Rh7BG408300

protein ubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0000027
Physical Location & Seq
Forward (+)
45859732 .. 45896749
37018 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG408300.1

Sequence Viewer

Length: 2379 bp
ATGGCAAACGCTACAGTTGTCTCCAATGCAATTGTCCCTGAAGTTCTCAACCAGCATAATTACAAGGATTGGAGTTCTCGAGTTAAGACGTACTTGTTGGCTGAAGATTTGTGGGATATGGTCGAAACGGAACCTCCTGGATCAGAAGAAAAGGATTGGGAAAAGAAGAATGCCAAGGCTTTACATACAATTAAAATTTCTTGCGGGCCTGAACTGTATACTTTCATTAGGGAATCTTCCACAGCCAAGATTGCCTGGGAAGTCTTGGAGAAACAGTTCATATCACGAAAAATTTTACGTTTACTGAAGAAAGAGAGAGAAGACAAAAAGACATCTTGTCACCCTGACGAGGTGGAAATAAAAGGGTGTACTGAAGGAAATGAGAATAAAGCCAACTACGACTTCGACTCCATCTACAACTCTTTCTTTGATTATGTGTGCAATGGTGATTGGGAGAATGCACAGGAGTGCATAACAGAACTGAATCCCCACGATGCAGTAAGAGCTAGAGATCCAAGAAACGGATCCACAGCTCTTCACTTGGCAGCCAGGAAAGGGCATCTGCATATTGTGAAAGAGTTGCTGGTATTGATGACACCAGAAGATTTGAAAACAACAGACAAGGACGGTTACACAGCTCTTCACATAGCAGTTTTGAACGGGCATGTGGATATTGTGAAAGAGGTGGTGCTCTTGATGAGTCAAGAAGCTTTGGAAAATAAAGACAATGCTGGTTATACTGCTTTAGGTTGTGCTTTCCATGTAGACAGAAACCACAAAGAGGAAGACGTGATTGAGATGGCTGAATACATGGTTGAAAAGAACAGAAGAATACTTGGCATCGCCCTTCCTGCACACCATTATATTCCAGTTGTGGAGGCATCTAGTTCAATCGACAAATGGAAAGTGGCCCGGTATCTGTATTCCGTCACTCCAGTTGAATATCTAATTCCGCCGGAGAACGGTTCCCAAGGTGCTACACTAATTTGCTGCTGCATTAAAACAAATGAATTGGATATTGCATTGGACTTGCTAAAAAAATGCCCAAGCCTGGCTATCGTTAAAACGGTTTCTGGAAGAGAATTTCCAATTTTGGTATTGGTTCGGATGCATTCTACCTTTTTAAGAGGATTACAACTCAACTTTTGGAAAAAATGGATTTATAACGGTATACGAATACAAGCCCATACAAGTTTTAACATTCATGAGATTTCTATAGATATTTTCAAATCAGAATATGATGAAGGTAATCAAAGGGATCTCGTTATTACAGATTCAAAATTATTCAGAAGACTTGCTGAGGGGTTGCTTCAACTTTTCGGAGTACTAAATCGTATCTATGAAATGAAATCAACCCATGGCCAAATCTTCGAGCTTATCAGTTGCATGGGTGAAGAGACAAGAAATAATATGAACTCTTTTACAGTAGAACAACTCAACATGGTGAACGCAGCTTTATTCGAAGCTATTGAACAGGGGAACATTGAGTTTGTTACTCATGTGTGTAAAGAAAATTCAAGACTCATCGACACATTTGATGAAAAAAGGAGGAGTATATTTCATTTTGCCATTGAATGTCGTCAAGAAGAGATCTATAGTCTTATATATGGTCTTGAAAAACAAAAGATGGATGAATTTGGACTTCATTTAGCCAACTTTAGAAATAGTATGCTGCACAGTGCAGCAAATTTATCTCCAGTTTCAAAGTTCAATCAAATTCAAGGTGCATATTTACAAATGCAAAGAGAACTACAGTGGTTTCAGGAGGTGGAGAATGTTGTACCTCTAGCAATGCATGATTCTAGAAATAGTTCAGATGATTTGACAGCACGTGAACTATTTTTCAAGAATCACAAGAAATTGATGGAGGATGCAGAAATATCAATGAAAGGGATAGCAGCTTCTTCTACAGTTGTAGGTGCTCTCATTGTTACCATCATGTTTGCAGCAGCATTCACAATCCCTGGCGGAAATAATGGTTCCACAGGCCTTCCCATGTTCCTAGAAGAGAACTTGTTTCTGGTTTTTATAGTGTCTGATACTATATCACTCGTTTCTTCTACAACTTCAGTCATTATATTTTTGGGAATTCTCACGTCACGATACGCACAAGATGATTACCTCAAAAATTTGCCCACAAAGATGATGATAGGGCTATTTACCCTTTTTATCGCTATCGCCACAATGATGACTGCATTTTGTTGTGGCCTTGTCATTATGCTTCATGGCAAATATTCATGGGTTGCTATTCCAAGCATTCTGGTTGCTAGTATTCCGGTCACCTCATTTGTTTGGATGCTGTTTCCTCTCCTTCTTCAGACTTTCATCTCTACTTATGGACCTGGAATATTCAACAGGAAAGTCAAACAGTGGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

792

Amino Acids

90.06

Weight (kDa)

5.83

Isoelectric Point (pI)

41.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4219 PF13961 16 - 42 2.1e-07 Domain of unknown function (DUF4219)
Retrotran_gag_2 PF14223 29 - 97 1.7e-07 gag-polypeptide of LTR copia-type
