MD09G1086500.v1.1

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000027
Physical Location & Seq
Forward (+)
6198123 .. 6204745
6623 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1086500.v1.1.491

Sequence Viewer

Length: 2490 bp
ATGACAACTACAATTGTTCCTACACTTCTTAACCGTAAAAACTATGACGATTGGAGTTCTCGGATTAAGAGCTACTTGTTGGCCGAAGATCTTTGGGACGTTGTCGAACGCAAGTATGACAACAACAACAACGAGGCTTTGAGGAAGAAGGATGCTAAAGCCTTATATGCAATCGAGAATTCTTGCGGGAAAGATGCGTATAAACTTATCAAGGACGCAGACACGGCCAATGGCGCATGGGATACTTTGCAAGAAACTTTGAGACCAGCATATAATCGATCATCAGATGAACAAGGTATGAAGACAATTGTTGAAGCGTATATGTCTCTGACTCTGACCCCACTGGTGGTAATGGAAGAAGGGCGCACTGGAGGGCATGATAATATCCGTGAAACTTTCGTCAAATATGTGGAGTCTAATGATTGGGATAATGCGATCGAGTTTCTTAGCCAACATCGCCAGCTAGGAAGTGAAAGAATTTTTCATGACCGTACAGCTCTTCACTGCGCAGTAAATGGGTTAAGCAAGTGCAGCGTGCGCAATATGGAACAGTTGATGGAGTTAATGGCAAAGAAAGACTTGGCAATACAAGATATCTTTGGCTGTACAGCTCTTCACGACGCAGTTTGTGATTCTAGAGAGTTCAGCGTGCGCATTGTGGAAAAGTTAGTGGACTTAATGGAAAAGAAAGACCTGGCAATACAAGACTTCCGTTGCCGTACAGCTCTTCACAACGCACTCTGTGAGCCTAGCAAGTTGAGCGTGCGCATTGTGAAAAAGTTGGTGGACTTAATGGAAAAGGAAGACTTGGCAATACAAGATGATCTTGGTCGCACAGCTCTTCATTATTTAGTAAGAGAATATCCAGAAAGGGTTGAAGTAGCCAAATGCATGGTTGATAAGAACTACAATTTACCTACCATTTTTACTACAAGTATGCCTCCAGTTGTCGTGGCTGAGATGCAGGAAAAAGGAAAAAGAATGGCTCGCTATCTCTATTCCCTCACTCCACGCCAAACACTAAGAGTTCGCGATGGAGCTCAACTTATTTCTAGCGGTTTTCTTCACAAGAGATTTGATATTGTCTGGGATTTGATTCAACGTTATCCACTGTTGACCTTGGCTAAAGACCACAATGGAGATTTCCCCTTAGTAACATTGGTAAAAAACCGTTCAGCATTCTTAAGTGGAAGCCAACTCACCTTATGGGAAAAGTTGATCTATTATGGTATACGCATAAAACCTCTTCCTCCCATTAATGAAAATGGCCTGAGTGATCAAAGGCCTCTCTCAAACCAAAAAAAGAATCAAAGGCATCTAATCTCCTCAGGAATAATCAATCGTATATATGAGATGAAATTGATGCATGAGCGGGTCCATCAGTTTTTACCTCTCATATTTAAAGCCGCAACGGATGTAGTTCCATCAGTTTCTACCCCTCACATTATTGAAGGCTTGCATGAAGCATTGCGTGTTGCAGCAGAATGTGGGCATGCAGAGTATTTTGTTCATAGGTTTAATGTCGGAATGGATGAGTCCTTTAAATCAAATGATGATGTTTACTCAATTTCATTAAATGATCAAGTGAACAGGACAAACCAGAGCATGTTCCATATTGCTGCTGAATGTCGTCACCACAAACTTTATAATCTTATATATGTCATCCATGAGTTGAGCCAAGAAAAAAAAGAAGATAAAGATGGCCAAAAACGATTCCGTCAAATGCAAGGGGCAGTGGGGCGGAAAGATTACTTTGGCAATAATGTGCTACATACTGTGGCAAGTATTACCTCAGTTTCACGGATTGATCACATTCGAGGCGCAGCTTTGCAAATGCAAAGAGAACTACAATGGTTCAAGGAGGTGGAGAGCGTTACAGATCCCAAAGATCGTGAATGTCTAAATGATGATGGTATGACACCACAAGAATTATTTAAGGAGAATCATAAAAAAATGGGGGAAGAGGCAGAAAAGTCAATGAAAGAAACAGCAACTTCGTGTACAGTTGTAGGTGCTCTCATTGTCACCATCATGTTTGCTGCAGCATTCACAGTTCCTGGTGGTAATGATGAAAAGACAAGTCTTCCTAAATTCTTAACTAAAAAAGTATTCACAGCATTTATAGTTTCGGATGCAATCTCGCTCTTTTCTTCAACAACTTCGGTTATCATGTTTTTAGGCATTCTCACGTCACGTTATTCTGAAGATGATTTCTTCAAATCTTTGCCCACGAAAATGATAATAGGCCTTTTCACCCTCTTCTTATCTATCACCACCATGATGATTGTCTTTTCTTGTGCCCTTTACATTATGCTTGACGGGAAATCGTCGATTGTTATTCCAATCATTTTGATTGCCAGTGTTCCAGTTGCCTCCTTCATCTCGATGCAATTTCCGTTGCTTGTCGACATATTTATTTTTACGTATGGACCAAGTATGTTTGGTGAGAACAACAAAACATGGTTTAAATTCCTCTGTGATCTTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 DUF4219 PGG

Protein Analysis

830

Amino Acids

94.68

Weight (kDa)

6.78

Isoelectric Point (pI)

36.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4219 PF13961 10 - 36 2.4e-09 Domain of unknown function (DUF4219)
