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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 122 |
| AccB1I | GGYRCC | 2 cut(s) | 101, 122 |
| AciI | CCGC | 2 cut(s) | 54, 309 |
| AclWI | GGATC | 3 cut(s) | 205, 232, 245 |
| AcsI | RAATTY | 2 cut(s) | 218, 470 |
| AcuI | CTGAAG | 1 cut(s) | 315 |
| AfaI | GTAC | 3 cut(s) | 124, 307, 371 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 111, 247, 298, 299 |
| AflIII | ACRYGT | 1 cut(s) | 332 |
| AhlI | ACTAGT | 1 cut(s) | 17 |
| AjnI | CCWGG | 2 cut(s) | 184, 355 |
| AluBI | AGCT | 2 cut(s) | 119, 352 |
| AluI | AGCT | 2 cut(s) | 119, 352 |
| Alw26I | GTCTC | 1 cut(s) | 56 |
| AlwI | GGATC | 3 cut(s) | 205, 232, 245 |
| AoxI | GGCC | 1 cut(s) | 337 |
| ApeKI | GCWGC | 1 cut(s) | 56 |
| ApoI | RAATTY | 2 cut(s) | 218, 470 |
| Asp718I | GGTACC | 1 cut(s) | 122 |
| AspS9I | GGNCC | 1 cut(s) | 5 |
| AsuHPI | GGTGA | 4 cut(s) | 149, 281, 295, 538 |
| AsuNHI | GCTAGC | 1 cut(s) | 557 |
| AvaII | GGWCC | 1 cut(s) | 5 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 114, 147 |
| BamHI | GGATCC | 1 cut(s) | 237 |
| BanI | GGYRCC | 2 cut(s) | 101, 122 |
| BanII | GRGCYC | 1 cut(s) | 54 |
| BbsI | GAAGAC | 1 cut(s) | 585 |
| BbvI | GCAGC | 1 cut(s) | 43 |
| BccI | CCATC | 3 cut(s) | 158, 266, 274 |
| BceAI | ACGGC | 1 cut(s) | 296 |
| BciT130I | CCWGG | 2 cut(s) | 186, 357 |
| BcoDI | GTCTC | 1 cut(s) | 56 |
| BcuI | ACTAGT | 1 cut(s) | 17 |
| BfaI | CTAG | 2 cut(s) | 18, 558 |
| BfmI | CTRYAG | 1 cut(s) | 347 |
| BisI | GCNGC | 1 cut(s) | 57 |
| BlsI | GCNGC | 1 cut(s) | 58 |
| Bme1390I | CCNGG | 2 cut(s) | 186, 357 |
| Bme18I | GGWCC | 1 cut(s) | 5 |
| BmgT120I | GGNCC | 1 cut(s) | 5 |
| BmiI | GGNNCC | 6 cut(s) | 6, 7, 51, 103, 124, 239 |
| BmrFI | CCNGG | 2 cut(s) | 186, 357 |
| BmtI | GCTAGC | 1 cut(s) | 561 |
| BpiI | GAAGAC | 1 cut(s) | 585 |
| Bsa29I | ATCGAT | 1 cut(s) | 399 |
| BsaBI | GATNNNNATC | 1 cut(s) | 399 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 111, 247, 298, 299 |
| Bse8I | GATNNNNATC | 1 cut(s) | 399 |
| BseBI | CCWGG | 2 cut(s) | 186, 357 |
| BseCI | ATCGAT | 1 cut(s) | 399 |
| BseJI | GATNNNNATC | 1 cut(s) | 399 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 111, 247, 298, 299 |
| BseMII | CTCAG | 2 cut(s) | 51, 436 |
| BseRI | GAGGAG | 1 cut(s) | 62 |
| BseXI | GCAGC | 1 cut(s) | 43 |
