Domain of unknown function
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 898 |
| Acc36I | ACCTGC | 1 cut(s) | 1118 |
| AccB1I | GGYRCC | 1 cut(s) | 7 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 424 |
| AccI | GTMKAC | 1 cut(s) | 1063 |
| AciI | CCGC | 3 cut(s) | 5, 447, 754 |
| AclI | AACGTT | 1 cut(s) | 696 |
| AclWI | GGATC | 2 cut(s) | 408, 1058 |
| AcoI | YGGCCR | 3 cut(s) | 255, 948, 993 |
| AcsI | RAATTY | 4 cut(s) | 287, 409, 492, 905 |
| AcuI | CTGAAG | 3 cut(s) | 57, 384, 408 |
| AcvI | CACGTG | 1 cut(s) | 544 |
| AfaI | GTAC | 1 cut(s) | 50 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 253, 424, 503, 618, 1120 |
| AgsI | TTSAA | 5 cut(s) | 122, 691, 728, 932, 1034 |
| AjnI | CCWGG | 5 cut(s) | 266, 298, 349, 1114, 1145 |
| AluBI | AGCT | 5 cut(s) | 191, 851, 888, 986, 1139 |
| AluI | AGCT | 5 cut(s) | 191, 851, 888, 986, 1139 |
| Alw21I | GWGCWC | 1 cut(s) | 635 |
| Alw26I | GTCTC | 2 cut(s) | 600, 1159 |
| Alw44I | GTGCAC | 1 cut(s) | 631 |
| AlwI | GGATC | 2 cut(s) | 408, 1058 |
| Ama87I | CYCGRG | 1 cut(s) | 317 |
| AoxI | GGCC | 7 cut(s) | 244, 255, 486, 784, 948, 993, 1148 |
| ApaLI | GTGCAC | 1 cut(s) | 631 |
| ApoI | RAATTY | 4 cut(s) | 287, 409, 492, 905 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 413 |
| AspLEI | GCGC | 3 cut(s) | 797, 872, 899 |
| AspS9I | GGNCC | 2 cut(s) | 486, 1149 |
| AsuHPI | GGTGA | 1 cut(s) | 365 |
| AvaI | CYCGRG | 1 cut(s) | 317 |
| BaeGI | GKGCMC | 1 cut(s) | 635 |
| BalI | TGGCCA | 1 cut(s) | 950 |
| BanI | GGYRCC | 1 cut(s) | 7 |
| BbrPI | CACGTG | 1 cut(s) | 544 |
| BbsI | GAAGAC | 1 cut(s) | 372 |
| Bbv12I | GWGCWC | 1 cut(s) | 635 |
| BbvCI | CCTCAGC | 1 cut(s) | 248 |
| BccI | CCATC | 2 cut(s) | 426, 505 |
| BceAI | ACGGC | 1 cut(s) | 270 |
| BciT130I | CCWGG | 5 cut(s) | 268, 300, 351, 1116, 1147 |
| BclI | TGATCA | 2 cut(s) | 538, 625 |
| BcoDI | GTCTC | 2 cut(s) | 600, 1159 |
| BfaI | CTAG | 1 cut(s) | 983 |
| BfmI | CTRYAG | 1 cut(s) | 1140 |
| BfuAI | ACCTGC | 1 cut(s) | 1118 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 272 |
| BisI | GCNGC | 2 cut(s) | 6, 447 |
| BlpI | GCTNAGC | 1 cut(s) | 345 |
| BlsI | GCNGC | 2 cut(s) | 7, 448 |
| BmcAI | AGTACT | 1 cut(s) | 50 |
| Bme1390I | CCNGG | 5 cut(s) | 268, 300, 351, 1116, 1147 |
| BmeT110I | CYCGRG | 1 cut(s) | 317 |
| BmgT120I | GGNCC | 2 cut(s) | 486, 1149 |
| BmiI | GGNNCC | 1 cut(s) | 9 |
| BmrFI | CCNGG | 5 cut(s) | 268, 300, 351, 1116, 1147 |
| BmsI | GCATC | 5 cut(s) | 214, 246, 331, 433, 747 |
| BpiI | GAAGAC | 1 cut(s) | 372 |
| BpmI | CTGGAG | 1 cut(s) | 321 |
| Bpu10I | CCTNAGC | 1 cut(s) | 248 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 345 |
| BpuEI | CTTGAG | 2 cut(s) | 29, 1183 |
