Domain of unknown function
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 242, 924 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1228 |
| AccI | GTMKAC | 1 cut(s) | 931 |
| AccII | CGCG | 2 cut(s) | 1026, 1093 |
| AccIII | TCCGGA | 1 cut(s) | 1076 |
| AciI | CCGC | 3 cut(s) | 219, 419, 663 |
| AclWI | GGATC | 2 cut(s) | 80, 1560 |
| AcsI | RAATTY | 9 cut(s) | 161, 276, 306, 491, 1134, 1370, 1567, 1595, 1615 |
| AcuI | CTGAAG | 1 cut(s) | 123 |
| AdeI | CACNNNGTG | 1 cut(s) | 1589 |
| AfaI | GTAC | 2 cut(s) | 868, 1090 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 438, 439, 538, 1228 |
| AflII | CTTAAG | 1 cut(s) | 722 |
| AflIII | ACRYGT | 1 cut(s) | 1091 |
| AgeI | ACCGGT | 1 cut(s) | 548 |
| AgsI | TTSAA | 6 cut(s) | 295, 305, 346, 716, 1322, 1522 |
| AhlI | ACTAGT | 1 cut(s) | 1051 |
| AjnI | CCWGG | 3 cut(s) | 188, 269, 1723 |
| AleI | CACNNNNGTG | 1 cut(s) | 837 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 218, 250 |
| AluBI | AGCT | 8 cut(s) | 44, 203, 515, 721, 745, 898, 1490, 1679 |
| AluI | AGCT | 8 cut(s) | 44, 203, 515, 721, 745, 898, 1490, 1679 |
| Alw26I | GTCTC | 2 cut(s) | 320, 698 |
| AlwI | GGATC | 2 cut(s) | 80, 1560 |
| Ama87I | CYCGRG | 1 cut(s) | 474 |
| Aor13HI | TCCGGA | 1 cut(s) | 1076 |
| AoxI | GGCC | 1 cut(s) | 192 |
| ApeKI | GCWGC | 5 cut(s) | 5, 1432, 1487, 1679, 1706 |
| ApoI | RAATTY | 9 cut(s) | 161, 276, 306, 491, 1134, 1370, 1567, 1595, 1615 |
| AseI | ATTAAT | 1 cut(s) | 183 |
| AsiGI | ACCGGT | 1 cut(s) | 548 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 290 |
| AspLEI | GCGC | 1 cut(s) | 1536 |
| AspS9I | GGNCC | 3 cut(s) | 192, 733, 1204 |
| AsuHPI | GGTGA | 2 cut(s) | 295, 851 |
| AsuII | TTCGAA | 1 cut(s) | 496 |
| AsuNHI | GCTAGC | 1 cut(s) | 890 |
| AvaI | CYCGRG | 1 cut(s) | 474 |
| AvaII | GGWCC | 2 cut(s) | 733, 1204 |
| AxyI | CCTNAGG | 2 cut(s) | 963, 1548 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 639, 672 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 446, 478 |
| BbsI | GAAGAC | 2 cut(s) | 159, 381 |
| BbvI | GCAGC | 5 cut(s) | 17, 1419, 1499, 1666, 1693 |
| BccI | CCATC | 3 cut(s) | 602, 909, 1136 |
| BceAI | ACGGC | 2 cut(s) | 273, 885 |
| BciT130I | CCWGG | 3 cut(s) | 190, 271, 1725 |
| BciVI | GTATCC | 1 cut(s) | 640 |
| BcoDI | GTCTC | 2 cut(s) | 320, 698 |
| BcuI | ACTAGT | 1 cut(s) | 1051 |
