Rroxscaffold_3G00223990

protein ubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000027
Physical Location & Seq
Reverse (-)
7154765 .. 7174719
19955 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00223990.1

Sequence Viewer

Length: 2274 bp
ATGGCGAGCATTACAGTTGCCTCCAATTCAATTGTCTTGGAAGTCCTTAACCAACATAACTATAAAGAATGGAGTTCTCGGGTTGAAACTTACTTGTTGGCCGAAGATCTTTGGGATGTCGTCAACATGAAACCTCCTGAACGAGAAGAGAGAGAAGTTGAATACAAGGCTTGGAAGACAAAGAATGCCAAGGCTTTACATGCAATTAAAATTTCTTGCGGGACTCAACCATTTTCTTTTATCAATGATACCCGCACAGCCAAGATGGCCTGGGAGGTCTTGGAGAAACAGTTCAAATCACGTGACACATTACGTTTAGAAAAAAAAAAGAGAGAAGAGAGAAACAGATATCTTTATACCAAAATCGATGATGCGAGCGAGGAGTTGACAACAGCTGATGAAGAGCCAGGAAATCAGAACCGTTCCAACTATCACTTCGACTCCAGCTATGAACCTTTCTTTGATTCTGTTGTGAATGGTGACTGGGACAATGCTAAGGAGTGCCTTGCTACATTACTACTTCCTAATGTACTAAGAGCAAGAAGTCCAGCAGCTAGAGGATCTACAGCTCTTCACATTGCAGCGAGGGAAGGGCATGTGCATATTGTAAAAGAGTTGGTGTTGTTGATGACTCAAGAAGATCTGGAAATAAGAGATGATAATGGTTGCACCGCTCTTCACATAGCAGTACAGAAGGAGAACCTGGACGCTGTGAAAGAGGTGGTGCCGTTGATGAGACAACAGGGCTTGGGAATAAAAAACAAAAATTATGGTGAAACTGCTCTTCATGTAGCAGCTGGGAGTGGGAATGTGCGTATTGCGAAAGAGTTGGGAGAGTTGATGAGGGAAGAAGATTTGGAAATAGGAAACTCCAACAATTGCCACGCTCTTCACATAGCACTCTTGAAAGGGCATGTGCATATTGGGAAAGTGTTGGTAGCATTGATGAGAAAAGAAGCTTTGGAAATAAATGATGGACAAGGCTATACGGCTTTAGGTCGTGCCATAAGGTTATGCCACCAGCGAGGAGACGAGTACAGTGAAGGAGCTGTGATCGAAATGATTAAACCCATGGTTGCAAAGAACCAGAAAATACTCGGCATTGTGGCTTCTCCCAATGACTACATTCCAGTTGTGGAGGCTTGCATGTTGTTAAAATTCCAATTGGGCCGCTATCTCTATTCCGTTACTCCACGGGAAGCTTTAATGCCGCCCAAGAACAGATGGCAAGGTGCTTCACTATTTGTGTTTTGTCTTGGAGCGAAGGAATTTGATATTCCGTGGGACTTACTTTCAAATTGCCCAAGGTTGGCCACGGTTGAAACTGTACATGGAGGCAATTCTCCGATCTTGGTATTGTCTCGTATGCACTCTGCATTCTTAAGTGGAATGCAACTCAAATTTTGGCAAAAATGGATCTATGAAAGGATAAATATAAAAGCTCCCGCTATTTTTGATATTCATGATCTTTCTATACCTGTTTCAATCTCAGAAGATGATGGAGGTACTCAAAAGGATGTCGTTGTCTCAGCTGTGAGTTTACATAAAAGGCTCGTTAAAAGCCTCCTTGATGTTTTGGGGAATTTATCTCCCCTTTCACAATCTAATCATATTCAAGGTGCAAGTTTGCAAATGCAAAGAGAATTGCAATGGTTTAAGGAGGTCGAGAGTATTGTTCCTCTAGCAGCACACGAGACTCGAAATACTTCAGATGATTTGACTGCTCGTGAACTATTTCATAAGAATCACGAGAAATTGATGGTGGAGGCAGAAATATCAATGAAAGGGACAGCAACTTCTTGTACAGTTGTAGGTGCTTTGATTGTTACCATCATGTTTGCAGCAGCATTCACAATTCCTGGTGGAAATAATGGTACATCAGGCCTTCCAATGTTCATAAACGAAAACTTGTTTTTGGTGTTTATAGCTTCAGATACTATATCCCTCATTTTTTCCACAACTTCAGTTATTATATTTCTGGGAATCCTCACATCACGTTATGCAGAAGAGGATTTCCTCCAAAACTTACCCACAAAGATGATGATAGGTCTTTTAACCCTTTTCATTGCTATTGCCACCATGATGATCGCATTTTCTTGTGGCCTTGTCATTATGCTTCATGGAAAATATTCATGGGTTCCTATTCCAGGCATCTTGGTTGCTAGTATCCCAGTGACCTCATTTGTGTGGATGTTGTTTCCCCTCCTTGTGCAGACTTTCATTTCTACTTATGGACCGGGAATATTCAACAGGAAAGTCAAACAGTGGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

757

Amino Acids

84.79

Weight (kDa)

6.36

Isoelectric Point (pI)

38.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4219 PF13961 16 - 42 3.8e-08 Domain of unknown function (DUF4219)
Retrotran_gag_2 PF14223 29 - 105 7.4e-10 gag-polypeptide of LTR copia-type
Ank_2 PF12796 169 - 241 1.3e-11 Ankyrin repeats (3 copies)
