Domain of unknown function (DUF4219)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 1121, 1266 |
| AarI | CACCTGC | 1 cut(s) | 806 |
| AatII | GACGTC | 1 cut(s) | 1233 |
| Acc36I | ACCTGC | 2 cut(s) | 281, 806 |
| AccBSI | CCGCTC | 2 cut(s) | 596, 929 |
| AciI | CCGC | 6 cut(s) | 216, 383, 594, 646, 927, 1330 |
| AclWI | GGATC | 1 cut(s) | 911 |
| AcuI | CTGAAG | 1 cut(s) | 1035 |
| AcyI | GRCGYC | 1 cut(s) | 1230 |
| AfaI | GTAC | 2 cut(s) | 724, 1057 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1003 |
| AgsI | TTSAA | 7 cut(s) | 389, 401, 458, 734, 794, 1042, 1307 |
| AjnI | CCWGG | 2 cut(s) | 167, 266 |
| AleI | CACNNNNGTG | 1 cut(s) | 1354 |
| AluBI | AGCT | 8 cut(s) | 98, 236, 431, 779, 808, 851, 1002, 1087 |
| AluI | AGCT | 8 cut(s) | 98, 236, 431, 779, 808, 851, 1002, 1087 |
| Alw26I | GTCTC | 2 cut(s) | 110, 329 |
| AlwI | GGATC | 1 cut(s) | 911 |
| Ama87I | CYCGRG | 1 cut(s) | 575 |
| AoxI | GGCC | 2 cut(s) | 165, 1276 |
| ApeKI | GCWGC | 2 cut(s) | 5, 524 |
| AsuHPI | GGTGA | 2 cut(s) | 323, 725 |
| AsuII | TTCGAA | 1 cut(s) | 536 |
| AvaI | CYCGRG | 1 cut(s) | 575 |
| AxyI | CCTNAGG | 1 cut(s) | 307 |
| BbsI | GAAGAC | 2 cut(s) | 129, 1023 |
| BbvI | GCAGC | 1 cut(s) | 511 |
| BccI | CCATC | 5 cut(s) | 349, 458, 464, 1151, 1244 |
| BceAI | ACGGC | 2 cut(s) | 658, 877 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 516, 550, 905, 939 |
| BciT130I | CCWGG | 2 cut(s) | 169, 268 |
| BciVI | GTATCC | 1 cut(s) | 1337 |
| BcoDI | GTCTC | 2 cut(s) | 110, 329 |
| BfaI | CTAG | 1 cut(s) | 1134 |
| BfuAI | ACCTGC | 2 cut(s) | 281, 806 |
| BfuI | GTATCC | 1 cut(s) | 1337 |
| BisI | GCNGC | 3 cut(s) | 6, 525, 927 |
| BlpI | GCTNAGC | 2 cut(s) | 132, 1288 |
| BlsI | GCNGC | 3 cut(s) | 7, 526, 928 |
| Bme1390I | CCNGG | 2 cut(s) | 169, 268 |
| BmeT110I | CYCGRG | 1 cut(s) | 575 |
| BmrFI | CCNGG | 2 cut(s) | 169, 268 |
| BmrI | ACTGGG | 2 cut(s) | 5, 15 |
| BmsI | GCATC | 4 cut(s) | 214, 352, 628, 1204 |
| BmuI | ACTGGG | 2 cut(s) | 5, 15 |
| BpiI | GAAGAC | 2 cut(s) | 129, 1023 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 290, 322 |
| BpmI | CTGGAG | 1 cut(s) | 371 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 132, 1288 |
| Bpu14I | TTCGAA | 1 cut(s) | 536 |
| BpuEI | CTTGAG | 1 cut(s) | 1073 |
| BsaAI | YACGTR | 1 cut(s) | 1356 |
| BsaHI | GRCGYC | 1 cut(s) | 1230 |
| BsaI | GGTCTC | 1 cut(s) | 110 |
| BsaJI | CCNNGG | 4 cut(s) | 168, 186, 576, 1173 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1003 |
| Bse1I | ACTGG | 3 cut(s) | 11, 21, 354 |
| Bse21I | CCTNAGG | 1 cut(s) | 307 |
| Bse3DI | GCAATG | 1 cut(s) | 1299 |
| BseBI | CCWGG | 2 cut(s) | 169, 268 |
| BseDI | CCNNGG | 4 cut(s) | 168, 186, 576, 1173 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1003 |
