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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1771 |
| AatII | GACGTC | 1 cut(s) | 1152 |
| Acc36I | ACCTGC | 1 cut(s) | 1771 |
| Acc65I | GGTACC | 3 cut(s) | 195, 881, 1448 |
| AccB1I | GGYRCC | 4 cut(s) | 195, 881, 1448, 2192 |
| AccI | GTMKAC | 3 cut(s) | 210, 420, 568 |
| AccII | CGCG | 5 cut(s) | 103, 491, 699, 701, 1900 |
| AclWI | GGATC | 8 cut(s) | 5, 304, 1226, 1380, 1492, 1620, 1995, 2229 |
| AcoI | YGGCCR | 5 cut(s) | 324, 486, 1272, 1432, 1848 |
| AcsI | RAATTY | 2 cut(s) | 64, 2132 |
| AcuI | CTGAAG | 4 cut(s) | 198, 452, 1637, 1772 |
| AcyI | GRCGYC | 3 cut(s) | 1149, 1402, 1728 |
| AdeI | CACNNNGTG | 1 cut(s) | 1258 |
| AgeI | ACCGGT | 2 cut(s) | 192, 1451 |
| AgsI | TTSAA | 3 cut(s) | 64, 890, 1187 |
| AjnI | CCWGG | 5 cut(s) | 388, 1122, 1443, 1662, 2148 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 218, 250 |
| Alw21I | GWGCWC | 1 cut(s) | 983 |
| Alw26I | GTCTC | 7 cut(s) | 116, 140, 1184, 1682, 1960, 2150, 2208 |
| AlwI | GGATC | 8 cut(s) | 5, 304, 1226, 1380, 1492, 1620, 1995, 2229 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 577, 1561 |
| Ama87I | CYCGRG | 7 cut(s) | 117, 332, 502, 945, 1480, 1683, 1920 |
| ApoI | RAATTY | 2 cut(s) | 64, 2132 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1560, 1592 |
| AsiGI | ACCGGT | 2 cut(s) | 192, 1451 |
| Asp718I | GGTACC | 3 cut(s) | 195, 881, 1448 |
| AspA2I | CCTAGG | 1 cut(s) | 199 |
| AspLEI | GCGC | 4 cut(s) | 517, 701, 1363, 1900 |
| AspS9I | GGNCC | 8 cut(s) | 43, 234, 372, 658, 901, 1249, 1350, 1490 |
| AsuHPI | GGTGA | 9 cut(s) | 293, 591, 760, 1235, 1385, 1432, 1469, 1488, 1795 |
| AvaI | CYCGRG | 7 cut(s) | 117, 332, 502, 945, 1480, 1683, 1920 |
| AvaII | GGWCC | 6 cut(s) | 43, 234, 372, 658, 901, 1350 |
| AvrII | CCTAGG | 1 cut(s) | 199 |
| BaeGI | GKGCMC | 1 cut(s) | 1705 |
| BalI | TGGCCA | 1 cut(s) | 326 |
| BanI | GGYRCC | 4 cut(s) | 195, 881, 1448, 2192 |
| BanII | GRGCYC | 1 cut(s) | 983 |
| BauI | CACGAG | 3 cut(s) | 521, 1171, 2009 |
| BbsI | GAAGAC | 1 cut(s) | 1086 |
| Bbv12I | GWGCWC | 1 cut(s) | 983 |
| BccI | CCATC | 9 cut(s) | 470, 478, 640, 1081, 1099, 1556, 1730, 1840, 2210 |
| BceAI | ACGGC | 6 cut(s) | 33, 608, 941, 1477, 1508, 1937 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 340, 374 |
