ankyrin repeat family protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 396 |
| AatII | GACGTC | 1 cut(s) | 830 |
| AccI | GTMKAC | 2 cut(s) | 830, 1048 |
| AccII | CGCG | 1 cut(s) | 379 |
| AciI | CCGC | 4 cut(s) | 164, 225, 379, 771 |
| AclWI | GGATC | 5 cut(s) | 739, 1043, 1079, 1140, 1222 |
| AcoI | YGGCCR | 3 cut(s) | 264, 306, 772 |
| AcsI | RAATTY | 1 cut(s) | 167 |
| AcuI | CTGAAG | 1 cut(s) | 123 |
| AcyI | GRCGYC | 1 cut(s) | 827 |
| AfaI | GTAC | 1 cut(s) | 1214 |
| AflIII | ACRYGT | 1 cut(s) | 1010 |
| AgeI | ACCGGT | 1 cut(s) | 368 |
| AgsI | TTSAA | 8 cut(s) | 21, 137, 301, 415, 561, 1097, 1238, 1247 |
| AjiI | CACGTC | 1 cut(s) | 1013 |
| AjnI | CCWGG | 1 cut(s) | 275 |
| AluBI | AGCT | 8 cut(s) | 261, 361, 401, 542, 647, 653, 659, 882 |
| AluI | AGCT | 8 cut(s) | 261, 361, 401, 542, 647, 653, 659, 882 |
| Alw26I | GTCTC | 3 cut(s) | 122, 1019, 1180 |
| AlwI | GGATC | 5 cut(s) | 739, 1043, 1079, 1140, 1222 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1023 |
| AoxI | GGCC | 4 cut(s) | 264, 306, 772, 933 |
| ApoI | RAATTY | 1 cut(s) | 167 |
| AsiGI | ACCGGT | 1 cut(s) | 368 |
| AspS9I | GGNCC | 1 cut(s) | 671 |
| AsuC2I | CCSGG | 2 cut(s) | 251, 1115 |
| AsuHPI | GGTGA | 3 cut(s) | 170, 473, 1199 |
| AvaII | GGWCC | 1 cut(s) | 671 |
| BanII | GRGCYC | 1 cut(s) | 1173 |
| BbsI | GAAGAC | 2 cut(s) | 618, 633 |
| BccI | CCATC | 3 cut(s) | 149, 340, 438 |
| BceAI | ACGGC | 1 cut(s) | 421 |
| BciT130I | CCWGG | 1 cut(s) | 277 |
| BciVI | GTATCC | 1 cut(s) | 274 |
| BclI | TGATCA | 2 cut(s) | 753, 1123 |
| BcnI | CCSGG | 2 cut(s) | 251, 1115 |
| BcoDI | GTCTC | 3 cut(s) | 122, 1019, 1180 |
| BfaI | CTAG | 2 cut(s) | 144, 684 |
| BfmI | CTRYAG | 1 cut(s) | 648 |
| BfuI | GTATCC | 1 cut(s) | 274 |
| BglII | AGATCT | 1 cut(s) | 106 |
| BisI | GCNGC | 1 cut(s) | 772 |
| BlsI | GCNGC | 1 cut(s) | 773 |
| Bme1390I | CCNGG | 3 cut(s) | 251, 277, 1115 |
| Bme18I | GGWCC | 1 cut(s) | 671 |
| BmgBI | CACGTC | 1 cut(s) | 1013 |
| BmgT120I | GGNCC | 1 cut(s) | 671 |
| BmiI | GGNNCC | 1 cut(s) | 673 |
| BmrFI | CCNGG | 3 cut(s) | 251, 277, 1115 |
| BmrI | ACTGGG | 2 cut(s) | 787, 963 |
| BmsI | GCATC | 3 cut(s) | 436, 505, 677 |
| BmuI | ACTGGG | 2 cut(s) | 787, 963 |
| BpiI | GAAGAC | 2 cut(s) | 618, 633 |
| BpmI | CTGGAG | 2 cut(s) | 487, 719 |
| BpuMI | CCSGG | 2 cut(s) | 251, 1115 |
| Bsa29I | ATCGAT | 2 cut(s) | 1074, 1203 |
| BsaHI | GRCGYC | 1 cut(s) | 827 |
| BsaI | GGTCTC | 1 cut(s) | 122 |