Ank_2 PF12796 156 - 228 8.4e-14 Ankyrin repeats (3 copies)
Ank_5 PF13857 171 - 214 2.8e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 200 - 258 6.1e-07 Ankyrin repeats (3 copies)
Ank_5 PF13857 200 - 249 1.4e-06 Ankyrin repeats (many copies)
PGG PF13962 633 - 739 6.8e-22 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000027)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18670 AT3G18670 AT3G54065
fragaria_vesca FvH4_1g26570 FvH4_1g26570 FvH4_5g19270 FvH4_5g19273 FvH4_5g35840 FvH4_5g36340 FvH4_5g36630 FvH4_5g37181 FvH4_5g37182 FvH4_5g37185 FvH4_5g37240 FvH4_5g37260 FvH4_6g21440 FvH4_6g44571 FvH4_6g44572 FvH4_6g44610 FvH4_6g44620 FvH4_6g44650 FvH4_6g44660 FvH4_6g45151 FvH4_6g45152 FvH4_6g45152 FvH4_6g45153 FvH4_6g45153 FvH4_6g45153 FvH4_6g45153 FvH4_6g45153 FvH4_6g45154 FvH4_6g51440 FvH4_7g08090 FvH4_7g08090 FvH4_7g08090 FvH4_7g08090 FvH4_7g08090 FvH4_7g08090
malus_domestica MD00G1010300.v1.1 MD00G1010400.v1.1 MD00G1010500.v1.1 MD00G1011000.v1.1 MD00G1011200.v1.1 MD00G1011500.v1.1 MD00G1011900.v1.1 MD03G1133900.v1.1 MD03G1134100.v1.1 MD09G1086300.v1.1 MD09G1086500.v1.1 MD09G1086900.v1.1 MD09G1087000.v1.1 MD09G1088000.v1.1 MD09G1088100.v1.1 MD09G1088200.v1.1 MD09G1088300.v1.1 MD09G1089000.v1.1 MD09G1089300.v1.1 MD09G1133800.v1.1 MD16G1219400.v1.1 MD17G1027900.v1.1 MD17G1028400.v1.1 MD17G1028600.v1.1 MD17G1028700.v1.1 MD17G1028900.v1.1 MD17G1029000.v1.1 MD17G1029100.v1.1 MD17G1029200.v1.1 MD17G1075400.v1.1 MD17G1076000.v1.1 MD17G1077000.v1.1 MD17G1077100.v1.1 MD17G1077800.v1.1 MD17G1078000.v1.1 MD17G1078100.v1.1 MD17G1078300.v1.1 MD17G1100900.v1.1 MD17G1101100.v1.1 MD17G1101400.v1.1 MD17G1104200.v1.1 MD17G1104500.v1.1 MD17G1121300.v1.1 MD17G1121600.v1.1 MD17G1121900.v1.1 MD17G1122700.v1.1 MD17G1218800.v1.1
prunus_persica Prupe.2G033800_v2.0.a1 Prupe.2G033900_v2.0.a1 Prupe.2G080300_v2.0.a1 Prupe.2G080700_v2.0.a1 Prupe.2G080900_v2.0.a1 Prupe.2G081100_v2.0.a1 Prupe.2G081100_v2.0.a1 Prupe.2G081200_v2.0.a1 Prupe.2G081200_v2.0.a1 Prupe.2G081200_v2.0.a1 Prupe.2G081200_v2.0.a1 Prupe.2G081700_v2.0.a1 Prupe.2G081700_v2.0.a1 Prupe.2G082000_v2.0.a1 Prupe.2G082100_v2.0.a1 Prupe.2G082100_v2.0.a1 Prupe.2G082200_v2.0.a1 Prupe.2G082300_v2.0.a1 Prupe.2G082700_v2.0.a1 Prupe.2G082800_v2.0.a1 Prupe.2G082900_v2.0.a1 Prupe.3G235600_v2.0.a1 Prupe.3G236300_v2.0.a1 Prupe.3G236300_v2.0.a1 Prupe.3G236600_v2.0.a1 Prupe.3G236600_v2.0.a1 Prupe.3G236800_v2.0.a1 Prupe.3G237000_v2.0.a1 Prupe.3G237100_v2.0.a1 Prupe.3G237300_v2.0.a1 Prupe.3G237400_v2.0.a1 Prupe.3G237500_v2.0.a1 Prupe.3G237500_v2.0.a1 Prupe.3G237500_v2.0.a1 Prupe.4G282500_v2.0.a1 Prupe.4G282500_v2.0.a1
