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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 3 cut(s) | 45, 66, 657 |
| AccIII | TCCGGA | 1 cut(s) | 973 |
| AciI | CCGC | 1 cut(s) | 1026 |
| AclWI | GGATC | 3 cut(s) | 965, 995, 1008 |
| AcsI | RAATTY | 2 cut(s) | 405, 568 |
| AcyI | GRCGYC | 1 cut(s) | 1015 |
| AfaI | GTAC | 9 cut(s) | 181, 269, 282, 317, 500, 512, 591, 773, 786 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 539, 951 |
| AgsI | TTSAA | 4 cut(s) | 126, 631, 1106, 1300 |
| AjnI | CCWGG | 1 cut(s) | 271 |
| AluBI | AGCT | 7 cut(s) | 329, 596, 644, 693, 1098, 1282, 1315 |
| AluI | AGCT | 7 cut(s) | 329, 596, 644, 693, 1098, 1282, 1315 |
| Alw21I | GWGCWC | 1 cut(s) | 1100 |
| Alw26I | GTCTC | 2 cut(s) | 550, 977 |
| AlwI | GGATC | 3 cut(s) | 965, 995, 1008 |
| Aor13HI | TCCGGA | 1 cut(s) | 973 |
| ApeKI | GCWGC | 1 cut(s) | 350 |
| ApoI | RAATTY | 2 cut(s) | 405, 568 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 387, 568 |
| AspS9I | GGNCC | 1 cut(s) | 873 |
| AsuC2I | CCSGG | 1 cut(s) | 1023 |
| AsuHPI | GGTGA | 2 cut(s) | 319, 854 |
| AsuII | TTCGAA | 1 cut(s) | 572 |
| AvaII | GGWCC | 1 cut(s) | 873 |
| AxyI | CCTNAGG | 1 cut(s) | 130 |
| BaeGI | GKGCMC | 1 cut(s) | 448 |
| BamHI | GGATCC | 1 cut(s) | 1000 |
| BanII | GRGCYC | 1 cut(s) | 1100 |
| BbsI | GAAGAC | 2 cut(s) | 440, 1018 |
| Bbv12I | GWGCWC | 1 cut(s) | 1100 |
| BbvI | GCAGC | 1 cut(s) | 337 |
| BccI | CCATC | 4 cut(s) | 152, 448, 629, 1138 |
| BciT130I | CCWGG | 1 cut(s) | 273 |
| BcnI | CCSGG | 1 cut(s) | 1023 |
| BcoDI | GTCTC | 2 cut(s) | 550, 977 |
| BfaI | CTAG | 4 cut(s) | 449, 549, 806, 1227 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1023 |
| BisI | GCNGC | 1 cut(s) | 351 |
| BlsI | GCNGC | 1 cut(s) | 352 |
| Bme1390I | CCNGG | 2 cut(s) | 273, 1023 |
| Bme18I | GGWCC | 1 cut(s) | 873 |
| BmgT120I | GGNCC | 1 cut(s) | 873 |
| BmiI | GGNNCC | 3 cut(s) | 748, 875, 1002 |
| BmrFI | CCNGG | 2 cut(s) | 273, 1023 |
| BmsI | GCATC | 3 cut(s) | 241, 337, 950 |
| BpiI | GAAGAC | 2 cut(s) | 440, 1018 |
| Bpu14I | TTCGAA | 1 cut(s) | 572 |
| BpuEI | CTTGAG | 1 cut(s) | 1295 |
| BpuMI | CCSGG | 1 cut(s) | 1023 |
| Bsa29I | ATCGAT | 1 cut(s) | 969 |
| BsaBI | GATNNNNATC | 1 cut(s) | 204 |
| BsaHI | GRCGYC | 1 cut(s) | 1015 |
| BsaI | GGTCTC | 1 cut(s) | 977 |
| BsaJI | CCNNGG | 1 cut(s) | 271 |
