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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 230 |
| AciI | CCGC | 6 cut(s) | 149, 152, 180, 311, 350, 485 |
| AclI | AACGTT | 1 cut(s) | 340 |
| AcoI | YGGCCR | 1 cut(s) | 356 |
| AfaI | GTAC | 5 cut(s) | 68, 250, 271, 398, 531 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 176 |
| AgsI | TTSAA | 2 cut(s) | 44, 494 |
| AjnI | CCWGG | 1 cut(s) | 272 |
| AluBI | AGCT | 3 cut(s) | 85, 422, 490 |
| AluI | AGCT | 3 cut(s) | 85, 422, 490 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 216 |
| Ama87I | CYCGRG | 2 cut(s) | 223, 452 |
| AoxI | GGCC | 2 cut(s) | 54, 356 |
| ApeKI | GCWGC | 5 cut(s) | 104, 216, 261, 308, 487 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 33 |
| AspS9I | GGNCC | 1 cut(s) | 390 |
| AsuC2I | CCSGG | 5 cut(s) | 23, 224, 225, 253, 501 |
| AsuII | TTCGAA | 1 cut(s) | 189 |
| AvaI | CYCGRG | 2 cut(s) | 223, 452 |
| AvaII | GGWCC | 1 cut(s) | 390 |
| BanI | GGYRCC | 1 cut(s) | 230 |
| BanII | GRGCYC | 1 cut(s) | 77 |
| BbsI | GAAGAC | 1 cut(s) | 26 |
| BbvI | GCAGC | 5 cut(s) | 91, 203, 273, 295, 499 |
| BccI | CCATC | 1 cut(s) | 271 |
| BceAI | ACGGC | 3 cut(s) | 41, 104, 218 |
| BciT130I | CCWGG | 1 cut(s) | 274 |
| BcnI | CCSGG | 5 cut(s) | 23, 224, 225, 253, 501 |
| BfaI | CTAG | 1 cut(s) | 386 |
| BisI | GCNGC | 7 cut(s) | 105, 150, 217, 262, 309, 485, 488 |
| BlsI | GCNGC | 7 cut(s) | 106, 151, 218, 263, 310, 486, 489 |
| Bme1390I | CCNGG | 6 cut(s) | 23, 224, 225, 253, 274, 501 |
| Bme18I | GGWCC | 1 cut(s) | 390 |
| BmeT110I | CYCGRG | 2 cut(s) | 223, 452 |
| BmgT120I | GGNCC | 1 cut(s) | 390 |
| BmiI | GGNNCC | 3 cut(s) | 232, 240, 391 |
| BmrFI | CCNGG | 6 cut(s) | 23, 224, 225, 253, 274, 501 |
| BmsI | GCATC | 2 cut(s) | 228, 295 |
| BpiI | GAAGAC | 1 cut(s) | 26 |
| Bpu14I | TTCGAA | 1 cut(s) | 189 |
| BpuEI | CTTGAG | 1 cut(s) | 63 |
| BpuMI | CCSGG | 5 cut(s) | 23, 224, 225, 253, 501 |
| BsaJI | CCNNGG | 3 cut(s) | 223, 234, 499 |
| BsaWI | WCCGGW | 1 cut(s) | 520 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 219, 249 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 176 |
| Bse118I | RCCGGY | 1 cut(s) | 354 |
| Bse3DI | GCAATG | 2 cut(s) | 474, 479 |
| BseBI | CCWGG | 1 cut(s) | 274 |
| BseDI | CCNNGG | 3 cut(s) | 223, 234, 499 |
| BseGI | GGATG | 3 cut(s) | 168, 219, 282 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 176 |
| BseMI | GCAATG | 2 cut(s) | 474, 479 |
| BseRI | GAGGAG | 1 cut(s) | 324 |
| BseXI | GCAGC | 5 cut(s) | 91, 203, 273, 295, 499 |
| BshFI | GGCC | 2 cut(s) | 56, 358 |
| BshNI | GGYRCC | 1 cut(s) | 230 |
| BsiHKCI | CYCGRG | 2 cut(s) | 223, 452 |
| BsiSI | CCGG | 6 cut(s) | 23, 224, 252, 355, 501, 521 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 176 |
| BsnI | GGCC | 2 cut(s) | 56, 358 |