| Bsa29I | ATCGAT | 2 cut(s) | 403, 1089 |
| BsaAI | YACGTR | 1 cut(s) | 544 |
| BsaJI | CCNNGG | 4 cut(s) | 865, 967, 1114, 1115 |
| BsaWI | WCCGGW | 1 cut(s) | 23 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 253, 424, 503, 618, 1120 |
| Bse118I | RCCGGY | 1 cut(s) | 995 |
| Bse1I | ACTGG | 3 cut(s) | 11, 329, 779 |
| Bse3DI | GCAATG | 1 cut(s) | 456 |
| BseBI | CCWGG | 5 cut(s) | 268, 300, 351, 1116, 1147 |
| BseCI | ATCGAT | 2 cut(s) | 403, 1089 |
| BseDI | CCNNGG | 4 cut(s) | 865, 967, 1114, 1115 |
| BseGI | GGATG | 5 cut(s) | 118, 226, 448, 702, 762 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 253, 424, 503, 618, 1120 |
| BseMI | GCAATG | 1 cut(s) | 456 |
| BseMII | CTCAG | 3 cut(s) | 262, 336, 779 |
| BseNI | ACTGG | 3 cut(s) | 11, 329, 779 |
| BseRI | GAGGAG | 1 cut(s) | 969 |
| BseSI | GKGCMC | 1 cut(s) | 635 |
| BsgI | GTGCAG | 1 cut(s) | 593 |
| Bsh1285I | CGRYCG | 2 cut(s) | 24, 407 |
| BshFI | GGCC | 7 cut(s) | 246, 257, 488, 786, 950, 995, 1150 |
| BshNI | GGYRCC | 1 cut(s) | 7 |
| BshVI | ATCGAT | 2 cut(s) | 403, 1089 |
| BsiEI | CGRYCG | 2 cut(s) | 24, 407 |
| BsiHKAI | GWGCWC | 1 cut(s) | 635 |
| BsiHKCI | CYCGRG | 1 cut(s) | 317 |
| BsiSI | CCGG | 2 cut(s) | 24, 996 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 253, 424, 503, 618, 1120 |
| BsmAI | GTCTC | 2 cut(s) | 600, 1159 |
| BsmBI | CGTCTC | 1 cut(s) | 1159 |
| BsnI | GGCC | 7 cut(s) | 246, 257, 488, 786, 950, 995, 1150 |
| BsoBI | CYCGRG | 1 cut(s) | 317 |
| Bsp1286I | GDGCHC | 1 cut(s) | 635 |
| Bsp143I | GATC | 5 cut(s) | 400, 404, 538, 625, 1050 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 345 |
| Bsp19I | CCATGG | 1 cut(s) | 865 |
| BspACI | CCGC | 3 cut(s) | 5, 447, 754 |
| BspANI | GGCC | 7 cut(s) | 246, 257, 488, 786, 950, 995, 1150 |
| BspCNI | CTCAG | 3 cut(s) | 261, 337, 778 |
| BspDI | ATCGAT | 2 cut(s) | 403, 1089 |
| BspLI | GGNNCC | 1 cut(s) | 9 |
| BspMI | ACCTGC | 1 cut(s) | 1118 |
| BspPI | GGATC | 2 cut(s) | 408, 1058 |
| BspT107I | GGYRCC | 1 cut(s) | 7 |
| BsrDI | GCAATG | 1 cut(s) | 456 |
| BsrFI | RCCGGY | 1 cut(s) | 995 |
| BsrI | ACTGG | 3 cut(s) | 11, 329, 779 |
| BssAI | RCCGGY | 1 cut(s) | 995 |
| BssECI | CCNNGG | 4 cut(s) | 865, 967, 1114, 1115 |
| BssMI | GATC | 5 cut(s) | 400, 404, 538, 625, 1050 |
| BssNAI | GTATAC | 1 cut(s) | 1064 |
| BssT1I | CCWWGG | 1 cut(s) | 865 |
| Bst1107I | GTATAC | 1 cut(s) | 1064 |
| Bst2UI | CCWGG | 5 cut(s) | 268, 300, 351, 1116, 1147 |
| Bst4CI | ACNGT | 3 cut(s) | 860, 955, 968 |
| BstBAI | YACGTR | 1 cut(s) | 544 |
| BstC8I | GCNNGC | 1 cut(s) | 273 |
| BstDEI | CTNAG | 4 cut(s) | 162, 248, 345, 765 |
| BstDSI | CCRYGG | 2 cut(s) | 865, 967 |