| BsaWI | WCCGGW | 1 cut(s) | 973 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 539, 951 |
| Bse21I | CCTNAGG | 1 cut(s) | 130 |
| Bse3DI | GCAATG | 1 cut(s) | 232 |
| Bse8I | GATNNNNATC | 1 cut(s) | 204 |
| BseAI | TCCGGA | 1 cut(s) | 973 |
| BseBI | CCWGG | 1 cut(s) | 273 |
| BseCI | ATCGAT | 1 cut(s) | 969 |
| BseDI | CCNNGG | 1 cut(s) | 271 |
| BseGI | GGATG | 7 cut(s) | 163, 256, 459, 941, 960, 1188, 1305 |
| BseJI | GATNNNNATC | 1 cut(s) | 204 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 539, 951 |
| BseMI | GCAATG | 1 cut(s) | 232 |
| BseMII | CTCAG | 1 cut(s) | 144 |
| BseSI | GKGCMC | 1 cut(s) | 448 |
| BseXI | GCAGC | 1 cut(s) | 337 |
| BseYI | CCCAGC | 1 cut(s) | 829 |
| BshVI | ATCGAT | 1 cut(s) | 969 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1100 |
| BsiSI | CCGG | 2 cut(s) | 974, 1022 |
| BslFI | GGGAC | 1 cut(s) | 859 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 539, 951 |
| BsmAI | GTCTC | 2 cut(s) | 550, 977 |
| BsmFI | GGGAC | 1 cut(s) | 859 |
| BsmI | GAATGC | 1 cut(s) | 1125 |
| Bso31I | GGTCTC | 1 cut(s) | 977 |
| Bsp119I | TTCGAA | 1 cut(s) | 572 |
| Bsp1286I | GDGCHC | 2 cut(s) | 448, 1100 |
| Bsp13I | TCCGGA | 1 cut(s) | 973 |
| Bsp1407I | TGTACA | 3 cut(s) | 179, 315, 498 |
| Bsp143I | GATC | 5 cut(s) | 205, 664, 966, 970, 1000 |
| BspACI | CCGC | 1 cut(s) | 1026 |
| BspCNI | CTCAG | 1 cut(s) | 143 |
| BspDI | ATCGAT | 1 cut(s) | 969 |
| BspEI | TCCGGA | 1 cut(s) | 973 |
| BspLI | GGNNCC | 3 cut(s) | 748, 875, 1002 |
| BspPI | GGATC | 3 cut(s) | 965, 995, 1008 |
| BspT104I | TTCGAA | 1 cut(s) | 572 |
| BspTNI | GGTCTC | 1 cut(s) | 977 |
| BsrDI | GCAATG | 1 cut(s) | 232 |
| BsrGI | TGTACA | 3 cut(s) | 179, 315, 498 |
| BssECI | CCNNGG | 1 cut(s) | 271 |
| BssMI | GATC | 5 cut(s) | 205, 664, 966, 970, 1000 |
| BssNI | GRCGYC | 1 cut(s) | 1015 |
| Bst2UI | CCWGG | 1 cut(s) | 273 |
| Bst4CI | ACNGT | 5 cut(s) | 510, 757, 964, 988, 1152 |
| BstACI | GRCGYC | 1 cut(s) | 1015 |
| BstAUI | TGTACA | 3 cut(s) | 179, 315, 498 |
| BstBI | TTCGAA | 1 cut(s) | 572 |
| BstC8I | GCNNGC | 3 cut(s) | 723, 1123, 1284 |
| BstDEI | CTNAG | 4 cut(s) | 130, 429, 597, 706 |
| BstF5I | GGATG | 7 cut(s) | 163, 256, 459, 941, 960, 1188, 1305 |
| BstKTI | GATC | 5 cut(s) | 208, 667, 969, 973, 1003 |
| BstMAI | GTCTC | 2 cut(s) | 550, 977 |
