No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 163 |
| AccB1I | GGYRCC | 1 cut(s) | 162 |
| AccII | CGCG | 1 cut(s) | 160 |
| AciI | CCGC | 3 cut(s) | 53, 99, 160 |
| AclWI | GGATC | 3 cut(s) | 341, 354, 451 |
| AcyI | GRCGYC | 1 cut(s) | 406 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 80, 108, 182, 366, 387 |
| AjnI | CCWGG | 1 cut(s) | 270 |
| AluBI | AGCT | 1 cut(s) | 429 |
| AluI | AGCT | 1 cut(s) | 429 |
| Alw26I | GTCTC | 1 cut(s) | 59 |
| AlwI | GGATC | 3 cut(s) | 341, 354, 451 |
| Ama87I | CYCGRG | 2 cut(s) | 78, 103 |
| AspLEI | GCGC | 1 cut(s) | 160 |
| AspS9I | GGNCC | 2 cut(s) | 268, 376 |
| AsuC2I | CCSGG | 3 cut(s) | 79, 80, 344 |
| AsuHPI | GGTGA | 1 cut(s) | 286 |
| AvaI | CYCGRG | 2 cut(s) | 78, 103 |
| AvaII | GGWCC | 2 cut(s) | 268, 376 |
| BamHI | GGATCC | 1 cut(s) | 346 |
| BanI | GGYRCC | 1 cut(s) | 162 |
| BanII | GRGCYC | 1 cut(s) | 360 |
| BbsI | GAAGAC | 2 cut(s) | 201, 290 |
| BccI | CCATC | 7 cut(s) | 80, 82, 102, 214, 368, 407, 468 |
| BciT130I | CCWGG | 1 cut(s) | 272 |
| BciVI | GTATCC | 2 cut(s) | 36, 326 |
| BcnI | CCSGG | 3 cut(s) | 79, 80, 344 |
| BcoDI | GTCTC | 1 cut(s) | 59 |
| BfuAI | ACCTGC | 1 cut(s) | 163 |
| BfuI | GTATCC | 2 cut(s) | 36, 326 |
| Bme1390I | CCNGG | 4 cut(s) | 79, 80, 272, 344 |
| Bme18I | GGWCC | 2 cut(s) | 268, 376 |
| BmeT110I | CYCGRG | 2 cut(s) | 78, 103 |
| BmgT120I | GGNCC | 2 cut(s) | 268, 376 |
| BmiI | GGNNCC | 4 cut(s) | 164, 348, 378, 442 |
| BmrFI | CCNGG | 4 cut(s) | 79, 80, 272, 344 |
| BmsI | GCATC | 1 cut(s) | 40 |
| BpiI | GAAGAC | 2 cut(s) | 201, 290 |
| BpuMI | CCSGG | 3 cut(s) | 79, 80, 344 |
| BsaHI | GRCGYC | 1 cut(s) | 406 |
| BsaI | GGTCTC | 1 cut(s) | 59 |
| BsaJI | CCNNGG | 4 cut(s) | 78, 79, 102, 176 |
| BsaWI | WCCGGW | 3 cut(s) | 46, 287, 313 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 80, 108, 182, 366, 387 |
| BseBI | CCWGG | 1 cut(s) | 272 |
| BseDI | CCNNGG | 4 cut(s) | 78, 79, 102, 176 |
| BseGI | GGATG | 5 cut(s) | 55, 74, 113, 207, 331 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 80, 108, 182, 366, 387 |
| BseRI | GAGGAG | 1 cut(s) | 317 |
| Bsh1236I | CGCG | 1 cut(s) | 160 |
| BshNI | GGYRCC | 1 cut(s) | 162 |
| BsiHKCI | CYCGRG | 2 cut(s) | 78, 103 |
| BsiSI | CCGG | 5 cut(s) | 47, 79, 288, 314, 344 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 80, 108, 182, 366, 387 |
| BsmAI | GTCTC | 1 cut(s) | 59 |
| Bso31I | GGTCTC | 1 cut(s) | 59 |
| BsoBI | CYCGRG | 2 cut(s) | 78, 103 |
| Bsp1286I | GDGCHC | 1 cut(s) | 360 |
| Bsp143I | GATC | 4 cut(s) | 15, 297, 346, 456 |
| BspACI | CCGC | 3 cut(s) | 53, 99, 160 |
| BspFNI | CGCG | 1 cut(s) | 160 |
| BspLI | GGNNCC | 4 cut(s) | 164, 348, 378, 442 |
| BspMI | ACCTGC | 1 cut(s) | 163 |
| BspPI | GGATC | 3 cut(s) | 341, 354, 451 |
