Domain of unknown function
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 106 |
| AccB1I | GGYRCC | 1 cut(s) | 437 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 884 |
| AccI | GTMKAC | 2 cut(s) | 184, 1090 |
| AccIII | TCCGGA | 1 cut(s) | 529 |
| AciI | CCGC | 1 cut(s) | 132 |
| AclWI | GGATC | 3 cut(s) | 131, 324, 1176 |
| AcoI | YGGCCR | 1 cut(s) | 975 |
| AcsI | RAATTY | 4 cut(s) | 167, 902, 932, 970 |
| AcuI | CTGAAG | 1 cut(s) | 437 |
| AcyI | GRCGYC | 1 cut(s) | 438 |
| AdeI | CACNNNGTG | 1 cut(s) | 26 |
| AfaI | GTAC | 2 cut(s) | 68, 842 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 248, 343, 529, 580, 884 |
| AflIII | ACRYGT | 1 cut(s) | 205 |
| AgsI | TTSAA | 3 cut(s) | 41, 125, 1136 |
| AjiI | CACGTC | 2 cut(s) | 303, 1188 |
| AjnI | CCWGG | 1 cut(s) | 275 |
| AluBI | AGCT | 6 cut(s) | 200, 461, 809, 869, 1022, 1194 |
| AluI | AGCT | 6 cut(s) | 200, 461, 809, 869, 1022, 1194 |
| Alw26I | GTCTC | 3 cut(s) | 560, 760, 857 |
| AlwI | GGATC | 3 cut(s) | 131, 324, 1176 |
| Aor13HI | TCCGGA | 1 cut(s) | 529 |
| AoxI | GGCC | 4 cut(s) | 675, 895, 910, 975 |
| ApoI | RAATTY | 4 cut(s) | 167, 902, 932, 970 |
| AseI | ATTAAT | 1 cut(s) | 486 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 462 |
| AspLEI | GCGC | 3 cut(s) | 266, 440, 1032 |
| AspS9I | GGNCC | 2 cut(s) | 887, 896 |
| AsuC2I | CCSGG | 1 cut(s) | 997 |
| AsuHPI | GGTGA | 4 cut(s) | 113, 146, 263, 512 |
| AvaII | GGWCC | 1 cut(s) | 887 |
| BalI | TGGCCA | 1 cut(s) | 977 |
| BanI | GGYRCC | 1 cut(s) | 437 |
| BauI | CACGAG | 1 cut(s) | 259 |
| BbsI | GAAGAC | 1 cut(s) | 132 |
| BccI | CCATC | 4 cut(s) | 625, 772, 986, 1068 |
| BceAI | ACGGC | 4 cut(s) | 14, 360, 451, 690 |
| BciT130I | CCWGG | 1 cut(s) | 277 |
| BcnI | CCSGG | 1 cut(s) | 997 |
| BcoDI | GTCTC | 3 cut(s) | 560, 760, 857 |
| BfaI | CTAG | 2 cut(s) | 144, 806 |
| BfmI | CTRYAG | 2 cut(s) | 589, 1211 |
| BfoI | RGCGCY | 2 cut(s) | 267, 441 |
| BfuAI | ACCTGC | 1 cut(s) | 106 |
| BglII | AGATCT | 1 cut(s) | 87 |
| BmcAI | AGTACT | 1 cut(s) | 68 |
| Bme1390I | CCNGG | 2 cut(s) | 277, 997 |
| Bme18I | GGWCC | 1 cut(s) | 887 |
| BmgBI | CACGTC | 2 cut(s) | 303, 1188 |
| BmgT120I | GGNCC | 2 cut(s) | 887, 896 |
| BmiI | GGNNCC | 2 cut(s) | 439, 889 |
| BmrFI | CCNGG | 2 cut(s) | 277, 997 |
| BmrI | ACTGGG | 1 cut(s) | 885 |
| BmsI | GCATC | 1 cut(s) | 1108 |
| BmuI | ACTGGG | 1 cut(s) | 885 |
| BpiI | GAAGAC | 1 cut(s) | 132 |
| BpmI | CTGGAG | 1 cut(s) | 91 |
| BpuMI | CCSGG | 1 cut(s) | 997 |
| BsaHI | GRCGYC | 1 cut(s) | 438 |
