Domain of unknown function
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 786 |
| Acc36I | ACCTGC | 1 cut(s) | 814 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 152 |
| AccII | CGCG | 1 cut(s) | 673 |
| AciI | CCGC | 4 cut(s) | 129, 323, 407, 671 |
| AcoI | YGGCCR | 1 cut(s) | 678 |
| AcsI | RAATTY | 4 cut(s) | 270, 465, 495, 635 |
| AcyI | GRCGYC | 1 cut(s) | 319 |
| AfaI | GTAC | 1 cut(s) | 365 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 152, 281, 587, 676 |
| AflII | CTTAAG | 1 cut(s) | 914 |
| AflIII | ACRYGT | 1 cut(s) | 999 |
| AgsI | TTSAA | 4 cut(s) | 38, 489, 532, 629 |
| AjnI | CCWGG | 1 cut(s) | 647 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 135, 167 |
| AluBI | AGCT | 8 cut(s) | 8, 200, 293, 398, 404, 512, 760, 922 |
| AluI | AGCT | 8 cut(s) | 8, 200, 293, 398, 404, 512, 760, 922 |
| Alw21I | GWGCWC | 1 cut(s) | 348 |
| Alw26I | GTCTC | 2 cut(s) | 578, 1001 |
| Alw44I | GTGCAC | 1 cut(s) | 344 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 768 |
| AoxI | GGCC | 2 cut(s) | 589, 678 |
| ApaLI | GTGCAC | 1 cut(s) | 344 |
| ApeKI | GCWGC | 5 cut(s) | 224, 404, 410, 772, 818 |
| ApoI | RAATTY | 4 cut(s) | 270, 465, 495, 635 |
| AspLEI | GCGC | 2 cut(s) | 229, 675 |
| AspS9I | GGNCC | 1 cut(s) | 590 |
| AsuHPI | GGTGA | 2 cut(s) | 143, 224 |
| BaeGI | GKGCMC | 1 cut(s) | 348 |
| BalI | TGGCCA | 1 cut(s) | 680 |
| Bbv12I | GWGCWC | 1 cut(s) | 348 |
| BbvI | GCAGC | 5 cut(s) | 236, 391, 397, 784, 830 |
| BccI | CCATC | 4 cut(s) | 203, 380, 742, 771 |
| BciT130I | CCWGG | 1 cut(s) | 649 |
| BcoDI | GTCTC | 2 cut(s) | 578, 1001 |
| BfaI | CTAG | 4 cut(s) | 401, 509, 687, 981 |
| BfmI | CTRYAG | 1 cut(s) | 9 |
| BfrI | CTTAAG | 1 cut(s) | 914 |
| BfuAI | ACCTGC | 1 cut(s) | 814 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 597 |
| BisI | GCNGC | 6 cut(s) | 225, 405, 408, 411, 773, 819 |
| BlsI | GCNGC | 6 cut(s) | 226, 406, 409, 412, 774, 820 |
| BmcAI | AGTACT | 1 cut(s) | 365 |
| Bme1390I | CCNGG | 1 cut(s) | 649 |
| BmgT120I | GGNCC | 1 cut(s) | 590 |
| BmrFI | CCNGG | 1 cut(s) | 649 |
| BmsI | GCATC | 5 cut(s) | 151, 244, 256, 301, 342 |
| BsaHI | GRCGYC | 1 cut(s) | 319 |
| BsaJI | CCNNGG | 2 cut(s) | 563, 648 |
| BsaWI | WCCGGW | 1 cut(s) | 725 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 152, 281, 587, 676 |