| BciT130I | CCWGG | 5 cut(s) | 390, 1124, 1445, 1664, 2150 |
| BciVI | GTATCC | 3 cut(s) | 435, 1211, 1735 |
| BcoDI | GTCTC | 7 cut(s) | 116, 140, 1184, 1682, 1960, 2150, 2208 |
| BfaI | CTAG | 7 cut(s) | 200, 762, 779, 833, 1019, 1157, 2120 |
| BfmI | CTRYAG | 2 cut(s) | 569, 1941 |
| BfoI | RGCGCY | 2 cut(s) | 518, 1364 |
| BfuAI | ACCTGC | 1 cut(s) | 1771 |
| BfuI | GTATCC | 3 cut(s) | 435, 1211, 1735 |
| BlnI | CCTAGG | 1 cut(s) | 199 |
| Bme18I | GGWCC | 6 cut(s) | 43, 234, 372, 658, 901, 1350 |
| BmeT110I | CYCGRG | 7 cut(s) | 117, 332, 502, 945, 1480, 1683, 1920 |
| BmgT120I | GGNCC | 8 cut(s) | 43, 234, 372, 658, 901, 1249, 1350, 1490 |
| BmrI | ACTGGG | 2 cut(s) | 252, 1356 |
| BmsI | GCATC | 4 cut(s) | 39, 576, 1123, 1686 |
| BmuI | ACTGGG | 2 cut(s) | 252, 1356 |
| BpiI | GAAGAC | 1 cut(s) | 1086 |
| BplI | GAGNNNNNCTC | 6 cut(s) | 965, 997, 1814, 1846, 2033, 2065 |
| BpmI | CTGGAG | 4 cut(s) | 1835, 2003, 2171, 2192 |
| Bpu10I | CCTNAGC | 2 cut(s) | 345, 461 |
| BpuEI | CTTGAG | 2 cut(s) | 706, 1898 |
| Bsa29I | ATCGAT | 1 cut(s) | 1691 |
| BsaBI | GATNNNNATC | 2 cut(s) | 1746, 2220 |
| BsaHI | GRCGYC | 3 cut(s) | 1149, 1402, 1728 |
| BsaI | GGTCTC | 3 cut(s) | 1184, 2150, 2208 |
| BsaWI | WCCGGW | 6 cut(s) | 192, 310, 445, 1298, 1451, 1528 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1409, 1439, 2015, 2045 |
| Bse118I | RCCGGY | 5 cut(s) | 192, 1315, 1398, 1451, 2195 |
| Bse1I | ACTGG | 5 cut(s) | 247, 872, 904, 1351, 1435 |
| Bse8I | GATNNNNATC | 2 cut(s) | 1746, 2220 |
| BseBI | CCWGG | 5 cut(s) | 390, 1124, 1445, 1664, 2150 |
| BseCI | ATCGAT | 1 cut(s) | 1691 |
| BseGI | GGATG | 6 cut(s) | 481, 591, 993, 1092, 1096, 1444 |
| BseJI | GATNNNNATC | 2 cut(s) | 1746, 2220 |
| BseMII | CTCAG | 4 cut(s) | 1833, 1884, 2049, 2219 |
| BseNI | ACTGG | 5 cut(s) | 247, 872, 904, 1351, 1435 |
| BseRI | GAGGAG | 5 cut(s) | 991, 1751, 1772, 1919, 2036 |
| BseSI | GKGCMC | 1 cut(s) | 1705 |
| BsgI | GTGCAG | 3 cut(s) | 503, 1124, 1280 |
| Bsh1236I | CGCG | 5 cut(s) | 103, 491, 699, 701, 1900 |
| Bsh1285I | CGRYCG | 2 cut(s) | 193, 1636 |
| BshNI | GGYRCC | 4 cut(s) | 195, 881, 1448, 2192 |
| BshTI | ACCGGT | 2 cut(s) | 192, 1451 |
| BshVI | ATCGAT | 1 cut(s) | 1691 |
| BsiEI | CGRYCG | 2 cut(s) | 193, 1636 |
| BsiHKAI | GWGCWC | 1 cut(s) | 983 |