| BfaI | CTAG | 8 cut(s) | 21, 599, 638, 672, 864, 891, 1052, 1286 |
| BfmI | CTRYAG | 2 cut(s) | 972, 1139 |
| BfrI | CTTAAG | 1 cut(s) | 722 |
| BfuI | GTATCC | 1 cut(s) | 640 |
| BisI | GCNGC | 5 cut(s) | 6, 1433, 1488, 1680, 1707 |
| BlsI | GCNGC | 5 cut(s) | 7, 1434, 1489, 1681, 1708 |
| Bme1390I | CCNGG | 3 cut(s) | 190, 271, 1725 |
| Bme18I | GGWCC | 2 cut(s) | 733, 1204 |
| BmeT110I | CYCGRG | 1 cut(s) | 474 |
| BmgT120I | GGNCC | 3 cut(s) | 192, 733, 1204 |
| BmiI | GGNNCC | 2 cut(s) | 734, 1283 |
| BmrFI | CCNGG | 3 cut(s) | 190, 271, 1725 |
| BmsI | GCATC | 4 cut(s) | 217, 256, 691, 1345 |
| BmtI | GCTAGC | 1 cut(s) | 894 |
| BpiI | GAAGAC | 2 cut(s) | 159, 381 |
| BpmI | CTGGAG | 1 cut(s) | 1065 |
| Bpu14I | TTCGAA | 1 cut(s) | 496 |
| BpuEI | CTTGAG | 1 cut(s) | 810 |
| BsaJI | CCNNGG | 4 cut(s) | 189, 270, 473, 1277 |
| BsaWI | WCCGGW | 2 cut(s) | 548, 1076 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1038, 1068 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 438, 439, 538, 1228 |
| Bse118I | RCCGGY | 1 cut(s) | 548 |
| Bse1I | ACTGG | 2 cut(s) | 74, 532 |
| Bse21I | CCTNAGG | 2 cut(s) | 963, 1548 |
| BseAI | TCCGGA | 1 cut(s) | 1076 |
| BseBI | CCWGG | 3 cut(s) | 190, 271, 1725 |
| BseDI | CCNNGG | 4 cut(s) | 189, 270, 473, 1277 |
| BseGI | GGATG | 2 cut(s) | 232, 658 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 438, 439, 538, 1228 |
| BseMII | CTCAG | 2 cut(s) | 222, 954 |
| BseNI | ACTGG | 2 cut(s) | 74, 532 |
| BseXI | GCAGC | 5 cut(s) | 17, 1419, 1499, 1666, 1693 |
| BsgI | GTGCAG | 2 cut(s) | 1052, 1506 |
| Bsh1236I | CGCG | 2 cut(s) | 1026, 1093 |
| BshFI | GGCC | 1 cut(s) | 194 |
| BshTI | ACCGGT | 1 cut(s) | 548 |
| BsiHKCI | CYCGRG | 1 cut(s) | 474 |
| BsiSI | CCGG | 2 cut(s) | 549, 1077 |
| BsiWI | CGTACG | 1 cut(s) | 1088 |
| BslFI | GGGAC | 3 cut(s) | 2, 554, 746 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 438, 439, 538, 1228 |
| BsmAI | GTCTC | 2 cut(s) | 320, 698 |
| BsmBI | CGTCTC | 1 cut(s) | 320 |
| BsmFI | GGGAC | 3 cut(s) | 2, 554, 746 |
| BsnI | GGCC | 1 cut(s) | 194 |
| BsoBI | CYCGRG | 1 cut(s) | 474 |
| Bsp119I | TTCGAA | 1 cut(s) | 496 |
| Bsp13I | TCCGGA | 1 cut(s) | 1076 |
| Bsp143I | GATC | 5 cut(s) | 72, 997, 1174, 1290, 1552 |
| Bsp68I | TCGCGA | 1 cut(s) | 1026 |
| BspACI | CCGC | 3 cut(s) | 219, 419, 663 |
| BspANI | GGCC | 1 cut(s) | 194 |