| BshFI | GGCC | 1 cut(s) | 339 |
| BshNI | GGYRCC | 2 cut(s) | 101, 122 |
| BshVI | ATCGAT | 1 cut(s) | 399 |
| BslFI | GGGAC | 1 cut(s) | 181 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 111, 247, 298, 299 |
| BsmAI | GTCTC | 1 cut(s) | 56 |
| BsmFI | GGGAC | 1 cut(s) | 181 |
| BsnI | GGCC | 1 cut(s) | 339 |
| Bsp1286I | GDGCHC | 1 cut(s) | 54 |
| Bsp143I | GATC | 4 cut(s) | 210, 237, 400, 489 |
| BspACI | CCGC | 2 cut(s) | 54, 309 |
| BspANI | GGCC | 1 cut(s) | 339 |
| BspCNI | CTCAG | 2 cut(s) | 52, 435 |
| BspDI | ATCGAT | 1 cut(s) | 399 |
| BspHI | TCATGA | 1 cut(s) | 484 |
| BspLI | GGNNCC | 6 cut(s) | 6, 7, 51, 103, 124, 239 |
| BspOI | GCTAGC | 1 cut(s) | 561 |
| BspPI | GGATC | 3 cut(s) | 205, 232, 245 |
| BspT107I | GGYRCC | 2 cut(s) | 101, 122 |
| BssMI | GATC | 4 cut(s) | 210, 237, 400, 489 |
| Bst2UI | CCWGG | 2 cut(s) | 186, 357 |
| Bst4CI | ACNGT | 1 cut(s) | 442 |
| Bst6I | CTCTTC | 1 cut(s) | 291 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 265 |
| BstC8I | GCNNGC | 2 cut(s) | 54, 559 |
| BstDEI | CTNAG | 2 cut(s) | 60, 422 |
| BstKTI | GATC | 4 cut(s) | 213, 240, 403, 492 |
| BstMAI | GTCTC | 1 cut(s) | 56 |
| BstMBI | GATC | 4 cut(s) | 210, 237, 400, 489 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 65, 265 |
| BstNI | CCWGG | 2 cut(s) | 186, 357 |
| BstNSI | RCATGY | 1 cut(s) | 336 |
| BstSCI | CCNGG | 2 cut(s) | 184, 355 |
| BstSFI | CTRYAG | 1 cut(s) | 347 |
| BstV1I | GCAGC | 1 cut(s) | 43 |
| BstV2I | GAAGAC | 1 cut(s) | 585 |
| BstX2I | RGATCY | 2 cut(s) | 210, 237 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 133 |
| BstYI | RGATCY | 2 cut(s) | 210, 237 |
| Bsu15I | ATCGAT | 1 cut(s) | 399 |
| BsuRI | GGCC | 1 cut(s) | 339 |
| BsuTUI | ATCGAT | 1 cut(s) | 399 |
| Cac8I | GCNNGC | 2 cut(s) | 54, 559 |
| CciI | TCATGA | 1 cut(s) | 484 |
| Cfr13I | GGNCC | 1 cut(s) | 5 |
| ClaI | ATCGAT | 1 cut(s) | 399 |
| CsiI | ACCWGGT | 1 cut(s) | 355 |
| Csp6I | GTAC | 3 cut(s) | 123, 306, 370 |
| CviAII | CATG | 5 cut(s) | 133, 196, 333, 485, 550 |
| CviJI | RGCY | 6 cut(s) | 52, 119, 184, 339, 352, 557 |
| CviKI_1 | RGCY | 6 cut(s) | 52, 119, 184, 339, 352, 557 |
| CviQI | GTAC | 3 cut(s) | 123, 306, 370 |
| DdeI | CTNAG | 2 cut(s) | 60, 422 |
| DpnI | GATC | 4 cut(s) | 212, 239, 402, 491 |
| DpnII | GATC | 4 cut(s) | 210, 237, 400, 489 |