| Bse118I | RCCGGY | 1 cut(s) | 125 |
| Bse3DI | GCAATG | 1 cut(s) | 300 |
| BseBI | CCWGG | 1 cut(s) | 649 |
| BseDI | CCNNGG | 2 cut(s) | 563, 648 |
| BseGI | GGATG | 2 cut(s) | 214, 247 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 152, 281, 587, 676 |
| BseMI | GCAATG | 1 cut(s) | 300 |
| BseMII | CTCAG | 3 cut(s) | 737, 775, 940 |
| BseRI | GAGGAG | 1 cut(s) | 393 |
| BseSI | GKGCMC | 1 cut(s) | 348 |
| BseXI | GCAGC | 5 cut(s) | 236, 391, 397, 784, 830 |
| Bsh1236I | CGCG | 1 cut(s) | 673 |
| BshFI | GGCC | 2 cut(s) | 591, 680 |
| BsiHKAI | GWGCWC | 1 cut(s) | 348 |
| BsiSI | CCGG | 2 cut(s) | 126, 726 |
| BslFI | GGGAC | 1 cut(s) | 125 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 152, 281, 587, 676 |
| BsmAI | GTCTC | 2 cut(s) | 578, 1001 |
| BsmFI | GGGAC | 1 cut(s) | 125 |
| BsmI | GAATGC | 1 cut(s) | 740 |
| BsnI | GGCC | 2 cut(s) | 591, 680 |
| Bsp1286I | GDGCHC | 1 cut(s) | 348 |
| Bsp143I | GATC | 3 cut(s) | 103, 691, 977 |
| BspACI | CCGC | 4 cut(s) | 129, 323, 407, 671 |
| BspANI | GGCC | 2 cut(s) | 591, 680 |
| BspCNI | CTCAG | 3 cut(s) | 738, 774, 939 |
| BspFNI | CGCG | 1 cut(s) | 673 |
| BspHI | TCATGA | 1 cut(s) | 100 |
| BspMI | ACCTGC | 1 cut(s) | 814 |
| BspQI | GCTCTTC | 1 cut(s) | 300 |
| BspTI | CTTAAG | 1 cut(s) | 914 |
| BsrDI | GCAATG | 1 cut(s) | 300 |
| BsrFI | RCCGGY | 1 cut(s) | 125 |
| BssAI | RCCGGY | 1 cut(s) | 125 |
| BssECI | CCNNGG | 2 cut(s) | 563, 648 |
| BssMI | GATC | 3 cut(s) | 103, 691, 977 |
| BssNI | GRCGYC | 1 cut(s) | 319 |
| Bst2UI | CCWGG | 1 cut(s) | 649 |
| Bst4CI | ACNGT | 4 cut(s) | 13, 339, 554, 791 |
| Bst6I | CTCTTC | 3 cut(s) | 300, 354, 868 |
| BstACI | GRCGYC | 1 cut(s) | 319 |
| BstAFI | CTTAAG | 1 cut(s) | 914 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 235, 311, 352 |
| BstC8I | GCNNGC | 3 cut(s) | 166, 323, 541 |
| BstDEI | CTNAG | 4 cut(s) | 470, 746, 761, 926 |
| BstDSI | CCRYGG | 1 cut(s) | 563 |
| BstEII | GGTNACC | 1 cut(s) | 131 |
| BstF5I | GGATG | 2 cut(s) | 214, 247 |
| BstFNI | CGCG | 1 cut(s) | 673 |
| BstHHI | GCGC | 2 cut(s) | 229, 675 |
| BstKTI | GATC | 3 cut(s) | 106, 694, 980 |
| BstMAI | GTCTC | 2 cut(s) | 578, 1001 |
| BstMBI | GATC | 3 cut(s) | 103, 691, 977 |
| BstNI | CCWGG | 1 cut(s) | 649 |
| BstNSI | RCATGY | 1 cut(s) | 1003 |
