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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 278 |
| AclWI | GGATC | 3 cut(s) | 310, 320, 323 |
| AcoI | YGGCCR | 1 cut(s) | 208 |
| AfaI | GTAC | 1 cut(s) | 357 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 217 |
| AgsI | TTSAA | 4 cut(s) | 30, 150, 163, 401 |
| AjnI | CCWGG | 1 cut(s) | 210 |
| Alw26I | GTCTC | 2 cut(s) | 218, 344 |
| AlwI | GGATC | 3 cut(s) | 310, 320, 323 |
| AoxI | GGCC | 3 cut(s) | 208, 230, 295 |
| ApeKI | GCWGC | 1 cut(s) | 412 |
| AspS9I | GGNCC | 2 cut(s) | 230, 296 |
| AsuHPI | GGTGA | 3 cut(s) | 50, 233, 385 |
| AsuII | TTCGAA | 1 cut(s) | 77 |
| BalI | TGGCCA | 1 cut(s) | 210 |
| BamHI | GGATCC | 1 cut(s) | 315 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 33, 65 |
| BbvI | GCAGC | 1 cut(s) | 399 |
| BciT130I | CCWGG | 1 cut(s) | 212 |
| BcoDI | GTCTC | 2 cut(s) | 218, 344 |
| BfaI | CTAG | 3 cut(s) | 60, 116, 141 |
| BfmI | CTRYAG | 1 cut(s) | 413 |
| BisI | GCNGC | 1 cut(s) | 413 |
| BlsI | GCNGC | 1 cut(s) | 414 |
| Bme1390I | CCNGG | 1 cut(s) | 212 |
| BmgT120I | GGNCC | 2 cut(s) | 230, 296 |
| BmiI | GGNNCC | 2 cut(s) | 298, 317 |
| BmrFI | CCNGG | 1 cut(s) | 212 |
| BmrI | ACTGGG | 2 cut(s) | 237, 388 |
| BmuI | ACTGGG | 2 cut(s) | 237, 388 |
| Bpu10I | CCTNAGC | 1 cut(s) | 333 |
| Bpu14I | TTCGAA | 1 cut(s) | 77 |
| BsaBI | GATNNNNATC | 1 cut(s) | 53 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 217 |
| Bse1I | ACTGG | 2 cut(s) | 232, 383 |
| Bse8I | GATNNNNATC | 1 cut(s) | 53 |
| BseBI | CCWGG | 1 cut(s) | 212 |
| BseJI | GATNNNNATC | 1 cut(s) | 53 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 217 |
| BseMII | CTCAG | 1 cut(s) | 324 |
| BseNI | ACTGG | 2 cut(s) | 232, 383 |
| BseXI | GCAGC | 1 cut(s) | 399 |
| BsgI | GTGCAG | 1 cut(s) | 312 |
| BshFI | GGCC | 3 cut(s) | 210, 232, 297 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 217 |
| BsmAI | GTCTC | 2 cut(s) | 218, 344 |
| BsnI | GGCC | 3 cut(s) | 210, 232, 297 |
| Bsp119I | TTCGAA | 1 cut(s) | 77 |
| Bsp143I | GATC | 2 cut(s) | 315, 325 |
| BspACI | CCGC | 1 cut(s) | 278 |
| BspANI | GGCC | 3 cut(s) | 210, 232, 297 |
| BspCNI | CTCAG | 1 cut(s) | 325 |
| BspLI | GGNNCC | 2 cut(s) | 298, 317 |
| BspMAI | CTGCAG | 1 cut(s) | 417 |
| BspPI | GGATC | 3 cut(s) | 310, 320, 323 |
| BspT104I | TTCGAA | 1 cut(s) | 77 |
