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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 3 cut(s) | 188, 338, 377 |
| AciI | CCGC | 7 cut(s) | 107, 186, 188, 324, 338, 353, 375 |
| AclWI | GGATC | 3 cut(s) | 40, 177, 190 |
| AcoI | YGGCCR | 1 cut(s) | 372 |
| AcuI | CTGAAG | 2 cut(s) | 84, 338 |
| AfaI | GTAC | 3 cut(s) | 41, 83, 242 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 21, 129, 389, 393, 394 |
| AgeI | ACCGGT | 1 cut(s) | 78 |
| AjnI | CCWGG | 3 cut(s) | 14, 53, 274 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 104, 136 |
| AluBI | AGCT | 3 cut(s) | 62, 346, 418 |
| AluI | AGCT | 3 cut(s) | 62, 346, 418 |
| AlwI | GGATC | 3 cut(s) | 40, 177, 190 |
| Ama87I | CYCGRG | 2 cut(s) | 218, 388 |
| AoxI | GGCC | 4 cut(s) | 88, 192, 350, 372 |
| ApeKI | GCWGC | 1 cut(s) | 403 |
| AsiGI | ACCGGT | 1 cut(s) | 78 |
| AspLEI | GCGC | 1 cut(s) | 403 |
| AspS9I | GGNCC | 3 cut(s) | 120, 193, 258 |
| AsuC2I | CCSGG | 4 cut(s) | 92, 197, 389, 390 |
| AsuHPI | GGTGA | 1 cut(s) | 179 |
| AvaI | CYCGRG | 2 cut(s) | 218, 388 |
| AvaII | GGWCC | 2 cut(s) | 120, 258 |
| BamHI | GGATCC | 1 cut(s) | 182 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 224, 256 |
| BauI | CACGAG | 1 cut(s) | 407 |
| BbsI | GAAGAC | 1 cut(s) | 39 |
| BbvI | GCAGC | 1 cut(s) | 390 |
| BccI | CCATC | 3 cut(s) | 248, 356, 364 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 303, 337 |
| BciT130I | CCWGG | 3 cut(s) | 16, 55, 276 |
| BciVI | GTATCC | 2 cut(s) | 19, 321 |
| BcnI | CCSGG | 4 cut(s) | 92, 197, 389, 390 |
| BfoI | RGCGCY | 1 cut(s) | 404 |
| BfuI | GTATCC | 2 cut(s) | 19, 321 |
| BisI | GCNGC | 3 cut(s) | 353, 375, 404 |
| BlsI | GCNGC | 3 cut(s) | 354, 376, 405 |
| Bme1390I | CCNGG | 7 cut(s) | 16, 55, 92, 197, 276, 389, 390 |
| Bme18I | GGWCC | 2 cut(s) | 120, 258 |
| BmeT110I | CYCGRG | 2 cut(s) | 218, 388 |
| BmgT120I | GGNCC | 3 cut(s) | 120, 193, 258 |
| BmiI | GGNNCC | 4 cut(s) | 121, 184, 195, 259 |
| BmrFI | CCNGG | 7 cut(s) | 16, 55, 92, 197, 276, 389, 390 |
| BmrI | ACTGGG | 1 cut(s) | 138 |
| BmuI | ACTGGG | 1 cut(s) | 138 |
| BpiI | GAAGAC | 1 cut(s) | 39 |
| Bpu10I | CCTNAGC | 2 cut(s) | 347, 356 |
| BpuMI | CCSGG | 4 cut(s) | 92, 197, 389, 390 |
| BsaJI | CCNNGG | 8 cut(s) | 15, 186, 195, 219, 275, 387, 388, 389 |
| BsaWI | WCCGGW | 2 cut(s) | 78, 331 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 21, 129, 389, 393, 394 |
| Bse118I | RCCGGY | 1 cut(s) | 78 |
| Bse1I | ACTGG | 2 cut(s) | 43, 133 |
| BseBI | CCWGG | 3 cut(s) | 16, 55, 276 |
| BseDI | CCNNGG | 8 cut(s) | 15, 186, 195, 219, 275, 387, 388, 389 |
| BseGI | GGATG | 2 cut(s) | 259, 367 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 21, 129, 389, 393, 394 |
