No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 3 cut(s) | 308, 338, 377 |
| AciI | CCGC | 4 cut(s) | 188, 251, 338, 375 |
| AclWI | GGATC | 4 cut(s) | 40, 114, 127, 190 |
| AcuI | CTGAAG | 1 cut(s) | 84 |
| AfaI | GTAC | 2 cut(s) | 41, 83 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 21, 129, 393, 394 |
| AjnI | CCWGG | 2 cut(s) | 14, 53 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 104, 136 |
| AluBI | AGCT | 3 cut(s) | 62, 346, 370 |
| AluI | AGCT | 3 cut(s) | 62, 346, 370 |
| AlwI | GGATC | 4 cut(s) | 40, 114, 127, 190 |
| Ama87I | CYCGRG | 3 cut(s) | 218, 275, 388 |
| AoxI | GGCC | 2 cut(s) | 192, 350 |
| ApeKI | GCWGC | 1 cut(s) | 403 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 416 |
| AspA2I | CCTAGG | 1 cut(s) | 236 |
| AspLEI | GCGC | 1 cut(s) | 403 |
| AspS9I | GGNCC | 2 cut(s) | 193, 258 |
| AsuC2I | CCSGG | 3 cut(s) | 197, 276, 277 |
| AsuHPI | GGTGA | 1 cut(s) | 179 |
| AvaI | CYCGRG | 3 cut(s) | 218, 275, 388 |
| AvaII | GGWCC | 1 cut(s) | 258 |
| AvrII | CCTAGG | 1 cut(s) | 236 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 73, 106 |
| BamHI | GGATCC | 1 cut(s) | 119 |
| BauI | CACGAG | 1 cut(s) | 407 |
| BbsI | GAAGAC | 1 cut(s) | 39 |
| BbvI | GCAGC | 1 cut(s) | 390 |
| BccI | CCATC | 2 cut(s) | 248, 356 |
| BciT130I | CCWGG | 2 cut(s) | 16, 55 |
| BciVI | GTATCC | 1 cut(s) | 19 |
| BcnI | CCSGG | 3 cut(s) | 197, 276, 277 |
| BfaI | CTAG | 1 cut(s) | 237 |
| BfoI | RGCGCY | 1 cut(s) | 404 |
| BfuI | GTATCC | 1 cut(s) | 19 |
| BisI | GCNGC | 2 cut(s) | 375, 404 |
| BlnI | CCTAGG | 1 cut(s) | 236 |
| BlsI | GCNGC | 2 cut(s) | 376, 405 |
| Bme1390I | CCNGG | 5 cut(s) | 16, 55, 197, 276, 277 |
| Bme18I | GGWCC | 1 cut(s) | 258 |
| BmeT110I | CYCGRG | 3 cut(s) | 218, 275, 388 |
| BmgT120I | GGNCC | 2 cut(s) | 193, 258 |
| BmiI | GGNNCC | 3 cut(s) | 121, 195, 259 |
| BmrFI | CCNGG | 5 cut(s) | 16, 55, 197, 276, 277 |
| BmrI | ACTGGG | 1 cut(s) | 138 |
| BmsI | GCATC | 1 cut(s) | 300 |
| BmuI | ACTGGG | 1 cut(s) | 138 |
| BpiI | GAAGAC | 1 cut(s) | 39 |
| Bpu10I | CCTNAGC | 1 cut(s) | 347 |
| BpuMI | CCSGG | 3 cut(s) | 197, 276, 277 |
| Bsa29I | ATCGAT | 1 cut(s) | 75 |
| BsaBI | GATNNNNATC | 1 cut(s) | 75 |
| BsaJI | CCNNGG | 6 cut(s) | 15, 195, 219, 236, 275, 389 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 21, 129, 393, 394 |
| Bse1I | ACTGG | 2 cut(s) | 43, 133 |
| Bse8I | GATNNNNATC | 1 cut(s) | 75 |
| BseBI | CCWGG | 2 cut(s) | 16, 55 |
| BseCI | ATCGAT | 1 cut(s) | 75 |
| BseDI | CCNNGG | 6 cut(s) | 15, 195, 219, 236, 275, 389 |
| BseGI | GGATG | 2 cut(s) | 259, 367 |
| BseJI | GATNNNNATC | 1 cut(s) | 75 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 21, 129, 393, 394 |