Retrotran_gag_2 PF14223 23 - 86 5e-08 gag-polypeptide of LTR copia-type
PGG PF13962 658 - 770 2.1e-23 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000027)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18670 AT3G18670 AT3G54065
fragaria_vesca FvH4_1g26570 FvH4_1g26570 FvH4_5g19270 FvH4_5g19273 FvH4_5g35840 FvH4_5g36340 FvH4_5g36630 FvH4_5g37181 FvH4_5g37182 FvH4_5g37185 FvH4_5g37240 FvH4_5g37260 FvH4_6g21440 FvH4_6g44571 FvH4_6g44572 FvH4_6g44610 FvH4_6g44620 FvH4_6g44650 FvH4_6g44660 FvH4_6g45151 FvH4_6g45152 FvH4_6g45152 FvH4_6g45153 FvH4_6g45153 FvH4_6g45153 FvH4_6g45153 FvH4_6g45153 FvH4_6g45154 FvH4_6g51440 FvH4_7g08090 FvH4_7g08090 FvH4_7g08090 FvH4_7g08090 FvH4_7g08090 FvH4_7g08090
malus_domestica MD00G1010300.v1.1 MD00G1010400.v1.1 MD00G1010500.v1.1 MD00G1011000.v1.1 MD00G1011200.v1.1 MD00G1011500.v1.1 MD00G1011900.v1.1 MD03G1133900.v1.1 MD03G1134100.v1.1 MD09G1086300.v1.1 MD09G1086500.v1.1 MD09G1086900.v1.1 MD09G1087000.v1.1 MD09G1088000.v1.1 MD09G1088100.v1.1 MD09G1088200.v1.1 MD09G1088300.v1.1 MD09G1089000.v1.1 MD09G1089300.v1.1 MD09G1133800.v1.1 MD16G1219400.v1.1 MD17G1027900.v1.1 MD17G1028400.v1.1 MD17G1028600.v1.1 MD17G1028700.v1.1 MD17G1028900.v1.1 MD17G1029000.v1.1 MD17G1029100.v1.1 MD17G1029200.v1.1 MD17G1075400.v1.1 MD17G1076000.v1.1 MD17G1077000.v1.1 MD17G1077100.v1.1 MD17G1077800.v1.1 MD17G1078000.v1.1 MD17G1078100.v1.1 MD17G1078300.v1.1 MD17G1100900.v1.1 MD17G1101100.v1.1 MD17G1101400.v1.1 MD17G1104200.v1.1 MD17G1104500.v1.1 MD17G1121300.v1.1 MD17G1121600.v1.1 MD17G1121900.v1.1 MD17G1122700.v1.1 MD17G1218800.v1.1
prunus_persica Prupe.2G033800_v2.0.a1 Prupe.2G033900_v2.0.a1 Prupe.2G080300_v2.0.a1 Prupe.2G080700_v2.0.a1 Prupe.2G080900_v2.0.a1 Prupe.2G081100_v2.0.a1 Prupe.2G081100_v2.0.a1 Prupe.2G081200_v2.0.a1 Prupe.2G081200_v2.0.a1 Prupe.2G081200_v2.0.a1 Prupe.2G081200_v2.0.a1 Prupe.2G081700_v2.0.a1 Prupe.2G081700_v2.0.a1 Prupe.2G082000_v2.0.a1 Prupe.2G082100_v2.0.a1 Prupe.2G082100_v2.0.a1 Prupe.2G082200_v2.0.a1 Prupe.2G082300_v2.0.a1 Prupe.2G082700_v2.0.a1 Prupe.2G082800_v2.0.a1 Prupe.2G082900_v2.0.a1 Prupe.3G235600_v2.0.a1 Prupe.3G236300_v2.0.a1 Prupe.3G236300_v2.0.a1 Prupe.3G236600_v2.0.a1 Prupe.3G236600_v2.0.a1 Prupe.3G236800_v2.0.a1 Prupe.3G237000_v2.0.a1 Prupe.3G237100_v2.0.a1 Prupe.3G237300_v2.0.a1 Prupe.3G237400_v2.0.a1 Prupe.3G237500_v2.0.a1 Prupe.3G237500_v2.0.a1 Prupe.3G237500_v2.0.a1 Prupe.4G282500_v2.0.a1 Prupe.4G282500_v2.0.a1
pyrus_communis pycom03g08990 pycom03g09000 pycom09g01180 pycom09g01190 pycom09g01220 pycom09g01230 pycom09g01360 pycom09g01370 pycom09g01380 pycom09g01410 pycom09g05500 pycom11g10280 pycom11g10290 pycom11g10310 pycom16g18270 pycom16g18280 pycom17g02280 pycom17g02290 pycom17g02300 pycom17g02360 pycom17g02390 pycom17g02410 pycom17g02440 pycom17g02460 pycom17g02470 pycom17g02480 pycom17g02510 pycom17g02530 pycom17g02540 pycom17g02550 pycom17g02560 pycom17g02570 pycom17g02580 pycom17g02630 pycom17g02640 pycom17g04970 pycom17g04980 pycom17g05010 pycom17g07090 pycom17g07620 pycom17g07630 pycom17g07640 pycom17g07650 pycom17g07660 pycom17g07740 pycom17g07760 pycom17g07770 pycom17g09580 pycom17g11170 pycom17g11200 pycom17g11220 pycom17g11240 pycom17g11250 pycom17g11300 pycom17g11340 pycom17g11380 pycom17g11440