| BsaJI | CCNNGG | 2 cut(s) | 276, 996 |
| BsaWI | WCCGGW | 2 cut(s) | 529, 862 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 64, 94 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 248, 343, 529, 580, 884 |
| Bse1I | ACTGG | 6 cut(s) | 74, 565, 766, 797, 891, 1110 |
| BseAI | TCCGGA | 1 cut(s) | 529 |
| BseBI | CCWGG | 1 cut(s) | 277 |
| BseDI | CCNNGG | 2 cut(s) | 276, 996 |
| BseGI | GGATG | 1 cut(s) | 373 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 248, 343, 529, 580, 884 |
| BseMII | CTCAG | 3 cut(s) | 748, 828, 914 |
| BseNI | ACTGG | 6 cut(s) | 74, 565, 766, 797, 891, 1110 |
| BsgI | GTGCAG | 1 cut(s) | 644 |
| BshFI | GGCC | 4 cut(s) | 677, 897, 912, 977 |
| BshNI | GGYRCC | 1 cut(s) | 437 |
| BsiSI | CCGG | 3 cut(s) | 530, 863, 996 |
| BslFI | GGGAC | 1 cut(s) | 873 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 248, 343, 529, 580, 884 |
| BsmAI | GTCTC | 3 cut(s) | 560, 760, 857 |
| BsmBI | CGTCTC | 1 cut(s) | 857 |
| BsmFI | GGGAC | 1 cut(s) | 873 |
| BsmI | GAATGC | 2 cut(s) | 196, 1037 |
| BsnI | GGCC | 4 cut(s) | 677, 897, 912, 977 |
| Bsp13I | TCCGGA | 1 cut(s) | 529 |
| Bsp143I | GATC | 4 cut(s) | 87, 136, 329, 1168 |
| BspACI | CCGC | 1 cut(s) | 132 |
| BspANI | GGCC | 4 cut(s) | 677, 897, 912, 977 |
| BspCNI | CTCAG | 3 cut(s) | 747, 829, 915 |
| BspEI | TCCGGA | 1 cut(s) | 529 |
| BspLI | GGNNCC | 2 cut(s) | 439, 889 |
| BspMAI | CTGCAG | 1 cut(s) | 593 |
| BspMI | ACCTGC | 1 cut(s) | 106 |
| BspPI | GGATC | 3 cut(s) | 131, 324, 1176 |
| BspQI | GCTCTTC | 1 cut(s) | 456 |
| BspT107I | GGYRCC | 1 cut(s) | 437 |
| BsrI | ACTGG | 6 cut(s) | 74, 565, 766, 797, 891, 1110 |
| BssECI | CCNNGG | 2 cut(s) | 276, 996 |
| BssMI | GATC | 4 cut(s) | 87, 136, 329, 1168 |
| BssNAI | GTATAC | 1 cut(s) | 1091 |
| BssNI | GRCGYC | 1 cut(s) | 438 |
| BssSI | CACGAG | 1 cut(s) | 259 |
| Bst1107I | GTATAC | 1 cut(s) | 1091 |
| Bst2BI | CACGAG | 1 cut(s) | 259 |
| Bst2UI | CCWGG | 1 cut(s) | 277 |
| Bst4CI | ACNGT | 2 cut(s) | 824, 1088 |
| Bst6I | CTCTTC | 3 cut(s) | 456, 630, 1149 |
| BstACI | GRCGYC | 1 cut(s) | 438 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 26 |
| BstC8I | GCNNGC | 4 cut(s) | 362, 731, 1024, 1028 |
| BstDEI | CTNAG | 3 cut(s) | 734, 837, 923 |
| BstF5I | GGATG | 1 cut(s) | 373 |
| BstH2I | RGCGCY | 2 cut(s) | 267, 441 |
| BstHHI | GCGC | 3 cut(s) | 266, 440, 1032 |
| BstKTI | GATC | 4 cut(s) | 90, 139, 332, 1171 |
| BstMAI | GTCTC | 3 cut(s) | 560, 760, 857 |