| BstPI | GGTNACC | 1 cut(s) | 131 |
| BstSCI | CCNGG | 1 cut(s) | 647 |
| BstSFI | CTRYAG | 1 cut(s) | 9 |
| BstSLI | GKGCMC | 1 cut(s) | 348 |
| BstUI | CGCG | 1 cut(s) | 673 |
| BstV1I | GCAGC | 5 cut(s) | 236, 391, 397, 784, 830 |
| BsuRI | GGCC | 2 cut(s) | 591, 680 |
| BtgI | CCRYGG | 1 cut(s) | 563 |
| BtgZI | GCGATG | 1 cut(s) | 790 |
| BtsCI | GGATG | 2 cut(s) | 214, 247 |
| BveI | ACCTGC | 1 cut(s) | 814 |
| Cac8I | GCNNGC | 3 cut(s) | 166, 323, 541 |
| CaiI | CAGNNNCTG | 1 cut(s) | 768 |
| CciI | TCATGA | 1 cut(s) | 100 |
| CfoI | GCGC | 2 cut(s) | 229, 675 |
| Cfr10I | RCCGGY | 1 cut(s) | 125 |
| Cfr13I | GGNCC | 1 cut(s) | 590 |
| CseI | GACGC | 2 cut(s) | 327, 491 |
| Csp6I | GTAC | 1 cut(s) | 364 |
| CviAII | CATG | 6 cut(s) | 101, 391, 445, 520, 958, 1000 |
| CviQI | GTAC | 1 cut(s) | 364 |
| DdeI | CTNAG | 4 cut(s) | 470, 746, 761, 926 |
| DpnI | GATC | 3 cut(s) | 105, 693, 979 |
| DpnII | GATC | 3 cut(s) | 103, 691, 977 |
| DraI | TTTAAA | 1 cut(s) | 196 |
| EaeI | YGGCCR | 1 cut(s) | 678 |
| Eam1104I | CTCTTC | 3 cut(s) | 300, 354, 868 |
| EarI | CTCTTC | 3 cut(s) | 300, 354, 868 |
| Eco91I | GGTNACC | 1 cut(s) | 131 |
| EcoO65I | GGTNACC | 1 cut(s) | 131 |
| EcoRI | GAATTC | 3 cut(s) | 270, 465, 495 |
| EcoRII | CCWGG | 1 cut(s) | 647 |
| EcoT22I | ATGCAT | 1 cut(s) | 446 |
| FaeI | CATG | 6 cut(s) | 104, 394, 448, 523, 961, 1003 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 222, 254 |
| FaqI | GGGAC | 1 cut(s) | 125 |
| FatI | CATG | 6 cut(s) | 100, 390, 444, 519, 957, 999 |
| FauI | CCCGC | 2 cut(s) | 330, 678 |
| Fnu4HI | GCNGC | 6 cut(s) | 225, 405, 408, 411, 773, 819 |
| FokI | GGATG | 2 cut(s) | 221, 234 |
| Fsp4HI | GCNGC | 6 cut(s) | 225, 405, 408, 411, 773, 819 |
| FspBI | CTAG | 4 cut(s) | 401, 509, 687, 981 |
| GlaI | GCGC | 2 cut(s) | 228, 674 |
| GluI | GCNGC | 6 cut(s) | 225, 405, 408, 411, 773, 819 |
| HaeIII | GGCC | 2 cut(s) | 591, 680 |
| HapII | CCGG | 2 cut(s) | 126, 726 |
| HgaI | GACGC | 2 cut(s) | 327, 491 |
| HhaI | GCGC | 2 cut(s) | 229, 675 |
| Hin1I | GRCGYC | 1 cut(s) | 319 |
| Hin1II | CATG | 6 cut(s) | 104, 394, 448, 523, 961, 1003 |
| Hin6I | GCGC | 2 cut(s) | 227, 673 |
| HinP1I | GCGC | 2 cut(s) | 227, 673 |
| HindIII | AAGCTT | 1 cut(s) | 920 |
| HinfI | GANTC | 3 cut(s) | 423, 765, 967 |