pyrus_communis pycom03g08990 pycom03g09000 pycom09g01180 pycom09g01190 pycom09g01220 pycom09g01230 pycom09g01360 pycom09g01370 pycom09g01380 pycom09g01410 pycom09g05500 pycom11g10280 pycom11g10290 pycom11g10310 pycom16g18270 pycom16g18280 pycom17g02280 pycom17g02290 pycom17g02300 pycom17g02360 pycom17g02390 pycom17g02410 pycom17g02440 pycom17g02460 pycom17g02470 pycom17g02480 pycom17g02510 pycom17g02530 pycom17g02540 pycom17g02550 pycom17g02560 pycom17g02570 pycom17g02580 pycom17g02630 pycom17g02640 pycom17g04970 pycom17g04980 pycom17g05010 pycom17g07090 pycom17g07620 pycom17g07630 pycom17g07640 pycom17g07650 pycom17g07660 pycom17g07740 pycom17g07760 pycom17g07770 pycom17g09580 pycom17g11170 pycom17g11200 pycom17g11220 pycom17g11240 pycom17g11250 pycom17g11300 pycom17g11340 pycom17g11380 pycom17g11440
rosa_chinensis RchiOBHm_Chr1g0332901 RchiOBHm_Chr1g0338981 RchiOBHm_Chr1g0338991 RchiOBHm_Chr1g0341201 RchiOBHm_Chr1g0341211 RchiOBHm_Chr1g0342721 RchiOBHm_Chr1g0343191 RchiOBHm_Chr2g0146761 RchiOBHm_Chr2g0161791 RchiOBHm_Chr2g0161801 RchiOBHm_Chr2g0161841 RchiOBHm_Chr2g0162041 RchiOBHm_Chr2g0162651 RchiOBHm_Chr2g0162691 RchiOBHm_Chr2g0162701 RchiOBHm_Chr2g0162711 RchiOBHm_Chr2g0162721 RchiOBHm_Chr2g0162811 RchiOBHm_Chr2g0162831 RchiOBHm_Chr2g0162841 RchiOBHm_Chr4g0416381 RchiOBHm_Chr4g0416391 RchiOBHm_Chr5g0075981 RchiOBHm_Chr6g0276101 RchiOBHm_Chr7g0214581 RchiOBHm_Chr7g0214631 RchiOBHm_Chr7g0214651 RchiOBHm_Chr7g0214721 RchiOBHm_Chr7g0223161 RchiOBHm_Chr7g0230661 RchiOBHm_Chr7g0230751 RchiOBHm_Chr7g0230781 RchiOBHm_Chr7g0234581 RchiOBHm_Chr7g0234601 RchiOBHm_Chr7g0237561 RchiOBHm_Chr7g0237571 RchiOBHm_Chr7g0238781 RchiOBHm_Chr7g0238811 RchiOBHm_Chr7g0242161 RchiOBHm_Chr7g0242401 RchiOBHm_Chr7g0242451 RchiOBHm_Chr7g0242921 RchiOBHm_Chr7g0242981 RchiOBHm_Chr7g0243111 RchiOBHm_Chr7g0243251 RchiOBHm_Chr7g0243331
rosa_laevigata RLG00000000803 RLG00000000807 RLG00000000809 RLG00000000811 RLG00000000814 RLG00000000854 RLG00000000859 RLG00000000864 RLG00000000994 RLG00000000995 RLG00000001458 RLG00000001461 RLG00000001474 RLG00000001476 RLG00000002729 RLG00000002730 RLG00000003505 RLG00000008871 RLG00000008873 RLG00000018513 RLG00000021300 RLG00000021303 RLG00000021318 RLG00000021356 RLG00000021357 RLG00000029070 RLG00000029071 RLG00000029171 RLG00000029189 RLG00000029190 RLG00000029640 RLG00000034635
rosa_multiflora Rmu_co8149086.1_g000001 Rmu_co8460577.1_g000001 Rmu_sc0000429.1_g000062 Rmu_sc0000429.1_g000071 Rmu_sc0000429.1_g000081 Rmu_sc0000863.1_g000020 Rmu_sc0001488.1_g000011 Rmu_sc0001545.1_g000007 Rmu_sc0001849.1_g000068 Rmu_sc0001993.1_g000003 Rmu_sc0002222.1_g000002 Rmu_sc0002308.1_g000053 Rmu_sc0002308.1_g000069 Rmu_sc0002480.1_g000007 Rmu_sc0003179.1_g000041 Rmu_sc0004213.1_g000011 Rmu_sc0007101.1_g000012 Rmu_sc0008565.1_g000011 Rmu_sc0009298.1_g000003 Rmu_sc0010407.1_g000009 Rmu_sc0010407.1_g000013 Rmu_sc0026966.1_g000004 Rmu_ssc0000152.1_g000012 Rmu_ssc0000152.1_g000014 Rmu_ssc0000295.1_g000021 Rmu_ssc0000295.1_g000026