| BspCNI | CTCAG | 2 cut(s) | 223, 955 |
| BspEI | TCCGGA | 1 cut(s) | 1076 |
| BspFNI | CGCG | 2 cut(s) | 1026, 1093 |
| BspLI | GGNNCC | 2 cut(s) | 734, 1283 |
| BspOI | GCTAGC | 1 cut(s) | 894 |
| BspPI | GGATC | 2 cut(s) | 80, 1560 |
| BspQI | GCTCTTC | 1 cut(s) | 522 |
| BspT104I | TTCGAA | 1 cut(s) | 496 |
| BspTI | CTTAAG | 1 cut(s) | 722 |
| BsrFI | RCCGGY | 1 cut(s) | 548 |
| BsrI | ACTGG | 2 cut(s) | 74, 532 |
| BssAI | RCCGGY | 1 cut(s) | 548 |
| BssECI | CCNNGG | 4 cut(s) | 189, 270, 473, 1277 |
| BssMI | GATC | 5 cut(s) | 72, 997, 1174, 1290, 1552 |
| BssNAI | GTATAC | 1 cut(s) | 932 |
| BssT1I | CCWWGG | 1 cut(s) | 1277 |
| Bst1107I | GTATAC | 1 cut(s) | 932 |
| Bst2UI | CCWGG | 3 cut(s) | 190, 271, 1725 |
| Bst4CI | ACNGT | 7 cut(s) | 13, 291, 570, 605, 1143, 1315, 1714 |
| Bst6I | CTCTTC | 5 cut(s) | 318, 522, 984, 1223, 1755 |
| BstAFI | CTTAAG | 1 cut(s) | 722 |
| BstBI | TTCGAA | 1 cut(s) | 496 |
| BstC8I | GCNNGC | 5 cut(s) | 99, 201, 892, 896, 1148 |
| BstDEI | CTNAG | 4 cut(s) | 231, 589, 963, 1548 |
| BstF5I | GGATG | 2 cut(s) | 232, 658 |
| BstFNI | CGCG | 2 cut(s) | 1026, 1093 |
| BstHHI | GCGC | 1 cut(s) | 1536 |
| BstKTI | GATC | 5 cut(s) | 75, 1000, 1177, 1293, 1555 |
| BstMAI | GTCTC | 2 cut(s) | 320, 698 |
| BstMBI | GATC | 5 cut(s) | 72, 997, 1174, 1290, 1552 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 200, 266, 869, 1676 |
| BstNI | CCWGG | 3 cut(s) | 190, 271, 1725 |
| BstNSI | RCATGY | 4 cut(s) | 1150, 1244, 1432, 1439 |
| BstSCI | CCNGG | 3 cut(s) | 188, 269, 1723 |
| BstSFI | CTRYAG | 2 cut(s) | 972, 1139 |
| BstUI | CGCG | 2 cut(s) | 1026, 1093 |
| BstV1I | GCAGC | 5 cut(s) | 17, 1419, 1499, 1666, 1693 |
| BstV2I | GAAGAC | 2 cut(s) | 159, 381 |
| BstX2I | RGATCY | 2 cut(s) | 72, 1552 |
| BstYI | RGATCY | 2 cut(s) | 72, 1552 |
| BstZ17I | GTATAC | 1 cut(s) | 932 |
| Bsu36I | CCTNAGG | 2 cut(s) | 963, 1548 |
| BsuI | GTATCC | 1 cut(s) | 640 |
| BsuRI | GGCC | 1 cut(s) | 194 |
| BtgZI | GCGATG | 1 cut(s) | 1322 |
| BtsCI | GGATG | 2 cut(s) | 232, 658 |
| BtsI | GCAGTG | 1 cut(s) | 1233 |
| BtsIMutI | CAGTG | 3 cut(s) | 1148, 1233, 1719 |
| BtuMI | TCGCGA | 1 cut(s) | 1026 |
| Cac8I | GCNNGC | 5 cut(s) | 99, 201, 892, 896, 1148 |
| CfoI | GCGC | 1 cut(s) | 1536 |
| Cfr10I | RCCGGY | 1 cut(s) | 548 |
| Cfr13I | GGNCC | 3 cut(s) | 192, 733, 1204 |
| CseI | GACGC | 1 cut(s) | 337 |