rosa_chinensis RchiOBHm_Chr1g0332901 RchiOBHm_Chr1g0338981 RchiOBHm_Chr1g0338991 RchiOBHm_Chr1g0341201 RchiOBHm_Chr1g0341211 RchiOBHm_Chr1g0342721 RchiOBHm_Chr1g0343191 RchiOBHm_Chr2g0146761 RchiOBHm_Chr2g0161791 RchiOBHm_Chr2g0161801 RchiOBHm_Chr2g0161841 RchiOBHm_Chr2g0162041 RchiOBHm_Chr2g0162651 RchiOBHm_Chr2g0162691 RchiOBHm_Chr2g0162701 RchiOBHm_Chr2g0162711 RchiOBHm_Chr2g0162721 RchiOBHm_Chr2g0162811 RchiOBHm_Chr2g0162831 RchiOBHm_Chr2g0162841 RchiOBHm_Chr4g0416381 RchiOBHm_Chr4g0416391 RchiOBHm_Chr5g0075981 RchiOBHm_Chr6g0276101 RchiOBHm_Chr7g0214581 RchiOBHm_Chr7g0214631 RchiOBHm_Chr7g0214651 RchiOBHm_Chr7g0214721 RchiOBHm_Chr7g0223161 RchiOBHm_Chr7g0230661 RchiOBHm_Chr7g0230751 RchiOBHm_Chr7g0230781 RchiOBHm_Chr7g0234581 RchiOBHm_Chr7g0234601 RchiOBHm_Chr7g0237561 RchiOBHm_Chr7g0237571 RchiOBHm_Chr7g0238781 RchiOBHm_Chr7g0238811 RchiOBHm_Chr7g0242161 RchiOBHm_Chr7g0242401 RchiOBHm_Chr7g0242451 RchiOBHm_Chr7g0242921 RchiOBHm_Chr7g0242981 RchiOBHm_Chr7g0243111 RchiOBHm_Chr7g0243251 RchiOBHm_Chr7g0243331
rosa_laevigata RLG00000000803 RLG00000000807 RLG00000000809 RLG00000000811 RLG00000000814 RLG00000000854 RLG00000000859 RLG00000000864 RLG00000000994 RLG00000000995 RLG00000001458 RLG00000001461 RLG00000001474 RLG00000001476 RLG00000002729 RLG00000002730 RLG00000003505 RLG00000008871 RLG00000008873 RLG00000018513 RLG00000021300 RLG00000021303 RLG00000021318 RLG00000021356 RLG00000021357 RLG00000029070 RLG00000029071 RLG00000029171 RLG00000029189 RLG00000029190 RLG00000029640 RLG00000034635
rosa_multiflora Rmu_co8149086.1_g000001 Rmu_co8460577.1_g000001 Rmu_sc0000429.1_g000062 Rmu_sc0000429.1_g000071 Rmu_sc0000429.1_g000081 Rmu_sc0000863.1_g000020 Rmu_sc0001488.1_g000011 Rmu_sc0001545.1_g000007 Rmu_sc0001849.1_g000068 Rmu_sc0001993.1_g000003 Rmu_sc0002222.1_g000002 Rmu_sc0002308.1_g000053 Rmu_sc0002308.1_g000069 Rmu_sc0002480.1_g000007 Rmu_sc0003179.1_g000041 Rmu_sc0004213.1_g000011 Rmu_sc0007101.1_g000012 Rmu_sc0008565.1_g000011 Rmu_sc0009298.1_g000003 Rmu_sc0010407.1_g000009 Rmu_sc0010407.1_g000013 Rmu_sc0026966.1_g000004 Rmu_ssc0000152.1_g000012 Rmu_ssc0000152.1_g000014 Rmu_ssc0000295.1_g000021 Rmu_ssc0000295.1_g000026
rosa_roxburghii Rroxscaffold_1G00029600 Rroxscaffold_2G00087790 Rroxscaffold_2G00087800 Rroxscaffold_2G00088360 Rroxscaffold_2G00088500 Rroxscaffold_2G00088530 Rroxscaffold_2G00088590 Rroxscaffold_3G00221570 Rroxscaffold_3G00222190 Rroxscaffold_3G00222240 Rroxscaffold_3G00222880 Rroxscaffold_3G00222900 Rroxscaffold_3G00223990 Rroxscaffold_3G00229750 Rroxscaffold_3G00229760 Rroxscaffold_3G00245140 Rroxscaffold_3G00245150 Rroxscaffold_3G00245200 Rroxscaffold_3G00253520 Rroxscaffold_3G00253530 Rroxscaffold_4G00294500 Rroxscaffold_4G00311800 Rroxscaffold_4G00311830 Rroxscaffold_4G00311850 Rroxscaffold_4G00313180 Rroxscaffold_4G00313210 Rroxscaffold_4G00316270 Rroxscaffold_4G00317910 Rroxscaffold_4G00317920 Rroxscaffold_5G00349290 Rroxscaffold_5G00349300 Rroxscaffold_5G00349310 Rroxscaffold_5G00351950 Rroxscaffold_5G00351960 Rroxscaffold_5G00353390 Rroxscaffold_7G00192540