rosa_roxburghii Rroxscaffold_1G00029600 Rroxscaffold_2G00087790 Rroxscaffold_2G00087800 Rroxscaffold_2G00088360 Rroxscaffold_2G00088500 Rroxscaffold_2G00088530 Rroxscaffold_2G00088590 Rroxscaffold_3G00221570 Rroxscaffold_3G00222190 Rroxscaffold_3G00222240 Rroxscaffold_3G00222880 Rroxscaffold_3G00222900 Rroxscaffold_3G00223990 Rroxscaffold_3G00229750 Rroxscaffold_3G00229760 Rroxscaffold_3G00245140 Rroxscaffold_3G00245150 Rroxscaffold_3G00245200 Rroxscaffold_3G00253520 Rroxscaffold_3G00253530 Rroxscaffold_4G00294500 Rroxscaffold_4G00311800 Rroxscaffold_4G00311830 Rroxscaffold_4G00311850 Rroxscaffold_4G00313180 Rroxscaffold_4G00313210 Rroxscaffold_4G00316270 Rroxscaffold_4G00317910 Rroxscaffold_4G00317920 Rroxscaffold_5G00349290 Rroxscaffold_5G00349300 Rroxscaffold_5G00349310 Rroxscaffold_5G00351950 Rroxscaffold_5G00351960 Rroxscaffold_5G00353390 Rroxscaffold_7G00192540
rosa_rugosa Rorug01G0102500 Rorug01G0102600 Rorug01G0146400.1 Rorug01G0146500.1 Rorug01G0159400.1 Rorug01G0159500.1 Rorug02G0491900 Rorug02G0492000 Rorug02G0492100 Rorug02G0492200 Rorug02G0492300 Rorug02G0492400 Rorug02G0492500 Rorug02G0492700 Rorug02G0498500 Rorug02G0498600 Rorug02G0498800 Rorug04G0006900 Rorug04G0053700 Rorug06G0002000 Rorug06G0002100 Rorug07G0304700 Rorug07G0304800 Rorug07G0304800 Rorug07G0304800 Rorug07G0305300 Rorug07G0305400 Rorug07G0305500 Rorug07G0305500 Rorug07G0318300 Rorug07G0318600 Rorug07G0323600
rosa_samantha Rh1AG128900 Rh1AG162500 Rh1AG163000 Rh1AG164000 Rh1AG164700 Rh1AG173200 Rh1AG173300 Rh1AG173800 Rh1BG098100 Rh1BG129800 Rh1BG130600 Rh1BG141300 Rh1BG141500 Rh1BG141600 Rh1CG122700 Rh1CG151900 Rh1CG152400 Rh1CG153100 Rh1CG153500 Rh1CG161400 Rh1CG161600 Rh1DG134200 Rh1DG173500 Rh1DG173700 Rh2AG558100 Rh2AG558500 Rh2AG560200 Rh2AG564700 Rh2AG564800 Rh2BG465600 Rh2BG571700 Rh2BG572000 Rh2BG573500 Rh2CG439800 Rh2CG541800 Rh2CG542100 Rh2CG542200 Rh2CG543800 Rh2CG547300 Rh2DG474700 Rh2DG580600 Rh2DG580900 Rh2DG581200 Rh2DG581700 Rh2DG581800 Rh2DG583500 Rh2DG587200 Rh2DG587300 Rh2DG587400 Rh4AG128900 Rh4AG203800 Rh4AG319900 Rh4AG320000 Rh4BG122200 Rh4BG201100 Rh4CG136000 Rh4DG122400 Rh4DG201100 Rh4DG323600 Rh6BG077000 Rh6DG206200 Rh7AG282300 Rh7AG282500 Rh7AG283400 Rh7AG358600 Rh7AG414500 Rh7AG415200 Rh7AG415500 Rh7AG461700 Rh7AG469300 Rh7AG477800 Rh7BG273900 Rh7BG274000 Rh7BG390400 Rh7BG390500 Rh7BG391500 Rh7BG391800 Rh7BG408300 Rh7BG432000 Rh7BG439200 Rh7BG449200 Rh7BG449900 Rh7BG450400 Rh7BG450500 Rh7CG220900 Rh7CG301600 Rh7CG302700 Rh7CG377000 Rh7CG433900 Rh7CG434200 Rh7CG478800 Rh7DG286200 Rh7DG354000 Rh7DG424600 Rh7DG448000 Rh7DG462800 Rh7DG462900
rosa_wichuraiana Rw0G003440 Rw0G011970 Rw0G017120 Rw0G017130 Rw0G017140 Rw0G018470 Rw0G018480 Rw0G020440 Rw1G010470 Rw2G046180 Rw2G046210 Rw2G046230 Rw2G046390 Rw2G046400 Rw2G046710 Rw2G046720 Rw2G046730 Rw3G025310 Rw4G010410 Rw4G017250 Rw6G018270 Rw7G018180 Rw7G024130 Rw7G024160 Rw7G024170 Rw7G034060 Rw7G034250 Rw7G034260 Rw7G034400 Rw7G034410 Rw7G038210 Rw7G039020 Rw7G039060 Rw7G039280 Rw7G039400 Rw7G039470 Rw7G039880 Rw7G039890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1164
AccI GTMKAC 3 cut(s) 218, 765, 1171
AciI CCGC 3 cut(s) 204, 953, 1968
AclWI GGATC 5 cut(s) 148, 506, 519, 532, 1266