| BsaJI | CCNNGG | 4 cut(s) | 195, 250, 276, 318 |
| BsaWI | WCCGGW | 1 cut(s) | 368 |
| Bse118I | RCCGGY | 2 cut(s) | 304, 368 |
| Bse1I | ACTGG | 5 cut(s) | 470, 782, 856, 897, 958 |
| BseBI | CCWGG | 1 cut(s) | 277 |
| BseCI | ATCGAT | 2 cut(s) | 1074, 1203 |
| BseDI | CCNNGG | 4 cut(s) | 195, 250, 276, 318 |
| BseGI | GGATG | 4 cut(s) | 235, 340, 496, 606 |
| BseMII | CTCAG | 2 cut(s) | 1030, 1161 |
| BseNI | ACTGG | 5 cut(s) | 470, 782, 856, 897, 958 |
| BseRI | GAGGAG | 1 cut(s) | 401 |
| BseX3I | CGGCCG | 1 cut(s) | 306 |
| Bsh1236I | CGCG | 1 cut(s) | 379 |
| Bsh1285I | CGRYCG | 1 cut(s) | 309 |
| BshFI | GGCC | 4 cut(s) | 266, 308, 774, 935 |
| BshTI | ACCGGT | 1 cut(s) | 368 |
| BshVI | ATCGAT | 2 cut(s) | 1074, 1203 |
| BsiEI | CGRYCG | 1 cut(s) | 309 |
| BsiSI | CCGG | 4 cut(s) | 250, 305, 369, 1115 |
| BsmAI | GTCTC | 3 cut(s) | 122, 1019, 1180 |
| BsmBI | CGTCTC | 2 cut(s) | 1019, 1180 |
| BsnI | GGCC | 4 cut(s) | 266, 308, 774, 935 |
| Bso31I | GGTCTC | 1 cut(s) | 122 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1173 |
| Bsp1407I | TGTACA | 1 cut(s) | 1212 |
| BspACI | CCGC | 4 cut(s) | 164, 225, 379, 771 |
| BspANI | GGCC | 4 cut(s) | 266, 308, 774, 935 |
| BspCNI | CTCAG | 2 cut(s) | 1029, 1160 |
| BspDI | ATCGAT | 2 cut(s) | 1074, 1203 |
| BspFNI | CGCG | 1 cut(s) | 379 |
| BspHI | TCATGA | 1 cut(s) | 52 |
| BspLI | GGNNCC | 1 cut(s) | 673 |
| BspPI | GGATC | 5 cut(s) | 739, 1043, 1079, 1140, 1222 |
| BspQI | GCTCTTC | 2 cut(s) | 351, 549 |
| BspTNI | GGTCTC | 1 cut(s) | 122 |
| BsrFI | RCCGGY | 2 cut(s) | 304, 368 |
| BsrGI | TGTACA | 1 cut(s) | 1212 |
| BsrI | ACTGG | 5 cut(s) | 470, 782, 856, 897, 958 |
| BssAI | RCCGGY | 2 cut(s) | 304, 368 |
| BssECI | CCNNGG | 4 cut(s) | 195, 250, 276, 318 |
| BssNAI | GTATAC | 1 cut(s) | 1049 |
| BssNI | GRCGYC | 1 cut(s) | 827 |
| BssT1I | CCWWGG | 2 cut(s) | 195, 318 |
| Bst1107I | GTATAC | 1 cut(s) | 1049 |
| Bst2UI | CCWGG | 1 cut(s) | 277 |
| Bst4CI | ACNGT | 5 cut(s) | 13, 845, 875, 1046, 1183 |
| Bst6I | CTCTTC | 3 cut(s) | 120, 351, 549 |
| BstACI | GRCGYC | 1 cut(s) | 827 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 726 |
| BstAUI | TGTACA | 1 cut(s) | 1212 |
| BstC8I | GCNNGC | 2 cut(s) | 306, 790 |
| BstDEI | CTNAG | 3 cut(s) | 47, 1016, 1147 |
| BstF5I | GGATG | 4 cut(s) | 235, 340, 496, 606 |
| BstFNI | CGCG | 1 cut(s) | 379 |
| BstMAI | GTCTC | 3 cut(s) | 122, 1019, 1180 |
| BstMCI | CGRYCG | 1 cut(s) | 309 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 272, 314, 726 |
