Domain of unknown function
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1017 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 356 |
| AatII | GACGTC | 1 cut(s) | 1833 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1318 |
| AccI | GTMKAC | 2 cut(s) | 600, 1024 |
| AccII | CGCG | 1 cut(s) | 1119 |
| AciI | CCGC | 2 cut(s) | 219, 419 |
| AclI | AACGTT | 1 cut(s) | 409 |
| AclWI | GGATC | 3 cut(s) | 713, 1650, 1880 |
| AcsI | RAATTY | 8 cut(s) | 161, 306, 491, 1227, 1460, 1657, 1685, 1705 |
| AcuI | CTGAAG | 2 cut(s) | 123, 1902 |
| AcyI | GRCGYC | 1 cut(s) | 1830 |
| AdeI | CACNNNGTG | 1 cut(s) | 1679 |
| AfaI | GTAC | 3 cut(s) | 961, 1183, 2109 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 438, 439, 538, 1318 |
| AflII | CTTAAG | 1 cut(s) | 815 |
| AflIII | ACRYGT | 1 cut(s) | 1184 |
| AgeI | ACCGGT | 1 cut(s) | 548 |
| AgsI | TTSAA | 8 cut(s) | 295, 497, 538, 593, 809, 1412, 1612, 1855 |
| AhlI | ACTAGT | 1 cut(s) | 1144 |
| AjiI | CACGTC | 1 cut(s) | 544 |
| AjnI | CCWGG | 3 cut(s) | 269, 472, 1813 |
| Alw26I | GTCTC | 2 cut(s) | 320, 1351 |
| AlwI | GGATC | 3 cut(s) | 713, 1650, 1880 |
| Ama87I | CYCGRG | 1 cut(s) | 343 |
| AoxI | GGCC | 2 cut(s) | 429, 2031 |
| ApeKI | GCWGC | 6 cut(s) | 5, 1522, 1577, 1718, 1769, 1796 |
| ApoI | RAATTY | 8 cut(s) | 161, 306, 491, 1227, 1460, 1657, 1685, 1705 |
| AseI | ATTAAT | 1 cut(s) | 183 |
| AsiGI | ACCGGT | 1 cut(s) | 548 |
| AspLEI | GCGC | 2 cut(s) | 1577, 1626 |
| AspS9I | GGNCC | 4 cut(s) | 430, 826, 1294, 2031 |
| AsuC2I | CCSGG | 1 cut(s) | 433 |
| AsuHPI | GGTGA | 2 cut(s) | 94, 1154 |
| AsuNHI | GCTAGC | 1 cut(s) | 983 |
| AvaI | CYCGRG | 1 cut(s) | 343 |
| AvaII | GGWCC | 2 cut(s) | 826, 1294 |
| AxyI | CCTNAGG | 2 cut(s) | 1056, 1638 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 639, 672 |
| BbsI | GAAGAC | 1 cut(s) | 159 |
| BbvI | GCAGC | 6 cut(s) | 17, 1509, 1589, 1730, 1756, 1783 |
| BccI | CCATC | 8 cut(s) | 91, 119, 602, 742, 1002, 1229, 1609, 1658 |
| BceAI | ACGGC | 1 cut(s) | 978 |
| BciT130I | CCWGG | 3 cut(s) | 271, 474, 1815 |
| BciVI | GTATCC | 1 cut(s) | 640 |
| BcnI | CCSGG | 1 cut(s) | 433 |
| BcoDI | GTCTC | 2 cut(s) | 320, 1351 |
| BcuI | ACTAGT | 1 cut(s) | 1144 |
| BfaI | CTAG | 6 cut(s) | 21, 638, 957, 984, 1145, 2055 |
| BfrI | CTTAAG | 1 cut(s) | 815 |
| BfuI | GTATCC | 1 cut(s) | 640 |
| BisI | GCNGC | 6 cut(s) | 6, 1523, 1578, 1719, 1770, 1797 |
| BlsI | GCNGC | 6 cut(s) | 7, 1524, 1579, 1720, 1771, 1798 |
