Domain of unknown function
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 116 |
| AccB1I | GGYRCC | 1 cut(s) | 95 |
| AccII | CGCG | 2 cut(s) | 230, 284 |
| AccIII | TCCGGA | 1 cut(s) | 262 |
| AciI | CCGC | 5 cut(s) | 137, 230, 284, 290, 363 |
| AclWI | GGATC | 3 cut(s) | 165, 165, 324 |
| AcsI | RAATTY | 2 cut(s) | 325, 408 |
| AcyI | GRCGYC | 1 cut(s) | 96 |
| AfaI | GTAC | 1 cut(s) | 21 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 51, 419 |
| AlwI | GGATC | 3 cut(s) | 165, 165, 324 |
| Ama87I | CYCGRG | 1 cut(s) | 180 |
| Aor13HI | TCCGGA | 1 cut(s) | 262 |
| AoxI | GGCC | 3 cut(s) | 205, 267, 402 |
| ApeKI | GCWGC | 4 cut(s) | 82, 104, 140, 165 |
| ApoI | RAATTY | 2 cut(s) | 325, 408 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 206, 238 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 329 |
| AspLEI | GCGC | 2 cut(s) | 98, 127 |
| AspS9I | GGNCC | 3 cut(s) | 11, 205, 402 |
| AsuHPI | GGTGA | 1 cut(s) | 74 |
| AvaI | CYCGRG | 1 cut(s) | 180 |
| AvaII | GGWCC | 1 cut(s) | 11 |
| BanI | GGYRCC | 1 cut(s) | 95 |
| BanII | GRGCYC | 1 cut(s) | 57 |
| BbvI | GCAGC | 4 cut(s) | 94, 116, 152, 177 |
| BccI | CCATC | 2 cut(s) | 342, 421 |
| BceAI | ACGGC | 3 cut(s) | 83, 109, 116 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 269, 303 |
| BclI | TGATCA | 1 cut(s) | 454 |
| BfmI | CTRYAG | 1 cut(s) | 189 |
| BfoI | RGCGCY | 2 cut(s) | 99, 128 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 137 |
| BisI | GCNGC | 6 cut(s) | 83, 105, 138, 141, 166, 363 |
| BlsI | GCNGC | 6 cut(s) | 84, 106, 139, 142, 167, 364 |
| Bme18I | GGWCC | 1 cut(s) | 11 |
| BmeT110I | CYCGRG | 1 cut(s) | 180 |
| BmgT120I | GGNCC | 3 cut(s) | 11, 205, 402 |
| BmiI | GGNNCC | 2 cut(s) | 97, 261 |
| BmsI | GCATC | 1 cut(s) | 349 |
| BpuEI | CTTGAG | 3 cut(s) | 31, 221, 228 |
| Bsa29I | ATCGAT | 1 cut(s) | 319 |
| BsaBI | GATNNNNATC | 1 cut(s) | 175 |
| BsaHI | GRCGYC | 1 cut(s) | 96 |
| BsaWI | WCCGGW | 2 cut(s) | 16, 262 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 51, 419 |
| Bse3DI | GCAATG | 1 cut(s) | 372 |
| Bse8I | GATNNNNATC | 1 cut(s) | 175 |
| BseAI | TCCGGA | 1 cut(s) | 262 |
| BseCI | ATCGAT | 1 cut(s) | 319 |
| BseGI | GGATG | 1 cut(s) | 364 |
| BseJI | GATNNNNATC | 1 cut(s) | 175 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 51, 419 |
| BseMI | GCAATG | 1 cut(s) | 372 |
| BseRI | GAGGAG | 1 cut(s) | 263 |
| BseXI | GCAGC | 4 cut(s) | 94, 116, 152, 177 |
| BsgI | GTGCAG | 1 cut(s) | 509 |