AcoI YGGCCR 1 cut(s) 1360
AcsI RAATTY 9 cut(s) 195, 291, 1082, 1513, 1634, 1687, 1716, 2088, 2128
AcuI CTGAAG 6 cut(s) 60, 123, 326, 393, 2052, 2300
AcvI CACGTG 1 cut(s) 1832
AfaI GTAC 4 cut(s) 92, 370, 1326, 1782
AfiI CCNNNNNNNGG 6 cut(s) 349, 521, 555, 781, 962, 1051
AhdI GACNNNNNGTC 1 cut(s) 336
AjiI CACGTC 2 cut(s) 790, 2097
AjnI CCWGG 6 cut(s) 136, 254, 548, 1050, 1963, 2341
AjuI GAANNNNNNNTTGG 2 cut(s) 386, 418
AleI CACNNNNGTG 1 cut(s) 466
AloI GAACNNNNNNTCC 2 cut(s) 260, 292
AluBI AGCT 8 cut(s) 506, 533, 638, 710, 1375, 1454, 1466, 1901
AluI AGCT 8 cut(s) 506, 533, 638, 710, 1375, 1454, 1466, 1901
Alw21I GWGCWC 2 cut(s) 693, 1924
Alw26I GTCTC 2 cut(s) 25, 1391
AlwI GGATC 5 cut(s) 148, 506, 519, 532, 1266
Ama87I CYCGRG 1 cut(s) 78
AoxI GGCC 5 cut(s) 206, 909, 1360, 1987, 2206
ApeKI GCWGC 9 cut(s) 545, 990, 993, 1451, 1672, 1682, 1898, 1946, 1949
ApoI RAATTY 9 cut(s) 195, 291, 1082, 1513, 1634, 1687, 1716, 2088, 2128
Asp700I GAANNNNTTC 2 cut(s) 275, 1810
AspS9I GGNCC 3 cut(s) 206, 910, 2339
AsuC2I CCSGG 1 cut(s) 913
AsuHPI GGTGA 5 cut(s) 332, 458, 1403, 1456, 2272
AsuII TTCGAA 1 cut(s) 1461
AvaI CYCGRG 1 cut(s) 78
AvaII GGWCC 1 cut(s) 2339
BalI TGGCCA 1 cut(s) 1362
BamHI GGATCC 1 cut(s) 524
BbrPI CACGTG 1 cut(s) 1832
BbsI GAAGAC 3 cut(s) 327, 792, 1297
Bbv12I GWGCWC 2 cut(s) 693, 1924
BbvCI CCTCAGC 1 cut(s) 1299
BbvI GCAGC 9 cut(s) 557, 977, 980, 1463, 1659, 1694, 1910, 1958, 1961
BccI CCATC 5 cut(s) 419, 793, 1621, 1858, 1943
BciT130I CCWGG 6 cut(s) 138, 256, 550, 1052, 1965, 2343
BcnI CCSGG 1 cut(s) 913
BcoDI GTCTC 2 cut(s) 25, 1391
BfaI CTAG 6 cut(s) 507, 885, 1787, 1803, 2003, 2268
BfmI CTRYAG 5 cut(s) 12, 1215, 1594, 1751, 1908
BglII AGATCT 1 cut(s) 1590
BisI GCNGC 9 cut(s) 546, 991, 994, 1452, 1673, 1683, 1899, 1947, 1950
BlsI GCNGC 9 cut(s) 547, 992, 995, 1453, 1674, 1684, 1900, 1948, 1951
BmcAI AGTACT 1 cut(s) 1326
Bme1390I CCNGG 7 cut(s) 138, 256, 550, 913, 1052, 1965, 2343
Bme18I GGWCC 1 cut(s) 2339
BmeRI GACNNNNNGTC 1 cut(s) 336
BmeT110I CYCGRG 1 cut(s) 78
BmgBI CACGTC 2 cut(s) 790, 2097
BmgT120I GGNCC 3 cut(s) 206, 910, 2339
BmiI GGNNCC 4 cut(s) 132, 526, 967, 1981
BmrFI CCNGG 7 cut(s) 138, 256, 550, 913, 1052, 1965, 2343
BmsI GCATC 7 cut(s) 484, 568, 849, 890, 1098, 1861, 2286
BpiI GAAGAC 3 cut(s) 327, 792, 1297
BpmI CTGGAG 2 cut(s) 918, 1680
Bpu10I CCTNAGC 1 cut(s) 1299
Bpu14I TTCGAA 1 cut(s) 1461
BpuMI CCSGG 1 cut(s) 913
BsaAI YACGTR 1 cut(s) 1832
BsaJI CCNNGG 5 cut(s) 174, 255, 970, 1357, 1963
BsaWI WCCGGW 1 cut(s) 2275
BsaXI ACNNNNNCTCC 6 cut(s) 118, 148, 260, 290, 392, 422
Bsc4I CCNNNNNNNGG 6 cut(s) 349, 521, 555, 781, 962, 1051
Bse1I ACTGG 3 cut(s) 869, 935, 1697
Bse3DI GCAATG 2 cut(s) 448, 1797
BseBI CCWGG 6 cut(s) 138, 256, 550, 1052, 1965, 2343
BseDI CCNNGG 5 cut(s) 174, 255, 970, 1357, 1963
BseGI GGATG 4 cut(s) 1113, 1636, 1876, 2301
BseLI CCNNNNNNNGG 6 cut(s) 349, 521, 555, 781, 962, 1051
BseMI GCAATG 2 cut(s) 448, 1797
BseMII CTCAG 1 cut(s) 1290