| Csp6I | GTAC | 2 cut(s) | 867, 1089 |
| CspAI | ACCGGT | 1 cut(s) | 548 |
| CviQI | GTAC | 2 cut(s) | 867, 1089 |
| DdeI | CTNAG | 4 cut(s) | 231, 589, 963, 1548 |
| DpnI | GATC | 5 cut(s) | 74, 999, 1176, 1292, 1554 |
| DpnII | GATC | 5 cut(s) | 72, 997, 1174, 1290, 1552 |
| DraI | TTTAAA | 2 cut(s) | 852, 1755 |
| DraIII | CACNNNGTG | 1 cut(s) | 1589 |
| Eam1104I | CTCTTC | 5 cut(s) | 318, 522, 984, 1223, 1755 |
| EarI | CTCTTC | 5 cut(s) | 318, 522, 984, 1223, 1755 |
| Eco130I | CCWWGG | 1 cut(s) | 1277 |
| Eco47I | GGWCC | 2 cut(s) | 733, 1204 |
| Eco57I | CTGAAG | 1 cut(s) | 123 |
| Eco81I | CCTNAGG | 2 cut(s) | 963, 1548 |
| Eco88I | CYCGRG | 1 cut(s) | 474 |
| EcoO109I | RGGNCCY | 1 cut(s) | 192 |
| EcoRI | GAATTC | 1 cut(s) | 161 |
| EcoRII | CCWGG | 3 cut(s) | 188, 269, 1723 |
| EcoT14I | CCWWGG | 1 cut(s) | 1277 |
| EcoT22I | ATGCAT | 1 cut(s) | 859 |
| ErhI | CCWWGG | 1 cut(s) | 1277 |
| Esp3I | CGTCTC | 1 cut(s) | 320 |
| FaqI | GGGAC | 3 cut(s) | 2, 554, 746 |
| FauI | CCCGC | 1 cut(s) | 212 |
| FauNDI | CATATG | 1 cut(s) | 1573 |
| FblI | GTMKAC | 1 cut(s) | 931 |
| Fnu4HI | GCNGC | 5 cut(s) | 6, 1433, 1488, 1680, 1707 |
| FokI | GGATG | 2 cut(s) | 239, 665 |
| Fsp4HI | GCNGC | 5 cut(s) | 6, 1433, 1488, 1680, 1707 |
| FspBI | CTAG | 8 cut(s) | 21, 599, 638, 672, 864, 891, 1052, 1286 |
| GlaI | GCGC | 1 cut(s) | 1535 |
| GluI | GCNGC | 5 cut(s) | 6, 1433, 1488, 1680, 1707 |
| GsuI | CTGGAG | 1 cut(s) | 1065 |
| HaeIII | GGCC | 1 cut(s) | 194 |
| HapII | CCGG | 2 cut(s) | 549, 1077 |
| HgaI | GACGC | 1 cut(s) | 337 |
| HhaI | GCGC | 1 cut(s) | 1536 |
| Hin6I | GCGC | 1 cut(s) | 1534 |
| HinP1I | GCGC | 1 cut(s) | 1534 |
| HincII | GTYRAC | 1 cut(s) | 340 |
| HindII | GTYRAC | 1 cut(s) | 340 |
| HindIII | AAGCTT | 1 cut(s) | 719 |
| HinfI | GANTC | 4 cut(s) | 354, 842, 1015, 1080 |
| HpaII | CCGG | 2 cut(s) | 549, 1077 |
| HphI | GGTGA | 2 cut(s) | 295, 851 |
| Hpy166II | GTNNAC | 3 cut(s) | 340, 932, 1606 |
| Hpy188I | TCNGA | 3 cut(s) | 902, 984, 1011 |
| Hpy8I | GTNNAC | 3 cut(s) | 340, 932, 1606 |
| Hpy99I | CGWCG | 1 cut(s) | 259 |
| HpyAV | CCTTC | 4 cut(s) | 119, 383, 1440, 1519 |
| HpyCH4III | ACNGT | 7 cut(s) | 13, 291, 570, 605, 1143, 1315, 1714 |
| HpyCH4IV | ACGT | 2 cut(s) | 384, 409 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 200, 266, 869, 1676 |
| HpyF3I | CTNAG | 4 cut(s) | 231, 589, 963, 1548 |
