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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 1991 |
| AccII | CGCG | 2 cut(s) | 232, 316 |
| AccIII | TCCGGA | 1 cut(s) | 458 |
| AciI | CCGC | 4 cut(s) | 230, 314, 1145, 1236 |
| AclWI | GGATC | 6 cut(s) | 16, 450, 463, 1866, 2144, 2157 |
| AcoI | YGGCCR | 2 cut(s) | 1165, 1608 |
| AcsI | RAATTY | 1 cut(s) | 2008 |
| AcuI | CTGAAG | 3 cut(s) | 942, 1425, 1834 |
| AcyI | GRCGYC | 1 cut(s) | 1227 |
| AfaI | GTAC | 6 cut(s) | 213, 297, 319, 522, 629, 1881 |
| AfeI | AGCGCT | 1 cut(s) | 1743 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 510, 1027 |
| AflII | CTTAAG | 1 cut(s) | 1745 |
| AgsI | TTSAA | 7 cut(s) | 153, 237, 836, 1421, 1780, 1939, 2013 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1500 |
| AjiI | CACGTC | 2 cut(s) | 18, 1829 |
| AjnI | CCWGG | 1 cut(s) | 1167 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1401, 1433 |
| Alw26I | GTCTC | 1 cut(s) | 550 |
| AlwI | GGATC | 6 cut(s) | 16, 450, 463, 1866, 2144, 2157 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1532 |
| Aor13HI | TCCGGA | 1 cut(s) | 458 |
| Aor51HI | AGCGCT | 1 cut(s) | 1743 |
| AoxI | GGCC | 4 cut(s) | 1165, 1586, 1608, 1788 |
| ApeKI | GCWGC | 2 cut(s) | 125, 1199 |
| ApoI | RAATTY | 1 cut(s) | 2008 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 413, 445 |
| AseI | ATTAAT | 1 cut(s) | 540 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1449 |
| AspA2I | CCTAGG | 1 cut(s) | 692 |
| AspLEI | GCGC | 6 cut(s) | 193, 277, 568, 763, 1560, 1744 |
| AspS9I | GGNCC | 4 cut(s) | 380, 532, 839, 1788 |
| AsuHPI | GGTGA | 4 cut(s) | 1119, 1864, 1907, 2128 |
| AsuII | TTCGAA | 1 cut(s) | 2156 |
| AvaII | GGWCC | 3 cut(s) | 380, 532, 839 |
| AvrII | CCTAGG | 1 cut(s) | 692 |
| BaeGI | GKGCMC | 1 cut(s) | 1996 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1890, 1923 |
| BalI | TGGCCA | 1 cut(s) | 1167 |
| BamHI | GGATCC | 2 cut(s) | 455, 2149 |
| BanI | GGYRCC | 1 cut(s) | 1991 |
| BanII | GRGCYC | 1 cut(s) | 1039 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 301, 333, 1904, 1936 |
| BbsI | GAAGAC | 1 cut(s) | 2136 |
| BbvI | GCAGC | 2 cut(s) | 112, 1186 |
| BccI | CCATC | 5 cut(s) | 490, 740, 942, 988, 1853 |
| BceAI | ACGGC | 2 cut(s) | 876, 1853 |
| BciT130I | CCWGG | 1 cut(s) | 1169 |
| BcoDI | GTCTC | 1 cut(s) | 550 |
| BfmI | CTRYAG | 1 cut(s) | 1614 |
| BfoI | RGCGCY | 3 cut(s) | 569, 764, 1745 |
| BfrI | CTTAAG | 1 cut(s) | 1745 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1043 |
| BisI | GCNGC | 3 cut(s) | 126, 1200, 1236 |
| BlnI | CCTAGG | 1 cut(s) | 692 |
| BlsI | GCNGC | 3 cut(s) | 127, 1201, 1237 |
| Bme1390I | CCNGG | 1 cut(s) | 1169 |
