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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 472 |
| AccB1I | GGYRCC | 1 cut(s) | 225 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 19 |
| AccBSI | CCGCTC | 1 cut(s) | 329 |
| AccII | CGCG | 1 cut(s) | 320 |
| AciI | CCGC | 5 cut(s) | 255, 318, 329, 346, 397 |
| AclWI | GGATC | 4 cut(s) | 140, 202, 352, 410 |
| AcsI | RAATTY | 1 cut(s) | 104 |
| AcyI | GRCGYC | 2 cut(s) | 48, 226 |
| AfaI | GTAC | 2 cut(s) | 134, 494 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 19, 184, 217, 483 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 73 |
| AluBI | AGCT | 1 cut(s) | 421 |
| AluI | AGCT | 1 cut(s) | 421 |
| Alw21I | GWGCWC | 1 cut(s) | 317 |
| AlwI | GGATC | 4 cut(s) | 140, 202, 352, 410 |
| Ama87I | CYCGRG | 1 cut(s) | 165 |
| ApeKI | GCWGC | 2 cut(s) | 411, 473 |
| ApoI | RAATTY | 1 cut(s) | 104 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 105, 108, 304 |
| AspLEI | GCGC | 2 cut(s) | 228, 473 |
| AspS9I | GGNCC | 1 cut(s) | 252 |
| AsuC2I | CCSGG | 3 cut(s) | 212, 259, 449 |
| AsuHPI | GGTGA | 1 cut(s) | 304 |
| AvaI | CYCGRG | 1 cut(s) | 165 |
| AvaII | GGWCC | 1 cut(s) | 252 |
| BanI | GGYRCC | 1 cut(s) | 225 |
| BauI | CACGAG | 1 cut(s) | 310 |
| BbsI | GAAGAC | 1 cut(s) | 51 |
| Bbv12I | GWGCWC | 1 cut(s) | 317 |
| BbvI | GCAGC | 2 cut(s) | 398, 485 |
| BccI | CCATC | 1 cut(s) | 13 |
| BceAI | ACGGC | 2 cut(s) | 213, 399 |
| BcgI | CGANNNNNNTGC | 6 cut(s) | 7, 41, 134, 168, 304, 338 |
| BciVI | GTATCC | 1 cut(s) | 242 |
| BcnI | CCSGG | 3 cut(s) | 212, 259, 449 |
| BfoI | RGCGCY | 1 cut(s) | 229 |
| BfuI | GTATCC | 1 cut(s) | 242 |
| BisI | GCNGC | 2 cut(s) | 412, 474 |
| BlsI | GCNGC | 2 cut(s) | 413, 475 |
| Bme1390I | CCNGG | 3 cut(s) | 212, 259, 449 |
| Bme18I | GGWCC | 1 cut(s) | 252 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 73 |
| BmeT110I | CYCGRG | 1 cut(s) | 165 |
| BmgT120I | GGNCC | 1 cut(s) | 252 |
| BmiI | GGNNCC | 3 cut(s) | 140, 227, 307 |
| BmrFI | CCNGG | 3 cut(s) | 212, 259, 449 |
| BmsI | GCATC | 2 cut(s) | 362, 390 |
| BpiI | GAAGAC | 1 cut(s) | 51 |
| Bpu10I | CCTNAGC | 1 cut(s) | 377 |
| BpuMI | CCSGG | 3 cut(s) | 212, 259, 449 |
| BsaHI | GRCGYC | 2 cut(s) | 48, 226 |
| BsaJI | CCNNGG | 1 cut(s) | 37 |
| BsaWI | WCCGGW | 1 cut(s) | 249 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 19, 184, 217, 483 |
| Bse1I | ACTGG | 1 cut(s) | 13 |
| BseDI | CCNNGG | 1 cut(s) | 37 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 19, 184, 217, 483 |