| BseMI | GCAATG | 1 cut(s) | 1299 |
| BseMII | CTCAG | 5 cut(s) | 123, 246, 321, 958, 1302 |
| BseNI | ACTGG | 3 cut(s) | 11, 21, 354 |
| BseRI | GAGGAG | 1 cut(s) | 818 |
| BseXI | GCAGC | 1 cut(s) | 511 |
| BshFI | GGCC | 2 cut(s) | 167, 1278 |
| BsiHKCI | CYCGRG | 1 cut(s) | 575 |
| BsiSI | CCGG | 1 cut(s) | 416 |
| BslFI | GGGAC | 2 cut(s) | 2, 557 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1003 |
| BsmAI | GTCTC | 2 cut(s) | 110, 329 |
| BsmFI | GGGAC | 2 cut(s) | 2, 557 |
| BsmI | GAATGC | 1 cut(s) | 1067 |
| BsnI | GGCC | 2 cut(s) | 167, 1278 |
| Bso31I | GGTCTC | 1 cut(s) | 110 |
| BsoBI | CYCGRG | 1 cut(s) | 575 |
| Bsp119I | TTCGAA | 1 cut(s) | 536 |
| Bsp1407I | TGTACA | 2 cut(s) | 722, 1055 |
| Bsp143I | GATC | 4 cut(s) | 177, 394, 916, 979 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 132, 1288 |
| Bsp19I | CCATGG | 1 cut(s) | 1173 |
| BspACI | CCGC | 6 cut(s) | 216, 383, 594, 646, 927, 1330 |
| BspANI | GGCC | 2 cut(s) | 167, 1278 |
| BspCNI | CTCAG | 5 cut(s) | 124, 245, 320, 957, 1301 |
| BspMI | ACCTGC | 2 cut(s) | 281, 806 |
| BspPI | GGATC | 1 cut(s) | 911 |
| BspT104I | TTCGAA | 1 cut(s) | 536 |
| BspTNI | GGTCTC | 1 cut(s) | 110 |
| BsrBI | CCGCTC | 2 cut(s) | 596, 929 |
| BsrDI | GCAATG | 1 cut(s) | 1299 |
| BsrGI | TGTACA | 2 cut(s) | 722, 1055 |
| BsrI | ACTGG | 3 cut(s) | 11, 21, 354 |
| BssECI | CCNNGG | 4 cut(s) | 168, 186, 576, 1173 |
| BssMI | GATC | 4 cut(s) | 177, 394, 916, 979 |
| BssNI | GRCGYC | 1 cut(s) | 1230 |
| BssT1I | CCWWGG | 1 cut(s) | 1173 |
| Bst2UI | CCWGG | 2 cut(s) | 169, 268 |
| Bst4CI | ACNGT | 5 cut(s) | 485, 619, 666, 713, 740 |
| BstACI | GRCGYC | 1 cut(s) | 1230 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 638 |
| BstAUI | TGTACA | 2 cut(s) | 722, 1055 |
| BstBAI | YACGTR | 1 cut(s) | 1356 |
| BstBI | TTCGAA | 1 cut(s) | 536 |
| BstC8I | GCNNGC | 3 cut(s) | 198, 976, 1085 |
| BstDEI | CTNAG | 5 cut(s) | 132, 232, 307, 944, 1288 |
| BstDSI | CCRYGG | 1 cut(s) | 1173 |
| BstKTI | GATC | 4 cut(s) | 180, 397, 919, 982 |
| BstMAI | GTCTC | 2 cut(s) | 110, 329 |
| BstMBI | GATC | 4 cut(s) | 177, 394, 916, 979 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 197, 233, 263, 287, 638, 1284 |
| BstNI | CCWGG | 2 cut(s) | 169, 268 |
| BstNSI | RCATGY | 2 cut(s) | 200, 1056 |
| BstSCI | CCNGG | 2 cut(s) | 167, 266 |
| BstV1I | GCAGC | 1 cut(s) | 511 |
| BstV2I | GAAGAC | 2 cut(s) | 129, 1023 |
| Bsu36I | CCTNAGG | 1 cut(s) | 307 |
| BsuI | GTATCC | 1 cut(s) | 1337 |
| BsuRI | GGCC | 2 cut(s) | 167, 1278 |
| BtgI | CCRYGG | 1 cut(s) | 1173 |
| BtsIMutI | CAGTG | 1 cut(s) | 498 |
| BveI | ACCTGC | 2 cut(s) | 281, 806 |
| Cac8I | GCNNGC | 3 cut(s) | 198, 976, 1085 |
| CseI | GACGC | 1 cut(s) | 137 |
| Csp6I | GTAC | 2 cut(s) | 723, 1056 |