| Bme18I | GGWCC | 3 cut(s) | 380, 532, 839 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1500 |
| BmgBI | CACGTC | 2 cut(s) | 18, 1829 |
| BmgT120I | GGNCC | 4 cut(s) | 380, 532, 839, 1788 |
| BmiI | GGNNCC | 7 cut(s) | 381, 457, 534, 1411, 1993, 2079, 2151 |
| BmrFI | CCNGG | 1 cut(s) | 1169 |
| BmrI | ACTGGG | 3 cut(s) | 357, 686, 1606 |
| BmsI | GCATC | 4 cut(s) | 178, 262, 346, 425 |
| BmuI | ACTGGG | 3 cut(s) | 357, 686, 1606 |
| BpiI | GAAGAC | 1 cut(s) | 2136 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1335 |
| Bpu14I | TTCGAA | 1 cut(s) | 2156 |
| BpuEI | CTTGAG | 2 cut(s) | 1774, 1904 |
| BsaBI | GATNNNNATC | 1 cut(s) | 2026 |
| BsaHI | GRCGYC | 1 cut(s) | 1227 |
| BsaJI | CCNNGG | 4 cut(s) | 528, 692, 1168, 1996 |
| BsaWI | WCCGGW | 1 cut(s) | 458 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1253, 1283 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 510, 1027 |
| Bse118I | RCCGGY | 1 cut(s) | 1606 |
| Bse1I | ACTGG | 5 cut(s) | 363, 681, 1526, 1601, 2206 |
| Bse8I | GATNNNNATC | 1 cut(s) | 2026 |
| BseAI | TCCGGA | 1 cut(s) | 458 |
| BseBI | CCWGG | 1 cut(s) | 1169 |
| BseDI | CCNNGG | 4 cut(s) | 528, 692, 1168, 1996 |
| BseJI | GATNNNNATC | 1 cut(s) | 2026 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 510, 1027 |
| BseMII | CTCAG | 7 cut(s) | 272, 356, 674, 1349, 1455, 2007, 2103 |
| BseNI | ACTGG | 5 cut(s) | 363, 681, 1526, 1601, 2206 |
| BseRI | GAGGAG | 3 cut(s) | 1019, 1274, 1322 |
| BseSI | GKGCMC | 1 cut(s) | 1996 |
| BseXI | GCAGC | 2 cut(s) | 112, 1186 |
| BseYI | CCCAGC | 1 cut(s) | 1324 |
| BsgI | GTGCAG | 1 cut(s) | 791 |
| Bsh1236I | CGCG | 2 cut(s) | 232, 316 |
| BshFI | GGCC | 4 cut(s) | 1167, 1588, 1610, 1790 |
| BshNI | GGYRCC | 1 cut(s) | 1991 |
| BsiSI | CCGG | 2 cut(s) | 459, 1607 |
| BslFI | GGGAC | 5 cut(s) | 393, 518, 1514, 1585, 2106 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 510, 1027 |
| BsmAI | GTCTC | 1 cut(s) | 550 |
| BsmFI | GGGAC | 5 cut(s) | 393, 518, 1514, 1585, 2106 |
| BsmI | GAATGC | 1 cut(s) | 1519 |
| BsnI | GGCC | 4 cut(s) | 1167, 1588, 1610, 1790 |
| Bsp119I | TTCGAA | 1 cut(s) | 2156 |
| Bsp1286I | GDGCHC | 2 cut(s) | 1039, 1996 |
| Bsp13I | TCCGGA | 1 cut(s) | 458 |
| Bsp143I | GATC | 8 cut(s) | 8, 455, 1153, 1858, 1962, 2021, 2027, 2149 |
| BspACI | CCGC | 4 cut(s) | 230, 314, 1145, 1236 |
| BspANI | GGCC | 4 cut(s) | 1167, 1588, 1610, 1790 |
| BspCNI | CTCAG | 7 cut(s) | 271, 355, 673, 1348, 1454, 2008, 2102 |
| BspEI | TCCGGA | 1 cut(s) | 458 |
| BspFNI | CGCG | 2 cut(s) | 232, 316 |
| BspHI | TCATGA | 1 cut(s) | 1927 |
| BspLI | GGNNCC | 7 cut(s) | 381, 457, 534, 1411, 1993, 2079, 2151 |
| BspPI | GGATC | 6 cut(s) | 16, 450, 463, 1866, 2144, 2157 |
| BspQI | GCTCTTC | 1 cut(s) | 804 |