| BstMBI | GATC | 5 cut(s) | 205, 664, 966, 970, 1000 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 1023, 1232, 1279 |
| BstNI | CCWGG | 1 cut(s) | 273 |
| BstNSI | RCATGY | 2 cut(s) | 476, 480 |
| BstSCI | CCNGG | 2 cut(s) | 271, 1021 |
| BstSLI | GKGCMC | 1 cut(s) | 448 |
| BstV1I | GCAGC | 1 cut(s) | 337 |
| BstV2I | GAAGAC | 2 cut(s) | 440, 1018 |
| BstX2I | RGATCY | 1 cut(s) | 1000 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1247 |
| BstYI | RGATCY | 1 cut(s) | 1000 |
| Bsu15I | ATCGAT | 1 cut(s) | 969 |
| Bsu36I | CCTNAGG | 1 cut(s) | 130 |
| BsuTUI | ATCGAT | 1 cut(s) | 969 |
| BtsCI | GGATG | 7 cut(s) | 163, 256, 459, 941, 960, 1188, 1305 |
| BtsI | GCAGTG | 1 cut(s) | 1050 |
| BtsIMutI | CAGTG | 3 cut(s) | 762, 1050, 1157 |
| Cac8I | GCNNGC | 3 cut(s) | 723, 1123, 1284 |
| Cfr13I | GGNCC | 1 cut(s) | 873 |
| ClaI | ATCGAT | 1 cut(s) | 969 |
| CseI | GACGC | 1 cut(s) | 1023 |
| Csp6I | GTAC | 9 cut(s) | 180, 268, 281, 316, 499, 511, 590, 772, 785 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1135, 1170 |
| CviQI | GTAC | 9 cut(s) | 180, 268, 281, 316, 499, 511, 590, 772, 785 |
| DdeI | CTNAG | 4 cut(s) | 130, 429, 597, 706 |
| DpnI | GATC | 5 cut(s) | 207, 666, 968, 972, 1002 |
| DpnII | GATC | 5 cut(s) | 205, 664, 966, 970, 1000 |
| DraI | TTTAAA | 2 cut(s) | 324, 1069 |
| EciI | GGCGGA | 1 cut(s) | 1041 |
| Ecl136II | GAGCTC | 1 cut(s) | 1098 |
| Eco24I | GRGCYC | 1 cut(s) | 1100 |
| Eco31I | GGTCTC | 1 cut(s) | 977 |
| Eco32I | GATATC | 1 cut(s) | 1186 |
| Eco47I | GGWCC | 1 cut(s) | 873 |
| Eco53kI | GAGCTC | 1 cut(s) | 1098 |
| Eco81I | CCTNAGG | 1 cut(s) | 130 |
| EcoICRI | GAGCTC | 1 cut(s) | 1098 |
| EcoO109I | RGGNCCY | 1 cut(s) | 873 |
| EcoRII | CCWGG | 1 cut(s) | 271 |
| EcoRV | GATATC | 1 cut(s) | 1186 |
| EcoT22I | ATGCAT | 2 cut(s) | 478, 943 |
| EcoT38I | GRGCYC | 1 cut(s) | 1100 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 908, 940 |
| FaqI | GGGAC | 1 cut(s) | 859 |
| Fnu4HI | GCNGC | 1 cut(s) | 351 |
| FokI | GGATG | 7 cut(s) | 170, 263, 466, 928, 967, 1175, 1292 |
| FriOI | GRGCYC | 1 cut(s) | 1100 |
| Fsp4HI | GCNGC | 1 cut(s) | 351 |
| FspBI | CTAG | 4 cut(s) | 449, 549, 806, 1227 |
| GluI | GCNGC | 1 cut(s) | 351 |
| GsaI | CCCAGC | 1 cut(s) | 833 |
| HapII | CCGG | 2 cut(s) | 974, 1022 |
| HgaI | GACGC | 1 cut(s) | 1023 |
| Hin1I | GRCGYC | 1 cut(s) | 1015 |
| HincII | GTYRAC | 2 cut(s) | 960, 1048 |