Ank_5 PF13857 186 - 229 1e-05 Ankyrin repeats (many copies)
Ank_4 PF13637 191 - 239 5.2e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 209 - 277 4.1e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 248 - 313 4e-07 Ankyrin repeats (3 copies)
PGG PF13962 594 - 704 1.9e-22 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000027)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18670 AT3G18670 AT3G54065
fragaria_vesca FvH4_1g26570 FvH4_1g26570 FvH4_5g19270 FvH4_5g19273 FvH4_5g35840 FvH4_5g36340 FvH4_5g36630 FvH4_5g37181 FvH4_5g37182 FvH4_5g37185 FvH4_5g37240 FvH4_5g37260 FvH4_6g21440 FvH4_6g44571 FvH4_6g44572 FvH4_6g44610 FvH4_6g44620 FvH4_6g44650 FvH4_6g44660 FvH4_6g45151 FvH4_6g45152 FvH4_6g45152 FvH4_6g45153 FvH4_6g45153 FvH4_6g45153 FvH4_6g45153 FvH4_6g45153 FvH4_6g45154 FvH4_6g51440 FvH4_7g08090 FvH4_7g08090 FvH4_7g08090 FvH4_7g08090 FvH4_7g08090 FvH4_7g08090
malus_domestica MD00G1010300.v1.1 MD00G1010400.v1.1 MD00G1010500.v1.1 MD00G1011000.v1.1 MD00G1011200.v1.1 MD00G1011500.v1.1 MD00G1011900.v1.1 MD03G1133900.v1.1 MD03G1134100.v1.1 MD09G1086300.v1.1 MD09G1086500.v1.1 MD09G1086900.v1.1 MD09G1087000.v1.1 MD09G1088000.v1.1 MD09G1088100.v1.1 MD09G1088200.v1.1 MD09G1088300.v1.1 MD09G1089000.v1.1 MD09G1089300.v1.1 MD09G1133800.v1.1 MD16G1219400.v1.1 MD17G1027900.v1.1 MD17G1028400.v1.1 MD17G1028600.v1.1 MD17G1028700.v1.1 MD17G1028900.v1.1 MD17G1029000.v1.1 MD17G1029100.v1.1 MD17G1029200.v1.1 MD17G1075400.v1.1 MD17G1076000.v1.1 MD17G1077000.v1.1 MD17G1077100.v1.1 MD17G1077800.v1.1 MD17G1078000.v1.1 MD17G1078100.v1.1 MD17G1078300.v1.1 MD17G1100900.v1.1 MD17G1101100.v1.1 MD17G1101400.v1.1 MD17G1104200.v1.1 MD17G1104500.v1.1 MD17G1121300.v1.1 MD17G1121600.v1.1 MD17G1121900.v1.1 MD17G1122700.v1.1 MD17G1218800.v1.1
prunus_persica Prupe.2G033800_v2.0.a1 Prupe.2G033900_v2.0.a1 Prupe.2G080300_v2.0.a1 Prupe.2G080700_v2.0.a1 Prupe.2G080900_v2.0.a1 Prupe.2G081100_v2.0.a1 Prupe.2G081100_v2.0.a1 Prupe.2G081200_v2.0.a1 Prupe.2G081200_v2.0.a1 Prupe.2G081200_v2.0.a1 Prupe.2G081200_v2.0.a1 Prupe.2G081700_v2.0.a1 Prupe.2G081700_v2.0.a1 Prupe.2G082000_v2.0.a1 Prupe.2G082100_v2.0.a1 Prupe.2G082100_v2.0.a1 Prupe.2G082200_v2.0.a1 Prupe.2G082300_v2.0.a1 Prupe.2G082700_v2.0.a1 Prupe.2G082800_v2.0.a1 Prupe.2G082900_v2.0.a1 Prupe.3G235600_v2.0.a1 Prupe.3G236300_v2.0.a1 Prupe.3G236300_v2.0.a1 Prupe.3G236600_v2.0.a1 Prupe.3G236600_v2.0.a1 Prupe.3G236800_v2.0.a1 Prupe.3G237000_v2.0.a1 Prupe.3G237100_v2.0.a1 Prupe.3G237300_v2.0.a1 Prupe.3G237400_v2.0.a1 Prupe.3G237500_v2.0.a1 Prupe.3G237500_v2.0.a1 Prupe.3G237500_v2.0.a1 Prupe.4G282500_v2.0.a1 Prupe.4G282500_v2.0.a1