rosa_rugosa Rorug01G0102500 Rorug01G0102600 Rorug01G0146400.1 Rorug01G0146500.1 Rorug01G0159400.1 Rorug01G0159500.1 Rorug02G0491900 Rorug02G0492000 Rorug02G0492100 Rorug02G0492200 Rorug02G0492300 Rorug02G0492400 Rorug02G0492500 Rorug02G0492700 Rorug02G0498500 Rorug02G0498600 Rorug02G0498800 Rorug04G0006900 Rorug04G0053700 Rorug06G0002000 Rorug06G0002100 Rorug07G0304700 Rorug07G0304800 Rorug07G0304800 Rorug07G0304800 Rorug07G0305300 Rorug07G0305400 Rorug07G0305500 Rorug07G0305500 Rorug07G0318300 Rorug07G0318600 Rorug07G0323600
rosa_samantha Rh1AG128900 Rh1AG162500 Rh1AG163000 Rh1AG164000 Rh1AG164700 Rh1AG173200 Rh1AG173300 Rh1AG173800 Rh1BG098100 Rh1BG129800 Rh1BG130600 Rh1BG141300 Rh1BG141500 Rh1BG141600 Rh1CG122700 Rh1CG151900 Rh1CG152400 Rh1CG153100 Rh1CG153500 Rh1CG161400 Rh1CG161600 Rh1DG134200 Rh1DG173500 Rh1DG173700 Rh2AG558100 Rh2AG558500 Rh2AG560200 Rh2AG564700 Rh2AG564800 Rh2BG465600 Rh2BG571700 Rh2BG572000 Rh2BG573500 Rh2CG439800 Rh2CG541800 Rh2CG542100 Rh2CG542200 Rh2CG543800 Rh2CG547300 Rh2DG474700 Rh2DG580600 Rh2DG580900 Rh2DG581200 Rh2DG581700 Rh2DG581800 Rh2DG583500 Rh2DG587200 Rh2DG587300 Rh2DG587400 Rh4AG128900 Rh4AG203800 Rh4AG319900 Rh4AG320000 Rh4BG122200 Rh4BG201100 Rh4CG136000 Rh4DG122400 Rh4DG201100 Rh4DG323600 Rh6BG077000 Rh6DG206200 Rh7AG282300 Rh7AG282500 Rh7AG283400 Rh7AG358600 Rh7AG414500 Rh7AG415200 Rh7AG415500 Rh7AG461700 Rh7AG469300 Rh7AG477800 Rh7BG273900 Rh7BG274000 Rh7BG390400 Rh7BG390500 Rh7BG391500 Rh7BG391800 Rh7BG408300 Rh7BG432000 Rh7BG439200 Rh7BG449200 Rh7BG449900 Rh7BG450400 Rh7BG450500 Rh7CG220900 Rh7CG301600 Rh7CG302700 Rh7CG377000 Rh7CG433900 Rh7CG434200 Rh7CG478800 Rh7DG286200 Rh7DG354000 Rh7DG424600 Rh7DG448000 Rh7DG462800 Rh7DG462900
rosa_wichuraiana Rw0G003440 Rw0G011970 Rw0G017120 Rw0G017130 Rw0G017140 Rw0G018470 Rw0G018480 Rw0G020440 Rw1G010470 Rw2G046180 Rw2G046210 Rw2G046230 Rw2G046390 Rw2G046400 Rw2G046710 Rw2G046720 Rw2G046730 Rw3G025310 Rw4G010410 Rw4G017250 Rw6G018270 Rw7G018180 Rw7G024130 Rw7G024160 Rw7G024170 Rw7G034060 Rw7G034250 Rw7G034260 Rw7G034400 Rw7G034410 Rw7G038210 Rw7G039020 Rw7G039060 Rw7G039280 Rw7G039400 Rw7G039470 Rw7G039880 Rw7G039890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1647
Acc16I TGCGCA 4 cut(s) 508, 539, 653, 767
AccBSI CCGCTC 1 cut(s) 1372
AccI GTMKAC 2 cut(s) 1231, 2406
AccII CGCG 1 cut(s) 1032
AciI CCGC 5 cut(s) 186, 1056, 1372, 1407, 1741
AclI AACGTT 1 cut(s) 1102
AclWI GGATC 1 cut(s) 1874
AcoI YGGCCR 3 cut(s) 81, 225, 1702
AcsI RAATTY 4 cut(s) 178, 477, 2090, 2468
AcuI CTGAAG 1 cut(s) 2223
AdeI CACNNNGTG 1 cut(s) 743
AfaI GTAC 4 cut(s) 493, 607, 721, 2002
AfiI CCNNNNNNNGG 1 cut(s) 346