| BseNI | ACTGG | 2 cut(s) | 43, 133 |
| BseRI | GAGGAG | 1 cut(s) | 167 |
| BseXI | GCAGC | 1 cut(s) | 390 |
| BsgI | GTGCAG | 1 cut(s) | 389 |
| Bsh1236I | CGCG | 3 cut(s) | 188, 338, 377 |
| Bsh1285I | CGRYCG | 1 cut(s) | 79 |
| BshFI | GGCC | 4 cut(s) | 90, 194, 352, 374 |
| BshTI | ACCGGT | 1 cut(s) | 78 |
| BsiEI | CGRYCG | 1 cut(s) | 79 |
| BsiHKCI | CYCGRG | 2 cut(s) | 218, 388 |
| BsiSI | CCGG | 5 cut(s) | 79, 91, 197, 332, 389 |
| BslFI | GGGAC | 1 cut(s) | 271 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 21, 129, 389, 393, 394 |
| BsmFI | GGGAC | 1 cut(s) | 271 |
| BsmI | GAATGC | 1 cut(s) | 225 |
| BsnI | GGCC | 4 cut(s) | 90, 194, 352, 374 |
| BsoBI | CYCGRG | 2 cut(s) | 218, 388 |
| Bsp143I | GATC | 4 cut(s) | 32, 97, 182, 300 |
| BspACI | CCGC | 7 cut(s) | 107, 186, 188, 324, 338, 353, 375 |
| BspANI | GGCC | 4 cut(s) | 90, 194, 352, 374 |
| BspFNI | CGCG | 3 cut(s) | 188, 338, 377 |
| BspHI | TCATGA | 1 cut(s) | 285 |
| BspLI | GGNNCC | 4 cut(s) | 121, 184, 195, 259 |
| BspPI | GGATC | 3 cut(s) | 40, 177, 190 |
| BsrFI | RCCGGY | 1 cut(s) | 78 |
| BsrI | ACTGG | 2 cut(s) | 43, 133 |
| BssAI | RCCGGY | 1 cut(s) | 78 |
| BssECI | CCNNGG | 8 cut(s) | 15, 186, 195, 219, 275, 387, 388, 389 |
| BssMI | GATC | 4 cut(s) | 32, 97, 182, 300 |
| BssSI | CACGAG | 1 cut(s) | 407 |
| Bst2BI | CACGAG | 1 cut(s) | 407 |
| Bst2UI | CCWGG | 3 cut(s) | 16, 55, 276 |
| Bst4CI | ACNGT | 1 cut(s) | 208 |
| BstC8I | GCNNGC | 1 cut(s) | 60 |
| BstDEI | CTNAG | 3 cut(s) | 85, 347, 356 |
| BstDSI | CCRYGG | 1 cut(s) | 186 |
| BstF5I | GGATG | 2 cut(s) | 259, 367 |
| BstFNI | CGCG | 3 cut(s) | 188, 338, 377 |
| BstH2I | RGCGCY | 1 cut(s) | 404 |
| BstHHI | GCGC | 1 cut(s) | 403 |
| BstKTI | GATC | 4 cut(s) | 35, 100, 185, 303 |
| BstMBI | GATC | 4 cut(s) | 32, 97, 182, 300 |
| BstMCI | CGRYCG | 1 cut(s) | 79 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 59, 352 |
| BstNI | CCWGG | 3 cut(s) | 16, 55, 276 |
| BstNSI | RCATGY | 1 cut(s) | 313 |
| BstSCI | CCNGG | 7 cut(s) | 14, 53, 90, 195, 274, 387, 388 |
| BstUI | CGCG | 3 cut(s) | 188, 338, 377 |
| BstV1I | GCAGC | 1 cut(s) | 390 |
| BstV2I | GAAGAC | 1 cut(s) | 39 |
| BstX2I | RGATCY | 1 cut(s) | 182 |
| BstYI | RGATCY | 1 cut(s) | 182 |
| BsuI | GTATCC | 2 cut(s) | 19, 321 |
| BsuRI | GGCC | 4 cut(s) | 90, 194, 352, 374 |
| BtgI | CCRYGG | 1 cut(s) | 186 |
| BtsCI | GGATG | 2 cut(s) | 259, 367 |
| BtsIMutI | CAGTG | 1 cut(s) | 213 |
| Cac8I | GCNNGC | 1 cut(s) | 60 |
| CciI | TCATGA | 1 cut(s) | 285 |
| CfoI | GCGC | 1 cut(s) | 403 |
| Cfr10I | RCCGGY | 1 cut(s) | 78 |
| Cfr13I | GGNCC | 3 cut(s) | 120, 193, 258 |
| Cfr42I | CCGCGG | 1 cut(s) | 189 |
| Cfr9I | CCCGGG | 1 cut(s) | 388 |
| CseI | GACGC | 1 cut(s) | 344 |