pyrus_communis pycom03g08990 pycom03g09000 pycom09g01180 pycom09g01190 pycom09g01220 pycom09g01230 pycom09g01360 pycom09g01370 pycom09g01380 pycom09g01410 pycom09g05500 pycom11g10280 pycom11g10290 pycom11g10310 pycom16g18270 pycom16g18280 pycom17g02280 pycom17g02290 pycom17g02300 pycom17g02360 pycom17g02390 pycom17g02410 pycom17g02440 pycom17g02460 pycom17g02470 pycom17g02480 pycom17g02510 pycom17g02530 pycom17g02540 pycom17g02550 pycom17g02560 pycom17g02570 pycom17g02580 pycom17g02630 pycom17g02640 pycom17g04970 pycom17g04980 pycom17g05010 pycom17g07090 pycom17g07620 pycom17g07630 pycom17g07640 pycom17g07650 pycom17g07660 pycom17g07740 pycom17g07760 pycom17g07770 pycom17g09580 pycom17g11170 pycom17g11200 pycom17g11220 pycom17g11240 pycom17g11250 pycom17g11300 pycom17g11340 pycom17g11380 pycom17g11440
rosa_chinensis RchiOBHm_Chr1g0332901 RchiOBHm_Chr1g0338981 RchiOBHm_Chr1g0338991 RchiOBHm_Chr1g0341201 RchiOBHm_Chr1g0341211 RchiOBHm_Chr1g0342721 RchiOBHm_Chr1g0343191 RchiOBHm_Chr2g0146761 RchiOBHm_Chr2g0161791 RchiOBHm_Chr2g0161801 RchiOBHm_Chr2g0161841 RchiOBHm_Chr2g0162041 RchiOBHm_Chr2g0162651 RchiOBHm_Chr2g0162691 RchiOBHm_Chr2g0162701 RchiOBHm_Chr2g0162711 RchiOBHm_Chr2g0162721 RchiOBHm_Chr2g0162811 RchiOBHm_Chr2g0162831 RchiOBHm_Chr2g0162841 RchiOBHm_Chr4g0416381 RchiOBHm_Chr4g0416391 RchiOBHm_Chr5g0075981 RchiOBHm_Chr6g0276101 RchiOBHm_Chr7g0214581 RchiOBHm_Chr7g0214631 RchiOBHm_Chr7g0214651 RchiOBHm_Chr7g0214721 RchiOBHm_Chr7g0223161 RchiOBHm_Chr7g0230661 RchiOBHm_Chr7g0230751 RchiOBHm_Chr7g0230781 RchiOBHm_Chr7g0234581 RchiOBHm_Chr7g0234601 RchiOBHm_Chr7g0237561 RchiOBHm_Chr7g0237571 RchiOBHm_Chr7g0238781 RchiOBHm_Chr7g0238811 RchiOBHm_Chr7g0242161 RchiOBHm_Chr7g0242401 RchiOBHm_Chr7g0242451 RchiOBHm_Chr7g0242921 RchiOBHm_Chr7g0242981 RchiOBHm_Chr7g0243111 RchiOBHm_Chr7g0243251 RchiOBHm_Chr7g0243331
rosa_laevigata RLG00000000803 RLG00000000807 RLG00000000809 RLG00000000811 RLG00000000814 RLG00000000854 RLG00000000859 RLG00000000864 RLG00000000994 RLG00000000995 RLG00000001458 RLG00000001461 RLG00000001474 RLG00000001476 RLG00000002729 RLG00000002730 RLG00000003505 RLG00000008871 RLG00000008873 RLG00000018513 RLG00000021300 RLG00000021303 RLG00000021318 RLG00000021356 RLG00000021357 RLG00000029070 RLG00000029071 RLG00000029171 RLG00000029189 RLG00000029190 RLG00000029640 RLG00000034635
rosa_multiflora Rmu_co8149086.1_g000001 Rmu_co8460577.1_g000001 Rmu_sc0000429.1_g000062 Rmu_sc0000429.1_g000071 Rmu_sc0000429.1_g000081 Rmu_sc0000863.1_g000020 Rmu_sc0001488.1_g000011 Rmu_sc0001545.1_g000007 Rmu_sc0001849.1_g000068 Rmu_sc0001993.1_g000003 Rmu_sc0002222.1_g000002 Rmu_sc0002308.1_g000053 Rmu_sc0002308.1_g000069 Rmu_sc0002480.1_g000007 Rmu_sc0003179.1_g000041 Rmu_sc0004213.1_g000011 Rmu_sc0007101.1_g000012 Rmu_sc0008565.1_g000011 Rmu_sc0009298.1_g000003 Rmu_sc0010407.1_g000009 Rmu_sc0010407.1_g000013 Rmu_sc0026966.1_g000004 Rmu_ssc0000152.1_g000012 Rmu_ssc0000152.1_g000014 Rmu_ssc0000295.1_g000021 Rmu_ssc0000295.1_g000026