| BseMII | CTCAG | 1 cut(s) | 308 |
| BseNI | ACTGG | 2 cut(s) | 43, 133 |
| BseRI | GAGGAG | 1 cut(s) | 167 |
| BseXI | GCAGC | 1 cut(s) | 390 |
| BsgI | GTGCAG | 1 cut(s) | 389 |
| Bsh1236I | CGCG | 3 cut(s) | 308, 338, 377 |
| Bsh1285I | CGRYCG | 1 cut(s) | 79 |
| BshFI | GGCC | 2 cut(s) | 194, 352 |
| BshVI | ATCGAT | 1 cut(s) | 75 |
| BsiEI | CGRYCG | 1 cut(s) | 79 |
| BsiHKCI | CYCGRG | 3 cut(s) | 218, 275, 388 |
| BsiSI | CCGG | 2 cut(s) | 197, 276 |
| BslFI | GGGAC | 1 cut(s) | 271 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 21, 129, 393, 394 |
| BsmFI | GGGAC | 1 cut(s) | 271 |
| BsnI | GGCC | 2 cut(s) | 194, 352 |
| BsoBI | CYCGRG | 3 cut(s) | 218, 275, 388 |
| Bsp143I | GATC | 6 cut(s) | 32, 76, 97, 119, 182, 300 |
| Bsp68I | TCGCGA | 1 cut(s) | 308 |
| BspACI | CCGC | 4 cut(s) | 188, 251, 338, 375 |
| BspANI | GGCC | 2 cut(s) | 194, 352 |
| BspCNI | CTCAG | 1 cut(s) | 309 |
| BspDI | ATCGAT | 1 cut(s) | 75 |
| BspFNI | CGCG | 3 cut(s) | 308, 338, 377 |
| BspLI | GGNNCC | 3 cut(s) | 121, 195, 259 |
| BspPI | GGATC | 4 cut(s) | 40, 114, 127, 190 |
| BsrI | ACTGG | 2 cut(s) | 43, 133 |
| BssECI | CCNNGG | 6 cut(s) | 15, 195, 219, 236, 275, 389 |
| BssMI | GATC | 6 cut(s) | 32, 76, 97, 119, 182, 300 |
| BssSI | CACGAG | 1 cut(s) | 407 |
| BssT1I | CCWWGG | 1 cut(s) | 236 |
| Bst2BI | CACGAG | 1 cut(s) | 407 |
| Bst2UI | CCWGG | 2 cut(s) | 16, 55 |
| Bst4CI | ACNGT | 2 cut(s) | 208, 262 |
| BstC8I | GCNNGC | 2 cut(s) | 60, 372 |
| BstDEI | CTNAG | 4 cut(s) | 85, 317, 347, 356 |
| BstF5I | GGATG | 2 cut(s) | 259, 367 |
| BstFNI | CGCG | 3 cut(s) | 308, 338, 377 |
| BstH2I | RGCGCY | 1 cut(s) | 404 |
| BstHHI | GCGC | 1 cut(s) | 403 |
| BstKTI | GATC | 6 cut(s) | 35, 79, 100, 122, 185, 303 |
| BstMBI | GATC | 6 cut(s) | 32, 76, 97, 119, 182, 300 |
| BstMCI | CGRYCG | 1 cut(s) | 79 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 59, 383 |
| BstNI | CCWGG | 2 cut(s) | 16, 55 |
| BstSCI | CCNGG | 5 cut(s) | 14, 53, 195, 274, 275 |
| BstUI | CGCG | 3 cut(s) | 308, 338, 377 |
| BstV1I | GCAGC | 1 cut(s) | 390 |
| BstV2I | GAAGAC | 1 cut(s) | 39 |
| BstX2I | RGATCY | 2 cut(s) | 119, 182 |
| BstYI | RGATCY | 2 cut(s) | 119, 182 |
| Bsu15I | ATCGAT | 1 cut(s) | 75 |
| BsuI | GTATCC | 1 cut(s) | 19 |
| BsuRI | GGCC | 2 cut(s) | 194, 352 |
| BsuTUI | ATCGAT | 1 cut(s) | 75 |
| BtgZI | GCGATG | 2 cut(s) | 289, 323 |
| BtsCI | GGATG | 2 cut(s) | 259, 367 |
| BtsI | GCAGTG | 1 cut(s) | 154 |
| BtsIMutI | CAGTG | 1 cut(s) | 154 |
| BtuMI | TCGCGA | 1 cut(s) | 308 |
| Cac8I | GCNNGC | 2 cut(s) | 60, 372 |
| CfoI | GCGC | 1 cut(s) | 403 |
| Cfr13I | GGNCC | 2 cut(s) | 193, 258 |