| Eam1104I | CTCTTC | 1 cut(s) | 291 |
| EarI | CTCTTC | 1 cut(s) | 291 |
| Eco24I | GRGCYC | 1 cut(s) | 54 |
| Eco32I | GATATC | 1 cut(s) | 397 |
| Eco47I | GGWCC | 1 cut(s) | 5 |
| Eco57I | CTGAAG | 1 cut(s) | 315 |
| EcoO109I | RGGNCCY | 1 cut(s) | 5 |
| EcoRII | CCWGG | 2 cut(s) | 184, 355 |
| EcoRV | GATATC | 1 cut(s) | 397 |
| EcoT38I | GRGCYC | 1 cut(s) | 54 |
| FaeI | CATG | 5 cut(s) | 136, 199, 336, 488, 553 |
| FaqI | GGGAC | 1 cut(s) | 181 |
| FatI | CATG | 5 cut(s) | 132, 195, 332, 484, 549 |
| FauI | CCCGC | 1 cut(s) | 61 |
| FauNDI | CATATG | 1 cut(s) | 244 |
| Fnu4HI | GCNGC | 1 cut(s) | 57 |
| FriOI | GRGCYC | 1 cut(s) | 54 |
| Fsp4HI | GCNGC | 1 cut(s) | 57 |
| FspBI | CTAG | 2 cut(s) | 18, 558 |
| GluI | GCNGC | 1 cut(s) | 57 |
| HaeIII | GGCC | 1 cut(s) | 339 |
| Hin1II | CATG | 5 cut(s) | 136, 199, 336, 488, 553 |
| HinfI | GANTC | 2 cut(s) | 169, 286 |
| HphI | GGTGA | 4 cut(s) | 149, 281, 295, 538 |
| Hpy188I | TCNGA | 1 cut(s) | 215 |
| Hpy188III | TCNNGA | 2 cut(s) | 392, 485 |
| HpyAV | CCTTC | 1 cut(s) | 547 |
| HpyCH4III | ACNGT | 1 cut(s) | 442 |
| HpyCH4V | TGCA | 3 cut(s) | 259, 367, 511 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 65, 265 |
| HpyF3I | CTNAG | 2 cut(s) | 60, 422 |
| Hsp92II | CATG | 5 cut(s) | 136, 199, 336, 488, 553 |
| KflI | GGGWCCC | 1 cut(s) | 5 |
| KpnI | GGTACC | 1 cut(s) | 126 |
| Kzo9I | GATC | 4 cut(s) | 210, 237, 400, 489 |
| LmnI | GCTCC | 1 cut(s) | 49 |
| Lsp1109I | GCAGC | 1 cut(s) | 43 |
| MabI | ACCWGGT | 1 cut(s) | 355 |
| MaeI | CTAG | 2 cut(s) | 18, 558 |
| MaeIII | GTNAC | 3 cut(s) | 287, 500, 544 |
| MalI | GATC | 4 cut(s) | 212, 239, 402, 491 |
| MboI | GATC | 4 cut(s) | 210, 237, 400, 489 |
| MboII | GAAGA | 4 cut(s) | 237, 308, 535, 585 |
| MfeI | CAATTG | 1 cut(s) | 428 |
| MflI | RGATCY | 2 cut(s) | 210, 237 |
| MhlI | GDGCHC | 1 cut(s) | 54 |
| MluCI | AATT | 4 cut(s) | 218, 428, 470, 506 |
| MlyI | GAGTC | 2 cut(s) | 163, 295 |
| MnlI | CCTC | 8 cut(s) | 33, 40, 105, 196, 228, 241, 292, 574 |
| MseI | TTAA | 1 cut(s) | 387 |
| MslI | CAYNNNNRTG | 1 cut(s) | 131 |
| MspA1I | CMGCKG | 2 cut(s) | 56, 119 |
| MspR9I | CCNGG | 2 cut(s) | 186, 357 |
| MunI | CAATTG | 1 cut(s) | 428 |
| MvaI | CCWGG | 2 cut(s) | 186, 357 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 65, 265 |
| NdeI | CATATG | 1 cut(s) | 244 |