| BstMBI | GATC | 4 cut(s) | 87, 136, 329, 1168 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 26, 272, 446 |
| BstNI | CCWGG | 1 cut(s) | 277 |
| BstNSI | RCATGY | 2 cut(s) | 209, 1030 |
| BstSCI | CCNGG | 2 cut(s) | 275, 995 |
| BstSFI | CTRYAG | 2 cut(s) | 589, 1211 |
| BstV2I | GAAGAC | 1 cut(s) | 132 |
| BstX2I | RGATCY | 3 cut(s) | 87, 329, 1168 |
| BstYI | RGATCY | 3 cut(s) | 87, 329, 1168 |
| BstZ17I | GTATAC | 1 cut(s) | 1091 |
| BsuRI | GGCC | 4 cut(s) | 677, 897, 912, 977 |
| BtrI | CACGTC | 2 cut(s) | 303, 1188 |
| BtsCI | GGATG | 1 cut(s) | 373 |
| BtsIMutI | CAGTG | 5 cut(s) | 318, 759, 898, 1084, 1188 |
| BveI | ACCTGC | 1 cut(s) | 106 |
| Cac8I | GCNNGC | 4 cut(s) | 362, 731, 1024, 1028 |
| CfoI | GCGC | 3 cut(s) | 266, 440, 1032 |
| Cfr13I | GGNCC | 2 cut(s) | 887, 896 |
| Csp6I | GTAC | 2 cut(s) | 67, 841 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 748, 783 |
| CviAII | CATG | 6 cut(s) | 52, 206, 425, 467, 580, 1027 |
| CviQI | GTAC | 2 cut(s) | 67, 841 |
| DdeI | CTNAG | 3 cut(s) | 734, 837, 923 |
| DinI | GGCGCC | 1 cut(s) | 439 |
| DpnI | GATC | 4 cut(s) | 89, 138, 331, 1170 |
| DpnII | GATC | 4 cut(s) | 87, 136, 329, 1168 |
| DraI | TTTAAA | 1 cut(s) | 1011 |
| DraIII | CACNNNGTG | 1 cut(s) | 26 |
| EaeI | YGGCCR | 1 cut(s) | 975 |
| Eam1104I | CTCTTC | 3 cut(s) | 456, 630, 1149 |
| EarI | CTCTTC | 3 cut(s) | 456, 630, 1149 |
| Eco47I | GGWCC | 1 cut(s) | 887 |
| Eco57I | CTGAAG | 1 cut(s) | 437 |
| EcoRII | CCWGG | 1 cut(s) | 275 |
| EgeI | GGCGCC | 1 cut(s) | 439 |
| EheI | GGCGCC | 1 cut(s) | 439 |
| Esp3I | CGTCTC | 1 cut(s) | 857 |
| FaeI | CATG | 6 cut(s) | 55, 209, 428, 470, 583, 1030 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 77, 109 |
| FaqI | GGGAC | 1 cut(s) | 873 |
| FatI | CATG | 6 cut(s) | 51, 205, 424, 466, 579, 1026 |
| FblI | GTMKAC | 2 cut(s) | 184, 1090 |
| FokI | GGATG | 1 cut(s) | 380 |
| FspBI | CTAG | 2 cut(s) | 144, 806 |
| GlaI | GCGC | 3 cut(s) | 265, 439, 1031 |
| GsuI | CTGGAG | 1 cut(s) | 91 |
| HaeII | RGCGCY | 2 cut(s) | 267, 441 |
| HaeIII | GGCC | 4 cut(s) | 677, 897, 912, 977 |
| HapII | CCGG | 3 cut(s) | 530, 863, 996 |
| HhaI | GCGC | 3 cut(s) | 266, 440, 1032 |
| Hin1I | GRCGYC | 1 cut(s) | 438 |
| Hin1II | CATG | 6 cut(s) | 55, 209, 428, 470, 583, 1030 |
| Hin6I | GCGC | 3 cut(s) | 264, 438, 1030 |
| HinP1I | GCGC | 3 cut(s) | 264, 438, 1030 |
| HincII | GTYRAC | 1 cut(s) | 724 |
| HindII | GTYRAC | 1 cut(s) | 724 |
| HindIII | AAGCTT | 2 cut(s) | 198, 459 |
| HinfI | GANTC | 3 cut(s) | 73, 1159, 1178 |
| HpaII | CCGG | 3 cut(s) | 530, 863, 996 |