| Cfr9I | CCCGGG | 1 cut(s) | 275 |
| ClaI | ATCGAT | 1 cut(s) | 75 |
| CseI | GACGC | 1 cut(s) | 344 |
| Csp6I | GTAC | 2 cut(s) | 40, 82 |
| CviJI | RGCY | 7 cut(s) | 14, 62, 194, 235, 346, 352, 370 |
| CviKI_1 | RGCY | 7 cut(s) | 14, 62, 194, 235, 346, 352, 370 |
| CviQI | GTAC | 2 cut(s) | 40, 82 |
| DdeI | CTNAG | 4 cut(s) | 85, 317, 347, 356 |
| DpnI | GATC | 6 cut(s) | 34, 78, 99, 121, 184, 302 |
| DpnII | GATC | 6 cut(s) | 32, 76, 97, 119, 182, 300 |
| Eco130I | CCWWGG | 1 cut(s) | 236 |
| Eco147I | AGGCCT | 1 cut(s) | 352 |
| Eco32I | GATATC | 1 cut(s) | 73 |
| Eco47I | GGWCC | 1 cut(s) | 258 |
| Eco57I | CTGAAG | 1 cut(s) | 84 |
| Eco88I | CYCGRG | 3 cut(s) | 218, 275, 388 |
| EcoO109I | RGGNCCY | 1 cut(s) | 193 |
| EcoRII | CCWGG | 2 cut(s) | 14, 53 |
| EcoRV | GATATC | 1 cut(s) | 73 |
| EcoT14I | CCWWGG | 1 cut(s) | 236 |
| ErhI | CCWWGG | 1 cut(s) | 236 |
| FaiI | YATR | 4 cut(s) | 227, 243, 265, 287 |
| FaqI | GGGAC | 1 cut(s) | 271 |
| Fnu4HI | GCNGC | 2 cut(s) | 375, 404 |
| FokI | GGATG | 2 cut(s) | 266, 374 |
| Fsp4HI | GCNGC | 2 cut(s) | 375, 404 |
| FspBI | CTAG | 1 cut(s) | 237 |
| GlaI | GCGC | 1 cut(s) | 402 |
| GluI | GCNGC | 2 cut(s) | 375, 404 |
| HaeII | RGCGCY | 1 cut(s) | 404 |
| HaeIII | GGCC | 2 cut(s) | 194, 352 |
| HapII | CCGG | 2 cut(s) | 197, 276 |
| HgaI | GACGC | 1 cut(s) | 344 |
| HhaI | GCGC | 1 cut(s) | 403 |
| Hin6I | GCGC | 1 cut(s) | 401 |
| HinP1I | GCGC | 1 cut(s) | 401 |
| HinfI | GANTC | 4 cut(s) | 5, 155, 379, 412 |
| HpaII | CCGG | 2 cut(s) | 197, 276 |
| HphI | GGTGA | 1 cut(s) | 179 |
| Hpy188I | TCNGA | 2 cut(s) | 10, 142 |
| Hpy188III | TCNNGA | 2 cut(s) | 218, 307 |
| Hpy99I | CGWCG | 2 cut(s) | 40, 272 |
| HpyCH4III | ACNGT | 2 cut(s) | 208, 262 |
| HpyCH4IV | ACGT | 1 cut(s) | 162 |
| HpyCH4V | TGCA | 2 cut(s) | 245, 406 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 59, 383 |
| HpyF3I | CTNAG | 4 cut(s) | 85, 317, 347, 356 |
| HpySE526I | ACGT | 1 cut(s) | 162 |
| HspAI | GCGC | 1 cut(s) | 401 |
| Kzo9I | GATC | 6 cut(s) | 32, 76, 97, 119, 182, 300 |
| LmnI | GCTCC | 1 cut(s) | 343 |
| LpnPI | CCDG | 9 cut(s) | 28, 40, 56, 67, 114, 210, 289, 317, 396 |
| Lsp1109I | GCAGC | 1 cut(s) | 390 |
| LweI | GCATC | 1 cut(s) | 300 |
| MaeI | CTAG | 1 cut(s) | 237 |
| MaeII | ACGT | 1 cut(s) | 162 |
| MaeIII | GTNAC | 1 cut(s) | 202 |
| MalI | GATC | 6 cut(s) | 34, 78, 99, 121, 184, 302 |
| MboI | GATC | 6 cut(s) | 32, 76, 97, 119, 182, 300 |
| MboII | GAAGA | 1 cut(s) | 39 |
| MflI | RGATCY | 2 cut(s) | 119, 182 |
| MluCI | AATT | 2 cut(s) | 101, 213 |
| MlyI | GAGTC | 1 cut(s) | 164 |
| MmeI | TCCRAC | 1 cut(s) | 183 |