BseNI ACTGG 3 cut(s) 869, 935, 1697
BseRI GAGGAG 1 cut(s) 1564
BseXI GCAGC 9 cut(s) 557, 977, 980, 1463, 1659, 1694, 1910, 1958, 1961
BsgI GTGCAG 3 cut(s) 837, 1658, 1701
BshFI GGCC 5 cut(s) 208, 911, 1362, 1989, 2208
BsiHKAI GWGCWC 2 cut(s) 693, 1924
BsiHKCI CYCGRG 1 cut(s) 78
BsiSI CCGG 3 cut(s) 913, 956, 2276
BslFI GGGAC 1 cut(s) 20
BslI CCNNNNNNNGG 6 cut(s) 349, 521, 555, 781, 962, 1051
BsmAI GTCTC 2 cut(s) 25, 1391
BsmFI GGGAC 1 cut(s) 20
BsmI GAATGC 5 cut(s) 175, 463, 1111, 1952, 2256
BsnI GGCC 5 cut(s) 208, 911, 1362, 1989, 2208
BsoBI CYCGRG 1 cut(s) 78
Bsp119I TTCGAA 1 cut(s) 1461
Bsp1286I GDGCHC 2 cut(s) 693, 1924
Bsp143I GATC 5 cut(s) 140, 511, 524, 1258, 1590
Bsp19I CCATGG 1 cut(s) 1357
BspACI CCGC 3 cut(s) 204, 953, 1968
BspANI GGCC 5 cut(s) 208, 911, 1362, 1989, 2208
BspCNI CTCAG 1 cut(s) 1291
BspHI TCATGA 1 cut(s) 1204
BspLI GGNNCC 4 cut(s) 132, 526, 967, 1981
BspPI GGATC 5 cut(s) 148, 506, 519, 532, 1266
BspQI GCTCTTC 2 cut(s) 540, 645
BspT104I TTCGAA 1 cut(s) 1461
BsrDI GCAATG 2 cut(s) 448, 1797
BsrI ACTGG 3 cut(s) 869, 935, 1697
BssECI CCNNGG 5 cut(s) 174, 255, 970, 1357, 1963
BssMI GATC 5 cut(s) 140, 511, 524, 1258, 1590
BssNAI GTATAC 2 cut(s) 219, 1172
BssT1I CCWWGG 3 cut(s) 174, 970, 1357
Bst1107I GTATAC 2 cut(s) 219, 1172
Bst2UI CCWGG 6 cut(s) 138, 256, 550, 1052, 1965, 2343
Bst6I CTCTTC 6 cut(s) 540, 645, 1072, 1389, 1581, 2001
BstBAI YACGTR 1 cut(s) 1832
BstBI TTCGAA 1 cut(s) 1461
BstC8I GCNNGC 1 cut(s) 206
BstDEI CTNAG 1 cut(s) 1299
BstDSI CCRYGG 1 cut(s) 1357
BstEII GGTNACC 1 cut(s) 2278
BstF5I GGATG 4 cut(s) 1113, 1636, 1876, 2301
BstKTI GATC 5 cut(s) 143, 514, 527, 1261, 1593
BstMAI GTCTC 2 cut(s) 25, 1391
BstMBI GATC 5 cut(s) 140, 511, 524, 1258, 1590
BstMWI GCNNNNNNNGC 3 cut(s) 251, 503, 851
BstNI CCWGG 6 cut(s) 138, 256, 550, 1052, 1965, 2343
BstNSI RCATGY 1 cut(s) 668
BstPI GGTNACC 1 cut(s) 2278
BstSCI CCNGG 7 cut(s) 136, 254, 548, 911, 1050, 1963, 2341
BstSFI CTRYAG 5 cut(s) 12, 1215, 1594, 1751, 1908
BstV1I GCAGC 9 cut(s) 557, 977, 980, 1463, 1659, 1694, 1910, 1958, 1961
BstV2I GAAGAC 3 cut(s) 327, 792, 1297
BstX2I RGATCY 4 cut(s) 511, 524, 1258, 1590
BstYI RGATCY 4 cut(s) 511, 524, 1258, 1590
BstZ17I GTATAC 2 cut(s) 219, 1172
BsuRI GGCC 5 cut(s) 208, 911, 1362, 1989, 2208
BtgI CCRYGG 1 cut(s) 1357
BtgZI GCGATG 1 cut(s) 826
BtrI CACGTC 2 cut(s) 790, 2097
BtsCI GGATG 4 cut(s) 1113, 1636, 1876, 2301
BtsIMutI CAGTG 3 cut(s) 1684, 1760, 2375
Cac8I GCNNGC 1 cut(s) 206
CciI TCATGA 1 cut(s) 1204
Cfr13I GGNCC 3 cut(s) 206, 910, 2339
Csp6I GTAC 4 cut(s) 91, 369, 1325, 1781
CviQI GTAC 4 cut(s) 91, 369, 1325, 1781
DdeI CTNAG 1 cut(s) 1299
DpnI GATC 5 cut(s) 142, 513, 526, 1260, 1592
DpnII GATC 5 cut(s) 140, 511, 524, 1258, 1590
DriI GACNNNNNGTC 1 cut(s) 336
EaeI YGGCCR 1 cut(s) 1360
Eam1104I CTCTTC 6 cut(s) 540, 645, 1072, 1389, 1581, 2001
Eam1105I GACNNNNNGTC 1 cut(s) 336
EarI CTCTTC 6 cut(s) 540, 645, 1072, 1389, 1581, 2001
