Domain of unknown function
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 332 |
| AclWI | GGATC | 1 cut(s) | 557 |
| AcoI | YGGCCR | 3 cut(s) | 283, 315, 492 |
| AcsI | RAATTY | 2 cut(s) | 404, 533 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 53 |
| AgsI | TTSAA | 3 cut(s) | 86, 227, 431 |
| AjiI | CACGTC | 1 cut(s) | 94 |
| AjnI | CCWGG | 1 cut(s) | 363 |
| AluBI | AGCT | 1 cut(s) | 350 |
| AluI | AGCT | 1 cut(s) | 350 |
| Alw26I | GTCTC | 1 cut(s) | 144 |
| AlwI | GGATC | 1 cut(s) | 557 |
| AoxI | GGCC | 4 cut(s) | 283, 315, 447, 492 |
| ApoI | RAATTY | 2 cut(s) | 404, 533 |
| AspLEI | GCGC | 1 cut(s) | 371 |
| AspS9I | GGNCC | 1 cut(s) | 448 |
| BalI | TGGCCA | 1 cut(s) | 285 |
| BccI | CCATC | 1 cut(s) | 55 |
| BceAI | ACGGC | 1 cut(s) | 183 |
| BciT130I | CCWGG | 1 cut(s) | 365 |
| BcoDI | GTCTC | 1 cut(s) | 144 |
| BfaI | CTAG | 1 cut(s) | 401 |
| BglII | AGATCT | 1 cut(s) | 442 |
| Bme1390I | CCNGG | 1 cut(s) | 365 |
| BmgBI | CACGTC | 1 cut(s) | 94 |
| BmgT120I | GGNCC | 1 cut(s) | 448 |
| BmrFI | CCNGG | 1 cut(s) | 365 |
| BmsI | GCATC | 1 cut(s) | 246 |
| BpmI | CTGGAG | 1 cut(s) | 324 |
| BsaJI | CCNNGG | 2 cut(s) | 195, 364 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 53 |
| Bse118I | RCCGGY | 1 cut(s) | 494 |
| Bse1I | ACTGG | 1 cut(s) | 278 |
| BseBI | CCWGG | 1 cut(s) | 365 |
| BseDI | CCNNGG | 2 cut(s) | 195, 364 |
| BseGI | GGATG | 2 cut(s) | 201, 261 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 53 |
| BseNI | ACTGG | 1 cut(s) | 278 |
| BsgI | GTGCAG | 1 cut(s) | 137 |
| BshFI | GGCC | 4 cut(s) | 285, 317, 449, 494 |
| BsiSI | CCGG | 1 cut(s) | 495 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 53 |
| BsmAI | GTCTC | 1 cut(s) | 144 |
| BsmBI | CGTCTC | 1 cut(s) | 144 |
| BsnI | GGCC | 4 cut(s) | 285, 317, 449, 494 |
| Bsp143I | GATC | 2 cut(s) | 442, 549 |
| BspACI | CCGC | 1 cut(s) | 332 |
| BspANI | GGCC | 4 cut(s) | 285, 317, 449, 494 |
| BspPI | GGATC | 1 cut(s) | 557 |
| BsrFI | RCCGGY | 1 cut(s) | 494 |
| BsrI | ACTGG | 1 cut(s) | 278 |
| BssAI | RCCGGY | 1 cut(s) | 494 |
| BssECI | CCNNGG | 2 cut(s) | 195, 364 |
| BssMI | GATC | 2 cut(s) | 442, 549 |
| BssT1I | CCWWGG | 1 cut(s) | 195 |
| Bst2UI | CCWGG | 1 cut(s) | 365 |
| Bst4CI | ACNGT | 2 cut(s) | 161, 467 |
| BstDEI | CTNAG | 1 cut(s) | 245 |
| BstF5I | GGATG | 2 cut(s) | 201, 261 |
| BstHHI | GCGC | 1 cut(s) | 371 |