| HindII | GTYRAC | 2 cut(s) | 960, 1048 |
| HinfI | GANTC | 5 cut(s) | 31, 122, 143, 149, 794 |
| HpaII | CCGG | 2 cut(s) | 974, 1022 |
| HphI | GGTGA | 2 cut(s) | 319, 854 |
| Hpy166II | GTNNAC | 5 cut(s) | 281, 421, 590, 960, 1048 |
| Hpy188I | TCNGA | 4 cut(s) | 148, 196, 220, 361 |
| Hpy188III | TCNNGA | 3 cut(s) | 392, 876, 974 |
| Hpy8I | GTNNAC | 5 cut(s) | 281, 421, 590, 960, 1048 |
| HpyAV | CCTTC | 6 cut(s) | 389, 550, 568, 578, 617, 1028 |
| HpyCH4III | ACNGT | 5 cut(s) | 510, 757, 964, 988, 1152 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 1023, 1232, 1279 |
| HpyF3I | CTNAG | 4 cut(s) | 130, 429, 597, 706 |
| Hsp92I | GRCGYC | 1 cut(s) | 1015 |
| Kpn2I | TCCGGA | 1 cut(s) | 973 |
| Kzo9I | GATC | 5 cut(s) | 205, 664, 966, 970, 1000 |
| LmnI | GCTCC | 1 cut(s) | 1095 |
| Lsp1109I | GCAGC | 1 cut(s) | 337 |
| LweI | GCATC | 3 cut(s) | 241, 337, 950 |
| MaeI | CTAG | 4 cut(s) | 449, 549, 806, 1227 |
| MaeIII | GTNAC | 2 cut(s) | 712, 739 |
| MalI | GATC | 5 cut(s) | 207, 666, 968, 972, 1002 |
| MboI | GATC | 5 cut(s) | 205, 664, 966, 970, 1000 |
| MboII | GAAGA | 9 cut(s) | 131, 380, 445, 478, 527, 674, 1023, 1123, 1312 |
| MfeI | CAATTG | 1 cut(s) | 526 |
| MflI | RGATCY | 1 cut(s) | 1000 |
| MhlI | GDGCHC | 2 cut(s) | 448, 1100 |
| MluCI | AATT | 5 cut(s) | 319, 405, 526, 568, 1260 |
| MlyI | GAGTC | 1 cut(s) | 25 |
| MmeI | TCCRAC | 1 cut(s) | 434 |
| MnlI | CCTC | 9 cut(s) | 139, 241, 418, 594, 711, 762, 864, 1173, 1319 |
| Mph1103I | ATGCAT | 2 cut(s) | 478, 943 |
| MroI | TCCGGA | 1 cut(s) | 973 |
| MroXI | GAANNNNTTC | 2 cut(s) | 387, 568 |
| MseI | TTAA | 6 cut(s) | 27, 234, 323, 681, 789, 1068 |
| MslI | CAYNNNNRTG | 2 cut(s) | 220, 1245 |
| MspI | CCGG | 2 cut(s) | 974, 1022 |
| MspR9I | CCNGG | 2 cut(s) | 273, 1023 |
| MunI | CAATTG | 1 cut(s) | 526 |
| Mva1269I | GAATGC | 1 cut(s) | 1125 |
| MvaI | CCWGG | 1 cut(s) | 273 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 1023, 1232, 1279 |
| NciI | CCSGG | 1 cut(s) | 1023 |
| NdeII | GATC | 5 cut(s) | 205, 664, 966, 970, 1000 |
| NlaIV | GGNNCC | 3 cut(s) | 748, 875, 1002 |
| NsiI | ATGCAT | 2 cut(s) | 478, 943 |
| NspI | RCATGY | 2 cut(s) | 476, 480 |
| NspV | TTCGAA | 1 cut(s) | 572 |
| PctI | GAATGC | 1 cut(s) | 1125 |
| PdmI | GAANNNNTTC | 2 cut(s) | 387, 568 |
| PfeI | GAWTC | 4 cut(s) | 122, 143, 149, 794 |