| BspT104I | TTCGAA | 1 cut(s) | 2156 |
| BspT107I | GGYRCC | 1 cut(s) | 1991 |
| BspTI | CTTAAG | 1 cut(s) | 1745 |
| BsrFI | RCCGGY | 1 cut(s) | 1606 |
| BsrI | ACTGG | 5 cut(s) | 363, 681, 1526, 1601, 2206 |
| BssAI | RCCGGY | 1 cut(s) | 1606 |
| BssECI | CCNNGG | 4 cut(s) | 528, 692, 1168, 1996 |
| BssMI | GATC | 8 cut(s) | 8, 455, 1153, 1858, 1962, 2021, 2027, 2149 |
| BssNI | GRCGYC | 1 cut(s) | 1227 |
| BssT1I | CCWWGG | 3 cut(s) | 528, 692, 1996 |
| Bst2UI | CCWGG | 1 cut(s) | 1169 |
| Bst4CI | ACNGT | 4 cut(s) | 1303, 1357, 1618, 2113 |
| Bst6I | CTCTTC | 2 cut(s) | 804, 2153 |
| BstACI | GRCGYC | 1 cut(s) | 1227 |
| BstAFI | CTTAAG | 1 cut(s) | 1745 |
| BstBI | TTCGAA | 1 cut(s) | 2156 |
| BstC8I | GCNNGC | 1 cut(s) | 1197 |
| BstDEI | CTNAG | 8 cut(s) | 258, 342, 569, 660, 1335, 1441, 2016, 2089 |
| BstFNI | CGCG | 2 cut(s) | 232, 316 |
| BstH2I | RGCGCY | 3 cut(s) | 569, 764, 1745 |
| BstHHI | GCGC | 6 cut(s) | 193, 277, 568, 763, 1560, 1744 |
| BstKTI | GATC | 8 cut(s) | 11, 458, 1156, 1861, 1965, 2024, 2030, 2152 |
| BstMAI | GTCTC | 1 cut(s) | 550 |
| BstMBI | GATC | 8 cut(s) | 8, 455, 1153, 1858, 1962, 2021, 2027, 2149 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 125, 769, 1043, 1634, 1741 |
| BstNI | CCWGG | 1 cut(s) | 1169 |
| BstNSI | RCATGY | 2 cut(s) | 445, 1322 |
| BstSCI | CCNGG | 1 cut(s) | 1167 |
| BstSFI | CTRYAG | 1 cut(s) | 1614 |
| BstSLI | GKGCMC | 1 cut(s) | 1996 |
| BstUI | CGCG | 2 cut(s) | 232, 316 |
| BstV1I | GCAGC | 2 cut(s) | 112, 1186 |
| BstV2I | GAAGAC | 1 cut(s) | 2136 |
| BstX2I | RGATCY | 4 cut(s) | 8, 455, 1858, 2149 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1099 |
| BstYI | RGATCY | 4 cut(s) | 8, 455, 1858, 2149 |
| BsuRI | GGCC | 4 cut(s) | 1167, 1588, 1610, 1790 |
| BtrI | CACGTC | 2 cut(s) | 18, 1829 |
| BtsI | GCAGTG | 1 cut(s) | 1676 |
| BtsIMutI | CAGTG | 4 cut(s) | 417, 1353, 1676, 2178 |
| Cac8I | GCNNGC | 1 cut(s) | 1197 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1532 |
| CciI | TCATGA | 1 cut(s) | 1927 |
| CfoI | GCGC | 6 cut(s) | 193, 277, 568, 763, 1560, 1744 |
| Cfr10I | RCCGGY | 1 cut(s) | 1606 |
| Cfr13I | GGNCC | 4 cut(s) | 380, 532, 839, 1788 |
| CseI | GACGC | 1 cut(s) | 1216 |
| Csp6I | GTAC | 6 cut(s) | 212, 296, 318, 521, 628, 1880 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1600, 1635 |
| CviAII | CATG | 8 cut(s) | 442, 1093, 1319, 1454, 1928, 1960, 2061, 2146 |
| CviQI | GTAC | 6 cut(s) | 212, 296, 318, 521, 628, 1880 |
| DdeI | CTNAG | 8 cut(s) | 258, 342, 569, 660, 1335, 1441, 2016, 2089 |
| DpnI | GATC | 8 cut(s) | 10, 457, 1155, 1860, 1964, 2023, 2029, 2151 |
| DpnII | GATC | 8 cut(s) | 8, 455, 1153, 1858, 1962, 2021, 2027, 2149 |