| BsoBI | CYCGRG | 2 cut(s) | 223, 452 |
| Bsp119I | TTCGAA | 1 cut(s) | 189 |
| Bsp1286I | GDGCHC | 1 cut(s) | 77 |
| BspACI | CCGC | 6 cut(s) | 149, 152, 180, 311, 350, 485 |
| BspANI | GGCC | 2 cut(s) | 56, 358 |
| BspHI | TCATGA | 1 cut(s) | 539 |
| BspLI | GGNNCC | 3 cut(s) | 232, 240, 391 |
| BspT104I | TTCGAA | 1 cut(s) | 189 |
| BspT107I | GGYRCC | 1 cut(s) | 230 |
| BsrDI | GCAATG | 2 cut(s) | 474, 479 |
| BsrFI | RCCGGY | 1 cut(s) | 354 |
| BssAI | RCCGGY | 1 cut(s) | 354 |
| BssECI | CCNNGG | 3 cut(s) | 223, 234, 499 |
| Bst2UI | CCWGG | 1 cut(s) | 274 |
| Bst4CI | ACNGT | 1 cut(s) | 462 |
| BstBI | TTCGAA | 1 cut(s) | 189 |
| BstDEI | CTNAG | 1 cut(s) | 129 |
| BstDSI | CCRYGG | 1 cut(s) | 234 |
| BstF5I | GGATG | 3 cut(s) | 168, 219, 282 |
| BstNI | CCWGG | 1 cut(s) | 274 |
| BstNSI | RCATGY | 1 cut(s) | 97 |
| BstSCI | CCNGG | 6 cut(s) | 21, 222, 223, 251, 272, 499 |
| BstV1I | GCAGC | 5 cut(s) | 91, 203, 273, 295, 499 |
| BstV2I | GAAGAC | 1 cut(s) | 26 |
| BsuRI | GGCC | 2 cut(s) | 56, 358 |
| BtgI | CCRYGG | 1 cut(s) | 234 |
| BtsCI | GGATG | 3 cut(s) | 168, 219, 282 |
| CaiI | CAGNNNCTG | 1 cut(s) | 216 |
| CciI | TCATGA | 1 cut(s) | 539 |
| Cfr10I | RCCGGY | 1 cut(s) | 354 |
| Cfr13I | GGNCC | 1 cut(s) | 390 |
| Cfr9I | CCCGGG | 1 cut(s) | 223 |
| Csp6I | GTAC | 5 cut(s) | 67, 249, 270, 397, 530 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 507, 542 |
| CviAII | CATG | 2 cut(s) | 94, 540 |
| CviQI | GTAC | 5 cut(s) | 67, 249, 270, 397, 530 |
| DdeI | CTNAG | 1 cut(s) | 129 |
| EaeI | YGGCCR | 1 cut(s) | 356 |
| EciI | GGCGGA | 1 cut(s) | 195 |
| Eco24I | GRGCYC | 1 cut(s) | 77 |
| Eco47I | GGWCC | 1 cut(s) | 390 |
| Eco88I | CYCGRG | 2 cut(s) | 223, 452 |
| EcoO109I | RGGNCCY | 1 cut(s) | 390 |
| EcoRII | CCWGG | 1 cut(s) | 272 |
| EcoT38I | GRGCYC | 1 cut(s) | 77 |
| FaeI | CATG | 2 cut(s) | 97, 543 |
| FaiI | YATR | 5 cut(s) | 95, 135, 158, 160, 541 |
| FatI | CATG | 2 cut(s) | 93, 539 |
| FauI | CCCGC | 2 cut(s) | 145, 304 |
| Fnu4HI | GCNGC | 7 cut(s) | 105, 150, 217, 262, 309, 485, 488 |
| FokI | GGATG | 3 cut(s) | 175, 206, 289 |
| FriOI | GRGCYC | 1 cut(s) | 77 |
| Fsp4HI | GCNGC | 7 cut(s) | 105, 150, 217, 262, 309, 485, 488 |
| FspBI | CTAG | 1 cut(s) | 386 |
| GluI | GCNGC | 7 cut(s) | 105, 150, 217, 262, 309, 485, 488 |
| HaeIII | GGCC | 2 cut(s) | 56, 358 |
| HapII | CCGG | 6 cut(s) | 23, 224, 252, 355, 501, 521 |
| Hin1II | CATG | 2 cut(s) | 97, 543 |
| HincII | GTYRAC | 1 cut(s) | 91 |
| HindII | GTYRAC | 1 cut(s) | 91 |
| HinfI | GANTC | 1 cut(s) | 371 |
| HpaII | CCGG | 6 cut(s) | 23, 224, 252, 355, 501, 521 |
| Hpy166II | GTNNAC | 1 cut(s) | 91 |
| Hpy188I | TCNGA | 1 cut(s) | 439 |
| Hpy188III | TCNNGA | 3 cut(s) | 80, 406, 540 |
| Hpy8I | GTNNAC | 1 cut(s) | 91 |