| BspT107I | GGYRCC | 1 cut(s) | 162 |
| BspTNI | GGTCTC | 1 cut(s) | 59 |
| BssECI | CCNNGG | 4 cut(s) | 78, 79, 102, 176 |
| BssMI | GATC | 4 cut(s) | 15, 297, 346, 456 |
| BssNI | GRCGYC | 1 cut(s) | 406 |
| Bst2UI | CCWGG | 1 cut(s) | 272 |
| Bst4CI | ACNGT | 1 cut(s) | 177 |
| BstACI | GRCGYC | 1 cut(s) | 406 |
| BstC8I | GCNNGC | 1 cut(s) | 253 |
| BstDEI | CTNAG | 2 cut(s) | 279, 471 |
| BstDSI | CCRYGG | 1 cut(s) | 176 |
| BstF5I | GGATG | 5 cut(s) | 55, 74, 113, 207, 331 |
| BstFNI | CGCG | 1 cut(s) | 160 |
| BstHHI | GCGC | 1 cut(s) | 160 |
| BstKTI | GATC | 4 cut(s) | 18, 300, 349, 459 |
| BstMAI | GTCTC | 1 cut(s) | 59 |
| BstMBI | GATC | 4 cut(s) | 15, 297, 346, 456 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 157 |
| BstNI | CCWGG | 1 cut(s) | 272 |
| BstSCI | CCNGG | 4 cut(s) | 77, 78, 270, 342 |
| BstUI | CGCG | 1 cut(s) | 160 |
| BstV2I | GAAGAC | 2 cut(s) | 201, 290 |
| BstX2I | RGATCY | 1 cut(s) | 346 |
| BstYI | RGATCY | 1 cut(s) | 346 |
| BsuI | GTATCC | 2 cut(s) | 36, 326 |
| BtgI | CCRYGG | 1 cut(s) | 176 |
| BtsCI | GGATG | 5 cut(s) | 55, 74, 113, 207, 331 |
| BveI | ACCTGC | 1 cut(s) | 163 |
| Cac8I | GCNNGC | 1 cut(s) | 253 |
| CfoI | GCGC | 1 cut(s) | 160 |
| Cfr13I | GGNCC | 2 cut(s) | 268, 376 |
| Cfr9I | CCCGGG | 1 cut(s) | 78 |
| CseI | GACGC | 1 cut(s) | 414 |
| CsiI | ACCWGGT | 1 cut(s) | 270 |
| CviAII | CATG | 3 cut(s) | 122, 137, 226 |
| CviJI | RGCY | 2 cut(s) | 358, 429 |
| CviKI_1 | RGCY | 2 cut(s) | 358, 429 |
| DdeI | CTNAG | 2 cut(s) | 279, 471 |
| DpnI | GATC | 4 cut(s) | 17, 299, 348, 458 |
| DpnII | GATC | 4 cut(s) | 15, 297, 346, 456 |
| EciI | GGCGGA | 1 cut(s) | 88 |
| Eco24I | GRGCYC | 1 cut(s) | 360 |
| Eco31I | GGTCTC | 1 cut(s) | 59 |
| Eco32I | GATATC | 1 cut(s) | 321 |
| Eco47I | GGWCC | 2 cut(s) | 268, 376 |
| Eco88I | CYCGRG | 2 cut(s) | 78, 103 |
| EcoO109I | RGGNCCY | 1 cut(s) | 376 |
| EcoRII | CCWGG | 1 cut(s) | 270 |
| EcoRV | GATATC | 1 cut(s) | 321 |
| EcoT38I | GRGCYC | 1 cut(s) | 360 |
| FaeI | CATG | 3 cut(s) | 125, 140, 229 |
| FaiI | YATR | 7 cut(s) | 8, 123, 138, 227, 233, 249, 387 |
| FatI | CATG | 3 cut(s) | 121, 136, 225 |
| FokI | GGATG | 5 cut(s) | 61, 62, 120, 214, 338 |
| FriOI | GRGCYC | 1 cut(s) | 360 |
| GlaI | GCGC | 1 cut(s) | 159 |
| HapII | CCGG | 5 cut(s) | 47, 79, 288, 314, 344 |
| HgaI | GACGC | 1 cut(s) | 414 |
| HhaI | GCGC | 1 cut(s) | 160 |
| Hin1I | GRCGYC | 1 cut(s) | 406 |
| Hin1II | CATG | 3 cut(s) | 125, 140, 229 |
| Hin6I | GCGC | 1 cut(s) | 158 |
| HinP1I | GCGC | 1 cut(s) | 158 |
| HinfI | GANTC | 2 cut(s) | 95, 235 |
| HpaII | CCGG | 5 cut(s) | 47, 79, 288, 314, 344 |
| HphI | GGTGA | 1 cut(s) | 286 |
| Hpy166II | GTNNAC | 1 cut(s) | 268 |
| Hpy188I | TCNGA | 2 cut(s) | 325, 369 |
| Hpy188III | TCNNGA | 3 cut(s) | 105, 350, 454 |