| HpySE526I | ACGT | 2 cut(s) | 384, 409 |
| HspAI | GCGC | 1 cut(s) | 1534 |
| Kpn2I | TCCGGA | 1 cut(s) | 1076 |
| Kzo9I | GATC | 5 cut(s) | 72, 997, 1174, 1290, 1552 |
| LguI | GCTCTTC | 1 cut(s) | 522 |
| LmnI | GCTCC | 3 cut(s) | 1119, 1249, 1531 |
| Lsp1109I | GCAGC | 5 cut(s) | 17, 1419, 1499, 1666, 1693 |
| LweI | GCATC | 4 cut(s) | 217, 256, 691, 1345 |
| MaeI | CTAG | 8 cut(s) | 21, 599, 638, 672, 864, 891, 1052, 1286 |
| MaeII | ACGT | 2 cut(s) | 384, 409 |
| MaeIII | GTNAC | 2 cut(s) | 1037, 1714 |
| MalI | GATC | 5 cut(s) | 74, 999, 1176, 1292, 1554 |
| MboI | GATC | 5 cut(s) | 72, 997, 1174, 1290, 1552 |
| MfeI | CAATTG | 1 cut(s) | 363 |
| MflI | RGATCY | 2 cut(s) | 72, 1552 |
| MluI | ACGCGT | 1 cut(s) | 1091 |
| MlyI | GAGTC | 1 cut(s) | 363 |
| MmeI | TCCRAC | 2 cut(s) | 376, 768 |
| Mph1103I | ATGCAT | 1 cut(s) | 859 |
| MroI | TCCGGA | 1 cut(s) | 1076 |
| MroXI | GAANNNNTTC | 1 cut(s) | 290 |
| MslI | CAYNNNNRTG | 2 cut(s) | 777, 837 |
| MspCI | CTTAAG | 1 cut(s) | 722 |
| MspI | CCGG | 2 cut(s) | 549, 1077 |
| MspR9I | CCNGG | 3 cut(s) | 190, 271, 1725 |
| MunI | CAATTG | 1 cut(s) | 363 |
| MvaI | CCWGG | 3 cut(s) | 190, 271, 1725 |
| MvnI | CGCG | 2 cut(s) | 1026, 1093 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 200, 266, 869, 1676 |
| NdeI | CATATG | 1 cut(s) | 1573 |
| NdeII | GATC | 5 cut(s) | 72, 997, 1174, 1290, 1552 |
| NheI | GCTAGC | 1 cut(s) | 890 |
| NlaIV | GGNNCC | 2 cut(s) | 734, 1283 |
| NmeAIII | GCCGAG | 1 cut(s) | 766 |
| NmuCI | GTSAC | 1 cut(s) | 1714 |
| NruI | TCGCGA | 1 cut(s) | 1026 |
| NsiI | ATGCAT | 1 cut(s) | 859 |
| NspI | RCATGY | 4 cut(s) | 1150, 1244, 1432, 1439 |
| NspV | TTCGAA | 1 cut(s) | 496 |
| OliI | CACNNNNGTG | 1 cut(s) | 837 |
| PaeI | GCATGC | 1 cut(s) | 1150 |
| PciSI | GCTCTTC | 1 cut(s) | 522 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1585 |
| PdmI | GAANNNNTTC | 1 cut(s) | 290 |
| PfeI | GAWTC | 3 cut(s) | 842, 1015, 1080 |
| Pfl23II | CGTACG | 1 cut(s) | 1088 |
| PflFI | GACNNNGTC | 2 cut(s) | 353, 1397 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1228 |
| PinAI | ACCGGT | 1 cut(s) | 548 |
| PkrI | GCNGC | 5 cut(s) | 7, 1434, 1489, 1681, 1708 |
| PleI | GAGTC | 1 cut(s) | 362 |
| PpsI | GAGTC | 1 cut(s) | 362 |
| PshBI | ATTAAT | 1 cut(s) | 183 |
| PsiI | TTATAA | 2 cut(s) | 242, 924 |
| Psp6I | CCWGG | 3 cut(s) | 188, 269, 1723 |