AflII CTTAAG 1 cut(s) 1183
AgsI TTSAA 7 cut(s) 314, 878, 1100, 1451, 1858, 2154, 2218
AjiI CACGTC 1 cut(s) 2190
AjnI CCWGG 2 cut(s) 693, 2056
AluBI AGCT 8 cut(s) 72, 463, 497, 611, 725, 839, 1040, 1826
AluI AGCT 8 cut(s) 72, 463, 497, 611, 725, 839, 1040, 1826
Alw21I GWGCWC 2 cut(s) 1042, 2017
Alw26I GTCTC 2 cut(s) 256, 330
AlwI GGATC 1 cut(s) 1874
AlwNI CAGNNNCTG 1 cut(s) 2057
AoxI GGCC 6 cut(s) 81, 225, 1267, 1283, 1702, 2245
ApeKI GCWGC 6 cut(s) 531, 1478, 1619, 1823, 2039, 2042
ApoI RAATTY 4 cut(s) 178, 477, 2090, 2468
AseI ATTAAT 1 cut(s) 1257
AspLEI GCGC 7 cut(s) 236, 366, 509, 540, 654, 768, 1823
AspS9I GGNCC 2 cut(s) 1375, 2429
AsuHPI GGTGA 6 cut(s) 1192, 1625, 2017, 2245, 2263, 2456
AvaII GGWCC 2 cut(s) 1375, 2429
AxyI CCTNAGG 1 cut(s) 1327
BaeGI GKGCMC 1 cut(s) 2302
BalI TGGCCA 1 cut(s) 1704
BanII GRGCYC 1 cut(s) 1042
BbsI GAAGAC 3 cut(s) 308, 810, 2075
Bbv12I GWGCWC 2 cut(s) 1042, 2017
BbvI GCAGC 6 cut(s) 543, 1490, 1606, 1835, 2026, 2054
BccI CCATC 7 cut(s) 550, 1028, 1386, 1432, 1694, 1904, 2036
BceAI ACGGC 2 cut(s) 240, 702
BciT130I CCWGG 2 cut(s) 695, 2058
BciVI GTATCC 1 cut(s) 235
BclI TGATCA 3 cut(s) 1276, 1579, 1807
BcoDI GTCTC 2 cut(s) 256, 330
BfaI CTAG 4 cut(s) 464, 636, 750, 1053
BfmI CTRYAG 1 cut(s) 2040
BfrI CTTAAG 1 cut(s) 1183
BfuI GTATCC 1 cut(s) 235
BglII AGATCT 1 cut(s) 88
BisI GCNGC 7 cut(s) 532, 1407, 1479, 1620, 1824, 2040, 2043
BlsI GCNGC 7 cut(s) 533, 1408, 1480, 1621, 1825, 2041, 2044
Bme1390I CCNGG 2 cut(s) 695, 2058
Bme18I GGWCC 2 cut(s) 1375, 2429
BmgBI CACGTC 1 cut(s) 2190
BmgT120I GGNCC 2 cut(s) 1375, 2429
BmiI GGNNCC 1 cut(s) 1376
BmrFI CCNGG 2 cut(s) 695, 2058
BmsI GCATC 7 cut(s) 142, 184, 951, 1324, 1353, 2122, 2376
BpiI GAAGAC 3 cut(s) 308, 810, 2075
BpmI CTGGAG 2 cut(s) 390, 927
Bsa29I ATCGAT 1 cut(s) 277
BsaAI YACGTR 1 cut(s) 2424
BsaI GGTCTC 1 cut(s) 256
BsaJI CCNNGG 1 cut(s) 1119
Bsc4I CCNNNNNNNGG 1 cut(s) 346
Bse1I ACTGG 5 cut(s) 348, 373, 944, 2358, 2366
Bse21I CCTNAGG 1 cut(s) 1327
Bse3DI GCAATG 1 cut(s) 1466
BseBI CCWGG 2 cut(s) 695, 2058
BseCI ATCGAT 1 cut(s) 277
BseDI CCNNGG 1 cut(s) 1119
BseGI GGATG 5 cut(s) 157, 1420, 1537, 1662, 2137
BseLI CCNNNNNNNGG 1 cut(s) 346
BseMI GCAATG 1 cut(s) 1466
BseMII CTCAG 4 cut(s) 948, 1262, 1341, 1806
BseNI ACTGG 5 cut(s) 348, 373, 944, 2358, 2366
BseRI GAGGAG 1 cut(s) 1315
BseSI GKGCMC 1 cut(s) 2302
BseXI GCAGC 6 cut(s) 543, 1490, 1606, 1835, 2026, 2054
BsgI GTGCAG 1 cut(s) 550
Bsh1236I CGCG 1 cut(s) 1032
Bsh1285I CGRYCG 1 cut(s) 438
BshFI GGCC 6 cut(s) 83, 227, 1269, 1285, 1704, 2247
BshVI ATCGAT 1 cut(s) 277
BsiEI CGRYCG 1 cut(s) 438
BsiHKAI GWGCWC 2 cut(s) 1042, 2017
BslFI GGGAC 1 cut(s) 110
BslI CCNNNNNNNGG 1 cut(s) 346
BsmAI GTCTC 2 cut(s) 256, 330
BsmFI GGGAC 1 cut(s) 110
BsmI GAATGC 3 cut(s) 1178, 2045, 2181
BsnI GGCC 6 cut(s) 83, 227, 1269, 1285, 1704, 2247
Bso31I GGTCTC 1 cut(s) 256
Bsp1286I GDGCHC 3 cut(s) 1042, 2017, 2302
Bsp1407I TGTACA 2 cut(s) 605, 2000
Bsp68I TCGCGA 1 cut(s) 1032