| Bsh1236I | CGCG | 2 cut(s) | 230, 284 |
| Bsh1285I | CGRYCG | 1 cut(s) | 323 |
| BshFI | GGCC | 3 cut(s) | 207, 269, 404 |
| BshNI | GGYRCC | 1 cut(s) | 95 |
| BshVI | ATCGAT | 1 cut(s) | 319 |
| BsiEI | CGRYCG | 1 cut(s) | 323 |
| BsiHKCI | CYCGRG | 1 cut(s) | 180 |
| BsiSI | CCGG | 2 cut(s) | 17, 263 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 51, 419 |
| BsnI | GGCC | 3 cut(s) | 207, 269, 404 |
| BsoBI | CYCGRG | 1 cut(s) | 180 |
| Bsp1286I | GDGCHC | 1 cut(s) | 57 |
| Bsp13I | TCCGGA | 1 cut(s) | 262 |
| Bsp143I | GATC | 6 cut(s) | 157, 170, 176, 316, 320, 454 |
| BspACI | CCGC | 5 cut(s) | 137, 230, 284, 290, 363 |
| BspANI | GGCC | 3 cut(s) | 207, 269, 404 |
| BspDI | ATCGAT | 1 cut(s) | 319 |
| BspEI | TCCGGA | 1 cut(s) | 262 |
| BspFNI | CGCG | 2 cut(s) | 230, 284 |
| BspLI | GGNNCC | 2 cut(s) | 97, 261 |
| BspPI | GGATC | 3 cut(s) | 165, 165, 324 |
| BspT107I | GGYRCC | 1 cut(s) | 95 |
| BsrDI | GCAATG | 1 cut(s) | 372 |
| BssMI | GATC | 6 cut(s) | 157, 170, 176, 316, 320, 454 |
| BssNI | GRCGYC | 1 cut(s) | 96 |
| Bst4CI | ACNGT | 1 cut(s) | 218 |
| BstACI | GRCGYC | 1 cut(s) | 96 |
| BstC8I | GCNNGC | 2 cut(s) | 57, 129 |
| BstF5I | GGATG | 1 cut(s) | 364 |
| BstFNI | CGCG | 2 cut(s) | 230, 284 |
| BstH2I | RGCGCY | 2 cut(s) | 99, 128 |
| BstHHI | GCGC | 2 cut(s) | 98, 127 |
| BstKTI | GATC | 6 cut(s) | 160, 173, 179, 319, 323, 457 |
| BstMBI | GATC | 6 cut(s) | 157, 170, 176, 316, 320, 454 |
| BstMCI | CGRYCG | 1 cut(s) | 323 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 104, 137, 194 |
| BstSFI | CTRYAG | 1 cut(s) | 189 |
| BstUI | CGCG | 2 cut(s) | 230, 284 |
| BstV1I | GCAGC | 4 cut(s) | 94, 116, 152, 177 |
| BstX2I | RGATCY | 1 cut(s) | 170 |
| BstYI | RGATCY | 1 cut(s) | 170 |
| Bsu15I | ATCGAT | 1 cut(s) | 319 |
| BsuRI | GGCC | 3 cut(s) | 207, 269, 404 |
| BsuTUI | ATCGAT | 1 cut(s) | 319 |
| BtsCI | GGATG | 1 cut(s) | 364 |
| Cac8I | GCNNGC | 2 cut(s) | 57, 129 |
| CfoI | GCGC | 2 cut(s) | 98, 127 |
| Cfr13I | GGNCC | 3 cut(s) | 11, 205, 402 |
| ClaI | ATCGAT | 1 cut(s) | 319 |
| CseI | GACGC | 1 cut(s) | 290 |
| Csp6I | GTAC | 1 cut(s) | 20 |
| CviAII | CATG | 3 cut(s) | 424, 439, 466 |
| CviJI | RGCY | 8 cut(s) | 55, 72, 107, 188, 207, 242, 269, 404 |
| CviKI_1 | RGCY | 8 cut(s) | 55, 72, 107, 188, 207, 242, 269, 404 |
| CviQI | GTAC | 1 cut(s) | 20 |
| DinI | GGCGCC | 1 cut(s) | 97 |
| DpnI | GATC | 6 cut(s) | 159, 172, 178, 318, 322, 456 |