| BstNI | CCWGG | 1 cut(s) | 277 |
| BstNSI | RCATGY | 3 cut(s) | 209, 1064, 1264 |
| BstSCI | CCNGG | 3 cut(s) | 249, 275, 1113 |
| BstSFI | CTRYAG | 1 cut(s) | 648 |
| BstUI | CGCG | 1 cut(s) | 379 |
| BstV2I | GAAGAC | 2 cut(s) | 618, 633 |
| BstX2I | RGATCY | 4 cut(s) | 106, 1035, 1132, 1227 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 892 |
| BstYI | RGATCY | 4 cut(s) | 106, 1035, 1132, 1227 |
| BstZ17I | GTATAC | 1 cut(s) | 1049 |
| BstZI | CGGCCG | 1 cut(s) | 306 |
| Bsu15I | ATCGAT | 2 cut(s) | 1074, 1203 |
| BsuI | GTATCC | 1 cut(s) | 274 |
| BsuRI | GGCC | 4 cut(s) | 266, 308, 774, 935 |
| BsuTUI | ATCGAT | 2 cut(s) | 1074, 1203 |
| BtrI | CACGTC | 1 cut(s) | 1013 |
| BtsCI | GGATG | 4 cut(s) | 235, 340, 496, 606 |
| BtsIMutI | CAGTG | 3 cut(s) | 749, 775, 871 |
| Cac8I | GCNNGC | 2 cut(s) | 306, 790 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1023 |
| CciI | TCATGA | 1 cut(s) | 52 |
| Cfr10I | RCCGGY | 2 cut(s) | 304, 368 |
| Cfr13I | GGNCC | 1 cut(s) | 671 |
| ClaI | ATCGAT | 2 cut(s) | 1074, 1203 |
| CseI | GACGC | 3 cut(s) | 385, 512, 638 |
| Csp6I | GTAC | 1 cut(s) | 1213 |
| CspAI | ACCGGT | 1 cut(s) | 368 |
| CviAII | CATG | 9 cut(s) | 53, 206, 353, 550, 757, 767, 1061, 1232, 1261 |
| CviQI | GTAC | 1 cut(s) | 1213 |
| DdeI | CTNAG | 3 cut(s) | 47, 1016, 1147 |
| EaeI | YGGCCR | 3 cut(s) | 264, 306, 772 |
| EagI | CGGCCG | 1 cut(s) | 306 |
| Eam1104I | CTCTTC | 3 cut(s) | 120, 351, 549 |
| EarI | CTCTTC | 3 cut(s) | 120, 351, 549 |
| EclXI | CGGCCG | 1 cut(s) | 306 |
| Eco130I | CCWWGG | 2 cut(s) | 195, 318 |
| Eco24I | GRGCYC | 1 cut(s) | 1173 |
| Eco31I | GGTCTC | 1 cut(s) | 122 |
| Eco47I | GGWCC | 1 cut(s) | 671 |
| Eco52I | CGGCCG | 1 cut(s) | 306 |
| Eco57I | CTGAAG | 1 cut(s) | 123 |
| EcoO109I | RGGNCCY | 1 cut(s) | 671 |
| EcoRII | CCWGG | 1 cut(s) | 275 |
| EcoT14I | CCWWGG | 2 cut(s) | 195, 318 |
| EcoT22I | ATGCAT | 2 cut(s) | 498, 692 |
| EcoT38I | GRGCYC | 1 cut(s) | 1173 |
| ErhI | CCWWGG | 2 cut(s) | 195, 318 |
| Esp3I | CGTCTC | 2 cut(s) | 1019, 1180 |
| FaeI | CATG | 9 cut(s) | 56, 209, 356, 553, 760, 770, 1064, 1235, 1264 |
| FatI | CATG | 9 cut(s) | 52, 205, 352, 549, 756, 766, 1060, 1231, 1260 |
| FauI | CCCGC | 1 cut(s) | 218 |
| FbaI | TGATCA | 2 cut(s) | 753, 1123 |
| FblI | GTMKAC | 2 cut(s) | 830, 1048 |
| Fnu4HI | GCNGC | 1 cut(s) | 772 |
| FokI | GGATG | 4 cut(s) | 242, 347, 483, 613 |
| FriOI | GRGCYC | 1 cut(s) | 1173 |
| Fsp4HI | GCNGC | 1 cut(s) | 772 |