| DraI | TTTAAA | 1 cut(s) | 2047 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1500 |
| EaeI | YGGCCR | 2 cut(s) | 1165, 1608 |
| Eam1104I | CTCTTC | 2 cut(s) | 804, 2153 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1500 |
| EarI | CTCTTC | 2 cut(s) | 804, 2153 |
| Eco130I | CCWWGG | 3 cut(s) | 528, 692, 1996 |
| Eco24I | GRGCYC | 1 cut(s) | 1039 |
| Eco32I | GATATC | 1 cut(s) | 2096 |
| Eco47I | GGWCC | 3 cut(s) | 380, 532, 839 |
| Eco47III | AGCGCT | 1 cut(s) | 1743 |
| Eco57I | CTGAAG | 3 cut(s) | 942, 1425, 1834 |
| EcoO109I | RGGNCCY | 2 cut(s) | 532, 839 |
| EcoRII | CCWGG | 1 cut(s) | 1167 |
| EcoRV | GATATC | 1 cut(s) | 2096 |
| EcoT14I | CCWWGG | 3 cut(s) | 528, 692, 1996 |
| EcoT38I | GRGCYC | 1 cut(s) | 1039 |
| ErhI | CCWWGG | 3 cut(s) | 528, 692, 1996 |
| FaeI | CATG | 8 cut(s) | 445, 1096, 1322, 1457, 1931, 1963, 2064, 2149 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1080, 1112 |
| FaqI | GGGAC | 5 cut(s) | 393, 518, 1514, 1585, 2106 |
| FatI | CATG | 8 cut(s) | 441, 1092, 1318, 1453, 1927, 1959, 2060, 2145 |
| FauI | CCCGC | 1 cut(s) | 1152 |
| FauNDI | CATATG | 3 cut(s) | 655, 964, 1760 |
| Fnu4HI | GCNGC | 3 cut(s) | 126, 1200, 1236 |
| FriOI | GRGCYC | 1 cut(s) | 1039 |
| Fsp4HI | GCNGC | 3 cut(s) | 126, 1200, 1236 |
| GlaI | GCGC | 6 cut(s) | 192, 276, 567, 762, 1559, 1743 |
| GluI | GCNGC | 3 cut(s) | 126, 1200, 1236 |
| GsaI | CCCAGC | 1 cut(s) | 1328 |
| HaeII | RGCGCY | 3 cut(s) | 569, 764, 1745 |
| HaeIII | GGCC | 4 cut(s) | 1167, 1588, 1610, 1790 |
| HapII | CCGG | 2 cut(s) | 459, 1607 |
| HgaI | GACGC | 1 cut(s) | 1216 |
| HhaI | GCGC | 6 cut(s) | 193, 277, 568, 763, 1560, 1744 |
| Hin1I | GRCGYC | 1 cut(s) | 1227 |
| Hin1II | CATG | 8 cut(s) | 445, 1096, 1322, 1457, 1931, 1963, 2064, 2149 |
| Hin6I | GCGC | 6 cut(s) | 191, 275, 566, 761, 1558, 1742 |
| HinP1I | GCGC | 6 cut(s) | 191, 275, 566, 761, 1558, 1742 |
| HincII | GTYRAC | 1 cut(s) | 1975 |
| HindII | GTYRAC | 1 cut(s) | 1975 |
| HinfI | GANTC | 5 cut(s) | 500, 1124, 1433, 1943, 2119 |
| HpaII | CCGG | 2 cut(s) | 459, 1607 |
| HphI | GGTGA | 4 cut(s) | 1119, 1864, 1907, 2128 |
| Hpy166II | GTNNAC | 3 cut(s) | 146, 1975, 2203 |
| Hpy188I | TCNGA | 6 cut(s) | 855, 1279, 1444, 1484, 1826, 1853 |
| Hpy188III | TCNNGA | 8 cut(s) | 453, 459, 1360, 1570, 1777, 1928, 2025, 2091 |
| Hpy8I | GTNNAC | 3 cut(s) | 146, 1975, 2203 |
| HpyCH4III | ACNGT | 4 cut(s) | 1303, 1357, 1618, 2113 |
| HpyCH4IV | ACGT | 6 cut(s) | 17, 79, 148, 946, 1828, 2172 |
| HpyCH4V | TGCA | 5 cut(s) | 667, 772, 974, 1318, 1628 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 125, 769, 1043, 1634, 1741 |
| HpyF3I | CTNAG | 8 cut(s) | 258, 342, 569, 660, 1335, 1441, 2016, 2089 |
| HpySE526I | ACGT | 6 cut(s) | 17, 79, 148, 946, 1828, 2172 |