rosa_roxburghii Rroxscaffold_1G00029600 Rroxscaffold_2G00087790 Rroxscaffold_2G00087800 Rroxscaffold_2G00088360 Rroxscaffold_2G00088500 Rroxscaffold_2G00088530 Rroxscaffold_2G00088590 Rroxscaffold_3G00221570 Rroxscaffold_3G00222190 Rroxscaffold_3G00222240 Rroxscaffold_3G00222880 Rroxscaffold_3G00222900 Rroxscaffold_3G00223990 Rroxscaffold_3G00229750 Rroxscaffold_3G00229760 Rroxscaffold_3G00245140 Rroxscaffold_3G00245150 Rroxscaffold_3G00245200 Rroxscaffold_3G00253520 Rroxscaffold_3G00253530 Rroxscaffold_4G00294500 Rroxscaffold_4G00311800 Rroxscaffold_4G00311830 Rroxscaffold_4G00311850 Rroxscaffold_4G00313180 Rroxscaffold_4G00313210 Rroxscaffold_4G00316270 Rroxscaffold_4G00317910 Rroxscaffold_4G00317920 Rroxscaffold_5G00349290 Rroxscaffold_5G00349300 Rroxscaffold_5G00349310 Rroxscaffold_5G00351950 Rroxscaffold_5G00351960 Rroxscaffold_5G00353390 Rroxscaffold_7G00192540
rosa_rugosa Rorug01G0102500 Rorug01G0102600 Rorug01G0146400.1 Rorug01G0146500.1 Rorug01G0159400.1 Rorug01G0159500.1 Rorug02G0491900 Rorug02G0492000 Rorug02G0492100 Rorug02G0492200 Rorug02G0492300 Rorug02G0492400 Rorug02G0492500 Rorug02G0492700 Rorug02G0498500 Rorug02G0498600 Rorug02G0498800 Rorug04G0006900 Rorug04G0053700 Rorug06G0002000 Rorug06G0002100 Rorug07G0304700 Rorug07G0304800 Rorug07G0304800 Rorug07G0304800 Rorug07G0305300 Rorug07G0305400 Rorug07G0305500 Rorug07G0305500 Rorug07G0318300 Rorug07G0318600 Rorug07G0323600
rosa_samantha Rh1AG128900 Rh1AG162500 Rh1AG163000 Rh1AG164000 Rh1AG164700 Rh1AG173200 Rh1AG173300 Rh1AG173800 Rh1BG098100 Rh1BG129800 Rh1BG130600 Rh1BG141300 Rh1BG141500 Rh1BG141600 Rh1CG122700 Rh1CG151900 Rh1CG152400 Rh1CG153100 Rh1CG153500 Rh1CG161400 Rh1CG161600 Rh1DG134200 Rh1DG173500 Rh1DG173700 Rh2AG558100 Rh2AG558500 Rh2AG560200 Rh2AG564700 Rh2AG564800 Rh2BG465600 Rh2BG571700 Rh2BG572000 Rh2BG573500 Rh2CG439800 Rh2CG541800 Rh2CG542100 Rh2CG542200 Rh2CG543800 Rh2CG547300 Rh2DG474700 Rh2DG580600 Rh2DG580900 Rh2DG581200 Rh2DG581700 Rh2DG581800 Rh2DG583500 Rh2DG587200 Rh2DG587300 Rh2DG587400 Rh4AG128900 Rh4AG203800 Rh4AG319900 Rh4AG320000 Rh4BG122200 Rh4BG201100 Rh4CG136000 Rh4DG122400 Rh4DG201100 Rh4DG323600 Rh6BG077000 Rh6DG206200 Rh7AG282300 Rh7AG282500 Rh7AG283400 Rh7AG358600 Rh7AG414500 Rh7AG415200 Rh7AG415500 Rh7AG461700 Rh7AG469300 Rh7AG477800 Rh7BG273900 Rh7BG274000 Rh7BG390400 Rh7BG390500 Rh7BG391500 Rh7BG391800 Rh7BG408300 Rh7BG432000 Rh7BG439200 Rh7BG449200 Rh7BG449900 Rh7BG450400 Rh7BG450500 Rh7CG220900 Rh7CG301600 Rh7CG302700 Rh7CG377000 Rh7CG433900 Rh7CG434200 Rh7CG478800 Rh7DG286200 Rh7DG354000 Rh7DG424600 Rh7DG448000 Rh7DG462800 Rh7DG462900
rosa_wichuraiana Rw0G003440 Rw0G011970 Rw0G017120 Rw0G017130 Rw0G017140 Rw0G018470 Rw0G018480 Rw0G020440 Rw1G010470 Rw2G046180 Rw2G046210 Rw2G046230 Rw2G046390 Rw2G046400 Rw2G046710 Rw2G046720 Rw2G046730 Rw3G025310 Rw4G010410 Rw4G017250 Rw6G018270 Rw7G018180 Rw7G024130 Rw7G024160 Rw7G024170 Rw7G034060 Rw7G034250 Rw7G034260 Rw7G034400 Rw7G034410 Rw7G038210 Rw7G039020 Rw7G039060 Rw7G039280 Rw7G039400 Rw7G039470 Rw7G039880 Rw7G039890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 724
AccBSI CCGCTC 1 cut(s) 674
AciI CCGC 6 cut(s) 219, 253, 672, 1171, 1211, 1446
AclWI GGATC 2 cut(s) 568, 1424
AcoI YGGCCR 2 cut(s) 99, 1311
