 484 |
| PdmI | GAANNNNTTC | 2 cut(s) | 389, 1053 |
| PfeI | GAWTC | 5 cut(s) | 385, 849, 1275, 1411, 1430 |
| PflFI | GACNNNGTC | 1 cut(s) | 1927 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 964 |
| PfoI | TCCNGGA | 1 cut(s) | 1270 |
| PinAI | ACCGGT | 2 cut(s) | 51, 737 |
| PleI | GAGTC | 5 cut(s) | 136, 1041, 1240, 1661, 1696 |
| PpsI | GAGTC | 5 cut(s) | 136, 1041, 1240, 1661, 1696 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1112 |
| PsiI | TTATAA | 1 cut(s) | 1142 |
| Psp124BI | GAGCTC | 2 cut(s) | 842, 1788 |
| Psp6I | CCWGG | 5 cut(s) | 26, 981, 1179, 1404, 1722 |
| PspEI | GGTNACC | 1 cut(s) | 607 |
| PspFI | CCCAGC | 1 cut(s) | 695 |
| PspGI | CCWGG | 5 cut(s) | 26, 981, 1179, 1404, 1722 |
| PstI | CTGCAG | 1 cut(s) | 1687 |
| PstNI | CAGNNNCTG | 3 cut(s) | 436, 1303, 1691 |
| PsuI | RGATCY | 2 cut(s) | 155, 1354 |
| PsyI | GACNNNGTC | 1 cut(s) | 1927 |
| PvuII | CAGCTG | 3 cut(s) | 469, 733, 1300 |
| RseI | CAYNNNNRTG | 3 cut(s) | 281, 1160, 1793 |
| SacI | GAGCTC | 2 cut(s) | 842, 1788 |
| SacII | CCGCGG | 2 cut(s) | 162, 1743 |
| SalI | GTCGAC | 1 cut(s) | 1010 |
| SapI | GCTCTTC | 1 cut(s) | 1776 |
| SaqAI | TTAA | 1 cut(s) | 714 |
| Sau3AI | GATC | 9 cut(s) | 5, 70, 155, 273, 1062, 1354, 1608, 1909, 1968 |
| SchI | GAGTC | 5 cut(s) | 137, 1042, 1241, 1661, 1697 |
| SduI | GDGCHC | 3 cut(s) | 842, 1447, 1788 |
| SfaNI | GCATC | 4 cut(s) | 435, 943, 1428, 1459 |
| SfcI | CTRYAG | 3 cut(s) | 428, 1683, 1993 |
| Sfr274I | CTCGAG | 1 cut(s) | 191 |
| Sfr303I | CCGCGG | 2 cut(s) | 162, 1743 |
| SgrBI | CCGCGG | 2 cut(s) | 162, 1743 |
| SinI | GGWCC | 4 cut(s) | 231, 760, 1341, 2058 |
| SlaI | CTCGAG | 1 cut(s) | 191 |
| SmaI | CCCGGG | 6 cut(s) | 65, 250, 363, 1427, 1643, 1664 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 281, 1160, 1793 |
| SmlI | CTYRAG | 3 cut(s) | 191, 580, 1619 |
| SmoI | CTYRAG | 3 cut(s) | 191, 580, 1619 |
| SpeI | ACTAGT | 1 cut(s) | 637 |
| Sse9I | AATT | 5 cut(s) | 74, 389, 1041, 1874, 2052 |
| SseBI | AGGCCT | 1 cut(s) | 325 |
| SspMI | CTAG | 8 cut(s) | 621, 638, 692, 878, 884, 1016, 2022, 2028 |
| SstI | GAGCTC | 2 cut(s) | 842, 1788 |
| StuI | AGGCCT | 1 cut(s) | 325 |
| StyI | CCWWGG | 4 cut(s) | 397, 1015, 1128, 1389 |
| TaaI | ACNGT | 7 cut(s) | 181, 235, 414, 432, 500, 576, 607 |
| TaiI | ACGT | 1 cut(s) | 1116 |
| TaqII | GACCGA | 1 cut(s) | 1026 |
| TasI | AATT | 5 cut(s) | 74, 389, 1041, 1874, 2052 |
| TatI | WGTACW | 2 cut(s) | 576, 1767 |
| TauI | GCSGC | 5 cut(s) | 350, 620, 1595, 1598, 1922 |
| TfiI | GAWTC | 5 cut(s) | 385, 849, 1275, 1411, 1430 |
| Tru1I | TTAA | 1 cut(s) | 714 |
| Tru9I | TTAA | 1 cut(s) | 714 |
| TscAI | CASTG | 3 cut(s) | 186, 465, 1210 |
| TseFI | GTSAC | 4 cut(s) | 175, 456, 1105, 1236 |
| Tsp45I | GTSAC | 4 cut(s) | 175, 456, 1105, 1236 |
| TspDTI | ATGAA | 7 cut(s) | 281, 841, 1341, 1526, 1844, 1881, 1954 |
| TspGWI | ACGGA | 1 cut(s) | 532 |
| TspMI | CCCGGG | 6 cut(s) | 63, 248, 361, 1425, 1641, 1662 |
| TspRI | CASTG | 3 cut(s) | 186, 465, 1210 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1927 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 964 |
| VpaK11BI | GGWCC | 4 cut(s) | 231, 760, 1341, 2058 |
| XapI | RAATTY | 1 cut(s) | 1874 |
| XceI | RCATGY | 1 cut(s) | 652 |
| XhoI | CTCGAG | 1 cut(s) | 191 |
| XmaI | CCCGGG | 6 cut(s) | 63, 248, 361, 1425, 1641, 1662 |
| XmaJI | CCTAGG | 1 cut(s) | 1015 |
| XmiI | GTMKAC | 2 cut(s) | 810, 1011 |
| XmnI | GAANNNNTTC | 2 cut(s) | 389, 1053 |
| XspI | CTAG | 8 cut(s) | 621, 638, 692, 878, 884, 1016, 2022, 2028 |