| BstKTI | GATC | 2 cut(s) | 445, 552 |
| BstMAI | GTCTC | 1 cut(s) | 144 |
| BstMBI | GATC | 2 cut(s) | 442, 549 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 366 |
| BstNI | CCWGG | 1 cut(s) | 365 |
| BstNSI | RCATGY | 1 cut(s) | 253 |
| BstSCI | CCNGG | 1 cut(s) | 363 |
| BstX2I | RGATCY | 2 cut(s) | 442, 549 |
| BstYI | RGATCY | 2 cut(s) | 442, 549 |
| BsuRI | GGCC | 4 cut(s) | 285, 317, 449, 494 |
| BtrI | CACGTC | 1 cut(s) | 94 |
| BtsCI | GGATG | 2 cut(s) | 201, 261 |
| BtsI | GCAGTG | 1 cut(s) | 385 |
| BtsIMutI | CAGTG | 1 cut(s) | 385 |
| CfoI | GCGC | 1 cut(s) | 371 |
| Cfr10I | RCCGGY | 1 cut(s) | 494 |
| Cfr13I | GGNCC | 1 cut(s) | 448 |
| CseI | GACGC | 2 cut(s) | 161, 349 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 374, 409, 451, 486 |
| CviAII | CATG | 3 cut(s) | 58, 73, 250 |
| CviJI | RGCY | 7 cut(s) | 170, 182, 285, 317, 350, 449, 494 |
| CviKI_1 | RGCY | 7 cut(s) | 170, 182, 285, 317, 350, 449, 494 |
| DdeI | CTNAG | 1 cut(s) | 245 |
| DpnI | GATC | 2 cut(s) | 444, 551 |
| DpnII | GATC | 2 cut(s) | 442, 549 |
| EaeI | YGGCCR | 3 cut(s) | 283, 315, 492 |
| Eco130I | CCWWGG | 1 cut(s) | 195 |
| EcoRII | CCWGG | 1 cut(s) | 363 |
| EcoT14I | CCWWGG | 1 cut(s) | 195 |
| ErhI | CCWWGG | 1 cut(s) | 195 |
| Esp3I | CGTCTC | 1 cut(s) | 144 |
| FaeI | CATG | 3 cut(s) | 61, 76, 253 |
| FaiI | YATR | 4 cut(s) | 59, 74, 251, 555 |
| FatI | CATG | 3 cut(s) | 57, 72, 249 |
| FokI | GGATG | 2 cut(s) | 188, 268 |
| FspBI | CTAG | 1 cut(s) | 401 |
| GlaI | GCGC | 1 cut(s) | 370 |
| GsuI | CTGGAG | 1 cut(s) | 324 |
| HaeIII | GGCC | 4 cut(s) | 285, 317, 449, 494 |
| HapII | CCGG | 1 cut(s) | 495 |
| HgaI | GACGC | 2 cut(s) | 161, 349 |
| HhaI | GCGC | 1 cut(s) | 371 |
| Hin1II | CATG | 3 cut(s) | 61, 76, 253 |
| Hin6I | GCGC | 1 cut(s) | 369 |
| HinP1I | GCGC | 1 cut(s) | 369 |
| HincII | GTYRAC | 1 cut(s) | 527 |
| HindII | GTYRAC | 1 cut(s) | 527 |
| HinfI | GANTC | 2 cut(s) | 238, 274 |
| HpaII | CCGG | 1 cut(s) | 495 |
| Hpy166II | GTNNAC | 1 cut(s) | 527 |
| Hpy188I | TCNGA | 1 cut(s) | 188 |
| Hpy188III | TCNNGA | 3 cut(s) | 86, 341, 440 |
| Hpy8I | GTNNAC | 1 cut(s) | 527 |
| HpyAV | CCTTC | 1 cut(s) | 92 |
| HpyCH4III | ACNGT | 2 cut(s) | 161, 467 |
| HpyCH4IV | ACGT | 1 cut(s) | 93 |
| HpyCH4V | TGCA | 3 cut(s) | 118, 176, 509 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 366 |
| HpyF3I | CTNAG | 1 cut(s) | 245 |