| BsrI | ACTGG | 2 cut(s) | 232, 383 |
| BssMI | GATC | 2 cut(s) | 315, 325 |
| Bst2UI | CCWGG | 1 cut(s) | 212 |
| Bst4CI | ACNGT | 1 cut(s) | 355 |
| Bst6I | CTCTTC | 1 cut(s) | 210 |
| BstBI | TTCGAA | 1 cut(s) | 77 |
| BstC8I | GCNNGC | 2 cut(s) | 208, 295 |
| BstDEI | CTNAG | 1 cut(s) | 333 |
| BstKTI | GATC | 2 cut(s) | 318, 328 |
| BstMAI | GTCTC | 2 cut(s) | 218, 344 |
| BstMBI | GATC | 2 cut(s) | 315, 325 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 290 |
| BstNI | CCWGG | 1 cut(s) | 212 |
| BstSCI | CCNGG | 1 cut(s) | 210 |
| BstSFI | CTRYAG | 1 cut(s) | 413 |
| BstV1I | GCAGC | 1 cut(s) | 399 |
| BstX2I | RGATCY | 1 cut(s) | 315 |
| BstYI | RGATCY | 1 cut(s) | 315 |
| BsuRI | GGCC | 3 cut(s) | 210, 232, 297 |
| BtsI | GCAGTG | 1 cut(s) | 102 |
| BtsIMutI | CAGTG | 1 cut(s) | 102 |
| Cac8I | GCNNGC | 2 cut(s) | 208, 295 |
| Cfr13I | GGNCC | 2 cut(s) | 230, 296 |
| Csp6I | GTAC | 1 cut(s) | 356 |
| CviAII | CATG | 1 cut(s) | 320 |
| CviJI | RGCY | 8 cut(s) | 46, 125, 158, 206, 210, 232, 263, 297 |
| CviKI_1 | RGCY | 8 cut(s) | 46, 125, 158, 206, 210, 232, 263, 297 |
| CviQI | GTAC | 1 cut(s) | 356 |
| DdeI | CTNAG | 1 cut(s) | 333 |
| DpnI | GATC | 2 cut(s) | 317, 327 |
| DpnII | GATC | 2 cut(s) | 315, 325 |
| DraI | TTTAAA | 1 cut(s) | 267 |
| EaeI | YGGCCR | 1 cut(s) | 208 |
| Eam1104I | CTCTTC | 1 cut(s) | 210 |
| EarI | CTCTTC | 1 cut(s) | 210 |
| EcoO109I | RGGNCCY | 1 cut(s) | 296 |
| EcoRII | CCWGG | 1 cut(s) | 210 |
| FaeI | CATG | 1 cut(s) | 323 |
| FaiI | YATR | 5 cut(s) | 9, 17, 321, 344, 386 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 142, 174 |
| FatI | CATG | 1 cut(s) | 319 |
| FauI | CCCGC | 1 cut(s) | 271 |
| Fnu4HI | GCNGC | 1 cut(s) | 413 |
| Fsp4HI | GCNGC | 1 cut(s) | 413 |
| FspBI | CTAG | 3 cut(s) | 60, 116, 141 |
| GluI | GCNGC | 1 cut(s) | 413 |
| HaeIII | GGCC | 3 cut(s) | 210, 232, 297 |
| Hin1II | CATG | 1 cut(s) | 323 |
| HphI | GGTGA | 3 cut(s) | 50, 233, 385 |
| Hpy166II | GTNNAC | 1 cut(s) | 376 |
| Hpy188I | TCNGA | 1 cut(s) | 310 |
| Hpy188III | TCNNGA | 2 cut(s) | 116, 332 |
| Hpy8I | GTNNAC | 1 cut(s) | 376 |
| Hpy99I | CGWCG | 1 cut(s) | 242 |
| HpyAV | CCTTC | 3 cut(s) | 35, 196, 413 |
| HpyCH4III | ACNGT | 1 cut(s) | 355 |
| HpyCH4V | TGCA | 3 cut(s) | 199, 293, 415 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 290 |