| Bme1390I | CCNGG | 4 cut(s) | 271, 433, 474, 1815 |
| Bme18I | GGWCC | 2 cut(s) | 826, 1294 |
| BmeT110I | CYCGRG | 1 cut(s) | 343 |
| BmgBI | CACGTC | 1 cut(s) | 544 |
| BmgT120I | GGNCC | 4 cut(s) | 430, 826, 1294, 2031 |
| BmiI | GGNNCC | 3 cut(s) | 827, 1373, 2032 |
| BmrFI | CCNGG | 4 cut(s) | 271, 433, 474, 1815 |
| BmsI | GCATC | 5 cut(s) | 217, 256, 784, 1435, 2133 |
| BmtI | GCTAGC | 1 cut(s) | 987 |
| BpiI | GAAGAC | 1 cut(s) | 159 |
| BpmI | CTGGAG | 2 cut(s) | 303, 1158 |
| BpuEI | CTTGAG | 1 cut(s) | 903 |
| BpuMI | CCSGG | 1 cut(s) | 433 |
| BsaAI | YACGTR | 1 cut(s) | 1187 |
| BsaHI | GRCGYC | 1 cut(s) | 1830 |
| BsaI | GGTCTC | 1 cut(s) | 1351 |
| BsaJI | CCNNGG | 3 cut(s) | 473, 1367, 1860 |
| BsaWI | WCCGGW | 1 cut(s) | 548 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1131, 1161 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 438, 439, 538, 1318 |
| Bse118I | RCCGGY | 1 cut(s) | 548 |
| Bse1I | ACTGG | 2 cut(s) | 74, 532 |
| Bse21I | CCTNAGG | 2 cut(s) | 1056, 1638 |
| BseBI | CCWGG | 3 cut(s) | 271, 474, 1815 |
| BseDI | CCNNGG | 3 cut(s) | 473, 1367, 1860 |
| BseGI | GGATG | 3 cut(s) | 121, 1976, 2148 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 438, 439, 538, 1318 |
| BseMII | CTCAG | 2 cut(s) | 222, 1047 |
| BseNI | ACTGG | 2 cut(s) | 74, 532 |
| BseRI | GAGGAG | 1 cut(s) | 609 |
| BseXI | GCAGC | 6 cut(s) | 17, 1509, 1589, 1730, 1756, 1783 |
| Bsh1236I | CGCG | 1 cut(s) | 1119 |
| BshFI | GGCC | 2 cut(s) | 431, 2033 |
| BshTI | ACCGGT | 1 cut(s) | 548 |
| BsiHKCI | CYCGRG | 1 cut(s) | 343 |
| BsiSI | CCGG | 3 cut(s) | 433, 549, 930 |
| BslFI | GGGAC | 2 cut(s) | 2, 839 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 438, 439, 538, 1318 |
| BsmAI | GTCTC | 2 cut(s) | 320, 1351 |
| BsmBI | CGTCTC | 1 cut(s) | 320 |
| BsmFI | GGGAC | 2 cut(s) | 2, 839 |
| BsnI | GGCC | 2 cut(s) | 431, 2033 |
| Bso31I | GGTCTC | 1 cut(s) | 1351 |
| BsoBI | CYCGRG | 1 cut(s) | 343 |
| Bsp143I | GATC | 6 cut(s) | 718, 1090, 1267, 1380, 1642, 1872 |
| Bsp68I | TCGCGA | 1 cut(s) | 1119 |
| BspACI | CCGC | 2 cut(s) | 219, 419 |
| BspANI | GGCC | 2 cut(s) | 431, 2033 |
| BspCNI | CTCAG | 2 cut(s) | 223, 1048 |
| BspFNI | CGCG | 1 cut(s) | 1119 |
| BspLI | GGNNCC | 3 cut(s) | 827, 1373, 2032 |
| BspOI | GCTAGC | 1 cut(s) | 987 |
| BspPI | GGATC | 3 cut(s) | 713, 1650, 1880 |
| BspQI | GCTCTTC | 2 cut(s) | 522, 1912 |
| BspTI | CTTAAG | 1 cut(s) | 815 |
| BspTNI | GGTCTC | 1 cut(s) | 1351 |