EciI GGCGGA 2 cut(s) 942, 1983
Eco130I CCWWGG 3 cut(s) 174, 970, 1357
Eco147I AGGCCT 1 cut(s) 1989
Eco47I GGWCC 1 cut(s) 2339
Eco57I CTGAAG 6 cut(s) 60, 123, 326, 393, 2052, 2300
Eco72I CACGTG 1 cut(s) 1832
Eco88I CYCGRG 1 cut(s) 78
Eco91I GGTNACC 1 cut(s) 2278
EcoO65I GGTNACC 1 cut(s) 2278
EcoRI GAATTC 1 cut(s) 2088
EcoRII CCWGG 6 cut(s) 136, 254, 548, 1050, 1963, 2341
EcoT14I CCWWGG 3 cut(s) 174, 970, 1357
EcoT22I ATGCAT 2 cut(s) 1113, 1797
ErhI CCWWGG 3 cut(s) 174, 970, 1357
FalI AAGNNNNNCTT 2 cut(s) 77, 109
FaqI GGGAC 1 cut(s) 20
FauI CCCGC 1 cut(s) 197
FblI GTMKAC 3 cut(s) 218, 765, 1171
Fnu4HI GCNGC 9 cut(s) 546, 991, 994, 1452, 1673, 1683, 1899, 1947, 1950
FokI GGATG 4 cut(s) 1120, 1643, 1883, 2308
Fsp4HI GCNGC 9 cut(s) 546, 991, 994, 1452, 1673, 1683, 1899, 1947, 1950
FspBI CTAG 6 cut(s) 507, 885, 1787, 1803, 2003, 2268
GluI GCNGC 9 cut(s) 546, 991, 994, 1452, 1673, 1683, 1899, 1947, 1950
GsuI CTGGAG 2 cut(s) 918, 1680
HaeIII GGCC 5 cut(s) 208, 911, 1362, 1989, 2208
HapII CCGG 3 cut(s) 913, 956, 2276
HindIII AAGCTT 1 cut(s) 708
HinfI GANTC 8 cut(s) 233, 407, 484, 700, 1274, 1521, 1799, 1849
HpaII CCGG 3 cut(s) 913, 956, 2276
HphI GGTGA 5 cut(s) 332, 458, 1403, 1456, 2272
Hpy166II GTNNAC 7 cut(s) 219, 302, 369, 766, 1172, 1447, 1835
Hpy188I TCNGA 8 cut(s) 145, 1107, 1234, 1289, 1322, 1816, 2038, 2319
Hpy8I GTNNAC 7 cut(s) 219, 302, 369, 766, 1172, 1447, 1835
HpyAV CCTTC 5 cut(s) 368, 857, 1238, 2000, 2321
HpyCH4IV ACGT 5 cut(s) 89, 298, 789, 1831, 2096
HpyF10VI GCNNNNNNNGC 3 cut(s) 251, 503, 851
HpyF3I CTNAG 1 cut(s) 1299
HpySE526I ACGT 5 cut(s) 89, 298, 789, 1831, 2096
Kzo9I GATC 5 cut(s) 140, 511, 524, 1258, 1590
LguI GCTCTTC 2 cut(s) 540, 645
Lsp1109I GCAGC 9 cut(s) 557, 977, 980, 1463, 1659, 1694, 1910, 1958, 1961
LweI GCATC 7 cut(s) 484, 568, 849, 890, 1098, 1861, 2286
MaeI CTAG 6 cut(s) 507, 885, 1787, 1803, 2003, 2268
MaeII ACGT 5 cut(s) 89, 298, 789, 1831, 2096
MaeIII GTNAC 7 cut(s) 338, 629, 928, 1492, 1930, 2097, 2278
MalI GATC 5 cut(s) 142, 513, 526, 1260, 1592
MboI GATC 5 cut(s) 140, 511, 524, 1258, 1590
MfeI CAATTG 1 cut(s) 30
MflI RGATCY 4 cut(s) 511, 524, 1258, 1590
MhlI GDGCHC 2 cut(s) 693, 1924
MlsI TGGCCA 1 cut(s) 1362
MluNI TGGCCA 1 cut(s) 1362
MlyI GAGTC 3 cut(s) 401, 709, 1515
Mox20I TGGCCA 1 cut(s) 1362
Mph1103I ATGCAT 2 cut(s) 1113, 1797
MroXI GAANNNNTTC 2 cut(s) 275, 1810
MscI TGGCCA 1 cut(s) 1362
MseI TTAA 6 cut(s) 84, 192, 999, 1062, 1124, 1197
MslI CAYNNNNRTG 3 cut(s) 466, 2141, 2186
Msp20I TGGCCA 1 cut(s) 1362
MspI CCGG 3 cut(s) 913, 956, 2276
MspR9I CCNGG 7 cut(s) 138, 256, 550, 913, 1052, 1965, 2343
MunI CAATTG 1 cut(s) 30
Mva1269I GAATGC 5 cut(s) 175, 463, 1111, 1952, 2256
MvaI CCWGG 6 cut(s) 138, 256, 550, 1052, 1965, 2343
MwoI GCNNNNNNNGC 3 cut(s) 251, 503, 851
NciI CCSGG 1 cut(s) 913
NcoI CCATGG 1 cut(s) 1357
NdeII GATC 5 cut(s) 140, 511, 524, 1258, 1590
NlaIV GGNNCC 4 cut(s) 132, 526, 967, 1981
NmuCI GTSAC 4 cut(s) 338, 928, 2097, 2278