AcsI RAATTY 5 cut(s) 210, 1157, 1268, 1400, 1582
AcuI CTGAAG 3 cut(s) 1692, 1914, 1947
AcvI CACGTG 1 cut(s) 302
AfaI GTAC 7 cut(s) 531, 690, 1037, 1329, 1507, 1804, 1876
AfiI CCNNNNNNNGG 2 cut(s) 1309, 2147
AflII CTTAAG 1 cut(s) 1381
AjnI CCWGG 5 cut(s) 269, 406, 702, 1858, 2146
AjuI GAANNNNNNNTTGG 2 cut(s) 419, 451
AloI GAACNNNNNNTCC 2 cut(s) 275, 307
Alw26I GTCTC 5 cut(s) 730, 1023, 1365, 1531, 1688
AlwI GGATC 2 cut(s) 568, 1424
Ama87I CYCGRG 1 cut(s) 78
AoxI GGCC 6 cut(s) 99, 267, 1168, 1311, 1882, 2101
ApeKI GCWGC 6 cut(s) 551, 581, 794, 1685, 1841, 1844
ApoI RAATTY 5 cut(s) 210, 1157, 1268, 1400, 1582
Asp700I GAANNNNTTC 4 cut(s) 290, 1705, 1734, 2128
AspS9I GGNCC 2 cut(s) 1168, 2234
AsuC2I CCSGG 1 cut(s) 2238
AsuHPI GGTGA 2 cut(s) 491, 785
AvaI CYCGRG 1 cut(s) 78
AvaII GGWCC 1 cut(s) 2234
BalI TGGCCA 1 cut(s) 1313
BanI GGYRCC 1 cut(s) 724
BauI CACGAG 3 cut(s) 1691, 1725, 1748
BbrPI CACGTG 1 cut(s) 302
BbsI GAAGAC 1 cut(s) 182
BbvI GCAGC 6 cut(s) 563, 593, 806, 1697, 1853, 1856
BccI CCATC 6 cut(s) 259, 968, 1218, 1493, 1753, 1838
BceAI ACGGC 2 cut(s) 712, 1005
BciT130I CCWGG 5 cut(s) 271, 408, 704, 1860, 2148
BciVI GTATCC 1 cut(s) 2177
BcnI CCSGG 1 cut(s) 2238
BcoDI GTCTC 5 cut(s) 730, 1023, 1365, 1531, 1688
BfaI CTAG 3 cut(s) 555, 1682, 2163
BfmI CTRYAG 1 cut(s) 564
BfrI CTTAAG 1 cut(s) 1381
BfuI GTATCC 1 cut(s) 2177
BglI GCCNNNNNGGC 1 cut(s) 266
BglII AGATCT 2 cut(s) 106, 640
BisI GCNGC 8 cut(s) 552, 582, 795, 1171, 1211, 1686, 1842, 1845
BlsI GCNGC 8 cut(s) 553, 583, 796, 1172, 1212, 1687, 1843, 1846
Bme1390I CCNGG 6 cut(s) 271, 408, 704, 1860, 2148, 2238
Bme18I GGWCC 1 cut(s) 2234
BmeT110I CYCGRG 1 cut(s) 78
BmgT120I GGNCC 2 cut(s) 1168, 2234
BmiI GGNNCC 2 cut(s) 726, 2139
BmrFI CCNGG 6 cut(s) 271, 408, 704, 1860, 2148, 2238
BmrI ACTGGG 2 cut(s) 493, 2165
BmsI GCATC 2 cut(s) 361, 2160
BmuI ACTGGG 2 cut(s) 493, 2165
BpiI GAAGAC 1 cut(s) 182
BpmI CTGGAG 1 cut(s) 427
Bpu10I CCTNAGC 1 cut(s) 495
BpuEI CTTGAG 1 cut(s) 618
BpuMI CCSGG 1 cut(s) 2238
Bsa29I ATCGAT 1 cut(s) 366
BsaAI YACGTR 1 cut(s) 302
BsaJI CCNNGG 7 cut(s) 189, 270, 1071, 1193, 1280, 1304, 1314
BsaXI ACNNNNNCTCC 4 cut(s) 275, 305, 425, 455
Bsc4I CCNNNNNNNGG 2 cut(s) 1309, 2147
Bse1I ACTGG 3 cut(s) 488, 1130, 2171
Bse3DI GCAATG 3 cut(s) 576, 1655, 2064
BseBI CCWGG 5 cut(s) 271, 408, 704, 1860, 2148
BseCI ATCGAT 1 cut(s) 366
BseDI CCNNGG 7 cut(s) 189, 270, 1071, 1193, 1280, 1304, 1314
BseGI GGATG 3 cut(s) 121, 1522, 2196
BseLI CCNNNNNNNGG 2 cut(s) 1309, 2147
BseMI GCAATG 3 cut(s) 576, 1655, 2064
BseMII CTCAG 2 cut(s) 1503, 1542
BseNI ACTGG 3 cut(s) 488, 1130, 2171
BseRI GAGGAG 2 cut(s) 395, 1041
BseXI GCAGC 6 cut(s) 563, 593, 806, 1697, 1853, 1856
BseYI CCCAGC 1 cut(s) 797
BsgI GTGCAG 1 cut(s) 2231
BshFI GGCC 6 cut(s) 101, 269, 1170, 1313, 1884, 2103
BshNI GGYRCC 1 cut(s) 724
BshVI ATCGAT 1 cut(s) 366
BsiHKCI CYCGRG 1 cut(s) 78
BsiSI CCGG 1 cut(s) 2237
BslFI GGGAC 4 cut(s) 235, 500, 1298, 1801