| BsrFI | RCCGGY | 1 cut(s) | 548 |
| BsrI | ACTGG | 2 cut(s) | 74, 532 |
| BssAI | RCCGGY | 1 cut(s) | 548 |
| BssECI | CCNNGG | 3 cut(s) | 473, 1367, 1860 |
| BssMI | GATC | 6 cut(s) | 718, 1090, 1267, 1380, 1642, 1872 |
| BssNAI | GTATAC | 1 cut(s) | 1025 |
| BssNI | GRCGYC | 1 cut(s) | 1830 |
| BssT1I | CCWWGG | 2 cut(s) | 1367, 1860 |
| Bst1107I | GTATAC | 1 cut(s) | 1025 |
| Bst2UI | CCWGG | 3 cut(s) | 271, 474, 1815 |
| Bst4CI | ACNGT | 7 cut(s) | 13, 31, 291, 570, 736, 1405, 1804 |
| Bst6I | CTCTTC | 5 cut(s) | 318, 522, 1077, 1313, 1912 |
| BstACI | GRCGYC | 1 cut(s) | 1830 |
| BstAFI | CTTAAG | 1 cut(s) | 815 |
| BstBAI | YACGTR | 1 cut(s) | 1187 |
| BstC8I | GCNNGC | 4 cut(s) | 201, 985, 989, 1241 |
| BstDEI | CTNAG | 4 cut(s) | 231, 672, 1056, 1638 |
| BstF5I | GGATG | 3 cut(s) | 121, 1976, 2148 |
| BstFNI | CGCG | 1 cut(s) | 1119 |
| BstHHI | GCGC | 2 cut(s) | 1577, 1626 |
| BstKTI | GATC | 6 cut(s) | 721, 1093, 1270, 1383, 1645, 1875 |
| BstMAI | GTCTC | 2 cut(s) | 320, 1351 |
| BstMBI | GATC | 6 cut(s) | 718, 1090, 1267, 1380, 1642, 1872 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 200, 266, 485, 962, 1766, 1805 |
| BstNI | CCWGG | 3 cut(s) | 271, 474, 1815 |
| BstNSI | RCATGY | 3 cut(s) | 1243, 1334, 1529 |
| BstSCI | CCNGG | 4 cut(s) | 269, 431, 472, 1813 |
| BstUI | CGCG | 1 cut(s) | 1119 |
| BstV1I | GCAGC | 6 cut(s) | 17, 1509, 1589, 1730, 1756, 1783 |
| BstV2I | GAAGAC | 1 cut(s) | 159 |
| BstX2I | RGATCY | 2 cut(s) | 1642, 1872 |
| BstYI | RGATCY | 2 cut(s) | 1642, 1872 |
| BstZ17I | GTATAC | 1 cut(s) | 1025 |
| Bsu36I | CCTNAGG | 2 cut(s) | 1056, 1638 |
| BsuI | GTATCC | 1 cut(s) | 640 |
| BsuRI | GGCC | 2 cut(s) | 431, 2033 |
| BtgZI | GCGATG | 2 cut(s) | 1412, 1881 |
| BtrI | CACGTC | 1 cut(s) | 544 |
| BtsCI | GGATG | 3 cut(s) | 121, 1976, 2148 |
| BtsI | GCAGTG | 1 cut(s) | 1323 |
| BtsIMutI | CAGTG | 2 cut(s) | 1323, 1809 |
| BtuMI | TCGCGA | 1 cut(s) | 1119 |
| Cac8I | GCNNGC | 4 cut(s) | 201, 985, 989, 1241 |
| CfoI | GCGC | 2 cut(s) | 1577, 1626 |
| Cfr10I | RCCGGY | 1 cut(s) | 548 |
| Cfr13I | GGNCC | 4 cut(s) | 430, 826, 1294, 2031 |
| CseI | GACGC | 1 cut(s) | 337 |
| Csp6I | GTAC | 3 cut(s) | 960, 1182, 2108 |
| CspAI | ACCGGT | 1 cut(s) | 548 |
| CviQI | GTAC | 3 cut(s) | 960, 1182, 2108 |
| DdeI | CTNAG | 4 cut(s) | 231, 672, 1056, 1638 |
| DpnI | GATC | 6 cut(s) | 720, 1092, 1269, 1382, 1644, 1874 |
| DpnII | GATC | 6 cut(s) | 718, 1090, 1267, 1380, 1642, 1872 |