| BseNI | ACTGG | 1 cut(s) | 13 |
| BseRI | GAGGAG | 1 cut(s) | 467 |
| BseXI | GCAGC | 2 cut(s) | 398, 485 |
| Bsh1236I | CGCG | 1 cut(s) | 320 |
| Bsh1285I | CGRYCG | 1 cut(s) | 324 |
| BshNI | GGYRCC | 1 cut(s) | 225 |
| BsiEI | CGRYCG | 1 cut(s) | 324 |
| BsiHKAI | GWGCWC | 1 cut(s) | 317 |
| BsiHKCI | CYCGRG | 1 cut(s) | 165 |
| BsiSI | CCGG | 7 cut(s) | 212, 235, 250, 258, 267, 400, 449 |
| BslFI | GGGAC | 1 cut(s) | 202 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 19, 184, 217, 483 |
| BsmFI | GGGAC | 1 cut(s) | 202 |
| BsoBI | CYCGRG | 1 cut(s) | 165 |
| Bsp1286I | GDGCHC | 1 cut(s) | 317 |
| Bsp143I | GATC | 5 cut(s) | 145, 207, 321, 357, 402 |
| BspACI | CCGC | 5 cut(s) | 255, 318, 329, 346, 397 |
| BspFNI | CGCG | 1 cut(s) | 320 |
| BspLI | GGNNCC | 3 cut(s) | 140, 227, 307 |
| BspPI | GGATC | 4 cut(s) | 140, 202, 352, 410 |
| BspT107I | GGYRCC | 1 cut(s) | 225 |
| BsrBI | CCGCTC | 1 cut(s) | 329 |
| BsrI | ACTGG | 1 cut(s) | 13 |
| BssECI | CCNNGG | 1 cut(s) | 37 |
| BssMI | GATC | 5 cut(s) | 145, 207, 321, 357, 402 |
| BssNI | GRCGYC | 2 cut(s) | 48, 226 |
| BssSI | CACGAG | 1 cut(s) | 310 |
| BssT1I | CCWWGG | 1 cut(s) | 37 |
| Bst2BI | CACGAG | 1 cut(s) | 310 |
| Bst4CI | ACNGT | 1 cut(s) | 73 |
| Bst6I | CTCTTC | 3 cut(s) | 251, 295, 335 |
| BstACI | GRCGYC | 2 cut(s) | 48, 226 |
| BstDEI | CTNAG | 1 cut(s) | 377 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 481 |
| BstFNI | CGCG | 1 cut(s) | 320 |
| BstH2I | RGCGCY | 1 cut(s) | 229 |
| BstHHI | GCGC | 2 cut(s) | 228, 473 |
| BstKTI | GATC | 5 cut(s) | 148, 210, 324, 360, 405 |
| BstMBI | GATC | 5 cut(s) | 145, 207, 321, 357, 402 |
| BstMCI | CGRYCG | 1 cut(s) | 324 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 263, 326 |
| BstSCI | CCNGG | 3 cut(s) | 210, 257, 447 |
| BstUI | CGCG | 1 cut(s) | 320 |
| BstV1I | GCAGC | 2 cut(s) | 398, 485 |
| BstV2I | GAAGAC | 1 cut(s) | 51 |
| BsuI | GTATCC | 1 cut(s) | 242 |
| CfoI | GCGC | 2 cut(s) | 228, 473 |
| Cfr13I | GGNCC | 1 cut(s) | 252 |
| CpoI | CGGWCCG | 1 cut(s) | 252 |
| CseI | GACGC | 2 cut(s) | 56, 199 |
| Csp6I | GTAC | 2 cut(s) | 133, 493 |
| CspI | CGGWCCG | 1 cut(s) | 252 |
| CviJI | RGCY | 7 cut(s) | 83, 177, 238, 266, 411, 421, 476 |
| CviKI_1 | RGCY | 7 cut(s) | 83, 177, 238, 266, 411, 421, 476 |
| CviQI | GTAC | 2 cut(s) | 133, 493 |
| DdeI | CTNAG | 1 cut(s) | 377 |
| DinI | GGCGCC | 1 cut(s) | 227 |