BslI CCNNNNNNNGG 2 cut(s) 1309, 2147
BsmAI GTCTC 5 cut(s) 730, 1023, 1365, 1531, 1688
BsmBI CGTCTC 1 cut(s) 1023
BsmFI GGGAC 4 cut(s) 235, 500, 1298, 1801
BsmI GAATGC 4 cut(s) 190, 1376, 1395, 1847
BsnI GGCC 6 cut(s) 101, 269, 1170, 1313, 1884, 2103
BsoBI CYCGRG 1 cut(s) 78
Bsp1407I TGTACA 2 cut(s) 1327, 1802
Bsp143I GATC 8 cut(s) 106, 560, 640, 1053, 1347, 1416, 1465, 2085
Bsp19I CCATGG 1 cut(s) 1071
BspACI CCGC 6 cut(s) 219, 253, 672, 1171, 1211, 1446
BspANI GGCC 6 cut(s) 101, 269, 1170, 1313, 1884, 2103
BspCNI CTCAG 2 cut(s) 1502, 1541
BspDI ATCGAT 1 cut(s) 366
BspHI TCATGA 1 cut(s) 1462
BspLI GGNNCC 2 cut(s) 726, 2139
BspPI GGATC 2 cut(s) 568, 1424
BspQI GCTCTTC 5 cut(s) 396, 576, 681, 789, 894
BspT107I GGYRCC 1 cut(s) 724
BspTI CTTAAG 1 cut(s) 1381
BsrBI CCGCTC 1 cut(s) 674
BsrDI GCAATG 3 cut(s) 576, 1655, 2064
BsrGI TGTACA 2 cut(s) 1327, 1802
BsrI ACTGG 3 cut(s) 488, 1130, 2171
BssECI CCNNGG 7 cut(s) 189, 270, 1071, 1193, 1280, 1304, 1314
BssMI GATC 8 cut(s) 106, 560, 640, 1053, 1347, 1416, 1465, 2085
BssSI CACGAG 3 cut(s) 1691, 1725, 1748
BssT1I CCWWGG 3 cut(s) 189, 1071, 1304
Bst2BI CACGAG 3 cut(s) 1691, 1725, 1748
Bst2UI CCWGG 5 cut(s) 271, 408, 704, 1860, 2148
Bst4CI ACNGT 8 cut(s) 16, 291, 422, 1040, 1318, 1327, 1807, 2265
Bst6I CTCTTC 8 cut(s) 141, 330, 396, 576, 681, 789, 894, 2001
BstAFI CTTAAG 1 cut(s) 1381
BstAUI TGTACA 2 cut(s) 1327, 1802
BstBAI YACGTR 1 cut(s) 302
BstC8I GCNNGC 3 cut(s) 7, 376, 1144
BstDEI CTNAG 4 cut(s) 495, 533, 1489, 1528
BstDSI CCRYGG 4 cut(s) 1071, 1193, 1280, 1314
BstENI CCTNNNNNAGG 1 cut(s) 2145
BstF5I GGATG 3 cut(s) 121, 1522, 2196
BstKTI GATC 8 cut(s) 109, 563, 643, 1056, 1350, 1419, 1468, 2088
BstMAI GTCTC 5 cut(s) 730, 1023, 1365, 1531, 1688
BstMBI GATC 8 cut(s) 106, 560, 640, 1053, 1347, 1416, 1465, 2085
BstMWI GCNNNNNNNGC 2 cut(s) 200, 266
BstNI CCWGG 5 cut(s) 271, 408, 704, 1860, 2148
BstNSI RCATGY 4 cut(s) 203, 599, 917, 1150
BstSCI CCNGG 6 cut(s) 269, 406, 702, 1858, 2146, 2236
BstSFI CTRYAG 1 cut(s) 564
BstV1I GCAGC 6 cut(s) 563, 593, 806, 1697, 1853, 1856
BstV2I GAAGAC 1 cut(s) 182
BstX2I RGATCY 4 cut(s) 106, 560, 640, 1416
BstYI RGATCY 4 cut(s) 106, 560, 640, 1416
Bsu15I ATCGAT 1 cut(s) 366
BsuI GTATCC 1 cut(s) 2177
BsuRI GGCC 6 cut(s) 101, 269, 1170, 1313, 1884, 2103
BsuTUI ATCGAT 1 cut(s) 366
BtgI CCRYGG 4 cut(s) 1071, 1193, 1280, 1314
BtsCI GGATG 3 cut(s) 121, 1522, 2196
BtsIMutI CAGTG 3 cut(s) 1045, 2178, 2270
Cac8I GCNNGC 3 cut(s) 7, 376, 1144
CciI TCATGA 1 cut(s) 1462
Cfr13I GGNCC 2 cut(s) 1168, 2234
ClaI ATCGAT 1 cut(s) 366
CseI GACGC 1 cut(s) 716
Csp6I GTAC 7 cut(s) 530, 689, 1036, 1328, 1506, 1803, 1875
CviQI GTAC 7 cut(s) 530, 689, 1036, 1328, 1506, 1803, 1875
DdeI CTNAG 4 cut(s) 495, 533, 1489, 1528
DpnI GATC 8 cut(s) 108, 562, 642, 1055, 1349, 1418, 1467, 2087
DpnII GATC 8 cut(s) 106, 560, 640, 1053, 1347, 1416, 1465, 2085
EaeI YGGCCR 2 cut(s) 99, 1311
Eam1104I CTCTTC 8 cut(s) 141, 330, 396, 576, 681, 789, 894, 2001