| HpySE526I | ACGT | 1 cut(s) | 93 |
| Hsp92II | CATG | 3 cut(s) | 61, 76, 253 |
| HspAI | GCGC | 1 cut(s) | 369 |
| Kzo9I | GATC | 2 cut(s) | 442, 549 |
| LweI | GCATC | 1 cut(s) | 246 |
| MaeI | CTAG | 1 cut(s) | 401 |
| MaeII | ACGT | 1 cut(s) | 93 |
| MalI | GATC | 2 cut(s) | 444, 551 |
| MboI | GATC | 2 cut(s) | 442, 549 |
| MboII | GAAGA | 2 cut(s) | 59, 201 |
| MfeI | CAATTG | 1 cut(s) | 488 |
| MflI | RGATCY | 2 cut(s) | 442, 549 |
| MlsI | TGGCCA | 1 cut(s) | 285 |
| MluCI | AATT | 8 cut(s) | 63, 110, 304, 374, 404, 426, 488, 533 |
| MluNI | TGGCCA | 1 cut(s) | 285 |
| MmeI | TCCRAC | 1 cut(s) | 166 |
| Mox20I | TGGCCA | 1 cut(s) | 285 |
| MscI | TGGCCA | 1 cut(s) | 285 |
| MseI | TTAA | 5 cut(s) | 29, 425, 482, 515, 531 |
| MslI | CAYNNNNRTG | 1 cut(s) | 254 |
| Msp20I | TGGCCA | 1 cut(s) | 285 |
| MspI | CCGG | 1 cut(s) | 495 |
| MspR9I | CCNGG | 1 cut(s) | 365 |
| MunI | CAATTG | 1 cut(s) | 488 |
| MvaI | CCWGG | 1 cut(s) | 365 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 366 |
| NdeII | GATC | 2 cut(s) | 442, 549 |
| NlaIII | CATG | 3 cut(s) | 61, 76, 253 |
| NspI | RCATGY | 1 cut(s) | 253 |
| PfeI | GAWTC | 2 cut(s) | 238, 274 |
| Psp6I | CCWGG | 1 cut(s) | 363 |
| PspGI | CCWGG | 1 cut(s) | 363 |
| PspPI | GGNCC | 1 cut(s) | 448 |
| PsuI | RGATCY | 2 cut(s) | 442, 549 |
| RseI | CAYNNNNRTG | 1 cut(s) | 254 |
| SaqAI | TTAA | 5 cut(s) | 29, 425, 482, 515, 531 |
| Sau3AI | GATC | 2 cut(s) | 442, 549 |
| Sau96I | GGNCC | 1 cut(s) | 448 |
| ScrFI | CCNGG | 1 cut(s) | 365 |
| SetI | ASST | 2 cut(s) | 96, 352 |
| SfaNI | GCATC | 1 cut(s) | 246 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 254 |
| Sse9I | AATT | 8 cut(s) | 63, 110, 304, 374, 404, 426, 488, 533 |
| SsiI | CCGC | 1 cut(s) | 332 |
| SspI | AATATT | 1 cut(s) | 100 |
| SspMI | CTAG | 1 cut(s) | 401 |
| StyD4I | CCNGG | 1 cut(s) | 363 |
| StyI | CCWWGG | 1 cut(s) | 195 |
| TaaI | ACNGT | 2 cut(s) | 161, 467 |
| TaiI | ACGT | 1 cut(s) | 96 |
| TasI | AATT | 8 cut(s) | 63, 110, 304, 374, 404, 426, 488, 533 |
| TfiI | GAWTC | 2 cut(s) | 238, 274 |
| Tru1I | TTAA | 5 cut(s) | 29, 425, 482, 515, 531 |
| Tru9I | TTAA | 5 cut(s) | 29, 425, 482, 515, 531 |
| TscAI | CASTG | 1 cut(s) | 392 |
| TspDTI | ATGAA | 2 cut(s) | 123, 140 |
| TspRI | CASTG | 1 cut(s) | 392 |
| XapI | RAATTY | 2 cut(s) | 404, 533 |
| XceI | RCATGY | 1 cut(s) | 253 |
| XspI | CTAG | 1 cut(s) | 401 |