| FspBI | CTAG | 2 cut(s) | 144, 684 |
| GluI | GCNGC | 1 cut(s) | 772 |
| GsuI | CTGGAG | 2 cut(s) | 487, 719 |
| HaeIII | GGCC | 4 cut(s) | 266, 308, 774, 935 |
| HapII | CCGG | 4 cut(s) | 250, 305, 369, 1115 |
| HgaI | GACGC | 3 cut(s) | 385, 512, 638 |
| Hin1I | GRCGYC | 1 cut(s) | 827 |
| Hin1II | CATG | 9 cut(s) | 56, 209, 356, 553, 760, 770, 1064, 1235, 1264 |
| HincII | GTYRAC | 1 cut(s) | 831 |
| HindII | GTYRAC | 1 cut(s) | 831 |
| HinfI | GANTC | 3 cut(s) | 270, 1020, 1200 |
| HpaII | CCGG | 4 cut(s) | 250, 305, 369, 1115 |
| HphI | GGTGA | 3 cut(s) | 170, 473, 1199 |
| Hpy166II | GTNNAC | 4 cut(s) | 524, 831, 1049, 1190 |
| Hpy188I | TCNGA | 5 cut(s) | 375, 512, 849, 1019, 1199 |
| Hpy188III | TCNNGA | 4 cut(s) | 53, 500, 697, 736 |
| Hpy8I | GTNNAC | 4 cut(s) | 524, 831, 1049, 1190 |
| Hpy99I | CGWCG | 2 cut(s) | 506, 832 |
| HpyAV | CCTTC | 1 cut(s) | 1271 |
| HpyCH4III | ACNGT | 5 cut(s) | 13, 845, 875, 1046, 1183 |
| HpyCH4IV | ACGT | 2 cut(s) | 827, 1012 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 272, 314, 726 |
| HpyF3I | CTNAG | 3 cut(s) | 47, 1016, 1147 |
| HpySE526I | ACGT | 2 cut(s) | 827, 1012 |
| Hsp92I | GRCGYC | 1 cut(s) | 827 |
| Hsp92II | CATG | 9 cut(s) | 56, 209, 356, 553, 760, 770, 1064, 1235, 1264 |
| KroI | GCCGGC | 1 cut(s) | 304 |
| KroNI | GCCGGC | 1 cut(s) | 306 |
| Ksp22I | TGATCA | 2 cut(s) | 753, 1123 |
| LguI | GCTCTTC | 2 cut(s) | 351, 549 |
| LmnI | GCTCC | 1 cut(s) | 887 |
| LweI | GCATC | 3 cut(s) | 436, 505, 677 |
| MaeI | CTAG | 2 cut(s) | 144, 684 |
| MaeII | ACGT | 2 cut(s) | 827, 1012 |
| MaeIII | GTNAC | 4 cut(s) | 7, 461, 640, 805 |
| MflI | RGATCY | 4 cut(s) | 106, 1035, 1132, 1227 |
| MhlI | GDGCHC | 1 cut(s) | 1173 |
| MluCI | AATT | 6 cut(s) | 27, 58, 167, 912, 974, 1140 |
| MmeI | TCCRAC | 1 cut(s) | 827 |
| MnlI | CCTC | 9 cut(s) | 109, 150, 174, 325, 379, 512, 601, 1000, 1160 |
| Mph1103I | ATGCAT | 2 cut(s) | 498, 692 |
| MroNI | GCCGGC | 1 cut(s) | 304 |
| MseI | TTAA | 6 cut(s) | 39, 84, 911, 1001, 1119, 1290 |
| MspI | CCGG | 4 cut(s) | 250, 305, 369, 1115 |
| MspR9I | CCNGG | 3 cut(s) | 251, 277, 1115 |
| MvaI | CCWGG | 1 cut(s) | 277 |
| MvnI | CGCG | 1 cut(s) | 379 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 272, 314, 726 |
| NaeI | GCCGGC | 1 cut(s) | 306 |
| NciI | CCSGG | 2 cut(s) | 251, 1115 |
| NgoMIV | GCCGGC | 1 cut(s) | 304 |
| NlaIII | CATG | 9 cut(s) | 56, 209, 356, 553, 760, 770, 1064, 1235, 1264 |
| NlaIV | GGNNCC | 1 cut(s) | 673 |