EarI CTCTTC 8 cut(s) 141, 330, 396, 576, 681, 789, 894, 2001
Eco130I CCWWGG 3 cut(s) 189, 1071, 1304
Eco147I AGGCCT 1 cut(s) 1884
Eco32I GATATC 1 cut(s) 350
Eco47I GGWCC 1 cut(s) 2234
Eco57I CTGAAG 3 cut(s) 1692, 1914, 1947
Eco72I CACGTG 1 cut(s) 302
Eco88I CYCGRG 1 cut(s) 78
EcoNI CCTNNNNNAGG 1 cut(s) 2145
EcoRII CCWGG 5 cut(s) 269, 406, 702, 1858, 2146
EcoRV GATATC 1 cut(s) 350
EcoT14I CCWWGG 3 cut(s) 189, 1071, 1304
ErhI CCWWGG 3 cut(s) 189, 1071, 1304
Esp3I CGTCTC 1 cut(s) 1023
FaqI GGGAC 4 cut(s) 235, 500, 1298, 1801
FauI CCCGC 3 cut(s) 212, 260, 1453
Fnu4HI GCNGC 8 cut(s) 552, 582, 795, 1171, 1211, 1686, 1842, 1845
FokI GGATG 3 cut(s) 128, 1529, 2203
Fsp4HI GCNGC 8 cut(s) 552, 582, 795, 1171, 1211, 1686, 1842, 1845
FspBI CTAG 3 cut(s) 555, 1682, 2163
GluI GCNGC 8 cut(s) 552, 582, 795, 1171, 1211, 1686, 1842, 1845
GsaI CCCAGC 1 cut(s) 801
GsuI CTGGAG 1 cut(s) 427
HaeIII GGCC 6 cut(s) 101, 269, 1170, 1313, 1884, 2103
HapII CCGG 1 cut(s) 2237
HgaI GACGC 1 cut(s) 716
HincII GTYRAC 2 cut(s) 124, 387
HindII GTYRAC 2 cut(s) 124, 387
HindIII AAGCTT 2 cut(s) 957, 1200
HinfI GANTC 7 cut(s) 223, 440, 464, 631, 1696, 1744, 1983
HpaII CCGG 1 cut(s) 2237
HphI GGTGA 2 cut(s) 491, 785
Hpy166II GTNNAC 4 cut(s) 124, 387, 1541, 1730
Hpy188I TCNGA 5 cut(s) 417, 1347, 1492, 1711, 1933
Hpy188III TCNNGA 8 cut(s) 137, 635, 644, 904, 1463, 1666, 1727, 1748
Hpy8I GTNNAC 4 cut(s) 124, 387, 1541, 1730
HpyAV CCTTC 5 cut(s) 584, 688, 1037, 1258, 1895
HpyCH4III ACNGT 8 cut(s) 16, 291, 422, 1040, 1318, 1327, 1807, 2265
HpyCH4IV ACGT 3 cut(s) 301, 313, 1996
HpyF10VI GCNNNNNNNGC 2 cut(s) 200, 266
HpyF3I CTNAG 4 cut(s) 495, 533, 1489, 1528
HpySE526I ACGT 3 cut(s) 301, 313, 1996
Kzo9I GATC 8 cut(s) 106, 560, 640, 1053, 1347, 1416, 1465, 2085
LguI GCTCTTC 5 cut(s) 396, 576, 681, 789, 894
LmnI GCTCC 3 cut(s) 1046, 1259, 1447
Lsp1109I GCAGC 6 cut(s) 563, 593, 806, 1697, 1853, 1856
LweI GCATC 2 cut(s) 361, 2160
MaeI CTAG 3 cut(s) 555, 1682, 2163
MaeII ACGT 3 cut(s) 301, 313, 1996
MaeIII GTNAC 5 cut(s) 302, 479, 1186, 1825, 2173
MalI GATC 8 cut(s) 108, 562, 642, 1055, 1349, 1418, 1467, 2087
MbiI CCGCTC 1 cut(s) 674
MboI GATC 8 cut(s) 106, 560, 640, 1053, 1347, 1416, 1465, 2085
MfeI CAATTG 3 cut(s) 30, 877, 1163
MflI RGATCY 4 cut(s) 106, 560, 640, 1416
MlsI TGGCCA 1 cut(s) 1313
MluNI TGGCCA 1 cut(s) 1313
MlyI GAGTC 4 cut(s) 217, 434, 625, 1690
MmeI TCCRAC 2 cut(s) 450, 897
Mox20I TGGCCA 1 cut(s) 1313
MroXI GAANNNNTTC 4 cut(s) 290, 1705, 1734, 2128
MscI TGGCCA 1 cut(s) 1313
MseI TTAA 9 cut(s) 48, 207, 1065, 1154, 1205, 1382, 1557, 1656, 2054
MslI CAYNNNNRTG 3 cut(s) 2036, 2081, 2185
Msp20I TGGCCA 1 cut(s) 1313
MspA1I CMGCKG 3 cut(s) 395, 797, 1532
MspCI CTTAAG 1 cut(s) 1381
MspI CCGG 1 cut(s) 2237
MspR9I CCNGG 6 cut(s) 271, 408, 704, 1860, 2148, 2238
MunI CAATTG 3 cut(s) 30, 877, 1163
Mva1269I GAATGC 4 cut(s) 190, 1376, 1395, 1847
MvaI CCWGG 5 cut(s) 271, 408, 704, 1860, 2148
MwoI GCNNNNNNNGC 2 cut(s) 200, 266