| HpaII | CCGG | 2 cut(s) | 126, 726 |
| HphI | GGTGA | 2 cut(s) | 143, 224 |
| Hpy166II | GTNNAC | 3 cut(s) | 346, 997, 1027 |
| Hpy188I | TCNGA | 3 cut(s) | 160, 616, 764 |
| Hpy188III | TCNNGA | 4 cut(s) | 101, 251, 687, 745 |
| Hpy8I | GTNNAC | 3 cut(s) | 346, 997, 1027 |
| Hpy99I | CGWCG | 2 cut(s) | 179, 485 |
| HpyAV | CCTTC | 1 cut(s) | 857 |
| HpyCH4III | ACNGT | 4 cut(s) | 13, 339, 554, 791 |
| HpyCH4V | TGCA | 6 cut(s) | 26, 257, 305, 346, 413, 444 |
| HpyF3I | CTNAG | 4 cut(s) | 470, 746, 761, 926 |
| Hsp92I | GRCGYC | 1 cut(s) | 319 |
| Hsp92II | CATG | 6 cut(s) | 104, 394, 448, 523, 961, 1003 |
| HspAI | GCGC | 2 cut(s) | 227, 673 |
| Kzo9I | GATC | 3 cut(s) | 103, 691, 977 |
| LguI | GCTCTTC | 1 cut(s) | 300 |
| LmnI | GCTCC | 1 cut(s) | 814 |
| Lsp1109I | GCAGC | 5 cut(s) | 236, 391, 397, 784, 830 |
| LweI | GCATC | 5 cut(s) | 151, 244, 256, 301, 342 |
| MaeI | CTAG | 4 cut(s) | 401, 509, 687, 981 |
| MaeIII | GTNAC | 2 cut(s) | 131, 556 |
| MalI | GATC | 3 cut(s) | 105, 693, 979 |
| MboI | GATC | 3 cut(s) | 103, 691, 977 |
| MboII | GAAGA | 6 cut(s) | 74, 287, 371, 466, 581, 885 |
| MfeI | CAATTG | 1 cut(s) | 606 |
| MhlI | GDGCHC | 1 cut(s) | 348 |
| MlsI | TGGCCA | 1 cut(s) | 680 |
| MluCI | AATT | 6 cut(s) | 270, 465, 495, 606, 635, 657 |
| MluNI | TGGCCA | 1 cut(s) | 680 |
| MnlI | CCTC | 3 cut(s) | 146, 199, 371 |
| Mox20I | TGGCCA | 1 cut(s) | 680 |
| Mph1103I | ATGCAT | 1 cut(s) | 446 |
| MscI | TGGCCA | 1 cut(s) | 680 |
| MseI | TTAA | 5 cut(s) | 81, 195, 575, 656, 915 |
| MslI | CAYNNNNRTG | 2 cut(s) | 252, 431 |
| Msp20I | TGGCCA | 1 cut(s) | 680 |
| MspCI | CTTAAG | 1 cut(s) | 914 |
| MspI | CCGG | 2 cut(s) | 126, 726 |
| MspR9I | CCNGG | 1 cut(s) | 649 |
| MunI | CAATTG | 1 cut(s) | 606 |
| Mva1269I | GAATGC | 1 cut(s) | 740 |
| MvaI | CCWGG | 1 cut(s) | 649 |
| MvnI | CGCG | 1 cut(s) | 673 |
| NdeII | GATC | 3 cut(s) | 103, 691, 977 |
| NlaIII | CATG | 6 cut(s) | 104, 394, 448, 523, 961, 1003 |
| NmuCI | GTSAC | 2 cut(s) | 131, 556 |
| NsiI | ATGCAT | 1 cut(s) | 446 |
| NspI | RCATGY | 1 cut(s) | 1003 |
| PagI | TCATGA | 1 cut(s) | 100 |
| PciI | ACATGT | 1 cut(s) | 999 |
| PciSI | GCTCTTC | 1 cut(s) | 300 |
| PctI | GAATGC | 1 cut(s) | 740 |
| PfeI | GAWTC | 3 cut(s) | 423, 765, 967 |