| DraI | TTTAAA | 2 cut(s) | 945, 2200 |
| DraIII | CACNNNGTG | 1 cut(s) | 1679 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 356 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 356 |
| Eam1104I | CTCTTC | 5 cut(s) | 318, 522, 1077, 1313, 1912 |
| EarI | CTCTTC | 5 cut(s) | 318, 522, 1077, 1313, 1912 |
| Eco130I | CCWWGG | 2 cut(s) | 1367, 1860 |
| Eco31I | GGTCTC | 1 cut(s) | 1351 |
| Eco32I | GATATC | 1 cut(s) | 2167 |
| Eco47I | GGWCC | 2 cut(s) | 826, 1294 |
| Eco57I | CTGAAG | 2 cut(s) | 123, 1902 |
| Eco81I | CCTNAGG | 2 cut(s) | 1056, 1638 |
| Eco88I | CYCGRG | 1 cut(s) | 343 |
| EcoRI | GAATTC | 1 cut(s) | 161 |
| EcoRII | CCWGG | 3 cut(s) | 269, 472, 1813 |
| EcoRV | GATATC | 1 cut(s) | 2167 |
| EcoT14I | CCWWGG | 2 cut(s) | 1367, 1860 |
| EcoT22I | ATGCAT | 2 cut(s) | 952, 1241 |
| ErhI | CCWWGG | 2 cut(s) | 1367, 1860 |
| Esp3I | CGTCTC | 1 cut(s) | 320 |
| FaqI | GGGAC | 2 cut(s) | 2, 839 |
| FauI | CCCGC | 2 cut(s) | 212, 412 |
| FblI | GTMKAC | 2 cut(s) | 600, 1024 |
| Fnu4HI | GCNGC | 6 cut(s) | 6, 1523, 1578, 1719, 1770, 1797 |
| FokI | GGATG | 3 cut(s) | 128, 1963, 2155 |
| Fsp4HI | GCNGC | 6 cut(s) | 6, 1523, 1578, 1719, 1770, 1797 |
| FspBI | CTAG | 6 cut(s) | 21, 638, 957, 984, 1145, 2055 |
| GlaI | GCGC | 2 cut(s) | 1576, 1625 |
| GluI | GCNGC | 6 cut(s) | 6, 1523, 1578, 1719, 1770, 1797 |
| GsuI | CTGGAG | 2 cut(s) | 303, 1158 |
| HaeIII | GGCC | 2 cut(s) | 431, 2033 |
| HapII | CCGG | 3 cut(s) | 433, 549, 930 |
| HgaI | GACGC | 1 cut(s) | 337 |
| HhaI | GCGC | 2 cut(s) | 1577, 1626 |
| Hin1I | GRCGYC | 1 cut(s) | 1830 |
| Hin6I | GCGC | 2 cut(s) | 1575, 1624 |
| HinP1I | GCGC | 2 cut(s) | 1575, 1624 |
| HincII | GTYRAC | 1 cut(s) | 340 |
| HindII | GTYRAC | 1 cut(s) | 340 |
| HindIII | AAGCTT | 2 cut(s) | 812, 904 |
| HinfI | GANTC | 7 cut(s) | 233, 341, 935, 1108, 1173, 1938, 2187 |
| HpaII | CCGG | 3 cut(s) | 433, 549, 930 |
| HphI | GGTGA | 2 cut(s) | 94, 1154 |
| Hpy166II | GTNNAC | 4 cut(s) | 340, 601, 1025, 1184 |
| Hpy188I | TCNGA | 8 cut(s) | 756, 995, 1077, 1104, 1663, 1872, 1960, 2192 |
| Hpy8I | GTNNAC | 4 cut(s) | 340, 601, 1025, 1184 |
| HpyAV | CCTTC | 7 cut(s) | 98, 445, 716, 732, 1169, 1530, 2168 |
| HpyCH4III | ACNGT | 7 cut(s) | 13, 31, 291, 570, 736, 1405, 1804 |
| HpyCH4IV | ACGT | 6 cut(s) | 409, 543, 758, 1186, 1830, 1951 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 200, 266, 485, 962, 1766, 1805 |