| BsiHKCI | CYCGRG | 7 cut(s) | 117, 332, 502, 945, 1480, 1683, 1920 |
| BslFI | GGGAC | 5 cut(s) | 385, 671, 914, 1363, 1937 |
| BsmAI | GTCTC | 7 cut(s) | 116, 140, 1184, 1682, 1960, 2150, 2208 |
| BsmBI | CGTCTC | 1 cut(s) | 1960 |
| BsmFI | GGGAC | 5 cut(s) | 385, 671, 914, 1363, 1937 |
| BsmI | GAATGC | 3 cut(s) | 339, 625, 1921 |
| Bso31I | GGTCTC | 3 cut(s) | 1184, 2150, 2208 |
| BsoBI | CYCGRG | 7 cut(s) | 117, 332, 502, 945, 1480, 1683, 1920 |
| Bsp1286I | GDGCHC | 2 cut(s) | 983, 1705 |
| Bsp19I | CCATGG | 1 cut(s) | 1269 |
| Bsp68I | TCGCGA | 1 cut(s) | 103 |
| BspCNI | CTCAG | 4 cut(s) | 1834, 1885, 2048, 2218 |
| BspDI | ATCGAT | 1 cut(s) | 1691 |
| BspFNI | CGCG | 5 cut(s) | 103, 491, 699, 701, 1900 |
| BspHI | TCATGA | 1 cut(s) | 2085 |
| BspMI | ACCTGC | 1 cut(s) | 1771 |
| BspPI | GGATC | 8 cut(s) | 5, 304, 1226, 1380, 1492, 1620, 1995, 2229 |
| BspT107I | GGYRCC | 4 cut(s) | 195, 881, 1448, 2192 |
| BspTNI | GGTCTC | 3 cut(s) | 1184, 2150, 2208 |
| BsrFI | RCCGGY | 5 cut(s) | 192, 1315, 1398, 1451, 2195 |
| BsrI | ACTGG | 5 cut(s) | 247, 872, 904, 1351, 1435 |
| BssAI | RCCGGY | 5 cut(s) | 192, 1315, 1398, 1451, 2195 |
| BssNI | GRCGYC | 3 cut(s) | 1149, 1402, 1728 |
| BssSI | CACGAG | 3 cut(s) | 521, 1171, 2009 |
| BssT1I | CCWWGG | 5 cut(s) | 159, 199, 538, 1269, 1504 |
| Bst2BI | CACGAG | 3 cut(s) | 521, 1171, 2009 |
| Bst2UI | CCWGG | 5 cut(s) | 390, 1124, 1445, 1664, 2150 |
| Bst4CI | ACNGT | 6 cut(s) | 43, 85, 322, 573, 748, 1223 |
| Bst6I | CTCTTC | 6 cut(s) | 929, 1046, 1182, 1339, 1376, 1454 |
| BstACI | GRCGYC | 3 cut(s) | 1149, 1402, 1728 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1603 |
| BstC8I | GCNNGC | 7 cut(s) | 174, 668, 1701, 1732, 2058, 2182, 2197 |
| BstDEI | CTNAG | 7 cut(s) | 345, 461, 470, 1842, 1893, 2035, 2205 |
| BstDSI | CCRYGG | 2 cut(s) | 1269, 1998 |
| BstEII | GGTNACC | 1 cut(s) | 748 |
| BstF5I | GGATG | 6 cut(s) | 481, 591, 993, 1092, 1096, 1444 |
| BstFNI | CGCG | 5 cut(s) | 103, 491, 699, 701, 1900 |
| BstH2I | RGCGCY | 2 cut(s) | 518, 1364 |
| BstHHI | GCGC | 4 cut(s) | 517, 701, 1363, 1900 |
| BstMAI | GTCTC | 7 cut(s) | 116, 140, 1184, 1682, 1960, 2150, 2208 |
| BstMCI | CGRYCG | 2 cut(s) | 193, 1636 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 497, 586, 676, 1247, 1603, 1727, 1847 |