| HphI | GGTGA | 4 cut(s) | 113, 146, 263, 512 |
| Hpy166II | GTNNAC | 4 cut(s) | 185, 300, 724, 1091 |
| Hpy188I | TCNGA | 5 cut(s) | 307, 334, 737, 924, 1173 |
| Hpy188III | TCNNGA | 4 cut(s) | 38, 455, 530, 596 |
| Hpy8I | GTNNAC | 4 cut(s) | 185, 300, 724, 1091 |
| Hpy99I | CGWCG | 1 cut(s) | 482 |
| HpyAV | CCTTC | 3 cut(s) | 347, 584, 659 |
| HpyCH4III | ACNGT | 2 cut(s) | 824, 1088 |
| HpyCH4IV | ACGT | 3 cut(s) | 302, 375, 1187 |
| HpyCH4V | TGCA | 6 cut(s) | 20, 194, 591, 625, 944, 1037 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 26, 272, 446 |
| HpyF3I | CTNAG | 3 cut(s) | 734, 837, 923 |
| HpySE526I | ACGT | 3 cut(s) | 302, 375, 1187 |
| Hsp92I | GRCGYC | 1 cut(s) | 438 |
| Hsp92II | CATG | 6 cut(s) | 55, 209, 428, 470, 583, 1030 |
| HspAI | GCGC | 3 cut(s) | 264, 438, 1030 |
| KasI | GGCGCC | 1 cut(s) | 437 |
| Kpn2I | TCCGGA | 1 cut(s) | 529 |
| Kzo9I | GATC | 4 cut(s) | 87, 136, 329, 1168 |
| LguI | GCTCTTC | 1 cut(s) | 456 |
| LmnI | GCTCC | 1 cut(s) | 1191 |
| LweI | GCATC | 1 cut(s) | 1108 |
| MaeI | CTAG | 2 cut(s) | 144, 806 |
| MaeII | ACGT | 3 cut(s) | 302, 375, 1187 |
| MaeIII | GTNAC | 2 cut(s) | 251, 1183 |
| MalI | GATC | 4 cut(s) | 89, 138, 331, 1170 |
| MboI | GATC | 4 cut(s) | 87, 136, 329, 1168 |
| MfeI | CAATTG | 1 cut(s) | 15 |
| MflI | RGATCY | 3 cut(s) | 87, 329, 1168 |
| MlsI | TGGCCA | 1 cut(s) | 977 |
| MluCI | AATT | 9 cut(s) | 15, 167, 210, 792, 902, 932, 954, 970, 1146 |
| MluNI | TGGCCA | 1 cut(s) | 977 |
| Mly113I | GGCGCC | 1 cut(s) | 438 |
| MlyI | GAGTC | 2 cut(s) | 82, 1187 |
| MmeI | TCCRAC | 1 cut(s) | 357 |
| MnlI | CCTC | 7 cut(s) | 97, 150, 598, 607, 854, 923, 1093 |
| Mox20I | TGGCCA | 1 cut(s) | 977 |
| MroI | TCCGGA | 1 cut(s) | 529 |
| MroXI | GAANNNNTTC | 1 cut(s) | 462 |
| MscI | TGGCCA | 1 cut(s) | 977 |
| MseI | TTAA | 4 cut(s) | 84, 486, 953, 1010 |
| Msp20I | TGGCCA | 1 cut(s) | 977 |
| MspA1I | CMGCKG | 1 cut(s) | 132 |
| MspI | CCGG | 3 cut(s) | 530, 863, 996 |
| MspR9I | CCNGG | 2 cut(s) | 277, 997 |
| MunI | CAATTG | 1 cut(s) | 15 |
| Mva1269I | GAATGC | 2 cut(s) | 196, 1037 |
| MvaI | CCWGG | 1 cut(s) | 277 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 26, 272, 446 |
| NarI | GGCGCC | 1 cut(s) | 438 |
| NciI | CCSGG | 1 cut(s) | 997 |
| NdeII | GATC | 4 cut(s) | 87, 136, 329, 1168 |
| NlaIII | CATG | 6 cut(s) | 55, 209, 428, 470, 583, 1030 |
| NlaIV | GGNNCC | 2 cut(s) | 439, 889 |
| NmuCI | GTSAC | 2 cut(s) | 251, 1183 |
| NspI | RCATGY | 2 cut(s) | 209, 1030 |