| DpnII | GATC | 6 cut(s) | 157, 170, 176, 316, 320, 454 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 116 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 116 |
| Eco24I | GRGCYC | 1 cut(s) | 57 |
| Eco32I | GATATC | 1 cut(s) | 386 |
| Eco47I | GGWCC | 1 cut(s) | 11 |
| Eco88I | CYCGRG | 1 cut(s) | 180 |
| EcoO109I | RGGNCCY | 1 cut(s) | 205 |
| EcoRI | GAATTC | 1 cut(s) | 408 |
| EcoRV | GATATC | 1 cut(s) | 386 |
| EcoT38I | GRGCYC | 1 cut(s) | 57 |
| EgeI | GGCGCC | 1 cut(s) | 97 |
| EheI | GGCGCC | 1 cut(s) | 97 |
| FaeI | CATG | 3 cut(s) | 427, 442, 469 |
| FaiI | YATR | 5 cut(s) | 425, 440, 453, 467, 520 |
| FatI | CATG | 3 cut(s) | 423, 438, 465 |
| FbaI | TGATCA | 1 cut(s) | 454 |
| Fnu4HI | GCNGC | 6 cut(s) | 83, 105, 138, 141, 166, 363 |
| FokI | GGATG | 1 cut(s) | 371 |
| FriOI | GRGCYC | 1 cut(s) | 57 |
| Fsp4HI | GCNGC | 6 cut(s) | 83, 105, 138, 141, 166, 363 |
| GlaI | GCGC | 2 cut(s) | 97, 126 |
| GluI | GCNGC | 6 cut(s) | 83, 105, 138, 141, 166, 363 |
| HaeII | RGCGCY | 2 cut(s) | 99, 128 |
| HaeIII | GGCC | 3 cut(s) | 207, 269, 404 |
| HapII | CCGG | 2 cut(s) | 17, 263 |
| HgaI | GACGC | 1 cut(s) | 290 |
| HhaI | GCGC | 2 cut(s) | 98, 127 |
| Hin1I | GRCGYC | 1 cut(s) | 96 |
| Hin1II | CATG | 3 cut(s) | 427, 442, 469 |
| Hin6I | GCGC | 2 cut(s) | 96, 125 |
| HinP1I | GCGC | 2 cut(s) | 96, 125 |
| HinfI | GANTC | 2 cut(s) | 145, 376 |
| HpaII | CCGG | 2 cut(s) | 17, 263 |
| HphI | GGTGA | 1 cut(s) | 74 |
| Hpy166II | GTNNAC | 1 cut(s) | 65 |
| Hpy188I | TCNGA | 2 cut(s) | 150, 157 |
| Hpy188III | TCNNGA | 6 cut(s) | 174, 180, 182, 245, 263, 350 |
| Hpy8I | GTNNAC | 1 cut(s) | 65 |
| Hpy99I | CGWCG | 6 cut(s) | 79, 113, 215, 218, 275, 284 |
| HpyCH4III | ACNGT | 1 cut(s) | 218 |
| HpyCH4IV | ACGT | 3 cut(s) | 77, 111, 273 |
| HpyCH4V | TGCA | 4 cut(s) | 4, 82, 104, 490 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 104, 137, 194 |
| HpySE526I | ACGT | 3 cut(s) | 77, 111, 273 |
| Hsp92I | GRCGYC | 1 cut(s) | 96 |
| Hsp92II | CATG | 3 cut(s) | 427, 442, 469 |
| HspAI | GCGC | 2 cut(s) | 96, 125 |
| KasI | GGCGCC | 1 cut(s) | 95 |
| Kpn2I | TCCGGA | 1 cut(s) | 262 |
| Ksp22I | TGATCA | 1 cut(s) | 454 |
| Kzo9I | GATC | 6 cut(s) | 157, 170, 176, 316, 320, 454 |
| LpnPI | CCDG | 5 cut(s) | 30, 45, 276, 405, 418 |
| Lsp1109I | GCAGC | 4 cut(s) | 94, 116, 152, 177 |
| LweI | GCATC | 1 cut(s) | 349 |
| MaeII | ACGT | 3 cut(s) | 77, 111, 273 |
| MalI | GATC | 6 cut(s) | 159, 172, 178, 318, 322, 456 |
| MboI | GATC | 6 cut(s) | 157, 170, 176, 316, 320, 454 |
| MboII | GAAGA | 2 cut(s) | 322, 425 |