| BstNI | CCWGG | 5 cut(s) | 390, 1124, 1445, 1664, 2150 |
| BstNSI | RCATGY | 3 cut(s) | 361, 427, 1114 |
| BstPI | GGTNACC | 1 cut(s) | 748 |
| BstSFI | CTRYAG | 2 cut(s) | 569, 1941 |
| BstSLI | GKGCMC | 1 cut(s) | 1705 |
| BstUI | CGCG | 5 cut(s) | 103, 491, 699, 701, 1900 |
| BstV2I | GAAGAC | 1 cut(s) | 1086 |
| BstX2I | RGATCY | 3 cut(s) | 296, 1385, 1612 |
| BstYI | RGATCY | 3 cut(s) | 296, 1385, 1612 |
| Bsu15I | ATCGAT | 1 cut(s) | 1691 |
| BsuI | GTATCC | 3 cut(s) | 435, 1211, 1735 |
| BsuTUI | ATCGAT | 1 cut(s) | 1691 |
| BtgI | CCRYGG | 2 cut(s) | 1269, 1998 |
| BtsCI | GGATG | 6 cut(s) | 481, 591, 993, 1092, 1096, 1444 |
| BtsIMutI | CAGTG | 2 cut(s) | 327, 1428 |
| BtuMI | TCGCGA | 1 cut(s) | 103 |
| BveI | ACCTGC | 1 cut(s) | 1771 |
| Cac8I | GCNNGC | 7 cut(s) | 174, 668, 1701, 1732, 2058, 2182, 2197 |
| CaiI | CAGNNNCTG | 2 cut(s) | 577, 1561 |
| CciI | TCATGA | 1 cut(s) | 2085 |
| CfoI | GCGC | 4 cut(s) | 517, 701, 1363, 1900 |
| Cfr10I | RCCGGY | 5 cut(s) | 192, 1315, 1398, 1451, 2195 |
| Cfr13I | GGNCC | 8 cut(s) | 43, 234, 372, 658, 901, 1249, 1350, 1490 |
| Cfr9I | CCCGGG | 4 cut(s) | 502, 945, 1480, 1920 |
| ClaI | ATCGAT | 1 cut(s) | 1691 |
| CseI | GACGC | 4 cut(s) | 1103, 1247, 1391, 1736 |
| CspAI | ACCGGT | 2 cut(s) | 192, 1451 |
| DdeI | CTNAG | 7 cut(s) | 345, 461, 470, 1842, 1893, 2035, 2205 |
| DraIII | CACNNNGTG | 1 cut(s) | 1258 |
| EaeI | YGGCCR | 5 cut(s) | 324, 486, 1272, 1432, 1848 |
| Eam1104I | CTCTTC | 6 cut(s) | 929, 1046, 1182, 1339, 1376, 1454 |
| EarI | CTCTTC | 6 cut(s) | 929, 1046, 1182, 1339, 1376, 1454 |
| Ecl136II | GAGCTC | 1 cut(s) | 981 |
| Eco130I | CCWWGG | 5 cut(s) | 159, 199, 538, 1269, 1504 |
| Eco24I | GRGCYC | 1 cut(s) | 983 |
| Eco31I | GGTCTC | 3 cut(s) | 1184, 2150, 2208 |
| Eco32I | GATATC | 1 cut(s) | 1624 |
| Eco47I | GGWCC | 6 cut(s) | 43, 234, 372, 658, 901, 1350 |
| Eco53kI | GAGCTC | 1 cut(s) | 981 |
| Eco57I | CTGAAG | 4 cut(s) | 198, 452, 1637, 1772 |
| Eco88I | CYCGRG | 7 cut(s) | 117, 332, 502, 945, 1480, 1683, 1920 |
| Eco91I | GGTNACC | 1 cut(s) | 748 |
| EcoICRI | GAGCTC | 1 cut(s) | 981 |
| EcoO109I | RGGNCCY | 2 cut(s) | 234, 658 |
| EcoO65I | GGTNACC | 1 cut(s) | 748 |
| EcoRII | CCWGG | 5 cut(s) | 388, 1122, 1443, 1662, 2148 |
| EcoRV | GATATC | 1 cut(s) | 1624 |