| Hsp92I | GRCGYC | 1 cut(s) | 1227 |
| Hsp92II | CATG | 8 cut(s) | 445, 1096, 1322, 1457, 1931, 1963, 2064, 2149 |
| HspAI | GCGC | 6 cut(s) | 191, 275, 566, 761, 1558, 1742 |
| Kpn2I | TCCGGA | 1 cut(s) | 458 |
| Kzo9I | GATC | 8 cut(s) | 8, 455, 1153, 1858, 1962, 2021, 2027, 2149 |
| LguI | GCTCTTC | 1 cut(s) | 804 |
| LmnI | GCTCC | 1 cut(s) | 364 |
| Lsp1109I | GCAGC | 2 cut(s) | 112, 1186 |
| LweI | GCATC | 4 cut(s) | 178, 262, 346, 425 |
| MaeII | ACGT | 6 cut(s) | 17, 79, 148, 946, 1828, 2172 |
| MaeIII | GTNAC | 7 cut(s) | 1125, 1138, 1282, 1492, 1870, 1984, 2173 |
| MalI | GATC | 8 cut(s) | 10, 457, 1155, 1860, 1964, 2023, 2029, 2151 |
| MboI | GATC | 8 cut(s) | 8, 455, 1153, 1858, 1962, 2021, 2027, 2149 |
| MflI | RGATCY | 4 cut(s) | 8, 455, 1858, 2149 |
| MhlI | GDGCHC | 2 cut(s) | 1039, 1996 |
| MlsI | TGGCCA | 1 cut(s) | 1167 |
| MluCI | AATT | 8 cut(s) | 448, 537, 701, 789, 797, 1116, 1817, 2008 |
| MluNI | TGGCCA | 1 cut(s) | 1167 |
| MlyI | GAGTC | 3 cut(s) | 509, 1133, 2128 |
| MmeI | TCCRAC | 1 cut(s) | 1186 |
| Mox20I | TGGCCA | 1 cut(s) | 1167 |
| MroI | TCCGGA | 1 cut(s) | 458 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1449 |
| MscI | TGGCCA | 1 cut(s) | 1167 |
| MseI | TTAA | 8 cut(s) | 71, 540, 546, 987, 1188, 1746, 2046, 2108 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1097, 1907 |
| Msp20I | TGGCCA | 1 cut(s) | 1167 |
| MspA1I | CMGCKG | 1 cut(s) | 1727 |
| MspCI | CTTAAG | 1 cut(s) | 1745 |
| MspI | CCGG | 2 cut(s) | 459, 1607 |
| MspR9I | CCNGG | 1 cut(s) | 1169 |
| Mva1269I | GAATGC | 1 cut(s) | 1519 |
| MvaI | CCWGG | 1 cut(s) | 1169 |
| MvnI | CGCG | 2 cut(s) | 232, 316 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 125, 769, 1043, 1634, 1741 |
| NdeI | CATATG | 3 cut(s) | 655, 964, 1760 |
| NdeII | GATC | 8 cut(s) | 8, 455, 1153, 1858, 1962, 2021, 2027, 2149 |
| NlaIII | CATG | 8 cut(s) | 445, 1096, 1322, 1457, 1931, 1963, 2064, 2149 |
| NlaIV | GGNNCC | 7 cut(s) | 381, 457, 534, 1411, 1993, 2079, 2151 |
| NmuCI | GTSAC | 5 cut(s) | 1125, 1138, 1492, 1870, 1984 |
| NspI | RCATGY | 2 cut(s) | 445, 1322 |
| NspV | TTCGAA | 1 cut(s) | 2156 |
| PagI | TCATGA | 1 cut(s) | 1927 |
| PciSI | GCTCTTC | 1 cut(s) | 804 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1575 |
| PctI | GAATGC | 1 cut(s) | 1519 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1449 |
| PfeI | GAWTC | 2 cut(s) | 1433, 1943 |
| PkrI | GCNGC | 3 cut(s) | 127, 1201, 1237 |
| PleI | GAGTC | 3 cut(s) | 508, 1132, 2127 |
| PpsI | GAGTC | 3 cut(s) | 508, 1132, 2127 |
| PpuMI | RGGWCCY | 2 cut(s) | 532, 839 |
| PshBI | ATTAAT | 1 cut(s) | 540 |
| Psp5II | RGGWCCY | 2 cut(s) | 532, 839 |
| Psp6I | CCWGG | 1 cut(s) | 1167 |