| BstF5I | GGATG | 5 cut(s) | 118, 226, 448, 702, 762 |
| BstHHI | GCGC | 3 cut(s) | 797, 872, 899 |
| BstKTI | GATC | 5 cut(s) | 403, 407, 541, 628, 1053 |
| BstMAI | GTCTC | 2 cut(s) | 600, 1159 |
| BstMBI | GATC | 5 cut(s) | 400, 404, 538, 625, 1050 |
| BstMCI | CGRYCG | 2 cut(s) | 24, 407 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 243, 263, 272, 792, 801, 894, 992 |
| BstNI | CCWGG | 5 cut(s) | 268, 300, 351, 1116, 1147 |
| BstNSI | RCATGY | 1 cut(s) | 200 |
| BstSCI | CCNGG | 5 cut(s) | 266, 298, 349, 1114, 1145 |
| BstSFI | CTRYAG | 1 cut(s) | 1140 |
| BstSLI | GKGCMC | 1 cut(s) | 635 |
| BstV2I | GAAGAC | 1 cut(s) | 372 |
| BstX2I | RGATCY | 1 cut(s) | 1050 |
| BstYI | RGATCY | 1 cut(s) | 1050 |
| BstZ17I | GTATAC | 1 cut(s) | 1064 |
| Bsu15I | ATCGAT | 2 cut(s) | 403, 1089 |
| BsuRI | GGCC | 7 cut(s) | 246, 257, 488, 786, 950, 995, 1150 |
| BsuTUI | ATCGAT | 2 cut(s) | 403, 1089 |
| BtgI | CCRYGG | 2 cut(s) | 865, 967 |
| BtsCI | GGATG | 5 cut(s) | 118, 226, 448, 702, 762 |
| BtsI | GCAGTG | 1 cut(s) | 633 |
| BtsIMutI | CAGTG | 6 cut(s) | 126, 247, 633, 635, 865, 951 |
| BveI | ACCTGC | 1 cut(s) | 1118 |
| Cac8I | GCNNGC | 1 cut(s) | 273 |
| CfoI | GCGC | 3 cut(s) | 797, 872, 899 |
| Cfr10I | RCCGGY | 1 cut(s) | 995 |
| Cfr13I | GGNCC | 2 cut(s) | 486, 1149 |
| ClaI | ATCGAT | 2 cut(s) | 403, 1089 |
| Csp6I | GTAC | 1 cut(s) | 49 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 952, 987 |
| CviAII | CATG | 7 cut(s) | 168, 197, 370, 508, 523, 751, 866 |
| CviQI | GTAC | 1 cut(s) | 49 |
| DdeI | CTNAG | 4 cut(s) | 162, 248, 345, 765 |
| DpnI | GATC | 5 cut(s) | 402, 406, 540, 627, 1052 |
| DpnII | GATC | 5 cut(s) | 400, 404, 538, 625, 1050 |
| EaeI | YGGCCR | 3 cut(s) | 255, 948, 993 |
| Eco130I | CCWWGG | 1 cut(s) | 865 |
| Eco32I | GATATC | 1 cut(s) | 470 |
| Eco57I | CTGAAG | 3 cut(s) | 57, 384, 408 |
| Eco72I | CACGTG | 1 cut(s) | 544 |
| Eco88I | CYCGRG | 1 cut(s) | 317 |
| EcoRI | GAATTC | 2 cut(s) | 287, 492 |
| EcoRII | CCWGG | 5 cut(s) | 266, 298, 349, 1114, 1145 |
| EcoRV | GATATC | 1 cut(s) | 470 |
| EcoT14I | CCWWGG | 1 cut(s) | 865 |
| ErhI | CCWWGG | 1 cut(s) | 865 |
| Esp3I | CGTCTC | 1 cut(s) | 1159 |
| FaeI | CATG | 7 cut(s) | 171, 200, 373, 511, 526, 754, 869 |
| FatI | CATG | 7 cut(s) | 167, 196, 369, 507, 522, 750, 865 |
| FbaI | TGATCA | 2 cut(s) | 538, 625 |
| FblI | GTMKAC | 1 cut(s) | 1063 |
| Fnu4HI | GCNGC | 2 cut(s) | 6, 447 |
| FokI | GGATG | 5 cut(s) | 125, 233, 455, 689, 769 |
| Fsp4HI | GCNGC | 2 cut(s) | 6, 447 |
| FspBI | CTAG | 1 cut(s) | 983 |
| FspI | TGCGCA | 1 cut(s) | 898 |
| GlaI | GCGC | 3 cut(s) | 796, 871, 898 |
| GluI | GCNGC | 2 cut(s) | 6, 447 |
| GsuI | CTGGAG | 1 cut(s) | 321 |