| EcoT14I | CCWWGG | 5 cut(s) | 159, 199, 538, 1269, 1504 |
| EcoT38I | GRGCYC | 1 cut(s) | 983 |
| ErhI | CCWWGG | 5 cut(s) | 159, 199, 538, 1269, 1504 |
| Esp3I | CGTCTC | 1 cut(s) | 1960 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1179, 1211 |
| FaqI | GGGAC | 5 cut(s) | 385, 671, 914, 1363, 1937 |
| FauI | CCCGC | 7 cut(s) | 733, 1528, 1692, 1893, 2133, 2173, 2192 |
| FauNDI | CATATG | 1 cut(s) | 377 |
| FblI | GTMKAC | 3 cut(s) | 210, 420, 568 |
| FokI | GGATG | 6 cut(s) | 488, 598, 980, 1099, 1103, 1451 |
| FriOI | GRGCYC | 1 cut(s) | 983 |
| FspBI | CTAG | 7 cut(s) | 200, 762, 779, 833, 1019, 1157, 2120 |
| GlaI | GCGC | 4 cut(s) | 516, 700, 1362, 1899 |
| GsuI | CTGGAG | 4 cut(s) | 1835, 2003, 2171, 2192 |
| HaeII | RGCGCY | 2 cut(s) | 518, 1364 |
| HgaI | GACGC | 4 cut(s) | 1103, 1247, 1391, 1736 |
| HhaI | GCGC | 4 cut(s) | 517, 701, 1363, 1900 |
| Hin1I | GRCGYC | 3 cut(s) | 1149, 1402, 1728 |
| Hin6I | GCGC | 4 cut(s) | 515, 699, 1361, 1898 |
| HinP1I | GCGC | 4 cut(s) | 515, 699, 1361, 1898 |
| HphI | GGTGA | 9 cut(s) | 293, 591, 760, 1235, 1385, 1432, 1469, 1488, 1795 |
| Hpy166II | GTNNAC | 8 cut(s) | 184, 211, 421, 569, 706, 1226, 1428, 1717 |
| Hpy188I | TCNGA | 9 cut(s) | 256, 274, 922, 967, 1617, 1752, 1930, 2038, 2208 |
| Hpy188III | TCNNGA | 8 cut(s) | 46, 102, 119, 332, 1235, 1982, 2086, 2120 |
| Hpy8I | GTNNAC | 8 cut(s) | 184, 211, 421, 569, 706, 1226, 1428, 1717 |
| Hpy99I | CGWCG | 7 cut(s) | 43, 278, 386, 1151, 1154, 1241, 1730 |
| HpyCH4III | ACNGT | 6 cut(s) | 43, 85, 322, 573, 748, 1223 |
| HpyCH4IV | ACGT | 2 cut(s) | 1149, 1424 |
| HpyCH4V | TGCA | 6 cut(s) | 138, 172, 520, 1141, 1261, 1597 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 497, 586, 676, 1247, 1603, 1727, 1847 |
| HpyF3I | CTNAG | 7 cut(s) | 345, 461, 470, 1842, 1893, 2035, 2205 |
| HpySE526I | ACGT | 2 cut(s) | 1149, 1424 |
| Hsp92I | GRCGYC | 3 cut(s) | 1149, 1402, 1728 |
| HspAI | GCGC | 4 cut(s) | 515, 699, 1361, 1898 |
| KpnI | GGTACC | 3 cut(s) | 199, 885, 1452 |
| KroI | GCCGGC | 1 cut(s) | 2195 |
| KroNI | GCCGGC | 1 cut(s) | 2197 |
| LmnI | GCTCC | 7 cut(s) | 313, 537, 978, 1038, 1246, 1822, 1873 |
| LweI | GCATC | 4 cut(s) | 39, 576, 1123, 1686 |
| MaeI | CTAG | 7 cut(s) | 200, 762, 779, 833, 1019, 1157, 2120 |
| MaeII | ACGT | 2 cut(s) | 1149, 1424 |