| PspFI | CCCAGC | 1 cut(s) | 1324 |
| PspGI | CCWGG | 1 cut(s) | 1167 |
| PspN4I | GGNNCC | 7 cut(s) | 381, 457, 534, 1411, 1993, 2079, 2151 |
| PspPI | GGNCC | 4 cut(s) | 380, 532, 839, 1788 |
| PspPPI | RGGWCCY | 2 cut(s) | 532, 839 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1532 |
| PsuI | RGATCY | 4 cut(s) | 8, 455, 1858, 2149 |
| PvuII | CAGCTG | 1 cut(s) | 1727 |
| RsaI | GTAC | 6 cut(s) | 213, 297, 319, 522, 629, 1881 |
| RsaNI | GTAC | 6 cut(s) | 212, 296, 318, 521, 628, 1880 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1097, 1907 |
| SapI | GCTCTTC | 1 cut(s) | 804 |
| SaqAI | TTAA | 8 cut(s) | 71, 540, 546, 987, 1188, 1746, 2046, 2108 |
| SatI | GCNGC | 3 cut(s) | 126, 1200, 1236 |
| Sau3AI | GATC | 8 cut(s) | 8, 455, 1153, 1858, 1962, 2021, 2027, 2149 |
| Sau96I | GGNCC | 4 cut(s) | 380, 532, 839, 1788 |
| SchI | GAGTC | 3 cut(s) | 509, 1133, 2128 |
| ScrFI | CCNGG | 1 cut(s) | 1169 |
| SduI | GDGCHC | 2 cut(s) | 1039, 1996 |
| SfaNI | GCATC | 4 cut(s) | 178, 262, 346, 425 |
| SfcI | CTRYAG | 1 cut(s) | 1614 |
| SfuI | TTCGAA | 1 cut(s) | 2156 |
| SinI | GGWCC | 3 cut(s) | 380, 532, 839 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1097, 1907 |
| SmlI | CTYRAG | 3 cut(s) | 1745, 1753, 1883 |
| SmoI | CTYRAG | 3 cut(s) | 1745, 1753, 1883 |
| Sse9I | AATT | 8 cut(s) | 448, 537, 701, 789, 797, 1116, 1817, 2008 |
| SsiI | CCGC | 4 cut(s) | 230, 314, 1145, 1236 |
| SspI | AATATT | 2 cut(s) | 586, 881 |
| StyD4I | CCNGG | 1 cut(s) | 1167 |
| StyI | CCWWGG | 3 cut(s) | 528, 692, 1996 |
| TaaI | ACNGT | 4 cut(s) | 1303, 1357, 1618, 2113 |
| TaiI | ACGT | 6 cut(s) | 20, 82, 151, 949, 1831, 2175 |
| TaqI | TCGA | 6 cut(s) | 637, 709, 783, 1946, 2026, 2156 |
| TasI | AATT | 8 cut(s) | 448, 537, 701, 789, 797, 1116, 1817, 2008 |
| TatI | WGTACW | 1 cut(s) | 1879 |
| TauI | GCSGC | 1 cut(s) | 1238 |
| TfiI | GAWTC | 2 cut(s) | 1433, 1943 |
| Tru1I | TTAA | 8 cut(s) | 71, 540, 546, 987, 1188, 1746, 2046, 2108 |
| Tru9I | TTAA | 8 cut(s) | 71, 540, 546, 987, 1188, 1746, 2046, 2108 |
| TscAI | CASTG | 4 cut(s) | 417, 1360, 1676, 2185 |
| TseFI | GTSAC | 5 cut(s) | 1125, 1138, 1492, 1870, 1984 |
| TseI | GCWGC | 2 cut(s) | 125, 1199 |
| Tsp45I | GTSAC | 5 cut(s) | 1125, 1138, 1492, 1870, 1984 |
| TspGWI | ACGGA | 4 cut(s) | 263, 347, 661, 1487 |
| TspRI | CASTG | 4 cut(s) | 417, 1360, 1676, 2185 |
| Vha464I | CTTAAG | 1 cut(s) | 1745 |
| VpaK11BI | GGWCC | 3 cut(s) | 380, 532, 839 |
| VspI | ATTAAT | 1 cut(s) | 540 |
| XapI | RAATTY | 1 cut(s) | 2008 |
| XceI | RCATGY | 2 cut(s) | 445, 1322 |
| XcmI | CCANNNNNNNNNTGG | 3 cut(s) | 409, 1420, 1504 |
| XmaJI | CCTAGG | 1 cut(s) | 692 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1449 |