| HaeIII | GGCC | 7 cut(s) | 246, 257, 488, 786, 950, 995, 1150 |
| HapII | CCGG | 2 cut(s) | 24, 996 |
| HhaI | GCGC | 3 cut(s) | 797, 872, 899 |
| Hin1II | CATG | 7 cut(s) | 171, 200, 373, 511, 526, 754, 869 |
| Hin6I | GCGC | 3 cut(s) | 795, 870, 897 |
| HinP1I | GCGC | 3 cut(s) | 795, 870, 897 |
| HincII | GTYRAC | 3 cut(s) | 700, 820, 1028 |
| HindII | GTYRAC | 3 cut(s) | 700, 820, 1028 |
| HindIII | AAGCTT | 1 cut(s) | 189 |
| HinfI | GANTC | 4 cut(s) | 460, 739, 775, 1076 |
| HpaI | GTTAAC | 1 cut(s) | 820 |
| HpaII | CCGG | 2 cut(s) | 24, 996 |
| HphI | GGTGA | 1 cut(s) | 365 |
| Hpy166II | GTNNAC | 6 cut(s) | 547, 633, 700, 820, 1028, 1064 |
| Hpy188I | TCNGA | 1 cut(s) | 768 |
| Hpy188III | TCNNGA | 5 cut(s) | 35, 434, 652, 688, 1162 |
| Hpy8I | GTNNAC | 6 cut(s) | 547, 633, 700, 820, 1028, 1064 |
| HpyAV | CCTTC | 4 cut(s) | 116, 238, 383, 1172 |
| HpyCH4III | ACNGT | 3 cut(s) | 860, 955, 968 |
| HpyCH4IV | ACGT | 3 cut(s) | 543, 549, 696 |
| HpyCH4V | TGCA | 7 cut(s) | 196, 237, 574, 633, 638, 1010, 1107 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 243, 263, 272, 792, 801, 894, 992 |
| HpyF3I | CTNAG | 4 cut(s) | 162, 248, 345, 765 |
| HpySE526I | ACGT | 3 cut(s) | 543, 549, 696 |
| Hsp92II | CATG | 7 cut(s) | 171, 200, 373, 511, 526, 754, 869 |
| HspAI | GCGC | 3 cut(s) | 795, 870, 897 |
| Ksp22I | TGATCA | 2 cut(s) | 538, 625 |
| KspAI | GTTAAC | 1 cut(s) | 820 |
| Kzo9I | GATC | 5 cut(s) | 400, 404, 538, 625, 1050 |
| LmnI | GCTCC | 3 cut(s) | 69, 801, 893 |
| LweI | GCATC | 5 cut(s) | 214, 246, 331, 433, 747 |
| MaeI | CTAG | 1 cut(s) | 983 |
| MaeII | ACGT | 3 cut(s) | 543, 549, 696 |
| MalI | GATC | 5 cut(s) | 402, 406, 540, 627, 1052 |
| MboI | GATC | 5 cut(s) | 400, 404, 538, 625, 1050 |
| MboII | GAAGA | 7 cut(s) | 155, 187, 296, 377, 406, 509, 1085 |
| MfeI | CAATTG | 1 cut(s) | 989 |
| MflI | RGATCY | 1 cut(s) | 1050 |
| MhlI | GDGCHC | 1 cut(s) | 635 |
| MlsI | TGGCCA | 1 cut(s) | 950 |
| MluNI | TGGCCA | 1 cut(s) | 950 |
| MmeI | TCCRAC | 1 cut(s) | 1164 |
| Mox20I | TGGCCA | 1 cut(s) | 950 |
| MroXI | GAANNNNTTC | 1 cut(s) | 413 |
| MscI | TGGCCA | 1 cut(s) | 950 |
| MseI | TTAA | 4 cut(s) | 479, 819, 926, 1016 |
| MslI | CAYNNNNRTG | 1 cut(s) | 755 |
| Msp20I | TGGCCA | 1 cut(s) | 950 |
| MspI | CCGG | 2 cut(s) | 24, 996 |
| MspR9I | CCNGG | 5 cut(s) | 268, 300, 351, 1116, 1147 |
| MunI | CAATTG | 1 cut(s) | 989 |
| MvaI | CCWGG | 5 cut(s) | 268, 300, 351, 1116, 1147 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 243, 263, 272, 792, 801, 894, 992 |
| NcoI | CCATGG | 1 cut(s) | 865 |
| NdeII | GATC | 5 cut(s) | 400, 404, 538, 625, 1050 |
| NlaIII | CATG | 7 cut(s) | 171, 200, 373, 511, 526, 754, 869 |
| NlaIV | GGNNCC | 1 cut(s) | 9 |