| NmeAIII | GCCGAG | 1 cut(s) | 242 |
| NmuCI | GTSAC | 2 cut(s) | 461, 640 |
| NsiI | ATGCAT | 2 cut(s) | 498, 692 |
| NspI | RCATGY | 3 cut(s) | 209, 1064, 1264 |
| PagI | TCATGA | 1 cut(s) | 52 |
| PciSI | GCTCTTC | 2 cut(s) | 351, 549 |
| PdiI | GCCGGC | 1 cut(s) | 306 |
| PfeI | GAWTC | 3 cut(s) | 270, 1020, 1200 |
| PinAI | ACCGGT | 1 cut(s) | 368 |
| PkrI | GCNGC | 1 cut(s) | 773 |
| PpuMI | RGGWCCY | 1 cut(s) | 671 |
| PsiI | TTATAA | 1 cut(s) | 396 |
| Psp5II | RGGWCCY | 1 cut(s) | 671 |
| Psp6I | CCWGG | 1 cut(s) | 275 |
| PspGI | CCWGG | 1 cut(s) | 275 |
| PspN4I | GGNNCC | 1 cut(s) | 673 |
| PspPI | GGNCC | 1 cut(s) | 671 |
| PspPPI | RGGWCCY | 1 cut(s) | 671 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1023 |
| PsuI | RGATCY | 4 cut(s) | 106, 1035, 1132, 1227 |
| RsaI | GTAC | 1 cut(s) | 1214 |
| RsaNI | GTAC | 1 cut(s) | 1213 |
| SalI | GTCGAC | 1 cut(s) | 829 |
| SapI | GCTCTTC | 2 cut(s) | 351, 549 |
| SaqAI | TTAA | 6 cut(s) | 39, 84, 911, 1001, 1119, 1290 |
| SatI | GCNGC | 1 cut(s) | 772 |
| Sau96I | GGNCC | 1 cut(s) | 671 |
| ScrFI | CCNGG | 3 cut(s) | 251, 277, 1115 |
| SduI | GDGCHC | 1 cut(s) | 1173 |
| SfaNI | GCATC | 3 cut(s) | 436, 505, 677 |
| SfcI | CTRYAG | 1 cut(s) | 648 |
| SinI | GGWCC | 1 cut(s) | 671 |
| Sse9I | AATT | 6 cut(s) | 27, 58, 167, 912, 974, 1140 |
| SsiI | CCGC | 4 cut(s) | 164, 225, 379, 771 |
| SspMI | CTAG | 2 cut(s) | 144, 684 |
| StyD4I | CCNGG | 3 cut(s) | 249, 275, 1113 |
| StyI | CCWWGG | 2 cut(s) | 195, 318 |
| TaaI | ACNGT | 5 cut(s) | 13, 845, 875, 1046, 1183 |
| TaiI | ACGT | 2 cut(s) | 830, 1015 |
| TaqI | TCGA | 4 cut(s) | 696, 830, 1074, 1203 |
| TasI | AATT | 6 cut(s) | 27, 58, 167, 912, 974, 1140 |
| TatI | WGTACW | 1 cut(s) | 1212 |
| TauI | GCSGC | 1 cut(s) | 774 |
| TfiI | GAWTC | 3 cut(s) | 270, 1020, 1200 |
| Tru1I | TTAA | 6 cut(s) | 39, 84, 911, 1001, 1119, 1290 |
| Tru9I | TTAA | 6 cut(s) | 39, 84, 911, 1001, 1119, 1290 |
| TscAI | CASTG | 3 cut(s) | 756, 782, 878 |
| TseFI | GTSAC | 2 cut(s) | 461, 640 |
| Tsp45I | GTSAC | 2 cut(s) | 461, 640 |
| TspDTI | ATGAA | 3 cut(s) | 78, 369, 1235 |
| TspGWI | ACGGA | 2 cut(s) | 1175, 1300 |
| TspRI | CASTG | 3 cut(s) | 756, 782, 878 |
| VpaK11BI | GGWCC | 1 cut(s) | 671 |
| XapI | RAATTY | 1 cut(s) | 167 |
| XceI | RCATGY | 3 cut(s) | 209, 1064, 1264 |
| XmiI | GTMKAC | 2 cut(s) | 830, 1048 |
| XspI | CTAG | 2 cut(s) | 144, 684 |
| ZraI | GACGTC | 1 cut(s) | 828 |
| Zsp2I | ATGCAT | 2 cut(s) | 498, 692 |