| CviAII | CATG | 5 cut(s) | 197, 272, 521, 1053, 1174 |
| CviQI | GTAC | 2 cut(s) | 723, 1056 |
| DdeI | CTNAG | 5 cut(s) | 132, 232, 307, 944, 1288 |
| DpnI | GATC | 4 cut(s) | 179, 396, 918, 981 |
| DpnII | GATC | 4 cut(s) | 177, 394, 916, 979 |
| Eco130I | CCWWGG | 1 cut(s) | 1173 |
| Eco147I | AGGCCT | 1 cut(s) | 167 |
| Eco31I | GGTCTC | 1 cut(s) | 110 |
| Eco57I | CTGAAG | 1 cut(s) | 1035 |
| Eco81I | CCTNAGG | 1 cut(s) | 307 |
| Eco88I | CYCGRG | 1 cut(s) | 575 |
| EcoRII | CCWGG | 2 cut(s) | 167, 266 |
| EcoT14I | CCWWGG | 1 cut(s) | 1173 |
| ErhI | CCWWGG | 1 cut(s) | 1173 |
| FaeI | CATG | 5 cut(s) | 200, 275, 524, 1056, 1177 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 381, 413 |
| FaqI | GGGAC | 2 cut(s) | 2, 557 |
| FatI | CATG | 5 cut(s) | 196, 271, 520, 1052, 1173 |
| FauI | CCCGC | 1 cut(s) | 639 |
| Fnu4HI | GCNGC | 3 cut(s) | 6, 525, 927 |
| Fsp4HI | GCNGC | 3 cut(s) | 6, 525, 927 |
| FspBI | CTAG | 1 cut(s) | 1134 |
| GluI | GCNGC | 3 cut(s) | 6, 525, 927 |
| GsuI | CTGGAG | 1 cut(s) | 371 |
| HaeIII | GGCC | 2 cut(s) | 167, 1278 |
| HapII | CCGG | 1 cut(s) | 416 |
| HgaI | GACGC | 1 cut(s) | 137 |
| Hin1I | GRCGYC | 1 cut(s) | 1230 |
| Hin1II | CATG | 5 cut(s) | 200, 275, 524, 1056, 1177 |
| HindIII | AAGCTT | 2 cut(s) | 849, 1000 |
| HinfI | GANTC | 1 cut(s) | 1160 |
| HpaII | CCGG | 1 cut(s) | 416 |
| HphI | GGTGA | 2 cut(s) | 323, 725 |
| Hpy166II | GTNNAC | 1 cut(s) | 1058 |
| Hpy188I | TCNGA | 1 cut(s) | 1027 |
| Hpy188III | TCNNGA | 2 cut(s) | 1147, 1321 |
| Hpy8I | GTNNAC | 1 cut(s) | 1058 |
| Hpy99I | CGWCG | 3 cut(s) | 131, 144, 1235 |
| HpyAV | CCTTC | 4 cut(s) | 238, 383, 452, 1071 |
| HpyCH4III | ACNGT | 5 cut(s) | 485, 619, 666, 713, 740 |
| HpyCH4IV | ACGT | 5 cut(s) | 160, 546, 1116, 1230, 1355 |
| HpyCH4V | TGCA | 8 cut(s) | 196, 200, 489, 527, 632, 815, 1067, 1217 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 197, 233, 263, 287, 638, 1284 |
| HpyF3I | CTNAG | 5 cut(s) | 132, 232, 307, 944, 1288 |
| HpySE526I | ACGT | 5 cut(s) | 160, 546, 1116, 1230, 1355 |
| Hsp92I | GRCGYC | 1 cut(s) | 1230 |
| Hsp92II | CATG | 5 cut(s) | 200, 275, 524, 1056, 1177 |
| Kzo9I | GATC | 4 cut(s) | 177, 394, 916, 979 |
| LmnI | GCTCC | 2 cut(s) | 601, 805 |
| Lsp1109I | GCAGC | 1 cut(s) | 511 |
| LweI | GCATC | 4 cut(s) | 214, 352, 628, 1204 |
| MaeI | CTAG | 1 cut(s) | 1134 |
| MaeII | ACGT | 5 cut(s) | 160, 546, 1116, 1230, 1355 |
| MaeIII | GTNAC | 2 cut(s) | 583, 895 |
| MalI | GATC | 4 cut(s) | 179, 396, 918, 981 |
| MbiI | CCGCTC | 2 cut(s) | 596, 929 |
| MboI | GATC | 4 cut(s) | 177, 394, 916, 979 |
| MboII | GAAGA | 7 cut(s) | 74, 134, 371, 389, 437, 1028, 1259 |
| MluCI | AATT | 6 cut(s) | 201, 810, 1030, 1046, 1218, 1261 |
| MmeI | TCCRAC | 2 cut(s) | 623, 1262 |