| PkrI | GCNGC | 1 cut(s) | 352 |
| PleI | GAGTC | 1 cut(s) | 25 |
| PpsI | GAGTC | 1 cut(s) | 25 |
| PpuMI | RGGWCCY | 1 cut(s) | 873 |
| PsiI | TTATAA | 3 cut(s) | 45, 66, 657 |
| Psp124BI | GAGCTC | 1 cut(s) | 1100 |
| Psp5II | RGGWCCY | 1 cut(s) | 873 |
| Psp6I | CCWGG | 1 cut(s) | 271 |
| PspFI | CCCAGC | 1 cut(s) | 829 |
| PspGI | CCWGG | 1 cut(s) | 271 |
| PspN4I | GGNNCC | 3 cut(s) | 748, 875, 1002 |
| PspPI | GGNCC | 1 cut(s) | 873 |
| PspPPI | RGGWCCY | 1 cut(s) | 873 |
| PsuI | RGATCY | 1 cut(s) | 1000 |
| RsaI | GTAC | 9 cut(s) | 181, 269, 282, 317, 500, 512, 591, 773, 786 |
| RsaNI | GTAC | 9 cut(s) | 180, 268, 281, 316, 499, 511, 590, 772, 785 |
| RseI | CAYNNNNRTG | 2 cut(s) | 220, 1245 |
| SacI | GAGCTC | 1 cut(s) | 1100 |
| SaqAI | TTAA | 6 cut(s) | 27, 234, 323, 681, 789, 1068 |
| SatI | GCNGC | 1 cut(s) | 351 |
| Sau3AI | GATC | 5 cut(s) | 205, 664, 966, 970, 1000 |
| Sau96I | GGNCC | 1 cut(s) | 873 |
| SchI | GAGTC | 1 cut(s) | 25 |
| ScrFI | CCNGG | 2 cut(s) | 273, 1023 |
| SduI | GDGCHC | 2 cut(s) | 448, 1100 |
| SfaNI | GCATC | 3 cut(s) | 241, 337, 950 |
| SfuI | TTCGAA | 1 cut(s) | 572 |
| SinI | GGWCC | 1 cut(s) | 873 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 220, 1245 |
| SmlI | CTYRAG | 1 cut(s) | 1310 |
| SmoI | CTYRAG | 1 cut(s) | 1310 |
| Sse9I | AATT | 5 cut(s) | 319, 405, 526, 568, 1260 |
| SsiI | CCGC | 1 cut(s) | 1026 |
| SspMI | CTAG | 4 cut(s) | 449, 549, 806, 1227 |
| SstI | GAGCTC | 1 cut(s) | 1100 |
| StyD4I | CCNGG | 2 cut(s) | 271, 1021 |
| TaaI | ACNGT | 5 cut(s) | 510, 757, 964, 988, 1152 |
| TaqI | TCGA | 3 cut(s) | 137, 572, 969 |
| TasI | AATT | 5 cut(s) | 319, 405, 526, 568, 1260 |
| TatI | WGTACW | 4 cut(s) | 179, 315, 498, 784 |
| TfiI | GAWTC | 4 cut(s) | 122, 143, 149, 794 |
| Tru1I | TTAA | 6 cut(s) | 27, 234, 323, 681, 789, 1068 |
| Tru9I | TTAA | 6 cut(s) | 27, 234, 323, 681, 789, 1068 |
| TscAI | CASTG | 3 cut(s) | 762, 1057, 1157 |
| TseI | GCWGC | 1 cut(s) | 350 |
| TspDTI | ATGAA | 5 cut(s) | 204, 417, 811, 938, 1281 |
| TspGWI | ACGGA | 2 cut(s) | 934, 1108 |
| TspRI | CASTG | 3 cut(s) | 762, 1057, 1157 |
| VpaK11BI | GGWCC | 1 cut(s) | 873 |
| XapI | RAATTY | 2 cut(s) | 405, 568 |
| XceI | RCATGY | 2 cut(s) | 476, 480 |
| XmnI | GAANNNNTTC | 2 cut(s) | 387, 568 |
| XspI | CTAG | 4 cut(s) | 449, 549, 806, 1227 |
| Zsp2I | ATGCAT | 2 cut(s) | 478, 943 |