| Csp6I | GTAC | 3 cut(s) | 40, 82, 241 |
| CspAI | ACCGGT | 1 cut(s) | 78 |
| CviAII | CATG | 2 cut(s) | 286, 310 |
| CviJI | RGCY | 8 cut(s) | 14, 62, 90, 194, 346, 352, 374, 418 |
| CviKI_1 | RGCY | 8 cut(s) | 14, 62, 90, 194, 346, 352, 374, 418 |
| CviQI | GTAC | 3 cut(s) | 40, 82, 241 |
| DdeI | CTNAG | 3 cut(s) | 85, 347, 356 |
| DpnI | GATC | 4 cut(s) | 34, 99, 184, 302 |
| DpnII | GATC | 4 cut(s) | 32, 97, 182, 300 |
| EaeI | YGGCCR | 1 cut(s) | 372 |
| Eco32I | GATATC | 1 cut(s) | 73 |
| Eco47I | GGWCC | 2 cut(s) | 120, 258 |
| Eco57I | CTGAAG | 2 cut(s) | 84, 338 |
| Eco88I | CYCGRG | 2 cut(s) | 218, 388 |
| EcoO109I | RGGNCCY | 2 cut(s) | 120, 193 |
| EcoRII | CCWGG | 3 cut(s) | 14, 53, 274 |
| EcoRV | GATATC | 1 cut(s) | 73 |
| FaeI | CATG | 2 cut(s) | 289, 313 |
| FaiI | YATR | 4 cut(s) | 263, 265, 287, 311 |
| FaqI | GGGAC | 1 cut(s) | 271 |
| FatI | CATG | 2 cut(s) | 285, 309 |
| FauI | CCCGC | 1 cut(s) | 317 |
| FauNDI | CATATG | 1 cut(s) | 263 |
| Fnu4HI | GCNGC | 3 cut(s) | 353, 375, 404 |
| FokI | GGATG | 2 cut(s) | 266, 374 |
| Fsp4HI | GCNGC | 3 cut(s) | 353, 375, 404 |
| GlaI | GCGC | 1 cut(s) | 402 |
| GluI | GCNGC | 3 cut(s) | 353, 375, 404 |
| HaeII | RGCGCY | 1 cut(s) | 404 |
| HaeIII | GGCC | 4 cut(s) | 90, 194, 352, 374 |
| HapII | CCGG | 5 cut(s) | 79, 91, 197, 332, 389 |
| HgaI | GACGC | 1 cut(s) | 344 |
| HhaI | GCGC | 1 cut(s) | 403 |
| Hin1II | CATG | 2 cut(s) | 289, 313 |
| Hin6I | GCGC | 1 cut(s) | 401 |
| HinP1I | GCGC | 1 cut(s) | 401 |
| HinfI | GANTC | 5 cut(s) | 5, 155, 282, 379, 412 |
| HpaII | CCGG | 5 cut(s) | 79, 91, 197, 332, 389 |
| HphI | GGTGA | 1 cut(s) | 179 |
| Hpy166II | GTNNAC | 2 cut(s) | 211, 243 |
| Hpy188I | TCNGA | 4 cut(s) | 10, 142, 160, 231 |
| Hpy188III | TCNNGA | 2 cut(s) | 218, 286 |
| Hpy8I | GTNNAC | 2 cut(s) | 211, 243 |
| Hpy99I | CGWCG | 2 cut(s) | 40, 272 |
| HpyAV | CCTTC | 1 cut(s) | 289 |
| HpyCH4III | ACNGT | 1 cut(s) | 208 |
| HpyCH4V | TGCA | 1 cut(s) | 406 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 59, 352 |
| HpyF3I | CTNAG | 3 cut(s) | 85, 347, 356 |
| Hsp92II | CATG | 2 cut(s) | 289, 313 |
| HspAI | GCGC | 1 cut(s) | 401 |
| KspI | CCGCGG | 1 cut(s) | 189 |
| Kzo9I | GATC | 4 cut(s) | 32, 97, 182, 300 |
| LmnI | GCTCC | 2 cut(s) | 343, 423 |
| Lsp1109I | GCAGC | 1 cut(s) | 390 |
| MaeIII | GTNAC | 1 cut(s) | 202 |
| MalI | GATC | 4 cut(s) | 34, 99, 184, 302 |
| MboI | GATC | 4 cut(s) | 32, 97, 182, 300 |
| MboII | GAAGA | 1 cut(s) | 39 |
| MflI | RGATCY | 1 cut(s) | 182 |
| MluCI | AATT | 1 cut(s) | 101 |
| MlyI | GAGTC | 1 cut(s) | 164 |
| MmeI | TCCRAC | 1 cut(s) | 183 |
| MnlI | CCTC | 5 cut(s) | 133, 145, 184, 214, 231 |
| MslI | CAYNNNNRTG | 2 cut(s) | 290, 308 |