| DpnI | GATC | 5 cut(s) | 147, 209, 323, 359, 404 |
| DpnII | GATC | 5 cut(s) | 145, 207, 321, 357, 402 |
| DriI | GACNNNNNGTC | 1 cut(s) | 73 |
| Eam1104I | CTCTTC | 3 cut(s) | 251, 295, 335 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 73 |
| EarI | CTCTTC | 3 cut(s) | 251, 295, 335 |
| EciI | GGCGGA | 1 cut(s) | 244 |
| Eco130I | CCWWGG | 1 cut(s) | 37 |
| Eco47I | GGWCC | 1 cut(s) | 252 |
| Eco88I | CYCGRG | 1 cut(s) | 165 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 481 |
| EcoT14I | CCWWGG | 1 cut(s) | 37 |
| EgeI | GGCGCC | 1 cut(s) | 227 |
| EheI | GGCGCC | 1 cut(s) | 227 |
| ErhI | CCWWGG | 1 cut(s) | 37 |
| FaiI | YATR | 2 cut(s) | 26, 32 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 36, 68, 93, 125, 270, 302 |
| FaqI | GGGAC | 1 cut(s) | 202 |
| FauI | CCCGC | 1 cut(s) | 322 |
| Fnu4HI | GCNGC | 2 cut(s) | 412, 474 |
| Fsp4HI | GCNGC | 2 cut(s) | 412, 474 |
| FspI | TGCGCA | 1 cut(s) | 472 |
| GlaI | GCGC | 2 cut(s) | 227, 472 |
| GluI | GCNGC | 2 cut(s) | 412, 474 |
| HaeII | RGCGCY | 1 cut(s) | 229 |
| HapII | CCGG | 7 cut(s) | 212, 235, 250, 258, 267, 400, 449 |
| HgaI | GACGC | 2 cut(s) | 56, 199 |
| HhaI | GCGC | 2 cut(s) | 228, 473 |
| Hin1I | GRCGYC | 2 cut(s) | 48, 226 |
| Hin6I | GCGC | 2 cut(s) | 226, 471 |
| HinP1I | GCGC | 2 cut(s) | 226, 471 |
| HincII | GTYRAC | 1 cut(s) | 67 |
| HindII | GTYRAC | 1 cut(s) | 67 |
| HpaII | CCGG | 7 cut(s) | 212, 235, 250, 258, 267, 400, 449 |
| HphI | GGTGA | 1 cut(s) | 304 |
| Hpy166II | GTNNAC | 3 cut(s) | 67, 172, 493 |
| Hpy188I | TCNGA | 1 cut(s) | 326 |
| Hpy188III | TCNNGA | 2 cut(s) | 113, 385 |
| Hpy8I | GTNNAC | 3 cut(s) | 67, 172, 493 |
| HpyAV | CCTTC | 2 cut(s) | 62, 73 |
| HpyCH4III | ACNGT | 1 cut(s) | 73 |
| HpyCH4IV | ACGT | 1 cut(s) | 63 |
| HpyCH4V | TGCA | 1 cut(s) | 426 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 263, 326 |
| HpyF3I | CTNAG | 1 cut(s) | 377 |
| HpySE526I | ACGT | 1 cut(s) | 63 |
| Hsp92I | GRCGYC | 2 cut(s) | 48, 226 |
| HspAI | GCGC | 2 cut(s) | 226, 471 |
| KasI | GGCGCC | 1 cut(s) | 225 |
| Kzo9I | GATC | 5 cut(s) | 145, 207, 321, 357, 402 |
| LmnI | GCTCC | 1 cut(s) | 326 |
| LpnPI | CCDG | 9 cut(s) | 26, 225, 248, 263, 271, 275, 280, 413, 462 |
| Lsp1109I | GCAGC | 2 cut(s) | 398, 485 |
| LweI | GCATC | 2 cut(s) | 362, 390 |
| MaeII | ACGT | 1 cut(s) | 63 |
| MaeIII | GTNAC | 1 cut(s) | 160 |
| MalI | GATC | 5 cut(s) | 147, 209, 323, 359, 404 |
| MbiI | CCGCTC | 1 cut(s) | 329 |
| MboI | GATC | 5 cut(s) | 145, 207, 321, 357, 402 |