BspACI CCGC 5 cut(s) 186, 1056, 1372, 1407, 1741
BspANI GGCC 6 cut(s) 83, 227, 1269, 1285, 1704, 2247
BspCNI CTCAG 4 cut(s) 949, 1263, 1340, 1805
BspDI ATCGAT 1 cut(s) 277
BspFNI CGCG 1 cut(s) 1032
BspHI TCATGA 1 cut(s) 484
BspLI GGNNCC 1 cut(s) 1376
BspMAI CTGCAG 1 cut(s) 2044
BspPI GGATC 1 cut(s) 1874
BspQI GCTCTTC 4 cut(s) 504, 618, 732, 846
BspTI CTTAAG 1 cut(s) 1183
BspTNI GGTCTC 1 cut(s) 256
BsrBI CCGCTC 1 cut(s) 1372
BsrDI GCAATG 1 cut(s) 1466
BsrGI TGTACA 2 cut(s) 605, 2000
BsrI ACTGG 5 cut(s) 348, 373, 944, 2358, 2366
BssECI CCNNGG 1 cut(s) 1119
BssNAI GTATAC 1 cut(s) 1232
BssT1I CCWWGG 1 cut(s) 1119
Bst1107I GTATAC 1 cut(s) 1232
Bst2UI CCWGG 2 cut(s) 695, 2058
Bst4CI ACNGT 8 cut(s) 35, 491, 552, 1113, 1172, 1777, 2005, 2053
Bst6I CTCTTC 7 cut(s) 504, 618, 732, 846, 1251, 1956, 2264
BstAFI CTTAAG 1 cut(s) 1183
BstAUI TGTACA 2 cut(s) 605, 2000
BstBAI YACGTR 1 cut(s) 2424
BstC8I GCNNGC 7 cut(s) 461, 536, 650, 764, 988, 1457, 1494
BstDEI CTNAG 7 cut(s) 446, 957, 1022, 1150, 1271, 1327, 1792
BstF5I GGATG 5 cut(s) 157, 1420, 1537, 1662, 2137
BstFNI CGCG 1 cut(s) 1032
BstHHI GCGC 7 cut(s) 236, 366, 509, 540, 654, 768, 1823
BstMAI GTCTC 2 cut(s) 256, 330
BstMCI CGRYCG 1 cut(s) 438
BstMWI GCNNNNNNNGC 7 cut(s) 167, 224, 233, 456, 531, 537, 759
BstNI CCWGG 2 cut(s) 695, 2058
BstNSI RCATGY 2 cut(s) 1496, 1609
BstSCI CCNGG 2 cut(s) 693, 2056
BstSFI CTRYAG 1 cut(s) 2040
BstSLI GKGCMC 1 cut(s) 2302
BstSNI TACGTA 1 cut(s) 2424
BstUI CGCG 1 cut(s) 1032
BstV1I GCAGC 6 cut(s) 543, 1490, 1606, 1835, 2026, 2054
BstV2I GAAGAC 3 cut(s) 308, 810, 2075
BstX2I RGATCY 2 cut(s) 88, 1879
BstXI CCANNNNNNTGG 1 cut(s) 892
BstYI RGATCY 2 cut(s) 88, 1879
BstZ17I GTATAC 1 cut(s) 1232
Bsu15I ATCGAT 1 cut(s) 277
Bsu36I CCTNAGG 1 cut(s) 1327
BsuI GTATCC 1 cut(s) 235
BsuRI GGCC 6 cut(s) 83, 227, 1269, 1285, 1704, 2247
BsuTUI ATCGAT 1 cut(s) 277
BtgZI GCGATG 2 cut(s) 440, 1047
BtrI CACGTC 1 cut(s) 2190
BtsCI GGATG 5 cut(s) 157, 1420, 1537, 1662, 2137
BtsI GCAGTG 2 cut(s) 502, 1740
BtsIMutI CAGTG 6 cut(s) 341, 366, 502, 1109, 1740, 2365
BtuMI TCGCGA 1 cut(s) 1032
Cac8I GCNNGC 7 cut(s) 461, 536, 650, 764, 988, 1457, 1494
CaiI CAGNNNCTG 1 cut(s) 2057
CciI TCATGA 1 cut(s) 484
CfoI GCGC 7 cut(s) 236, 366, 509, 540, 654, 768, 1823
Cfr13I GGNCC 2 cut(s) 1375, 2429
ClaI ATCGAT 1 cut(s) 277
CseI GACGC 2 cut(s) 224, 629
Csp6I GTAC 4 cut(s) 492, 606, 720, 2001
CviQI GTAC 4 cut(s) 492, 606, 720, 2001
DdeI CTNAG 7 cut(s) 446, 957, 1022, 1150, 1271, 1327, 1792
DraI TTTAAA 3 cut(s) 1402, 1543, 2467
DraIII CACNNNGTG 1 cut(s) 743
EaeI YGGCCR 3 cut(s) 81, 225, 1702
Eam1104I CTCTTC 7 cut(s) 504, 618, 732, 846, 1251, 1956, 2264
EarI CTCTTC 7 cut(s) 504, 618, 732, 846, 1251, 1956, 2264
EciI GGCGGA 1 cut(s) 1756
Ecl136II GAGCTC 1 cut(s) 1040
Eco105I TACGTA 1 cut(s) 2424
Eco130I CCWWGG 1 cut(s) 1119
Eco147I AGGCCT 2 cut(s) 1285, 2247
Eco24I GRGCYC 1 cut(s) 1042
Eco31I GGTCTC 1 cut(s) 256
Eco32I GATATC 1 cut(s) 595
Eco47I GGWCC 2 cut(s) 1375, 2429
Eco53kI GAGCTC 1 cut(s) 1040
Eco57I CTGAAG 1 cut(s) 2223