| MnlI | CCTC | 9 cut(s) | 147, 157, 181, 292, 316, 571, 796, 953, 1289 |
| MseI | TTAA | 3 cut(s) | 45, 564, 858 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1354 |
| MspI | CCGG | 1 cut(s) | 416 |
| MspR9I | CCNGG | 2 cut(s) | 169, 268 |
| Mva1269I | GAATGC | 1 cut(s) | 1067 |
| MvaI | CCWGG | 2 cut(s) | 169, 268 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 197, 233, 263, 287, 638, 1284 |
| NcoI | CCATGG | 1 cut(s) | 1173 |
| NdeII | GATC | 4 cut(s) | 177, 394, 916, 979 |
| NlaIII | CATG | 5 cut(s) | 200, 275, 524, 1056, 1177 |
| NmeAIII | GCCGAG | 1 cut(s) | 211 |
| NmuCI | GTSAC | 1 cut(s) | 895 |
| NspI | RCATGY | 2 cut(s) | 200, 1056 |
| NspV | TTCGAA | 1 cut(s) | 536 |
| OliI | CACNNNNGTG | 1 cut(s) | 1354 |
| PaeI | GCATGC | 1 cut(s) | 200 |
| PaqCI | CACCTGC | 1 cut(s) | 806 |
| PceI | AGGCCT | 1 cut(s) | 167 |
| PctI | GAATGC | 1 cut(s) | 1067 |
| PfeI | GAWTC | 1 cut(s) | 1160 |
| PkrI | GCNGC | 3 cut(s) | 7, 526, 928 |
| Ppu21I | YACGTR | 1 cut(s) | 1356 |
| PsiI | TTATAA | 2 cut(s) | 1121, 1266 |
| Psp6I | CCWGG | 2 cut(s) | 167, 266 |
| PspGI | CCWGG | 2 cut(s) | 167, 266 |
| RsaI | GTAC | 2 cut(s) | 724, 1057 |
| RsaNI | GTAC | 2 cut(s) | 723, 1056 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1354 |
| SaqAI | TTAA | 3 cut(s) | 45, 564, 858 |
| SatI | GCNGC | 3 cut(s) | 6, 525, 927 |
| Sau3AI | GATC | 4 cut(s) | 177, 394, 916, 979 |
| ScrFI | CCNGG | 2 cut(s) | 169, 268 |
| SfaNI | GCATC | 4 cut(s) | 214, 352, 628, 1204 |
| SfuI | TTCGAA | 1 cut(s) | 536 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1354 |
| SmlI | CTYRAG | 1 cut(s) | 1088 |
| SmoI | CTYRAG | 1 cut(s) | 1088 |
| SphI | GCATGC | 1 cut(s) | 200 |
| Sse9I | AATT | 6 cut(s) | 201, 810, 1030, 1046, 1218, 1261 |
| SseBI | AGGCCT | 1 cut(s) | 167 |
| SsiI | CCGC | 6 cut(s) | 216, 383, 594, 646, 927, 1330 |
| SspMI | CTAG | 1 cut(s) | 1134 |
| StuI | AGGCCT | 1 cut(s) | 167 |
| StyD4I | CCNGG | 2 cut(s) | 167, 266 |
| StyI | CCWWGG | 1 cut(s) | 1173 |
| TaaI | ACNGT | 5 cut(s) | 485, 619, 666, 713, 740 |
| TaiI | ACGT | 5 cut(s) | 163, 549, 1119, 1233, 1358 |
| TaqI | TCGA | 6 cut(s) | 81, 536, 636, 771, 825, 846 |
| TasI | AATT | 6 cut(s) | 201, 810, 1030, 1046, 1218, 1261 |
| TatI | WGTACW | 2 cut(s) | 722, 1055 |
| TauI | GCSGC | 1 cut(s) | 929 |
| TfiI | GAWTC | 1 cut(s) | 1160 |
| Tru1I | TTAA | 3 cut(s) | 45, 564, 858 |
| Tru9I | TTAA | 3 cut(s) | 45, 564, 858 |
| TscAI | CASTG | 1 cut(s) | 505 |
| TseFI | GTSAC | 1 cut(s) | 895 |
| TseI | GCWGC | 2 cut(s) | 5, 524 |
| Tsp45I | GTSAC | 1 cut(s) | 895 |
| TspDTI | ATGAA | 5 cut(s) | 51, 194, 509, 777, 1059 |
| TspGWI | ACGGA | 1 cut(s) | 815 |
| TspRI | CASTG | 1 cut(s) | 505 |
| XceI | RCATGY | 2 cut(s) | 200, 1056 |
| XspI | CTAG | 1 cut(s) | 1134 |
| ZraI | GACGTC | 1 cut(s) | 1231 |