| HpyF3I | CTNAG | 4 cut(s) | 231, 672, 1056, 1638 |
| HpySE526I | ACGT | 6 cut(s) | 409, 543, 758, 1186, 1830, 1951 |
| Hsp92I | GRCGYC | 1 cut(s) | 1830 |
| HspAI | GCGC | 2 cut(s) | 1575, 1624 |
| Kzo9I | GATC | 6 cut(s) | 718, 1090, 1267, 1380, 1642, 1872 |
| LguI | GCTCTTC | 2 cut(s) | 522, 1912 |
| LmnI | GCTCC | 4 cut(s) | 454, 1212, 1339, 2076 |
| Lsp1109I | GCAGC | 6 cut(s) | 17, 1509, 1589, 1730, 1756, 1783 |
| LweI | GCATC | 5 cut(s) | 217, 256, 784, 1435, 2133 |
| MaeI | CTAG | 6 cut(s) | 21, 638, 957, 984, 1145, 2055 |
| MaeII | ACGT | 6 cut(s) | 409, 543, 758, 1186, 1830, 1951 |
| MaeIII | GTNAC | 6 cut(s) | 25, 347, 358, 544, 1831, 1891 |
| MalI | GATC | 6 cut(s) | 720, 1092, 1269, 1382, 1644, 1874 |
| MboI | GATC | 6 cut(s) | 718, 1090, 1267, 1380, 1642, 1872 |
| MfeI | CAATTG | 2 cut(s) | 363, 2124 |
| MflI | RGATCY | 2 cut(s) | 1642, 1872 |
| MlyI | GAGTC | 2 cut(s) | 242, 335 |
| MmeI | TCCRAC | 3 cut(s) | 376, 741, 861 |
| Mph1103I | ATGCAT | 2 cut(s) | 952, 1241 |
| MslI | CAYNNNNRTG | 1 cut(s) | 2137 |
| MspCI | CTTAAG | 1 cut(s) | 815 |
| MspI | CCGG | 3 cut(s) | 433, 549, 930 |
| MspR9I | CCNGG | 4 cut(s) | 271, 433, 474, 1815 |
| MunI | CAATTG | 2 cut(s) | 363, 2124 |
| MvaI | CCWGG | 3 cut(s) | 271, 474, 1815 |
| MvnI | CGCG | 1 cut(s) | 1119 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 200, 266, 485, 962, 1766, 1805 |
| NciI | CCSGG | 1 cut(s) | 433 |
| NdeII | GATC | 6 cut(s) | 718, 1090, 1267, 1380, 1642, 1872 |
| NheI | GCTAGC | 1 cut(s) | 983 |
| NlaIV | GGNNCC | 3 cut(s) | 827, 1373, 2032 |
| NmeAIII | GCCGAG | 1 cut(s) | 859 |
| NmuCI | GTSAC | 4 cut(s) | 347, 358, 544, 1831 |
| NruI | TCGCGA | 1 cut(s) | 1119 |
| NsiI | ATGCAT | 2 cut(s) | 952, 1241 |
| NspI | RCATGY | 3 cut(s) | 1243, 1334, 1529 |
| PaeI | GCATGC | 1 cut(s) | 1243 |
| PaeR7I | CTCGAG | 1 cut(s) | 343 |
| PciSI | GCTCTTC | 2 cut(s) | 522, 1912 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1675 |
| PfeI | GAWTC | 5 cut(s) | 935, 1108, 1173, 1938, 2187 |
| PflFI | GACNNNGTC | 1 cut(s) | 353 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1318 |
| PinAI | ACCGGT | 1 cut(s) | 548 |
| PkrI | GCNGC | 6 cut(s) | 7, 1524, 1579, 1720, 1771, 1798 |
| PleI | GAGTC | 2 cut(s) | 241, 335 |
| PpsI | GAGTC | 2 cut(s) | 241, 335 |
| Ppu21I | YACGTR | 1 cut(s) | 1187 |
| PshBI | ATTAAT | 1 cut(s) | 183 |
| PsiI | TTATAA | 1 cut(s) | 1017 |
| Psp1406I | AACGTT | 1 cut(s) | 409 |
| Psp6I | CCWGG | 3 cut(s) | 269, 472, 1813 |