| NdeII | GATC | 4 cut(s) | 210, 237, 400, 489 |
| NheI | GCTAGC | 1 cut(s) | 557 |
| NlaIII | CATG | 5 cut(s) | 136, 199, 336, 488, 553 |
| NlaIV | GGNNCC | 6 cut(s) | 6, 7, 51, 103, 124, 239 |
| NmuCI | GTSAC | 2 cut(s) | 287, 544 |
| NspI | RCATGY | 1 cut(s) | 336 |
| PagI | TCATGA | 1 cut(s) | 484 |
| PciI | ACATGT | 1 cut(s) | 332 |
| PkrI | GCNGC | 1 cut(s) | 58 |
| PleI | GAGTC | 2 cut(s) | 163, 294 |
| PpsI | GAGTC | 2 cut(s) | 163, 294 |
| PpuMI | RGGWCCY | 1 cut(s) | 5 |
| PscI | ACATGT | 1 cut(s) | 332 |
| Psp5II | RGGWCCY | 1 cut(s) | 5 |
| Psp6I | CCWGG | 2 cut(s) | 184, 355 |
| PspGI | CCWGG | 2 cut(s) | 184, 355 |
| PspN4I | GGNNCC | 6 cut(s) | 6, 7, 51, 103, 124, 239 |
| PspPI | GGNCC | 1 cut(s) | 5 |
| PspPPI | RGGWCCY | 1 cut(s) | 5 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 362, 394 |
| PsuI | RGATCY | 2 cut(s) | 210, 237 |
| PvuII | CAGCTG | 1 cut(s) | 119 |
| RsaI | GTAC | 3 cut(s) | 124, 307, 371 |
| RsaNI | GTAC | 3 cut(s) | 123, 306, 370 |
| RseI | CAYNNNNRTG | 1 cut(s) | 131 |
| SaqAI | TTAA | 1 cut(s) | 387 |
| SatI | GCNGC | 1 cut(s) | 57 |
| Sau3AI | GATC | 4 cut(s) | 210, 237, 400, 489 |
| Sau96I | GGNCC | 1 cut(s) | 5 |
| SchI | GAGTC | 2 cut(s) | 163, 295 |
| ScrFI | CCNGG | 2 cut(s) | 186, 357 |
| SduI | GDGCHC | 1 cut(s) | 54 |
| SetI | ASST | 8 cut(s) | 116, 121, 252, 265, 354, 361, 387, 408 |
| SexAI | ACCWGGT | 1 cut(s) | 355 |
| SfcI | CTRYAG | 1 cut(s) | 347 |
| SinI | GGWCC | 1 cut(s) | 5 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 131 |
| SpeI | ACTAGT | 1 cut(s) | 17 |
| Sse9I | AATT | 4 cut(s) | 218, 428, 470, 506 |
| SsiI | CCGC | 2 cut(s) | 54, 309 |
| SspMI | CTAG | 2 cut(s) | 18, 558 |
| StyD4I | CCNGG | 2 cut(s) | 184, 355 |
| TaaI | ACNGT | 1 cut(s) | 442 |
| TaqI | TCGA | 2 cut(s) | 393, 399 |
| TasI | AATT | 4 cut(s) | 218, 428, 470, 506 |
| TatI | WGTACW | 1 cut(s) | 369 |
| Tru1I | TTAA | 1 cut(s) | 387 |
| Tru9I | TTAA | 1 cut(s) | 387 |
| TseFI | GTSAC | 2 cut(s) | 287, 544 |
| TseI | GCWGC | 1 cut(s) | 56 |
| Tsp45I | GTSAC | 2 cut(s) | 287, 544 |
| TspDTI | ATGAA | 1 cut(s) | 566 |
| TspGWI | ACGGA | 1 cut(s) | 586 |
| VpaK11BI | GGWCC | 1 cut(s) | 5 |
| XapI | RAATTY | 2 cut(s) | 218, 470 |
| XceI | RCATGY | 1 cut(s) | 336 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 76, 332 |
| XspI | CTAG | 2 cut(s) | 18, 558 |