| MspA1I | CMGCKG | 1 cut(s) | 188 |
| MspI | CCGG | 5 cut(s) | 79, 91, 197, 332, 389 |
| MspR9I | CCNGG | 7 cut(s) | 16, 55, 92, 197, 276, 389, 390 |
| Mva1269I | GAATGC | 1 cut(s) | 225 |
| MvaI | CCWGG | 3 cut(s) | 16, 55, 276 |
| MvnI | CGCG | 3 cut(s) | 188, 338, 377 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 59, 352 |
| NciI | CCSGG | 4 cut(s) | 92, 197, 389, 390 |
| NdeI | CATATG | 1 cut(s) | 263 |
| NdeII | GATC | 4 cut(s) | 32, 97, 182, 300 |
| NlaIII | CATG | 2 cut(s) | 289, 313 |
| NlaIV | GGNNCC | 4 cut(s) | 121, 184, 195, 259 |
| NmuCI | GTSAC | 1 cut(s) | 202 |
| NspI | RCATGY | 1 cut(s) | 313 |
| PagI | TCATGA | 1 cut(s) | 285 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 35, 122 |
| PctI | GAATGC | 1 cut(s) | 225 |
| PfeI | GAWTC | 4 cut(s) | 5, 282, 379, 412 |
| PinAI | ACCGGT | 1 cut(s) | 78 |
| PkrI | GCNGC | 3 cut(s) | 354, 376, 405 |
| PleI | GAGTC | 1 cut(s) | 163 |
| PpsI | GAGTC | 1 cut(s) | 163 |
| PpuMI | RGGWCCY | 1 cut(s) | 120 |
| Psp5II | RGGWCCY | 1 cut(s) | 120 |
| Psp6I | CCWGG | 3 cut(s) | 14, 53, 274 |
| PspGI | CCWGG | 3 cut(s) | 14, 53, 274 |
| PspN4I | GGNNCC | 4 cut(s) | 121, 184, 195, 259 |
| PspPI | GGNCC | 3 cut(s) | 120, 193, 258 |
| PspPPI | RGGWCCY | 1 cut(s) | 120 |
| PsuI | RGATCY | 1 cut(s) | 182 |
| RsaI | GTAC | 3 cut(s) | 41, 83, 242 |
| RsaNI | GTAC | 3 cut(s) | 40, 82, 241 |
| RseI | CAYNNNNRTG | 2 cut(s) | 290, 308 |
| SacII | CCGCGG | 1 cut(s) | 189 |
| SatI | GCNGC | 3 cut(s) | 353, 375, 404 |
| Sau3AI | GATC | 4 cut(s) | 32, 97, 182, 300 |
| Sau96I | GGNCC | 3 cut(s) | 120, 193, 258 |
| SchI | GAGTC | 1 cut(s) | 164 |
| ScrFI | CCNGG | 7 cut(s) | 16, 55, 92, 197, 276, 389, 390 |
| SetI | ASST | 5 cut(s) | 64, 242, 277, 348, 420 |
| Sfr303I | CCGCGG | 1 cut(s) | 189 |
| SgrBI | CCGCGG | 1 cut(s) | 189 |
| SinI | GGWCC | 2 cut(s) | 120, 258 |
| SmaI | CCCGGG | 1 cut(s) | 390 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 290, 308 |
| Sse9I | AATT | 1 cut(s) | 101 |
| SsiI | CCGC | 7 cut(s) | 107, 186, 188, 324, 338, 353, 375 |
| StyD4I | CCNGG | 7 cut(s) | 14, 53, 90, 195, 274, 387, 388 |
| TaaI | ACNGT | 1 cut(s) | 208 |
| TaqI | TCGA | 4 cut(s) | 75, 125, 236, 306 |
| TasI | AATT | 1 cut(s) | 101 |
| TauI | GCSGC | 2 cut(s) | 355, 377 |
| TfiI | GAWTC | 4 cut(s) | 5, 282, 379, 412 |
| TscAI | CASTG | 1 cut(s) | 213 |
| TseFI | GTSAC | 1 cut(s) | 202 |
| TseI | GCWGC | 1 cut(s) | 403 |
| Tsp45I | GTSAC | 1 cut(s) | 202 |
| TspDTI | ATGAA | 1 cut(s) | 308 |
| TspGWI | ACGGA | 1 cut(s) | 266 |
| TspMI | CCCGGG | 1 cut(s) | 388 |
| TspRI | CASTG | 1 cut(s) | 213 |
| VpaK11BI | GGWCC | 2 cut(s) | 120, 258 |
| XceI | RCATGY | 1 cut(s) | 313 |
| XmaI | CCCGGG | 1 cut(s) | 388 |