Eco81I CCTNAGG 1 cut(s) 1327
EcoICRI GAGCTC 1 cut(s) 1040
EcoRI GAATTC 1 cut(s) 178
EcoRII CCWGG 2 cut(s) 693, 2056
EcoRV GATATC 1 cut(s) 595
EcoT14I CCWWGG 1 cut(s) 1119
EcoT22I ATGCAT 2 cut(s) 893, 1368
EcoT38I GRGCYC 1 cut(s) 1042
ErhI CCWWGG 1 cut(s) 1119
FalI AAGNNNNNCTT 8 cut(s) 59, 91, 563, 595, 791, 823, 810, 842
FaqI GGGAC 1 cut(s) 110
FauI CCCGC 2 cut(s) 179, 1365
FbaI TGATCA 3 cut(s) 1276, 1579, 1807
FblI GTMKAC 2 cut(s) 1231, 2406
Fnu4HI GCNGC 7 cut(s) 532, 1407, 1479, 1620, 1824, 2040, 2043
FokI GGATG 5 cut(s) 164, 1427, 1544, 1649, 2144
FriOI GRGCYC 1 cut(s) 1042
Fsp4HI GCNGC 7 cut(s) 532, 1407, 1479, 1620, 1824, 2040, 2043
FspAI RTGCGCAY 2 cut(s) 653, 767
FspBI CTAG 4 cut(s) 464, 636, 750, 1053
FspI TGCGCA 4 cut(s) 508, 539, 653, 767
GlaI GCGC 7 cut(s) 235, 365, 508, 539, 653, 767, 1822
GluI GCNGC 7 cut(s) 532, 1407, 1479, 1620, 1824, 2040, 2043
GsuI CTGGAG 2 cut(s) 390, 927
HaeIII GGCC 6 cut(s) 83, 227, 1269, 1285, 1704, 2247
HgaI GACGC 2 cut(s) 224, 629
HhaI GCGC 7 cut(s) 236, 366, 509, 540, 654, 768, 1823
Hin6I GCGC 7 cut(s) 234, 364, 507, 538, 652, 766, 1821
HinP1I GCGC 7 cut(s) 234, 364, 507, 538, 652, 766, 1821
HincII GTYRAC 2 cut(s) 1116, 2407
HindII GTYRAC 2 cut(s) 1116, 2407
HinfI GANTC 8 cut(s) 331, 413, 632, 1096, 1306, 1535, 1713, 1942
HphI GGTGA 6 cut(s) 1192, 1625, 2017, 2245, 2263, 2456
Hpy166II GTNNAC 8 cut(s) 673, 787, 1116, 1232, 1561, 1588, 2001, 2407
Hpy188I TCNGA 7 cut(s) 63, 286, 330, 336, 1526, 2131, 2203
Hpy188III TCNNGA 9 cut(s) 175, 485, 617, 636, 866, 1031, 1329, 1892, 2383
Hpy8I GTNNAC 8 cut(s) 673, 787, 1116, 1232, 1561, 1588, 2001, 2407
Hpy99I CGWCG 2 cut(s) 623, 2332
HpyAV CCTTC 4 cut(s) 142, 353, 1445, 2386
HpyCH4III ACNGT 8 cut(s) 35, 491, 552, 1113, 1172, 1777, 2005, 2053
HpyCH4IV ACGT 5 cut(s) 99, 1102, 2189, 2194, 2423
HpyF10VI GCNNNNNNNGC 7 cut(s) 167, 224, 233, 456, 531, 537, 759
HpyF3I CTNAG 7 cut(s) 446, 957, 1022, 1150, 1271, 1327, 1792
HpySE526I ACGT 5 cut(s) 99, 1102, 2189, 2194, 2423
HspAI GCGC 7 cut(s) 234, 364, 507, 538, 652, 766, 1821
Ksp22I TGATCA 3 cut(s) 1276, 1579, 1807
LguI GCTCTTC 4 cut(s) 504, 618, 732, 846
LmnI GCTCC 1 cut(s) 1037
Lsp1109I GCAGC 6 cut(s) 543, 1490, 1606, 1835, 2026, 2054
LweI GCATC 7 cut(s) 142, 184, 951, 1324, 1353, 2122, 2376
MaeI CTAG 4 cut(s) 464, 636, 750, 1053
MaeII ACGT 5 cut(s) 99, 1102, 2189, 2194, 2423
MaeIII GTNAC 5 cut(s) 1153, 1631, 1873, 2023, 2190
MbiI CCGCTC 1 cut(s) 1372
MfeI CAATTG 2 cut(s) 12, 306
MflI RGATCY 2 cut(s) 88, 1879
MhlI GDGCHC 3 cut(s) 1042, 2017, 2302
MlsI TGGCCA 1 cut(s) 1704
MluNI TGGCCA 1 cut(s) 1704
MlyI GAGTC 3 cut(s) 325, 422, 1544
MmeI TCCRAC 1 cut(s) 1504
Mox20I TGGCCA 1 cut(s) 1704
Mph1103I ATGCAT 2 cut(s) 893, 1368
MscI TGGCCA 1 cut(s) 1704
MslI CAYNNNNRTG 5 cut(s) 2030, 2234, 2276, 2279, 2384
Msp20I TGGCCA 1 cut(s) 1704
MspCI CTTAAG 1 cut(s) 1183
MspR9I CCNGG 2 cut(s) 695, 2058
MunI CAATTG 2 cut(s) 12, 306
Mva1269I GAATGC 3 cut(s) 1178, 2045, 2181
MvaI CCWGG 2 cut(s) 695, 2058