NciI CCSGG 1 cut(s) 2238
NcoI CCATGG 1 cut(s) 1071
NdeII GATC 8 cut(s) 106, 560, 640, 1053, 1347, 1416, 1465, 2085
NlaIV GGNNCC 2 cut(s) 726, 2139
NmeAIII GCCGAG 1 cut(s) 1077
NmuCI GTSAC 3 cut(s) 302, 479, 2173
NspI RCATGY 4 cut(s) 203, 599, 917, 1150
PagI TCATGA 1 cut(s) 1462
PceI AGGCCT 1 cut(s) 1884
PciSI GCTCTTC 5 cut(s) 396, 576, 681, 789, 894
PctI GAATGC 4 cut(s) 190, 1376, 1395, 1847
PdmI GAANNNNTTC 4 cut(s) 290, 1705, 1734, 2128
PfeI GAWTC 3 cut(s) 464, 1744, 1983
PkrI GCNGC 8 cut(s) 553, 583, 796, 1172, 1212, 1687, 1843, 1846
PleI GAGTC 4 cut(s) 217, 434, 625, 1690
PmaCI CACGTG 1 cut(s) 302
PmlI CACGTG 1 cut(s) 302
PpsI GAGTC 4 cut(s) 217, 434, 625, 1690
Ppu21I YACGTR 1 cut(s) 302
Psp6I CCWGG 5 cut(s) 269, 406, 702, 1858, 2146
PspCI CACGTG 1 cut(s) 302
PspFI CCCAGC 1 cut(s) 797
PspGI CCWGG 5 cut(s) 269, 406, 702, 1858, 2146
PspN4I GGNNCC 2 cut(s) 726, 2139
PspPI GGNCC 2 cut(s) 1168, 2234
PsuI RGATCY 4 cut(s) 106, 560, 640, 1416
PvuII CAGCTG 3 cut(s) 395, 797, 1532
RsaI GTAC 7 cut(s) 531, 690, 1037, 1329, 1507, 1804, 1876
RsaNI GTAC 7 cut(s) 530, 689, 1036, 1328, 1506, 1803, 1875
RseI CAYNNNNRTG 3 cut(s) 2036, 2081, 2185
SapI GCTCTTC 5 cut(s) 396, 576, 681, 789, 894
SaqAI TTAA 9 cut(s) 48, 207, 1065, 1154, 1205, 1382, 1557, 1656, 2054
SatI GCNGC 8 cut(s) 552, 582, 795, 1171, 1211, 1686, 1842, 1845
Sau3AI GATC 8 cut(s) 106, 560, 640, 1053, 1347, 1416, 1465, 2085
Sau96I GGNCC 2 cut(s) 1168, 2234
SchI GAGTC 4 cut(s) 217, 434, 625, 1690
ScrFI CCNGG 6 cut(s) 271, 408, 704, 1860, 2148, 2238
SfaNI GCATC 2 cut(s) 361, 2160
SfcI CTRYAG 1 cut(s) 564
SinI GGWCC 1 cut(s) 2234
SmiMI CAYNNNNRTG 3 cut(s) 2036, 2081, 2185
SmlI CTYRAG 2 cut(s) 633, 1381
SmoI CTYRAG 2 cut(s) 633, 1381
SseBI AGGCCT 1 cut(s) 1884
SsiI CCGC 6 cut(s) 219, 253, 672, 1171, 1211, 1446
SspI AATATT 2 cut(s) 2129, 2244
SspMI CTAG 3 cut(s) 555, 1682, 2163
StuI AGGCCT 1 cut(s) 1884
StyD4I CCNGG 6 cut(s) 269, 406, 702, 1858, 2146, 2236
StyI CCWWGG 3 cut(s) 189, 1071, 1304
TaaI ACNGT 8 cut(s) 16, 291, 422, 1040, 1318, 1327, 1807, 2265
TaiI ACGT 3 cut(s) 304, 316, 1999
TaqI TCGA 5 cut(s) 366, 438, 1056, 1665, 1699
TatI WGTACW 5 cut(s) 529, 688, 1035, 1327, 1802
TauI GCSGC 2 cut(s) 1173, 1213
TfiI GAWTC 3 cut(s) 464, 1744, 1983
Tru1I TTAA 9 cut(s) 48, 207, 1065, 1154, 1205, 1382, 1557, 1656, 2054
Tru9I TTAA 9 cut(s) 48, 207, 1065, 1154, 1205, 1382, 1557, 1656, 2054
TscAI CASTG 3 cut(s) 1045, 2178, 2270
TseFI GTSAC 3 cut(s) 302, 479, 2173
TseI GCWGC 6 cut(s) 551, 581, 794, 1685, 1841, 1844
Tsp45I GTSAC 3 cut(s) 302, 479, 2173
TspGWI ACGGA 2 cut(s) 1174, 1269
TspRI CASTG 3 cut(s) 1045, 2178, 2270
Vha464I CTTAAG 1 cut(s) 1381
VpaK11BI GGWCC 1 cut(s) 2234
XagI CCTNNNNNAGG 1 cut(s) 2145
XapI RAATTY 5 cut(s) 210, 1157, 1268, 1400, 1582
XceI RCATGY 4 cut(s) 203, 599, 917, 1150
XmnI GAANNNNTTC 4 cut(s) 290, 1705, 1734, 2128
XspI CTAG 3 cut(s) 555, 1682, 2163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.