| PflFI | GACNNNGTC | 1 cut(s) | 116 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 152 |
| PkrI | GCNGC | 6 cut(s) | 226, 406, 409, 412, 774, 820 |
| PscI | ACATGT | 1 cut(s) | 999 |
| PsiI | TTATAA | 1 cut(s) | 786 |
| Psp6I | CCWGG | 1 cut(s) | 647 |
| PspEI | GGTNACC | 1 cut(s) | 131 |
| PspGI | CCWGG | 1 cut(s) | 647 |
| PspPI | GGNCC | 1 cut(s) | 590 |
| PstNI | CAGNNNCTG | 1 cut(s) | 768 |
| PsyI | GACNNNGTC | 1 cut(s) | 116 |
| RsaI | GTAC | 1 cut(s) | 365 |
| RsaNI | GTAC | 1 cut(s) | 364 |
| RseI | CAYNNNNRTG | 2 cut(s) | 252, 431 |
| SapI | GCTCTTC | 1 cut(s) | 300 |
| SaqAI | TTAA | 5 cut(s) | 81, 195, 575, 656, 915 |
| SatI | GCNGC | 6 cut(s) | 225, 405, 408, 411, 773, 819 |
| Sau3AI | GATC | 3 cut(s) | 103, 691, 977 |
| Sau96I | GGNCC | 1 cut(s) | 590 |
| ScaI | AGTACT | 1 cut(s) | 365 |
| ScrFI | CCNGG | 1 cut(s) | 649 |
| SduI | GDGCHC | 1 cut(s) | 348 |
| SfaNI | GCATC | 5 cut(s) | 151, 244, 256, 301, 342 |
| SfcI | CTRYAG | 1 cut(s) | 9 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 252, 431 |
| SmlI | CTYRAG | 1 cut(s) | 914 |
| SmoI | CTYRAG | 1 cut(s) | 914 |
| Sse9I | AATT | 6 cut(s) | 270, 465, 495, 606, 635, 657 |
| SsiI | CCGC | 4 cut(s) | 129, 323, 407, 671 |
| SspMI | CTAG | 4 cut(s) | 401, 509, 687, 981 |
| StyD4I | CCNGG | 1 cut(s) | 647 |
| TaaI | ACNGT | 4 cut(s) | 13, 339, 554, 791 |
| TaqI | TCGA | 4 cut(s) | 120, 174, 430, 871 |
| TasI | AATT | 6 cut(s) | 270, 465, 495, 606, 635, 657 |
| TatI | WGTACW | 1 cut(s) | 363 |
| TauI | GCSGC | 1 cut(s) | 410 |
| TfiI | GAWTC | 3 cut(s) | 423, 765, 967 |
| Tru1I | TTAA | 5 cut(s) | 81, 195, 575, 656, 915 |
| Tru9I | TTAA | 5 cut(s) | 81, 195, 575, 656, 915 |
| TseFI | GTSAC | 2 cut(s) | 131, 556 |
| TseI | GCWGC | 5 cut(s) | 224, 404, 410, 772, 818 |
| Tsp45I | GTSAC | 2 cut(s) | 131, 556 |
| TspDTI | ATGAA | 5 cut(s) | 198, 613, 770, 959, 974 |
| TspGWI | ACGGA | 1 cut(s) | 967 |
| Tth111I | GACNNNGTC | 1 cut(s) | 116 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 152 |
| Vha464I | CTTAAG | 1 cut(s) | 914 |
| VneI | GTGCAC | 1 cut(s) | 344 |
| XapI | RAATTY | 4 cut(s) | 270, 465, 495, 635 |
| XbaI | TCTAGA | 1 cut(s) | 686 |
| XceI | RCATGY | 1 cut(s) | 1003 |
| XspI | CTAG | 4 cut(s) | 401, 509, 687, 981 |
| ZrmI | AGTACT | 1 cut(s) | 365 |
| Zsp2I | ATGCAT | 1 cut(s) | 446 |