| HpyAV | CCTTC | 1 cut(s) | 405 |
| HpyCH4III | ACNGT | 1 cut(s) | 462 |
| HpyCH4IV | ACGT | 2 cut(s) | 31, 340 |
| HpyCH4V | TGCA | 2 cut(s) | 219, 479 |
| HpyF3I | CTNAG | 1 cut(s) | 129 |
| HpySE526I | ACGT | 2 cut(s) | 31, 340 |
| Hsp92II | CATG | 2 cut(s) | 97, 543 |
| Lsp1109I | GCAGC | 5 cut(s) | 91, 203, 273, 295, 499 |
| LweI | GCATC | 2 cut(s) | 228, 295 |
| MaeI | CTAG | 1 cut(s) | 386 |
| MaeII | ACGT | 2 cut(s) | 31, 340 |
| MaeIII | GTNAC | 1 cut(s) | 58 |
| MboII | GAAGA | 4 cut(s) | 26, 213, 355, 358 |
| MhlI | GDGCHC | 1 cut(s) | 77 |
| MlyI | GAGTC | 1 cut(s) | 380 |
| MnlI | CCTC | 8 cut(s) | 19, 46, 176, 206, 221, 342, 345, 403 |
| MroXI | GAANNNNTTC | 1 cut(s) | 33 |
| MspI | CCGG | 6 cut(s) | 23, 224, 252, 355, 501, 521 |
| MspR9I | CCNGG | 6 cut(s) | 23, 224, 225, 253, 274, 501 |
| MvaI | CCWGG | 1 cut(s) | 274 |
| NciI | CCSGG | 5 cut(s) | 23, 224, 225, 253, 501 |
| NlaIII | CATG | 2 cut(s) | 97, 543 |
| NlaIV | GGNNCC | 3 cut(s) | 232, 240, 391 |
| NspI | RCATGY | 1 cut(s) | 97 |
| NspV | TTCGAA | 1 cut(s) | 189 |
| PagI | TCATGA | 1 cut(s) | 539 |
| PdmI | GAANNNNTTC | 1 cut(s) | 33 |
| PkrI | GCNGC | 7 cut(s) | 106, 151, 218, 263, 310, 486, 489 |
| PleI | GAGTC | 1 cut(s) | 379 |
| PpsI | GAGTC | 1 cut(s) | 379 |
| PpuMI | RGGWCCY | 1 cut(s) | 390 |
| Psp1406I | AACGTT | 1 cut(s) | 340 |
| Psp5II | RGGWCCY | 1 cut(s) | 390 |
| Psp6I | CCWGG | 1 cut(s) | 272 |
| PspGI | CCWGG | 1 cut(s) | 272 |
| PspN4I | GGNNCC | 3 cut(s) | 232, 240, 391 |
| PspPI | GGNCC | 1 cut(s) | 390 |
| PspPPI | RGGWCCY | 1 cut(s) | 390 |
| PstNI | CAGNNNCTG | 1 cut(s) | 216 |
| RsaI | GTAC | 5 cut(s) | 68, 250, 271, 398, 531 |
| RsaNI | GTAC | 5 cut(s) | 67, 249, 270, 397, 530 |
| SatI | GCNGC | 7 cut(s) | 105, 150, 217, 262, 309, 485, 488 |
| Sau96I | GGNCC | 1 cut(s) | 390 |
| SchI | GAGTC | 1 cut(s) | 380 |
| ScrFI | CCNGG | 6 cut(s) | 23, 224, 225, 253, 274, 501 |
| SduI | GDGCHC | 1 cut(s) | 77 |
| SfaNI | GCATC | 2 cut(s) | 228, 295 |
| SfuI | TTCGAA | 1 cut(s) | 189 |
| SinI | GGWCC | 1 cut(s) | 390 |
| SmaI | CCCGGG | 1 cut(s) | 225 |
| SmlI | CTYRAG | 1 cut(s) | 78 |
| SmoI | CTYRAG | 1 cut(s) | 78 |
| SsiI | CCGC | 6 cut(s) | 149, 152, 180, 311, 350, 485 |
| SspMI | CTAG | 1 cut(s) | 386 |
| StyD4I | CCNGG | 6 cut(s) | 21, 222, 223, 251, 272, 499 |
| TaaI | ACNGT | 1 cut(s) | 462 |
| TaiI | ACGT | 2 cut(s) | 34, 343 |
| TaqI | TCGA | 2 cut(s) | 189, 369 |
| TatI | WGTACW | 1 cut(s) | 66 |
| TauI | GCSGC | 2 cut(s) | 152, 487 |
| TseI | GCWGC | 5 cut(s) | 104, 216, 261, 308, 487 |
| TspDTI | ATGAA | 2 cut(s) | 179, 525 |
| TspMI | CCCGGG | 1 cut(s) | 223 |
| VpaK11BI | GGWCC | 1 cut(s) | 390 |
| XceI | RCATGY | 1 cut(s) | 97 |
| XmaI | CCCGGG | 1 cut(s) | 223 |
| XmnI | GAANNNNTTC | 1 cut(s) | 33 |
| XspI | CTAG | 1 cut(s) | 386 |