| Hpy8I | GTNNAC | 1 cut(s) | 268 |
| Hpy99I | CGWCG | 1 cut(s) | 408 |
| HpyAV | CCTTC | 4 cut(s) | 4, 134, 175, 412 |
| HpyCH4III | ACNGT | 1 cut(s) | 177 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 157 |
| HpyF3I | CTNAG | 2 cut(s) | 279, 471 |
| Hsp92I | GRCGYC | 1 cut(s) | 406 |
| Hsp92II | CATG | 3 cut(s) | 125, 140, 229 |
| HspAI | GCGC | 1 cut(s) | 158 |
| Kzo9I | GATC | 4 cut(s) | 15, 297, 346, 456 |
| LpnPI | CCDG | 8 cut(s) | 60, 92, 168, 257, 284, 301, 327, 357 |
| LweI | GCATC | 1 cut(s) | 40 |
| MabI | ACCWGGT | 1 cut(s) | 270 |
| MaeIII | GTNAC | 2 cut(s) | 274, 398 |
| MalI | GATC | 4 cut(s) | 17, 299, 348, 458 |
| MboI | GATC | 4 cut(s) | 15, 297, 346, 456 |
| MboII | GAAGA | 2 cut(s) | 206, 295 |
| MflI | RGATCY | 1 cut(s) | 346 |
| MhlI | GDGCHC | 1 cut(s) | 360 |
| MluCI | AATT | 1 cut(s) | 215 |
| MslI | CAYNNNNRTG | 3 cut(s) | 230, 261, 263 |
| MspI | CCGG | 5 cut(s) | 47, 79, 288, 314, 344 |
| MspR9I | CCNGG | 4 cut(s) | 79, 80, 272, 344 |
| MvaI | CCWGG | 1 cut(s) | 272 |
| MvnI | CGCG | 1 cut(s) | 160 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 157 |
| NciI | CCSGG | 3 cut(s) | 79, 80, 344 |
| NdeII | GATC | 4 cut(s) | 15, 297, 346, 456 |
| NlaIII | CATG | 3 cut(s) | 125, 140, 229 |
| NlaIV | GGNNCC | 4 cut(s) | 164, 348, 378, 442 |
| NmeAIII | GCCGAG | 1 cut(s) | 434 |
| NmuCI | GTSAC | 1 cut(s) | 274 |
| PfeI | GAWTC | 2 cut(s) | 95, 235 |
| PpuMI | RGGWCCY | 1 cut(s) | 376 |
| Psp5II | RGGWCCY | 1 cut(s) | 376 |
| Psp6I | CCWGG | 1 cut(s) | 270 |
| PspGI | CCWGG | 1 cut(s) | 270 |
| PspN4I | GGNNCC | 4 cut(s) | 164, 348, 378, 442 |
| PspPI | GGNCC | 2 cut(s) | 268, 376 |
| PspPPI | RGGWCCY | 1 cut(s) | 376 |
| PsuI | RGATCY | 1 cut(s) | 346 |
| RseI | CAYNNNNRTG | 3 cut(s) | 230, 261, 263 |
| Sau3AI | GATC | 4 cut(s) | 15, 297, 346, 456 |
| Sau96I | GGNCC | 2 cut(s) | 268, 376 |
| ScrFI | CCNGG | 4 cut(s) | 79, 80, 272, 344 |
| SduI | GDGCHC | 1 cut(s) | 360 |
| SetI | ASST | 4 cut(s) | 71, 157, 273, 431 |
| SexAI | ACCWGGT | 1 cut(s) | 270 |
| SfaNI | GCATC | 1 cut(s) | 40 |
| SinI | GGWCC | 2 cut(s) | 268, 376 |
| SmaI | CCCGGG | 1 cut(s) | 80 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 230, 261, 263 |
| Sse9I | AATT | 1 cut(s) | 215 |
| SsiI | CCGC | 3 cut(s) | 53, 99, 160 |
| StyD4I | CCNGG | 4 cut(s) | 77, 78, 270, 342 |
| TaaI | ACNGT | 1 cut(s) | 177 |
| TaqI | TCGA | 2 cut(s) | 300, 455 |
| TasI | AATT | 1 cut(s) | 215 |
| TfiI | GAWTC | 2 cut(s) | 95, 235 |
| TseFI | GTSAC | 1 cut(s) | 274 |
| Tsp45I | GTSAC | 1 cut(s) | 274 |
| TspDTI | ATGAA | 5 cut(s) | 23, 138, 153, 218, 248 |
| TspGWI | ACGGA | 1 cut(s) | 184 |
| TspMI | CCCGGG | 1 cut(s) | 78 |
| VpaK11BI | GGWCC | 2 cut(s) | 268, 376 |
| XmaI | CCCGGG | 1 cut(s) | 78 |