| MboII | GAAGA | 7 cut(s) | 56, 101, 113, 238, 309, 312, 352 |
| MhlI | GDGCHC | 1 cut(s) | 317 |
| MluCI | AATT | 1 cut(s) | 104 |
| Mly113I | GGCGCC | 1 cut(s) | 226 |
| MnlI | CCTC | 7 cut(s) | 118, 188, 211, 268, 296, 336, 445 |
| MroXI | GAANNNNTTC | 3 cut(s) | 105, 108, 304 |
| MseI | TTAA | 2 cut(s) | 393, 482 |
| MspI | CCGG | 7 cut(s) | 212, 235, 250, 258, 267, 400, 449 |
| MspR9I | CCNGG | 3 cut(s) | 212, 259, 449 |
| MvnI | CGCG | 1 cut(s) | 320 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 263, 326 |
| NarI | GGCGCC | 1 cut(s) | 226 |
| NciI | CCSGG | 3 cut(s) | 212, 259, 449 |
| NdeII | GATC | 5 cut(s) | 145, 207, 321, 357, 402 |
| NlaIV | GGNNCC | 3 cut(s) | 140, 227, 307 |
| NsbI | TGCGCA | 1 cut(s) | 472 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 141, 317 |
| PdmI | GAANNNNTTC | 3 cut(s) | 105, 108, 304 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 19 |
| PkrI | GCNGC | 2 cut(s) | 413, 475 |
| Ple19I | CGATCG | 1 cut(s) | 324 |
| PluTI | GGCGCC | 1 cut(s) | 229 |
| PspN4I | GGNNCC | 3 cut(s) | 140, 227, 307 |
| PspPI | GGNCC | 1 cut(s) | 252 |
| PvuI | CGATCG | 1 cut(s) | 324 |
| RsaI | GTAC | 2 cut(s) | 134, 494 |
| RsaNI | GTAC | 2 cut(s) | 133, 493 |
| Rsr2I | CGGWCCG | 1 cut(s) | 252 |
| RsrII | CGGWCCG | 1 cut(s) | 252 |
| SaqAI | TTAA | 2 cut(s) | 393, 482 |
| SatI | GCNGC | 2 cut(s) | 412, 474 |
| Sau3AI | GATC | 5 cut(s) | 145, 207, 321, 357, 402 |
| Sau96I | GGNCC | 1 cut(s) | 252 |
| ScrFI | CCNGG | 3 cut(s) | 212, 259, 449 |
| SduI | GDGCHC | 1 cut(s) | 317 |
| SetI | ASST | 4 cut(s) | 66, 307, 423, 488 |
| SfaNI | GCATC | 2 cut(s) | 362, 390 |
| SfoI | GGCGCC | 1 cut(s) | 227 |
| SinI | GGWCC | 1 cut(s) | 252 |
| Sse9I | AATT | 1 cut(s) | 104 |
| SsiI | CCGC | 5 cut(s) | 255, 318, 329, 346, 397 |
| SspDI | GGCGCC | 1 cut(s) | 225 |
| StyD4I | CCNGG | 3 cut(s) | 210, 257, 447 |
| StyI | CCWWGG | 1 cut(s) | 37 |
| TaaI | ACNGT | 1 cut(s) | 73 |
| TaiI | ACGT | 1 cut(s) | 66 |
| TaqI | TCGA | 6 cut(s) | 17, 77, 99, 114, 206, 370 |
| TasI | AATT | 1 cut(s) | 104 |
| Tru1I | TTAA | 2 cut(s) | 393, 482 |
| Tru9I | TTAA | 2 cut(s) | 393, 482 |
| TseI | GCWGC | 2 cut(s) | 411, 473 |
| TspDTI | ATGAA | 2 cut(s) | 17, 47 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 19 |
| VpaK11BI | GGWCC | 1 cut(s) | 252 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 481 |
| XapI | RAATTY | 1 cut(s) | 104 |
| XmnI | GAANNNNTTC | 3 cut(s) | 105, 108, 304 |