| PspGI | CCWGG | 3 cut(s) | 269, 472, 1813 |
| PspN4I | GGNNCC | 3 cut(s) | 827, 1373, 2032 |
| PspPI | GGNCC | 4 cut(s) | 430, 826, 1294, 2031 |
| PspXI | VCTCGAGB | 1 cut(s) | 343 |
| PsuI | RGATCY | 2 cut(s) | 1642, 1872 |
| PsyI | GACNNNGTC | 1 cut(s) | 353 |
| RruI | TCGCGA | 1 cut(s) | 1119 |
| RsaI | GTAC | 3 cut(s) | 961, 1183, 2109 |
| RsaNI | GTAC | 3 cut(s) | 960, 1182, 2108 |
| RseI | CAYNNNNRTG | 1 cut(s) | 2137 |
| SapI | GCTCTTC | 2 cut(s) | 522, 1912 |
| SatI | GCNGC | 6 cut(s) | 6, 1523, 1578, 1719, 1770, 1797 |
| Sau3AI | GATC | 6 cut(s) | 718, 1090, 1267, 1380, 1642, 1872 |
| Sau96I | GGNCC | 4 cut(s) | 430, 826, 1294, 2031 |
| SchI | GAGTC | 2 cut(s) | 242, 335 |
| ScrFI | CCNGG | 4 cut(s) | 271, 433, 474, 1815 |
| SfaNI | GCATC | 5 cut(s) | 217, 256, 784, 1435, 2133 |
| Sfr274I | CTCGAG | 1 cut(s) | 343 |
| SinI | GGWCC | 2 cut(s) | 826, 1294 |
| SlaI | CTCGAG | 1 cut(s) | 343 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 2137 |
| SmlI | CTYRAG | 3 cut(s) | 343, 815, 918 |
| SmoI | CTYRAG | 3 cut(s) | 343, 815, 918 |
| SpeI | ACTAGT | 1 cut(s) | 1144 |
| SphI | GCATGC | 1 cut(s) | 1243 |
| SsiI | CCGC | 2 cut(s) | 219, 419 |
| SspI | AATATT | 3 cut(s) | 37, 277, 999 |
| SspMI | CTAG | 6 cut(s) | 21, 638, 957, 984, 1145, 2055 |
| StyD4I | CCNGG | 4 cut(s) | 269, 431, 472, 1813 |
| StyI | CCWWGG | 2 cut(s) | 1367, 1860 |
| TaaI | ACNGT | 7 cut(s) | 13, 31, 291, 570, 736, 1405, 1804 |
| TaiI | ACGT | 6 cut(s) | 412, 546, 761, 1189, 1833, 1954 |
| TaqI | TCGA | 6 cut(s) | 123, 344, 717, 1224, 1570, 1620 |
| TaqII | GACCGA | 2 cut(s) | 677, 843 |
| TfiI | GAWTC | 5 cut(s) | 935, 1108, 1173, 1938, 2187 |
| TscAI | CASTG | 2 cut(s) | 1323, 1809 |
| TseFI | GTSAC | 4 cut(s) | 347, 358, 544, 1831 |
| TseI | GCWGC | 6 cut(s) | 5, 1522, 1577, 1718, 1769, 1796 |
| Tsp45I | GTSAC | 4 cut(s) | 347, 358, 544, 1831 |
| TspRI | CASTG | 2 cut(s) | 1323, 1809 |
| Tth111I | GACNNNGTC | 1 cut(s) | 353 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1318 |
| Vha464I | CTTAAG | 1 cut(s) | 815 |
| VpaK11BI | GGWCC | 2 cut(s) | 826, 1294 |
| VspI | ATTAAT | 1 cut(s) | 183 |
| XapI | RAATTY | 8 cut(s) | 161, 306, 491, 1227, 1460, 1657, 1685, 1705 |
| XceI | RCATGY | 3 cut(s) | 1243, 1334, 1529 |
| XhoI | CTCGAG | 1 cut(s) | 343 |
| XmiI | GTMKAC | 2 cut(s) | 600, 1024 |
| XspI | CTAG | 6 cut(s) | 21, 638, 957, 984, 1145, 2055 |
| ZraI | GACGTC | 1 cut(s) | 1831 |
| Zsp2I | ATGCAT | 2 cut(s) | 952, 1241 |