| PspGI | CCWGG | 3 cut(s) | 188, 269, 1723 |
| PspLI | CGTACG | 1 cut(s) | 1088 |
| PspN4I | GGNNCC | 2 cut(s) | 734, 1283 |
| PspPI | GGNCC | 3 cut(s) | 192, 733, 1204 |
| PsuI | RGATCY | 2 cut(s) | 72, 1552 |
| PsyI | GACNNNGTC | 2 cut(s) | 353, 1397 |
| RruI | TCGCGA | 1 cut(s) | 1026 |
| RsaI | GTAC | 2 cut(s) | 868, 1090 |
| RsaNI | GTAC | 2 cut(s) | 867, 1089 |
| RseI | CAYNNNNRTG | 2 cut(s) | 777, 837 |
| SapI | GCTCTTC | 1 cut(s) | 522 |
| SatI | GCNGC | 5 cut(s) | 6, 1433, 1488, 1680, 1707 |
| Sau3AI | GATC | 5 cut(s) | 72, 997, 1174, 1290, 1552 |
| Sau96I | GGNCC | 3 cut(s) | 192, 733, 1204 |
| SchI | GAGTC | 1 cut(s) | 363 |
| ScrFI | CCNGG | 3 cut(s) | 190, 271, 1725 |
| SfaNI | GCATC | 4 cut(s) | 217, 256, 691, 1345 |
| SfcI | CTRYAG | 2 cut(s) | 972, 1139 |
| SfuI | TTCGAA | 1 cut(s) | 496 |
| SinI | GGWCC | 2 cut(s) | 733, 1204 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 777, 837 |
| SmlI | CTYRAG | 2 cut(s) | 722, 825 |
| SmoI | CTYRAG | 2 cut(s) | 722, 825 |
| SpeI | ACTAGT | 1 cut(s) | 1051 |
| SphI | GCATGC | 1 cut(s) | 1150 |
| SsiI | CCGC | 3 cut(s) | 219, 419, 663 |
| SspI | AATATT | 1 cut(s) | 1186 |
| SspMI | CTAG | 8 cut(s) | 21, 599, 638, 672, 864, 891, 1052, 1286 |
| StyD4I | CCNGG | 3 cut(s) | 188, 269, 1723 |
| StyI | CCWWGG | 1 cut(s) | 1277 |
| TaaI | ACNGT | 7 cut(s) | 13, 291, 570, 605, 1143, 1315, 1714 |
| TaiI | ACGT | 2 cut(s) | 387, 412 |
| TaqI | TCGA | 4 cut(s) | 123, 496, 1131, 1480 |
| TaqII | GACCGA | 1 cut(s) | 750 |
| TfiI | GAWTC | 3 cut(s) | 842, 1015, 1080 |
| TscAI | CASTG | 3 cut(s) | 1148, 1233, 1719 |
| TseFI | GTSAC | 1 cut(s) | 1714 |
| TseI | GCWGC | 5 cut(s) | 5, 1432, 1487, 1679, 1706 |
| Tsp45I | GTSAC | 1 cut(s) | 1714 |
| TspGWI | ACGGA | 1 cut(s) | 665 |
| TspRI | CASTG | 3 cut(s) | 1148, 1233, 1719 |
| Tth111I | GACNNNGTC | 2 cut(s) | 353, 1397 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1228 |
| Vha464I | CTTAAG | 1 cut(s) | 722 |
| VpaK11BI | GGWCC | 2 cut(s) | 733, 1204 |
| VspI | ATTAAT | 1 cut(s) | 183 |
| XapI | RAATTY | 9 cut(s) | 161, 276, 306, 491, 1134, 1370, 1567, 1595, 1615 |
| XceI | RCATGY | 4 cut(s) | 1150, 1244, 1432, 1439 |
| XmiI | GTMKAC | 1 cut(s) | 931 |
| XmnI | GAANNNNTTC | 1 cut(s) | 290 |
| XspI | CTAG | 8 cut(s) | 21, 599, 638, 672, 864, 891, 1052, 1286 |
| Zsp2I | ATGCAT | 1 cut(s) | 859 |