| MaeIII | GTNAC | 7 cut(s) | 316, 597, 748, 781, 1420, 1494, 1888 |
| MflI | RGATCY | 3 cut(s) | 296, 1385, 1612 |
| MhlI | GDGCHC | 2 cut(s) | 983, 1705 |
| MlsI | TGGCCA | 1 cut(s) | 326 |
| MluCI | AATT | 4 cut(s) | 64, 167, 215, 2132 |
| MluNI | TGGCCA | 1 cut(s) | 326 |
| MlyI | GAGTC | 6 cut(s) | 278, 1183, 1823, 1919, 1955, 1970 |
| MmeI | TCCRAC | 3 cut(s) | 35, 297, 945 |
| Mox20I | TGGCCA | 1 cut(s) | 326 |
| MroNI | GCCGGC | 1 cut(s) | 2195 |
| MscI | TGGCCA | 1 cut(s) | 326 |
| MseI | TTAA | 3 cut(s) | 68, 1710, 2094 |
| MslI | CAYNNNNRTG | 3 cut(s) | 404, 1115, 1301 |
| Msp20I | TGGCCA | 1 cut(s) | 326 |
| MspA1I | CMGCKG | 4 cut(s) | 610, 874, 1558, 1864 |
| Mva1269I | GAATGC | 3 cut(s) | 339, 625, 1921 |
| MvaI | CCWGG | 5 cut(s) | 390, 1124, 1445, 1664, 2150 |
| MvnI | CGCG | 5 cut(s) | 103, 491, 699, 701, 1900 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 497, 586, 676, 1247, 1603, 1727, 1847 |
| NaeI | GCCGGC | 1 cut(s) | 2197 |
| NcoI | CCATGG | 1 cut(s) | 1269 |
| NdeI | CATATG | 1 cut(s) | 377 |
| NgoMIV | GCCGGC | 1 cut(s) | 2195 |
| NmeAIII | GCCGAG | 1 cut(s) | 375 |
| NmuCI | GTSAC | 5 cut(s) | 316, 597, 748, 1420, 1494 |
| NruI | TCGCGA | 1 cut(s) | 103 |
| NspI | RCATGY | 3 cut(s) | 361, 427, 1114 |
| PaeR7I | CTCGAG | 1 cut(s) | 117 |
| PagI | TCATGA | 1 cut(s) | 2085 |
| PaqCI | CACCTGC | 1 cut(s) | 1771 |
| PcsI | WCGNNNNNNNCGW | 4 cut(s) | 236, 1169, 1578, 1731 |
| PctI | GAATGC | 3 cut(s) | 339, 625, 1921 |
| PdiI | GCCGGC | 1 cut(s) | 2197 |
| PfeI | GAWTC | 5 cut(s) | 493, 526, 990, 1669, 1688 |
| PfoI | TCCNGGA | 1 cut(s) | 2148 |
| PinAI | ACCGGT | 2 cut(s) | 192, 1451 |
| PleI | GAGTC | 6 cut(s) | 277, 1182, 1823, 1919, 1954, 1970 |
| PpsI | GAGTC | 6 cut(s) | 277, 1182, 1823, 1919, 1954, 1970 |
| PpuMI | RGGWCCY | 2 cut(s) | 234, 658 |
| Psp124BI | GAGCTC | 1 cut(s) | 983 |
| Psp5II | RGGWCCY | 2 cut(s) | 234, 658 |
| Psp6I | CCWGG | 5 cut(s) | 388, 1122, 1443, 1662, 2148 |
| PspEI | GGTNACC | 1 cut(s) | 748 |
| PspGI | CCWGG | 5 cut(s) | 388, 1122, 1443, 1662, 2148 |
| PspPI | GGNCC | 8 cut(s) | 43, 234, 372, 658, 901, 1249, 1350, 1490 |
| PspPPI | RGGWCCY | 2 cut(s) | 234, 658 |
| PstNI | CAGNNNCTG | 2 cut(s) | 577, 1561 |
| PsuI | RGATCY | 3 cut(s) | 296, 1385, 1612 |