| MflI | RGATCY | 1 cut(s) | 170 |
| MhlI | GDGCHC | 1 cut(s) | 57 |
| MluCI | AATT | 6 cut(s) | 5, 39, 325, 408, 429, 482 |
| Mly113I | GGCGCC | 1 cut(s) | 96 |
| MmeI | TCCRAC | 1 cut(s) | 173 |
| MroI | TCCGGA | 1 cut(s) | 262 |
| MroXI | GAANNNNTTC | 1 cut(s) | 329 |
| MseI | TTAA | 1 cut(s) | 395 |
| MspI | CCGG | 2 cut(s) | 17, 263 |
| MvnI | CGCG | 2 cut(s) | 230, 284 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 104, 137, 194 |
| NarI | GGCGCC | 1 cut(s) | 96 |
| NdeII | GATC | 6 cut(s) | 157, 170, 176, 316, 320, 454 |
| NlaIII | CATG | 3 cut(s) | 427, 442, 469 |
| NlaIV | GGNNCC | 2 cut(s) | 97, 261 |
| PaeR7I | CTCGAG | 1 cut(s) | 180 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 117 |
| PdmI | GAANNNNTTC | 1 cut(s) | 329 |
| PfeI | GAWTC | 2 cut(s) | 145, 376 |
| PkrI | GCNGC | 6 cut(s) | 84, 106, 139, 142, 167, 364 |
| Ple19I | CGATCG | 1 cut(s) | 323 |
| PluTI | GGCGCC | 1 cut(s) | 99 |
| PspN4I | GGNNCC | 2 cut(s) | 97, 261 |
| PspPI | GGNCC | 3 cut(s) | 11, 205, 402 |
| PsuI | RGATCY | 1 cut(s) | 170 |
| PvuI | CGATCG | 1 cut(s) | 323 |
| RsaI | GTAC | 1 cut(s) | 21 |
| RsaNI | GTAC | 1 cut(s) | 20 |
| SaqAI | TTAA | 1 cut(s) | 395 |
| SatI | GCNGC | 6 cut(s) | 83, 105, 138, 141, 166, 363 |
| Sau3AI | GATC | 6 cut(s) | 157, 170, 176, 316, 320, 454 |
| Sau96I | GGNCC | 3 cut(s) | 11, 205, 402 |
| SduI | GDGCHC | 1 cut(s) | 57 |
| SetI | ASST | 7 cut(s) | 16, 80, 114, 118, 255, 261, 276 |
| SfaNI | GCATC | 1 cut(s) | 349 |
| SfcI | CTRYAG | 1 cut(s) | 189 |
| SfoI | GGCGCC | 1 cut(s) | 97 |
| Sfr274I | CTCGAG | 1 cut(s) | 180 |
| SinI | GGWCC | 1 cut(s) | 11 |
| SlaI | CTCGAG | 1 cut(s) | 180 |
| SmlI | CTYRAG | 4 cut(s) | 46, 180, 236, 243 |
| SmoI | CTYRAG | 4 cut(s) | 46, 180, 236, 243 |
| Sse9I | AATT | 6 cut(s) | 5, 39, 325, 408, 429, 482 |
| SsiI | CCGC | 5 cut(s) | 137, 230, 284, 290, 363 |
| SspDI | GGCGCC | 1 cut(s) | 95 |
| TaaI | ACNGT | 1 cut(s) | 218 |
| TaiI | ACGT | 3 cut(s) | 80, 114, 276 |
| TaqI | TCGA | 5 cut(s) | 120, 181, 210, 319, 323 |
| TasI | AATT | 6 cut(s) | 5, 39, 325, 408, 429, 482 |
| TauI | GCSGC | 2 cut(s) | 140, 365 |
| TfiI | GAWTC | 2 cut(s) | 145, 376 |
| Tru1I | TTAA | 1 cut(s) | 395 |
| Tru9I | TTAA | 1 cut(s) | 395 |
| TseI | GCWGC | 4 cut(s) | 82, 104, 140, 165 |
| TspDTI | ATGAA | 2 cut(s) | 495, 512 |
| VpaK11BI | GGWCC | 1 cut(s) | 11 |
| XapI | RAATTY | 2 cut(s) | 325, 408 |
| XhoI | CTCGAG | 1 cut(s) | 180 |
| XmnI | GAANNNNTTC | 1 cut(s) | 329 |