| PaeI | GCATGC | 1 cut(s) | 1030 |
| PciI | ACATGT | 1 cut(s) | 205 |
| PciSI | GCTCTTC | 1 cut(s) | 456 |
| PctI | GAATGC | 2 cut(s) | 196, 1037 |
| PdmI | GAANNNNTTC | 1 cut(s) | 462 |
| PfeI | GAWTC | 1 cut(s) | 1159 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 884 |
| PleI | GAGTC | 2 cut(s) | 81, 1186 |
| PluTI | GGCGCC | 1 cut(s) | 441 |
| PpsI | GAGTC | 2 cut(s) | 81, 1186 |
| PscI | ACATGT | 1 cut(s) | 205 |
| PshBI | ATTAAT | 1 cut(s) | 486 |
| Psp6I | CCWGG | 1 cut(s) | 275 |
| PspGI | CCWGG | 1 cut(s) | 275 |
| PspN4I | GGNNCC | 2 cut(s) | 439, 889 |
| PspPI | GGNCC | 2 cut(s) | 887, 896 |
| PstI | CTGCAG | 1 cut(s) | 593 |
| PsuI | RGATCY | 3 cut(s) | 87, 329, 1168 |
| RsaI | GTAC | 2 cut(s) | 68, 842 |
| RsaNI | GTAC | 2 cut(s) | 67, 841 |
| SapI | GCTCTTC | 1 cut(s) | 456 |
| SaqAI | TTAA | 4 cut(s) | 84, 486, 953, 1010 |
| Sau3AI | GATC | 4 cut(s) | 87, 136, 329, 1168 |
| Sau96I | GGNCC | 2 cut(s) | 887, 896 |
| ScaI | AGTACT | 1 cut(s) | 68 |
| SchI | GAGTC | 2 cut(s) | 82, 1187 |
| ScrFI | CCNGG | 2 cut(s) | 277, 997 |
| SfaNI | GCATC | 1 cut(s) | 1108 |
| SfcI | CTRYAG | 2 cut(s) | 589, 1211 |
| SfoI | GGCGCC | 1 cut(s) | 439 |
| SinI | GGWCC | 1 cut(s) | 887 |
| SmlI | CTYRAG | 1 cut(s) | 1195 |
| SmoI | CTYRAG | 1 cut(s) | 1195 |
| SphI | GCATGC | 1 cut(s) | 1030 |
| Sse9I | AATT | 9 cut(s) | 15, 167, 210, 792, 902, 932, 954, 970, 1146 |
| SsiI | CCGC | 1 cut(s) | 132 |
| SspDI | GGCGCC | 1 cut(s) | 437 |
| SspMI | CTAG | 2 cut(s) | 144, 806 |
| StyD4I | CCNGG | 2 cut(s) | 275, 995 |
| TaaI | ACNGT | 2 cut(s) | 824, 1088 |
| TaiI | ACGT | 3 cut(s) | 305, 378, 1190 |
| TaqI | TCGA | 2 cut(s) | 477, 553 |
| TasI | AATT | 9 cut(s) | 15, 167, 210, 792, 902, 932, 954, 970, 1146 |
| TatI | WGTACW | 1 cut(s) | 66 |
| TfiI | GAWTC | 1 cut(s) | 1159 |
| Tru1I | TTAA | 4 cut(s) | 84, 486, 953, 1010 |
| Tru9I | TTAA | 4 cut(s) | 84, 486, 953, 1010 |
| TscAI | CASTG | 5 cut(s) | 325, 766, 898, 1091, 1188 |
| TseFI | GTSAC | 2 cut(s) | 251, 1183 |
| Tsp45I | GTSAC | 2 cut(s) | 251, 1183 |
| TspDTI | ATGAA | 3 cut(s) | 384, 455, 1167 |
| TspRI | CASTG | 5 cut(s) | 325, 766, 898, 1091, 1188 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 884 |
| VpaK11BI | GGWCC | 1 cut(s) | 887 |
| VspI | ATTAAT | 1 cut(s) | 486 |
| XapI | RAATTY | 4 cut(s) | 167, 902, 932, 970 |
| XceI | RCATGY | 2 cut(s) | 209, 1030 |
| XmiI | GTMKAC | 2 cut(s) | 184, 1090 |
| XmnI | GAANNNNTTC | 1 cut(s) | 462 |
| XspI | CTAG | 2 cut(s) | 144, 806 |
| ZrmI | AGTACT | 1 cut(s) | 68 |