| HpyF3I | CTNAG | 1 cut(s) | 333 |
| Hsp92II | CATG | 1 cut(s) | 323 |
| Kzo9I | GATC | 2 cut(s) | 315, 325 |
| Lsp1109I | GCAGC | 1 cut(s) | 399 |
| MaeI | CTAG | 3 cut(s) | 60, 116, 141 |
| MaeIII | GTNAC | 1 cut(s) | 62 |
| MalI | GATC | 2 cut(s) | 317, 327 |
| MboI | GATC | 2 cut(s) | 315, 325 |
| MboII | GAAGA | 2 cut(s) | 138, 227 |
| MflI | RGATCY | 1 cut(s) | 315 |
| MlsI | TGGCCA | 1 cut(s) | 210 |
| MluCI | AATT | 4 cut(s) | 30, 109, 181, 401 |
| MluNI | TGGCCA | 1 cut(s) | 210 |
| MnlI | CCTC | 5 cut(s) | 61, 211, 243, 310, 339 |
| Mox20I | TGGCCA | 1 cut(s) | 210 |
| MscI | TGGCCA | 1 cut(s) | 210 |
| MseI | TTAA | 3 cut(s) | 266, 360, 404 |
| Msp20I | TGGCCA | 1 cut(s) | 210 |
| MspR9I | CCNGG | 1 cut(s) | 212 |
| MvaI | CCWGG | 1 cut(s) | 212 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 290 |
| NdeII | GATC | 2 cut(s) | 315, 325 |
| NlaIII | CATG | 1 cut(s) | 323 |
| NlaIV | GGNNCC | 2 cut(s) | 298, 317 |
| NmuCI | GTSAC | 1 cut(s) | 62 |
| NspV | TTCGAA | 1 cut(s) | 77 |
| PkrI | GCNGC | 1 cut(s) | 414 |
| Psp6I | CCWGG | 1 cut(s) | 210 |
| PspGI | CCWGG | 1 cut(s) | 210 |
| PspN4I | GGNNCC | 2 cut(s) | 298, 317 |
| PspPI | GGNCC | 2 cut(s) | 230, 296 |
| PstI | CTGCAG | 1 cut(s) | 417 |
| PsuI | RGATCY | 1 cut(s) | 315 |
| RsaI | GTAC | 1 cut(s) | 357 |
| RsaNI | GTAC | 1 cut(s) | 356 |
| SaqAI | TTAA | 3 cut(s) | 266, 360, 404 |
| SatI | GCNGC | 1 cut(s) | 413 |
| Sau3AI | GATC | 2 cut(s) | 315, 325 |
| Sau96I | GGNCC | 2 cut(s) | 230, 296 |
| ScrFI | CCNGG | 1 cut(s) | 212 |
| SetI | ASST | 2 cut(s) | 169, 410 |
| SfcI | CTRYAG | 1 cut(s) | 413 |
| SfuI | TTCGAA | 1 cut(s) | 77 |
| Sse9I | AATT | 4 cut(s) | 30, 109, 181, 401 |
| SsiI | CCGC | 1 cut(s) | 278 |
| SspMI | CTAG | 3 cut(s) | 60, 116, 141 |
| StyD4I | CCNGG | 1 cut(s) | 210 |
| TaaI | ACNGT | 1 cut(s) | 355 |
| TaqI | TCGA | 1 cut(s) | 77 |
| TasI | AATT | 4 cut(s) | 30, 109, 181, 401 |
| TatI | WGTACW | 1 cut(s) | 355 |
| Tru1I | TTAA | 3 cut(s) | 266, 360, 404 |
| Tru9I | TTAA | 3 cut(s) | 266, 360, 404 |
| TscAI | CASTG | 1 cut(s) | 109 |
| TseFI | GTSAC | 1 cut(s) | 62 |
| TseI | GCWGC | 1 cut(s) | 412 |
| Tsp45I | GTSAC | 1 cut(s) | 62 |
| TspDTI | ATGAA | 1 cut(s) | 359 |
| TspRI | CASTG | 1 cut(s) | 109 |
| XbaI | TCTAGA | 1 cut(s) | 115 |
| XspI | CTAG | 3 cut(s) | 60, 116, 141 |