| MnlI | CCTC | 7 cut(s) | 145, 169, 184, 214, 312, 363, 384 |
| MroXI | GAANNNNTTC | 1 cut(s) | 416 |
| MslI | CAYNNNNRTG | 1 cut(s) | 308 |
| MspI | CCGG | 2 cut(s) | 197, 276 |
| MspR9I | CCNGG | 5 cut(s) | 16, 55, 197, 276, 277 |
| MvaI | CCWGG | 2 cut(s) | 16, 55 |
| MvnI | CGCG | 3 cut(s) | 308, 338, 377 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 59, 383 |
| NciI | CCSGG | 3 cut(s) | 197, 276, 277 |
| NdeII | GATC | 6 cut(s) | 32, 76, 97, 119, 182, 300 |
| NlaIV | GGNNCC | 3 cut(s) | 121, 195, 259 |
| NmuCI | GTSAC | 1 cut(s) | 202 |
| NruI | TCGCGA | 1 cut(s) | 308 |
| PceI | AGGCCT | 1 cut(s) | 352 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 35 |
| PdmI | GAANNNNTTC | 1 cut(s) | 416 |
| PfeI | GAWTC | 3 cut(s) | 5, 379, 412 |
| PkrI | GCNGC | 2 cut(s) | 376, 405 |
| Ple19I | CGATCG | 1 cut(s) | 79 |
| PleI | GAGTC | 1 cut(s) | 163 |
| PpsI | GAGTC | 1 cut(s) | 163 |
| Psp6I | CCWGG | 2 cut(s) | 14, 53 |
| PspGI | CCWGG | 2 cut(s) | 14, 53 |
| PspN4I | GGNNCC | 3 cut(s) | 121, 195, 259 |
| PspPI | GGNCC | 2 cut(s) | 193, 258 |
| PsuI | RGATCY | 2 cut(s) | 119, 182 |
| PvuI | CGATCG | 1 cut(s) | 79 |
| RruI | TCGCGA | 1 cut(s) | 308 |
| RsaI | GTAC | 2 cut(s) | 41, 83 |
| RsaNI | GTAC | 2 cut(s) | 40, 82 |
| RseI | CAYNNNNRTG | 1 cut(s) | 308 |
| SatI | GCNGC | 2 cut(s) | 375, 404 |
| Sau3AI | GATC | 6 cut(s) | 32, 76, 97, 119, 182, 300 |
| Sau96I | GGNCC | 2 cut(s) | 193, 258 |
| SchI | GAGTC | 1 cut(s) | 164 |
| ScrFI | CCNGG | 5 cut(s) | 16, 55, 197, 276, 277 |
| SetI | ASST | 8 cut(s) | 64, 91, 165, 242, 323, 348, 372, 395 |
| SfaNI | GCATC | 1 cut(s) | 300 |
| SinI | GGWCC | 1 cut(s) | 258 |
| SmaI | CCCGGG | 1 cut(s) | 277 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 308 |
| Sse9I | AATT | 2 cut(s) | 101, 213 |
| SseBI | AGGCCT | 1 cut(s) | 352 |
| SsiI | CCGC | 4 cut(s) | 188, 251, 338, 375 |
| SspMI | CTAG | 1 cut(s) | 237 |
| StuI | AGGCCT | 1 cut(s) | 352 |
| StyD4I | CCNGG | 5 cut(s) | 14, 53, 195, 274, 275 |
| StyI | CCWWGG | 1 cut(s) | 236 |
| TaaI | ACNGT | 2 cut(s) | 208, 262 |
| TaiI | ACGT | 1 cut(s) | 165 |
| TaqI | TCGA | 2 cut(s) | 75, 91 |
| TasI | AATT | 2 cut(s) | 101, 213 |
| TauI | GCSGC | 1 cut(s) | 377 |
| TfiI | GAWTC | 3 cut(s) | 5, 379, 412 |
| TscAI | CASTG | 1 cut(s) | 154 |
| TseFI | GTSAC | 1 cut(s) | 202 |
| TseI | GCWGC | 1 cut(s) | 403 |
| Tsp45I | GTSAC | 1 cut(s) | 202 |
| TspDTI | ATGAA | 1 cut(s) | 308 |
| TspMI | CCCGGG | 1 cut(s) | 275 |
| TspRI | CASTG | 1 cut(s) | 154 |
| VpaK11BI | GGWCC | 1 cut(s) | 258 |
| XmaI | CCCGGG | 1 cut(s) | 275 |
| XmaJI | CCTAGG | 1 cut(s) | 236 |
| XmnI | GAANNNNTTC | 1 cut(s) | 416 |
| XspI | CTAG | 1 cut(s) | 237 |