| PvuII | CAGCTG | 3 cut(s) | 610, 874, 1558 |
| RruI | TCGCGA | 1 cut(s) | 103 |
| RseI | CAYNNNNRTG | 3 cut(s) | 404, 1115, 1301 |
| SacI | GAGCTC | 1 cut(s) | 983 |
| SaqAI | TTAA | 3 cut(s) | 68, 1710, 2094 |
| Sau96I | GGNCC | 8 cut(s) | 43, 234, 372, 658, 901, 1249, 1350, 1490 |
| SchI | GAGTC | 6 cut(s) | 278, 1183, 1823, 1919, 1955, 1970 |
| SduI | GDGCHC | 2 cut(s) | 983, 1705 |
| SfaNI | GCATC | 4 cut(s) | 39, 576, 1123, 1686 |
| SfcI | CTRYAG | 2 cut(s) | 569, 1941 |
| Sfr274I | CTCGAG | 1 cut(s) | 117 |
| SgrAI | CRCCGGYG | 1 cut(s) | 1398 |
| SinI | GGWCC | 6 cut(s) | 43, 234, 372, 658, 901, 1350 |
| SlaI | CTCGAG | 1 cut(s) | 117 |
| SmaI | CCCGGG | 4 cut(s) | 504, 947, 1482, 1922 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 404, 1115, 1301 |
| SmlI | CTYRAG | 3 cut(s) | 117, 721, 1877 |
| SmoI | CTYRAG | 3 cut(s) | 117, 721, 1877 |
| Sse9I | AATT | 4 cut(s) | 64, 167, 215, 2132 |
| SspMI | CTAG | 7 cut(s) | 200, 762, 779, 833, 1019, 1157, 2120 |
| SstI | GAGCTC | 1 cut(s) | 983 |
| StyI | CCWWGG | 5 cut(s) | 159, 199, 538, 1269, 1504 |
| TaaI | ACNGT | 6 cut(s) | 43, 85, 322, 573, 748, 1223 |
| TaiI | ACGT | 2 cut(s) | 1152, 1427 |
| TaqII | GACCGA | 1 cut(s) | 1367 |
| TasI | AATT | 4 cut(s) | 64, 167, 215, 2132 |
| TatI | WGTACW | 2 cut(s) | 717, 2025 |
| TauI | GCSGC | 6 cut(s) | 430, 491, 761, 778, 1853, 2180 |
| TfiI | GAWTC | 5 cut(s) | 493, 526, 990, 1669, 1688 |
| Tru1I | TTAA | 3 cut(s) | 68, 1710, 2094 |
| Tru9I | TTAA | 3 cut(s) | 68, 1710, 2094 |
| TscAI | CASTG | 2 cut(s) | 327, 1435 |
| TseFI | GTSAC | 5 cut(s) | 316, 597, 748, 1420, 1494 |
| Tsp45I | GTSAC | 5 cut(s) | 316, 597, 748, 1420, 1494 |
| TspDTI | ATGAA | 6 cut(s) | 422, 982, 1582, 1599, 1784, 2102 |
| TspGWI | ACGGA | 2 cut(s) | 380, 2015 |
| TspMI | CCCGGG | 4 cut(s) | 502, 945, 1480, 1920 |
| TspRI | CASTG | 2 cut(s) | 327, 1435 |
| VpaK11BI | GGWCC | 6 cut(s) | 43, 234, 372, 658, 901, 1350 |
| XapI | RAATTY | 2 cut(s) | 64, 2132 |
| XbaI | TCTAGA | 1 cut(s) | 2119 |
| XceI | RCATGY | 3 cut(s) | 361, 427, 1114 |
| XhoI | CTCGAG | 1 cut(s) | 117 |
| XmaI | CCCGGG | 4 cut(s) | 502, 945, 1480, 1920 |
| XmaJI | CCTAGG | 1 cut(s) | 199 |
| XmiI | GTMKAC | 3 cut(s) | 210, 420, 568 |
| XspI | CTAG | 7 cut(s) | 200, 762, 779, 833, 1019, 1157, 2120 |
| ZraI | GACGTC | 1 cut(s) | 1150 |