MvnI CGCG 1 cut(s) 1032
MwoI GCNNNNNNNGC 7 cut(s) 167, 224, 233, 456, 531, 537, 759
NlaIV GGNNCC 1 cut(s) 1376
NmuCI GTSAC 3 cut(s) 1631, 2023, 2190
NruI TCGCGA 1 cut(s) 1032
NsbI TGCGCA 4 cut(s) 508, 539, 653, 767
NsiI ATGCAT 2 cut(s) 893, 1368
NspI RCATGY 2 cut(s) 1496, 1609
PaeI GCATGC 1 cut(s) 1496
PagI TCATGA 1 cut(s) 484
PceI AGGCCT 2 cut(s) 1285, 2247
PciSI GCTCTTC 4 cut(s) 504, 618, 732, 846
PctI GAATGC 3 cut(s) 1178, 2045, 2181
PfeI GAWTC 5 cut(s) 632, 1096, 1306, 1713, 1942
PflFI GACNNNGTC 1 cut(s) 101
PkrI GCNGC 7 cut(s) 533, 1408, 1480, 1621, 1825, 2041, 2044
Ple19I CGATCG 1 cut(s) 438
PleI GAGTC 3 cut(s) 325, 421, 1543
PpsI GAGTC 3 cut(s) 325, 421, 1543
Ppu21I YACGTR 1 cut(s) 2424
PshBI ATTAAT 1 cut(s) 1257
PsiI TTATAA 1 cut(s) 1647
Psp124BI GAGCTC 1 cut(s) 1042
Psp1406I AACGTT 1 cut(s) 1102
Psp6I CCWGG 2 cut(s) 693, 2056
PspGI CCWGG 2 cut(s) 693, 2056
PspN4I GGNNCC 1 cut(s) 1376
PspPI GGNCC 2 cut(s) 1375, 2429
PstI CTGCAG 1 cut(s) 2044
PstNI CAGNNNCTG 1 cut(s) 2057
PsuI RGATCY 2 cut(s) 88, 1879
PsyI GACNNNGTC 1 cut(s) 101
PvuI CGATCG 1 cut(s) 438
RruI TCGCGA 1 cut(s) 1032
RsaI GTAC 4 cut(s) 493, 607, 721, 2002
RsaNI GTAC 4 cut(s) 492, 606, 720, 2001
RseI CAYNNNNRTG 5 cut(s) 2030, 2234, 2276, 2279, 2384
SacI GAGCTC 1 cut(s) 1042
SalI GTCGAC 1 cut(s) 2405
SapI GCTCTTC 4 cut(s) 504, 618, 732, 846
SatI GCNGC 7 cut(s) 532, 1407, 1479, 1620, 1824, 2040, 2043
Sau96I GGNCC 2 cut(s) 1375, 2429
SchI GAGTC 3 cut(s) 325, 422, 1544
ScrFI CCNGG 2 cut(s) 695, 2058
SduI GDGCHC 3 cut(s) 1042, 2017, 2302
SfaNI GCATC 7 cut(s) 142, 184, 951, 1324, 1353, 2122, 2376
SfcI CTRYAG 1 cut(s) 2040
SinI GGWCC 2 cut(s) 1375, 2429
SmiMI CAYNNNNRTG 5 cut(s) 2030, 2234, 2276, 2279, 2384
SmlI CTYRAG 1 cut(s) 1183
SmoI CTYRAG 1 cut(s) 1183
SnaBI TACGTA 1 cut(s) 2424
SphI GCATGC 1 cut(s) 1496
SseBI AGGCCT 2 cut(s) 1285, 2247
SsiI CCGC 5 cut(s) 186, 1056, 1372, 1407, 1741
SspMI CTAG 4 cut(s) 464, 636, 750, 1053
SstI GAGCTC 1 cut(s) 1042
StuI AGGCCT 2 cut(s) 1285, 2247
StyD4I CCNGG 2 cut(s) 693, 2056
StyI CCWWGG 1 cut(s) 1119
TaaI ACNGT 8 cut(s) 35, 491, 552, 1113, 1172, 1777, 2005, 2053
TaiI ACGT 5 cut(s) 102, 1105, 2192, 2197, 2426
TaqI TCGA 8 cut(s) 105, 174, 277, 438, 1816, 2330, 2384, 2406
TatI WGTACW 2 cut(s) 605, 2000
TauI GCSGC 1 cut(s) 1409
TfiI GAWTC 5 cut(s) 632, 1096, 1306, 1713, 1942
TscAI CASTG 6 cut(s) 348, 373, 509, 1116, 1740, 2365
TseFI GTSAC 3 cut(s) 1631, 2023, 2190
TseI GCWGC 6 cut(s) 531, 1478, 1619, 1823, 2039, 2042
Tsp45I GTSAC 3 cut(s) 1631, 2023, 2190
TspGWI ACGGA 6 cut(s) 377, 701, 1427, 1706, 1816, 2385
TspRI CASTG 6 cut(s) 348, 373, 509, 1116, 1740, 2365
Tth111I GACNNNGTC 1 cut(s) 101
Vha464I CTTAAG 1 cut(s) 1183
VpaK11BI GGWCC 2 cut(s) 1375, 2429
VspI ATTAAT 1 cut(s) 1257
XapI RAATTY 4 cut(s) 178, 477, 2090, 2468
XbaI TCTAGA 1 cut(s) 635
XceI RCATGY 2 cut(s) 1496, 1609
XmiI GTMKAC 2 cut(s) 1231, 2406
XspI CTAG 4 cut(s) 464, 636, 750, 1053
Zsp2I ATGCAT 2 cut(s) 893, 1368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.