| NsbI | TGCGCA | 1 cut(s) | 898 |
| NspI | RCATGY | 1 cut(s) | 200 |
| PaeR7I | CTCGAG | 1 cut(s) | 317 |
| PasI | CCCWGGG | 1 cut(s) | 1115 |
| PdmI | GAANNNNTTC | 1 cut(s) | 413 |
| PfeI | GAWTC | 4 cut(s) | 460, 739, 775, 1076 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 424 |
| PkrI | GCNGC | 2 cut(s) | 7, 448 |
| Ple19I | CGATCG | 1 cut(s) | 407 |
| PmaCI | CACGTG | 1 cut(s) | 544 |
| PmlI | CACGTG | 1 cut(s) | 544 |
| Ppu21I | YACGTR | 1 cut(s) | 544 |
| Psp1406I | AACGTT | 1 cut(s) | 696 |
| Psp6I | CCWGG | 5 cut(s) | 266, 298, 349, 1114, 1145 |
| PspCI | CACGTG | 1 cut(s) | 544 |
| PspGI | CCWGG | 5 cut(s) | 266, 298, 349, 1114, 1145 |
| PspN4I | GGNNCC | 1 cut(s) | 9 |
| PspPI | GGNCC | 2 cut(s) | 486, 1149 |
| PspXI | VCTCGAGB | 1 cut(s) | 317 |
| PsuI | RGATCY | 1 cut(s) | 1050 |
| PvuI | CGATCG | 1 cut(s) | 407 |
| RsaI | GTAC | 1 cut(s) | 50 |
| RsaNI | GTAC | 1 cut(s) | 49 |
| RseI | CAYNNNNRTG | 1 cut(s) | 755 |
| SaqAI | TTAA | 4 cut(s) | 479, 819, 926, 1016 |
| SatI | GCNGC | 2 cut(s) | 6, 447 |
| Sau3AI | GATC | 5 cut(s) | 400, 404, 538, 625, 1050 |
| Sau96I | GGNCC | 2 cut(s) | 486, 1149 |
| ScaI | AGTACT | 1 cut(s) | 50 |
| ScrFI | CCNGG | 5 cut(s) | 268, 300, 351, 1116, 1147 |
| SduI | GDGCHC | 1 cut(s) | 635 |
| SfaNI | GCATC | 5 cut(s) | 214, 246, 331, 433, 747 |
| SfcI | CTRYAG | 1 cut(s) | 1140 |
| Sfr274I | CTCGAG | 1 cut(s) | 317 |
| SlaI | CTCGAG | 1 cut(s) | 317 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 755 |
| SmlI | CTYRAG | 3 cut(s) | 44, 317, 1162 |
| SmoI | CTYRAG | 3 cut(s) | 44, 317, 1162 |
| SsiI | CCGC | 3 cut(s) | 5, 447, 754 |
| SspMI | CTAG | 1 cut(s) | 983 |
| StyD4I | CCNGG | 5 cut(s) | 266, 298, 349, 1114, 1145 |
| StyI | CCWWGG | 1 cut(s) | 865 |
| TaaI | ACNGT | 3 cut(s) | 860, 955, 968 |
| TaiI | ACGT | 3 cut(s) | 546, 552, 699 |
| TaqI | TCGA | 6 cut(s) | 291, 318, 403, 407, 909, 1089 |
| TatI | WGTACW | 1 cut(s) | 48 |
| TauI | GCSGC | 2 cut(s) | 8, 449 |
| TfiI | GAWTC | 4 cut(s) | 460, 739, 775, 1076 |
| Tru1I | TTAA | 4 cut(s) | 479, 819, 926, 1016 |
| Tru9I | TTAA | 4 cut(s) | 479, 819, 926, 1016 |
| TscAI | CASTG | 6 cut(s) | 133, 247, 635, 640, 865, 958 |
| TspDTI | ATGAA | 5 cut(s) | 372, 386, 579, 596, 1086 |
| TspGWI | ACGGA | 1 cut(s) | 1183 |
| TspRI | CASTG | 6 cut(s) | 133, 247, 635, 640, 865, 958 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 424 |
| VneI | GTGCAC | 1 cut(s) | 631 |
| XapI | RAATTY | 4 cut(s) | 287, 409, 492, 905 |
| XceI | RCATGY | 1 cut(s) | 200 |
| XhoI | CTCGAG | 1 cut(s) | 317 |
| XmiI | GTMKAC | 1 cut(s) | 1063 |
| XmnI | GAANNNNTTC | 1 cut(s) | 413 |
| XspI | CTAG | 1 cut(s) | 983 |
| ZrmI | AGTACT | 1 cut(s) | 50 |