NsiI ATGCAT 2 cut(s) 1113, 1797
NspI RCATGY 1 cut(s) 668
NspV TTCGAA 1 cut(s) 1461
OliI CACNNNNGTG 1 cut(s) 466
PaeR7I CTCGAG 1 cut(s) 78
PagI TCATGA 1 cut(s) 1204
PceI AGGCCT 1 cut(s) 1989
PciSI GCTCTTC 2 cut(s) 540, 645
PctI GAATGC 5 cut(s) 175, 463, 1111, 1952, 2256
PdmI GAANNNNTTC 2 cut(s) 275, 1810
PfeI GAWTC 5 cut(s) 233, 484, 1274, 1799, 1849
PfoI TCCNGGA 1 cut(s) 136
PkrI GCNGC 9 cut(s) 547, 992, 995, 1453, 1674, 1684, 1900, 1948, 1951
PleI GAGTC 3 cut(s) 401, 708, 1515
PmaCI CACGTG 1 cut(s) 1832
PmlI CACGTG 1 cut(s) 1832
PpsI GAGTC 3 cut(s) 401, 708, 1515
Ppu21I YACGTR 1 cut(s) 1832
PsiI TTATAA 1 cut(s) 1164
Psp6I CCWGG 6 cut(s) 136, 254, 548, 1050, 1963, 2341
PspCI CACGTG 1 cut(s) 1832
PspEI GGTNACC 1 cut(s) 2278
PspGI CCWGG 6 cut(s) 136, 254, 548, 1050, 1963, 2341
PspN4I GGNNCC 4 cut(s) 132, 526, 967, 1981
PspPI GGNCC 3 cut(s) 206, 910, 2339
PsuI RGATCY 4 cut(s) 511, 524, 1258, 1590
RsaI GTAC 4 cut(s) 92, 370, 1326, 1782
RsaNI GTAC 4 cut(s) 91, 369, 1325, 1781
RseI CAYNNNNRTG 3 cut(s) 466, 2141, 2186
SapI GCTCTTC 2 cut(s) 540, 645
SaqAI TTAA 6 cut(s) 84, 192, 999, 1062, 1124, 1197
SatI GCNGC 9 cut(s) 546, 991, 994, 1452, 1673, 1683, 1899, 1947, 1950
Sau3AI GATC 5 cut(s) 140, 511, 524, 1258, 1590
Sau96I GGNCC 3 cut(s) 206, 910, 2339
ScaI AGTACT 1 cut(s) 1326
SchI GAGTC 3 cut(s) 401, 709, 1515
ScrFI CCNGG 7 cut(s) 138, 256, 550, 913, 1052, 1965, 2343
SduI GDGCHC 2 cut(s) 693, 1924
SfaNI GCATC 7 cut(s) 484, 568, 849, 890, 1098, 1861, 2286
SfcI CTRYAG 5 cut(s) 12, 1215, 1594, 1751, 1908
Sfr274I CTCGAG 1 cut(s) 78
SfuI TTCGAA 1 cut(s) 1461
SinI GGWCC 1 cut(s) 2339
SlaI CTCGAG 1 cut(s) 78
SmiMI CAYNNNNRTG 3 cut(s) 466, 2141, 2186
SmlI CTYRAG 1 cut(s) 78
SmoI CTYRAG 1 cut(s) 78
SseBI AGGCCT 1 cut(s) 1989
SsiI CCGC 3 cut(s) 204, 953, 1968
SspI AATATT 2 cut(s) 2234, 2349
SspMI CTAG 6 cut(s) 507, 885, 1787, 1803, 2003, 2268
StuI AGGCCT 1 cut(s) 1989
StyD4I CCNGG 7 cut(s) 136, 254, 548, 911, 1050, 1963, 2341
StyI CCWWGG 3 cut(s) 174, 970, 1357
TaiI ACGT 5 cut(s) 92, 301, 792, 1834, 2099
TaqI TCGA 7 cut(s) 79, 123, 405, 894, 1371, 1461, 1527
TatI WGTACW 2 cut(s) 368, 1324
TfiI GAWTC 5 cut(s) 233, 484, 1274, 1799, 1849
Tru1I TTAA 6 cut(s) 84, 192, 999, 1062, 1124, 1197
Tru9I TTAA 6 cut(s) 84, 192, 999, 1062, 1124, 1197
TscAI CASTG 3 cut(s) 1684, 1760, 2375
TseFI GTSAC 4 cut(s) 338, 928, 2097, 2278
TseI GCWGC 9 cut(s) 545, 990, 993, 1451, 1672, 1682, 1898, 1946, 1949
Tsp45I GTSAC 4 cut(s) 338, 928, 2097, 2278
TspGWI ACGGA 3 cut(s) 143, 537, 916
TspRI CASTG 3 cut(s) 1684, 1760, 2375
VpaK11BI GGWCC 1 cut(s) 2339
XapI RAATTY 9 cut(s) 195, 291, 1082, 1513, 1634, 1687, 1716, 2088, 2128
XbaI TCTAGA 1 cut(s) 1802
XceI RCATGY 1 cut(s) 668
XhoI CTCGAG 1 cut(s) 78
XmiI GTMKAC 3 cut(s) 218, 765, 1171
XmnI GAANNNNTTC 2 cut(s) 275, 1810
XspI CTAG 6 cut(s) 507, 885, 1787, 1803, 2003, 2268
ZrmI AGTACT 1 cut(s) 1326
Zsp2I ATGCAT 2 cut(s) 1113, 1797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.